K Touhara

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi Functional cloning and reconstitution of vertebrate odorant receptors
    K Touhara
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan
    Life Sci 68:2199-206. 2001
  2. ncbi Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor: mapping the odorant-binding site
    Sayako Katada
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    J Neurosci 25:1806-15. 2005
  3. ncbi Induction of c-Fos expression in mouse vomeronasal neurons by sex-specific non-volatile pheromone(s)
    Hiroko Kimoto
    Department of Integrated Biosciences, University of Tokyo, Chiba 277-8562, Japan
    Chem Senses 30:i146-7. 2005
  4. ncbi Odor discrimination by G protein-coupled olfactory receptors
    Kazushige Touhara
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    Microsc Res Tech 58:135-41. 2002
  5. ncbi Deorphanizing vertebrate olfactory receptors: recent advances in odorant-response assays
    Kazushige Touhara
    Department of Integrated Biosciences, University of Tokyo, Chiba 277 8562, Japan
    Neurochem Int 51:132-9. 2007
  6. ncbi Sexual communication via peptide and protein pheromones
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    Curr Opin Pharmacol 8:759-64. 2008
  7. ncbi Insect olfactory receptor complex functions as a ligand-gated ionotropic channel
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    Ann N Y Acad Sci 1170:177-80. 2009
  8. ncbi Sensing odorants and pheromones with chemosensory receptors
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, 277 8562 Japan
    Annu Rev Physiol 71:307-32. 2009
  9. ncbi Molecular bases of odor discrimination: Reconstitution of olfactory receptors that recognize overlapping sets of odorants
    K Kajiya
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 113, Japan
    J Neurosci 21:6018-25. 2001
  10. ncbi Functional identification and reconstitution of an odorant receptor in single olfactory neurons
    K Touhara
    Department of Neurochemistry, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Proc Natl Acad Sci U S A 96:4040-5. 1999

Collaborators

  • Y Yoshihara
  • LESLIE VOSSHALL
  • T Sato
  • K Sato
  • M Tanaka
  • T Sakurai
  • TIMOTHY HOLY
  • Akio Nakamura
  • T Kikusui
  • Maurizio Pellegrino
  • Yuichi Oike
  • Kenzo Hirose
  • Hitoshi Sakano
  • K Mikoshiba
  • Sayako Katada
  • Yuki Oka
  • Nanaho Fukuda
  • Hiroko Kimoto
  • Nami Miura
  • Tatsuro Nakagawa
  • Aya Kato
  • Takao Nakagawa
  • Sachiko Haga
  • Mika Shirasu
  • Masayo Omura
  • Makiko Suwa
  • Hiroshi Kataoka
  • Masato Iwabu
  • Yukio Ishikawa
  • Kana Tanaka
  • Keiichi Yoshikawa
  • Takao Shimizu
  • Takaaki Nishioka
  • K Kajiya
  • Toshimasa Yamauchi
  • Soichiro Matsuda
  • Miki Okada-Iwabu
  • Mamiko Yamaguchi
  • Shigeyuki Namiki
  • Ichizo Nishino
  • Tatsuya Hattori
  • Iseki Takamoto
  • Kumpei Tokuyama
  • Masaaki Funata
  • Hironori Waki
  • Ryo Nakayama
  • Masashi Fukayama
  • Takashi Kadowaki
  • Mitsuhisa Tabata
  • Satoshi Ishii
  • Reiko Kobayakawa
  • Kohjiro Ueki
  • Hitomi Ogata
  • Yukiko K Hayashi
  • Naoto Kubota
  • Naoko Yamauchi
  • Yoshiki Takai
  • Ryohei Yamaoka
  • Atsushi Ochiai
  • Sadahiro Tatsuki
  • Ryuichi Hayashi
  • Yusuke Uda
  • Yukiteru Ono
  • Shunji Nagai
  • Etsuko Ebisui
  • Francesco Nodari
  • Takatsugu Hirokawa
  • Kentaro Yomogida
  • Masaru Okabe
  • Hidenori Watanabe
  • Hisaki Sekine
  • H Kataoka
  • K Inaki
  • T Haga

Detail Information

Publications43

  1. ncbi Functional cloning and reconstitution of vertebrate odorant receptors
    K Touhara
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan
    Life Sci 68:2199-206. 2001
    ....
  2. ncbi Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor: mapping the odorant-binding site
    Sayako Katada
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    J Neurosci 25:1806-15. 2005
    ..The current study reveals the molecular environment of the odorant-binding site, and it further advances the understanding of GPCR pharmacology...
  3. ncbi Induction of c-Fos expression in mouse vomeronasal neurons by sex-specific non-volatile pheromone(s)
    Hiroko Kimoto
    Department of Integrated Biosciences, University of Tokyo, Chiba 277-8562, Japan
    Chem Senses 30:i146-7. 2005
  4. ncbi Odor discrimination by G protein-coupled olfactory receptors
    Kazushige Touhara
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    Microsc Res Tech 58:135-41. 2002
    ..The molecular bases of odor discrimination at the level of olfactory receptors appear to correlate well with the receptive field in the olfactory bulb where the input signal is further processed to create the specific odor maps...
  5. ncbi Deorphanizing vertebrate olfactory receptors: recent advances in odorant-response assays
    Kazushige Touhara
    Department of Integrated Biosciences, University of Tokyo, Chiba 277 8562, Japan
    Neurochem Int 51:132-9. 2007
    ..The conventional ligand-screening approach and the functional cloning strategies in an odorant-directed manner have allowed us to match ORs to the cognate odorants both in vitro and in vivo...
  6. ncbi Sexual communication via peptide and protein pheromones
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    Curr Opin Pharmacol 8:759-64. 2008
    ..The present review will also discuss why, from chemical and ecological points of view, peptide pheromones evolved...
  7. ncbi Insect olfactory receptor complex functions as a ligand-gated ionotropic channel
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    Ann N Y Acad Sci 1170:177-80. 2009
    ..The olfactory signal transduction mechanism in insects is clearly distinct from that in vertebrates and appears to be a unique strategy that insects have acquired to respond to the olfactory environment...
  8. ncbi Sensing odorants and pheromones with chemosensory receptors
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba, 277 8562 Japan
    Annu Rev Physiol 71:307-32. 2009
    ..We review recent progress on chemosensory receptor structure, function, and circuitry in vertebrates and invertebrates from the point of view of the molecular biology and physiology of these sensory systems...
  9. ncbi Molecular bases of odor discrimination: Reconstitution of olfactory receptors that recognize overlapping sets of odorants
    K Kajiya
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 113, Japan
    J Neurosci 21:6018-25. 2001
    ..Odor discrimination is thus determined by differences in the receptive ranges of the odorant receptors that together encode specific odorant molecules...
  10. ncbi Functional identification and reconstitution of an odorant receptor in single olfactory neurons
    K Touhara
    Department of Neurochemistry, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Proc Natl Acad Sci U S A 96:4040-5. 1999
    ..The present approach should enable us to examine not only ligand specificity of an odorant receptor but also receptor specificity and diversity for a particular odorant of interest...
  11. ncbi Binding of multiple ligands to pleckstrin homology domain regulates membrane translocation and enzyme activity of beta-adrenergic receptor kinase
    K Touhara
    Department of Neurochemistry, Faculty of Medicine, The University of Tokyo, Japan
    FEBS Lett 417:243-8. 1997
    ..That protein environment plays an important role in determining the ligand binding characteristics of a particular PH domain highlights the potential risks of inferring mechanisms from studies of isolated PH domains...
  12. ncbi Insect olfactory receptors are heteromeric ligand-gated ion channels
    Koji Sato
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 452:1002-6. 2008
    ..The ligand-gated ion channel formed by an insect OR complex seems to be the basis for a unique strategy that insects have acquired to respond to the olfactory environment...
  13. ncbi Olfactory receptor antagonism between odorants
    Yuki Oka
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    EMBO J 23:120-6. 2004
    ..The current study also provides insight into strategies to modulate perceived odorant quality...
  14. ncbi Structural determinants for membrane trafficking and G protein selectivity of a mouse olfactory receptor
    Sayako Katada
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan
    J Neurochem 90:1453-63. 2004
    ..This information helps clarify the mechanisms that regulate membrane trafficking and G protein interaction of the OR superfamily...
  15. ncbi Insect olfaction: receptors, signal transduction, and behavior
    K Sato
    Department of Integrated Biosciences, The University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba, 277 8562, Japan
    Results Probl Cell Differ 47:121-38. 2009
    ..The understanding of the molecular mechanism of insect olfaction will help in the development of insect repellents for controlling insect pest and vector populations for a wide range of pathogens...
  16. ncbi Odorant receptor map in the mouse olfactory bulb: in vivo sensitivity and specificity of receptor-defined glomeruli
    Yuki Oka
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277-8562, Japan
    Neuron 52:857-69. 2006
    ..Our results provide a methodology to examine in vivo glomerular responses at the receptor level and further help address the long-standing issues of olfactory sensitivity and specificity under physiological conditions...
  17. ncbi Insect sex-pheromone signals mediated by specific combinations of olfactory receptors
    Takao Nakagawa
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    Science 307:1638-42. 2005
    ..The same effects were also observed for general ORs. Both odorant and pheromone signaling pathways are mediated by means of a common mechanism in insects...
  18. ncbi Effects of mutations in pleckstrin homology domain on beta-adrenergic receptor kinase activity in intact cells
    K Touhara
    Department of Neurochemistry, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo, Bunkyo ku, 113, Japan
    Biochem Biophys Res Commun 252:669-74. 1998
    ..This site-directed mutation study provides molecular information on the mechanism and significance of the betaARK PH domain function in the intact cell system...
  19. ncbi Odorant response assays for a heterologously expressed olfactory receptor
    Sayako Katada
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 277-8562, Chiba, Japan
    Biochem Biophys Res Commun 305:964-9. 2003
    ..Various functional assays for a heterologously expressed olfactory receptor reported in this study, are potentially useful for high-throughput ligand screening and functional analyses of hundreds of olfactory receptors...
  20. ncbi Functional characterization of a mouse testicular olfactory receptor and its role in chemosensing and in regulation of sperm motility
    Nanaho Fukuda
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    J Cell Sci 117:5835-45. 2004
    ..The present studies indicate that mouse testicular ORs might play a role in chemoreception during sperm-egg communication and thereby regulate fertilization...
  21. ncbi Mammalian olfactory receptors: pharmacology, G protein coupling and desensitization
    Aya Kato
    Department of Integrated Biosciences, The University of Tokyo, Room 201, 5 1 5, Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Cell Mol Life Sci 66:3743-53. 2009
    ..A greater understanding of the molecular mechanisms underlying OR activity may help in the discovery of agonists and antagonists of ORs, and of GPCRs with potential therapeutic applications...
  22. ncbi Amino acids involved in conformational dynamics and G protein coupling of an odorant receptor: targeting gain-of-function mutation
    Aya Kato
    Department of Integrated Biosciences, The University of Tokyo, Chiba, Japan
    J Neurochem 107:1261-70. 2008
    ..Our results provide insights into how ORs switch from an inactive to an active state, as well as where and how activated ORs interact with G proteins...
  23. ncbi Molecular biology of peptide pheromone production and reception in mice
    Kazushige Touhara
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Adv Genet 59:147-71. 2007
    ..It is also an intriguing aspect to discuss peptide pheromones in the context of the evolutionary importance of species-specific chemical communication...
  24. ncbi In situ Ca2+ imaging of odor responses in a coronal olfactory epithelium slice
    Masayo Omura
    Department of Integrated Biosciences, University of Tokyo, Chiba 277-8562, Japan
    Neuroreport 14:1123-7. 2003
    ..The methodology established in this study is a powerful tool to visualize spatial distributions of odorant responsive neurons at a cellular resolution, and to construct odor maps in a coronal view of the olfactory epithelium...
  25. ncbi An odorant derivative as an antagonist for an olfactory receptor
    Yuki Oka
    Department of Integrated Biosciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
    Chem Senses 29:815-22. 2004
    ..The current study indicates that as odorants age, they decompose or react with other odorants, which in turn affects responsiveness of an olfactory receptor(s)...
  26. ncbi Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile
    Kana Tanaka
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Curr Biol 19:881-90. 2009
    ..Here, we aimed to identify natural chemoattractants and their corresponding olfactory receptors (Ors) involved in silkworm behavior to mulberry leaves...
  27. ncbi Nasal airflow rate affects the sensitivity and pattern of glomerular odorant responses in the mouse olfactory bulb
    Yuki Oka
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    J Neurosci 29:12070-8. 2009
    ..Our results provide important information on in vivo odorant recognition and suggest that odor representation in the OB is not fixed but rather varies significantly depending on the respiratory state...
  28. ncbi Broadly and narrowly tuned odorant receptors are involved in female sex pheromone reception in Ostrinia moths
    Nami Miura
    Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113 8657, Japan
    Insect Biochem Mol Biol 40:64-73. 2010
    ..scapulalis. The findings of the present study are discussed with reference to knowledge on electrophysiological response profiles of olfactory receptor neurons in Ostrinia moths...
  29. ncbi Myr-Ric-8A enhances G(alpha15)-mediated Ca2+ response of vertebrate olfactory receptors
    Keiichi Yoshikawa
    Department of Integrated Biosciences, Rm201, The University of Tokyo, Kashiwa, Chiba, Japan
    Chem Senses 34:15-23. 2009
    ..Our results suggest that Myr-Ric-8A should be helpful in functional characterization of ORs in heterologous cells using Ca(2+) imaging...
  30. ncbi The male mouse pheromone ESP1 enhances female sexual receptive behaviour through a specific vomeronasal receptor
    Sachiko Haga
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Nature 466:118-22. 2010
    ..These findings show that ESP1 is a crucial male pheromone that regulates female reproductive behaviour through a specific receptor in the mouse vomeronasal system...
  31. ncbi [A molecular basis for odorant recognition: olfactory receptor pharmacology]
    Sayako Katada
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan
    Nippon Yakurigaku Zasshi 124:201-9. 2004
    ..The current studies also help understand mechanisms underlying molecular recognition by GPCRs, with implications for therapeutic application...
  32. ncbi Sex-specific peptides from exocrine glands stimulate mouse vomeronasal sensory neurons
    Hiroko Kimoto
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 437:898-901. 2005
    ..Our results indicate that mice respond to sex-specific peptides released from exocrine glands through the vomeronasal system during direct contact...
  33. ncbi [Function of olfactory receptors in non-olfactory tissues]
    Nanaho Fukuda
    Department of Integrated Biosciences, The University of Tokyo, 5-1-5-201 Kashiwanoha, Chiba 277-8562, Japan
    Seikagaku 76:1462-6. 2004
  34. ncbi Adiponectin and AdipoR1 regulate PGC-1alpha and mitochondria by Ca(2+) and AMPK/SIRT1
    Masato Iwabu
    Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Nature 464:1313-9. 2010
    ..Decreased levels of adiponectin and AdipoR1 in obesity may have causal roles in mitochondrial dysfunction and insulin resistance seen in diabetes...
  35. ncbi Developmental expression patterns of testicular olfactory receptor genes during mouse spermatogenesis
    Nanaho Fukuda
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan
    Genes Cells 11:71-81. 2006
    ....
  36. ncbi A male-specific odorant receptor conserved through the evolution of sex pheromones in Ostrinia moth species
    Nami Miura
    Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113 8657, Japan
    Int J Biol Sci 5:319-30. 2009
    ..The present findings suggest that the male-specific receptor of E11-14:OH was acquired before the divergence of the genus Ostrinia, and functionally retained through the evolution of this genus...
  37. ncbi Sex- and strain-specific expression and vomeronasal activity of mouse ESP family peptides
    Hiroko Kimoto
    Department of Integrated Biosciences, The University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Curr Biol 17:1879-84. 2007
    ..The chemosensation of this nonvolatile peptide family by direct contact appears to be one of strategies for sociosexual communication in rodent species...
  38. ncbi [Mechanisms underlying odorant and pheromone reception]
    Kazushige Touhara
    Department of Integrated Bio-sciences, Graduate School of Frontier Sciences, The University of Tokyo, Seimei-to Rm 201, Univ. Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
    Seikagaku 77:1463-74. 2005
  39. ncbi Dimethyl trisulfide as a characteristic odor associated with fungating cancer wounds
    Mika Shirasu
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Biosci Biotechnol Biochem 73:2117-20. 2009
    ..Controlling the production of dimethyl trisulfide should improve quality of life of patients...
  40. ncbi Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori
    Takeshi Sakurai
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 101:16653-8. 2004
    ..These results provide evidence that BmOR-1 is a G protein-coupled sex pheromone receptor that recognizes bombykol...
  41. ncbi Decreased olfactory mucus secretion and nasal abnormality in mice lacking type 2 and type 3 IP3 receptors
    Nanaho Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Eur J Neurosci 27:2665-75. 2008
    ..Our findings suggest that IP3R2 and IP3R3 mediate nasal mucus secretion, which is important for the maintenance of nasal tissue as well as the perception of odors...
  42. ncbi [Molecular mechanisms underlying sex-pheromone reception in insects]
    Takao Nakagawa
    Tanpakushitsu Kakusan Koso 50:1563-70. 2005
  43. ncbi [Progress in research and clinical practice concerning olfactory sensation--importance of the olfactory mucus in the olfactory sense and signal transduction to the brain]
    Kazushige Touhara
    Nippon Jibiinkoka Gakkai Kaiho 111:475-80. 2008