Hozumi Motohashi

Summary

Affiliation: University of Tsukuba
Country: Japan

Publications

  1. pmc MafG sumoylation is required for active transcriptional repression
    Hozumi Motohashi
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 26:4652-63. 2006
  2. pmc Genetic evidence that small maf proteins are essential for the activation of antioxidant response element-dependent genes
    Fumiki Katsuoka
    Center for TARA, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 25:8044-51. 2005
  3. ncbi request reprint Nrf2 transcriptionally activates the mafG gene through an antioxidant response element
    Fumiki Katsuoka
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
    J Biol Chem 280:4483-90. 2005
  4. ncbi request reprint Molecular basis distinguishing the DNA binding profile of Nrf2-Maf heterodimer from that of Maf homodimer
    Momoko Kimura
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8572, Japan
    J Biol Chem 282:33681-90. 2007
  5. pmc Small Maf proteins serve as transcriptional cofactors for keratinocyte differentiation in the Keap1-Nrf2 regulatory pathway
    Hozumi Motohashi
    Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8577, Japan
    Proc Natl Acad Sci U S A 101:6379-84. 2004
  6. pmc Constitutive expression of aryl hydrocarbon receptor in keratinocytes causes inflammatory skin lesions
    Masafumi Tauchi
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance TARA, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 25:9360-8. 2005
  7. ncbi request reprint Inducibility of cytochrome P450 1A1 and chemical carcinogenesis by benzo[a]pyrene in AhR repressor-deficient mice
    Tomonori Hosoya
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 365:562-7. 2008
  8. pmc Transgene insertion in proximity to the c-myb gene disrupts erythroid-megakaryocytic lineage bifurcation
    Harumi Y Mukai
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8577, Japan
    Mol Cell Biol 26:7953-65. 2006
  9. ncbi request reprint Nrf2-Keap1 defines a physiologically important stress response mechanism
    Hozumi Motohashi
    Center for Tsukuba Advanced Research Alliance, Exploratory Research for Advanced Technology Japan Science and Technology Corporation, University of Tsukuba, Tsukuba 305 8577, Japan
    Trends Mol Med 10:549-57. 2004
  10. ncbi request reprint Hepatocyte-specific deletion of the keap1 gene activates Nrf2 and confers potent resistance against acute drug toxicity
    Hiromi Okawa
    Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 339:79-88. 2006

Collaborators

Detail Information

Publications28

  1. pmc MafG sumoylation is required for active transcriptional repression
    Hozumi Motohashi
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 26:4652-63. 2006
    ....
  2. pmc Genetic evidence that small maf proteins are essential for the activation of antioxidant response element-dependent genes
    Fumiki Katsuoka
    Center for TARA, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 25:8044-51. 2005
    ..These data explicitly demonstrate that small Mafs play critical roles in the inducible expression of a significant portion of ARE-dependent genes...
  3. ncbi request reprint Nrf2 transcriptionally activates the mafG gene through an antioxidant response element
    Fumiki Katsuoka
    Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305 8577, Japan
    J Biol Chem 280:4483-90. 2005
    ..These results demonstrate that mafG is itself an ARE-dependent gene that is regulated by an Nrf2/small Maf heterodimer and suggest the presence of an autoregulatory feedback pathway for mafG transcriptional regulation...
  4. ncbi request reprint Molecular basis distinguishing the DNA binding profile of Nrf2-Maf heterodimer from that of Maf homodimer
    Momoko Kimura
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8572, Japan
    J Biol Chem 282:33681-90. 2007
    ..Our contention is supported that the differential DNA binding specificity between Maf homodimers and Nrf2-Maf heterodimers establishes the differential gene regulation by these dimer-forming transcription factors...
  5. pmc Small Maf proteins serve as transcriptional cofactors for keratinocyte differentiation in the Keap1-Nrf2 regulatory pathway
    Hozumi Motohashi
    Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8577, Japan
    Proc Natl Acad Sci U S A 101:6379-84. 2004
    ..This rescue phenotype of mafG::mafF::keap1 triple compound mutant mice phenocopies that of the nrf2::keap1 compound mutant mice, indicating that the small Maf proteins MafG and MafF must functionally cooperate with Nrf2 in vivo...
  6. pmc Constitutive expression of aryl hydrocarbon receptor in keratinocytes causes inflammatory skin lesions
    Masafumi Tauchi
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance TARA, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Mol Cell Biol 25:9360-8. 2005
    ..We demonstrate that constitutive activation of the AhR pathway causes inflammatory skin lesions and suggests a new mechanism for the exacerbation of inflammatory diseases after exposure to occupational and environmental xenobiotics...
  7. ncbi request reprint Inducibility of cytochrome P450 1A1 and chemical carcinogenesis by benzo[a]pyrene in AhR repressor-deficient mice
    Tomonori Hosoya
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 365:562-7. 2008
    ....
  8. pmc Transgene insertion in proximity to the c-myb gene disrupts erythroid-megakaryocytic lineage bifurcation
    Harumi Y Mukai
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8577, Japan
    Mol Cell Biol 26:7953-65. 2006
    ....
  9. ncbi request reprint Nrf2-Keap1 defines a physiologically important stress response mechanism
    Hozumi Motohashi
    Center for Tsukuba Advanced Research Alliance, Exploratory Research for Advanced Technology Japan Science and Technology Corporation, University of Tsukuba, Tsukuba 305 8577, Japan
    Trends Mol Med 10:549-57. 2004
    ..Furthermore, extensive studies have suggested that the Nrf2-Keap1 system contributes to protection against various pathologies, including carcinogenesis, liver toxicity, respiratory distress and inflammation...
  10. ncbi request reprint Hepatocyte-specific deletion of the keap1 gene activates Nrf2 and confers potent resistance against acute drug toxicity
    Hiromi Okawa
    Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 339:79-88. 2006
    ..These results demonstrate that chronic activation of Nrf2 confers animals with resistance to xenobiotics without affecting the morphological and physiological integrity of hepatocytes...
  11. ncbi request reprint Predictive base substitution rules that determine the binding and transcriptional specificity of Maf recognition elements
    Tae Yamamoto
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Genes Cells 11:575-91. 2006
    ..This study thus manifests that a clear set of rules pertaining to the cis-acting element determine whether a given MARE preferentially associates with MafG homodimer or with MafG:Nrf2 heterodimer...
  12. ncbi request reprint Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes
    Keiko Nohara
    Environmental Health Sciences Division, National Institute for Environmental Studies, University of Tsukuba, Tsukuba, Japan
    J Immunol 174:2770-7. 2005
    ..They also indicate that AhR activation in T cells and not in B cells suppresses the immunization-induced increase in both T cells and B cells...
  13. ncbi request reprint Deletion of the selenocysteine tRNA gene in macrophages and liver results in compensatory gene induction of cytoprotective enzymes by Nrf2
    Takafumi Suzuki
    Exploratory Research for Advanced Technology Environmental Response Project, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Japan
    J Biol Chem 283:2021-30. 2008
    ..Our results thus demonstrate that reduced selenoprotein activity is counterbalanced by an Nrf2-mediated cytoprotective response, which is essential for maintaining cellular redox homeostasis and viability...
  14. pmc Transforming growth factor-β induces transcription factors MafK and Bach1 to suppress expression of the heme oxygenase-1 gene
    Yukari Okita
    Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    J Biol Chem 288:20658-67. 2013
    ..As TGF-β is activated after tissue injury and in the process of cancer development, these findings suggest a novel mechanism by which damaged tissue becomes vulnerable to oxidative stress and xenobiotics. ..
  15. ncbi request reprint Identification of polymorphisms in the promoter region of the human NRF2 gene
    Tae Yamamoto
    Graduate School of Comprehensive Human Sciences, Majors of Medical Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 321:72-9. 2004
    ..However, available lines of evidence suggest the importance of examining the link between NRF2 polymorphisms and other oxidative stress-related diseases...
  16. pmc Small Maf compound mutants display central nervous system neuronal degeneration, aberrant transcription, and Bach protein mislocalization coincident with myoclonus and abnormal startle response
    Fumiki Katsuoka
    Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba 305 8577, Japan
    Mol Cell Biol 23:1163-74. 2003
    ..Thus compound mafG::mafK mutants develop age- and maf gene dosage-dependent cell-autonomous neuronal deficiencies that lead to profound neurological defects...
  17. ncbi request reprint Keap1-null mutation leads to postnatal lethality due to constitutive Nrf2 activation
    Nobunao Wakabayashi
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Nat Genet 35:238-45. 2003
    ..Breeding to Nrf2-deficient mice reversed the phenotypic Keap1 deficiencies. These experiments show that Keap1 acts upstream of Nrf2 in the cellular response to oxidative and xenobiotic stress...
  18. ncbi request reprint Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels
    Norio Suzuki
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Blood 102:3575-83. 2003
    ..This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues...
  19. ncbi request reprint [Small Maf proteins as transcription factors regulating maturation and maintenance of the cell]
    Hozumi Motohashi
    Center for Tsukuba Advanced Research Alliance, Institute of Basic Medical Sciences, University of Tsukuba, Tennoudai 1 1 1, Tsukuba 305 8577, Japan
    Seikagaku 75:1193-201. 2003
  20. pmc Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse
    Takashi Moriguchi
    Institute of Basic Medical Sciences, Center for Tsukuba Advanced Research Alliance, and Exploratory Research for Advanced Technology Environmental Response Project, University of Tsukuba, Tsukuba 305 8575, Japan
    Proc Natl Acad Sci U S A 100:5652-7. 2003
    ..Thus, the hAHR knock-in mouse is a humanized model mouse that may better predict the biological effects of bioaccumulative environmental toxicants like TCDD in humans...
  21. pmc Nrf2 Neh5 domain is differentially utilized in the transactivation of cytoprotective genes
    Jianyong Zhang
    Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Biochem J 404:459-66. 2007
    ..Taken together, these results indicate that the Neh5 domain has the ability to regulate Nrf2 target gene transcription, yet the role of the Neh5 domain in transcription varies from gene to gene...
  22. ncbi request reprint Integration and diversity of the regulatory network composed of Maf and CNC families of transcription factors
    Hozumi Motohashi
    Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 305 8577, Tsukuba, Japan
    Gene 294:1-12. 2002
    ..Deciphering the molecular basis of the regulatory network composed of Maf and CNC families of transcription factors will undoubtedly lead to a new paradigm for the cooperative function of transcription factors...
  23. ncbi request reprint Carcinogenesis and transcriptional regulation through Maf recognition elements
    Hozumi Motohashi
    Graduate School of Comprehensive Human Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Cancer Sci 98:135-9. 2007
    ..On the contrary, the constitutive activation of Nrf2 due to mutations in the keap1 gene is characteristically observed in lung cancer cells, suggesting that induced expression of Nrf2 target genes favors the prevalence of cancer cells...
  24. ncbi request reprint Gene expression of detoxifying enzymes in AhR and Nrf2 compound null mutant mouse
    Shuhei Noda
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba 305 8577, Japan
    Biochem Biophys Res Commun 303:105-11. 2003
    ..Here, we report an initial characterization of the AhR-Nrf2 double mutant mice, which may serve as a simplified bioassay system to evaluate xenobiotic toxicity and metabolic biotransformation of various drugs and environmental chemicals...
  25. ncbi request reprint Solution structure of the DNA-binding domain of MafG
    Hideki Kusunoki
    Institute of Basic Medical Science, University of Tsukuba, Tsukuba, Ibaraki 305 8575 Japan
    Nat Struct Biol 9:252-6. 2002
    ..The structural similarity between MafG and Skn-1 enables us to propose a possible mechanism by which Maf family proteins recognize their consensus DNA sequences...
  26. ncbi request reprint Modulation of gene expression by cancer chemopreventive dithiolethiones through the Keap1-Nrf2 pathway. Identification of novel gene clusters for cell survival
    Mi Kyoung Kwak
    Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 278:8135-45. 2003
    ....
  27. ncbi request reprint Evaluation of MafG interaction with Maf recognition element arrays by surface plasmon resonance imaging technique
    Motoki Kyo
    TOYOBO Co Ltd Bio 21 Project, 10 24 Toyo Cho, Tsuruga, Fukui 914 0047, Japan
    Genes Cells 9:153-64. 2004
    ..These results demonstrate that the double-stranded DNA array fabricated with the modified multistep procedure can be applied for the comprehensive analysis of the transcription factor-DNA interaction...
  28. pmc Physiological significance of reactive cysteine residues of Keap1 in determining Nrf2 activity
    Tae Yamamoto
    Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai 980 8575, Japan
    Mol Cell Biol 28:2758-70. 2008
    ..These results demonstrate critical roles of the cysteine residues in vivo in maintaining Keap1 function, such that Nrf2 is repressed under quiescent conditions and active in response to oxidants/electrophiles...