Haruo Kasai

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. pmc Spatial distributions of GABA receptors and local inhibition of Ca2+ transients studied with GABA uncaging in the dendrites of CA1 pyramidal neurons
    Yuya Kanemoto
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    PLoS ONE 6:e22652. 2011
  2. doi request reprint Distinct initial SNARE configurations underlying the diversity of exocytosis
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Physiol Rev 92:1915-64. 2012
  3. ncbi request reprint Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization
    Haruo Kasai
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Adv Drug Deliv Rev 58:850-77. 2006
  4. pmc Two components of exocytosis and endocytosis in phaeochromocytoma cells studied using caged Ca2+ compounds
    H Kasai
    Department of Physiology, Faculty of Medicine, University of Tokyo, Japan
    J Physiol 494:53-65. 1996
  5. doi request reprint Learning rules and persistence of dendritic spines
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, and Center for NanoBio Integration, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Eur J Neurosci 32:241-9. 2010
  6. doi request reprint Structural dynamics of dendritic spines in memory and cognition
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, and Center for NanoBio Integration, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Trends Neurosci 33:121-9. 2010
  7. doi request reprint Exocytosis in islet beta-cells
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, The University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Adv Exp Med Biol 654:305-38. 2010
  8. pmc Multiple kinetic components and the Ca2+ requirements of exocytosis
    H Kasai
    Department of Physiology, Faculty of Medicine, University of Tokyo, Japan
    Philos Trans R Soc Lond B Biol Sci 354:331-5. 1999
  9. ncbi request reprint Hepatocyte nuclear factor-4alpha is essential for glucose-stimulated insulin secretion by pancreatic beta-cells
    Atsuko Miura
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:5246-57. 2006
  10. doi request reprint SNARE conformational changes that prepare vesicles for exocytosis
    Noriko Takahashi
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell Metab 12:19-29. 2010

Collaborators

Detail Information

Publications54

  1. pmc Spatial distributions of GABA receptors and local inhibition of Ca2+ transients studied with GABA uncaging in the dendrites of CA1 pyramidal neurons
    Yuya Kanemoto
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    PLoS ONE 6:e22652. 2011
    ..Our data indicate that GABA inhibition results in spatially confined inhibition of Ca(2+) transients shortly after bAP, and suggest that this effect is particularly potent at the dendritic branch points where GABA receptors cluster...
  2. doi request reprint Distinct initial SNARE configurations underlying the diversity of exocytosis
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Physiol Rev 92:1915-64. 2012
    ..These distinct initial configurations will help us clarify the common SNARE assembly processes underlying exocytosis and membrane trafficking in eukaryotic cells...
  3. ncbi request reprint Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization
    Haruo Kasai
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Adv Drug Deliv Rev 58:850-77. 2006
    ....
  4. pmc Two components of exocytosis and endocytosis in phaeochromocytoma cells studied using caged Ca2+ compounds
    H Kasai
    Department of Physiology, Faculty of Medicine, University of Tokyo, Japan
    J Physiol 494:53-65. 1996
    ..They seem to couple selectively with specific endocytotic mechanisms. Our data suggest that the two vesicles belong to two distinct secretory pathways...
  5. doi request reprint Learning rules and persistence of dendritic spines
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, and Center for NanoBio Integration, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Eur J Neurosci 32:241-9. 2010
    ..The SGSS mechanism and spine learning rules elucidate the integral nature of synaptic plasticity in neuronal network operations within the actual brain tissue...
  6. doi request reprint Structural dynamics of dendritic spines in memory and cognition
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, and Center for NanoBio Integration, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Trends Neurosci 33:121-9. 2010
    ..Thus, spine dynamics are cellular phenomena with important implications for cognition and memory. Furthermore, impaired spine dynamics can cause psychiatric and neurodevelopmental disorders...
  7. doi request reprint Exocytosis in islet beta-cells
    Haruo Kasai
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, The University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Adv Exp Med Biol 654:305-38. 2010
    ..This chapter provides a concise introduction to the field of optical imaging for those researchers who wish to characterize exocytosis from beta-cells in the islets of Langerhans...
  8. pmc Multiple kinetic components and the Ca2+ requirements of exocytosis
    H Kasai
    Department of Physiology, Faculty of Medicine, University of Tokyo, Japan
    Philos Trans R Soc Lond B Biol Sci 354:331-5. 1999
    ..The effects of local saturation of such indicators are explored by means of a simple theory...
  9. ncbi request reprint Hepatocyte nuclear factor-4alpha is essential for glucose-stimulated insulin secretion by pancreatic beta-cells
    Atsuko Miura
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:5246-57. 2006
    ..Because the K(ATP) channel is important for proper insulin secretion in beta-cells, altered K(ATP) channel activity could be related to the impaired insulin secretion in the betaHNF-4alphaKO mice...
  10. doi request reprint SNARE conformational changes that prepare vesicles for exocytosis
    Noriko Takahashi
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell Metab 12:19-29. 2010
    ..Thus, SNARE proteins exist in multiple stable preparatory configurations, from which Ca(2+) may trigger exocytosis through distinct mechanisms and with distinct kinetics...
  11. pmc In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice
    Jun Noguchi
    Laboratory of Structural Physiology, CDBIM, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, 113 0033, Japan
    J Physiol 589:2447-57. 2011
    ..In vivo 2P uncaging will be a powerful tool to investigate properties of synapses in the neocortex...
  12. ncbi request reprint GABA promotes the competitive selection of dendritic spines by controlling local Ca2+ signaling
    Tatsuya Hayama
    1 Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan 2 CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan 3
    Nat Neurosci 16:1409-16. 2013
    ..Thus, GABAergic inhibition directly suppresses local dendritic Ca(2+) transients and strongly promotes the competitive selection of dendritic spines. ..
  13. doi request reprint Transcranial optogenetic stimulation for functional mapping of the motor cortex
    Riichiro Hira
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    J Neurosci Methods 179:258-63. 2009
    ..This method allows non-invasive mapping of brain circuitry in living animals and could help define the connection between behavior and brain circuitry...
  14. pmc Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells
    Hiroyasu Hatakeyama
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Bunkyo ku, Tokyo, 113 0033 Japan
    J Physiol 582:1087-98. 2007
    ..Thus, Epac and PKA selectively regulate exocytosis of SVs and LVs, respectively, in beta-cells, and Epac can regulate exocytosis more rapidly than PKA...
  15. ncbi request reprint The HNF-1 target collectrin controls insulin exocytosis by SNARE complex formation
    Kenji Fukui
    Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Suita Osaka 565 0871, Japan
    Cell Metab 2:373-84. 2005
    ..Therefore, collectrin is a regulator of SNARE complex function, which thereby controls insulin exocytosis...
  16. doi request reprint Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines
    Jun ichi Tanaka
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Science 319:1683-7. 2008
    ..Thus, this spike-timing protocol is an efficient trigger for BDNF secretion and induces protein synthesis-dependent long-term enlargement at the level of single spines...
  17. ncbi request reprint Two-photon uncaging of gamma-aminobutyric acid in intact brain tissue
    Masanori Matsuzaki
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Nat Chem Biol 6:255-7. 2010
    ..Two-photon uncaging of GABA, the main inhibitory neurotransmitter, should allow detailed studies of receptor function and synaptic integration with subcellular precision...
  18. doi request reprint Three-dimensional mapping of unitary synaptic connections by two-photon macro photolysis of caged glutamate
    Masanori Matsuzaki
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Neurophysiol 99:1535-44. 2008
    ..Thus this technique will be a uniquely powerful tool for quantifying synaptic connectivity and manipulating neural activity at the single-cell level...
  19. ncbi request reprint Exocytic process analyzed with two-photon excitation imaging in endocrine pancreas
    Noriko Takahashi
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Japan
    Endocr J 54:337-46. 2007
    ..Two-photon imaging will be powerful tools to elucidate molecular and cellular mechanisms of exocytosis and related disease, and to develop new therapeutic agencies as well as diagnostic tools...
  20. pmc Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla
    Takuya Kishimoto
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Hongo, Tokyo, Japan
    EMBO J 25:673-82. 2006
    ..We suggest the existence of two molecular configurations for fusion-ready states in Ca2+ -dependent exocytosis...
  21. pmc In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas
    Riichiro Hira
    Division of Brain Circuits, National Institute for Basic Biology Okazaki, Japan CREST, Japan Science and Technology Agency Saitama, Japan Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo Tokyo, Japan
    Front Neural Circuits 7:55. 2013
    ..Our results suggest that neuronal activities in the RFA and the CFA during movements are formed through asymmetric reciprocal connections...
  22. pmc Principles of long-term dynamics of dendritic spines
    Nobuaki Yasumatsu
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    J Neurosci 28:13592-608. 2008
    ..The population dynamics of spines also predict key psychological features of memory...
  23. doi request reprint Simultaneous visualization of multiple neuronal properties with single-cell resolution in the living rodent brain
    Rie Ako
    Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Japan
    Mol Cell Neurosci 48:246-57. 2011
    ....
  24. doi request reprint The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines
    Naoki Honkura
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Neuron 57:719-29. 2008
    ..Ca2+/calmodulin-dependent protein kinase II regulated this confinement. Thus, spines have an elaborate mechanical nature that is regulated by actin fibers...
  25. ncbi request reprint Switch to anaerobic glucose metabolism with NADH accumulation in the beta-cell model of mitochondrial diabetes. Characteristics of betaHC9 cells deficient in mitochondrial DNA transcription
    Mitsuhiko Noda
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Japan
    J Biol Chem 277:41817-26. 2002
    ..This is the first report to demonstrate the connection between metabolic alteration of electron transport system and that of tricarboxylic acid cycle and its impact on insulin secretion...
  26. ncbi request reprint Four novel KVLQT1 and four novel HERG mutations in familial long-QT syndrome
    T Tanaka
    Laboratory of Molecular Medicine, University of Tokyo, Japan
    Circulation 95:565-7. 1997
    ..Three genes responsible for this syndrome (KVLQT1, HERG, and SCN5A) have been identified so far. We investigated mutations of these genes in LQTS families...
  27. pmc Class IA phosphatidylinositol 3-kinase in pancreatic β cells controls insulin secretion by multiple mechanisms
    Kazuma Kaneko
    Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113 8655, Japan
    Cell Metab 12:619-32. 2010
    ..The class IA PI3K pathway in β cells in vivo is important in the regulation of insulin secretion and may be a therapeutic target for type 2 diabetes...
  28. pmc Bernard Katz, quantal transmitter release and the foundations of presynaptic physiology
    George J Augustine
    Division of Biophysics, Center for Disease Biology and Integrative Medicine, University of Tokyo Faculty of Medicine, Tokyo, Japan
    J Physiol 578:623-5. 2007
  29. pmc Simultaneous two-photon activation of presynaptic cells and calcium imaging in postsynaptic dendritic spines
    Masanori Matsuzaki
    Laboratory of Structural Physiology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Neural Syst Circuits 1:2. 2011
    ..abstract:..
  30. ncbi request reprint Correlation of genetic etiology with response to beta-adrenergic blockade among symptomatic patients with familial long-QT syndrome
    T Itoh
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, University of Tokyo, Japan
    J Hum Genet 46:38-40. 2001
    ..0077, Fisher's exact test). This is a good example of the power of genetic diagnosis to direct the selection of appropriate therapy for patients with diseases of heterogeneous genetic etiology...
  31. ncbi request reprint [A New Role of GABA on Synapses]
    Tatsuya Hayama
    Structural Physiology, Graduate School of Medicine, The University of Tokyo
    Brain Nerve 66:987-93. 2014
    ..Our results account well for the in vivo GABA functions on synaptic selection, and may help to develop new therapeutic compounds. ..
  32. ncbi request reprint Hexamminecobalt(III) chloride inhibits glucose-induced insulin secretion at the exocytotic process
    Y Tsubamoto
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
    J Biol Chem 276:2979-85. 2001
    ....
  33. ncbi request reprint Genetic polymorphisms and alternative splicing of the hOGG1 gene, that is involved in the repair of 8-hydroxyguanine in damaged DNA
    T Kohno
    Biology Division, National Cancer Center Research Institute, Tokyo, Japan
    Oncogene 16:3219-25. 1998
    ..These results suggest that the oh8Gua levels are maintained at a steady level, even though multiple hOGG1 proteins are produced due to genetic polymorphisms, mutations and alternative splicing of the hOGG1 gene...
  34. ncbi request reprint Role of NADH shuttle system in glucose-induced activation of mitochondrial metabolism and insulin secretion
    K Eto
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113, Japan Tokyo
    Science 283:981-5. 1999
    ..The NADH shuttle evidently couples glycolysis with activation of mitochondrial energy metabolism to trigger insulin secretion...
  35. ncbi request reprint [Dendritic spine structures and functions]
    Haruo Kasai
    Departement of Cell Physiology, National Institute for Physiological Sciences, Okazaki, 444 8585 Japan
    Nihon Shinkei Seishin Yakurigaku Zasshi 22:159-64. 2002
    ..These observations suggest that a physical basis of the memory in the cerebral cortex resides in the alteration and maintenance of spine structures...
  36. ncbi request reprint Number and density of AMPA receptors in single synapses in immature cerebellum
    Jun ichi Tanaka
    Department of Physiological Sciences, Graduate University for Advanced Studies, Sokendai 444 8787, Japan
    J Neurosci 25:799-807. 2005
    ..Our results indicate that in this developing synapse, the efficacy of transmission is determined by the synaptic area...
  37. ncbi request reprint Spine-neck geometry determines NMDA receptor-dependent Ca2+ signaling in dendrites
    Jun Noguchi
    Department of Cell Physiology, National Institute for Physiological Sciences and Graduate University of Advanced Studies SOKENDAI, Myodaiji, Okazaki 444 8787, Japan
    Neuron 46:609-22. 2005
    ..Spine-neck geometry is thus an important determinant of spine Ca2+ signaling, allowing small spines to be the preferential sites for isolated induction of long-term potentiation...
  38. pmc Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Ting Ting Liu
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:917-29. 2005
    ..Thus, we have, for the first time, directly visualized massive exocytosis of small vesicles in a non-synaptic preparation, and have revealed their fusion-pore mediated exocytosis and endocytosis...
  39. pmc Sequential exocytosis of insulin granules is associated with redistribution of SNAP25
    Noriko Takahashi
    Dept of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    J Cell Biol 165:255-62. 2004
    ..These observations support the hypothesis that SNAP25 is a plasma membrane factor that is responsible for sequential exocytosis...
  40. pmc Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation
    Kazuo Kasai
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    J Clin Invest 115:388-96. 2005
    ....
  41. ncbi request reprint Structural basis of long-term potentiation in single dendritic spines
    Masanori Matsuzaki
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University of Advanced Studies Sokendai, Myodaiji, Okazaki 444 8787, Japan
    Nature 429:761-6. 2004
    ..They further suggest that small spines are preferential sites for long-term potentiation induction, whereas large spines might represent physical traces of long-term memory...
  42. ncbi request reprint Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands
    Akihiro Oshima
    Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies SOKENDAI, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    Cell Calcium 37:349-57. 2005
    ..The delay in sequential compound exocytosis relative to fluid secretion may be important for release of the viscous contents of secretory granules into the nasal cavity...
  43. pmc Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis
    Takuya Kishimoto
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:905-15. 2005
    ....
  44. ncbi request reprint 4-Carboxymethoxy-5,7-dinitroindolinyl-Glu: an improved caged glutamate for expeditious ultraviolet and two-photon photolysis in brain slices
    Graham C R Ellis-Davies
    Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA
    J Neurosci 27:6601-4. 2007
  45. ncbi request reprint Structure-stability-function relationships of dendritic spines
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences and The Graduate University for Advanced Studies SOKENDAI, Okazaki 444 8585, Japan
    Trends Neurosci 26:360-8. 2003
    ..Characterization of supramolecular complexes responsible for synaptic memory and learning is key to the understanding of brain function and disease...
  46. ncbi request reprint [Structure-stability-function relationships of dendritic spines]
    Haruo Kasai
    Tanpakushitsu Kakusan Koso 49:276-81. 2004
  47. pmc Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets
    Hiroyasu Hatakeyama
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    J Physiol 570:271-82. 2006
    ....
  48. ncbi request reprint Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, and the Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    Science 297:1349-52. 2002
    ..When the pore dilated to 12 nanometers, the granules rapidly flattened and discharged their contents. Thus, our methodology reveals fusion pore dynamics in intact tissues at nanometer resolution...
  49. pmc A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to mouse pancreatic islets
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444 8787, Japan
    J Physiol 568:891-903. 2005
    ..It allows quantitative imaging of exocytosis within secretory organs, yielding estimates of vesicle diameter with nanometer resolution...
  50. ncbi request reprint Two-photon excitation imaging of pancreatic islets with various fluorescent probes
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Diabetes 51:S25-8. 2002
    ..In addition, our data suggest that membrane-permeable antagonists may affect only the superficial cell layers of islets, and so their negative effects should be interpreted with caution...
  51. ncbi request reprint Fast and cAMP-sensitive mode of Ca(2+)-dependent exocytosis in pancreatic beta-cells
    Haruo Kasai
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Diabetes 51:S19-24. 2002
    ..In contrast to the fast component of exocytosis, the slow component (mode 2) of this process is independent of cAMP and ATP and can account for the slow component of insulin secretion, which does not require these nucleotides...
  52. ncbi request reprint Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini
    Tomomi Nemoto
    Department of Cell Physiology, National Institute for Physiological Sciences, and Graduate University of Advanced Studies, 5 1 Higashiyama, Myodaiji cho, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 279:37544-50. 2004
    ..Rho-dependent F-actin coating of granule membranes thus stabilizes exocytic structures and is necessary for physiological progression of sequetial compound exocytosis in the exocrine pancreas and for prevention of acute pancreatitis...