T Sakurai

Summary

Affiliation: University of Tsukuba
Country: Japan

Publications

  1. pmc Identification of a population of sleep-active cerebral cortex neurons
    Dmitry Gerashchenko
    Biosciences Division, SRI International, Menlo Park, CA 94025, USA
    Proc Natl Acad Sci U S A 105:10227-32. 2008
  2. ncbi request reprint Distribution of neuropeptide W immunoreactivity and mRNA in adult rat brain
    Yoji Kitamura
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Brain Res 1093:123-34. 2006
  3. doi request reprint GFP labeling of neurosecretory cells with the GAL4/UAS system in the silkmoth brain enables selective intracellular staining of neurons
    Tomoko Yamagata
    Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan
    Zoolog Sci 25:509-16. 2008
  4. ncbi request reprint [Discovery of orexin]
    Takeshi Sakurai
    Nihon Yakurigaku Zasshi 130:19-22. 2007
  5. ncbi request reprint Orexins and orexin receptors: implication in feeding behavior
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Regul Pept 85:25-30. 1999
  6. ncbi request reprint Structure and function of human prepro-orexin gene
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305 8575, Japan
    J Biol Chem 274:17771-6. 1999
  7. ncbi request reprint The neural circuit of orexin (hypocretin): maintaining sleep and wakefulness
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Ibaraki 305 8575, Japan
    Nat Rev Neurosci 8:171-81. 2007
  8. ncbi request reprint Roles of orexins and orexin receptors in central regulation of feeding behavior and energy homeostasis
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    CNS Neurol Disord Drug Targets 5:313-25. 2006
  9. ncbi request reprint [Regulatory mechanism of sleep/wakefulness states by orexin]
    Takeshi Sakurai
    Tanpakushitsu Kakusan Koso 52:1840-8. 2007
  10. ncbi request reprint Roles of orexin/hypocretin in regulation of sleep/wakefulness and energy homeostasis
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305 8575, Japan
    Sleep Med Rev 9:231-41. 2005

Collaborators

Detail Information

Publications77

  1. pmc Identification of a population of sleep-active cerebral cortex neurons
    Dmitry Gerashchenko
    Biosciences Division, SRI International, Menlo Park, CA 94025, USA
    Proc Natl Acad Sci U S A 105:10227-32. 2008
    ....
  2. ncbi request reprint Distribution of neuropeptide W immunoreactivity and mRNA in adult rat brain
    Yoji Kitamura
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Brain Res 1093:123-34. 2006
    ..These results suggest that NPW might be involved in the regulation of stress and emotive responses, especially in fear and anxiety-related physiological and behavioral functions...
  3. doi request reprint GFP labeling of neurosecretory cells with the GAL4/UAS system in the silkmoth brain enables selective intracellular staining of neurons
    Tomoko Yamagata
    Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan
    Zoolog Sci 25:509-16. 2008
    ..This work provides a novel method to visualize specific subsets of neurons in the silkmoth brain and to observe detailed structures in a single identified neuron from different individuals...
  4. ncbi request reprint [Discovery of orexin]
    Takeshi Sakurai
    Nihon Yakurigaku Zasshi 130:19-22. 2007
  5. ncbi request reprint Orexins and orexin receptors: implication in feeding behavior
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Regul Pept 85:25-30. 1999
    ..Actually, orexins have been reported to have roles in regulating drinking behavior, neuroendocrine function and the sleep-wake cycle...
  6. ncbi request reprint Structure and function of human prepro-orexin gene
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305 8575, Japan
    J Biol Chem 274:17771-6. 1999
    ..These mice might also be useful for examining the molecular mechanisms by which orexin gene expression is regulated...
  7. ncbi request reprint The neural circuit of orexin (hypocretin): maintaining sleep and wakefulness
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Ibaraki 305 8575, Japan
    Nat Rev Neurosci 8:171-81. 2007
    ..The orexin (hypocretin) system regulates sleep and wakefulness through interactions with systems that regulate emotion, reward and energy homeostasis...
  8. ncbi request reprint Roles of orexins and orexin receptors in central regulation of feeding behavior and energy homeostasis
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    CNS Neurol Disord Drug Targets 5:313-25. 2006
    ..Recent studies on afferent (input) systems of orexin neurons further suggest roles of orexin and orexin receptors in the coordination of feeding, arousal and emotion...
  9. ncbi request reprint [Regulatory mechanism of sleep/wakefulness states by orexin]
    Takeshi Sakurai
    Tanpakushitsu Kakusan Koso 52:1840-8. 2007
  10. ncbi request reprint Roles of orexin/hypocretin in regulation of sleep/wakefulness and energy homeostasis
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305 8575, Japan
    Sleep Med Rev 9:231-41. 2005
    ....
  11. ncbi request reprint [Roles of biologically active peptide in regulation of feeding behavior and energy homeostasis]
    Takeshi Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Nihon Yakurigaku Zasshi 122:236-42. 2003
    ..Many of these systems are regulated by peripheral metabolic cues including plasma leptin levels. This review summarizes roles of neuropeptides in the regulatory mechanism of feeding and energy homeostasis...
  12. ncbi request reprint Reverse pharmacology of orexin: from an orphan GPCR to integrative physiology
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Regul Pept 126:3-10. 2005
    ....
  13. ncbi request reprint Input of orexin/hypocretin neurons revealed by a genetically encoded tracer in mice
    Takeshi Sakurai
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305 8575, Japan
    Neuron 46:297-308. 2005
    ..These neuroanatomical findings provide important insights into the neural pathways that regulate sleep/wakefulness states...
  14. ncbi request reprint Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Nature 348:732-5. 1990
    ..These results indicate that this cDNA encodes a 'nonselective' subtype of the receptor which is different from the vascular smooth muscle receptor...
  15. ncbi request reprint Genetic ablation of orexin neurons in mice results in narcolepsy, hypophagia, and obesity
    J Hara
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Neuron 30:345-54. 2001
    ..Orexin/ataxin-3 mice provide a valuable model for studying the pathophysiology and treatment of narcolepsy...
  16. ncbi request reprint Hypothalamic orexin neurons regulate arousal according to energy balance in mice
    Akihiro Yamanaka
    Institute of Basic Medical Science, University of Tsukuba, 305 8575, Ibaraki, Japan
    Neuron 38:701-13. 2003
    ..Transgenic mice, in which orexin neurons are ablated, fail to respond to fasting with increased wakefulness and activity. These findings indicate that orexin neurons provide a crucial link between energy balance and arousal...
  17. ncbi request reprint Orexin-mediated feeding behavior involves both leptin-sensitive and -insensitive pathways
    Y Zhu
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Physiol Behav 77:251-7. 2002
    ..These observations suggest that NPY- and galanin-induced increases of feeding involve a leptin-sensitive pathway, while orexin-induced feeding involves both leptin-sensitive and -insensitive pathways...
  18. ncbi request reprint Differential expression of bovine adseverin in adrenal gland revealed by in situ hybridization. Cloning of a cDNA for adseverin
    S Nakamura
    Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan
    J Biol Chem 269:5890-6. 1994
    ..The observation strongly suggested that adseverin is not involved in secretory processes in general but is specifically involved in exocytosis...
  19. ncbi request reprint The Ca2(+)-dependent actin filament-severing activity of 74-kDa protein (adseverin) resides in its NH2-terminal half
    T Sakurai
    Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan
    J Biol Chem 266:4581-5. 1991
    ..The sequence homology and similar functional domain structure suggest a common structural basis for the calcium- and phospholipid-regulated actin-severing properties shared by adseverin, gelsolin, and villin...
  20. ncbi request reprint cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA
    T Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Japan
    Biochem Biophys Res Commun 175:44-7. 1991
    ..These findings suggest that ET-1 may play roles as a local mediator in multiple organs both within and outside the cardiovascular system in the rat...
  21. ncbi request reprint Molecular cloning of low-Ca(2+)-sensitive-type non-muscle alpha-actinin
    M Imamura
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Eur J Biochem 223:395-401. 1994
    ..The present results clearly demonstrate the existence of two structurally and functionally different types of non-muscle alpha-actinin; high-Ca(2+)-sensitive-type (NM1) and low-Ca(2+)-sensitive-type (NM2) alpha-actinin...
  22. ncbi request reprint Serotonergic regulation of the orexin/hypocretin neurons through the 5-HT1A receptor
    Yo Muraki
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    J Neurosci 24:7159-66. 2004
    ....
  23. ncbi request reprint Distribution of orexin neurons in the adult rat brain
    T Nambu
    Institute of Basic Medical Sciences, University of Tsukuba, 1 1, Tennohdai, Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Brain Res 827:243-60. 1999
    ....
  24. ncbi request reprint Orexin: a link between energy homeostasis and adaptive behaviour
    Takeshi Sakurai
    Department of Pharmacology, University of Tsukuba, Japan
    Curr Opin Clin Nutr Metab Care 6:353-60. 2003
    ..The purpose of this review is to summarize recent relevant findings on orexins, and discuss the physiological roles of these peptides...
  25. ncbi request reprint Cholecystokinin activates orexin/hypocretin neurons through the cholecystokinin A receptor
    Natsuko Tsujino
    Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    J Neurosci 25:7459-69. 2005
    ..The evidence presented here expands our understanding of the regulation of orexin neurons and the physiological role of CCK in the CNS...
  26. ncbi request reprint Cloning and functional expression of human cDNA for the ETB endothelin receptor
    A Sakamoto
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    Biochem Biophys Res Commun 178:656-63. 1991
    ..The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo...
  27. ncbi request reprint Difference in obesity phenotype between orexin-knockout mice and orexin neuron-deficient mice with same genetic background and environmental conditions
    Junko Hara
    Institute of Basic Medical Sciences, Department of Pharmacology, University of Tsukuba, Ibaraki 305 8575, Japan
    Neurosci Lett 380:239-42. 2005
    ..Our observation also suggests that the obesity observed in orexin neuron-deficient narcolepsy is dependent on the genetic background and environmental factors...
  28. doi request reprint Vasopressin increases locomotion through a V1a receptor in orexin/hypocretin neurons: implications for water homeostasis
    Tomomi Tsunematsu
    Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    J Neurosci 28:228-38. 2008
    ..This system appears to play a key role in water deprivation-induced hyperlocomotor activity, a response to dehydration that increases the chance of locating water in nature...
  29. ncbi request reprint Regulation of orexin neurons by the monoaminergic and cholinergic systems
    Akihiro Yamanaka
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Biochem Biophys Res Commun 303:120-9. 2003
    ..Our data suggest that orexin neurons have reciprocal neural circuitries between these nuclei for either a positive or negative feedback loop and orchestrate the activity of these neurons to regulate the vigilance states...
  30. ncbi request reprint Molecular identification of guanine-nucleotide-binding regulatory proteins which couple to endothelin receptors
    M Takigawa
    Department of Pharmacology, University of Tsukuba, Ibaraki, Japan
    Eur J Biochem 228:102-8. 1995
    ..The overall effects induced by endothelin may differ in cell types depending on the level of expression of each G-protein subtype in the cell...
  31. ncbi request reprint Orexin neuronal circuitry: role in the regulation of sleep and wakefulness
    Kousaku Ohno
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Front Neuroendocrinol 29:70-87. 2008
    ..This review discusses the mechanism by which orexins maintain sleep/wakefulness states, and how this mechanism relates to other systems that regulate emotion, reward, and energy homeostasis...
  32. ncbi request reprint Roles of orexins in regulation of feeding and wakefulness
    Takeshi Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Neuroreport 13:987-95. 2002
    ..Orexin neurons may provide an integrative link between peripheral metabolism and central regulation of behaviors required for an adaptive response to homeostatic challenges...
  33. ncbi request reprint Orexins activate histaminergic neurons via the orexin 2 receptor
    Akihiro Yamanaka
    Department of Pharmacology, Institute of Basic Medical Sciences, Tsukuba, Ibaraki, 305 8575, Japan
    Biochem Biophys Res Commun 290:1237-45. 2002
    ..Central application of pyrilamine significantly inhibited this response. These results strongly suggest that activation of histaminergic neurons by orexins might be important for modulation of the arousal...
  34. doi request reprint Orexins and orexin receptors: from molecules to integrative physiology
    Taizo Matsuki
    Department of Pharmacology, Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki, Japan
    Results Probl Cell Differ 46:27-55. 2008
    ..In this chapter, we discuss the current understanding of the physiological roles of orexins in regulation of arousal, sleep/wake states, energy homeostasis, and reward systems...
  35. ncbi request reprint Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice
    Masashi Akiyama
    Department of Pharmacology, School of Science and Engineering, Waseda University, 2 7 5 Higashifushimi, Nishitokyo, 202 0021, Japan
    Eur J Neurosci 20:3054-62. 2004
    ..Based on these results, we suggest that activity of the orexin neuron in the LHA contributes to the promotion and maintenance of FAA...
  36. ncbi request reprint Complete primary structure and tissue expression of chicken pectoralis M-protein
    J Noguchi
    Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan
    J Biol Chem 267:20302-10. 1992
    ..This indicates that M-protein, along with other thick filament-associated proteins such as C-protein, twichin, and titin, belongs to the superfamily of cytoskeletal proteins with immunoglobulin/fibronectin repeats...
  37. doi request reprint Effect of neuropeptide B on feeding behavior is influenced by endogenous corticotropin-releasing factor activities
    Shizu Aikawa
    Department of Cardiovascular Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305 8575, Japan
    Regul Pept 151:147-52. 2008
    ..In striking contrast, co-administration of astressin and NPB increased food intake under surgical stress conditions. These data suggest that the effects of NPB are greatly influenced by the endogenous CRFergic tone...
  38. ncbi request reprint The human prepro-orexin gene regulatory region that activates gene expression in the lateral region and represses it in the medial regions of the hypothalamus
    Takashi Moriguchi
    Departments of Pharmacology, Anatomy and Embryology, and Molecular and Developmental Biology, Institute of Basic Medical Sciences, and the Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8575, Japan
    J Biol Chem 277:16985-92. 2002
    ..Thus, OE1 contains crucial cis-acting elements regulating prepro-orexin gene expression specifically in the LHA...
  39. ncbi request reprint Effects of orexin on cultured porcine adrenal medullary and cortex cells
    Toru Nanmoku
    Department of Clinical Pathology, Institute of Clinical Medicine, University of Tsukuba, 1 1 1 Tenoudai, lbaraki 305 8575, Tsukuba, Japan
    Regul Pept 104:125-30. 2002
    ..These results indicate that orexin-A induces the release of catecholamine from porcine adrenal medullary cells, and aldosterone and cortisol from the cortex cells and has opposite effects on cAMP levels in adrenal medulla and cortex...
  40. ncbi request reprint Orexin neurons are directly and indirectly regulated by catecholamines in a complex manner
    Akihiro Yamanaka
    Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305 8575, Japan
    J Neurophysiol 96:284-98. 2006
    ..The evidence presented here revealed that orexin neurons are regulated by catecholamines in a complex manner...
  41. ncbi request reprint Cholinergic regulation of orexin/hypocretin neurons through M(3) muscarinic receptor in mice
    Kousaku Ohno
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Japan
    J Pharmacol Sci 106:485-91. 2008
    ..These results indicate that CCh activates 20% of orexin neurons through the M(3) muscarinic receptor and subsequent activation of nonselective cation channels...
  42. ncbi request reprint [Orexin]
    Takeshi Sakurai
    Institute of Basic Medical Sciences, University of Tsukuba
    Nihon Rinsho 63:600-2. 2005
  43. ncbi request reprint Orexin receptor type-1 couples exclusively to pertussis toxin-insensitive G-proteins, while orexin receptor type-2 couples to both pertussis toxin-sensitive and -insensitive G-proteins
    Yun Zhu
    Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan
    J Pharmacol Sci 92:259-66. 2003
    ..This suggests that although OX2R couples to multiple G-proteins, activation of neurons by orexins through OX2R is mediated via a PTX-insensitive, PLC dependent pathway...
  44. ncbi request reprint Cloning and expression of rat preproendothelin-3 cDNA
    R Shiba
    Department of Pharmacology, University of Tsukuba, Ibaraki, Japan
    Biochem Biophys Res Commun 186:588-94. 1992
    ..A 2.0-kb and a 3.3-kb mRNA were also detected in the eye ball and small intestine, respectively. The distinct distribution of rat preproET-3 mRNA from that of preproET-1 mRNA suggested that ET-1 and ET-3 played different roles...
  45. ncbi request reprint Comparison between the gelsolin and adseverin domain structure
    T Sakurai
    Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan
    J Biol Chem 266:15979-83. 1991
    ..Based on these observations, we propose that adseverin has a functional domain structure similar to that of the gelsolin and villin core...
  46. ncbi request reprint Involvement of the serotonergic system in orexin-induced behavioral alterations in rats
    Ichiyo Matsuzaki
    Department of Environmental Health, Institute of Community Medicine, University of Tsukuba, Ibaraki 305 8575, Tsukuba, Japan
    Regul Pept 104:119-23. 2002
    ..These results suggest that the dopaminergic system might be involved in orexin-induced hyperlocomotion, while both the serotonergic system as well as the dopaminergic system might be involved in orexin-induced behavioral responses...
  47. ncbi request reprint Orexins (hypocretins) directly interact with neuropeptide Y, POMC and glucose-responsive neurons to regulate Ca 2+ signaling in a reciprocal manner to leptin: orexigenic neuronal pathways in the mediobasal hypothalamus
    Shinji Muroya
    Department of Physiology, Division of Integrative Physiology, Jichi Medical School, Minamikawachi, Kawachi, Tochigi 329 0498, Japan
    Eur J Neurosci 19:1524-34. 2004
    ..Orexin-A evokes Ca(2+) signaling in NPY neurons via OX(1)R-PLC-PKC and IP(3) pathways. These neural pathways and intracellular signaling mechanisms may play key roles in the orexigenic action of orexins...
  48. ncbi request reprint Hypocretin/Orexin excites hypocretin neurons via a local glutamate neuron-A potential mechanism for orchestrating the hypothalamic arousal system
    Ying Li
    Department of Neurosurgery, Yale University, New Haven, CT 06520, USA
    Neuron 36:1169-81. 2002
    ..Norepinephrine and serotonin, transmitters of other arousal systems, decreased spike frequency and evoked outward currents, whereas acetylcholine and histamine had little effect...
  49. ncbi request reprint Activation of orexin neurones after noxious but not conditioned fear stimuli in rats
    Lingling Zhu
    Department of Physiology, Jichi Medical School, Minamikawachi Machi, Tochigi 329 0498, Japan
    Neuroreport 13:1351-3. 2002
    ..This finding is consistent with an idea that orexin neurones are involved in stress-induced food intake...
  50. ncbi request reprint Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor
    Koji Toshinai
    Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, Japan
    Endocrinology 147:2306-14. 2006
    ..Central des-acyl ghrelin may activate orexin-expressing neurons, perhaps functioning in feeding regulation through interactions with a target protein distinct from the GHS-R...
  51. pmc Hypocretin/orexin and nociceptin/orphanin FQ coordinately regulate analgesia in a mouse model of stress-induced analgesia
    Xinmin Xie
    Biosciences Division, SRI International, Menlo Park, California 94025, USA
    J Clin Invest 118:2471-81. 2008
    ..These results establish what is, to our knowledge, a novel interaction between the N/OFQ and Hcrt systems in which the corticotropin-releasing factor and N/OFQ systems coordinately modulate the Hcrt neurons to regulate SIA...
  52. pmc Sleep/wake fragmentation disrupts metabolism in a mouse model of narcolepsy
    Shengwen Zhang
    Psychiatry and Behavioural Sciences, Stanford University, Palo Alto, CA 94304 5742, USA
    J Physiol 581:649-63. 2007
    ..This investigation illustrates the need for coordinated study of multiple phenotypes in mouse models with altered sleep/wake patterns...
  53. ncbi request reprint Effects of anesthetics on the function of orexin-1 receptors expressed in Xenopus oocytes
    Kouichiro Minami
    Department of Anesthesiology, Jichi Medical University, Tochigi, Japan
    Pharmacology 79:236-42. 2007
    ..The inhibitory effects of all of the compounds tested were shown to be caused by the inhibition of OX1R function. These results may, at least in part, explain their hypnotic effects...
  54. ncbi request reprint Dexmedetomidine inhibits muscarinic type 3 receptors expressed in Xenopus oocytes and muscarine-induced intracellular Ca2+ elevation in cultured rat dorsal root ganglia cells
    Atsushi Takizuka
    Department of Anesthesiology, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishiku, Kitakyushu, 807 8555, Japan
    Naunyn Schmiedebergs Arch Pharmacol 375:293-301. 2007
    ..Dexmedetomidine inhibits the function of M(3) receptors, in addition to its agonistic effects on alpha(2)-adrenoceptors, which provides further insight into the pharmacological properties of dexmedetomidine...
  55. pmc Entrainment of temperature and activity rhythms to restricted feeding in orexin knock out mice
    Satvinder Kaur
    West Roxbury VA Medical Center and Harvard Medical School, 1400 VFW Parkway, West Roxbury, MA 02132, USA
    Brain Res 1205:47-54. 2008
    ..We conclude that orexin is not required for entrainment of activity and temperature to a restricted feeding schedule, but is required for the robust expression of gross locomotor activity in anticipation of the scheduled feeding...
  56. doi request reprint The NPB/NPW neuropeptide system and its role in regulating energy homeostasis, pain, and emotion
    Mari Hondo
    Department of Molecular Neuroscience and Integrative Physiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan
    Results Probl Cell Differ 46:239-56. 2008
    ..Future studies of NPB/NPW using both pharmacological and phenotypic analysis of genetically engineered mice will lead to further elucidation of the physiological role of this novel neuropeptide system...
  57. pmc A neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice
    Shinobu Takayasu
    Yanagisawa Orphan Receptor Project, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Tokyo 135 0064, Japan
    Proc Natl Acad Sci U S A 103:7438-43. 2006
    ..Our findings suggest that QRFP and GPR103A/B may regulate diverse neuroendocrine and behavioral functions and implicate this neuropeptide system in metabolic syndrome...
  58. pmc GABA(B) receptor-mediated modulation of hypocretin/orexin neurones in mouse hypothalamus
    Xinmin Xie
    Biosciences Division, SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025, USA
    J Physiol 574:399-414. 2006
    ....
  59. pmc Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8
    Hirokazu Tanaka
    Howard Hughes Medical Institute and Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75390 9050, USA
    Proc Natl Acad Sci U S A 100:6251-6. 2003
    ..These findings suggest diverse physiological functions of NPB and NPW in the central nervous system, acting as endogenous ligands on GPR7 andor GPR8...
  60. pmc Orexin peptides prevent cataplexy and improve wakefulness in an orexin neuron-ablated model of narcolepsy in mice
    Michihiro Mieda
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 101:4649-54. 2004
    ..Orexin receptor agonists would be of potential value for treating human narcolepsy...
  61. ncbi request reprint Ghrelin-induced food intake is mediated via the orexin pathway
    Koji Toshinai
    Department of Internal Medicine, Miyazaki Medical College, Miyazaki 889 1692, Japan
    Endocrinology 144:1506-12. 2003
    ..Ghrelin-induced feeding was also suppressed in orexin knockout mice. This study identifies a novel hypothalamic pathway that links ghrelin and orexin in the regulation of feeding behavior and energy homeostasis...
  62. ncbi request reprint Expression of a poly-glutamine-ataxin-3 transgene in orexin neurons induces narcolepsy-cataplexy in the rat
    Carsten T Beuckmann
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9050, USA
    J Neurosci 24:4469-77. 2004
    ..These findings indicate that the orexin/ataxin-3 transgenic rat could provide a useful model of human narcolepsy...
  63. ncbi request reprint Interaction between the corticotropin-releasing factor system and hypocretins (orexins): a novel circuit mediating stress response
    Raphaelle Winsky-Sommerer
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 24:11439-48. 2004
    ..We propose that, after stressor stimuli, CRF stimulates the release of hypocretins and that this circuit contributes to activation and maintenance of arousal associated with the stress response...
  64. ncbi request reprint Behavioral state instability in orexin knock-out mice
    Takatoshi Mochizuki
    Department of Neurology, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    J Neurosci 24:6291-300. 2004
    ..Instead, the fragmented behavior of orexin KO mice may be best described as behavioral state instability, with apparently low thresholds to transition between states...
  65. ncbi request reprint Orexin neurons function in an efferent pathway of a food-entrainable circadian oscillator in eliciting food-anticipatory activity and wakefulness
    Michihiro Mieda
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9050, USA
    J Neurosci 24:10493-501. 2004
    ..Orexin neurons thus convey an efferent signal from putative food-entrainable oscillator or oscillators to increase wakefulness and locomotor activity...
  66. ncbi request reprint Variants of the orexin2/hcrt2 receptor gene identified in patients with excessive daytime sleepiness and patients with Tourette's syndrome comorbidity
    Miles D Thompson
    Department of Pharmacology, University of Toronto, Toronto, Ontario, Canada
    Am J Med Genet B Neuropsychiatr Genet 129:69-75. 2004
    ..Further work is warranted to study the variability of the orexin/hypocretin system in a variety of disorders characterized by EDS...
  67. ncbi request reprint Association of an orexin 1 receptor 408Val variant with polydipsia-hyponatremia in schizophrenic subjects
    Joanne Meerabux
    Laboratory for Molecular Psychiatry, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Biol Psychiatry 58:401-7. 2005
    ..Both angiotensin-converting enzyme (ACE) and orexin (hypocretin) signaling can modulate drinking behavior through interactions with the dopaminergic system...
  68. ncbi request reprint Orexin neuron-mediated skeletal muscle vasodilation and shift of baroreflex during defense response in mice
    Wei Zhang
    Department of Molecular and Integrative Physiology, Chiba University Graduate School of Medicine, 1 8 1 Chuo ku, Chiba 260 8670, Japan
    Am J Physiol Regul Integr Comp Physiol 290:R1654-63. 2006
    ....
  69. ncbi request reprint NrCAM in addiction vulnerability: positional cloning, drug-regulation, haplotype-specific expression, and altered drug reward in knockout mice
    Hiroki Ishiguro
    Molecular Neurobiology Branch, NIDA IRP, NIH, Baltimore, MD 21224, USA
    Neuropsychopharmacology 31:572-84. 2006
    ..These observations support NrCAM as a positionally cloned and drug-regulated gene whose variants are likely to change expression and alter substance abuse vulnerabilities in human addictions and animal models of drug reward...
  70. pmc Sex difference in body weight gain and leptin signaling in hypocretin/orexin deficient mouse models
    Nobuhiro Fujiki
    Center for Narcolepsy, Stanford Sleep Research Center, 701B Welch Rd, Palo Alto, CA 94304, United States
    Peptides 27:2326-31. 2006
    ..However, multiple factors are also likely to affect this phenotype, and a sex difference specific alteration of leptin-hypocretin signaling may be involved...
  71. pmc Elevated body temperature during sleep in orexin knockout mice
    Takatoshi Mochizuki
    Department of Neurology, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA
    Am J Physiol Regul Integr Comp Physiol 291:R533-40. 2006
    ..In addition, because heat loss is an essential aspect of sleep, the blunted fall in Tb of orexin KO mice may provide an explanation for the fragmented sleep of narcolepsy...
  72. ncbi request reprint Direct involvement of orexinergic systems in the activation of the mesolimbic dopamine pathway and related behaviors induced by morphine
    Minoru Narita
    Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo 142 8501, Japan
    J Neurosci 26:398-405. 2006
    ..These findings provide new evidence that orexin-containing neurons in the VTA are directly implicated in the rewarding effect and hyperlocomotion induced by morphine through activation of the mesolimbic dopamine pathway in rodents...
  73. ncbi request reprint Reduced expression of the endothelin receptor type B gene in piebald mice caused by insertion of a retroposon-like element in intron 1
    Takahisa Yamada
    Howard Hughes Medical Institute and the Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9050, USA
    J Biol Chem 281:10799-807. 2006
    ....
  74. ncbi request reprint Insect sex-pheromone signals mediated by specific combinations of olfactory receptors
    Takao Nakagawa
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277 8562, Japan
    Science 307:1638-42. 2005
    ..The same effects were also observed for general ORs. Both odorant and pheromone signaling pathways are mediated by means of a common mechanism in insects...
  75. ncbi request reprint Rudiment incisors survive and erupt as supernumerary teeth as a result of USAG-1 abrogation
    Akiko Murashima-Suginami
    Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 359:549-55. 2007
    ..USAG-1 abrogation rescued apoptotic elimination of odontogenic mesenchymal cells. Based upon these results, we conclude that USAG-1 controls the number of teeth in the maxillary incisor region by regulating apoptosis...
  76. ncbi request reprint [Molecular mechanisms underlying sex-pheromone reception in insects]
    Takao Nakagawa
    Tanpakushitsu Kakusan Koso 50:1563-70. 2005
  77. ncbi request reprint A role for Nr-CAM in the patterning of binocular visual pathways
    Scott E Williams
    Center for Neurobiology and Behavior and Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Neuron 50:535-47. 2006
    ..Finally, we demonstrate that EphB1/ephrin-B2-mediated repulsion and Nr-CAM-mediated attraction comprise distinct molecular programs that each contributes to the proper formation of binocular visual pathways...