Research Topics
| Hidekazu KuwayamaSummaryAffiliation: University of Tsukuba Country: Japan Publications
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Detail Information
Publications
Arachidonic acid enhances caffeine-induced cell death via caspase-independent cell deathHidekazu Kuwayama
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Tennodai 1 1 1, Ibaraki 305 8572, Japan
Sci Rep 2:577. 2012..Our results indicate that arachidonic acid may be a general second messenger that negatively regulates caffeine tolerance via a caspase-independent cell death cascade, which leads to multiple effects in eukaryotic cells...
DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies: Evidence from gene targeting of Dictyostelium discoideumHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Tennodai 1 1 1, Ibaraki 305 8572, Japan
J Biotechnol 133:418-23. 2008..Thus, the simplicity and versatility of this method applicable to any type of cell may increase the application of gene targeting...- Desalted deep sea water increases transformation and homologous recombination efficiencies in Dictyostelium discoideumHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
J Mol Microbiol Biotechnol 14:157-62. 2008..These results indicate that a simple modification of the solvent medium remarkably enhanced the isolation of transformants and gene-targeted clones, which had previously been difficult to isolate... - A stress response kinase, KrsA, controls cAMP relay during the early development of Dictyostelium discoideumTetsuya Muramoto
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennoudai, Tsukuba, Ibaraki 305 8572, Japan
Dev Biol 305:77-89. 2007..These results strongly suggest that krsA is involved in cAMP relay, and that signaling pathways for multicellular development have evolved in unison with the stress response... - Artificial compounds differentially control Dictyostelium chemotaxis and cell differentiationHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305 8572, Japan
Cell Struct Funct 36:21-6. 2011..Furthermore, our results indicate that DIF derivatives can be utilized to analyze the DIF-signaling pathways... - A putative serpentine receptor gene tasA required for normal morphogenesis of primary stalk and branch structure in Polysphondylium pallidumYoshinori Kawabe
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
Gene 285:291-9. 2002.... - PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectorsHidekazu Kuwayama
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Tennodai 1-1-1, Ibaraki 305-8572, Japan
Nucleic Acids Res 30:E2. 2002..This method is simple, rapid and relatively inexpensive. In addition, there is the freedom of attaching long flanking regions to any selectable marker cassette... - Regulation of ammonia homeostasis by the ammonium transporter AmtA in Dictyostelium discoideumRyuji Yoshino
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan 305 8572
Eukaryot Cell 6:2419-28. 2007..Taken together, our data clearly demonstrate that AmtA regulates ammonia homeostasis and plays important roles in multiple developmental processes in Dictyostelium... - Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxisHidekazu Kuwayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
PLoS ONE 4:e6658. 2009.... 
Temporal and non-permanent division of labor during sorocarp formation in the social amoeba Acytostelium subglobosumKurato Mohri
Faculty of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8572, Japan
Dev Biol 375:202-9. 2013..Instead of the permanent division of labor observed in D. discoideum, A. subglobosum produces fruiting bodies by all cells contributing to the formation of the stalk as well as forming spores...- GMF is an evolutionarily developed Adf/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeletonKentaro Nakano
Department of Structural Biosciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba, Ibaraki, Japan
Cytoskeleton (Hoboken) 67:373-82. 2010..Given the functional plasticity of ADF-H, GMF-family proteins possibly have changed their target from conventional actin to Arps through molecular evolution... - A transcriptional profile of multicellular development in Dictyostelium discoideumNancy Van Driessche
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX 77030, USA
Development 129:1543-52. 2002..Our findings provide new perspectives on the complexity of the developmental program and the fraction of the genome that is regulated during development... - MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium sppSatomi Matsuoka
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
Eukaryot Cell 2:638-45. 2003..Hence, we propose that this enzyme plays an irreplaceable role in the survival strategy of Dictyostelium cells to form spores for their efficient dispersal in nature... - Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during developmentHaruyo Sakamoto
Department of Biology, Graduate School of Science, Osaka University, Machikaneyama cho 1 16, Toyonaka, Osaka 560 0043, Japan
Dev Growth Differ 45:507-14. 2003..Such distinct spatial expression patterns suggest that the CBP might be involved in morphogenesis and might have their own roles either in prespore or in prestalk cell differentiation of Dictyostelium... - Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in DictyosteliumSatomi Matsuoka
Department of Biology, Graduate School of Science, Osaka University, 1-16 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan
Dev Growth Differ 46:195-9. 2004..Thus, most of the developmental consequences of excess cyclopropane fatty acids appear to result from impaired cAMP relay... - An orderly retreat: Dedifferentiation is a regulated processMariko Katoh
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 101:7005-10. 2004..These observations establish dedifferentiation as a genetically determined process and suggest the existence of a developmental checkpoint that ensures a return path to the undifferentiated state... - Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing proteinSatomi Matsuoka
Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan
J Cell Sci 119:1071-9. 2006..These dynamic properties might be a common feature of signaling components involved in chemotaxis... - Periodic signaling controlled by an oscillatory circuit that includes protein kinases ERK2 and PKAMineko Maeda
Department of Biology, Graduate School of Science, Osaka University, Machikaneyama cho 1 16, Toyonaka, Osaka 560 0043, Japan
Science 304:875-8. 2004..Similar oscillatory processes may occur in cells of many different species...