Tsutomu Suzuki

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. pmc Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
    Satoshi Kimura
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bldg 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 38:1341-52. 2010
  2. pmc Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity
    Satoshi Kimura
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 40:4071-85. 2012
  3. pmc Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic-binding sequence for ribosomal protein L2
    Kei Kitahara
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:4018-29. 2007
  4. pmc Specific residues at every third position of siRNA shape its efficient RNAi activity
    Takayuki Katoh
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:e27. 2007
  5. pmc Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method
    Kenjyo Miyauchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, Graduate School of Frontier Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:e24. 2007
  6. pmc LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria
    Takeshi Chujo
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Nucleic Acids Res 40:8033-47. 2012
  7. pmc Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile
    Takaaki Taniguchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 41:2621-31. 2013
  8. ncbi request reprint [Mass spectrometry reveals functional aspects of non-coding RNAs]
    Tsutomu Suzuki
    Tanpakushitsu Kakusan Koso 51:2431-5. 2006
  9. doi request reprint Human mitochondrial diseases caused by lack of taurine modification in mitochondrial tRNAs
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Wiley Interdiscip Rev RNA 2:376-86. 2011
  10. doi request reprint Discovery and characterization of tRNAIle lysidine synthetase (TilS)
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    FEBS Lett 584:272-7. 2010

Detail Information

Publications86

  1. pmc Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
    Satoshi Kimura
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bldg 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 38:1341-52. 2010
    ..These results suggest that m(4)Cm1402 plays a role in fine-tuning the shape and function of the P-site, thus increasing decoding fidelity...
  2. pmc Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity
    Satoshi Kimura
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 40:4071-85. 2012
    ..In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD...
  3. pmc Functional genetic selection of Helix 66 in Escherichia coli 23S rRNA identified the eukaryotic-binding sequence for ribosomal protein L2
    Kei Kitahara
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:4018-29. 2007
    ....
  4. pmc Specific residues at every third position of siRNA shape its efficient RNAi activity
    Takayuki Katoh
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:e27. 2007
    ..As an algorithm ('siExplorer') developed by this observation successfully calculated the activities of siRNAs targeting endogenous human genes, the 3-nt periodicity provides a new aspect unveiling siRNA functionality...
  5. pmc Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method
    Kenjyo Miyauchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, Graduate School of Frontier Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 35:e24. 2007
    ..RCC employs multiple tip-columns packed with solid-phase DNA probes to isolate multiple RNA species from a common sample of total RNAs. A pilot RCC instrument successfully isolated various ncRNAs from E. coli, yeast and mouse...
  6. pmc LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria
    Takeshi Chujo
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Nucleic Acids Res 40:8033-47. 2012
    ..Moreover, LRPPRC promoted the polyadenylation of mRNAs mediated by mitochondrial poly(A) polymerase (MTPAP) in vitro. These findings provide a framework for understanding the molecular mechanism of mRNA metabolism in human mitochondria...
  7. pmc Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile
    Takaaki Taniguchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 41:2621-31. 2013
    ..mobile ribosome has a property that prevents misreading of AUG codon. These findings provide an insight into the evolutionary reorganization of the AUA decoding system...
  8. ncbi request reprint [Mass spectrometry reveals functional aspects of non-coding RNAs]
    Tsutomu Suzuki
    Tanpakushitsu Kakusan Koso 51:2431-5. 2006
  9. doi request reprint Human mitochondrial diseases caused by lack of taurine modification in mitochondrial tRNAs
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Wiley Interdiscip Rev RNA 2:376-86. 2011
    ..These findings represent the first evidence of a molecular pathogenesis caused by an RNA modification disorder...
  10. doi request reprint Discovery and characterization of tRNAIle lysidine synthetase (TilS)
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    FEBS Lett 584:272-7. 2010
    ..Recent structural studies unveiled tRNA recognition by TilS, and a molecular basis of lysidine formation at atomic resolution...
  11. doi request reprint Human mitochondrial tRNAs: biogenesis, function, structural aspects, and diseases
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo 113 8656, Japan
    Annu Rev Genet 45:299-329. 2011
    ..These nuclear factors are also targets of pathogenic mutations linked to various diseases, indicating the functional importance of mt tRNAs for mitochondrial activity...
  12. ncbi request reprint Chronic pain-induced emotional dysfunction is associated with astrogliosis due to cortical delta-opioid receptor dysfunction
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    J Neurochem 97:1369-78. 2006
    ..This phenomenon may lead to emotional disorders including aggravated anxiety under chronic pain-like state...
  13. ncbi request reprint Direct evidence of astrocytic modulation in the development of rewarding effects induced by drugs of abuse
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Neuropsychopharmacology 31:2476-88. 2006
    ..The present study provides direct evidence that astrocytes may, at least in part, contribute to the synaptic plasticity induced by drugs of abuse during the development of rewarding effects induced by psychostimulants and opioids...
  14. ncbi request reprint Comparative pharmacological profiles of morphine and oxycodone under a neuropathic pain-like state in mice: evidence for less sensitivity to morphine
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    Neuropsychopharmacology 33:1097-112. 2008
    ..Furthermore, the reduction in G-protein activation induced by M-6-G may, at least in part, contribute to the suppression of the antinociceptive effect produced by morphine under a neuropathic pain-like state...
  15. doi request reprint Implication of activated astrocytes in the development of drug dependence: differences between methamphetamine and morphine
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Ann N Y Acad Sci 1141:96-104. 2008
    ..These findings provide direct evidence that astrocytes may, at least in part, contribute to the development of the rewarding effects induced by drugs of abuse in the nucleus accumbens and cingulate cortex...
  16. pmc Biogenesis of glutaminyl-mt tRNAGln in human mitochondria
    Asuteka Nagao
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 106:16209-14. 2009
    ....
  17. ncbi request reprint Chronic pain induces anxiety with concomitant changes in opioidergic function in the amygdala
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    Neuropsychopharmacology 31:739-50. 2006
    ..Collectively, the present data constitute the first evidence that chronic pain has an anxiogenic effect in mice. This phenomenon may be associated with changes in opioidergic function in the amygdala...
  18. doi request reprint Direct evidence for the ongoing brain activation by enhanced dynorphinergic system in the spinal cord under inflammatory noxious stimuli
    Yasuko Taketa
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    Anesthesiology 112:418-31. 2010
    ..In this study, the authors investigated the role of spinal dynorphinergic transmission in ongoing brain activation under noxious stimuli in mice...
  19. ncbi request reprint Implication of cyclin-dependent kinase 5 in the development of psychological dependence on and behavioral sensitization to morphine
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    J Neurochem 93:1463-8. 2005
    ..These findings suggest that the induction and/or activation of cdk5 are implicated in the development of psychological dependence on morphine...
  20. ncbi request reprint Role of delta-opioid receptor function in neurogenesis and neuroprotection
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo, 142 8501, Japan
    J Neurochem 97:1494-505. 2006
    ..These findings raise the possibility that delta-opioid receptors play a crucial role in neurogenesis and neuroprotection, mainly through the activation of Trk-dependent tyrosine kinase, which could be linked to PI3K, PKC, CaMKII and MEK...
  21. ncbi request reprint Changes in central dopaminergic systems and morphine reward by prenatal and neonatal exposure to bisphenol-A in mice: evidence for the importance of exposure period
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawaku, Tokyo, Japan
    Addict Biol 12:167-72. 2007
    ..In conclusion, the present data suggest that the organogenesis and lactation are the most important period to cause the alternation of dopaminergic system by bisphenol-A exposure in the mouse...
  22. ncbi request reprint Direct evidence for spinal cord microglia in the development of a neuropathic pain-like state in mice
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    J Neurochem 97:1337-48. 2006
    ....
  23. doi request reprint Post-synaptic action of morphine on glutamatergic neuronal transmission related to the descending antinociceptive pathway in the rat thalamus
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Ebara, Shinagawa ku, Tokyo, Japan
    J Neurochem 104:469-78. 2008
    ..The present data suggest that the stimulation of MOR in the Hb may be involved in activation of the descending antinociceptive pathway through glutamatergic neurotransmission via the NMDA receptor...
  24. pmc Decoding mechanism of non-universal genetic codes in Loligo bleekeri mitochondria
    Takayuki Ohira
    From the Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 288:7645-52. 2013
    ..In addition, 5-taurinomethyl-2-thiouridine (τm(5)s(2)U) was found in mitochondrial tRNAs for Leu(UUR) and Lys in L. bleekeri. This is the first discovery of τm(5)U and τm(5)s(2)U in molluscan mitochondrial tRNAs...
  25. ncbi request reprint Direct evidence for the involvement of the mesolimbic kappa-opioid system in the morphine-induced rewarding effect under an inflammatory pain-like state
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Ebara, Shinagawa ku, Tokyo, Japan
    Neuropsychopharmacology 30:111-8. 2005
    ..Acc., resulting in suppression of the morphine-induced rewarding effect in rats. The present study provides further evidence of the clinical usefulness of morphine in patients suffering from severe pain...
  26. doi request reprint Usefulness of olanzapine as an adjunct to opioid treatment and for the treatment of neuropathic pain
    Kazuhiro Torigoe
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Anesthesiology 116:159-69. 2012
    ..However, chronic pain can induce sleep disturbance. The authors investigated the effects of the atypical antipsychotic olanzapine on morphine-induced emesis and the sleep dysregulation associated with chronic pain...
  27. ncbi request reprint Role of spinal voltage-dependent calcium channel alpha 2 delta-1 subunit in the expression of a neuropathic pain-like state in mice
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo, 142 8501, Japan
    Life Sci 80:2015-24. 2007
    ..Furthermore, the present data provide evidence that the neuropathic pain may be effectively controlled by repeated treatment with GBP at the early stage...
  28. ncbi request reprint Long-lasting change in brain dynamics induced by methamphetamine: enhancement of protein kinase C-dependent astrocytic response and behavioral sensitization
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Ebara, Tokyo, Japan
    J Neurochem 93:1383-92. 2005
    ..This information can break through the definition of drugs of abuse and the misleading of concept that morphine produces a long-lasting neurotoxicity...
  29. ncbi request reprint The pathogenic A4269G mutation in human mitochondrial tRNA(Ile) alters the T-stem structure and decreases the binding affinity for elongation factor Tu
    Narumi Hino
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa shi, Chiba 277 8562, Japan
    Genes Cells 9:243-52. 2004
    ..This observation provides insight into the molecular pathology associated with tRNA dysfunction caused by this pathogenic point mutation...
  30. ncbi request reprint Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases
    Noriko Umeda
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan
    J Biol Chem 280:1613-24. 2005
    ..Partial inactivation of MTU1 in HeLa cells by small interference RNA also reduced their oxygen consumption and resulted in mitochondria with defective membrane potentials, which are similar phenotypic features observed in MERRF...
  31. ncbi request reprint Oxidative stress in methamphetamine-induced self-injurious behavior in mice
    Tomohisa Mori
    Department of Legal Medicine, Tokyo Women s Medical University, Tokyo, Japan
    Behav Pharmacol 18:239-49. 2007
    ..These findings may help us to better understand the clinical phenomenon of self-injurious behavior...
  32. ncbi request reprint Role of Src family kinase in the rewarding effect and hyperlocomotion induced by morphine
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Neuroreport 17:115-9. 2006
    ..These findings suggest that Src family kinases in the nucleus accumbens are involved in the rewarding effect and hyperlocomotion induced by morphine...
  33. doi request reprint Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U.G wobble pairing during decoding
    Shinya Kurata
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 283:18801-11. 2008
    ..G base pair does not have classical U.G wobble geometry. These structures provide help to explain how the taum(5)U modification enables efficient decoding of UUG codons...
  34. doi request reprint Suppression of dopamine-related side effects of morphine by aripiprazole, a dopamine system stabilizer
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Eur J Pharmacol 600:105-9. 2008
    ..These results suggest that the co-administration of aripiprazole may be useful for reducing the severity of morphine-induced dopamine-related side effects...
  35. ncbi request reprint Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions
    Yoshiho Ikeuchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Mol Cell 21:97-108. 2006
    ....
  36. ncbi request reprint molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition
    Yoshiho Ikeuchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo ku, Tokyo 113 8656, Japan
    Mol Cell 19:235-46. 2005
    ..These findings reveal a dedicated mechanism embedded in tRNA(Ile) that controls its recognition and discrimination by TilS, and indicate the significance of this enzyme in the proper deciphering of genetic information...
  37. ncbi request reprint Inhibition of the morphine-induced rewarding effect by direct activation of spinal protein kinase C in mice
    Kousei Oe
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo, 142 8501, Japan
    Psychopharmacology (Berl) 177:55-60. 2004
    ..However, little direct evidence is available for the involvement of activated PKC in the spinal cord in reduction of rewarding effects induced by morphine under chronic pain-like state...
  38. ncbi request reprint Acquisition of the wobble modification in mitochondrial tRNALeu(CUN) bearing the G12300A mutation suppresses the MELAS molecular defect
    Yohei Kirino
    Department of Chemistry and Biotechnology, Graduate School of Engineering, Graduate School of Frontier Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Hum Mol Genet 15:897-904. 2006
    ....
  39. doi request reprint A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification
    Kenjyo Miyauchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan
    Nat Chem Biol 9:105-11. 2013
    ..ct(6)A was involved in promoting decoding efficiency. Structural modeling suggests that ct(6)A recognizes the first adenine base of ANN codon at the ribosomal A site...
  40. doi request reprint Enhancement of glutamatergic transmission in the cingulate cortex in response to mild noxious stimuli under a neuropathic pain-like state
    Keiichi Niikura
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Ebara, Shinagawa ku, Tokyo, Japan
    Synapse 65:424-32. 2011
    ..These findings raise the possibility that mild noxious heat stimuli under a peripheral nerve injury may increase the release of glutamate and promote its related postneuronal activity in the CG...
  41. pmc Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions
    Akiko Noma
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bldg 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 37:1335-52. 2009
    ..The sulfur-flow of eukaryotic 2-thiouridine formation is distinct mechanism from the bacterial sulfur-relay system which is based on the persulfide chemistry...
  42. doi request reprint Implication of dopaminergic projection from the ventral tegmental area to the anterior cingulate cortex in μ-opioid-induced place preference
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Addict Biol 15:434-47. 2010
    ..The activation of DARPP32 and CREB through dopamine D1 receptors in the ACG could be implicated in the maintenance of μ-opioid-induced place preference...
  43. ncbi request reprint Molecular mechanism of changes in the morphine-induced pharmacological actions under chronic pain-like state: suppression of dopaminergic transmission in the brain
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo, 142 8501, Japan
    Life Sci 74:2655-73. 2004
    ..Conclusively, our recent findings provide novel evidences for the mechanism underlying the less sensitivity to opioids under a neuropathic pain-like state...
  44. pmc The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon
    Yoshiho Ikeuchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 27:2194-203. 2008
    ..Our findings reveal an elaborate mechanism embedded in tRNA(Met) and tRNA(Ile) for the accurate decoding of AUA/AUG codons on the basis of the recognition of wobble bases by the respective RNA-modifying enzymes...
  45. ncbi request reprint Involvement of spinal metabotropic glutamate receptor 5 in the development of tolerance to morphine-induced antinociception
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    J Neurochem 94:1297-305. 2005
    ....
  46. pmc Crystal structure of a novel JmjC-domain-containing protein, TYW5, involved in tRNA modification
    Megumi Kato
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 2 11 16 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Nucleic Acids Res 39:1576-85. 2011
    ..These findings extend the repertoire of the tRNA modification enzyme into the Fe(II)/2-OG oxygenase superfamily...
  47. ncbi request reprint Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase
    Takashi Nagaike
    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba Prefecture, Japan
    J Biol Chem 280:19721-7. 2005
    ..These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs...
  48. ncbi request reprint The 3' termini of mouse Piwi-interacting RNAs are 2'-O-methylated
    Tomoya Ohara
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 8656, Japan
    Nat Struct Mol Biol 14:349-50. 2007
    ..Mass spectrometric analysis reveals that the piRNAs tested are fully modified by 2'-O-methylation at their 3' termini. This observation may provide a clue to the biogenesis and function of piRNAs in spermatogenesis...
  49. pmc Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease
    Yohei Kirino
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 101:15070-5. 2004
    ..We thus concluded that the UUG codon-specific translational defect of the mutant mt tRNAs(Leu(UUR)) is the primary cause of MELAS at the molecular level. This result could explain the complex I deficiency observed clinically in MELAS...
  50. pmc Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs
    Takeshi Chujo
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    RNA 18:2269-76. 2012
    ..The bacterial origin of Trmt61B is supported by the results of the phylogenetic analysis...
  51. ncbi request reprint The A-site finger in 23 S rRNA acts as a functional attenuator for translocation
    Taeko Komoda
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 281:32303-9. 2006
    ....
  52. pmc Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae
    Akiko Noma
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo ku, Tokyo 113 8656, Japan
    RNA 17:1111-9. 2011
    ..Thus, ABP140 has been designated TRM140 according to the preferred nomenclature. In addition, we observed a specific reduction of m³C formation in HeLa cells by siRNA-mediated knock down of the human ortholog of TRM140...
  53. ncbi request reprint Role of neuronal NR2B subunit-containing NMDA receptor-mediated Ca2+ influx and astrocytic activation in cultured mouse cortical neurons and astrocytes
    Hideaki Kato
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    Synapse 59:10-7. 2006
    ..Thus, glutamate-mediated pathway via NR2B subunit-containing NMDA receptor may, at least in part, contribute to neuron-to-astrocyte signaling...
  54. ncbi request reprint [Effects of fasudil on neuropathic pain-like state in mice]
    Mitsuru Shiokawa
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Nihon Shinkei Seishin Yakurigaku Zasshi 27:153-9. 2007
    ..The present data raise the fascinating possibility that i.t. or epidural administration with fasudil may be useful for the treatment of neuropathic pain...
  55. doi request reprint Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea
    Yoshiho Ikeuchi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Nat Chem Biol 6:277-82. 2010
    ..Although agm(2)C is chemically similar to lysidine, TiaS constitutes a distinct class of enzyme from tRNA(Ile)-lysidine synthetase (TilS), suggesting that the decoding systems evolved convergently across domains...
  56. ncbi request reprint Functional compatibility of elongation factors between mammalian mitochondrial and bacterial ribosomes: characterization of GTPase activity and translation elongation by hybrid ribosomes bearing heterologous L7/12 proteins
    Maki Terasaki
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg FSB 301, 5 1 5 Kashiwanoha, Kashiwa, 277 8562, Chiba Prefecture, Japan
    J Mol Biol 336:331-42. 2004
    ..These results demonstrate that functional compatibility between elongation factors and the L7/12 protein in the ribosome governs its translational specificity...
  57. pmc Taurine as a constituent of mitochondrial tRNAs: new insights into the functions of taurine and human mitochondrial diseases
    Takeo Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Building FSB 301, 5 1 5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277 8562, Japan
    EMBO J 21:6581-9. 2002
    ....
  58. pmc Ribosomal RNAs are tolerant toward genetic insertions: evolutionary origin of the expansion segments
    Takeshi Yokoyama
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 36:3539-51. 2008
    ..Most of the insertion sites show clear tendency to coincide with the regions of the expansion segments found in eukaryotic rRNAs, implying that eukaryotic rRNAs evolved from prokaryotic rRNAs suffering genetic insertions and selections...
  59. ncbi request reprint Rewarding effects of ethanol combined with low doses of morphine through dopamine D1 receptors
    Yuya Ise
    Section of Pharmaceutical Services, Nippon Medical School Hospital, Nippon Medical School, Tokyo, Japan
    J Nippon Med Sch 80:34-41. 2013
    ..0 mg/kg/day, s.c.). These results suggest that the rewarding effect induced by ethanol and a low dose of morphine is mediated by activation of the central opioidergic and dopaminergic systems through dopamine D1 receptors...
  60. pmc Dual-mode recognition of noncanonical tRNAs(Ser) by seryl-tRNA synthetase in mammalian mitochondria
    Sarin Chimnaronk
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba, Japan
    EMBO J 24:3369-79. 2005
    ..Our data further uncover an unprecedented mechanism of a dual-mode recognition employed to discriminate two distinct 'bizarre' mt tRNAs(Ser) by alternative combination of interaction sites...
  61. pmc Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria
    Takeo Suzuki
    Department of Chemistry and Biotechnology, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 286:35494-8. 2011
    ..Acquisition of the wobble modification appears to be a prerequisite for the genetic code alteration...
  62. ncbi request reprint [Role of astrocytes in rewarding effects of drugs of abuse]
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Nihon Shinkei Seishin Yakurigaku Zasshi 26:33-9. 2006
    ....
  63. doi request reprint Effects of dronabinol on morphine-induced dopamine-related behavioral effects in animals
    Tomohisa Mori
    Department of Toxicology, School of Pharmacy, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Synapse 66:931-7. 2012
    ....
  64. ncbi request reprint Human mitochondrial diseases associated with tRNA wobble modification deficiency
    Yohei Kirino
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 8565, Japan
    RNA Biol 2:41-4. 2005
    ..These findings suggest that the wobble modification deficiency plays a primary role in the molecular pathogenesis of the MELAS and MERRF mitochondrial diseases...
  65. pmc Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center
    Neuza Satomi Sato
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 103:15386-91. 2006
    ....
  66. ncbi request reprint Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases
    Takehiro Yasukawa
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan
    FEBS Lett 579:2948-52. 2005
    ..These findings demonstrate the generality of the wobble modification deficiency in mutant tRNAs in MELAS and MERRF...
  67. ncbi request reprint Antibiotic susceptibility of mammalian mitochondrial translation
    Li Zhang
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    FEBS Lett 579:6423-7. 2005
    ..coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals...
  68. ncbi request reprint Chronic morphine treatment increases the expression of vesicular glutamate transporter 1 in the mouse spinal cord
    Masami Suzuki
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, 142 8501, Japan
    Eur J Pharmacol 535:166-8. 2006
    ..These findings suggest that repeated treatment with morphine enhances excitatory synaptic transmission in the spinal cord, and in turn suppresses the morphine-induced antinociception...
  69. pmc Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2
    Takayuki Katoh
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo ku, Tokyo 113 8656, Japan
    Genes Dev 23:433-8. 2009
    ....
  70. pmc Specific correlation between the wobble modification deficiency in mutant tRNAs and the clinical features of a human mitochondrial disease
    Yohei Kirino
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 102:7127-32. 2005
    ....
  71. pmc Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA
    Akiko Noma
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 25:2142-54. 2006
    ..This hypothesis is also supported by the fact that plant ortholog is a large fusion protein consisting of TYW2 and TYW3 with the C-terminal domain of TYW4...
  72. pmc Common thiolation mechanism in the biosynthesis of tRNA thiouridine and sulphur-containing cofactors
    Naoki Shigi
    Biomedicinal Information Research Center BIRC, National Institute of Advanced Industrial Science and Technology AIST, Tokyo, Japan
    EMBO J 27:3267-78. 2008
    ..The results are discussed in relation to the mechanism and evolution of the eukaryotic ubiquitin system...
  73. ncbi request reprint Mass spectrometric identification and characterization of RNA-modifying enzymes
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Methods Enzymol 425:211-29. 2007
    ....
  74. ncbi request reprint The ordered transcription of RNA domains is not essential for ribosome biogenesis in Escherichia coli
    Kei Kitahara
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo 113 8656, Japan
    Mol Cell 34:760-6. 2009
    ..However, severe growth defects of all CPs were found in the absence of the DEAD box RNA helicase deaD, indicating that DeaD assists in the efficient assembly of each subunit in the cell...
  75. doi request reprint Hippocampal epigenetic modification at the brain-derived neurotrophic factor gene induced by an enriched environment
    Naoko Kuzumaki
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa ku, Tokyo, Japan
    Hippocampus 21:127-32. 2011
    ..These results suggest that an enriched environment increases BDNF mRNA expression via sustained epigenetic modification in the mouse hippocampus...
  76. ncbi request reprint [Behavioral analysis of chronic exposure to diphenylarsinic and associated influence on central nervous systems]
    Kazuya Miyagawa
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Nihon Shinkei Seishin Yakurigaku Zasshi 27:181-9. 2007
    ..Moreover, neonatal exposure to DPAA leads to the irreversible motor impairment associated with abnormalities in the cerebellum...
  77. ncbi request reprint [The world of RNA modifications; another topics of functional RNAs]
    Tsutomu Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Seikagaku 77:1481-96. 2005
  78. pmc Wobble modification differences and subcellular localization of tRNAs in Leishmania tarentolae: implication for tRNA sorting mechanism
    Tomonori Kaneko
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Building FSB 301, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    EMBO J 22:657-67. 2003
    ....
  79. ncbi request reprint [Recent topics in mitochondrial translation systems]
    Kimitsuna Watanabe
    Tanpakushitsu Kakusan Koso 48:365-74. 2003
  80. ncbi request reprint [Subcellular localization of RNA-modifying enzymes and maturation mechanism of RNA]
    Akiko Noma
    Tanpakushitsu Kakusan Koso 51:2226-31. 2006
  81. ncbi request reprint Conserved loop sequence of helix 69 in Escherichia coli 23 S rRNA is involved in A-site tRNA binding and translational fidelity
    Naomi Hirabayashi
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 281:17203-11. 2006
    ..This study proposes that H69 plays an important role in the control of translational fidelity by modulating A-site tRNA binding during the decoding process...
  82. ncbi request reprint Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase
    Sarin Chimnaronk
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Acta Crystallogr D Biol Crystallogr 60:1319-22. 2004
    ..89, b = 230.42, c = 135.60 A. There is one dimer (M(r) approximately 113 kDa) in the asymmetric unit, with a solvent content of 55%. Efforts to solve the phase problem by molecular replacement are under way...
  83. ncbi request reprint Decreased CCA-addition in human mitochondrial tRNAs bearing a pathogenic A4317G or A10044G mutation
    Yukihide Tomari
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba Prefecture 277 8562, Japan
    J Biol Chem 278:16828-33. 2003
    ..These are the first reported instances of inefficient CCA-addition being one of the apparent molecular pathogeneses caused by pathogenic point mutations in human mitochondrial tRNA genes...
  84. doi request reprint Measuring mRNA decay in human mitochondria
    Asuteka Nagao
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan
    Methods Enzymol 447:489-99. 2008
    ..In this report, we describe a practical method for measuring the half-lives of human mitochondrial mRNAs using quantitative real-time reverse transcription PCR...
  85. ncbi request reprint Chaplet column chromatography: isolation of a large set of individual RNAs in a single step
    Takeo Suzuki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Methods Enzymol 425:231-9. 2007
    ..quot; We successfully isolated all species of mammalian mitochondrial tRNAs with this method...
  86. pmc Expanding role of the jumonji C domain as an RNA hydroxylase
    Akiko Noma
    Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 285:34503-7. 2010
    ..The finding provides an insight into the expanding role of JmjC protein as an RNA hydroxylase...