Research Topics
Genomes and GenesSpecies | Hiroaki KawasakiSummaryAffiliation: University of Tokyo Country: Japan Publications
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Detail Information
Publications
Induction of DNA methylation and gene silencing by short interfering RNAs in human cellsHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
Nature 431:211-7. 2004....- Transcriptional gene silencing by short interfering RNAsHiroaki Kawasaki
The University of Tokyo, Department of Chemistry and Biotechnology, School of Engineering, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
Curr Opin Mol Ther 7:125-31. 2005..Recently, siRNAs were demonstrated to induce transcriptional gene silencing (TGS) via DNA methylation in human cells. This review will focus on TGS by siRNAs, such as DNA methylation and histone methylation... - Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cellsHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
Nature 423:838-42. 2003..Thus, our results indicate that miR-23 regulates the expression of Hes1 at the post-transcriptional level, and participates in retinoic-acid-induced neuronal differentiation of NT2 cells... - Identification of metastasis-related genes in a mouse model using a library of randomized ribozymesEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
J Biol Chem 279:38083-6. 2004..The further characterization of these candidate genes would provide clues to the complex mechanism(s) of metastasis... - Maxizyme technologyMayu Iyo
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
Methods Mol Biol 252:257-65. 2004..Thus, maxizyme technology is not limited to one specific case, but may have broad general applicability in molecular biology and in molecular gene therapy... - Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivoKeisuke Oshima
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan
Cancer Res 63:6809-14. 2003..Our findings confirm the potential utility of maxizymes and shRNAs as therapeutic agents... - Cell migration and metastasis as targets of small RNA-based molecular genetic analysesEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan
J Muscle Res Cell Motil 25:303-8. 2004..We then describe our promising novel approach that uses randomized ribozyme libraries for identification of genes involved in cell migration, a consistent feature of metastatic cells... - LIM kinase-2 targeting as a possible anti-metastasis therapyEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan
J Gene Med 6:357-63. 2004..CONCLUSIONS: Specific targeting of metastatic and malignant properties of tumor cells by LIMK-2 ribozyme may serve as an effective therapy for invasive tumors with minimum effect on the surrounding normal cells... - World of small RNAs: from ribozymes to siRNA and miRNAHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Differentiation 72:58-64. 2004..This article reviews our contributions to the understanding of structure, the function of small RNAs, their use in biotechnology, and the understanding of phenotypes such as apoptosis, metastasis, and differentiation... - Identification of genes responsible for cell migration by a library of randomized ribozymesEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan
Cancer Res 63:119-24. 2003..Ribozymes identified in this study, including the ribozymes against ROCK1, might be useful in understanding the mechanisms of cell migration and metastasis... - Functional gene discovery using hybrid ribozyme librariesYoshio Kato
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
Methods Mol Biol 252:245-56. 2004..This chapter describes a method known as the gene-discovery system to identify novel functional genes related to a specific phenotype. Our gene-discovery system should be a powerful tool for post-genome research... - siRNA induced transcriptional gene silencing in mammalian cellsHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan
Cell Cycle 4:442-8. 2005..e., directed control of a genes expression) and biologically in understanding the evolution of the cell... - Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymesEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, Hongo, Tokyo 113-8656, Japan
Proc Natl Acad Sci U S A 100:5616-21. 2003..Our system for gene identification by using ribozymes and the functional analysis of target genes should help to clarify the complex mechanisms of invasion and metastasis and might provide information that is relevant to cancer therapy... - Identification of a caspase 3-independent role of pro-apoptotic factor Bak in TNF-alpha-induced apoptosisEigo Suyama
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Japan
FEBS Lett 528:63-9. 2002..This study demonstrates the caspase 3-independent function of Bak in the TNF-alpha-induced apoptotic pathway... - siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cellsHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 8656, Japan
Nucleic Acids Res 31:981-7. 2003..However, it is important to avoid homologous sequences within a target mRNA in a given protein family. Our diced siRNA system should be a powerful tool for the inactivation of genes in mammalian cells... - Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cellsHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Bunkyou ku, Tokyo 113 8656, Japan
Nucleic Acids Res 31:700-7. 2003..Our tRNA-dsRNA technology should be a powerful tool for studies of the mechanism of RNAi and the functions of various genes in mammalian cells with potential utility as a therapeutic agent... - [RNAi-mediated specific gene silencing and its application to medical treatments of viral infectious disease]Hiroshi Minoshima
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Uirusu 53:7-14. 2003 - Construction of microRNA-containing vectors for expression in mammalian cellsYoko Fukuda
Department of Chemistry and Biotechnology, The University of Tokyo, Japan
Methods Mol Biol 338:167-73. 2006..miRNA expression vectors containing human RNA polymerase II or III promoters, and utilizing a flexible vector system, can be useful for functional analysis... - Helicase-attached novel hybrid ribozymesHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
Methods Mol Biol 252:237-43. 2004..Thus, hybrid ribozymes can catalyze cleavage at the specified target site within an RNA in vivo almost independently of the secondary or tertiary structure of the target RNA around the cleavage site... - Enhancement in the cleavage activity of a hammerhead ribozyme by cationic comb-type polymers and an RNA helicase in vitroHideo Akashi
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan
J Biochem (Tokyo) 131:687-92. 2002..These approaches should be useful in the development of efficient gene-inactivating reagents in the post-genomic era... - Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicaseHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan
EMBO Rep 3:443-50. 2002..This application of a randomized library of hybrid ribozymes represents a simple, powerful method for the identification of genes associated with specific phenotypes in the post-genome era... - Allosterically controllable maxizymes for molecular gene therapyMayu Iyo
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Japan
Curr Opin Mol Ther 4:154-65. 2002..Thus, maxizyme technology is not limited to a specific case but may have broad general applicability in molecular biology and in molecular gene therapy... - A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme librariesHiroaki Kawasaki
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
Nucleic Acids Res 30:3609-14. 2002.... - Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme librariesHiroaki Kawasaki
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan
Nat Biotechnol 20:376-80. 2002..We identified many pro-apoptotic genes and partial sequences of previously uncharacterized genes using this method. Our gene discovery system should be generally applicable to the identification of functional genes in various systems... - Lysophosphatidic acid acyltransferase-beta is a prognostic marker and therapeutic target in gynecologic malignanciesGregory M Springett
Memorial Sloan-Kettering Cancer Center, Developmental Chemotherapy Laboratory, Department of Medicine, New York, New York [corrected] USA
Cancer Res 65:9415-25. 2005..These findings identify LPAAT-beta as a potential prognostic and therapeutic target in ovarian and endometrial cancer... - Characterization of a hemolytic protein, identified as histone H4, from the skin of the Japanese tree frog Hyla japonica (Hylidae)Hiroaki Kawasaki
Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274 8510, Japan
Comp Biochem Physiol B Biochem Mol Biol 149:120-5. 2008..japonica histone H4 gene, which acts as a transcription termination signal, contained a nucleotide sequence (5'-GGCTCTCCTCAGAGCC-3') with unusual structural features not seen in other histone genes... - Overexpression and ribozyme-mediated targeting of transcriptional coactivators CREB-binding protein and p300 revealed their indispensable roles in adipocyte differentiation through the regulation of peroxisome proliferator-activated receptor gammaNobuyuki Takahashi
Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
J Biol Chem 277:16906-12. 2002..These data suggest that both CBP and p300 are indispensable for the full activation of PPARgamma and adipocyte differentiation and that CBP and p300 do not mutually complement in the process... - Analysis of a mitochondrial apoptotic pathway using Bid-targeted ribozymes in human MCF7 cells in the absence of a caspase-3-dependent pathwayReiko Onuki
Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology AIST, Central 4, Higashi, Tsukuba Science City 305 8562, Japan
Antisense Nucleic Acid Drug Dev 13:75-82. 2003..Therefore, it became clear for the first time that caspase 9 by itself can activate caspase 7 in the absence of the caspase 3-dependent pathway in TNF-alpha-induced apoptosis by the use of specific ribozymes... - Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymesRenu Wadhwa
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan
EMBO Rep 4:595-601. 2003.... - An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's diseaseReiko Onuki
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Higashi, Tsukuba Science City, Japan
EMBO J 23:959-68. 2004..We also detected the accumulation of phosphorylated PKR in the nuclei of autopsied brain tissues in Alzheimer's disease. Thus, PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease... - Antimicrobial peptides from the skin of the Japanese mountain brown frog Rana ornativentris: evidence for polymorphism among preprotemporin mRNAsAya Ohnuma
Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
Peptides 28:524-32. 2007.... - Down-regulation of endogenous Wt1 expression by Sry transgene in the murine embryonic mesonephros-derived M15 cell lineMasanori Ito
Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
J Reprod Dev 52:415-27. 2006..e., turning on Sry expression on one hand, and being down-regulated by its product, Sry, on the other, possibly forming a type of negative feed-back mechanism. Further work is needed to substantiate this view... - Developmental and triiodothyronine-induced expression of genes encoding preprotemporins in the skin of Tago's brown frog Rana tagoiAya Ohnuma
Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan
Gen Comp Endocrinol 146:242-50. 2006.... - Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activationReiko Onuki
Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Central 4, 1-1-1 Higashi, Tsukuba Science City 305-8562, Japan
Proc Natl Acad Sci U S A 99:14716-21. 2002.... - [Development of siRNA libraries by in vitro dicing and optimized efficient expression vectors for siRNAs in mammalian cells]Hiroaki Kawasaki
Tanpakushitsu Kakusan Koso 48:1638-45. 2003 - A protein with antimicrobial activity in the skin of Schlegel's green tree frog Rhacophorus schlegelii (Rhacophoridae) identified as histone H2BHiroaki Kawasaki
Department of Biology, Faculty of Science, Toho University, Funabashi, 274-8510, Chiba, Japan
Biochem Biophys Res Commun 312:1082-6. 2003..The data provide further evidence that histones play a role in the defense against microorganisms... - Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)Shinji Fukuda
Division of Genome Analysis, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan
J Biochem (Tokyo) 131:349-57. 2002..The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells...