Mikiko C Siomi


Affiliation: University of Tokyo
Country: Japan


  1. Nishida K, Iwasaki Y, Murota Y, Nagao A, Mannen T, Kato Y, et al. Respective functions of two distinct Siwi complexes assembled during PIWI-interacting RNA biogenesis in Bombyx germ cells. Cell Rep. 2015;10:193-203 pubmed publisher
  2. Yashiro R, Murota Y, Nishida K, Yamashiro H, Fujii K, Ogai A, et al. Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells. Cell Rep. 2018;23:3647-3657 pubmed publisher
    ..These results suggest that Piwi autoregulates its nuclear localization by exposing the NLS to Imp? upon piRNA loading. ..
  3. Sato K, Siomi M. Two distinct transcriptional controls triggered by nuclear Piwi-piRISCs in the Drosophila piRNA pathway. Curr Opin Struct Biol. 2018;53:69-76 pubmed publisher
    ..This review summarizes the mechanisms of PIWI-piRNA-mediated transcriptional controls in Drosophila ovaries. ..
  4. Hirakata S, Siomi M. piRNA biogenesis in the germline: From transcription of piRNA genomic sources to piRNA maturation. Biochim Biophys Acta. 2016;1859:82-92 pubmed publisher
    ..This article is part of a Special Issue entitled: Clues to long noncoding RNA taxonomy1, edited by Dr. Tetsuro Hirose and Dr. Shinichi Nakagawa. ..
  5. Sato K, Siomi M. Functional and structural insights into the piRNA factor Maelstrom. FEBS Lett. 2015;589:1688-93 pubmed publisher
    ..We summarize the current understanding of Mael functions in the piRNA pathway, particularly in Drosophila and in mice. ..
  6. Murota Y, Ishizu H, Nakagawa S, Iwasaki Y, Shibata S, Kamatani M, et al. Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly. Cell Rep. 2014;8:103-13 pubmed publisher
    ..We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells. ..
  7. Matsumoto N, Sato K, Nishimasu H, Namba Y, Miyakubi K, Dohmae N, et al. Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom. Cell Rep. 2015;11:366-75 pubmed publisher
    ..Our findings provide clues toward understanding the multiple roles of Mael in the piRNA pathway. ..
  8. Ishizu H, Iwasaki Y, Hirakata S, Ozaki H, Iwasaki W, Siomi H, et al. Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb. Cell Rep. 2015;12:429-40 pubmed publisher
    ..We thus propose that Yb determines primary piRNA sources through two modes of action: primary binding to cis elements to specify substrates and secondary binding to downstream regions to increase diversity in piRNA populations. ..
  9. Sato K, Iwasaki Y, Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, et al. Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline. Mol Cell. 2015;59:553-63 pubmed publisher
    ..Upon krimp-RNAi in OSCs, AGO3 loads with piRNAs, further showing the capacity of AGO3 for primary piRNA loading. We propose that Krimp enforces an antisense bias on piRNA pools by binding AGO3 and blocking its access to primary piRNAs. ..

More Information


  1. Matsumoto N, Nishimasu H, Sakakibara K, Nishida K, Hirano T, Ishitani R, et al. Crystal Structure of Silkworm PIWI-Clade Argonaute Siwi Bound to piRNA. Cell. 2016;167:484-497.e9 pubmed publisher
    ..Overall, this study provides a critical starting point toward a mechanistic understanding of piRNA-mediated transposon silencing. ..
  2. Ishizu H, Sumiyoshi T, Siomi M. Use of the CRISPR-Cas9 system for genome editing in cultured Drosophila ovarian somatic cells. Methods. 2017;126:186-192 pubmed publisher
    ..Here, we describe the detailed procedures for generating knockout and knockin OSC cells. ..