Takumi Misaka

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. pmc Establishment of a new cell-based assay to measure the activity of sweeteners in fluorescent food extracts
    Yasuka Toda
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Agric Food Chem 59:12131-8. 2011
  2. pmc Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16
    Takanobu Sakurai
    General Research Institute of Food Science and Technology, Nissin Foods Holdings Co, Ltd, Shiga 525 0058, Japan
    J Biol Chem 285:28373-8. 2010
  3. pmc The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs
    Sho Ishii
    Central Research Institute, Mizkan Group Co, Handa, Aichi, Japan
    FEBS J 279:1857-70. 2012
  4. doi request reprint Molecular mechanisms of the action of miraculin, a taste-modifying protein
    Takumi Misaka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Semin Cell Dev Biol 24:222-5. 2013
  5. doi request reprint Wheat cysteine proteases triticain alpha, beta and gamma exhibit mutually distinct responses to gibberellin in germinating seeds
    Toshihiro Kiyosaki
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    J Plant Physiol 166:101-6. 2009
  6. doi request reprint Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FASEB J 22:2323-30. 2008
  7. ncbi request reprint Neoculin, a taste-modifying protein, is recognized by human sweet taste receptor
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    Neuroreport 17:1241-4. 2006
  8. doi request reprint pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances
    Takanobu Sakurai
    General Research Institute of Food Science and Technology, Nissin Foods Holdings Co, Ltd, Kusatsu shi, Shiga, Japan
    J Agric Food Chem 57:2508-14. 2009
  9. ncbi request reprint Microbial production of sensory-active miraculin
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 360:407-11. 2007
  10. pmc Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan
    PLoS ONE 6:e19448. 2011

Collaborators

Detail Information

Publications53

  1. pmc Establishment of a new cell-based assay to measure the activity of sweeteners in fluorescent food extracts
    Yasuka Toda
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Agric Food Chem 59:12131-8. 2011
    ..This luminescence-based assay will be a powerful tool to objectively evaluate the sweetness of food-derived samples even at an industry level...
  2. pmc Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16
    Takanobu Sakurai
    General Research Institute of Food Science and Technology, Nissin Foods Holdings Co, Ltd, Shiga 525 0058, Japan
    J Biol Chem 285:28373-8. 2010
    ..This study characterizes the molecular interaction between hTAS2R16 and beta-D-glucopyranoside and will also facilitate rational design of bitter blockers...
  3. pmc The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs
    Sho Ishii
    Central Research Institute, Mizkan Group Co, Handa, Aichi, Japan
    FEBS J 279:1857-70. 2012
    ..5 μm. Capsaicin and its analogs are thus useful tools for physiological analysis of PKD1L3/PKD2L1 function...
  4. doi request reprint Molecular mechanisms of the action of miraculin, a taste-modifying protein
    Takumi Misaka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Semin Cell Dev Biol 24:222-5. 2013
    ..Since sweet-tasting proteins may be used as low-calorie sweeteners because they contain almost no calories, it is expected that MCL will be used in the near future as a new low-calorie sweetener or to modify the taste of sour fruits...
  5. doi request reprint Wheat cysteine proteases triticain alpha, beta and gamma exhibit mutually distinct responses to gibberellin in germinating seeds
    Toshihiro Kiyosaki
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    J Plant Physiol 166:101-6. 2009
    ..These results suggest that triticain alpha, beta and gamma play differential roles in seed maturation as well as in digestion of storage proteins during germination...
  6. doi request reprint Acid-induced sweetness of neoculin is ascribed to its pH-dependent agonistic-antagonistic interaction with human sweet taste receptor
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FASEB J 22:2323-30. 2008
    ..As a result, NCL competitively inhibits the variant-induced receptor activation. All these data suggest that NCL acts as an hT1R2-hT1R3 agonist at acidic pH but functionally changes into its antagonist at neutral pH...
  7. ncbi request reprint Neoculin, a taste-modifying protein, is recognized by human sweet taste receptor
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    Neuroreport 17:1241-4. 2006
    ..The sweetness was also suppressed with the addition of lactisole. These results suggest that both the sweetness and the taste-modifying activity are mediated via the human sweet taste receptor...
  8. doi request reprint pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances
    Takanobu Sakurai
    General Research Institute of Food Science and Technology, Nissin Foods Holdings Co, Ltd, Kusatsu shi, Shiga, Japan
    J Agric Food Chem 57:2508-14. 2009
    ..Our results suggest that the inhibition of the bitter taste receptors can be attributed to the bitterness-masking effect of the acidic dipeptides and that acidic pH may be one of the critical factors responsible for this sensory event...
  9. ncbi request reprint Microbial production of sensory-active miraculin
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 360:407-11. 2007
    ..oryzae-mediated expression system. Both mutants found to have lost the taste-modifying activity. The result suggests that the histidine-30 residue is important for the taste-modifying activity of MCL...
  10. pmc Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan
    PLoS ONE 6:e19448. 2011
    ..These findings are important for understanding the pH-sensitive functional changes in proteinaceous ligands in general and the interaction of taste receptor-taste substance in particular...
  11. doi request reprint Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochim Biophys Acta 1800:986-92. 2010
    ..Miraculin (MCL) is a taste-modifying protein that converts sourness into sweetness. The molecular mechanism underlying the taste-modifying action of MCL is unknown...
  12. ncbi request reprint Non-acidic compounds induce the intense sweet taste of neoculin, a taste-modifying protein
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 75:1600-2. 2011
    ..We report that ammonium chloride and certain amino acids elicit the intense sweetness of neoculin. Neoculin can thus sweeten amino acid-enriched foods...
  13. doi request reprint Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo, 113 8657, Japan
    Biotechnol Lett 33:103-7. 2011
    ..The CS shuffling can be performed using a small library and within one week, and is an effective screening strategy of soluble protein expression...
  14. doi request reprint Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae
    Taishi Sugawara
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 382:704-10. 2009
    ..Fluorescence size-exclusion chromatography showed that hTAS2R41 exhibited monodispersity in DDM/CHS without aggregates, suggesting that hTAS2R41 is a good target for future crystallization trials...
  15. ncbi request reprint Transgenic labeling of taste receptor cells in model fish under the control of the 5'-upstream region of medaka phospholipase C-beta 2 gene
    Yoshiko Aihara
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Gene Expr Patterns 7:149-57. 2007
    ..This is the first report of successful labeling of taste receptor cells in two model fish species under the control of the plc-beta2 promoter. This promoter will be a useful genetic tool to study the vertebrate taste system in general...
  16. pmc Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae
    Ken ichiro Nakajima
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Appl Environ Microbiol 72:3716-23. 2006
    ..The original sweetness and taste-modifying activity of rNCL were comparable to those of native NCL when confirmed by calcium imaging with human embryonic kidney cells expressing the human sweet taste receptor and by sensory tests...
  17. pmc Human sweet taste receptor mediates acid-induced sweetness of miraculin
    Ayako Koizumi
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 108:16819-24. 2011
    ..Our data suggest that MCL binds hT1R2-hT1R3 as an antagonist at neutral pH and functionally changes into an agonist at acidic pH, and we conclude this may cause its taste-modifying activity...
  18. ncbi request reprint Characterization of ligands for fish taste receptors
    Hideaki Oike
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Neurosci 27:5584-92. 2007
    ..These results suggest that, although there may be some fish-specific way of discriminating ligands, vertebrates could have a conserved gustatory mechanism by which T1Rs and T2Rs respond to attractive and aversive tastants, respectively...
  19. doi request reprint The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides
    Takanobu Sakurai
    General Research Institute of Food Science and Technology, Nissin Foods Holdings Co Ltd, Shiga, Japan
    Biochem Biophys Res Commun 402:595-601. 2010
    ..As a result, these anomers elicit different taste sensations. Mutational analysis of hTAS2R16 also indicated that gentiobiose and β-D-glucopyranosides, such as salicin share a common binding site of hTAS2R16...
  20. ncbi request reprint Polyphenols in alcoholic beverages activating constitutive androstane receptor CAR
    Ruiqing Yao
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Biosci Biotechnol Biochem 75:1635-7. 2011
    ..Since CAR regulates many genes involved in energy metabolism, the possibility exists that these polyphenols would reduce the risk of certain alcohol-induced metabolic disorders with the help of CAR...
  21. ncbi request reprint pH-Dependent structural change in neoculin with special reference to its taste-modifying activity
    Yuji Morita
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:2552-5. 2009
    ..These results suggest that the sweetness of neoculin depends on structural change accompanying the pH change, with the histidine residues playing a key role...
  22. pmc Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae
    Yoshiro Ishimaru
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FASEB J 24:4058-67. 2010
    ....
  23. doi request reprint Biochemical and genomic analysis of neoculin compared to monocot mannose-binding lectins
    Akiko Shimizu-Ibuka
    Department of Applied Biological Chemistry and Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Agric Food Chem 56:5338-44. 2008
    ..These data strongly suggest that neoculin has no lectin-like properties, encouraging its practical use in the food industry...
  24. ncbi request reprint Signal peptide peptidase and its homologs in Arabidopsis thaliana--plant tissue-specific expression and distinct subcellular localization
    Tomoko Tamura
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    FEBS J 275:34-43. 2008
    ..These results suggest that AtSPP mediates the cleavage of signal peptide in the ER membrane as well as HsSPP does, and also that AtSPPL1 and AtSPPL2 located in the endosomes have distinct roles in cells...
  25. doi request reprint Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model
    Motohiro Miyano
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1, Yayoi, Bunkyo ku, Tokyo, 113 8657, Japan
    J Agric Food Chem 58:11870-5. 2010
    ..This device made it possible to detect the interaction with lipid bilayer and dissected the mechanism of taste continuity...
  26. ncbi request reprint Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain
    Ayako Koizumi
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Tokyo, Japan
    Biochem Biophys Res Commun 358:585-9. 2007
    ..Although T1R2-T1R3 is known to have multiple potential ligand-binding sites to receive a wide variety of sweeteners, the present study is apparently the first to identify the ATD of hT1R3 as a new sweetener-binding region...
  27. ncbi request reprint Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum aestivum L., specifically digests gliadin and is regulated by intrinsic cystatins
    Toshihiro Kiyosaki
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    FEBS J 274:1908-17. 2007
    ..These values are comparable with those found for GST-progliadain inhibition by E-64 and egg-white cystatin, and are consistent with the possibility that, in germinating wheat seeds, gliadain is under the control of intrinsic cystatins...
  28. ncbi request reprint Transient receptor potential channel M5 and phospholipaseC-beta2 colocalizing in zebrafish taste receptor cells
    Yuki Yoshida
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Neuroreport 18:1517-20. 2007
    ..These results demonstrate that zfTRPM5 and PLC-beta2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors...
  29. doi request reprint Neuron differentiation-related genes are up-regulated in the hypothalamus of odorant-inhaling rats subjected to acute restraint stress
    Akio Nakamura
    Technical Research Institute R and D Center, T Hasegawa Company, Ltd, 29 7 Kariyado, Nakahara ku, Kawasaki shi 211 0022, Japan
    J Agric Food Chem 58:7922-9. 2010
    ....
  30. doi request reprint The single pore residue Asp523 in PKD2L1 determines Ca2+ permeation of the PKD1L3/PKD2L1 complex
    Chisato Fujimoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 404:946-51. 2011
    ..These results demonstrate that Asp(523) in PKD2L1 is a key determinant of Ca(2+) permeation into the PKD1L3/PKD2L1 complex and that PKD2L1 contributes to forming the pore of the PKD1L3/PKD2L1 channel...
  31. ncbi request reprint Human bitter taste receptors hTAS2R8 and hTAS2R39 with differential functions to recognize bitter peptides
    Yohei Ueno
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 75:1188-90. 2011
    ..The partial structure of Pro-Arg activated hTAS2R39, but did not activate hTAS2R8. These receptors may not indiscriminately recognize bitter peptides, but have a differential function in their recognition...
  32. doi request reprint Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish
    Takashi Ieki
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113 8657, Japan
    J Comp Neurol 521:1781-802. 2013
    ..These results provide insight into the basic neuronal architecture of gustatory information processing that is common among vertebrates...
  33. doi request reprint Acetic acid activates PKD1L3-PKD2L1 channel--a candidate sour taste receptor
    Sho Ishii
    Central Research Institute, Mizkan Group Co, 2 6 Nakamura cho, Handa, Aichi 475 8585, Japan
    Biochem Biophys Res Commun 385:346-50. 2009
    ..This method revealed that PKD1L3-PKD2L1 channel activation by acetic acid was pH-dependent and occurred when the ambient pH was <3.1...
  34. ncbi request reprint Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting
    Kaede Terauchi
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Plant Physiol 163:856-62. 2006
    ..These observations indicated that, in the case of soyAP1, the PSI is not involved in vacuolar targeting, also suggesting that the function of the PSI differs depending on its origin...
  35. pmc Two distinct determinants of ligand specificity in T1R1/T1R3 (the umami taste receptor)
    Yasuka Toda
    From the Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    J Biol Chem 288:36863-77. 2013
    ..A complex molecular mechanism involving changes in the properties of both the orthosteric and non-orthosteric sites of T1R1 underlies the determination of ligand specificity in mammalian T1R1/T1R3...
  36. pmc Nuclear receptor-mediated alleviation of alcoholic fatty liver by polyphenols contained in alcoholic beverages
    Ruiqing Yao
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    PLoS ONE 9:e87142. 2014
    ..In addition, there was no segregation of the gene expression profiles among these groups. These results provide a molecular basis for the prevention of alcohol-induced stress by the polyphenols in alcoholic beverages. ..
  37. ncbi request reprint The use of mammalian cultured cells loaded with a fluorescent dye shows specific membrane penetration of undissociated acetic acid
    Sho Ishii
    Central Research Institute, Mizkan Group Co, Handa, Aichi, Japan
    Biosci Biotechnol Biochem 76:523-9. 2012
    ..The method used in this study might be convenient in evaluating the intracellular acidification of cultured cells by acids in the external environment...
  38. ncbi request reprint Differential expression analysis throughout the weaning period in the mouse cerebral cortex
    Naohiro Maeda
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 431:437-43. 2013
    ..These data suggest that the novel experience of food intake modulates gene expression profiles in the murine cerebral cortex at the weaning stage...
  39. doi request reprint Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae
    Keisuke Ito
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 371:841-5. 2008
    ..Thus, we have demonstrated a technique allowing the expression of mutant membrane proteins within 5 days, combining a GFP-fusion expression system and yeast homologous recombination...
  40. ncbi request reprint Arachidonic acid can function as a signaling modulator by activating the TRPM5 cation channel in taste receptor cells
    Hideaki Oike
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Biochim Biophys Acta 1761:1078-84. 2006
    ..These results strongly suggest the possibility that arachidonic acid acts as a modulator of TRPM5 in taste signaling pathways...
  41. doi request reprint Aquaporin-11 knockout mice and polycystic kidney disease animals share a common mechanism of cyst formation
    Shinji Okada
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    FASEB J 22:3672-84. 2008
    ..We also demonstrated the activation of apoptosis via the ER-stress pathway in the proximal tubule cells with vacuolized ER. These results provide new insights into the physiological roles of AQP11...
  42. doi request reprint Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor
    Takamasa Imada
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 397:220-5. 2010
    ..Our results strongly indicate that amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor and also that this receptor has multiple inhibitor binding sites...
  43. pmc Artepillin C, a major ingredient of Brazilian propolis, induces a pungent taste by activating TRPA1 channels
    Taketoshi Hata
    Nagaragawa Research Center, API Co, Ltd, Nagara, Gifu, Japan
    PLoS ONE 7:e48072. 2012
    ..These findings strongly indicate that artepillin C is the main pungent ingredient in EEBP and stimulates a pungent taste by activating TRPA1 channels...
  44. ncbi request reprint The hepatic genes for immunoproteasome are upregulated by refeeding after fasting in the rat
    Shota Ushiama
    Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 74:1320-3. 2010
    ..In addition, refeeding induced upregulation of the genes encoding immunoproteasome components. Finally, immunoblot analysis confirmed changes in protein levels, suggesting that refeeding after fasting enhanced immune function...
  45. doi request reprint Dietary zinc status reversibly alters both the feeding behaviors of the rats and gene expression patterns in diencephalon
    Shinji Okada
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Japan
    Biofactors 38:203-18. 2012
    ..These results show that feeding behaviors, taste preferences and gene expression patterns in the diencephalon respond quickly to changing zinc levels...
  46. pmc Dietary flavonoids activate the constitutive androstane receptor (CAR)
    Ruiquing Yao
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Agric Food Chem 58:2168-73. 2010
    ..A significant increase in cyp2b10 mRNA content was observed only in wild-type mice fed chrysin. These results suggest that dietary flavonoids regulate CAR activity and thereby accelerate both detoxification and energy metabolism...
  47. ncbi request reprint L-Theanine elicits umami taste via the T1R1 + T1R3 umami taste receptor
    Masataka Narukawa
    Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, 113 8657, Japan
    Amino Acids 46:1583-7. 2014
    ..The site-directed mutagenesis analysis revealed that L-theanine binds to L-amino acid binding site in the Venus flytrap domain of T1R1. This study shows that L-theanine elicits an umami taste via T1R1 + T1R3...
  48. ncbi request reprint Taste receptor cells express voltage-dependent potassium channels in a cell age-specific manner
    Makoto Ohmoto
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Chem Senses 31:739-46. 2006
    ..These results suggest that taste bud cells other than beta2/M5-TRCs are depolarized by some stimuli and also that beta2/M5-TRCs have cell age-dependent molecular mechanisms of repolarization...
  49. doi request reprint Protective role of the leukotriene B4 receptor BLT2 in murine inflammatory colitis
    Yoshiko Iizuka
    Department of Medical Biochemistry, Graduate School of Medical Sciences, The University of Tokyo, Tokyo, Japan
    FASEB J 24:4678-90. 2010
    ..These novel results suggest a direct anti-inflammatory role of BLT2 that is distinct from the proinflammatory roles of BLT1...
  50. ncbi request reprint Development of a cultured cell-based human-taste evaluation system
    Takumi Misaka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    Biosci Biotechnol Biochem 77:1613-6. 2013
    ..Moreover, such an evaluation system should be also useful in advancing our understanding of the ways tastants are recognized by taste receptors. ..
  51. ncbi request reprint Differential expression of wheat aspartic proteinases, WAP1 and WAP2, in germinating and maturing seeds
    Tomoko Tamura
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    J Plant Physiol 164:470-7. 2007
    ..WAP1 and WAP2 were expressed widely in germinating and maturing seeds. Such diversity in site- and stage-specific expression of the two enzymes suggests their differential functions in wheat seeds...
  52. ncbi request reprint Crystal structure of neoculin: insights into its sweetness and taste-modifying activity
    Akiko Shimizu-Ibuka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Japan
    J Mol Biol 359:148-58. 2006
    ....
  53. pmc Luteolin and quercetin affect the cholesterol absorption mediated by epithelial cholesterol transporter niemann-pick c1-like 1 in caco-2 cells and rats
    Mari Nekohashi
    Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
    PLoS ONE 9:e97901. 2014
    ..These results suggest that luteolin and quercetin reduce high blood cholesterol levels by specifically inhibiting intestinal cholesterol absorption mediated by NPC1L1. ..