Hiroshi Kasai

Summary

Affiliation: University of Occupational and Environmental Health
Country: Japan

Publications

  1. ncbi request reprint Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis
    H Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Mutat Res 387:147-63. 1997
  2. ncbi request reprint Chemistry-based studies on oxidative DNA damage: formation, repair, and mutagenesis
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Free Radic Biol Med 33:450-6. 2002
  3. doi request reprint DNA methylation at the C-5 position of cytosine by methyl radicals: a possible role for epigenetic change during carcinogenesis by environmental agents
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu, 807 8555, Japan
    Chem Res Toxicol 22:984-9. 2009
  4. ncbi request reprint A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University, of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J Radiat Res 44:185-9. 2003
  5. ncbi request reprint Detection of a smaller, 32-kDa 8-oxoguanine DNA glycosylase 1 in 3'-methyl-4-dimethylamino-azobenzene-treated mouse liver
    Takeshi Hirano
    Department of Environmental Oncology, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu, Fukuoka 807 8555, Japan
    Cancer Sci 95:118-22. 2004
  6. ncbi request reprint Life style and urinary 8-hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking
    H Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Jpn J Cancer Res 92:9-15. 2001
  7. ncbi request reprint Analyses of oxidative DNA damage and its repair activity in the livers of 3'-methyl-4-dimethylaminoazobenzene-treated rodents
    T Hirano
    Department of 1 Environmental Oncology, University of Occupational and Environmental Health, Japan
    Jpn J Cancer Res 91:681-5. 2000
  8. doi request reprint 4-oxo-2-hexenal, a mutagen formed by omega-3 fat peroxidation: occurrence, detection and adduct formation
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Mutat Res 659:56-9. 2008
  9. doi request reprint 8-Hydroxyguanine levels and repair capacity during mouse embryonic stem cell differentiation
    Ayumi Kuboyama
    Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka, 808 0135, Japan
    Free Radic Res 45:527-33. 2011
  10. ncbi request reprint Effect of age, smoking and other lifestyle factors on urinary 7-methylguanine and 8-hydroxydeoxyguanosine
    Kazuyoshi Tamae
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
    Cancer Sci 100:715-21. 2009

Detail Information

Publications76

  1. ncbi request reprint Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis
    H Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Mutat Res 387:147-63. 1997
    ..The validity of the general use of 8-OH-dG as a marker of cellular oxidative stress is discussed...
  2. ncbi request reprint Chemistry-based studies on oxidative DNA damage: formation, repair, and mutagenesis
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Free Radic Biol Med 33:450-6. 2002
    ..By these studies, we found solid chemistry-based approaches were often useful to clarify the biological phenomena...
  3. doi request reprint DNA methylation at the C-5 position of cytosine by methyl radicals: a possible role for epigenetic change during carcinogenesis by environmental agents
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu, 807 8555, Japan
    Chem Res Toxicol 22:984-9. 2009
    ..We propose here that this reaction is one of the important mechanisms of de novo DNA methylation during carcinogenesis, because methyl radicals are produced by the biotransformation of various endogenous and exogenous compounds...
  4. ncbi request reprint A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detector system
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University, of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J Radiat Res 44:185-9. 2003
    ..Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion...
  5. ncbi request reprint Detection of a smaller, 32-kDa 8-oxoguanine DNA glycosylase 1 in 3'-methyl-4-dimethylamino-azobenzene-treated mouse liver
    Takeshi Hirano
    Department of Environmental Oncology, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu, Fukuoka 807 8555, Japan
    Cancer Sci 95:118-22. 2004
    ..Although the function of mOGG1-32 remains unclear, these findings suggest that 3'-MeDAB may alter the function of the DNA repair protein, and this action may be related to 3'-MeDAB carcinogenesis...
  6. ncbi request reprint Life style and urinary 8-hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking
    H Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Jpn J Cancer Res 92:9-15. 2001
    ..013), and day-night shift work (P = 0.044) increased its level. These results suggest that many types of life-style factors that either generate or scavenge oxygen radicals may affect the level of oxidative DNA damage of each individual...
  7. ncbi request reprint Analyses of oxidative DNA damage and its repair activity in the livers of 3'-methyl-4-dimethylaminoazobenzene-treated rodents
    T Hirano
    Department of 1 Environmental Oncology, University of Occupational and Environmental Health, Japan
    Jpn J Cancer Res 91:681-5. 2000
    ..6-fold and 1.6-fold, respectively. These experiments suggest that 3'-MeDAB increases 8-OH-Gua generation in rodent liver DNA and the 8-OH-Gua repair assay is a reliable marker of cellular oxidative stress induced by carcinogens...
  8. doi request reprint 4-oxo-2-hexenal, a mutagen formed by omega-3 fat peroxidation: occurrence, detection and adduct formation
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Mutat Res 659:56-9. 2008
    ..Since 4-OHE induces DNA adduct formation in experimental animal organs, further studies on the carcinogenicity of 4-OHE and the detection of 4-OHE-DNA adducts in human tissue will be required...
  9. doi request reprint 8-Hydroxyguanine levels and repair capacity during mouse embryonic stem cell differentiation
    Ayumi Kuboyama
    Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka, 808 0135, Japan
    Free Radic Res 45:527-33. 2011
    ..Taken together, ES cells are more resistant to DNA oxidative stresses than differentiated cells...
  10. ncbi request reprint Effect of age, smoking and other lifestyle factors on urinary 7-methylguanine and 8-hydroxydeoxyguanosine
    Kazuyoshi Tamae
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
    Cancer Sci 100:715-21. 2009
    ..Therefore, the urinary m7Gua level is considered to be a useful marker of DNA methylation, not only from smoking, but also from aging and unhealthy dietary habits...
  11. ncbi request reprint Identification of 4-oxo-2-hexenal and other direct mutagens formed in model lipid peroxidation reactions as dGuo adducts
    Muneyuki Maekawa
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health UOEH, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Chem Res Toxicol 19:130-8. 2006
    ..We confirmed the mutagenic activity of 4-OHE with Salmonella strains, TA100 and TA104, without S9 mix. These compounds may be involved in lipid peroxide-related cancers...
  12. ncbi request reprint Identification of octenal-related dA and dC adducts formed by reactions with a hemin-ω-6-fat peroxidation model system
    Kazuaki Kawai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Chem Res Toxicol 26:1554-60. 2013
    ..These mutagens may be formed during storage and cooking of food, or during digestion, and may be involved in human cancers...
  13. doi request reprint DNA modifications by the omega-3 lipid peroxidation-derived mutagen 4-oxo-2-hexenal in vitro and their analysis in mouse and human DNA
    Kazuaki Kawai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Chem Res Toxicol 23:630-6. 2010
    ..6-5.9/10(9) bases. No difference in the 4-OHE adduct levels was detected between smokers and nonsmokers. Our results suggest that 4-OHE-DNA adducts are formed by endogenous as well as environmental lipid peroxides...
  14. ncbi request reprint Analysis of 8-hydroxyguanine (8-OH-Gua) released from DNA by the formamidopyrimidine DNA glycosylase (Fpg) protein: a reliable method to estimate cellular oxidative stress
    Hiroshi Orimo
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    J Radiat Res 45:455-60. 2004
    ..With our new analysis method, it is possible to detect the small changes in the 8-OH-Gua levels in cellular DNA induced by various environmental factors...
  15. ncbi request reprint Urinary 8-hydroxyguanine may be a better marker of oxidative stress than 8-hydroxydeoxyguanosine in relation to the life spans of various species
    Peter Svoboda
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Antioxid Redox Signal 8:985-92. 2006
    ..91, p = 0.01) or 8- OH-Gua/creatinine (r = 0.90, p = 0.01). These results suggest that 8-OH-Gua, rather than 8-OH-dG, may be a more general marker for oxidative damage...
  16. doi request reprint Chronic alcohol consumption prevents 8-hydroxyguanine accumulation in 3'-methyl-4-dimethylaminoazobenzene-treated mouse liver
    Takeshi Hirano
    Department of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka 808 0135, Japan
    Biochem Biophys Res Commun 387:316-20. 2009
    ..These findings suggested that 12% ethanol consumption may reduce the risk of 3'-MeDAB-induced carcinogenesis by decreasing 8-OH-Gua accumulation...
  17. doi request reprint Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG
    Ming Fen Song
    Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu 807 8555, Japan
    Free Radic Biol Med 47:41-6. 2009
    ..In addition, performance of the ELISA at 4 degrees C reduced the recognition of urea considerably and improved the 8-OH-dG analysis...
  18. ncbi request reprint 4-oxo-2-hexenal, a mutagen formed by omega-3 fat peroxidation, causes DNA adduct formation in mouse organs
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Ind Health 43:699-701. 2005
    ..The 4-OHE seems to be produced by the auto-oxidation of omega-3 polyunsaturated fatty acids. These results provide a warning to workers dealing with omega-3 fats, who may be exposed to this volatile mutagen...
  19. ncbi request reprint Increase of human MTH1 and decrease of 8-hydroxydeoxyguanosine in leukocyte DNA by acute and chronic exercise in healthy male subjects
    Yuji Sato
    Department of Health Development, University of Occupational and Environmental Health, Iseigaoka, Yahata Nishiku, Kitakyushu 807 8555, Japan
    Biochem Biophys Res Commun 305:333-8. 2003
    ..These results suggest that a mild exercise has a beneficial effect on the maintenance of low levels of 8-OH-dG probably by keeping the sanitization system at higher levels...
  20. ncbi request reprint Formation of 8-hydroxy-2'-deoxyguanosine in the DNA of cultured human keratinocytes by clinically used doses of narrowband and broadband ultraviolet B and psoralen plus ultraviolet A
    Hiroshi Orimo
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Cancer Sci 97:99-105. 2006
    ..Our study provides a warning that widely used narrowband UVB and PUVA induce cellular oxidative DNA damage at the therapeutically used doses, although to a lesser degree than broadband UVB with the same clinically effective dose...
  21. doi request reprint Effect of polymerized toner on rat lung in chronic inhalation study
    Yasuo Morimoto
    Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Inhal Toxicol 21:898-905. 2009
    ..A significant production of 8-OHdG was not observed in the toner exposed groups. In conclusion, toner produced by polymerization was not associated with evidence of carcinogenesis in this experiment...
  22. ncbi request reprint Detection of a mouse OGG1 fragment during caspase-dependent apoptosis: oxidative DNA damage and apoptosis
    Takeshi Hirano
    Department of Environmental Oncology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu, Fukuoka 807 8555, Japan
    Cancer Sci 95:634-8. 2004
    ....
  23. ncbi request reprint Analysis of 8-hydroxydeoxyguanosine 5'-monophosphate (8-OH-dGMP) as a reliable marker of cellular oxidative DNA damage after gamma-irradiation
    Nan Mei
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan
    Environ Mol Mutagen 41:332-8. 2003
    ..Using an endonuclease nicking assay, we also found that gamma-rays decreased 8-OH-Gua repair activity. The results indicate that 8-OH-dGMP is a useful and sensitive marker for estimating oxidative damage in DNA...
  24. ncbi request reprint 8-hydroxyguanine in urine and serum as an oxidative stress marker: effects of diabetes and aging
    Yun Shan Li
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan
    J UOEH 35:119-27. 2013
    ....
  25. ncbi request reprint Simultaneous determination of 8-hydroxydeoyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine
    Hiroshi Kasai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Ind Health 43:333-6. 2005
    ..In this communication, we report a method for the simultaneous determination of 8-OH-dG and creatinine, an internal standard for normalizing the excretion of 8-OH-dG in urine...
  26. doi request reprint 8-hydroxydeoxyguanosine generated in the earthworm Eisenia fetida grown in metal-containing soil
    Tamiji Nakashima
    Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, Japan, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu, Fukuoka 807 8555, Japan
    Mutat Res 654:138-44. 2008
    ..Although some points remain unresolved, a bio-monitoring system analyzing the DNA damage generated in the earthworm might be useful for the assessment of the mutagenicity of soil contaminated with various heavy metals, such as Cd...
  27. ncbi request reprint Relations between clusters of oxidatively damaged nucleotides and active or open nucleosomes in the rat Nth 1 gene
    Minoru Nomoto
    Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Japan, Yahata nishi ku, Kitakyushu 807 8555, Japan
    Oncogene 21:1649-57. 2002
    ..Taking into account the function of the protein segment encoded by these regions, we discussed the molecular mechanism of the restricted formation of the damaged nucleotides...
  28. doi request reprint DNA methylation by dimethyl sulfoxide and methionine sulfoxide triggered by hydroxyl radical and implications for epigenetic modifications
    Kazuaki Kawai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Bioorg Med Chem Lett 20:260-5. 2010
    ..In addition to m(5)dC, the formation of 8-methyldeoxyguanosine (m(8)dG) was also detected in DNA under the same reaction conditions. The m(8)dG level in human DNA may be a useful indicator of DNA methylation by radical mechanisms...
  29. ncbi request reprint Effect of work conditions and work environments on the formation of 8-OH-dG in nurses and non-nurse female workers
    Itsuko Ishihara
    Department of Public Health and Occupational Health Nursing, School of Health Sciences, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J UOEH 30:293-308. 2008
    ..32, P < 0.05). Together, it was suggested that a heavy burden of work in addition to daily domestic roles increases urinary excretion levels of 8-OH-dG...
  30. ncbi request reprint The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein
    Katsuyoshi Fujikawa
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    DNA Repair (Amst) 1:571-6. 2002
    ..This result indicates that 5-hydroxy-CTP is the best substrate of Orf135 among the nucleotides tested...
  31. ncbi request reprint Detection of 4-oxo-2-hexenal, a novel mutagenic product of lipid peroxidation, in human diet and cooking vapor
    Kazuaki Kawai
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Mutat Res 603:186-92. 2006
    ..The 4-OHE may be produced from omega-3 polyunsaturated fats, such as alpha-linolenic acid-, docosahexaenoic acid (DHA)- and eicosapentaenoic acid (EPA)-TG, which are more easily oxidized than omega-6 fats, such as linoleic acid-TG...
  32. ncbi request reprint Acute arsenite-induced 8-hydroxyguanine is associated with inhibition of repair activity in cultured human cells
    Nan Mei
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807 8555, Japan
    Biochem Biophys Res Commun 297:924-30. 2002
    ....
  33. ncbi request reprint Negative effect of long-term inhalation of toner on formation of 8-hydroxydeoxyguanosine in DNA in the lungs of rats in vivo
    Yasuo Morimoto
    Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan
    Inhal Toxicol 17:749-53. 2005
    ..Inhalation of the toner for 1 and even 2 yr did not induce the formation of 8-OH-Gua in DNA in rat lungs. These data suggest that long-term inhalation of toner may not induce lung tumors...
  34. pmc Metal nanoparticle-induced micronuclei and oxidative DNA damage in mice
    Ming Fen Song
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1, Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J Clin Biochem Nutr 50:211-6. 2012
    ..In conclusion, the metal nanoparticles caused genotoxicity, and oxidative stress may be responsible for the toxicity of these metal nanoparticles...
  35. ncbi request reprint Simultaneous HPLC analysis of 8-hydroxydeoxyguanosine and 7-methylguanine in urine from humans and rodents
    Peter Svoboda
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Anal Biochem 334:239-50. 2004
    ....
  36. ncbi request reprint Psychological mediation of a type of oxidative DNA damage, 8-hydroxydeoxyguanosine, in peripheral blood leukocytes of non-smoking and non-drinking workers
    Masahiro Irie
    Department of Mental Health, Institute of Industrial Ecological Sciences, Kitakyushu, Japan
    Psychother Psychosom 71:90-6. 2002
    ....
  37. pmc Circadian disruption accelerates tumor growth and angio/stromagenesis through a Wnt signaling pathway
    Yoshihiro Yasuniwa
    Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan
    PLoS ONE 5:e15330. 2010
    ..These findings suggest that circadian disruption induces the progression of malignant tumors via a Wnt signaling pathway...
  38. ncbi request reprint A very low level of magnetic field exposure does not affect a participant's mental fatigue and stress as much as VDT work
    Itsuko Ishihara
    Department of Public Health and Occupational Health Nursing, School of Health Sciences, University of Occupational and Environmental Health, Japan, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J UOEH 27:25-40. 2005
    ..Taken together, the results of this study did not detect any bad health effects of MF exposure on the VDT participants but suggested that VDT work itself affects their mental fatigue and psycho-physiological status...
  39. ncbi request reprint Human mitochondrial transcription factor A binds preferentially to oxidatively damaged DNA
    Yoichiro Yoshida
    Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu, Fukuoka, Japan
    Biochem Biophys Res Commun 295:945-51. 2002
    ..The affinity for oxidatively damaged DNA of mtTFA is higher for A/8-oxo-dG and C/8-oxo-dG than for G/8-oxo-dG and T/8-oxo-dG. Our findings suggest that mtTFA plays an important role in the recognition of oxidative DNA damage...
  40. ncbi request reprint Chronic arsenic exposure and urinary 8-hydroxy-2'-deoxyguanosine in an arsenic-affected area in Inner Mongolia, China
    Yoshihisa Fujino
    Department of Clinical Epidemiology, Institute of Industrial Ecological Science, University of Occupational and Environmental Health, Japan
    J Expo Anal Environ Epidemiol 15:147-52. 2005
    ..The purpose of this study was to examine the relationship between arsenic in drinking water and oxidative stress in humans by measuring 8-Hydroxy-2'-deoxyguanosine (8-OHdG)...
  41. ncbi request reprint Urinary 8-hydroxydeoxyguanosine levels and psychological reactions after sleep deprivation
    Kazunori Ikegami
    Department of Mental Health, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan Yahatanishi ku, Kitakyushu 807 8555, Japan
    J UOEH 32:1-10. 2010
    ..These results suggest that the effect of one night of SD on psychological reactions continued for 2 or 3 days, and SD might not influence urinary 8-OH-dG levels despite marked changes in psychological reactions...
  42. doi request reprint Cross-sectional study on respiratory effect of toner exposure
    Niina Terunuma
    Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Japan, Kitakyusyu, Japan
    Hum Exp Toxicol 28:325-30. 2009
    ..It should also be noted that this tendency did not exceed that of the general public. Further analysis is required in this ongoing 10-year cohort study to clarify the effect of toner exposure on the respiratory system...
  43. ncbi request reprint 8-Chloro-dGTP, a hypochlorous acid-modified nucleotide, is hydrolyzed by hMTH1, the human MutT homolog
    Katsuyoshi Fujikawa
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, 807 8555, Kitakyushu, Japan
    FEBS Lett 512:149-51. 2002
    ..The kinetic parameters revealed that 8-chloro-dGTP was degraded by hMTH1 with 50% efficiency as compared with that of 8-hydroxy-dGTP. This result suggests that 8-chloro-dGTP is an intrinsic substrate for hMTH1...
  44. ncbi request reprint Induction of T --> G and T --> A transversions by 5-formyluracil in mammalian cells
    Hiroyuki Kamiya
    Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    Mutat Res 513:213-22. 2002
    ..C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells...
  45. ncbi request reprint Habitual exercise induced resistance to oxidative stress
    Koji Nakatani
    Department of Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima, 890 8544, Japan
    Free Radic Res 39:905-11. 2005
    ..These results suggest that HE induces resistance to oxidative stress and, at least at the initiation stage, inhibits carcinogenesis...
  46. ncbi request reprint UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides
    Mika Hori
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 6:1786-93. 2007
    ..Thus, UvrA and UvrB are involved in the enhancement, but not in the suppression, of the mutations induced by these oxidized deoxyribonucleotides. These results suggest a novel role for UvrA and UvrB in the processing of oxidative damage...
  47. ncbi request reprint Lack of initiation activity of 4-oxo-2-hexenal, a peroxidation product generated from omega-3 polyunsaturated fatty acids, in an in vivo five-week liver assay
    Shinji Takasu
    Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464 8681, Japan
    Asian Pac J Cancer Prev 8:372-4. 2007
    ..78?1.33). Areas of GST-P positive foci were also similar (1.11-/+0.5 and 1.53-/+1.33 mm2/cm2 in "128" and the control groups, respectively). These results showed 4-OHE to have no significant initiation activity in...
  48. ncbi request reprint Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells
    Kazuya Satou
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    Free Radic Biol Med 42:1552-60. 2007
    ..coli. These results constitute the first direct evidence to show that 8-OH-dGTP actually induces mutations in living mammalian cells...
  49. ncbi request reprint Comparison between high-performance liquid chromatography and enzyme-linked immunosorbent assay for the determination of 8-hydroxy-2'-deoxyguanosine in human urine
    Kayoko Shimoi
    Institute for Environmental Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422 8526
    Cancer Epidemiol Biomarkers Prev 11:767-70. 2002
    ..It is suggested that the ELISA system is applicable for comparative human monitoring studies. Prepurification of 'samples is required to determine the absolute value of 8-OH-dG in individual urine samples by ELISA...
  50. ncbi request reprint Analysis of 8-hydroxydeoxyguanosine among workers exposed to diesel particulate exhaust: comparison with urinary metabolites and PAH air monitoring
    Minna Harri
    Finnish Institute of Occupational Health, Helsinki, Finland
    Free Radic Res 39:963-72. 2005
    ..01, n=28, P=0.96). The PAH exposure at workplaces was mainly composed of volatile compounds, particularly naphthalene, suggesting low exposure through the respiratory tract and a low effect of PAH in ROS induction...
  51. ncbi request reprint Occupational and lifestyle factors and urinary 8-hydroxydeoxyguanosine
    Masahiro Irie
    Institute of Health Science, Kyushu University, 6 1 Kasuga Park, Kasuga, Fukuoka 816 8580, Japan
    Cancer Sci 96:600-6. 2005
    ..We suggest that several occupational and lifestyle factors, particularly long working hours and cigarette smoking, are linked to the formation of 8-OH-dG in workers...
  52. ncbi request reprint Prospective study of 8-oxo-7,8-dihydro-2'-deoxyguanosine excretion and the risk of lung cancer
    Steffen Loft
    Department of Environmental and Occupational Health, University of Copenhagen, Denmark
    Carcinogenesis 27:1245-50. 2006
    ..8 (1.21-115) per doubling of 8-oxodG excretion. The association between 8-oxodG excretion and lung cancer risk among never-smokers suggests that oxidative damage to DNA nucleotides is important in this group...
  53. ncbi request reprint Changes of 8-OH-dG levels in DNA and its base excision repair activity in rat lungs after inhalation exposure to hexavalent chromium
    Seung Hee Maeng
    Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency, Daejeon 305 380, South Korea
    Mutat Res 539:109-16. 2003
    ..However, the 8-OH-dG levels and its repair activities recovered to the level of the controls in the latter inhalation exposure period...
  54. ncbi request reprint Oxidative DNA damage: mechanisms and significance in health and disease
    Hiroshi Kasai
    Antioxid Redox Signal 8:981-3. 2006
  55. ncbi request reprint Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine values measured by an ELISA correlated well with measurements by high-performance liquid chromatography with electrochemical detection
    Rie Yoshida
    National Institute of Industrial Health, Kawasaki 214 8585, Japan
    Cancer Epidemiol Biomarkers Prev 11:1076-81. 2002
    ..These results show that the ELISA can be applied to studies comparing relative urinary 8-oxo-dG values among several groups, if the studies do not require determination of the exact concentration of 8-oxo-dG in urine...
  56. pmc Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis
    Shinya Akatsuka
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Am J Pathol 169:1328-42. 2006
    ..This versatile technique would introduce a novel research area as a high-throughput screening method for critical genomic loci under oxidative stress...
  57. pmc An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates
    Toshinori Kai
    Graduate School of Science and Technology, Faculty of Science, Kumamoto University, 2 39 1 Kurokami, Kumamoto 860 8555, Japan
    Nucleic Acids Res 30:569-73. 2002
    ..Therefore, the mechanism of delay of DNA synthesis by 8-oxo-dGTP may be different from that by UV lesions. This is the first report that demonstrates an effect of an oxidized nucleotide on DNA replication in eukaryotes...
  58. ncbi request reprint UVA1 genotoxicity is mediated not by oxidative damage but by cyclobutane pyrimidine dimers in normal mouse skin
    Hironobu Ikehata
    Department of Cell Biology, Graduate School of Medicine, Tohoku University, Sendai, Japan
    J Invest Dermatol 128:2289-96. 2008
    ....
  59. ncbi request reprint Induction of OGG1 gene expression by HIV-1 Tat
    Kenichi Imai
    Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya, Aichi 467 8601
    J Biol Chem 280:26701-13. 2005
    ..These findings collectively indicate the possibility that Tat may play a role in maintenance of the genetic integrity of the proviral and host cellular genomes by up-regulating OGG1 as a feed-forward mechanism...
  60. ncbi request reprint The stability of the oxidative stress marker, urinary 8-hydroxy-2'- deoxyguanosine (8-OHdG), when stored at room temperature
    Yuki Matsumoto
    National Institute of Occupational Safety and Health, Kawasaki, Japan
    J Occup Health 50:366-72. 2008
    ..We conclude that urine samples can be stored at 25 degrees C and below for 24 h, when the research purpose includes the determination of urinary 8-OHdG. Urinary 8-OHdG was also stable for over two years when stored at -80 degrees C...
  61. ncbi request reprint Evaluation of oxidative stress in a group of adolescents exposed to a high level of aflatoxin B1--a multi-center and multi-biomarker study
    Tao Peng
    Department of Hepatobiliary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China
    Carcinogenesis 28:2347-54. 2007
    ..These findings suggest significant contribution from AFB1 exposure to oxidative stress and subsequent repair among adolescents that may impose substantial risk for hepatocarcinogenesis in adulthood in this region...
  62. ncbi request reprint Dual hydrolysis of diphosphate and triphosphate derivatives of oxidized deoxyadenosine by Orf17 (NtpA), a MutT-type enzyme
    Mika Hori
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 4:33-9. 2005
    ..These results suggest that the Orf17 protein may be involved in the hydrolysis of oxidized dATP and dADP...
  63. ncbi request reprint Depressive state relates to female oxidative DNA damage via neutrophil activation
    Masahiro Irie
    Institute of Health Science, Kyushu University, 6 1 Kasuga Park, Kasuga, 816 8580, Fukuoka, Japan
    Biochem Biophys Res Commun 311:1014-8. 2003
    ..There was a positive correlation between the percentage of neutrophils and the 8-OH-dG levels in females. Psychological depression was related to cancer risk due to oxidative DNA damage in females, possibly via neutrophil activation...
  64. ncbi request reprint Depression and possible cancer risk due to oxidative DNA damage
    Masahiro Irie
    Second Department of Health Sciences Clinical Science and Psychology, Institute of Health Science, Kyushu University, Kasuga, Fukuoka 816 8580, Japan
    J Psychiatr Res 39:553-60. 2005
    ..This study suggests that clinical depression is a risk factor for cancer initiation in view of oxidative DNA damage...
  65. ncbi request reprint Negative association between serum dioxin level and oxidative DNA damage markers in municipal waste incinerator workers
    Jin Yoshida
    Department of Environmental Health, Osaka Prefectural Institute of Public Health, Osaka, 1 3 69, Nakamichi, Higashinari ku, 537 0025, Japan
    Int Arch Occup Environ Health 79:115-22. 2006
    ....
  66. ncbi request reprint Suppression of hyperemia and DNA oxidation by indomethacin in cerebral ischemia
    Osamu Miyamoto
    Department of Neurobiology, Kagawa Medical University, 1750 1, Ikenobe, Miki cho, Kita gun, Kagawa 761 0793, Japan
    Eur J Pharmacol 459:179-86. 2003
    ..05). These results suggest that indomethacin may protect neurons by attenuating oxidative stress and reperfusion injury in ischemic insult...
  67. ncbi request reprint Leanness, smoking, and enhanced oxidative DNA damage
    Tetsuya Mizoue
    Department of Preventive Medicine, Faculty of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashiku, 812 8582 Fukuoka, Japan
    Cancer Epidemiol Biomarkers Prev 15:582-5. 2006
    ..The interaction of smoking and BMI reached statistical significance (P = 0.04). Leanness may enhance oxidative DNA damage induced by smoking and thus serve as a marker of host susceptibility to smoking-related cancers...
  68. ncbi request reprint Absence of in vivo genotoxic potential and tumor initiation activity of kojic acid in the rat thyroid
    Toru Tamura
    Division of Pathology, National Institute of Health Sciences, 1 18 1, Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Toxicology 222:213-24. 2006
    ....
  69. ncbi request reprint [Relationship between length of sleep and oxidative stress marker, urinary 8-hydroxy-2'-deoxyguanosine]
    Yuki Matsumoto
    National Institute of Industrial Health, Kawasaki
    Nihon Eiseigaku Zasshi 61:357-65. 2006
    ..In this study, we aim to examine whether the length of sleep modifies the level of urinary 8-hydroxy-2'-deoxyguanosine (8-OH-dG)...
  70. ncbi request reprint Analysis of 8-hydroxy-2'-deoxyguanosine in urine using high-performance liquid chromatography-electrospray tandem mass spectrometry
    Minna Harri
    Finnish Institute of Occupational Health, Topeliuksenkatu 41 aA, 00250 Helsinki, Finland
    J Chromatogr B Analyt Technol Biomed Life Sci 853:242-6. 2007
    ..49 to 14.27 microg of 8-OHdG/g creatinine. The correlation between the developed HPLC-MS/MS method and the existing HPLC-EC method was good with an R2 value of 0.8707...
  71. ncbi request reprint 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract
    Kazuya Satou
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 46:6639-46. 2007
    ..These results highlight the importance of specific hydrolysis of 8-OH-dGTP for the suppression of its induced mutation...
  72. ncbi request reprint Body mass index and oxidative DNA damage: a longitudinal study
    Tetsuya Mizoue
    Department of Epidemiology and International Health, Research Institute, International Medical Center of Japan, Tokyo 162 8655, Japan
    Cancer Sci 98:1254-8. 2007
    ..7% increase). The results based on a longitudinal observation suggest that weight loss is associated with increased oxidative DNA damage, a state presumably related to an increased risk of cancer...
  73. ncbi request reprint Prospective study of urinary excretion of 7-methylguanine and the risk of lung cancer: Effect modification by mu class glutathione-S-transferases
    Steffen Loft
    Department of Environmental and Occupational Health, University of Copenhagen, Denmark
    Int J Cancer 121:1579-84. 2007
    ..The finding of an association between m(7)Gua excretion and lung cancer risk mainly among current smokers and subjects with GSTM1 null genotype supports causality in this respect...
  74. ncbi request reprint Increased levels of 8-hydroxy-2'-deoxyguanosine in Drosophila larval DNA after irradiation with 364-nm laser light but not with X-rays
    Tomoe Negishi
    Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima, Okayama, Japan
    Photochem Photobiol 83:658-63. 2007
    ..The overall results suggest significant differences in the major in vivo oxidative damage caused by 364-nm light and X-rays...
  75. ncbi request reprint Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription
    Hiroyuki Kamiya
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Free Radic Biol Med 43:837-43. 2007
    ..8-OH-GTP induced T-->G plus T-->C mutations, and 2-OH-ATP caused T-->C mutations. These results indicate that the formation of these oxidized RNA precursors in cells affects transcription quantitatively and qualitatively...
  76. ncbi request reprint Suppression of chemically induced and spontaneously occurring oxidative mutagenesis by three alleles of human OGG1 gene encoding 8-hydroxyguanine DNA glycosylase
    Su Ryang Kim
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Mutat Res 554:365-74. 2004
    ..These results suggest that three alleles of the hOGG1 gene efficiently suppress chemically induced and spontaneously occurring oxidative mutagenesis, and that hOGG1-Gln46 may have a weaker ability to suppress the mutations...