Hiroaki Kawasaki

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi LIM kinase-2 targeting as a possible anti-metastasis therapy
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    J Gene Med 6:357-63. 2004
  2. ncbi Induction of DNA methylation and gene silencing by short interfering RNAs in human cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nature 431:211-7. 2004
  3. ncbi Transcriptional gene silencing by short interfering RNAs
    Hiroaki Kawasaki
    The University of Tokyo, Department of Chemistry and Biotechnology, School of Engineering, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Curr Opin Mol Ther 7:125-31. 2005
  4. ncbi Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nature 423:838-42. 2003
  5. ncbi Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 279:38083-6. 2004
  6. ncbi Maxizyme technology
    Mayu Iyo
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
    Methods Mol Biol 252:257-65. 2004
  7. ncbi Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo
    Keisuke Oshima
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113 8656, Japan
    Cancer Res 63:6809-14. 2003
  8. ncbi Cell migration and metastasis as targets of small RNA-based molecular genetic analyses
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan
    J Muscle Res Cell Motil 25:303-8. 2004
  9. ncbi World of small RNAs: from ribozymes to siRNA and miRNA
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Differentiation 72:58-64. 2004
  10. ncbi Identification of genes responsible for cell migration by a library of randomized ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    Cancer Res 63:119-24. 2003

Collaborators

  • Renu Wadhwa
  • J M Conlon
  • Sunil C Kaul
  • Tatsuhiko Kasaoka
  • Toshihiro Akaike
  • Kevin V Morris
  • Yoshio Kato
  • Masaki Warashina
  • Akira Nagasaki
  • Y Bando
  • Yasutomi Kamei
  • Naohito Aoki
  • Kazunari Taira
  • Eigo Suyama
  • Reiko Onuki
  • Aya Ohnuma
  • Tadashi Baba
  • Mayu Iyo
  • Shawichi Iwamuro
  • Yoko Fukuda
  • Masanori Ito
  • Makoto Miyagishi
  • Gregory M Springett
  • Motowo Nakajima
  • Keisuke Oshima
  • Hiroshi Minoshima
  • Nobuyuki Takahashi
  • Hideo Akashi
  • Shinji Fukuda
  • Thierry Jouenne
  • Jerome Leprince
  • Hubert Vaudry
  • Laurent Coquet
  • Keiko Yamaguchi
  • Chisato Murata
  • Chikashi Tachi
  • Stefano Di Giovine
  • Gabriella Pezzoni
  • Robert Soslow
  • Dipika Misra
  • Lynn Bonham
  • Chia Ma
  • Amanda Hummer
  • Jakob Dupont
  • Jack Singer
  • Irina Linkov
  • David Spriggs
  • Tomoko Yaguchi
  • Taiichi Katayama
  • Masaya Tohyama
  • Kamaljit Kaur
  • Yasushi Soda
  • Kenzaburo Tani
  • Shigetaka Asano
  • Shizuka Akieda
  • Yuki Yamasaki
  • Atsushi Maruyama
  • Naruhiko Fukuchi
  • Tsuyoshi Goto
  • Tohru Fushiki
  • Aki Taimatsu
  • Kenshi Hayashi
  • Teruo Kawada
  • Tomoko Tahira
  • Toru Iwaki
  • Won Jong Kim
  • Takayuki Yamamoto
  • Kazunari K Yokoyama
  • Taro Q P Uyeda

Detail Information

Publications37

  1. ncbi LIM kinase-2 targeting as a possible anti-metastasis therapy
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    J Gene Med 6:357-63. 2004
    ..In this study, we have determined the effect of ribozymes specific for LIM kinase-2 on metastatic and proliferative phenotypes of human fibrosarcoma cells...
  2. ncbi Induction of DNA methylation and gene silencing by short interfering RNAs in human cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nature 431:211-7. 2004
    ....
  3. ncbi Transcriptional gene silencing by short interfering RNAs
    Hiroaki Kawasaki
    The University of Tokyo, Department of Chemistry and Biotechnology, School of Engineering, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Curr Opin Mol Ther 7:125-31. 2005
    ..Recently, siRNAs were demonstrated to induce transcriptional gene silencing (TGS) via DNA methylation in human cells. This review will focus on TGS by siRNAs, such as DNA methylation and histone methylation...
  4. ncbi Hes1 is a target of microRNA-23 during retinoic-acid-induced neuronal differentiation of NT2 cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nature 423:838-42. 2003
    ..Thus, our results indicate that miR-23 regulates the expression of Hes1 at the post-transcriptional level, and participates in retinoic-acid-induced neuronal differentiation of NT2 cells...
  5. ncbi Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    J Biol Chem 279:38083-6. 2004
    ..The further characterization of these candidate genes would provide clues to the complex mechanism(s) of metastasis...
  6. ncbi Maxizyme technology
    Mayu Iyo
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
    Methods Mol Biol 252:257-65. 2004
    ..Thus, maxizyme technology is not limited to one specific case, but may have broad general applicability in molecular biology and in molecular gene therapy...
  7. ncbi Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo
    Keisuke Oshima
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113 8656, Japan
    Cancer Res 63:6809-14. 2003
    ..Our findings confirm the potential utility of maxizymes and shRNAs as therapeutic agents...
  8. ncbi Cell migration and metastasis as targets of small RNA-based molecular genetic analyses
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan
    J Muscle Res Cell Motil 25:303-8. 2004
    ..We then describe our promising novel approach that uses randomized ribozyme libraries for identification of genes involved in cell migration, a consistent feature of metastatic cells...
  9. ncbi World of small RNAs: from ribozymes to siRNA and miRNA
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Differentiation 72:58-64. 2004
    ..This article reviews our contributions to the understanding of structure, the function of small RNAs, their use in biotechnology, and the understanding of phenotypes such as apoptosis, metastasis, and differentiation...
  10. ncbi Identification of genes responsible for cell migration by a library of randomized ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    Cancer Res 63:119-24. 2003
    ..Ribozymes identified in this study, including the ribozymes against ROCK1, might be useful in understanding the mechanisms of cell migration and metastasis...
  11. ncbi Functional gene discovery using hybrid ribozyme libraries
    Yoshio Kato
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
    Methods Mol Biol 252:245-56. 2004
    ..This chapter describes a method known as the gene-discovery system to identify novel functional genes related to a specific phenotype. Our gene-discovery system should be a powerful tool for post-genome research...
  12. ncbi siRNA induced transcriptional gene silencing in mammalian cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo, Japan
    Cell Cycle 4:442-8. 2005
    ..e., directed control of a genes expression) and biologically in understanding the evolution of the cell...
  13. pmc Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, Hongo, Tokyo 113 8656, Japan
    Proc Natl Acad Sci U S A 100:5616-21. 2003
    ..Our system for gene identification by using ribozymes and the functional analysis of target genes should help to clarify the complex mechanisms of invasion and metastasis and might provide information that is relevant to cancer therapy...
  14. ncbi Identification of a caspase 3-independent role of pro-apoptotic factor Bak in TNF-alpha-induced apoptosis
    Eigo Suyama
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Japan
    FEBS Lett 528:63-9. 2002
    ..This study demonstrates the caspase 3-independent function of Bak in the TNF-alpha-induced apoptotic pathway...
  15. pmc siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 31:981-7. 2003
    ..However, it is important to avoid homologous sequences within a target mRNA in a given protein family. Our diced siRNA system should be a powerful tool for the inactivation of genes in mammalian cells...
  16. pmc Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Bunkyou ku, Tokyo 113 8656, Japan
    Nucleic Acids Res 31:700-7. 2003
    ..Our tRNA-dsRNA technology should be a powerful tool for studies of the mechanism of RNAi and the functions of various genes in mammalian cells with potential utility as a therapeutic agent...
  17. ncbi [RNAi-mediated specific gene silencing and its application to medical treatments of viral infectious disease]
    Hiroshi Minoshima
    Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Uirusu 53:7-14. 2003
  18. ncbi Construction of microRNA-containing vectors for expression in mammalian cells
    Yoko Fukuda
    Department of Chemistry and Biotechnology, The University of Tokyo, Japan
    Methods Mol Biol 338:167-73. 2006
    ..miRNA expression vectors containing human RNA polymerase II or III promoters, and utilizing a flexible vector system, can be useful for functional analysis...
  19. ncbi Helicase-attached novel hybrid ribozymes
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Japan
    Methods Mol Biol 252:237-43. 2004
    ..Thus, hybrid ribozymes can catalyze cleavage at the specified target site within an RNA in vivo almost independently of the secondary or tertiary structure of the target RNA around the cleavage site...
  20. ncbi Enhancement in the cleavage activity of a hammerhead ribozyme by cationic comb-type polymers and an RNA helicase in vitro
    Hideo Akashi
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    J Biochem 131:687-92. 2002
    ..These approaches should be useful in the development of efficient gene-inactivating reagents in the post-genomic era...
  21. pmc Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicase
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    EMBO Rep 3:443-50. 2002
    ..This application of a randomized library of hybrid ribozymes represents a simple, powerful method for the identification of genes associated with specific phenotypes in the post-genome era...
  22. ncbi Allosterically controllable maxizymes for molecular gene therapy
    Mayu Iyo
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Japan
    Curr Opin Mol Ther 4:154-65. 2002
    ..Thus, maxizyme technology is not limited to a specific case but may have broad general applicability in molecular biology and in molecular gene therapy...
  23. pmc A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    Nucleic Acids Res 30:3609-14. 2002
    ....
  24. ncbi Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme libraries
    Hiroaki Kawasaki
    Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113 8656, Japan
    Nat Biotechnol 20:376-80. 2002
    ..We identified many pro-apoptotic genes and partial sequences of previously uncharacterized genes using this method. Our gene discovery system should be generally applicable to the identification of functional genes in various systems...
  25. ncbi Lysophosphatidic acid acyltransferase-beta is a prognostic marker and therapeutic target in gynecologic malignancies
    Gregory M Springett
    Memorial Sloan Kettering Cancer Center, Developmental Chemotherapy Laboratory, Department of Medicine, New York, New York corrected USA
    Cancer Res 65:9415-25. 2005
    ..These findings identify LPAAT-beta as a potential prognostic and therapeutic target in ovarian and endometrial cancer...
  26. ncbi Characterization of a hemolytic protein, identified as histone H4, from the skin of the Japanese tree frog Hyla japonica (Hylidae)
    Hiroaki Kawasaki
    Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274 8510, Japan
    Comp Biochem Physiol B Biochem Mol Biol 149:120-5. 2008
    ..japonica histone H4 gene, which acts as a transcription termination signal, contained a nucleotide sequence (5'-GGCTCTCCTCAGAGCC-3') with unusual structural features not seen in other histone genes...
  27. ncbi Overexpression and ribozyme-mediated targeting of transcriptional coactivators CREB-binding protein and p300 revealed their indispensable roles in adipocyte differentiation through the regulation of peroxisome proliferator-activated receptor gamma
    Nobuyuki Takahashi
    Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    J Biol Chem 277:16906-12. 2002
    ..These data suggest that both CBP and p300 are indispensable for the full activation of PPARgamma and adipocyte differentiation and that CBP and p300 do not mutually complement in the process...
  28. ncbi Analysis of a mitochondrial apoptotic pathway using Bid-targeted ribozymes in human MCF7 cells in the absence of a caspase-3-dependent pathway
    Reiko Onuki
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology AIST, Central 4, Higashi, Tsukuba Science City 305 8562, Japan
    Antisense Nucleic Acid Drug Dev 13:75-82. 2003
    ..Therefore, it became clear for the first time that caspase 9 by itself can activate caspase 7 in the absence of the caspase 3-dependent pathway in TNF-alpha-induced apoptosis by the use of specific ribozymes...
  29. pmc Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes
    Renu Wadhwa
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, 1 1 1 Higashi, Tsukuba, Ibaraki, 305 8566, Japan
    EMBO Rep 4:595-601. 2003
    ....
  30. pmc An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease
    Reiko Onuki
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology, Higashi, Tsukuba Science City, Japan
    EMBO J 23:959-68. 2004
    ..We also detected the accumulation of phosphorylated PKR in the nuclei of autopsied brain tissues in Alzheimer's disease. Thus, PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease...
  31. ncbi Antimicrobial peptides from the skin of the Japanese mountain brown frog Rana ornativentris: evidence for polymorphism among preprotemporin mRNAs
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Peptides 28:524-32. 2007
    ....
  32. ncbi Down-regulation of endogenous Wt1 expression by Sry transgene in the murine embryonic mesonephros-derived M15 cell line
    Masanori Ito
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
    J Reprod Dev 52:415-27. 2006
    ..e., turning on Sry expression on one hand, and being down-regulated by its product, Sry, on the other, possibly forming a type of negative feed-back mechanism. Further work is needed to substantiate this view...
  33. ncbi Developmental and triiodothyronine-induced expression of genes encoding preprotemporins in the skin of Tago's brown frog Rana tagoi
    Aya Ohnuma
    Department of Biology, Faculty of Science, Toho University, 2 2 1 Miyama, Funabashi, Chiba 274 8510, Japan
    Gen Comp Endocrinol 146:242-50. 2006
    ....
  34. pmc Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation
    Reiko Onuki
    Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology, Central 4, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    Proc Natl Acad Sci U S A 99:14716-21. 2002
    ....
  35. ncbi [Development of siRNA libraries by in vitro dicing and optimized efficient expression vectors for siRNAs in mammalian cells]
    Hiroaki Kawasaki
    Tanpakushitsu Kakusan Koso 48:1638-45. 2003
  36. ncbi A protein with antimicrobial activity in the skin of Schlegel's green tree frog Rhacophorus schlegelii (Rhacophoridae) identified as histone H2B
    Hiroaki Kawasaki
    Department of Biology, Faculty of Science, Toho University, Funabashi, 274 8510, Chiba, Japan
    Biochem Biophys Res Commun 312:1082-6. 2003
    ..The data provide further evidence that histones play a role in the defense against microorganisms...
  37. ncbi Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)
    Shinji Fukuda
    Division of Genome Analysis, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biochem 131:349-57. 2002
    ..The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells...