Osamu Inoue

Summary

Affiliation: University of Yamanashi
Country: Japan

Publications

  1. ncbi request reprint Redundant mechanism of platelet adhesion to laminin and collagen under flow: involvement of von Willebrand factor and glycoprotein Ib-IX-V
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898 Japan
    J Biol Chem 283:16279-82. 2008
  2. pmc Laminin stimulates spreading of platelets through integrin alpha6beta1-dependent activation of GPVI
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Yamanashi Medical University, 1110 Shimokato Tamaho Nakakoma, Yamanashi 409 3898, Japan
    Blood 107:1405-12. 2006
  3. pmc Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells
    Makoto Osada
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    J Biol Chem 287:22241-52. 2012
  4. ncbi request reprint Docking protein Gab2 positively regulates glycoprotein VI-mediated platelet activation
    Qun Yi
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Tamaho, Nakakoma, Japan
    Biochem Biophys Res Commun 337:446-51. 2005
  5. ncbi request reprint The novel platelet activation receptor CLEC-2
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898, Japan
    Platelets 22:380-4. 2011
  6. pmc Essential in vivo roles of the C-type lectin receptor CLEC-2: embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    J Biol Chem 285:24494-507. 2010
  7. doi request reprint Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan
    FEBS Lett 583:81-7. 2009
  8. doi request reprint Regulation of adaptor protein GIT1 in platelets, leading to the interaction between GIT1 and integrin alpha(IIb)beta3
    Hitomi Sato
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    Biochem Biophys Res Commun 368:157-61. 2008
  9. ncbi request reprint Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898, Japan
    J Biol Chem 282:25993-6001. 2007
  10. ncbi request reprint [A role of the platelet receptor CLEC-2 in lymphangiogenesis and its clinical application]
    Makoto Osada
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo 409 3898, Japan
    Rinsho Byori 61:318-27. 2013

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Redundant mechanism of platelet adhesion to laminin and collagen under flow: involvement of von Willebrand factor and glycoprotein Ib-IX-V
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898 Japan
    J Biol Chem 283:16279-82. 2008
    ..This mechanism is similar to that of collagen. We propose that integrins, GPVI, GPIb-IX-V, and VWF represent a general paradigm for the interaction between platelets and subendothelial matrices...
  2. pmc Laminin stimulates spreading of platelets through integrin alpha6beta1-dependent activation of GPVI
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Yamanashi Medical University, 1110 Shimokato Tamaho Nakakoma, Yamanashi 409 3898, Japan
    Blood 107:1405-12. 2006
    ..We speculate that laminin contributes to platelet spreading in vivo through a direct interaction with GPVI...
  3. pmc Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells
    Makoto Osada
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    J Biol Chem 287:22241-52. 2012
    ..We propose that platelets regulate blood/lymphatic vessel separation by inhibiting the proliferation, migration, and tube formation of LECs, mainly because of the release of BMP-9 upon activation by CLEC-2/podoplanin interaction...
  4. ncbi request reprint Docking protein Gab2 positively regulates glycoprotein VI-mediated platelet activation
    Qun Yi
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Tamaho, Nakakoma, Japan
    Biochem Biophys Res Commun 337:446-51. 2005
    ..These findings provide evidence for a functional role of Gab2 in supporting platelet activation by GPVI...
  5. ncbi request reprint The novel platelet activation receptor CLEC-2
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898, Japan
    Platelets 22:380-4. 2011
    ..In this article and its accompanying poster, we outline the structure, distribution, signal transduction mechanism and functions of CLEC-2...
  6. pmc Essential in vivo roles of the C-type lectin receptor CLEC-2: embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    J Biol Chem 285:24494-507. 2010
    ..We propose that CLEC-2 could be an ideal novel target protein for an anti-platelet drug, which inhibits pathological thrombus formation but not physiological hemostasis...
  7. doi request reprint Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI
    Osamu Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan
    FEBS Lett 583:81-7. 2009
    ..Poly(PHG) is an useful research tool to investigate GPVI-mediated signals and a substitute for collagen in platelet functional assays...
  8. doi request reprint Regulation of adaptor protein GIT1 in platelets, leading to the interaction between GIT1 and integrin alpha(IIb)beta3
    Hitomi Sato
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409 3898, Japan
    Biochem Biophys Res Commun 368:157-61. 2008
    ..These results suggest that tyrosine phosphorylation of GIT1 by Src kinases may regulate cytoskeletal reorganization downstream of alpha(IIb)beta(3) by bringing the Rac GEF betaPIX to the vicinity of the integrin...
  9. ncbi request reprint Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409 3898, Japan
    J Biol Chem 282:25993-6001. 2007
    ..These findings suggest that CLEC-2 is a physiological target protein of podoplanin and imply that it is involved in podoplanin-induced platelet aggregation, tumor metastasis, and other cellular responses related to podoplanin...
  10. ncbi request reprint [A role of the platelet receptor CLEC-2 in lymphangiogenesis and its clinical application]
    Makoto Osada
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo 409 3898, Japan
    Rinsho Byori 61:318-27. 2013
    ..We are now investigating the potential of soluble CLEC-2 as a useful marker for in vivo platelet activation...
  11. ncbi request reprint [Identification of the novel platelet activation receptor CLEC-2 and Its pathological and physiological roles]
    Katsue Suzuki Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo 409 3898, Japan
    Rinsho Byori 58:1193-202. 2010
    ..We propose that CLEC-2 could be an ideal novel target protein for an anti-platelet drug, which inhibits pathological thrombus formation but not physiological hemostasis, as well as a novel target protein for an anti-metastatic drug...
  12. doi request reprint Platelet aggregometry in the presence of PGE(1) provides a reliable method for cilostazol monitoring
    Kaneo Satoh
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Shimokato 1110, Chuo, Yamanashi, Japan
    Thromb Res 130:616-21. 2012
    ..We attempted to establish an assay system for cilostazol monitoring, using platelet aggregation induced by arachidonic acid (AA) in the presence of PGE(1) which upregulates intracellular cyclic AMP...
  13. ncbi request reprint [Recent development in platelet functions: roles beyond thrombosis]
    Yukio Ozaki
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo 409 3815, Japan
    Rinsho Byori 60:52-8. 2012
    ..CLEC-2 interaction with podoplan expressed on lymphatic endothelial cells appears to play an important role in the separation between veins and lymphatic vessels during the stage of fetal development...
  14. pmc Involvement of Src kinases and PLCgamma2 in clot retraction
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, Yamanashi University, 1110 Shimokato, Chuo, Yamanashi, 409 3898, Japan
    Thromb Res 120:251-8. 2007
    ..These results demonstrate a partial role for Src kinase-dependent activation of PLCgamma2 and MLC phosphorylation in mediating clot retraction downstream of integrin alpha(IIb)beta(3)...
  15. doi request reprint Elevated plasma factor VIII enhances venous thrombus formation in rabbits: contribution of factor XI, von Willebrand factor and tissue factor
    Chihiro Sugita
    Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
    Thromb Haemost 110:62-75. 2013
    ..In conclusion, elevated plasma FVIII levels enhance venous thrombus formation and propagation. Excess thrombin generation by FXI and VWF-mediated FVIII recruitment appear to contribute to the growth of FVIII-driven venous thrombus. ..
  16. doi request reprint Causes of thrombocytopenia in chronic hepatitis C viral infection
    Makoto Osada
    Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo, Japan
    Clin Appl Thromb Hemost 18:272-80. 2012
    ..It is positively correlated with vWF and inversely with the platelet count. Our present results imply that vascular endothelial dysfunction is also involved in thrombocytopenia during chronic HCV infection...
  17. ncbi request reprint Grape seed extracts inhibit platelet aggregation by inhibiting protein tyrosine phosphatase
    Joseph Wuxun Jin
    1Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan
    Clin Appl Thromb Hemost 20:278-84. 2014
    ..Moreover, we showed that GSE ingestion inhibited platelet aggregation in mice without enhancing tail bleeding, implying that GSE supplementation might be beneficial to prevention of thrombotic diseases. ..
  18. ncbi request reprint A novel Syk-dependent mechanism of platelet activation by the C-type lectin receptor CLEC-2
    Katsue Suzuki-Inoue
    Department of Clinical and Laboratory Medicine, University of Yamanashi, Shimokato Tamaho Nakakoma, Yamanashi 409 3898, Japan
    Blood 107:542-9. 2006
    ..CLEC-2 is the first C-type lectin receptor to be found on platelets which signals through this novel pathway...
  19. ncbi request reprint Sensitivities of benzodiazepine receptor binding and muscarinic acetylcholine receptor binding for the detection of neural cell death caused by sodium nitroprusside microinjection in rat brain
    Osamu Inoue
    Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Osaka 565 0871, Japan
    Synapse 49:134-41. 2003
    ..These results indicate that mAChR binding may be superior to BZR binding for detecting cell death in brain tissue, in contrast to what was previously thought...
  20. ncbi request reprint Effect of sabcomeline on muscarinic and dopamine receptor binding in intact mouse brain
    Rie Hosoi
    Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Japan
    Ann Nucl Med 17:123-30. 2003
    ..Although the mechanism is still unclear, sabcomeline altered dopamine D2 receptor affinity or availability by modulations via neural networks...
  21. ncbi request reprint Simultaneous determination of serotonin and 5-hydroxyindole-3-acetic acid in human urine by automated precolumn derivatization and semi-microbore column liquid chromatography with fluorescence detection
    Takashi Yoshitake
    Chemicals Evaluation and Research Institute, Japan, 3 822 Ishii machi, Hita, Oita 877 0061, Japan
    Anal Sci 20:1687-90. 2004
    ..7 and 0.5 fmol/20-microl injection). The proposed automatic method permits a highly selective and sensitive determination of 5-HT and 5-HIAA in human urine without any sample purification...
  22. ncbi request reprint Rolipram depresses [(3)H]2-deoxyglucose uptake in mouse brain and heart in vivo
    Megumi Ishikawa
    Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1 7 Yamadaoka, Suita Shi, Osaka, Japan
    Eur J Nucl Med Mol Imaging 29:1212-5. 2002
    ....
  23. ncbi request reprint Molecular analysis of the pathophysiological binding of the platelet aggregation-inducing factor podoplanin to the C-type lectin-like receptor CLEC-2
    Yukinari Kato
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory C 2, 1 1 1 Umezono, Tsukuba, Ibaraki 305 8568, Japan
    Cancer Sci 99:54-61. 2008
    ..Podoplanin and CLEC-2 might represent promising therapeutic targets in cancer metastasis...
  24. ncbi request reprint Association of Fyn and Lyn with the proline-rich domain of glycoprotein VI regulates intracellular signaling
    Katsue Suzuki-Inoue
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom
    J Biol Chem 277:21561-6. 2002
    ..These findings demonstrate that the proline-rich domain of GPVI mediates the association with Fyn/Lyn via their SH3 domain and that this interaction initiates activation signals through GPVI...
  25. ncbi request reprint Vav1, but not Vav2, contributes to platelet aggregation by CRP and thrombin, but neither is required for regulation of phospholipase C
    Andrew C Pearce
    Department of Pharmacology, University of Oxford, United Kingdom
    Blood 100:3561-9. 2002
    ..The present study has provided evidence for a possible role of Vav1 but not Vav2 in the later stages of platelet aggregation...
  26. pmc Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2
    Osamu Inoue
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK
    J Cell Biol 160:769-80. 2003
    ....
  27. ncbi request reprint Murine GPVI stimulates weak integrin activation in PLCgamma2-/- platelets: involvement of PLCgamma1 and PI3-kinase
    Katsue Suzuki-Inoue
    Department of Pharmacology, University of Oxford, United Kingdom
    Blood 102:1367-73. 2003
    ..These results provide strong evidence that engagement of GPVI stimulates limited integrin activation in PLCgamma2-/- platelets via PLCgamma1 and PI3-kinase...
  28. ncbi request reprint Rat-PET study without anesthesia: anesthetics modify the dopamine D1 receptor binding in rat brain
    Sotaro Momosaki
    Department of Brain Science and Molecular Imaging, National Institute for Longevity Sciences, Aichi 474 8522, Japan
    Synapse 54:207-13. 2004
    ..Our results indicate that PET studies in rats should be performed in the conscious state since the anesthetics dramatically modified ligand-receptor bindings, such as DA-D(1) receptor binding, in rat brain...
  29. ncbi request reprint MicroPET detection of enhanced 18F-FDG utilization by PKA inhibitor in awake rat brain
    Rie Hosoi
    Course of Allied Health Sciences, Graduate School of Medicine, Osaka University, 1 7 Yamadaoka, Suita, Osaka 565 0871, Japan
    Brain Res 1039:199-202. 2005
    ..These results strongly demonstrated the value of measuring glucose utilization in the brains of conscious rats...
  30. ncbi request reprint Effects of anesthesia upon 18F-FDG uptake in rhesus monkey brains
    Takashi Itoh
    Center for Information and Sciences, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Ann Nucl Med 19:373-7. 2005
    ..These findings demonstrate that 18F-FDG concentration in the monkey brain is significantly affected by anesthesia. The results also imply the existence of a short-term regulation mechanism for hexokinase activity in intact monkey brain...
  31. ncbi request reprint Binding kinetics of 11C-N-methyl piperidyl benzilate (11C-NMPB) in a rhesus monkey brain using the cerebellum as a reference region
    Takashi Itoh
    Center for Information and Sciences, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Ann Nucl Med 19:499-505. 2005
    ..When 11C-NMPB binding was compared between ketamine-treated and awake animals, a significant increase in 11C-NMPB binding was observed in the striatum but not in other brain regions of ketamine-treated animals...
  32. ncbi request reprint Acute treatment with pentobarbital alters the kinetics of in vivo receptor binding in the mouse brain
    Yojiro Sakiyama
    Division of Clinical Research, National Institute of Radiological Sciences, Inage Ku, Chibashi 263 8555, Japan
    Nucl Med Biol 33:535-41. 2006
    ..These results suggest that apparent increases in binding at 20 min after tracer injection were due to the decrease in the association and dissociation rates of binding in vivo...
  33. ncbi request reprint Lack of cyclic AMP-specific phosphodiesterase 4 activation during naloxone-precipitated morphine withdrawal in rats
    Megumi Kimura
    Department of Drug Safety Evaluation, Developmental Research Laboratories, Shionogi and Co Ltd, Toyonaka, Osaka, Japan
    Neurosci Lett 404:107-11. 2006
    ..These results suggest that the lack of PDE4 activation leading to remarkable elevation of cyclic AMP is involved in naloxone-precipitated morphine withdrawal symptoms...