Mitsuru Okuwaki

Summary

Affiliation: University of Tsukuba
Country: Japan

Publications

  1. pmc Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association
    Miharu Hisaoka
    Faculty of Medicine, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8575, Japan
    Nucleic Acids Res 42:1180-95. 2014
  2. pmc Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling
    Mitsuru Okuwaki
    Faculty of Medicine and Graduate School of Comprehensive Human Sciences, Initiative for Promotion of Young Scientists Independent Research, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8577, Japan
    Nucleic Acids Res 40:4861-78. 2012
  3. ncbi request reprint The structure and functions of NPM1/Nucleophsmin/B23, a multifunctional nucleolar acidic protein
    Mitsuru Okuwaki
    Graduate School of Comprehensive Human Sciences and Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Japan
    J Biochem 143:441-8. 2008
  4. ncbi request reprint [Structure and function of the nucleolus]
    Mitsuru Okuwaki
    Tanpakushitsu Kakusan Koso 51:1950-6. 2006
  5. doi request reprint Functional characterization of human nucleosome assembly protein 1-like proteins as histone chaperones
    Mitsuru Okuwaki
    Initiative for the Promotion of Young Scientists Independent Research, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8577, Japan
    Genes Cells 15:13-27. 2010
  6. pmc Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I
    Mitsuru Okuwaki
    Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8575, Japan
    Mol Cell Biol 25:10639-51. 2005
  7. pmc Regulation of nucleolar chromatin by B23/nucleophosmin jointly depends upon its RNA binding activity and transcription factor UBF
    Miharu Hisaoka
    Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Japan
    Mol Cell Biol 30:4952-64. 2010
  8. pmc Transcription regulation of the rRNA gene by a multifunctional nucleolar protein, B23/nucleophosmin, through its histone chaperone activity
    Kensaku Murano
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    Mol Cell Biol 28:3114-26. 2008
  9. doi request reprint Role of Template Activating Factor-I as a chaperone in linker histone dynamics
    Kohsuke Kato
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    J Cell Sci 124:3254-65. 2011
  10. ncbi request reprint Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene
    Shuhei Ueshima
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305, Japan
    Biochem Biophys Res Commun 443:22-7. 2014

Collaborators

  • Kyosuke Nagata
  • Fumitaka Momose
  • Bela Gyurcsik
  • Yoshifumi Nishimura
  • H Hashimoto
  • Junn Yanagisawa
  • Miharu Hisaoka
  • Kensaku Murano
  • Shuhei Ueshima
  • Mohammad Abdus Samad
  • Kohsuke Kato
  • Hirohito Haruki
  • Akiko Murayama
  • Abarna Thiru
  • Hans van Leeuwen
  • Robert F Newbold
  • Michiko Kumakura
  • Tetsuro Komatsu
  • Akiyoshi Fukamizu
  • Hiroshi Minami
  • Hiroaki Daitoku
  • Takao Kuroda
  • Kayoko Yasuzawa-Tanaka
  • Akiko Fujimura
  • Keiji Kimura
  • Toshiyuki Shimizu
  • Shohei Oie
  • Kazuji Ohmori
  • Mary Miyaji-Yamaguchi
  • Makoto Miyagishi
  • Kazunari Taira
  • Alain Verreault
  • Ernest D Laue
  • Natalia V Murzina
  • Daniel Nietlispach
  • Debbie Lyon
  • Peter R Nielsen
  • Helen R Mott
  • Miriam Hirshberg
  • Rui Hong
  • Debabrata Chakravarti
  • Peter O'Hare

Detail Information

Publications21

  1. pmc Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association
    Miharu Hisaoka
    Faculty of Medicine, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8575, Japan
    Nucleic Acids Res 42:1180-95. 2014
    ....
  2. pmc Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling
    Mitsuru Okuwaki
    Faculty of Medicine and Graduate School of Comprehensive Human Sciences, Initiative for Promotion of Young Scientists Independent Research, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8577, Japan
    Nucleic Acids Res 40:4861-78. 2012
    ..Our results proposed a novel mechanism whereby three NPM proteins cooperatively regulate chromatin disassembly and assembly in the early embryo and in somatic cells...
  3. ncbi request reprint The structure and functions of NPM1/Nucleophsmin/B23, a multifunctional nucleolar acidic protein
    Mitsuru Okuwaki
    Graduate School of Comprehensive Human Sciences and Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Japan
    J Biochem 143:441-8. 2008
    ..In this review, the basic structure and functions of B23 as well as the regulation of these functions are summarized...
  4. ncbi request reprint [Structure and function of the nucleolus]
    Mitsuru Okuwaki
    Tanpakushitsu Kakusan Koso 51:1950-6. 2006
  5. doi request reprint Functional characterization of human nucleosome assembly protein 1-like proteins as histone chaperones
    Mitsuru Okuwaki
    Initiative for the Promotion of Young Scientists Independent Research, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8577, Japan
    Genes Cells 15:13-27. 2010
    ..These results suggest that human NAP1-like proteins play overlapping roles in transport and deposition of histone H2A-H2B or H2A variants-H2B dimers on chromatin and nonoverlapping roles in nucleosome disassembly...
  6. pmc Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I
    Mitsuru Okuwaki
    Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8575, Japan
    Mol Cell Biol 25:10639-51. 2005
    ..Thus, both the acidic nature of NAP-I and its other functional structure(s) may be essential to mediate the assembly and disassembly of the dimers in NCPs...
  7. pmc Regulation of nucleolar chromatin by B23/nucleophosmin jointly depends upon its RNA binding activity and transcription factor UBF
    Miharu Hisaoka
    Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Japan
    Mol Cell Biol 30:4952-64. 2010
    ....
  8. pmc Transcription regulation of the rRNA gene by a multifunctional nucleolar protein, B23/nucleophosmin, through its histone chaperone activity
    Kensaku Murano
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    Mol Cell Biol 28:3114-26. 2008
    ..Expression of B23DeltaC decreased rRNA gene transcription and the rate of cell proliferation. These results suggest that B23 is involved in the transcription regulation of the rRNA gene as a nucleolar histone chaperone...
  9. doi request reprint Role of Template Activating Factor-I as a chaperone in linker histone dynamics
    Kohsuke Kato
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    J Cell Sci 124:3254-65. 2011
    ..Therefore, we propose that TAF-I is a key molecule that regulates linker histone-mediated chromatin assembly and disassembly...
  10. ncbi request reprint Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene
    Shuhei Ueshima
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305, Japan
    Biochem Biophys Res Commun 443:22-7. 2014
    ..However, we demonstrated that UBF alone was not sufficient to recruit these pre-rRNA processing factors to r-chromatin. Thus, UBF is necessary but not sufficient for the associations between pre-rRNA processing factors and r-chromatin...
  11. doi request reprint B23/nucleophosmin is involved in regulation of adenovirus chromatin structure at late infection stages, but not in virus replication and transcription
    Mohammad Abdus Samad
    Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8575, Japan
    J Gen Virol 93:1328-38. 2012
    ..In addition, these results suggest that proper viral chromatin assembly, regulated in part by B23, is crucial for the maturation of infectious virus particles...
  12. pmc Involvement of template-activating factor I/SET in transcription of adenovirus early genes as a positive-acting factor
    Hirohito Haruki
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    J Virol 80:794-801. 2006
    ..These results provide evidence that TAF-I plays an important role in the early stages of the adenovirus infection cycle...
  13. ncbi request reprint Physical and functional interaction between a nucleolar protein nucleophosmin/B23 and adenovirus basic core proteins
    Mohammad Abdus Samad
    Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba, Japan
    FEBS Lett 581:3283-8. 2007
    ..These results indicate that B23 functions as a chaperone in the viral chromatin assembly process in infected cells...
  14. pmc Histone acetylation-independent transcription stimulation by a histone chaperone
    Kohsuke Kato
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    Nucleic Acids Res 35:705-15. 2007
    ..These results suggest that TAF-I remodel the chromatin structure through its interaction with the core domain of the histones, including the histone fold, and this mechanism is independent of the histone acetylation status...
  15. pmc The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype
    Mitsuru Okuwaki
    Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba 305 8575, Japan
    Mol Biol Cell 13:2016-30. 2002
    ..1, those of which were incapable of binding to RNA, were dispersed throughout the nucleoplasm and cytoplasm, respectively. These results suggest that nucleolar localization of B23.1 is mediated by its ability to associate with RNA...
  16. doi request reprint Epigenetic control of rDNA loci in response to intracellular energy status
    Akiko Murayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8572, Japan
    Cell 133:627-39. 2008
    ..These findings provide key insight into the mechanisms of energy homeostasis in cells...
  17. ncbi request reprint Reconstitution of human rRNA gene transcription in mouse cells by a complete SL1 complex
    Kensaku Murano
    Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8575, Japan
    J Cell Sci 127:3309-19. 2014
    ..We conclude that four human TAFIs are necessary and sufficient to overcome the barrier of species specificity for human rDNA transcription in mouse cells. ..
  18. ncbi request reprint Ternary complex formation between DNA-adenovirus core protein VII and TAF-Ibeta/SET, an acidic molecular chaperone
    Hirohito Haruki
    Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    FEBS Lett 555:521-7. 2003
    ..In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA-protein VII complexes...
  19. ncbi request reprint Herpes simplex virus type 1 tegument protein VP22 interacts with TAF-I proteins and inhibits nucleosome assembly but not regulation of histone acetylation by INHAT
    Hans van Leeuwen
    Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    J Gen Virol 84:2501-10. 2003
    ..Consistent with this possibility was the observation that overexpression of TAF-I in transfected cells interferes with the progression of HSV-1 infection...
  20. pmc Structural basis of HP1/PXVXL motif peptide interactions and HP1 localisation to heterochromatin
    Abarna Thiru
    Department of Biochemistry, Cambridge Centre for Molecular Recognition, University of Cambridge, UK
    EMBO J 23:489-99. 2004
    ..This finding implies a further complexity to the histone code for regulation of chromatin structure and suggests how binding of HP1 family proteins may lead to its condensation...
  21. ncbi request reprint Maintenance DNA methylation of nucleosome core particles
    Mitsuru Okuwaki
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:2904-12. 2004
    ....