Research Topics
Genomes and GenesSpecies | Yukihide TomariSummaryAffiliation: University of Tokyo Country: Japan Publications
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Publications
Perspective: machines for RNAiYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Genes Dev 19:517-29. 2005..Here, we review our current understanding of how small RNAs are produced, how they are loaded into protein complexes, and how they repress gene expression...
Sorting of Drosophila small silencing RNAsYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 130:299-308. 2007..Thus, in flies small-RNA duplexes are actively sorted into Argonaute-containing complexes according to their intrinsic structures...- Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexesChristian Matranga
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Cell 123:607-20. 2005..For siRNAs, virtually all RISC is assembled through this cleavage-assisted mechanism. In contrast, passenger-strand cleavage is not important for the incorporation of miRNAs that derive from mismatched duplexes... - A protein sensor for siRNA asymmetryYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 306:1377-80. 2004..Thus, R2D2 is both a protein sensor for siRNA thermodynamic asymmetry and a licensing factor for entry of authentic siRNAs into the RNAi pathway... - Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain proteinKlaus Forstemann
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
PLoS Biol 3:e236. 2005..Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis... - RISC assembly defects in the Drosophila RNAi mutant armitageYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 116:831-41. 2004..Our results suggest that armi is required for RISC maturation... - The RNA-induced silencing complex is a Mg2+-dependent endonucleaseDianne S Schwarz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 USA
Curr Biol 14:787-91. 2004..Thus, the RISC-component that mediates endonucleolytic cleavage of the target RNA remains to be identified... - MicroRNA biogenesis: drosha can't cut it without a partnerYukihide Tomari
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Curr Biol 15:R61-4. 2005.... - Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by dicer-1Klaus Forstemann
Gene Center and Munich Center for Integrated Protein Science CiPS M, Ludwig Maximilians Universitat Munchen, Feodor Lynen Str 25, D 81377 Munich, Germany
Cell 130:287-97. 2007..Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2... - piRNAs--the ancient hunters of genome invadersJulia Verena Hartig
Gene Center of the Ludwig Maximilians University, D 81377 Munchen, Germany
Genes Dev 21:1707-13. 2007..We summarize the recent literature on piRNA biogenesis and function with an emphasis on work in Drosophila, where genetics and biochemistry have met very successfully...