Tadaomi Takenawa

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint The WASP-WAVE protein network: connecting the membrane to the cytoskeleton
    Tadaomi Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokane dai, Minato ku, Tokyo 108 8639, Japan
    Nat Rev Mol Cell Biol 8:37-48. 2007
  2. pmc A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42
    M Fukuoka
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Cell Biol 152:471-82. 2001
  3. ncbi request reprint Membrane targeting and remodeling through phosphoinositide-binding domains
    Tadaomi Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    IUBMB Life 58:296-303. 2006
  4. ncbi request reprint Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane
    T Takenawa
    Department of Biology, Institute of Medical Science, University of Tokyo, Japan
    Biochim Biophys Acta 1533:190-206. 2001
  5. ncbi request reprint WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement
    T Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Cell Sci 114:1801-9. 2001
  6. ncbi request reprint N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor
    Kazuhiro Kawamura
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba 263 8522, Japan
    J Biol Chem 279:54862-71. 2004
  7. ncbi request reprint FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation
    Kiyohito Mizutani
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokane dai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 313:468-74. 2004
  8. pmc Optimization of WAVE2 complex-induced actin polymerization by membrane-bound IRSp53, PIP(3), and Rac
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Cell Biol 173:571-85. 2006
  9. ncbi request reprint PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia
    Tsukasa Oikawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 6:420-6. 2004
  10. pmc Role of phospholipase C-L2, a novel phospholipase C-like protein that lacks lipase activity, in B-cell receptor signaling
    Kei Takenaka
    Department of Biochemistry, The Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Mol Cell Biol 23:7329-38. 2003

Detail Information

Publications82

  1. ncbi request reprint The WASP-WAVE protein network: connecting the membrane to the cytoskeleton
    Tadaomi Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokane dai, Minato ku, Tokyo 108 8639, Japan
    Nat Rev Mol Cell Biol 8:37-48. 2007
    ..Large families of membrane-binding proteins were recently found to interact with WASP and WAVE family proteins, therefore providing a new layer of membrane-dependent regulation of actin polymerization...
  2. pmc A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42
    M Fukuoka
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Cell Biol 152:471-82. 2001
    ..These findings suggest that WISH activates Arp2/3 complex through N-WASP-dependent and -independent pathways without Cdc42, resulting in the rapid actin polymerization required for microspike formation...
  3. ncbi request reprint Membrane targeting and remodeling through phosphoinositide-binding domains
    Tadaomi Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    IUBMB Life 58:296-303. 2006
    ....
  4. ncbi request reprint Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane
    T Takenawa
    Department of Biology, Institute of Medical Science, University of Tokyo, Japan
    Biochim Biophys Acta 1533:190-206. 2001
    ..Thus, through regulating actin cytoskeleton organization and membrane trafficking, phosphoinositides play crucial roles in a variety of cell functions such as growth, polarity, movement, and pattern formation...
  5. ncbi request reprint WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement
    T Takenawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Cell Sci 114:1801-9. 2001
    ..Thus, N-WASP, which is activated downstream of Cdc42 or independently by WISH, induces formation of filopodia and WAVE2, which is activated via IRSp53 downstream of Rac, induces formation of lamellipodia...
  6. ncbi request reprint N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor
    Kazuhiro Kawamura
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba 263 8522, Japan
    J Biol Chem 279:54862-71. 2004
    ..These results imply that both N-WASP and WAVE2, which are activated downstream of phosphati-dylinositol 3-kinase, are required for the migration through the lamellipodial formation of C2C12 cells induced by HGF...
  7. ncbi request reprint FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation
    Kiyohito Mizutani
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokane dai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 313:468-74. 2004
    ..These results indicate that FBP11 regulates localization of N-WASP, thus negatively regulating the function of N-WASP in the cytoplasm...
  8. pmc Optimization of WAVE2 complex-induced actin polymerization by membrane-bound IRSp53, PIP(3), and Rac
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    J Cell Biol 173:571-85. 2006
    ..Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac-dependent manner with PIP(3)-containing liposomes. Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP(3)...
  9. ncbi request reprint PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia
    Tsukasa Oikawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Nat Cell Biol 6:420-6. 2004
    ..These results suggest that one of the products of PI(3)K, PtdIns(3,4,5)P(3), recruits WAVE2 to the polarized membrane and that this recruitment is essential for lamellipodium formation at the leading edge...
  10. pmc Role of phospholipase C-L2, a novel phospholipase C-like protein that lacks lipase activity, in B-cell receptor signaling
    Kei Takenaka
    Department of Biochemistry, The Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Mol Cell Biol 23:7329-38. 2003
    ..These mice had a stronger T-cell-independent antigen response. These results indicate that PLC-L2 is a novel negative regulator of BCR signaling and immune responses...
  11. pmc Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis
    Kazuya Tsujita
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato ku, Tokyo, 108 8639 Japan
    J Cell Biol 172:269-79. 2006
    ..Furthermore, knockdown of endogenous FBP17 and CIP4 impaired endocytosis. Our data indicate that PCH protein family members couple membrane deformation to actin cytoskeleton reorganization in various cellular processes...
  12. doi request reprint Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation
    Kazunori Takano
    Department of Biology, Graduate School of Science, Chiba University, 1 33 Yayoicho, Inageku, Chiba 263 8522, Japan
    Science 330:1536-40. 2010
    ..These findings present the mechanisms of IGF-1-induced actin filament formation in myofibrillogenesis required for muscle maturation and hypertrophy and a mechanism of actin nucleation...
  13. ncbi request reprint Differential roles of WAVE1 and WAVE2 in dorsal and peripheral ruffle formation for fibroblast cell migration
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, 108 8639, Japan
    Dev Cell 5:595-609. 2003
    ..Thus, WAVE2 is essential for leading edge extension for directed migration in general and WAVE1 is essential in MMP-dependent migration in ECM...
  14. ncbi request reprint Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth
    Tomoyuki Abe
    Department of Biology, Faculty of Science, and Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Chiba, Chiba 263 8522, Japan
    J Cell Sci 116:155-68. 2003
    ..Consequently, N-WASP activated by these small GTPases mediates neuronal differentiation in addition to its recently identified role in glucose uptake...
  15. ncbi request reprint Rac-WAVE2 signaling is involved in the invasive and metastatic phenotypes of murine melanoma cells
    Shusaku Kurisu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Oncogene 24:1309-19. 2005
    ..Thus, WAVE2 acts as the primary effector downstream of Rac to achieve invasion and metastasis, suggesting that suppression of WAVE2 activity holds a promise for preventing cancer invasion and metastasis...
  16. ncbi request reprint The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery
    Koji Eto
    Laboratory of Stem Cell Therapy, Center for Experimental Medicine, The Institute of Medical Science, University of Tokyo, Japan
    Blood 110:3637-47. 2007
    ..These observations clarify indispensable roles for the WAVE2/Abi1 complex in alpha(IIb)beta(3)-mediated lamellipodia by MKs and platelets through Rac and IRSp53, and additionally in thrombopoiesis independent of Rac and IRSp53...
  17. pmc Interaction of HSP90 to N-WASP leads to activation and protection from proteasome-dependent degradation
    Sun Joo Park
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    EMBO J 24:1557-70. 2005
    ..These findings suggest that HSP90 induces efficient activation of N-WASP downstream of phosphorylation signal by Src family kinases and is critical for N-WASP-dependent podosome formation and neurite extension...
  18. ncbi request reprint Phospholipase Cdelta4: from genome structure to physiological function
    Kiyoko Fukami
    Department of Biochemistry, The Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8039, Japan
    Adv Enzyme Regul 43:87-106. 2003
  19. ncbi request reprint IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness
    Yosuke Funato
    Division of Cancer Genomics, Biochemistry, and Cancer Cell Research, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cancer Res 64:5237-44. 2004
    ..These findings implicate the importance of the IRSp53/Eps8 complex in Rac activation and metastatic behavior of the malignant tumor cells...
  20. pmc IRSp53 mediates podosome formation via VASP in NIH-Src cells
    Tsukasa Oikawa
    Laboratory of Cell and Tissue Biology, Keio University School of Medicine, Sinjuku, Tokyo, Japan
    PLoS ONE 8:e60528. 2013
    ..Interestingly, VASP physically interacted with IRSp53 in NIH-Src cells and was essential for podosome formation. These data highlight the role of IRSp53 as a linker of small GTPases to VASP for podosome formation...
  21. ncbi request reprint Application of phosphoinositide-binding domains for the detection and quantification of specific phosphoinositides
    Masahiro Furutani
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Anal Biochem 355:8-18. 2006
    ....
  22. ncbi request reprint Male-specific sterility caused by the loss of CR16
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Genes Cells 12:721-33. 2007
    ..The level of N-WASP but not the transcript was decreased in the testes and Sertoli cells of the CR16-knockout mice. Therefore, CR16 and N-WASP are suggested to play important roles in spermatogenesis...
  23. ncbi request reprint The RAC binding domain/IRSp53-MIM homology domain of IRSp53 induces RAC-dependent membrane deformation
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato ku, Tokyo 108 8639, USA
    J Biol Chem 281:35347-58. 2006
    ....
  24. ncbi request reprint Rac-WAVE-mediated actin reorganization is required for organization and maintenance of cell-cell adhesion
    Daisuke Yamazaki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Cell Sci 120:86-100. 2007
    ..Thus, Rac-WAVE-dependent actin reorganization is not only involved in formation of cell-cell adhesions but is also required for their maintenance...
  25. ncbi request reprint Thin layer chromatography-blotting, a novel method for the detection of phosphoinositides
    Masahiro Furutani
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639
    J Biochem 139:663-70. 2006
    ....
  26. ncbi request reprint Neural Wiskott-Aldrich syndrome protein is recruited to rafts and associates with endophilin A in response to epidermal growth factor
    Makiko Otsuki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Biol Chem 278:6461-9. 2003
    ..These results suggest that, upon EGF stimulation, N-WASP interacts with EA through its proline-rich domain to induce the fission step of clathrin-mediated endocytosis...
  27. ncbi request reprint Two verprolin homology domains increase the Arp2/3 complex-mediated actin polymerization activities of N-WASP and WAVE1 C-terminal regions
    Hideki Yamaguchi
    Division of Cancer Genomics, Institute of Medical Science, University of Tokyo, Japan
    Biochem Biophys Res Commun 297:214-9. 2002
    ..Thus, the WAVE1 VCA fragment may exist in an unfavorable conformation to activate the Arp2/3 complex, implying the existence of a structural difference between WAVE1 and N-WASP VCAs in addition to the number of V motifs...
  28. ncbi request reprint Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo
    Emi Tokuda
    Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan
    Cancer Res 67:9666-76. 2007
    ..These results indicate that CKIP-1, a novel Akt PH domain-interacting protein, would be a candidate of tumor suppressor with an Akt inhibitory function...
  29. ncbi request reprint Regulation of cancer cell motility through actin reorganization
    Daisuke Yamazaki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    Cancer Sci 96:379-86. 2005
    ..Inhibition of Rho family small GTPase signaling suppresses the migration and invasion of cancer cells. Thus, control of cell migration via the actin cytoskeleton provides the possibility of regulating cancer cell invasion and metastasis...
  30. pmc Regulation of actin cytoskeleton by mDab1 through N-WASP and ubiquitination of mDab1
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokane dai, Minato ku, Tokyo, 108 8639, Japan
    Biochem J 384:1-8. 2004
    ..These findings suggest that mDab1 regulates the actin cytoskeleton through N-WASP, which is negatively regulated by phosphorylation-mediated ubiquitination of mDab1...
  31. ncbi request reprint WAVE2 is required for directed cell migration and cardiovascular development
    Daisuke Yamazaki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Mianato ku, Tokyo 108 8639, Japan
    Nature 424:452-6. 2003
    ..These findings indicate that WAVE2-regulated actin reorganization might be required for proper cell movement and that a lack of functional WAVE2 impairs angiogenesis in vivo...
  32. pmc Phospholipase Cdelta1 is required for skin stem cell lineage commitment
    Yoshikazu Nakamura
    Department of Biochemistry, The Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    EMBO J 22:2981-91. 2003
    ..From these results, we conclude that PLCdelta(1) is required for skin stem cell lineage commitment...
  33. ncbi request reprint A novel function of WAVE in lamellipodia: WAVE1 is required for stabilization of lamellipodial protrusions during cell spreading
    Daisuke Yamazaki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Genes Cells 10:381-92. 2005
    ..Coordination of WAVE1 and WAVE2 activities appears to be necessary for formation of proper actin structures in stable lamellipodia...
  34. ncbi request reprint HSP90 cross-links branched actin filaments induced by N-WASP and the Arp2/3 complex
    Sun Joo Park
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108 8539, Japan 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8539, Japan
    Genes Cells 12:611-22. 2007
    ..These findings indicate that HSP90 promotes the formation of N-WASP/Arp2/3 complex-induced unbranched filopodial actin structures...
  35. pmc RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C
    Kazuhisa Koizumi
    Department of Biology, Graduate School of Science, Yayoicho, Inageku, Chiba 263 8522, Japan
    Mol Biol Cell 23:4647-61. 2012
    ..Knockdown of mDia3 obstructed RhoD-induced protrusion formation. These results imply that RhoD activated by FGF signaling forms cytoneme-like protrusions through activation of mDia3C, which induces actin filament formation...
  36. ncbi request reprint WICH, a member of WASP-interacting protein family, cross-links actin filaments
    Masayoshi Kato
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Mianato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 328:1058-66. 2005
    ..Taken together, these data suggest that WICH induces a bundled form of actin filament with actin cross-linking activity and the association with N-WASP suppresses that activity. WICH thus appears to be a novel actin bundling protein...
  37. ncbi request reprint Sustained activation of N-WASP through phosphorylation is essential for neurite extension
    Shiro Suetsugu
    Department of Biochemistry, 4 6 1 Shirokanedai, Minato ku, 108 8639, Tokyo, Japan
    Dev Cell 3:645-58. 2002
    ..Collectively, neurite extension is regulated by the balance of N-WASP phosphorylation (activation) and degradation (inactivation), which are induced by tyrosine phosphorylation...
  38. pmc DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras-ERK pathway, is required for myogenic differentiation
    Takashi Yokoyama
    Department of Biology, Graduate School of Science, Chiba University, Chiba 263 8522, Japan
    J Cell Biol 177:781-93. 2007
    ..These results imply that DA-Raf1 is the first identified competent, intrinsic, dominant-negative antagonist of the Ras-ERK pathway...
  39. ncbi request reprint Regulation of cortical actin networks in cell migration
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, and CREST, Japan Science and Technology Corporation, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Int Rev Cytol 229:245-86. 2003
    ..Through branching of actin filaments, Arp2/3 complex-dependent actin polymerization is suffcient to generate the force necessary for protrusion...
  40. ncbi request reprint Caenorhabditis elegans WASP-interacting protein homologue WIP-1 is involved in morphogenesis through maintenance of WSP-1 protein levels
    Mariko Sawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 340:709-17. 2006
    ..Our results suggest that C. elegans WIP-1 may function in hypodermal cell migration during ventral enclosure by maintaining levels of WSP-1...
  41. ncbi request reprint Cytoskeletal reorganization induced by insulin: involvement of Grb2/Ash, Ras and phosphatidylinositol 3-kinase signalling
    Kazuyuki Tobe
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Genes Cells 8:29-40. 2003
    ..We therefore investigated the involvement of Grb2/Ash-Ras and phosphatidylinositol (PI) 3-kinase in the insulin-induced morphological changes in fibroblasts over-expressing human insulin receptors (HIRcB cells)...
  42. ncbi request reprint Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosure
    Mariko Sawa
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Cell Sci 116:1505-18. 2003
    ..The stable localization of WSP-1 was dependent on the presence of Arp2/3 complex, suggesting an interaction between the Arp2/3 complex and WSP-1 in vivo...
  43. ncbi request reprint Molecular characterization of a novel RhoGAP, RRC-1 of the nematode Caenorhabditis elegans
    Mina Delawary
    Division of Oncology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 357:377-82. 2007
    ..These data contribute toward understanding the roles of Rho family GTPases in C. elegans...
  44. ncbi request reprint Translocation of N-WASP by nuclear localization and export signals into the nucleus modulates expression of HSP90
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Japan
    J Biol Chem 278:42515-23. 2003
    ..Because HSP90 is essential for the activities of Src family kinases, these results suggest that localization of N-WASP modulates Src kinase activity by regulating HSP90 expression...
  45. ncbi request reprint Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex
    Osamu Nakanishi
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokane dai, Tokyo 108 8639, Japan
    J Biochem 141:319-25. 2007
    ..However, the phosphorylation of the VCA region resulted in reduced induction of Arp2/3-mediated actin polymerization in vitro. The role of the phosphorylation of the proline-rich region was not determined...
  46. ncbi request reprint Spatial and temporal regulation of actin polymerization for cytoskeleton formation through Arp2/3 complex and WASP/WAVE proteins
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Cell Motil Cytoskeleton 51:113-22. 2002
  47. ncbi request reprint WAVE3 functions as a negative regulator of LDOC1
    Kiyohito Mizutani
    Department of Biochemistry, Institute of Medical Science, The University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639
    J Biochem 138:639-46. 2005
    ..Further, the expression of WAVE3 induces the translocation of LDOC1 from the nucleus to the cytoplasm, resulting in the inhibition of LDOC1-induced apoptosis. Thus, it is possible that the LDOC1 function is negatively regulated by WAVE3...
  48. ncbi request reprint Regulation of actin dynamics by WASP family proteins
    Hiroaki Miki
    Division of Cancer Genomics, Institute of Medical Science, University of Tokyo, Shirokanedai 4 6 1, Minato ku, Tokyo 108 8639
    J Biochem 134:309-13. 2003
    ..Analyses of model organisms have just begun to reveal unexpected functions of WASP family proteins in multicellular organisms...
  49. ncbi request reprint A positive role of phosphatidylinositol 3-kinase in aging phenotype expression in cultured human diploid fibroblasts
    Koozi Matuoka
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo, 108 8639, Japan
    Arch Gerontol Geriatr 36:203-19. 2003
    ....
  50. ncbi request reprint WAVE2 serves a functional partner of IRSp53 by regulating its interaction with Rac
    Hiroaki Miki
    Division of Cancer Genomics, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Biochem Biophys Res Commun 293:93-9. 2002
    ..We also report that IRSp53 induces spreading and neurite formation of N1E-115 cells, which presumably reflect functional cooperation with Rac...
  51. ncbi request reprint Essential role of neural Wiskott-Aldrich syndrome protein in podosome formation and degradation of extracellular matrix in src-transformed fibroblasts
    Kiyohito Mizutani
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Cancer Res 62:669-74. 2002
    ..These results suggest the importance of N-WASP in podosome formation and extracellular matrix degradation, which are processes thought to underlie the invasive phenotype of 3Y1-src cells...
  52. ncbi request reprint Small GTPase Rah/Rab34 is associated with membrane ruffles and macropinosomes and promotes macropinosome formation
    Peng Sun
    Department of Biology, Faculty of Science, and Graduate School of Science and Technology, Chiba University, Yayoicho, Inageku, Japan
    J Biol Chem 278:4063-71. 2003
    ..These results imply that Rah is required for efficient macropinosome formation from the membrane ruffles...
  53. ncbi request reprint Myotubularin regulates the function of the late endosome through the gram domain-phosphatidylinositol 3,5-bisphosphate interaction
    Kazuya Tsujita
    Department of Biochemistry, Institute of Medical Science, University of Tokyo and Japan Science and Technology Corporation, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Biol Chem 279:13817-24. 2004
    ..Thus, our data suggest that myotubularin phosphatase physiologically functions in late endosomal trafficking and vacuolar morphology through interaction with PtdIns(3,5)P(2)...
  54. ncbi request reprint From N-WASP to WAVE: key molecules for regulation of cortical actin organization
    Tadaomi Takenawa
    Institute of Medical Science, University of Tokyo and CREST, Japan Science and Technology Corporation, Japan
    Novartis Found Symp 269:3-10; discussion 10-5, 30-4 passim. 2005
    ..In this migration, WAVE1-deficient MEFs still could form lamellipodia but WAVE2-deficient MEFs could not. All these data show that WAVE2 but not WAVE1 is essential for lamellipodium formation and directed migration...
  55. pmc SKIP negatively regulates insulin-induced GLUT4 translocation and membrane ruffle formation
    Takeshi Ijuin
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Mol Cell Biol 23:1209-20. 2003
    ..SKIP also markedly inhibited GLUT4 translocation and membrane ruffle formation. We conclude that SKIP preferentially regulates glucose transport and actin cytoskeletal rearrangement among a variety of PI(3,4,5)P3 downstream events...
  56. ncbi request reprint [Regulation of actin polymerization by Rho family]
    Sunjoo Park
    Tanpakushitsu Kakusan Koso 51:511-5. 2006
  57. ncbi request reprint Phosphoinositide-binding domains: Functional units for temporal and spatial regulation of intracellular signalling
    Toshiki Itoh
    Department of Biochemistry, Institute of Medical Science, University of Tokyo and CREST, Japan Science and Technology Corporation, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan
    Cell Signal 14:733-43. 2002
    ....
  58. pmc Phospholipase Cdelta4 is required for Ca2+ mobilization essential for acrosome reaction in sperm
    Kiyoko Fukami
    Division of Biochemistry, The Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    J Cell Biol 161:79-88. 2003
    ..These results indicate that PLCdelta4 is an important enzyme for intracellular [Ca2+]i mobilization in the ZP-induced acrosome reaction and for sustained [Ca2+]i increases through SOC induced by ZP and progesterone in sperm...
  59. ncbi request reprint WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation
    Masayoshi Kato
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, 108 8639, Japan
    Biochem Biophys Res Commun 291:41-7. 2002
    ..In addition, expression of the W fragment of WICH suppresses microspike formation induced by N-WASP, indicating an essential role for WICH in N-WASP-induced microspike formation...
  60. ncbi request reprint Stress-associated MAP kinase fills in the map of filamin-mediated neuronal migration
    Shiro Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokane dai, Tokyo 108 8639, Japan
    Dev Cell 12:3-4. 2007
  61. ncbi request reprint Neural Wiskott-Aldrich syndrome protein is involved in hepatocyte growth factor-induced migration, invasion, and tubulogenesis of epithelial cells
    Hideki Yamaguchi
    Division of Biochemistry, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Cancer Res 62:2503-9. 2002
    ..These results indicate that N-WASP regulates HGF-induced cell migration and invasion, which are required for epithelial tubulogenesis...
  62. ncbi request reprint Actin -related protein 3 (Arp3) is mutated in proteinuric BUF/Mna rats
    Kiyotaka Akiyama
    Central Pharmaceutical Research Institute, Japan Tobacco Inc, Yokohama, 236 0004, Japan
    Mamm Genome 19:41-50. 2008
    ..Taken together, these data suggest that Arp3 is a candidate for the Pur1 gene. This observation is consistent with our growing recognition that abnormal signaling-induced assembly of actin in podocytes leads to the development of FSGS...
  63. ncbi request reprint The rat homologue of Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) associates with actin filaments, recruits N-WASP from the nucleus, and mediates mobilization of actin from stress fibers in favor of filopodia formation
    Susanne Vetterkind
    Institute of Genetics, University of Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
    J Biol Chem 277:87-95. 2002
    ..Interaction of N-WASP with WIP may in turn lead to mobilization of actin from stress fibers and nucleation of new actin filaments in filopodia...
  64. ncbi request reprint [Phosphatidylinositol in signal transduction]
    Tadaomi Takenawa
    Tanpakushitsu Kakusan Koso 52:1378-86. 2007
  65. ncbi request reprint SGIP1alpha is an endocytic protein that directly interacts with phospholipids and Eps15
    Akiyoshi Uezu
    Department of Molecular Pharmacology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    J Biol Chem 282:26481-9. 2007
    ..These results suggest that SGIP1alpha plays an essential role in clathrin-mediated endocytosis by interacting with phospholipids and Eps15...
  66. pmc Synaptopodin protects against proteinuria by disrupting Cdc42:IRSp53:Mena signaling complexes in kidney podocytes
    Etsuko Yanagida-Asanuma
    Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Am J Pathol 171:415-27. 2007
    ..The identification of synaptopodin as an inhibitor of Cdc42:IRSp53:Mena signaling defines a novel antiproteinuric signaling pathway and offers new targets for the development of antiproteinuric therapeutic modalities...
  67. pmc Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin
    Hideki Yamaguchi
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 168:441-52. 2005
    ..These results indicate that EGF receptor signaling regulates invadopodium formation through the N-WASP-Arp2/3 pathway and cofilin is necessary for the stabilization and maturation of invadopodia...
  68. pmc CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation
    Yoji Kawano
    Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Mol Cell Biol 25:9920-35. 2005
    ..These results suggest that CRMP-2 transports the Sra-1/WAVE1 complex to axons in a kinesin-1-dependent manner and thereby regulates axon outgrowth and formation...
  69. ncbi request reprint Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone
    Motohiro Nozumi
    Department of Biochemical Science, Kyushu Institute of Technology, 680 4 Kawatsu, lizuka, Fukuoka 820 8502, Japan
    J Cell Sci 116:239-46. 2003
    ..These results indicate that WAVE2 or WAVE3 may guide the actin bundles into the filopodia and promote actin assembly at the tips...
  70. ncbi request reprint Arp2/3 activity is necessary for efficient formation of E-cadherin adhesive contacts
    Suzie Verma
    Institute for Molecular Bioscience, School for Biomedical Science, The University of Queensland, St Lucia, Queensland 4072, Australia
    J Biol Chem 279:34062-70. 2004
    ..These findings demonstrate that Arp2/3 activity is necessary for cells to efficiently extend and assemble cadherin-based adhesive contacts...
  71. pmc Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development
    Yoshikazu Nakamura
    Laboratory of Genome and Biosignal, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Mol Cell Biol 25:10979-88. 2005
    ..5, clearly indicating that the embryonic lethality is caused by a defect in trophoblasts. On the basis of these results, we conclude that PLCdelta1 and PLCdelta3 are essential in trophoblasts for placental development...
  72. ncbi request reprint Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs
    Zen Kouchi
    Department of Physiology, Tokyo Women s Medical University School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    J Biol Chem 279:10408-12. 2004
    ....
  73. ncbi request reprint Disruption of phospholipase Cdelta4 gene modulates the liver regeneration in cooperation with nuclear protein kinase C
    Atsushi Akutagawa
    Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, 65, Tsurumai cho, Showa Ku, Nagoya 466 8550
    J Biochem 140:619-25. 2006
    ..PKC epsilon also increased transiently in chromatin from PLC delta4(+/+) at late S. It is concluded that PLC delta4 regulates the liver regeneration in cooperation with nuclear PKC alpha and epsilon...
  74. ncbi request reprint Modulation of GABA(A) receptor phosphorylation and membrane trafficking by phospholipase C-related inactive protein/protein phosphatase 1 and 2A signaling complex underlying brain-derived neurotrophic factor-dependent regulation of GABAergic inhibition
    Takashi Kanematsu
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 281:22180-9. 2006
    ..Together, the results suggest that PRIP plays an important role in BDNF-dependent regulation of GABA(A) receptors by mediating the specific association between beta subunits of these receptors with protein phosphatases...
  75. ncbi request reprint IRSp53 is colocalised with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena
    Hiroyuki Nakagawa
    Department of Cell Biology, Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, Salzburg A 5020, Austria
    J Cell Sci 116:2577-83. 2003
    ..These results suggest an alternative modulatory role for IRSp53 in the extension of both filopodia and lamellipodia, through WAVE2...
  76. ncbi request reprint Evidence that activation of Src family kinase is not required for fertilization-associated [Ca2+]i oscillations in mouse eggs
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Reproduction 127:441-54. 2004
    ..We can therefore conclude that activation of an SFK is neither necessary nor sufficient for triggering fertilization-induced [Ca2+]i oscillations...
  77. ncbi request reprint WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor
    Helene Bierne
    Unité des Interactions Bactéries Cellules, Institut Pasteur, INSERM U604, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Cell Sci 118:1537-47. 2005
    ....
  78. ncbi request reprint WAVE/Scars in platelets
    Atsushi Oda
    Laboratory of Environmental Biology, Department of Preventive Medicine, Hokkaido University School of Medicine, Kitaku, Sapporo, 060 8638, Japan
    Blood 105:3141-8. 2005
    ..These data suggest that the 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of actin cytoskeleton in platelets...
  79. ncbi request reprint Phospholipase C-related inactive protein is involved in trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface
    Akiko Mizokami
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, and Station for Collaborative Research, Kyushu University, Fukuoka 812 8582, Japan
    J Neurosci 27:1692-701. 2007
    ..These results suggest that PRIP is implicated in the trafficking of gamma2 subunit-containing GABA(A) receptors to the cell surface, probably by acting as a bridging molecule between GABARAP and the receptors...
  80. ncbi request reprint Protein kinase A-dependent increase in WAVE2 expression induced by the focal adhesion protein vinexin
    Masaru Mitsushima
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Genes Cells 11:281-92. 2006
    ..These results suggest that vinexin beta regulates the proteasome-dependent degradation of WAVE2 in a PKA-dependent manner...
  81. ncbi request reprint Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis
    Atsushi Shimada
    RIKEN SPring 8 Center, Harima Institute, 1 1 1 Kouto, Sayo, Hyogo 679 5148, Japan
    Cell 129:761-72. 2007
    ..Furthermore, FBP17 is recruited to clathrin-coated pits in the late stage of CME, indicating its physiological role...
  82. ncbi request reprint Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex
    Diego Sbrissa
    Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Biol Chem 282:23878-91. 2007
    ..We suggest that the tight regulation in PtdIns(3,5)P2 homeostasis is mechanistically linked to early endosome dynamics in the course of cargo transport...