Hajime Tokuda

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint Biogenesis of outer membranes in Gram-negative bacteria
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:465-73. 2009
  2. ncbi request reprint Sorting of lipoproteins to the outer membrane in E. coli
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1693:5-13. 2004
  3. ncbi request reprint Sorting of lipoproteins to the outer membrane in E. coli
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1694:IN1-9. 2004
  4. ncbi request reprint [Protein trafficking and envelope biogenesis in bacteria]
    Hajime Tokuda
    Tanpakushitsu Kakusan Koso 49:942-9. 2004
  5. doi request reprint Opening and closing of the hydrophobic cavity of LolA coupled to lipoprotein binding and release
    Yuki Oguchi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo
    J Biol Chem 283:25414-20. 2008
  6. ncbi request reprint Diverse effects of phospholipids on lipoprotein sorting and ATP hydrolysis by the ABC transporter LolCDE complex
    Shigehiko Miyamoto
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1768:1848-54. 2007
  7. doi request reprint Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA
    Shoji Watanabe
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Biol Chem 283:25421-7. 2008
  8. ncbi request reprint Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed {beta}-barrel structures
    Naohiro Taniguchi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032
    J Biol Chem 280:34481-8. 2005
  9. pmc Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB
    Suguru Okuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 106:5877-82. 2009
  10. ncbi request reprint A novel ligand bound ABC transporter, LolCDE, provides insights into the molecular mechanisms underlying membrane detachment of bacterial lipoproteins
    Yasuko Ito
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Mol Microbiol 62:1064-75. 2006

Collaborators

Detail Information

Publications57

  1. ncbi request reprint Biogenesis of outer membranes in Gram-negative bacteria
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:465-73. 2009
    ..In contrast, little is known about how phospholipids are transported to the outer membrane...
  2. ncbi request reprint Sorting of lipoproteins to the outer membrane in E. coli
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1693:5-13. 2004
    ..The Lol avoidance signal is speculated to cause the formation of a tight lipoprotein-phosphatidylethanolamine complex that has five acyl chains and therefore cannot be recognized by LolCDE...
  3. ncbi request reprint Sorting of lipoproteins to the outer membrane in E. coli
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1694:IN1-9. 2004
    ..The Lol avoidance signal is speculated to cause the formation of a tight lipoprotein-phosphatidylethanolamine complex that has five acyl chains and therefore cannot be recognized by LolCDE...
  4. ncbi request reprint [Protein trafficking and envelope biogenesis in bacteria]
    Hajime Tokuda
    Tanpakushitsu Kakusan Koso 49:942-9. 2004
  5. doi request reprint Opening and closing of the hydrophobic cavity of LolA coupled to lipoprotein binding and release
    Yuki Oguchi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo
    J Biol Chem 283:25414-20. 2008
    ..These results, taken together, reveal the LolA cycle, in which the hydrophobic cavity undergoes opening and closing upon the binding and release of lipoproteins, respectively...
  6. ncbi request reprint Diverse effects of phospholipids on lipoprotein sorting and ATP hydrolysis by the ABC transporter LolCDE complex
    Shigehiko Miyamoto
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1768:1848-54. 2007
    ..Taken together, these results indicate that three major phospholipids of E. coli differently affect lipoprotein sorting and the activity of LolCDE...
  7. doi request reprint Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA
    Shoji Watanabe
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Biol Chem 283:25421-7. 2008
    ..Taken together, these results indicate that the two Cys residues introduced into LolA function as a redox switch, which regulates the opening and closing of the hydrophobic cavity...
  8. ncbi request reprint Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed {beta}-barrel structures
    Naohiro Taniguchi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032
    J Biol Chem 280:34481-8. 2005
    ....
  9. pmc Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB
    Suguru Okuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 106:5877-82. 2009
    ..The hydrophobic cavities of LolA and LolB were both found to bind lipoproteins inside. These results indicate that the transfer of lipoproteins through Lol proteins occurs in a mouth-to-mouth manner...
  10. ncbi request reprint A novel ligand bound ABC transporter, LolCDE, provides insights into the molecular mechanisms underlying membrane detachment of bacterial lipoproteins
    Yasuko Ito
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Mol Microbiol 62:1064-75. 2006
    ..LolCDE is the first example of an ABC transporter purified with tightly bound native substrates. A single molecule of lipoprotein is found to bind per molecule of the LolCDE complex...
  11. pmc Molecular events involved in a single cycle of ligand transfer from an ATP binding cassette transporter, LolCDE, to a molecular chaperone, LolA
    Naohiro Taniguchi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    J Biol Chem 283:8538-44. 2008
    ..However, a single cycle of lipoprotein transfer occurred from vanadate-treated LolCDE to LolA, indicating that vanadate traps LolCDE at the energized state...
  12. ncbi request reprint Mechanism underlying the inner membrane retention of Escherichia coli lipoproteins caused by Lol avoidance signals
    Takashi Hara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 278:40408-14. 2003
    ..Furthermore, not only modification of the carboxylic acid of Asp at position 2 but also that of the amine of phosphatidylethanolamine abolished the Lol avoidance function. Based on these results, the Lol avoidance mechanism is discussed...
  13. doi request reprint Dissection of LolB function--lipoprotein binding, membrane targeting and incorporation of lipoproteins into lipid bilayers
    Jun Tsukahara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    FEBS J 276:4496-504. 2009
    ..Taken together, these results indicate that lipoprotein binding, membrane targeting and membrane incorporation of lipoproteins are intrinsic functions of LolB...
  14. ncbi request reprint Topology inversion of SecG is essential for cytosolic SecA-dependent stimulation of protein translocation
    Rie Sugai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 282:29540-8. 2007
    ..Moreover, fixation of the inverted SecG topology was found to be dependent on soluble SecA. The dual functions of SecG in protein translocation will be discussed...
  15. pmc Multiple SecA molecules drive protein translocation across a single translocon with SecG inversion
    Kazuhiro Morita
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    J Biol Chem 287:455-64. 2012
    ....
  16. ncbi request reprint Membrane topology and functional importance of the periplasmic region of ABC transporter LolCDE
    Masaki Yasuda
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    Biosci Biotechnol Biochem 73:2310-6. 2009
    ..Inhibition of the release of lipoproteins by the sulfhydryl reagent supported a previous proposal that LolC and LolE have distinct functions...
  17. pmc Large-scale preparation of the homogeneous LolA lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner
    Shoji Watanabe
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    Protein Sci 16:2741-9. 2007
    ..Finally, the LolB depletion caused the accumulation of a significant amount of Pal in the periplasm, thereby establishing the conditions for preparation of the homogeneous LolA-lipoprotein complex...
  18. doi request reprint Real time analysis of lipoprotein transfer from LolA to LolB by means of surface plasmon resonance
    Jun Tsukahara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 583:2987-90. 2009
    ..We report here the detection of lipoprotein transfer in real time by means of surface plasmon resonance. The kinetic parameters of lipoprotein transfer were determined with wild-type LolA and a mutant defective in it...
  19. pmc A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system
    Kazuyuki Tao
    Radioisotope Center, University of Tokyo, 2 11 16 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 192:5657-62. 2010
    ..Moreover, LolCDE overproduction stabilized I93C/F140C and therefore caused an increase in its level. Taken together, these results indicate that oxidized I93C/F140C stably binds to LolCDE, which causes strong envelope stress...
  20. ncbi request reprint Roles of the hydrophobic cavity and lid of LolA in the lipoprotein transfer reaction in Escherichia coli
    Shoji Watanabe
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 281:3335-42. 2006
    ..Taken together, these results indicate that both the hydrophobic cavity and the lid are critically important for not only the binding of lipoproteins but also their transfer...
  21. pmc Genetic analysis of the mode of interplay between an ATPase subunit and membrane subunits of the lipoprotein-releasing ATP-binding cassette transporter LolCDE
    Yasuko Ito
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 188:2856-64. 2006
    ..Moreover, the LolD motif was found to be critical for functional interplay with LolC/E, since some LolD mutations lowered the ATPase activity of LolCDE without affecting that of LolD...
  22. ncbi request reprint Lipoprotein trafficking in Escherichia coli
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, 113 0032, Tokyo, Japan
    Arch Microbiol 182:1-6. 2004
    ..On the basis of the data, we discuss here the mechanism underlying lipoprotein transfer from the inner to the outer membrane through Lol proteins. We also discuss why inner membrane-specific lipoproteins remain on the inner membrane...
  23. ncbi request reprint Targeted mutagenesis of five conserved tryptophan residues of LolB involved in membrane localization of Escherichia coli lipoproteins
    Rieko Wada
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochem Biophys Res Commun 323:1069-74. 2004
    ..Despite the strong conservation, no defective mutant for Trp at position 183 was obtained, and only weak mutants were isolated for Trp at position 18. Based on the crystal structure of LolB, the phenotypes of these mutants are discussed...
  24. pmc Elucidation of the function of lipoprotein-sorting signals that determine membrane localization
    Kazuhiro Masuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 99:7390-5. 2002
    ..These results revealed a previously undocumented function of aspartate at position 2, i.e., lipoproteins having this signal avoid being recognized by LolCDE, thereby remaining in the inner membrane...
  25. ncbi request reprint Membrane topology inversion of SecG detected by labeling with a membrane-impermeable sulfhydryl reagent that causes a close association of SecG with SecA
    Shushi Nagamori
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biochem 132:629-34. 2002
    ..Thus, the two most dynamic components of the translocation machinery were found for the first time to interact with each other when both undergo conformational changes...
  26. ncbi request reprint Amino acids at positions 3 and 4 determine the membrane specificity of Pseudomonas aeruginosa lipoproteins
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Biol Chem 282:13372-8. 2007
    ..coli lipoproteins, these results suggest a new "+3, +4 rule" for lipoprotein sorting in P. aeruginosa, namely, the final destination of lipoproteins is determined by the residues at positions 3 and 4...
  27. doi request reprint A short helix in the C-terminal region of LolA is important for the specific membrane localization of lipoproteins
    Suguru Okuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    FEBS Lett 582:2247-51. 2008
    ..Thus, the membrane localization of lipoproteins with the LolA derivative lacking the 3(10) helix was independent of LolB whereas LolB was essential for the outer membrane localization of lipoproteins with the wild-type LolA...
  28. doi request reprint Development of a functional in vitro integration system for an integral membrane protein, SecG
    Ken ichi Nishiyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Biochem Biophys Res Commun 390:920-4. 2009
    ..We also present evidence that the membrane targeting and integration of SecG is stimulated by externally added SecA and SecG itself...
  29. ncbi request reprint Aminoacylation of the N-terminal cysteine is essential for Lol-dependent release of lipoproteins from membranes but does not depend on lipoprotein sorting signals
    Ayumu Fukuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 277:43512-8. 2002
    ..Furthermore, lipoproteins with aspartate at position 2 were found to be aminoacylated both in vivo and in vitro, indicating that the lipoprotein-sorting signal does not affect lipid modification...
  30. ncbi request reprint An ABC transporter mediating the membrane detachment of bacterial lipoproteins depending on their sorting signals
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Tokyo 113 0032, Japan
    FEBS Lett 580:1164-70. 2006
    ..LolCDE does not recognize lipoproteins possessing Asp at position 2, which therefore remain anchored to the inner membrane. We will discuss the mechanism of LolCDE based on data obtained through in vitro experiments...
  31. ncbi request reprint Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in quality control in the Escherichia coli periplasm
    Hidenori Miyadai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Biol Chem 279:39807-13. 2004
    ..Construction of various YafY-YfjS chimeric lipoproteins revealed that only a few residues located in the N- and C-terminal regions were important for the induction of DegP...
  32. ncbi request reprint Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits
    Kyoko Kanamaru
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    FEBS J 274:3034-43. 2007
    ..We report here the complete reconstitution of a functional ATP-binding cassette transporter from separately purified subunits...
  33. ncbi request reprint A mutation in the membrane subunit of an ABC transporter LolCDE complex causing outer membrane localization of lipoproteins against their inner membrane-specific signals
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Mol Microbiol 49:167-77. 2003
    ..Taken together, these results indicate that the mutation causes destabilization of the LolCDE complex and concomitantly prevents the accurate recognition of lipoprotein-sorting signals...
  34. doi request reprint Sorting of bacterial lipoproteins to the outer membrane by the Lol system
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Methods Mol Biol 619:117-29. 2010
    ..This complex traverses the hydrophilic periplasm to reach the outer membrane, where LolB accepts a lipoprotein from LolA and then catalyzes its incorporation into the inner leaflet of the outer membrane...
  35. pmc Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Bacteriol 193:4832-40. 2011
    ..These results indicate that the affinity of LolCDE for apolipoprotein is very low, and therefore, overexpression of LolCDE is required for its release and sorting to the outer membrane...
  36. doi request reprint A novel complete reconstitution system for membrane integration of the simplest membrane protein
    Ken ichi Nishiyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochem Biophys Res Commun 394:733-6. 2010
    ..Based on the function of the factor, we propose that the factor is named MPIase (Membrane Protein Integrase)...
  37. doi request reprint Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    FEBS Lett 583:2160-4. 2009
    ..We report here that both LptF and LptG formed a complex with LptB. Furthermore, an inner membrane protein, LptC, which had been implicated in lipopolysaccharide transport, was also included in this complex...
  38. ncbi request reprint Disruption of rpmJ encoding ribosomal protein L36 decreases the expression of secY upstream of the spc operon and inhibits protein translocation in Escherichia coli
    Ayao Ikegami
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 69:1595-602. 2005
    ..These phenotypes caused by the rpmJ null mutation were corrected by a plasmid carrying secY, but not by one carrying rpmJ...
  39. ncbi request reprint Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism
    Shin Ya Tanaka
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Biol Chem 282:13379-84. 2007
    ..aeruginosa. These results indicate that Lol proteins are responsible for the sorting of lipoproteins to the outer membrane of P. aeruginosa, as in the case of E. coli, but respond differently to inner membrane retention signals...
  40. pmc Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system
    Kazuyuki Tao
    Radioisotope Center, University of Tokyo, Tokyo, Japan
    J Bacteriol 194:3643-50. 2012
    ....
  41. ncbi request reprint Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction
    Yoshihisa Kato
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 550:114-8. 2003
    ..SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG...
  42. pmc Diacylglycerol specifically blocks spontaneous integration of membrane proteins and allows detection of a factor-assisted integration
    Yosuke Kawashima
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    J Biol Chem 283:24489-96. 2008
    ..We also demonstrated that the blockage of spontaneous integration by DAG is also operative in native membrane vesicles...
  43. ncbi request reprint Dominant negative mutant of a lipoprotein-specific molecular chaperone, LolA, tightly associates with LolCDE
    Atsuki Miyamoto
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Japan
    FEBS Lett 528:193-6. 2002
    ..This tight association of LolA(F47E) with LolCDE caused a dominant negative phenotype in vivo, suggesting that the LolA-LolCDE interaction is critical for lipoprotein release...
  44. ncbi request reprint Genes coding for SecG and Leu2-tRNA form an operon to give an unusual RNA comprising mRNA and a tRNA precursor
    Ken ichi Nishiyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Biochim Biophys Acta 1729:166-73. 2005
    ..The gene structure of secG-leuU was conserved among Gram-negative bacteria, although the sequences separating the two genes were quite diverse. The physiological significance of this unusual gene organization is discussed...
  45. ncbi request reprint [Protein localization in bacteria]
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032
    Seikagaku 75:109-18. 2003
  46. doi request reprint Novel mutations of the LolCDE complex causing outer membrane localization of lipoproteins despite their inner membrane-retention signals
    Chihiro Sakamoto
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113 0032, Japan
    Biochem Biophys Res Commun 401:586-91. 2010
    ..Based on these observations, the molecular events underlying the recognition of lipoproteins by the LolCDE complex are discussed...
  47. pmc Overexpression of gnsA, a multicopy suppressor of the secG null mutation, increases acidic phospholipid contents by inhibiting phosphatidylethanolamine synthesis at low temperatures
    Rie Sugai
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 186:5968-71. 2004
    ..GnsA overproduction at 20 degrees C specifically inhibited phosphatidylethanolamine synthesis and therefore caused the increase in the proportion of acidic phospholipids...
  48. pmc Disruption of lolCDE, encoding an ATP-binding cassette transporter, is lethal for Escherichia coli and prevents release of lipoproteins from the inner membrane
    Shin ichiro Narita
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    J Bacteriol 184:1417-22. 2002
    ..From these results, we conclude that the LolCDE complex is an essential ABC transporter for E. coli and the sole apparatus mediating the release of outer membrane lipoproteins from the inner membrane...
  49. ncbi request reprint A derivative of lipid A is involved in signal recognition particle/SecYEG-dependent and -independent membrane integrations
    Ken ichi Nishiyama
    Institute of Biochemistry and Molecular Biology, Centre of Biochemistry and Molecular Cell Research, University of Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    J Biol Chem 281:35667-76. 2006
    ..This factor was found to be a lipid A-derived membrane component possessing a peptide moiety...
  50. ncbi request reprint [Localization of lipoproteins in Escherichia coli]
    Kyoko Kanamaru
    Tanpakushitsu Kakusan Koso 49:961-4. 2004
  51. ncbi request reprint [Transport of outer membrane proteins]
    Shin Ichi Matsuyama
    Tanpakushitsu Kakusan Koso 49:965-6. 2004
  52. ncbi request reprint A practical phasing procedure using the MAD method without the aid of XAFS measurements: successful solution in the structure determination of the outer-membrane lipoprotein carrier LolA
    Kazuki Takeda
    RIKEN Harima Institute SPring 8, Koto 1 1 1, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
    Acta Crystallogr D Biol Crystallogr 59:1440-6. 2003
    ..The subsequent MAD phasing gave a high-quality initial electron-density map which was sufficient to construct a complete molecular model of the LolA protein...
  53. pmc Crystal structures of bacterial lipoprotein localization factors, LolA and LolB
    Kazuki Takeda
    RIKEN Harima Institute SPring 8, Koto 1 1 1, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
    EMBO J 22:3199-209. 2003
    ..The results are discussed in the context of the current model for the lipoprotein transfer from the inner to the outer membrane through a hydrophilic environment...
  54. pmc Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli
    Yu Hirano
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1227-30. 2006
    ..The monoclinic crystals diffracted to 3.0 A resolution and belong to space group C2. Initial phases were obtained from a tetragonal crystal of selenomethionylated protein by the MAD method...
  55. ncbi request reprint A new screening method to identify inhibitors of the Lol (localization of lipoproteins) system, a novel antibacterial target
    Hideaki Ito
    Pharmacology Research Laboratories, Dainippon Sumitomo Pharma Co Ltd, Suita, Osaka, Japan
    Microbiol Immunol 51:263-70. 2007
    ..We therefore conclude that our new screening method can efficiently identify new antibacterial agents that target the Lol system...
  56. ncbi request reprint Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli
    Yu Hirano
    Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Structure 15:963-76. 2007
    ..Furthermore, two monomers in the asymmetric unit form an unusual 3D domain-swapped dimer. These findings indicate that tertiary and/or quaternary structural instability may be responsible for Cpx pathway activation...
  57. ncbi request reprint Crystallization and preliminary crystallographic study of the outer-membrane lipoprotein receptor LolB, a member of the lipoprotein localization factors
    Kazuki Takeda
    RIKEN Harima Institute SPring 8, Koto 1 1 1, Mikazuki cho, Sayo gun, Hyogo 679 5148, Japan
    Acta Crystallogr D Biol Crystallogr 59:1224-6. 2003
    ..A four-wavelength data set was collected from a monoclinic crystal of selenomethionylated mLolB in order to perform MAD phasing. The quality of the initial electron-density map was sufficient to build a molecular model...