Tetsuo Nagano

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint [Fluorescence spectroscopy]
    Tetsuo Nagano
    Tanpakushitsu Kakusan Koso 49:1700-6. 2004
  2. doi request reprint Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region
    Takahiro Egawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Commun (Camb) 47:4162-4. 2011
  3. ncbi request reprint Practical methods for detection of nitric oxide
    T Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Luminescence 14:283-90. 1999
  4. ncbi request reprint Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Bioorg Med Chem Lett 18:3752-5. 2008
  5. pmc Bioimaging probes for reactive oxygen species and reactive nitrogen species
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences and Chemical Biology Research Initiative, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Clin Biochem Nutr 45:111-24. 2009
  6. ncbi request reprint [Development and biological applications of various bioimaging probes]
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
    Yakugaku Zasshi 126:901-13. 2006
  7. ncbi request reprint [Chemical biology: overview]
    Tetsuo Nagano
    Tanpakushitsu Kakusan Koso 52:1519-23. 2007
  8. pmc Development of fluorescent probes for bioimaging applications
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:837-47. 2010
  9. ncbi request reprint Bioimaging of nitric oxide
    Tetsuo Nagano
    Graduate School of Pharmacological Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Rev 102:1235-70. 2002
  10. pmc Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizer
    Takatoshi Yogo
    Graduate School of Pharmaceutical Sciences and Department of Pharmacology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 105:28-32. 2008

Detail Information

Publications119 found, 100 shown here

  1. ncbi request reprint [Fluorescence spectroscopy]
    Tetsuo Nagano
    Tanpakushitsu Kakusan Koso 49:1700-6. 2004
  2. doi request reprint Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region
    Takahiro Egawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Commun (Camb) 47:4162-4. 2011
    ..To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells...
  3. ncbi request reprint Practical methods for detection of nitric oxide
    T Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Luminescence 14:283-90. 1999
    ..In this review, we discuss practical methods for NO detection, which are useful for the detection of small amounts of NO from cNOS and for the bioimaging of living cells and cultured tissues...
  4. ncbi request reprint Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Bioorg Med Chem Lett 18:3752-5. 2008
    ..Because the probe contains a general kinase inhibitor, staurosporine, we believe that this homogeneous, high-throughput, and simple method can be applied to the inhibitor screening of other kinases as well...
  5. pmc Bioimaging probes for reactive oxygen species and reactive nitrogen species
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences and Chemical Biology Research Initiative, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Clin Biochem Nutr 45:111-24. 2009
    ....
  6. ncbi request reprint [Development and biological applications of various bioimaging probes]
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
    Yakugaku Zasshi 126:901-13. 2006
    ..More than twenty probes for imaging of nitric oxide, beta-galactosidase, highly reactive oxygen species, zinc ion et al. have been developed according to precise and rational design strategies based on PeT mechanism...
  7. ncbi request reprint [Chemical biology: overview]
    Tetsuo Nagano
    Tanpakushitsu Kakusan Koso 52:1519-23. 2007
  8. pmc Development of fluorescent probes for bioimaging applications
    Tetsuo Nagano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:837-47. 2010
    ..The review also describes some applications of the probes developed by our group to in vitro and in vivo systems...
  9. ncbi request reprint Bioimaging of nitric oxide
    Tetsuo Nagano
    Graduate School of Pharmacological Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Rev 102:1235-70. 2002
  10. pmc Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizer
    Takatoshi Yogo
    Graduate School of Pharmaceutical Sciences and Department of Pharmacology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 105:28-32. 2008
    ....
  11. doi request reprint Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization
    Masayo Sakabe
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 135:409-14. 2013
    ....
  12. ncbi request reprint Selective zinc sensor molecules with various affinities for Zn2+, revealing dynamics and regional distribution of synaptically released Zn2+ in hippocampal slices
    Kensuke Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:10197-204. 2005
    ..We believe that the use of various combinations of ZnAF family members will offer unprecedented versatility for fluorescence-microscopic imaging of Zn(2+) in biological applications...
  13. doi request reprint Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    ACS Chem Biol 6:600-8. 2011
    ..e., a series of pH-sensors for use in acidic environments and a Zn(2+) sensor. We synthesized these probes and confirmed that they work well...
  14. ncbi request reprint Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications
    Kenjiro Hanaoka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 126:12470-6. 2004
    ..This novel Zn(2+)-selective luminescent lanthanide chemosensor [Eu-7]should be an excellent lead compound for the development of a range of novel luminescent lanthanide chemosensors for biological applications...
  15. doi request reprint Fluorescence probe for lysophospholipase C/NPP6 activity and a potent NPP6 inhibitor
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Am Chem Soc 133:12021-30. 2011
    ..21 μM. Our NPP6-selective fluorescence probe, TG-mPC, and the inhibitor are expected to be useful to elucidate the biological function of NPP6...
  16. ncbi request reprint Modification of intracellular Ca2+ dynamics by laser inactivation of inositol 1,4,5-trisphosphate receptor using membrane-permeant probes
    Takatoshi Yogo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Biol 11:1053-8. 2004
    ..These results demonstrate the potency of the smCALI technique for the study of the roles of IP3R in complex intracellular Ca2+ dynamics...
  17. doi request reprint Calcineurin is critical for sodium-induced neointimal formation in normotensive and hypertensive rats
    Ryo Takeda
    Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    Am J Physiol Heart Circ Physiol 294:H2871-8. 2008
    ..These results also suggest that calcineurin plays a pivotal role in this process...
  18. doi request reprint TokyoGreen derivatives as specific and practical fluorescent probes for UDP-glucuronosyltransferase (UGT) 1A1
    Takuya Terai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo, Japan
    Chem Commun (Camb) 49:3101-3. 2013
    ..A rapid, specific, and sensitive assay of the enzyme was achieved simply by monitoring the change in fluorescence intensity. We also designed and developed the first "turn-on" fluorescent probes for UGTs...
  19. ncbi request reprint Rational principles for modulating fluorescence properties of fluorescein
    Tasuku Ueno
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 126:14079-85. 2004
    ..This information provides a basis for a practical strategy for rational design of novel functional fluorescence probes...
  20. pmc Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes
    Yasuteru Urano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan
    Nat Med 15:104-9. 2009
    ..The design concept can be widely adapted to cancer-specific, cell surface-targeting molecules that result in cellular internalization...
  21. ncbi request reprint A new constitutively active mutant of AMP-activated protein kinase inhibits anoxia-induced apoptosis of vascular endothelial cell
    Daisuke Nagata
    Department of Molecular Research for Vascular Diseases, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Hypertens Res 32:133-9. 2009
    ..In conclusion, NcaAMPK inhibited anoxia-induced apoptosis in vascular endothelial cells through Akt activation, suggesting that activation of AMPK might protect against ischemic vascular injury...
  22. doi request reprint Design and synthesis of highly sensitive fluorogenic substrates for glutathione S-transferase and application for activity imaging in living cells
    Yuuta Fujikawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 130:14533-43. 2008
    ..These results indicate that the newly developed fluorogenic substrates should be useful not only for high-throughput GST-inhibitor screening but also for studies on the mechanisms of drug resistance in cancer cells...
  23. doi request reprint Diced electrophoresis gel assay for screening enzymes with specified activities
    Toru Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 135:6002-5. 2013
    ..By developing fluorescent substrates that mimic the structure of neutrophil chemoattractants, we could identify enzymes involved in metabolic inactivation of the chemoattractants...
  24. doi request reprint Design strategy for a near-infrared fluorescence probe for matrix metalloproteinase utilizing highly cell permeable boron dipyrromethene
    Takuya Myochin
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Am Chem Soc 134:13730-7. 2012
    ..This simple design strategy should be applicable to develop a range of sensitive, rapidly responsive NIR fluorescence probes not only for MMP activity, but also for other proteases...
  25. doi request reprint Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold
    Daihi Oushiki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Anal Chem 84:4404-10. 2012
    ..Our strategy should be applicable to obtain NIR fluorescence probes for a wide range of target enzymes...
  26. doi request reprint A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Commun (Camb) 48:3091-3. 2012
    ..This probe is expected to be useful for monitoring the dynamics of ROS production continuously in vivo...
  27. doi request reprint Rational development of caged-biotin protein-labeling agents and some applications in live cells
    Takuya Terai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Biol 18:1261-72. 2011
    ..We also demonstrated the applicability of these compounds for UV-irradiated-cell-specific drug delivery by using caged-biotin-labeled cells, a prodrug, and streptavidin conjugated with a prodrug-activating enzyme...
  28. ncbi request reprint Design and synthesis of a library of BODIPY-based environmental polarity sensors utilizing photoinduced electron-transfer-controlled fluorescence ON/OFF switching
    Hisato Sunahara
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 129:5597-604. 2007
    ..The polarity at the surface of albumin was concluded to be similar to that of acetone, while the polarity of the internal membranes of HeLa cells was similar to that of dichloromethane...
  29. doi request reprint Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    J Am Chem Soc 131:6058-9. 2009
    ..Such activatable photosensitizers should allow more refined PDT without the side effect of prolonged light sensitivity and should also be useful as tools for reporter enzyme expression-specific cell ablation...
  30. doi request reprint Blockade of endogenous proinflammatory cytokines ameliorates endothelial dysfunction in obese Zucker rats
    Hiroaki Nishimatsu
    Department of Urology, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    Hypertens Res 31:737-43. 2008
    ....
  31. doi request reprint Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study
    Toru Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Chem Commun (Camb) 47:10055-7. 2011
    ..We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor...
  32. doi request reprint Development of a far-red to near-infrared fluorescence probe for calcium ion and its application to multicolor neuronal imaging
    Takahiro Egawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 133:14157-9. 2011
    ....
  33. ncbi request reprint A Gd3+-based magnetic resonance imaging contrast agent sensitive to beta-galactosidase activity utilizing a receptor-induced magnetization enhancement (RIME) phenomenon
    Kenjiro Hanaoka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 14:987-95. 2008
    ....
  34. doi request reprint Molecular design strategies for near-infrared ratiometric fluorescent probes based on the unique spectral properties of aminocyanines
    Kazuki Kiyose
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 15:9191-200. 2009
    ..These strategies were validated by obtaining tricarbocyanine-based ratiometric NIR fluorescent probes for esterase and for pH level...
  35. doi request reprint Screening and X-ray crystal structure-based optimization of autotaxin (ENPP2) inhibitors, using a newly developed fluorescence probe
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    ACS Chem Biol 8:1713-21. 2013
    ..These inhibitors are expected to be useful tools to understand the roles of ATX in vitro and in vivo and may also be candidate anti-ATX therapeutic agents. ..
  36. doi request reprint Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells
    Shodai Takahashi
    Faculty of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 134:19588-91. 2012
    ..Upon recovery of normoxia, the radical is immediately reoxidized to QSY-21, with loss of fluorescence due to restoration of FRET. We show that this probe, RHyCy5, can monitor repeated hypoxia-normoxia cycles in live cells...
  37. doi request reprint Highly activatable and environment-insensitive optical highlighters for selective spatiotemporal imaging of target proteins
    Tomonori Kobayashi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 134:11153-60. 2012
    ..We also demonstrate highlighting of cells of interest in live zebrafish embryos, using the fusion protein of histone 2A with SNAP tag...
  38. doi request reprint Red fluorescent scaffold for highly sensitive protease activity probes
    Yu Kushida
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Bioorg Med Chem Lett 22:3908-11. 2012
    ..Caspase-3-mediated cleavage of Z-DEVD-SiR600 resulted in a large bathochromic shift (93 nm) of the absorption maximum and a 432-fold fluorescence enhancement...
  39. doi request reprint Selective two-step labeling of proteins with an off/on fluorescent probe
    Kazuhisa Hirabayashi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 17:14763-71. 2011
    ..This strategy should also be applicable to other tags...
  40. doi request reprint Method for enhancing cell penetration of Gd3+-based MRI contrast agents by conjugation with hydrophobic fluorescent dyes
    Takehiro Yamane
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo, Japan
    Bioconjug Chem 22:2227-36. 2011
    ..Thus, conjugation of hydrophobic fluorescent dyes appears to be an effective approach to improve the cell permeability of Gd(3+) complexes, and should be applicable for further development of Gd(3+)-based MRI contrast agents...
  41. doi request reprint Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 133:5680-2. 2011
    ..This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl...
  42. ncbi request reprint Development of an iminocoumarin-based zinc sensor suitable for ratiometric fluorescence imaging of neuronal zinc
    Kensuke Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    J Am Chem Soc 129:13447-54. 2007
    ..The results indicate that iminocoumarin is a useful fluorophore for fluorescence microscopic imaging and that ZnIC should be useful for studies on the biological functions of Zn2+...
  43. ncbi request reprint Design and synthesis of zinc-selective chelators for extracellular applications
    Eri Kawabata
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:818-9. 2005
    ..This confirms the utility of these new chelatotrs as extracellular Zn2+ chelators for biological applications...
  44. doi request reprint Adipose tissue-derived stem cells inhibit neointimal formation in a paracrine fashion in rat femoral artery
    Masao Takahashi
    Department of Internal Medicine, University of Tokyo, Tokyo, Japan
    Am J Physiol Heart Circ Physiol 298:H415-23. 2010
    ..Our results also suggested that ASCs inhibited neointimal formation via their paracrine effect of stimulation of EC migration in situ rather than the direct integration into the endothelial layer...
  45. doi request reprint Development of enzyme-activated photosensitizer based on intramolecular electron transfer
    Takatoshi Yogo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Bioorg Med Chem Lett 20:4320-3. 2010
    ..TGI-betaGal could selectively ablate lacZ-positive cells, whereas it showed no toxicity to lacZ-negative cells, upon light illumination...
  46. ncbi request reprint Design and synthesis of an enzyme activity-based labeling molecule with fluorescence spectral change
    Toru Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 128:15946-7. 2006
    ..This is the first report of a protein labeling probe which features a change of fluorescence wavelength upon reaction, allowing the labeled protein to be detected even in the presence of unreacted probe...
  47. doi request reprint Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    J Am Chem Soc 134:5029-31. 2012
    ..SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes...
  48. doi request reprint Development of a highly sensitive fluorescence probe for hydrogen peroxide
    Masahiro Abo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 133:10629-37. 2011
    ..7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells...
  49. ncbi request reprint A novel design method of ratiometric fluorescent probes based on fluorescence resonance energy transfer switching by spectral overlap integral
    Hideo Takakusa
    Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Japan
    Chemistry 9:1479-85. 2003
    ....
  50. doi request reprint Development and application of a near-infrared fluorescence probe for oxidative stress based on differential reactivity of linked cyanine dyes
    Daihi Oushiki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 132:2795-801. 2010
    ..We successfully applied this probe to detect ROS produced by HL60 cells and porcine neutrophils and for imaging oxidative stress in a mouse model of peritonitis...
  51. ncbi request reprint Time-resolved long-lived luminescence imaging method employing luminescent lanthanide probes with a new microscopy system
    Kenjiro Hanaoka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    J Am Chem Soc 129:13502-9. 2007
    ..This new imaging technique should facilitate investigations of biological functions with fluorescence microscopy, complementing other fluorescence imaging methodologies...
  52. doi request reprint Development of hypoxia-sensitive Gd3+-based MRI contrast agents
    Shimpei Iwaki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Bioorg Med Chem Lett 22:2798-802. 2012
    ..This enhancement of the r(1) relaxivity could be clearly detected in T(1)-weighted MR images. Thus, 4NO(2)2MeOSAGd is a 'smart' MRI contrast agent for the detection of hypoxia under physiological conditions...
  53. doi request reprint Development of a highly selective fluorescence probe for hydrogen sulfide
    Kiyoshi Sasakura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 133:18003-5. 2011
    ..HSip-1 showed high selectivity and high sensitivity for H(2)S, and its potential for biological applications was confirmed by employing it for fluorescence imaging of H(2)S in live cells...
  54. doi request reprint Development of luciferin analogues bearing an amino group and their application as BRET donors
    Hideo Takakura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Asian J 5:2053-61. 2010
    ..Stable bioluminescence that is not influenced by environmental factors is highly desirable for reliable measurements in biological assays...
  55. ncbi request reprint Evolution of fluorescein as a platform for finely tunable fluorescence probes
    Yasuteru Urano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:4888-94. 2005
    ..The value of this approach is exemplified by its application to develop a novel, highly sensitive, and membrane-permeable fluorescence probe for beta-galactosidase, which is the most widely used reporter enzyme...
  56. doi request reprint Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
    Hideo Takakura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Asian J 6:1800-10. 2011
    ..This generalized design strategy should be applicable to other proteases. Our results indicate that the AL scaffold is appropriate for a range of functional luminophores and represents a useful alternative substrate to luciferin...
  57. doi request reprint Hypoxia-sensitive fluorescent probes for in vivo real-time fluorescence imaging of acute ischemia
    Kazuki Kiyose
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 132:15846-8. 2010
    ..The probes were used for fluorescence imaging of hypoxic cells and real-time monitoring of ischemia in the liver and kidney of live mice...
  58. doi request reprint Red fluorescent probe for monitoring the dynamics of cytoplasmic calcium ions
    Takahiro Egawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033 Japan
    Angew Chem Int Ed Engl 52:3874-7. 2013
    ....
  59. ncbi request reprint Design and synthesis of a novel magnetic resonance imaging contrast agent for selective sensing of zinc ion
    Kenjiro Hanaoka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Biol 9:1027-32. 2002
    ..These complexes should provide the basis for creating a superior Zn(2+)-sensitive MRI contrast agent and are excellent candidates for incorporation into sensors designed for selective detection of Zn(2+) in biological applications...
  60. doi request reprint Rapid cancer detection by topically spraying a γ-glutamyltranspeptidase-activated fluorescent probe
    Yasuteru Urano
    Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Sci Transl Med 3:110ra119. 2011
    ..The gGlu-HMRG probe is practical for clinical application during surgical or endoscopic procedures because of its rapid and strong activation upon contact with GGT on the surface of cancer cells...
  61. doi request reprint A practical strategy to create near-infrared luminescent probes: conversion from fluorescein-based sensors
    Takuya Terai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chem Commun (Camb) 48:2840-2. 2012
    ..As the first demonstration, we present a complex, based on the green-emitting probe DAF-4, that responds to nitric oxide (NO) in aqueous solution with a significant increase in luminescence intensity at 980 nm...
  62. ncbi request reprint Design and synthesis of fluorescent probes for selective detection of highly reactive oxygen species in mitochondria of living cells
    Yuichiro Koide
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo, 113 0033, Japan, CREST, JST, and PRESTO, JST, 4 8 1 Honcho, Kawaguchi, Saitama, 332 0012, Japan
    J Am Chem Soc 129:10324-5. 2007
  63. pmc An enzymatically activated fluorescence probe for targeted tumor imaging
    Mako Kamiya
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 129:3918-29. 2007
    ..This strategy has potential clinical application, for example, in video-assisted laparoscopic tumor resection...
  64. ncbi request reprint A thiol-reactive fluorescence probe based on donor-excited photoinduced electron transfer: key role of ortho substitution
    Takuya Matsumoto
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033 Japan
    Org Lett 9:3375-7. 2007
    ....
  65. doi request reprint Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method
    Toru Komatsu
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Tokyo, 113 0033, Japan
    J Am Chem Soc 133:6745-51. 2011
    ..We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration...
  66. ncbi request reprint Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe
    Yu Gabe
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 126:3357-67. 2004
    ..This strategy should be applicable for the rational design of other novel and potentially useful bioimaging fluorescence probes...
  67. ncbi request reprint Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species
    Ken Ichi Setsukinai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 278:3170-5. 2003
    ..HPF and APF should be useful as tools to study the roles of hROS and (-)OCl in many biological and chemical applications...
  68. doi request reprint In situ evaluation of kinetic resolution catalysts for nitroaldol by rationally designed fluorescence probe
    Takuya Matsumoto
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Org Chem 76:3616-25. 2011
    ..Furthermore, we established an HTS system for asymmetric catalysts, using a fluorescence probe and benzotetramisole. To our knowledge, this is the first fluorescence-based HTS system for asymmetric catalysts...
  69. doi request reprint Angiotensin II and tumor necrosis factor-alpha synergistically promote monocyte chemoattractant protein-1 expression: roles of NF-kappaB, p38, and reactive oxygen species
    Masao Takahashi
    Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    Am J Physiol Heart Circ Physiol 294:H2879-88. 2008
    ..These results also suggest that ANG II and TNF-alpha cooperatively stimulate vascular inflammation in vivo as well as in vitro...
  70. ncbi request reprint Tunable design strategy for fluorescence probes based on 4-substituted BODIPY chromophore: improvement of highly sensitive fluorescence probe for nitric oxide
    Yu Gabe
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Anal Bioanal Chem 386:621-6. 2006
    ..The value of this approach is exemplified by its application to the development of a highly sensitive and pH-independent fluorescence probe for nitric oxide...
  71. doi request reprint A long-lived luminescent probe to sensitively detect arylamine N-acetyltransferase (NAT) activity of cells
    Takuya Terai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Chem Commun (Camb) 48:2234-6. 2012
    ..It is the first NAT probe that is suitable for sensitive, homogeneous, and rapid detection of NAT activity of recombinant enzyme or cell lysate, and is expected to be useful for drug discovery and clinical diagnosis...
  72. doi request reprint Sensitive detection of acrolein in serum using time-resolved luminescence
    Masataka Togashi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Org Lett 12:1704-7. 2010
    ..A novel lanthanide probe was designed, synthesized, and employed for a sensitive and reliable assay of acrolein based on time-resolved luminescence measurement, which suppresses the background signal of serum...
  73. doi request reprint Design and synthesis of a highly sensitive off-on fluorescent chemosensor for zinc ions utilizing internal charge transfer
    Kenjiro Hanaoka
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 16:568-72. 2010
    ..However, 1 is the first Zn(2+)-sensitive off-on fluorescent sensor molecule that employs the ICT mechanism; most off-on sensor molecules for Zn(2+) employ the photoinduced electron transfer (PeT) mechanism...
  74. doi request reprint Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
    Naomi Takiguchi
    Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Pharm Res 28:702-11. 2011
    ..Hydrodynamic injection has been shown to reactivate silenced transgene expression in mouse liver. In this study, the roles of inflammatory cytokines and reactive oxygen species (ROS) in the reactivation were examined...
  75. ncbi request reprint Highly sensitive near-infrared fluorescent probes for nitric oxide and their application to isolated organs
    Eita Sasaki
    Graduate School of Pharmaceutical Sciences, and Faculty of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:3684-5. 2005
    ..They were confirmed to function in isolated intact rat kidneys. Because NIR light can penetrate deeply into tissues, these probes may have potential for in vivo NO imaging...
  76. doi request reprint Design and synthesis of a novel fluorescence probe for Zn2+ based on the spirolactam ring-opening process of rhodamine derivatives
    Hiromi Sasaki
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Bioorg Med Chem 19:1072-8. 2011
    ..Based on the results of this investigation, we selected a candidate xanthene moiety for a Zn(2+) sensor, and successfully developed a new fluorescence probe for Zn(2+)...
  77. doi request reprint A time-resolved fluorescence probe for dipeptidyl peptidase 4 and its application in inhibitor screening
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 16:13479-86. 2010
    ..Interestingly, bestatin did not exhibit DPP4 inhibitory activity. We believe that this screening system will be useful for the discovery of DPP4 inhibitors with novel structural scaffolds...
  78. ncbi request reprint Development of a highly selective fluorescence probe for alkaline phosphatase
    Mitsuyasu Kawaguchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Bioorg Med Chem Lett 21:5088-91. 2011
    ..TG-mPhos should enable precise fluorescence imaging of ALP activity in biological applications...
  79. ncbi request reprint Development of a highly specific rhodamine-based fluorescence probe for hypochlorous acid and its application to real-time imaging of phagocytosis
    Suguru Kenmoku
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 129:7313-8. 2007
    ..HySOx is tolerant to autoxidation and photobleaching under bioimaging conditions. Regulation of the spirocyclization of rhodamines, as we describe here, provides a new approach to the rational development of novel fluorescence probes...
  80. ncbi request reprint Mechanism-based molecular design of highly selective fluorescence probes for nitrative stress
    Tasuku Ueno
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 128:10640-1. 2006
    ..NiSPYs should be potentially useful as tools to study the role of nitrative stress in various biological applications...
  81. ncbi request reprint Modulation of luminescence intensity of lanthanide complexes by photoinduced electron transfer and its application to a long-lived protease probe
    Takuya Terai
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 128:6938-46. 2006
    ..This probe, combined with time-resolved measurements, was confirmed in model experiments to be useful for the screening of inhibitors, as well as for clinical diagnosis...
  82. ncbi request reprint Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer
    Tomoko Mineno
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Org Lett 8:5963-6. 2006
    ..These novel dye derivatives display high quantum yields and are expected to be useful as labeling agents...
  83. ncbi request reprint Zinc sensing for cellular application
    Kazuya Kikuchi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Curr Opin Chem Biol 8:182-91. 2004
    ..Some of the biological functions of Zn2+ have been clarified using these sensor molecules, especially in neuronal cells, which contain a high concentration of free Zn2+...
  84. doi request reprint Rational design of ratiometric near-infrared fluorescent pH probes with various pKa values, based on aminocyanine
    Takuya Myochin
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 133:3401-9. 2011
    ..This design strategy is very simple and flexible, and should be applicable to develop NIR pH probes for various applications...
  85. doi request reprint Fluorescence imaging of tumors with "smart" pH-activatable targeted probes
    Daisuke Asanuma
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Methods Mol Biol 574:47-62. 2009
    ....
  86. ncbi request reprint Development of selective, visible light-excitable, fluorescent magnesium ion probes with a novel fluorescence switching mechanism
    Takuji Shoda
    The Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Analyst 128:719-23. 2003
    ..8. The pKa value of the Mg2+-2'-CF complex was 6.8, and the fluorescence intensity was increased in the neutral conditions. Thus, the addition of Mg2+ resulted in a lowering of the pKa, and also an increase of the fluorescence intensity...
  87. ncbi request reprint Spatiotemporal laser inactivation of inositol 1,4,5-trisphosphate receptors using synthetic small-molecule probes
    Takanari Inoue
    Graduate School of Pharmaceutical Sciences, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    Chem Biol 10:503-9. 2003
    ..Such real-time inactivation of IP(3)R only within a subcellular region may provide a powerful method for investigating spatiotemporal dynamics of Ca(2+) signaling...
  88. ncbi request reprint A novel, cell-permeable, fluorescent probe for ratiometric imaging of zinc ion
    Satoko Maruyama
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 124:10650-1. 2002
    ..Using these probes, changes of intracellular Zn(2+) concentration in cultured cells were monitored successfully. We believe that these probes will be extremely useful in studies on the biological functions of Zn(2+)...
  89. ncbi request reprint Highly activatable and rapidly releasable caged fluorescein derivatives
    Tomonori Kobayashi
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 129:6696-7. 2007
  90. ncbi request reprint Highly efficient and photostable photosensitizer based on BODIPY chromophore
    Takatoshi Yogo
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 127:12162-3. 2005
    ..HeLa cells loaded with 2I-BDP could be photosensitized by light illumination, demonstrating that 2I-BDP is potentially useful as a reagent for cell photosensitization, oxidative stress studies, or PDT...
  91. ncbi request reprint Improvement and biological applications of fluorescent probes for zinc, ZnAFs
    Tomoya Hirano
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 124:6555-62. 2002
    ..Using ZnAF-2F DA, we could measure the changes of intracellular Zn2+ in cultured cells and hippocampal slices...
  92. ncbi request reprint Extension of the applicable range of fluorescein: a fluorescein-based probe for Western blot analysis
    Mako Kamiya
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Angew Chem Int Ed Engl 44:5439-41. 2005
  93. doi request reprint Evaluation of characteristic deuterium distributions of ephedrines and methamphetamines by NMR spectroscopy for drug profiling
    Teruki Matsumoto
    National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo, Japan
    Anal Chem 80:1176-81. 2008
    ..This isotope ratio analysis method should be useful to trace the origins of seized methamphetamine in Southeast Asia...
  94. ncbi request reprint Iron hydroxide nanoparticles coated with poly(ethylene glycol)-poly(aspartic acid) block copolymer as novel magnetic resonance contrast agents for in vivo cancer imaging
    Michiaki Kumagai
    Department of Materials Engineering, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Colloids Surf B Biointerfaces 56:174-81. 2007
    ....
  95. ncbi request reprint Orthogonality of calcium concentration and ability of 4,5-diaminofluorescein to detect NO
    Noriyuki Suzuki
    Graduate School of Pharmaceutical Sciences, Faculty of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 277:47-9. 2002
    ..Therefore it is concluded that DAF-2 can provide reliable information on NO production in biological systems regardless of the dynamic changes of Ca2+ concentration...
  96. ncbi request reprint [Mechanism-based molecular design of highly selective peroxynitrite probe]
    Tasuku Ueno
    Tanpakushitsu Kakusan Koso 52:1540-5. 2007
  97. ncbi request reprint [Cellular imaging by using molecular probes]
    Hirotatsu Kojima
    Nihon Yakurigaku Zasshi 132:7-10. 2008
  98. ncbi request reprint [In-vivo cancer fluorescence imaging with novel precisely-designed fluorescence probes]
    Yasuteru Urano
    Tanpakushitsu Kakusan Koso 52:1594-600. 2007
  99. ncbi request reprint [Development of fluorescent probes for nitric oxide]
    Hirotatsu Kojima
    Tanpakushitsu Kakusan Koso 52:1534-9. 2007
  100. ncbi request reprint [Analysis of intracellular membrane protein function by small molecule-based chromophore-assisted laser inactivation]
    Kazuya Kikuchi
    Tanpakushitsu Kakusan Koso 50:1388-94. 2005
  101. ncbi request reprint Design and synthesis of an enzyme-cleavable sensor molecule for phosphodiesterase activity based on fluorescence resonance energy transfer
    Hideo Takakusa
    Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Am Chem Soc 124:1653-7. 2002
    ..The method described in this paper for designing enzyme-cleavable sensor molecules based on FRET should be readily applicable to other hydrolytic enzymes...