Yukihide Tomari


Affiliation: University of Tokyo
Country: Japan


  1. Zhang H, Liu K, Izumi N, Huang H, Ding D, Ni Z, et al. Structural basis for arginine methylation-independent recognition of PIWIL1 by TDRD2. Proc Natl Acad Sci U S A. 2017;114:12483-12488 pubmed publisher
    ..Our work presented here reveals the molecular divergence of the interactions between different Tudor domain proteins and PIWI proteins. ..
  2. Kobayashi H, Shoji K, Kiyokawa K, Negishi L, Tomari Y. Iruka Eliminates Dysfunctional Argonaute by Selective Ubiquitination of Its Empty State. Mol Cell. 2018;: pubmed publisher
    ..Our findings reveal a sophisticated mechanism for the selective degradation of empty AGO that underlies a quality control process to ensure AGO function. ..
  3. Kobayashi H, Tomari Y. RISC assembly: Coordination between small RNAs and Argonaute proteins. Biochim Biophys Acta. 2016;1859:71-81 pubmed publisher
    ..This article is part of a Special Issue entitled: Clues to long noncoding RNA taxonomy1, edited by Dr. Tetsuro Hirose and Dr. Shinichi Nakagawa. ..
  4. Yao C, Sasaki H, Ueda T, Tomari Y, Tadakuma H. Single-Molecule Analysis of the Target Cleavage Reaction by the Drosophila RNAi Enzyme Complex. Mol Cell. 2015;59:125-32 pubmed publisher
    ..Therefore, the release order of the two cleavage fragments is influenced by the stability in each region, in contrast to the unidirectional base pairing propagation from the seed to the 3' supplementary region upon target recognition. ..
  5. Iwakawa H, Tomari Y. The Functions of MicroRNAs: mRNA Decay and Translational Repression. Trends Cell Biol. 2015;25:651-665 pubmed publisher
    ..Here, we review the recent progress in our understanding of how miRNAs mediate translational repression and mRNA decay, and discuss the contributions of the two silencing modes to the overall silencing effect in both kingdoms. ..
  6. Tsuboyama K, Tadakuma H, Tomari Y. Conformational Activation of Argonaute by Distinct yet Coordinated Actions of the Hsp70 and Hsp90 Chaperone Systems. Mol Cell. 2018;70:722-729.e4 pubmed publisher
    ..Our data uncover distinct and coordinated actions of the chaperone machinery, where the Hsp70 system expands the structural ensembles of Ago2 and the Hsp90 system captures and stabilizes the active form. ..
  7. Izumi N, Shoji K, Sakaguchi Y, Honda S, Kirino Y, Suzuki T, et al. Identification and Functional Analysis of the Pre-piRNA 3' Trimmer in Silkworms. Cell. 2016;164:962-73 pubmed publisher
    ..Our results highlight the cooperative action of Trimmer and Papi/Tdrkh in piRNA maturation. ..
  8. Tomari Y, Du T, Zamore P. Sorting of Drosophila small silencing RNAs. Cell. 2007;130:299-308 pubmed
    ..Thus, in flies small-RNA duplexes are actively sorted into Argonaute-containing complexes according to their intrinsic structures. ..
  9. Fukaya T, Iwakawa H, Tomari Y. MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila. Mol Cell. 2014;56:67-78 pubmed publisher
    ..In contrast, direct tethering of GW182 promotes dissociation of both eIF4E and eIF4A. We propose that miRNAs act to block the assembly of the eIF4F complex during translation initiation. ..

More Information


  1. Wang W, Yoshikawa M, Han B, Izumi N, Tomari Y, Weng Z, et al. The initial uridine of primary piRNAs does not create the tenth adenine that Is the hallmark of secondary piRNAs. Mol Cell. 2014;56:708-16 pubmed publisher
  2. Naruse K, Matsuura Suzuki E, Watanabe M, Iwasaki S, Tomari Y. In vitro reconstitution of chaperone-mediated human RISC assembly. RNA. 2018;24:6-11 pubmed publisher
    ..The reconstitution system lays the groundwork for further studies of small RNA-mediated gene silencing in mammals. ..
  3. Iwasaki S, Tomari Y. Reconstitution of RNA Interference Machinery. Methods Mol Biol. 2018;1680:131-143 pubmed publisher
    ..The reconstitution system provides a versatile framework for detailed studies of RISC assembly, including single molecule analysis as described in another chapter in this issue. ..
  4. Sasaki H, Tadakuma H, Tomari Y. Single-Molecule Analysis for RISC Assembly and Target Cleavage. Methods Mol Biol. 2018;1680:145-164 pubmed publisher
    ..In this chapter, we summarize the detailed protocol for single-molecule analysis of chaperone-assisted assembly of fly Ago2-RISC as well as its target cleavage reaction. ..