C Toyoshima

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution
    C Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    Nature 405:647-55. 2000
  2. ncbi request reprint Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 432:361-8. 2004
  3. ncbi request reprint The average conformation at micromolar [Ca2+] of Ca2+-atpase with bound nucleotide differs from that adopted with the transition state analog ADP.AlFx or with AMPPCP under crystallization conditions at millimolar [Ca2+]
    Martin Picard
    Unité de Recherche Associée 2096 CNRS, Service de Biophysique des Fonctions Membranaires Département de Biologie Joliot Curie, CEA and IFR 46 Université Paris Sud, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Biol Chem 280:18745-54. 2005
  4. doi request reprint New crystal structures of PII-type ATPases: excitement continues
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Curr Opin Struct Biol 23:507-14. 2013
  5. doi request reprint Crystal structures of the calcium pump and sarcolipin in the Mg2+-bound E1 state
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 495:260-4. 2013
  6. ncbi request reprint Crystal structure of the calcium pump with a bound ATP analogue
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 430:529-35. 2004
  7. ncbi request reprint Structural basis of ion pumping by Ca2+-ATPase of the sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Annu Rev Biochem 73:269-92. 2004
  8. ncbi request reprint Structural basis of ion pumping by Ca(2+)-ATPase of sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 555:106-10. 2003
  9. ncbi request reprint Crystal structures of Ca2+-ATPase in various physiological states
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Japan
    Ann N Y Acad Sci 986:1-8. 2003
  10. doi request reprint Structural aspects of ion pumping by Ca2+-ATPase of sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Arch Biochem Biophys 476:3-11. 2008

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution
    C Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    Nature 405:647-55. 2000
    ..Comparison with a low-resolution electron density map of the enzyme in the absence of calcium and with biochemical data suggests that large domain movements take place during active transport...
  2. ncbi request reprint Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 432:361-8. 2004
    ..Release of ADP triggers the opening of the lumenal gate and release of phosphate its closure, effected mainly through movement of the A-domain, the actuator of transmembrane gates...
  3. ncbi request reprint The average conformation at micromolar [Ca2+] of Ca2+-atpase with bound nucleotide differs from that adopted with the transition state analog ADP.AlFx or with AMPPCP under crystallization conditions at millimolar [Ca2+]
    Martin Picard
    Unité de Recherche Associée 2096 CNRS, Service de Biophysique des Fonctions Membranaires Département de Biologie Joliot Curie, CEA and IFR 46 Université Paris Sud, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Biol Chem 280:18745-54. 2005
    ..AMPPCP crystalline form might be only indicative of the requirements for further processing of the complex, toward the transition state leading to phosphorylation and Ca2+ occlusion...
  4. doi request reprint New crystal structures of PII-type ATPases: excitement continues
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Curr Opin Struct Biol 23:507-14. 2013
    ..The current status of two other important PII-type ATPases, Na+,K+-ATPase and H+,K+-ATPase, is also briefly described...
  5. doi request reprint Crystal structures of the calcium pump and sarcolipin in the Mg2+-bound E1 state
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 495:260-4. 2013
    ..Thus, the crystal structures reported here fill a gap in the structural elucidation of the reaction cycle and provide a solid basis for understanding the physiological regulation of the calcium pump...
  6. ncbi request reprint Crystal structure of the calcium pump with a bound ATP analogue
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 430:529-35. 2004
    ..This appears to be the mechanism for occluding the bound Ca2+ ions, before releasing them into the lumen of the sarcoplasmic reticulum...
  7. ncbi request reprint Structural basis of ion pumping by Ca2+-ATPase of the sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Annu Rev Biochem 73:269-92. 2004
    ..Furthermore, the roles of critical amino acid residues identified by extensive mutagenesis studies are becoming evident in terms of atomic structure...
  8. ncbi request reprint Structural basis of ion pumping by Ca(2+)-ATPase of sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 555:106-10. 2003
    ..The meanings of the rearrangements of the transmembrane helices and those of the cytoplasmic domains, and the mechanistic roles of the phosphorylation are now becoming clear...
  9. ncbi request reprint Crystal structures of Ca2+-ATPase in various physiological states
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Japan
    Ann N Y Acad Sci 986:1-8. 2003
    ..The meaning of the rearrangement of the transmembrane helices becomes apparent by homology modeling of the Na(+)K(+)-ATPase...
  10. doi request reprint Structural aspects of ion pumping by Ca2+-ATPase of sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Arch Biochem Biophys 476:3-11. 2008
    ..Presented here is a brief structural account of the ion pumping process, which is achieved by a series of very large domain rearrangements...
  11. doi request reprint How Ca2+-ATPase pumps ions across the sarcoplasmic reticulum membrane
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1793:941-6. 2009
    ..Its crystal structures have been determined for 9 different states that cover nearly the entire reaction cycle. Presented here is a brief structural account of the ion pumping process, with emphasis on why the structure has to be so...
  12. ncbi request reprint Structure determination of tubular crystals of membrane proteins. I. Indexing of diffraction patterns
    C Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    Ultramicroscopy 84:1-14. 2000
    ..Presented here is a tutorial on how to index them using (h, k; n) indexing method, which is particularly useful for tubular crystals of membrane proteins...
  13. pmc Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 108:1833-8. 2011
    ..Thus, the crystal structures demonstrate that ATP and its derivatives are highly adaptable to a wide range of site topologies stabilized by a variety of interactions...
  14. ncbi request reprint Structural changes in the calcium pump accompanying the dissociation of calcium
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 418:605-11. 2002
    ..These rearrangements ensure the release of calcium ions into the lumen of sarcoplasmic reticulum and, on the cytoplasmic side, create a pathway for entry of new calcium ions...
  15. pmc Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors
    Mihoko Takahashi
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 104:5800-5. 2007
    ..They also reveal that Ca(2+)-ATPase has considerable plasticity even when fixed by a transmembrane inhibitor, presumably to accommodate thermal fluctuations...
  16. pmc How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 104:19831-6. 2007
    ..In the second step of rotation, positioning of one water molecule couples the hydrolysis of aspartylphosphate to closing of the gate...
  17. pmc Nucleotide recognition by CopA, a Cu+-transporting P-type ATPase
    Takeo Tsuda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    EMBO J 28:1782-91. 2009
    ..These crystal structures lead us to propose a role of the His and a mechanism for removing Mg(2+) from ATP before phosphoryl transfer...
  18. doi request reprint Crystal structure of the sodium-potassium pump at 2.4 A resolution
    Takehiro Shinoda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nature 459:446-50. 2009
    ....
  19. ncbi request reprint Functional role of "N" (nucleotide) and "P" (phosphorylation) domain interactions in the sarcoplasmic reticulum (SERCA) ATPase
    Suming Hua
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113, Japan
    Biochemistry 41:2264-72. 2002
    ..Domain separation is then enhanced by secondary nucleotide binding to the phosphoenzyme, thereby favoring its hydrolytic cleavage...
  20. pmc Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain
    Haruo Ogawa
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 106:13742-7. 2009
    ..According to a homology model for the high affinity state, it is a closure of the binding cavity that confers a high affinity...
  21. ncbi request reprint [Structure and functions of a calcium pump]
    Yuji Sugita
    Tanpakushitsu Kakusan Koso 50:1271-7. 2005
  22. pmc Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+)
    Koji Obara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 102:14489-96. 2005
    ..For this reason, cation countertransport is probably mandatory for all P-type ATPases and possibly accompanies transport of water as well...
  23. pmc Homology modeling of the cation binding sites of Na+K+-ATPase
    Haruo Ogawa
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 99:15977-82. 2002
    ..Our models readily explain many mutational and biochemical results, including different binding stoichiometry and affinities for Na+ and K+...
  24. ncbi request reprint Protonation of the acidic residues in the transmembrane cation-binding sites of the ca(2+) pump
    Yuji Sugita
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 0032, Japan
    J Am Chem Soc 127:6150-1. 2005
    ..The results clearly indicate that the H-bonds formed by protonation of Glu58 and Glu908 provide extra stability for the Ca2+-binding sites of Ca2+ ATPase...
  25. ncbi request reprint Structural changes in the cytoplasmic domain of phospholamban by phosphorylation at Ser16: a molecular dynamics study
    Yuji Sugita
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochemistry 45:11752-61. 2006
    ..This will also be the mechanism for releasing the phosphorylated phospholamban from kinase...
  26. ncbi request reprint Domain organization and movements in heavy metal ion pumps: papain digestion of CopA, a Cu+-transporting ATPase
    Yuta Hatori
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    J Biol Chem 282:25213-21. 2007
    ....
  27. pmc Modeling of the inhibitory interaction of phospholamban with the Ca2+ ATPase
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 100:467-72. 2003
    ..The model can be extended into the cytoplasmic domain so that Lys-3 in PLN can be cross-linked with Lys-397 and Lys-400 in SERCA1a with little unwinding of the N-terminal helix of PLN...
  28. ncbi request reprint Ion pumping by calcium ATPase of sarcoplasmic reticulum
    Chikashi Toyoshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Adv Exp Med Biol 592:295-303. 2007
  29. pmc Intermediate phosphorylation reactions in the mechanism of ATP utilization by the copper ATPase (CopA) of Thermotoga maritima
    Yuta Hatori
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Biol Chem 283:22541-9. 2008
    ..An H479Q mutation (analogous to one found in Wilson disease) renders CopA unable to utilize ATP, whereas phosphorylation by Pi is retained...
  30. ncbi request reprint Functional and structural roles of critical amino acids within the"N", "P", and "A" domains of the Ca2+ ATPase (SERCA) headpiece
    Hailun Ma
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Biochemistry 44:8090-100. 2005
    ....
  31. ncbi request reprint Characterization of Ca2+ ATPase residues involved in substrate and cation binding
    Giuseppe Inesi
    Department of Biochemistry, University of Maryland, Baltimore 21201, USA
    Ann N Y Acad Sci 986:63-71. 2003
    ....
  32. ncbi request reprint Substrate-induced conformational fit and headpiece closure in the Ca2+ATPase (SERCA)
    Hailun Ma
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 278:28938-43. 2003
    ..Mg2+ is not required for the protective effect of nucleotide, even though it is specifically required for the subsequent catalytic reactions...
  33. ncbi request reprint Fe(2+)-catalyzed oxidation and cleavage of sarcoplasmic reticulum ATPase reveals Mg(2+) and Mg(2+)-ATP sites
    Suming Hua
    Institute of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, 21201, USA
    Biochemistry 41:11405-10. 2002
    ..This oxidation is attributed to bound Fe(2+)-ATP complex, as shown by structural modeling of the Mg(2+)-ATP complex at the substrate site...
  34. ncbi request reprint Calcium transport by sarcoplasmic reticulum Ca(2+)-ATPase. Role of the A domain and its C-terminal link with the transmembrane region
    Jesper V Möller
    Department of Biophysics, University of Aarhus, Ole Worms Alle 185, 8000 Aarhus C, Denmark
    J Biol Chem 277:38647-59. 2002
    ....
  35. ncbi request reprint Interaction sites among phospholamban, sarcolipin, and the sarco(endo)plasmic reticulum Ca(2+)-ATPase
    Takashi Morita
    Banting and Best Department of Medical Research, University of Toronto, Charles H Best Institute, 112 College Street, Toronto, Ont, Canada
    Biochem Biophys Res Commun 369:188-94. 2008
    ..These results have led to a revision of our earlier model for the PLN-SLN-SERCA1a complex...
  36. ncbi request reprint Inhibitors bound to Ca(2+)-free sarcoplasmic reticulum Ca(2+)-ATPase lock its transmembrane region but not necessarily its cytosolic region, revealing the flexibility of the loops connecting transmembrane and cytosolic domains
    Cedric Montigny
    CNRS, URA 2096 Protéines Membranaires Transductrices d Energie, F 91191 Gif sur Yvette, France
    Biochemistry 46:15162-74. 2007
    ..2,5-Di-tert-butyl-1,4-dihydroxybenzene or cyclopiazonic acid, inhibitors which also bind in or near the transmembrane region, also produce similar overall effects on Ca2+-free ATPase...
  37. ncbi request reprint Effects of inhibitors on luminal opening of Ca2+ binding sites in an E2P-like complex of sarcoplasmic reticulum Ca22+-ATPase with Be22+-fluoride
    Martin Picard
    Unité de Recherche Associée 2096 CNRS, Service de Biophysique des Fonctions Membranaires Commissariat à l Energie Atomique CEA, 91191 Gif sur Yvette Cedex, France
    J Biol Chem 281:3360-9. 2006
    ....
  38. pmc Concerted conformational effects of Ca2+ and ATP are required for activation of sequential reactions in the Ca2+ ATPase (SERCA) catalytic cycle
    Giuseppe Inesi
    California Pacific Medical Center Research Institute, 475 Brannan Street, San Francisco, California 94107, USA
    Biochemistry 45:13769-78. 2006
    ..Finally, we measured low-affinity ATP binding to stable phosphoenzyme analogues, demonstrating that the E1-P to E2-P transition and the enzyme turnover are accelerated by ATP binding to the phosphoenzyme in exchange for ADP...
  39. ncbi request reprint Specific structural requirements for the inhibitory effect of thapsigargin on the Ca2+ ATPase SERCA
    Cheng Xu
    Department of Biochemistry, University of Maryland School of Medicine, Baltimore, Maryland 21201 1503, USA
    J Biol Chem 279:17973-9. 2004
    ....
  40. ncbi request reprint Conformational fluctuations of the Ca2+-ATPase in the native membrane environment. Effects of pH, temperature, catalytic substrates, and thapsigargin
    Giuseppe Inesi
    California Pacific Medical Center Research Institute, San Francisco, California 94107, USA
    J Biol Chem 283:1189-96. 2008
    ..2Ca(2+) to E2-P transition, thereby disrupting high affinity binding and allowing luminal diffusion of Ca(2+). The E2 state and luminal path closure follow removal of conformational constraint by phosphate...
  41. ncbi request reprint Structure determination of tubular crystals of membrane proteins. IV. Distortion correction and its combined application with real-space averaging and solvent flattening
    Koji Yonekura
    The W M Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, 1700, 4th Street San Francisco, CA 94158 2532, USA
    Ultramicroscopy 107:1141-58. 2007
    ....
  42. pmc Sarcolipin regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholamban
    Michio Asahi
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, Canada M5G 1L6
    Proc Natl Acad Sci U S A 100:5040-5. 2003
    ..Modeling of the PLN/SLN/SERCA1a complex predicts that the regulator binding cavity in the E(2) conformation of SERCA1a can accommodate both SLN and PLN helices, but not two PLN helices...
  43. ncbi request reprint Interaction between tetraethylammonium and permeant cations at the inactivation gate of the HERG potassium channel
    Hirofumi Shimizu
    Department of Physiology, Fukui Medical University, Fukui, 910 1193 Japan
    Jpn J Physiol 53:25-34. 2003
    ..The order of affinity of alkali cations for the inactivation-impeding site, Rb+ > Cs+ > K+, indicated that the selectivity of the site differed significantly from permeation selectivity, K+ > Rb+ > Cs+...
  44. pmc Profile of Chikashi Toyoshima
    Regina Nuzzo
    Proc Natl Acad Sci U S A 103:1165-7. 2006
  45. ncbi request reprint GUI programs for processing individual images in early stages of helical image reconstruction--for high-resolution structure analysis
    Koji Yonekura
    Graduate School of Frontier Biosciences, Osaka University, 3 4 Hikaridai, Seika, Kyoto 619 0237, Japan
    J Struct Biol 144:184-94. 2003
    ..Here also presented is an overview on helical image reconstruction for high-resolution structure analysis...
  46. ncbi request reprint Critical role of Val-304 in conformational transitions that allow Ca2+ occlusion and phosphoenzyme turnover in the Ca2+ transport ATPase
    Cheng Xu
    California Pacific Medical Center Research Institute, San Francisco, California 94107, USA
    J Biol Chem 283:3297-304. 2008
    ..Furthermore, inadequate occlusion of bound Ca(2+) following utilization of ATP in Val-304 side-chain mutations is attributed to inadequate stabilization of the Glu-309 side chain and consequent defect of its gating function...