Affiliation: University of Tsukuba
Sasakura Y, Konno A, Mizuno K, Satoh N, Inaba K. Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos. Dev Dyn. 2008;237:39-50 pubmed
..This technique allowed us to isolate transgenic lines that express GFP in restricted tissues. This system provides an easy and efficient method for large-scale enhancer detection in Ciona intestinalis. ..
Sasakura Y. Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis. Dev Dyn. 2007;236:1758-67 pubmed
..This review specifically addresses recent achievements of transformation techniques in Ciona, as exemplified using the Minos system...
Sasakura Y, Yaguchi J, Yaguchi S, Yajima M. Excision and transposition activity of Tc1/mariner superfamily transposons in sea urchin embryos. Zoolog Sci. 2010;27:256-62 pubmed publisher
..This study suggests that Minos is active in a broad range of non-host organisms and can be used as a transformation tool in sea urchin embryos...
Sasakura Y, Kanda M, Ikeda T, Horie T, Kawai N, Ogura Y, et al
. Retinoic acid-driven Hox1 is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis. Development. 2012;139:2156-60 pubmed publisher
..Our results suggest that RA-Hox1 in the epidermal ectoderm played a key role in the acquisition of the otic placode during chordate evolution...