Tomohisa Kuzuyama

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. pmc An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190
    K Kaneda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 98:932-7. 2001
  2. ncbi request reprint Diversity of the biosynthesis of the isoprene units
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nat Prod Rep 20:171-83. 2003
  3. ncbi request reprint Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 66:1619-27. 2002
  4. ncbi request reprint Detection of the mevalonate pathway in Streptomyces species using the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    J Antibiot (Tokyo) 55:919-23. 2002
  5. doi request reprint Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus
    Takuya Ouchi
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    Nat Chem Biol 9:277-83. 2013
  6. pmc Mechanism of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus
    Takuya Okada
    Biotechnology Research Center, University of Tokyo, Tokyo 113 8657, Japan
    J Biol Chem 285:4195-205. 2010
  7. doi request reprint Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus
    Akira Horie
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Nat Chem Biol 5:673-9. 2009
  8. doi request reprint Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase from Thermus thermophilus
    Ayako Yoshida
    Biotechnology Research Center, The University of Tokyo, Japan
    FEBS J 276:3124-36. 2009
  9. doi request reprint Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution
    Yumewo Suzuki
    Biotechnology Research Center, University of Tokyo, Bunkyo ku, Japan
    Biochem J 431:401-10. 2010
  10. pmc Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway
    Eiji Okamura
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 107:11265-70. 2010

Collaborators

Detail Information

Publications62

  1. pmc An unusual isopentenyl diphosphate isomerase found in the mevalonate pathway gene cluster from Streptomyces sp. strain CL190
    K Kaneda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 98:932-7. 2001
    ..aureus and of the different enzymatic properties of mammalian (type 1) and S. aureus (type 2) isomerases, this unusual enzyme is considered to be a suitable molecular target for the screening of antibacterial drugs specific to S. aureus...
  2. ncbi request reprint Diversity of the biosynthesis of the isoprene units
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    Nat Prod Rep 20:171-83. 2003
    ..e type 2 IPP isomerase. The biosynthesis of terpenoids produced by actinomycetes is also reviewed. 117 references are cited...
  3. ncbi request reprint Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biosci Biotechnol Biochem 66:1619-27. 2002
    ..chem.qmw.ac.uk/iubmb/enzyme/reaction/terp/nonMVA.html...
  4. ncbi request reprint Detection of the mevalonate pathway in Streptomyces species using the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo 113 0032, Japan
    J Antibiot (Tokyo) 55:919-23. 2002
  5. doi request reprint Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus
    Takuya Ouchi
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    Nat Chem Biol 9:277-83. 2013
    ..Phylogenetic analysis reveals that gene duplication events at different stages of evolution led to ArgX and LysX...
  6. pmc Mechanism of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus
    Takuya Okada
    Biotechnology Research Center, University of Tokyo, Tokyo 113 8657, Japan
    J Biol Chem 285:4195-205. 2010
    ..Replacement of His-72 by leucine made HCS resistant to lysine inhibition, demonstrating the regulatory role of this conserved residue...
  7. doi request reprint Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus
    Akira Horie
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Nat Chem Biol 5:673-9. 2009
    ..This study thus reveals the previously unknown function of a small protein in primary metabolism...
  8. doi request reprint Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase from Thermus thermophilus
    Ayako Yoshida
    Biotechnology Research Center, The University of Tokyo, Japan
    FEBS J 276:3124-36. 2009
    ..Comparison of the crystal structures of TtAKbeta and the regulatory subunit of C. glutamicum AK showed that the well-packed hydrophobic core and high Pro content in loops contribute to the high thermostability of TtAKbeta...
  9. doi request reprint Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution
    Yumewo Suzuki
    Biotechnology Research Center, University of Tokyo, Bunkyo ku, Japan
    Biochem J 431:401-10. 2010
    ..On the basis of the crystal structure, possible reasons for enhancement of the turnover number are discussed...
  10. pmc Unprecedented acetoacetyl-coenzyme A synthesizing enzyme of the thiolase superfamily involved in the mevalonate pathway
    Eiji Okamura
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Proc Natl Acad Sci U S A 107:11265-70. 2010
    ..This result suggests that nphT7 can be used to significantly increase the concentration of acetoacetyl-CoA in cells, eventually leading to the production of useful terpenoids and poly-beta-hydroxybutyrate...
  11. ncbi request reprint Structural Insight into concerted inhibition of alpha 2 beta 2-type aspartate kinase from Corynebacterium glutamicum
    Ayako Yoshida
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Mol Biol 368:521-36. 2007
    ..glutamicum. A putative Lys-binding site and the inhibitory mechanism of CgAK are discussed...
  12. pmc Functional characterization of the promiscuous prenyltransferase responsible for furaquinocin biosynthesis: identification of a physiological polyketide substrate and its prenylated reaction products
    Takuto Kumano
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 285:39663-71. 2010
    ..The promiscuous substrate specificity of Fur7 was demonstrated with respect to prenyl acceptor substrates and prenyl donor substrates. The steady-state kinetic constants of Fur7 with each prenyl acceptor substrate were also calculated...
  13. doi request reprint Mechanism for multiple-substrates recognition of alpha-aminoadipate aminotransferase from Thermus thermophilus
    Takeo Tomita
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Proteins 75:348-59. 2009
    ..The crystal structures and site-directed mutagenesis revealed that intersubunit-electrostatic interactions contribute to the elevated thermostability of this enzyme...
  14. ncbi request reprint NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids
    Taro Ozaki
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Antibiot (Tokyo) 62:385-92. 2009
    ..This study shows that NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids...
  15. pmc Mechanism of concerted inhibition of alpha2beta2-type hetero-oligomeric aspartate kinase from Corynebacterium glutamicum
    Ayako Yoshida
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 285:27477-86. 2010
    ..This study shows not only the first crystal structures of alpha(2)beta(2)-type AK but also the mechanism of concerted inhibition in CgAK...
  16. pmc Novel tryptophan metabolism by a potential gene cluster that is widely distributed among actinomycetes
    Taro Ozaki
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 288:9946-56. 2013
    ..Finally, dehydration of 5-DMAIAOx presumably occurs to yield 5-DMAIAN. This study provides insight into the biosynthesis of prenylated indoles that have been purified from actinomycetes...
  17. ncbi request reprint Alteration of coenzyme specificity of lactate dehydrogenase from Thermus thermophilus by introducing the loop region of NADP(H)-dependent malate dehydrogenase
    Takeo Tomita
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 70:2230-5. 2006
    ..The effect of the replacements on coenzyme specificity is discussed based on in silico simulation of the three-dimensional structure of the lactate dehydrogenase mutant...
  18. doi request reprint The herbicide ketoclomazone inhibits 1-deoxy-D-xylulose 5-phosphate synthase in the 2-C-methyl-D-erythritol 4-phosphate pathway and shows antibacterial activity against Haemophilus influenzae
    Yukiko Matsue
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    J Antibiot (Tokyo) 63:583-8. 2010
    ..These data reveal the inhibitory mechanism of ketoclomazone on DXP synthase...
  19. pmc Two ATP-binding cassette transporters involved in (S)-2-aminoethyl-cysteine uptake in thermus thermophilus
    Yuko Kanemaru
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    J Bacteriol 195:3845-53. 2013
    ..The structures revealed that TTC0807 recognizes various basic amino acids by changing the side-chain conformation of Glu19, which interacts with the side-chain amino groups of the substrates. ..
  20. pmc Chemoenzymatic syntheses of prenylated aromatic small molecules using Streptomyces prenyltransferases with relaxed substrate specificities
    Takuto Kumano
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Bioorg Med Chem 16:8117-26. 2008
    ..Kinetic studies with NphB reveal that the prenylation reaction follows a sequential ordered mechanism...
  21. ncbi request reprint Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus
    Kei Fujiwara
    Biotechnology Research Center, University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Microbiology 152:3585-94. 2006
    ..Furthermore, an alpha-galactosidase reporter assay for this promoter indicated that arginine repressed the promoter in an argR-dependent manner. These results indicate that lysine biosynthesis is regulated by arginine in T. thermophilus...
  22. pmc Structural basis for leucine-induced allosteric activation of glutamate dehydrogenase
    Takeo Tomita
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 286:37406-13. 2011
    ..thermophilus GDH insensitive to leucine. Equivalent amino acid replacement caused a similar loss of sensitivity to leucine in human GDH2, suggesting that human GDH2 also uses the same allosteric site for regulation by leucine...
  23. ncbi request reprint Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase mutant
    Takeo Tomita
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 347:502-8. 2006
    ....
  24. doi request reprint Hetero-oligomeric glutamate dehydrogenase from Thermus thermophilus
    Takeo Tomita
    Biotechnology Research Center, Department of Biotechnology, The University of Tokyo, Tokyo 113 8657, Japan
    Microbiology 156:3801-13. 2010
    ..This study provides the first finding, to our knowledge, of a hetero-oligomeric GDH that can be regulated allosterically...
  25. doi request reprint Dual roles of a conserved pair, Arg23 and Ser20, in recognition of multiple substrates in alpha-aminoadipate aminotransferase from Thermus thermophilus
    Takuya Ouchi
    Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo ku, Japan
    Biochem Biophys Res Commun 388:21-7. 2009
    ....
  26. doi request reprint The single cellular green microalga Botryococcus braunii, race B possesses three distinct 1-deoxy-D-xylulose 5-phosphate synthases
    Daisuke Matsushima
    Department of Aquatic Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo, Tokyo 113 8657, Japan
    Plant Sci 185:309-20. 2012
    ..This difference to other microalgae seems to mirror its special needs for extensive triterpene production by increasing the metabolic flow through the MEP pathway...
  27. ncbi request reprint Bacillus subtilis ypgA gene is fni, a nonessential gene encoding type 2 isopentenyl diphosphate isomerase
    Motoki Takagi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Biosci Biotechnol Biochem 68:132-7. 2004
    ..Disruption of the ypgA3 gene was not lethal to B. subtilis. These results indicate that Bacillus ypgA3 gene is fni, a nonessential gene encoding type 2 IPP isomerase...
  28. pmc Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton
    Ayuko Meguro
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Chembiochem 14:316-21. 2013
    ..SANK 60404. DtcycA and DtcycB are novel bacterial diterpene cyclases for the synthesis of the cembrane skeleton...
  29. doi request reprint New glycosylated derivatives of versipelostatin, the GRP78/Bip molecular chaperone down-regulator, from Streptomyces versipellis 4083-SVS6
    Ping Zhao
    Laboratory of Cell Biotechnology, Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo113 8657, Japan
    Org Biomol Chem 7:1454-60. 2009
    ..5 and 4.3 microM. These results suggest that the alpha-L-oleandropyranosyl (1-->4)-beta-D-digitoxopyranosyl residue in the sugar moiety may play an important role in down-regulating GRP78 expression induced by 2-deoxyglucose...
  30. ncbi request reprint Cloning and heterologous expression of the cyclooctatin biosynthetic gene cluster afford a diterpene cyclase and two p450 hydroxylases
    Seung Young Kim
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Chem Biol 16:736-43. 2009
    ....
  31. pmc Crystal structure of tetrameric homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus: involvement of hydrophobic dimer-dimer interaction in extremely high thermotolerance
    Junichi Miyazaki
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Bacteriol 187:6779-88. 2005
    ..Both the crystal structure and the mutation analysis indicate that tetramer formation is involved in the extremely high thermotolerance of TtHICDH...
  32. ncbi request reprint Identification of 8-amino-2,5,7-trihydroxynaphthalene-1,4-dione, a novel intermediate in the biosynthesis of Streptomyces meroterpenoids
    Shota Isogai
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Bioorg Med Chem Lett 22:5823-6. 2012
    ..We propose that the biosynthetic pathway generating 8-amino-flaviolin is the common route for the biosynthesis of Streptomyces meroterpenoids...
  33. pmc Two distinct pathways for essential metabolic precursors for isoprenoid biosynthesis
    Tomohisa Kuzuyama
    Biotechnology Research Center, The University of Tokyo, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 88:41-52. 2012
    ..This review article focuses on our significant contributions to a comprehensive understanding of the biosynthesis of IPP and DMAPP...
  34. pmc Purification, crystallization and preliminary X-ray analysis of the regulatory subunit of aspartate kinase from Thermus thermophilus
    Ayako Yoshida
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:96-8. 2007
    ..Diffraction data were collected to 2.15 A at a synchrotron source. The crystal belongs to the cubic space group P4(3)32 or P4(1)32, with unit-cell parameters a = b = c = 141.8 A...
  35. ncbi request reprint Nucleotide sequences of genes encoding allosamidin-sensitive and -insensitive chitinases produced by allosamidin-producing Streptomyces
    Hiroshi Matsuura
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
    Biosci Biotechnol Biochem 67:2002-5. 2003
    ..AJ9463. In this study, we cloned and sequenced the genes encoding the two chitinases, which clarified that chitinase S and IS belong to the family 18 and 19 chitinase, respectively...
  36. ncbi request reprint Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice
    Atsushi Okada
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Tokyo, Japan
    Plant Mol Biol 65:177-87. 2007
    ..These results suggest that activation of the MEP pathway is required to supply sufficient terpenoid precursors for the production of phytoalexins in infected rice plants...
  37. ncbi request reprint [Overlooked targets for the development of antibacterial and antimalarial drugs and herbicides: diverse biosynthetic systems for the isoprene units]
    Motoki Takagi
    Tanpakushitsu Kakusan Koso 47:58-65. 2002
  38. ncbi request reprint [Biosynthesis of a hybrid terpenoid polyketide-derived natural product and production of useful prenylated compounds by using prenyltransferase]
    Tomohisa Kuzuyama
    Tanpakushitsu Kakusan Koso 52:1736-41. 2007
  39. ncbi request reprint Cyclolavandulyl skeleton biosynthesis via both condensation and cyclization catalyzed by an unprecedented member of the cis-isoprenyl diphosphate synthase superfamily
    Taro Ozaki
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Am Chem Soc 136:4837-40. 2014
    ..This enzyme is an unprecedented terpene synthase that catalyzes both the condensation of the C5 isoprene units and subsequent cyclization to form the cyclolavandulyl monoterpene structure. ..
  40. ncbi request reprint Heterologous mevalonate production in Streptomyces lividans TK23
    Tomohisa Kuzuyama
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan
    Biosci Biotechnol Biochem 68:931-4. 2004
    ..strain CL190...
  41. doi request reprint Identification and biosynthesis of new acyloins from the thermophilic bacterium Thermosporothrix hazakensis SK20-1(T)
    Jin Soo Park
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657 Japan
    Chembiochem 15:527-32. 2014
    ..Through extensive analysis of the Thzk0150-catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4-methyl-2-oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor. ..
  42. ncbi request reprint Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)
    Takeo Tomita
    Biotechnology Research Center, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 334:613-8. 2005
    ..This has provided the first evidence for the alternative binding mode of the nicotinamide coenzyme, that has pseudo-symmetry in its structure, in a single enzyme...
  43. ncbi request reprint Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus
    Taishi Tsubouchi
    Biotechnology Research Center, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 280:18511-6. 2005
    ..This is the first report demonstrating the transcriptional regulation of amino acid biosynthesis in T. thermophilus...
  44. ncbi request reprint A relationship between the mevalonate pathway and isoprenoid production in actinomycetes
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Antibiot (Tokyo) 56:957-66. 2003
    ..This result suggested that the presence of the mevalonate pathway might be a good landmark to detect the production of isoprenoid compounds by actinomycetes...
  45. ncbi request reprint Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of gibberellins in Arabidopsis
    Hiroyuki Kasahara
    Laboratory for Cellular Growth and Development, Growth Physiology Research Group, Plant Science Center, RIKEN The Institute of Physical and Chemical Research, Hirosawa 2 1, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:45188-94. 2002
    ..We also provide evidence to suggest that the MVA pathway still contributes to GA biosynthesis when this pathway is limiting...
  46. ncbi request reprint Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin
    Yoshimitsu Hamano
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Biol Chem 277:37098-104. 2002
    ..These results suggested that an actual reaction catalyzed by the ORF12 was the conversion of TDP into TTE in vivo...
  47. ncbi request reprint Growth-phase dependent expression of the mevalonate pathway in a terpenoid antibiotic-producing Streptomyces strain
    Yoshimitsu Hamano
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Biosci Biotechnol Biochem 66:808-19. 2002
    ....
  48. ncbi request reprint Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis
    Kazunori Okada
    Department of Biology, Tokyo Gakugei University, Nukuikitamachi 4 1 1, Koganei Shi, Tokyo, 184 8501, Japan
    Planta 215:339-44. 2002
    ..These observations suggest that both AtMECT and DXR are important in the synthesis of isopentenyl diphosphate and dimethylallyl diphosphate and that ent-kaurene is mainly produced through the nonmevalonate pathway in the plastid...
  49. ncbi request reprint Crystal structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase complexed with cofactors: implications of a flexible loop movement upon substrate binding
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Biochem 131:313-7. 2002
    ..A flexible loop covering the substrate binding site plays an important role in the enzymatic reaction and the determination of substrate specificity...
  50. ncbi request reprint Preferential recognition of a microbial metabolite by human Vgamma2Vdelta2 T cells
    Kia Joo Puan
    Division of Rheumatology, Department of Internal Medicine and the Interdisciplinary Graduate Program in Immunology, University of Iowa College of Medicine, EMRB 400F, Iowa City, IA 52242, USA
    Int Immunol 19:657-73. 2007
    ..This preferential stimulation by a common microbial isoprenoid metabolite allows Vgamma2Vdelta2 T cells to respond to a broad array of pathogens using this pathway...
  51. ncbi request reprint Mechanistic studies of HPP epoxidase: configuration of the substrate governs its enzymatic fate
    Zongbao Zhao
    Division of Medicinal Chemistry, College of Pharmacy, Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
    Angew Chem Int Ed Engl 41:4529-32. 2002
  52. ncbi request reprint Structure and catalytic mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis
    Hiroyuki Kishida
    Protein Design Laboratory, Graduate School of Integrated Science, Yokohama City University, 1 7 29 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    Acta Crystallogr D Biol Crystallogr 59:23-31. 2003
    ..At the high resolution of the refinement (1.6 A) the positions and coordination of the magnesium ions in the active site are clearly seen...
  53. ncbi request reprint Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398
    J Biochem 133:83-91. 2003
    ..These results showed that the amino acid residue at the 57th (58th) position after the FARM also played an important role in determination of the product specificity...
  54. ncbi request reprint Cloning and characterization of 2-C-methyl-D: -erythritol 2,4-cyclodiphosphate synthase (MECS) gene from Ginkgo biloba
    Sang Min Kim
    Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, Seoul, 151 921, Korea
    Plant Cell Rep 25:829-35. 2006
    ....
  55. ncbi request reprint fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis
    Kia Joo Puan
    Division of Rheumatology, Department of Internal Medicine and the Interdisciplinary Group in Immunology, University of Iowa, EMRB 340F, Iowa City, IA 52242, USA
    FEBS Lett 579:3802-6. 2005
    ..Thus, the essential role for flavodoxin I in E. coli is in the MEP pathway for isoprenoid biosynthesis...
  56. pmc Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products
    Tomohisa Kuzuyama
    Jack Skirball Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 435:983-7. 2005
    ..These crystal structures, coupled with in vitro assays, provide a basis for understanding and potentially manipulating the regio-specific prenylation of aromatic small molecules using this structurally unique family of aromatic PTases...
  57. ncbi request reprint Presence of copalyl diphosphate synthase gene in an actinomycete possessing the mevalonate pathway
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Antibiot (Tokyo) 57:739-47. 2004
    ..These results again showed that actinomycetes possessing the mevalonate pathway usually produce an isoprenoid and that its biosynthetic gene cluster exists in adjacent the mevalonate pathway gene cluster...
  58. pmc Biosynthesis of a natural polyketide-isoprenoid hybrid compound, furaquinocin A: identification and heterologous expression of the gene cluster
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Bacteriol 188:1236-44. 2006
    ....
  59. ncbi request reprint Cloning and functional characterization of 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (GbMECT) gene from Ginkgo biloba
    Sang Min Kim
    Program in Applied Life Chemistry, School of Agricultural Biotechnology, Seoul National University, Sinlim Dong, Gwanak Gu, Seoul 151 921, Republic of Korea Plant Metabolism Research Center, Kyung Hee University, Yongin 449 701, Republic of Korea
    Phytochemistry 67:1435-41. 2006
    ..Full 88 N-terminal residues were necessary to deliver the protein into the chloroplast as shown by protein-targeting analysis with GFP as a reporter protein in Arabidopsis thaliana protoplasts...
  60. ncbi request reprint Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis
    Takashi Wada
    Genomic Sciences Center, RIKEN Yokohama Institute, and Protein Design Laboratory, Yokohama City University, 1 7 29 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    J Biol Chem 278:30022-7. 2003
    ..Assaying the enzyme showed that CDP-ME kinase will phosphorylate CDP-ME but not 4-(uridine 5'-diphospho)-2-C-methyl-D-erythritol, indicating the substrate pyrimidine moiety is involved in important interactions with the enzyme...
  61. pmc Structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a quaternary complex with a magnesium ion, NADPH and the antimalarial drug fosmidomycin
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:466-70. 2007
    ..On the other hand, no electron density was observed for the nicotinamide-ribose portion of NADPH and the position of Asp149 anchoring Mg(2+) was shifted by NADPH in the active site...
  62. ncbi request reprint Identification of class 2 1-deoxy-D-xylulose 5-phosphate synthase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase genes from Ginkgo biloba and their transcription in embryo culture with respect to ginkgolide biosynthesis
    Sang Min Kim
    School of Agricultural Biotechnology, Seoul National University, Korea
    Planta Med 72:234-40. 2006
    ..Exclusive transcription of ginkgolide biosynthesis-specific LPS and GbDXS2 in roots and the appearance of ginkgolides in leaves was consistent with translocation of the compounds from roots to leaves...