N Hirokawa

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi Kinesin and dynein superfamily proteins in organelle transport and cell division
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Curr Opin Cell Biol 10:60-73. 1998
  2. ncbi Kinesin transports RNA: isolation and characterization of an RNA-transporting granule
    Yoshimitsu Kanai
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Neuron 43:513-25. 2004
  3. ncbi Nodal flow and the generation of left-right asymmetry
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 125:33-45. 2006
  4. ncbi KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons
    Laurent Guillaud
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    J Neurosci 23:131-40. 2003
  5. ncbi Molecular motors in neuronal development, intracellular transport and diseases
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Curr Opin Neurobiol 14:564-73. 2004
  6. ncbi Cilia, KIF3 molecular motor and nodal flow
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Curr Opin Cell Biol 24:31-9. 2012
  7. ncbi mRNA transport in dendrites: RNA granules, motors, and tracks
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Neurosci 26:7139-42. 2006
  8. ncbi Intracellular transport and kinesin superfamily proteins, KIFs: structure, function, and dynamics
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Physiol Rev 88:1089-118. 2008
  9. ncbi Influencing science policy in Japan
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Nat Rev Mol Cell Biol 2:933-5. 2001
  10. ncbi Stirring up development with the heterotrimeric kinesin KIF3
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1 Bunkyo ku, Tokyo, Japan
    Traffic 1:29-34. 2000

Collaborators

Detail Information

Publications92

  1. ncbi Kinesin and dynein superfamily proteins in organelle transport and cell division
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Curr Opin Cell Biol 10:60-73. 1998
    ..Sophisticated biophysical and structural analyses of monomeric and dimeric motor proteins have contributed to elucidating the mechanisms behind motor protein motility and polarity...
  2. ncbi Kinesin transports RNA: isolation and characterization of an RNA-transporting granule
    Yoshimitsu Kanai
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Neuron 43:513-25. 2004
    ..Thus, kinesin transports RNA via this granule in dendrites coordinately with opposite motors, such as dynein...
  3. ncbi Nodal flow and the generation of left-right asymmetry
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 125:33-45. 2006
    ....
  4. ncbi KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons
    Laurent Guillaud
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    J Neurosci 23:131-40. 2003
    ..Taken together, these results illustrate the complex mechanisms underlying the active transport and regulation of NR2B by the molecular motor KIF17 in living hippocampal neurons...
  5. ncbi Molecular motors in neuronal development, intracellular transport and diseases
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Curr Opin Neurobiol 14:564-73. 2004
    ..In dendrites, the transport of NMDA and AMPA receptors is mediated by KIFs, and the motor has been shown to play a significant part in establishing learning and memory...
  6. ncbi Cilia, KIF3 molecular motor and nodal flow
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Curr Opin Cell Biol 24:31-9. 2012
    ....
  7. ncbi mRNA transport in dendrites: RNA granules, motors, and tracks
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Neurosci 26:7139-42. 2006
    ..This transport is fundamental to local protein synthesis and to the regulation of neuronal function...
  8. ncbi Intracellular transport and kinesin superfamily proteins, KIFs: structure, function, and dynamics
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Physiol Rev 88:1089-118. 2008
    ....
  9. ncbi Influencing science policy in Japan
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Nat Rev Mol Cell Biol 2:933-5. 2001
    ..Council for Science and Technology Policy (CSTP) has been given great powers - but how, in particular, is the funding policy being addressed by the new administrative structure, and how can scientists influence science policy in Japan?..
  10. ncbi Stirring up development with the heterotrimeric kinesin KIF3
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1 Bunkyo ku, Tokyo, Japan
    Traffic 1:29-34. 2000
    ....
  11. ncbi Left-right determination: involvement of molecular motor KIF3, cilia, and nodal flow
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1 Hongo, Tokyo, 113 0033 Japan
    Cold Spring Harb Perspect Biol 1:a000802. 2009
    ....
  12. ncbi Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease
    Nobutaka Hirokawa
    Departments of Cell Biology and Anatomy and Molecular Structure and Dynamics, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Neuron 68:610-38. 2010
    ..Finally, it is also evident that molecular motors are critically involved in neuronal disease pathogenesis. Thus, molecular motor research is becoming an exciting frontier of neuroscience...
  13. ncbi The mechanisms of fast and slow transport in neurons: identification and characterization of the new kinesin superfamily motors
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan 113
    Curr Opin Neurobiol 7:605-14. 1997
    ..The results of these studies strongly support the subunit transport theory...
  14. ncbi Kinesin and dynein superfamily proteins and the mechanism of organelle transport
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Tokyo, Japan
    Science 279:519-26. 1998
    ..This review focuses on the molecular mechanism of organelle transport in cells and describes kinesin and dynein superfamily proteins...
  15. ncbi Kinesin superfamily motor proteins and intracellular transport
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, University of Tokyo, Japan
    Nat Rev Mol Cell Biol 10:682-96. 2009
    ..These findings open exciting new areas of kinesin research...
  16. ncbi From electron microscopy to molecular cell biology, molecular genetics and structural biology: intracellular transport and kinesin superfamily proteins, KIFs: genes, structure, dynamics and functions
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, 7 3 1, Bunkyo ku, Tokyo, Japan 113 0033
    J Electron Microsc (Tokyo) 60:S63-92. 2011
    ..Thus, this type of studies could be a good example of really integrative multidisciplinary life science in the twenty-first century...
  17. ncbi Molecular motors and mechanisms of directional transport in neurons
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Rev Neurosci 6:201-14. 2005
    ..Here we discuss the molecular mechanisms of directional axonal and dendritic transport with specific emphasis on the role of motor proteins and their mechanisms of cargo recognition...
  18. ncbi The mechanisms of kinesin motor motility: lessons from the monomeric motor KIF1A
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, Bunkyo ku, Tokyo, Japan
    Nat Rev Mol Cell Biol 10:877-84. 2009
    ....
  19. ncbi Biochemical and molecular characterization of diseases linked to motor proteins
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, 7 3 1, Bunkyo ku, 113 0033, Tokyo, Japan
    Trends Biochem Sci 28:558-65. 2003
    ..Here, we discuss the relationship of these motor proteins and how they contribute to disease in molecular terms...
  20. ncbi Charcot-Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta
    C Zhao
    Department of Cell Biology and Anatomy, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Cell 105:587-97. 2001
    ..We show that CMT2A patients contain a loss-of-function mutation in the motor domain of the KIF1B gene. This is clear indication that defects in axonal transport due to a mutated motor protein can underlie human peripheral neuropathy...
  21. ncbi KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles
    N Saito
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Hongo, Japan
    Neuron 18:425-38. 1997
    ....
  22. ncbi A novel motor, KIF13A, transports mannose-6-phosphate receptor to plasma membrane through direct interaction with AP-1 complex
    T Nakagawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 103:569-81. 2000
    ..Functional blockade of KIF13A reduced cell surface expression of the M6PR. Thus, KIF13A transports M6PR-containing vesicles and targets the M6PR from TGN to the plasma membrane via direct interaction with the AP-1 adaptor complex...
  23. ncbi A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally
    Y Sekine
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    J Cell Biol 127:187-201. 1994
    ..Hence we conclude that KIF4 is a novel microtubule-associated anterograde motor protein for membranous organelles, the expression of which is regulated developmentally...
  24. ncbi Defect in synaptic vesicle precursor transport and neuronal cell death in KIF1A motor protein-deficient mice
    Y Yonekawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo 113, Japan
    J Cell Biol 141:431-41. 1998
    ..Thus, our results demonstrate that KIF1A transports a synaptic vesicle precursor and that KIF1A-mediated axonal transport plays a critical role in viability, maintenance, and function of neurons, particularly mature neurons...
  25. ncbi Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization
    J Teng
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Cell Biol 155:65-76. 2001
    ....
  26. ncbi Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B
    H Yamazaki
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 93:8443-8. 1996
    ....
  27. ncbi KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria
    M Nangaku
    Department of Anatomy and Cell Biology School of Medicine, University of Tokyo, Japan
    Cell 79:1209-20. 1994
    ..These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria...
  28. ncbi Defects in axonal elongation and neuronal migration in mice with disrupted tau and map1b genes
    Y Takei
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Cell Biol 150:989-1000. 2000
    ..These findings indicate the cooperative functions of tau and MAP1B in vivo in axonal elongation and neuronal migration as regulators of microtubule organization...
  29. ncbi KIF5C, a novel neuronal kinesin enriched in motor neurons
    Y Kanai
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Neurosci 20:6374-84. 2000
    ....
  30. ncbi Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria
    Y Tanaka
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Japan
    Cell 93:1147-58. 1998
    ..These data collectively indicate that kinesin is essential for mitochondrial and lysosomal dispersion rather than for the Golgi-to-ER traffic in these cells...
  31. ncbi KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes
    Y Noda
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    J Cell Biol 155:77-88. 2001
    ..Absence of cytoplasmic dynein on these annexin XIIIb-associated vesicles and distinct distribution of the two motors on the EM level verified the existence of KIFC3-driven transport in epithelial cells...
  32. ncbi KIF2 is a new microtubule-based anterograde motor that transports membranous organelles distinct from those carried by kinesin heavy chain or KIF3A/B
    Y Noda
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    J Cell Biol 129:157-67. 1995
    ..Our results strongly suggest that KIF2 functions as a new anterograde motor, being specialized for a particular group of membranous organelles involved in fast axonal transport...
  33. ncbi The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors
    Y Okada
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    Cell 81:769-80. 1995
    ..Thus, we suggest that the membrane proteins are sorted into different classes of transport organelles in the cell body and are transported by their specific motor proteins through the axon...
  34. ncbi Golgi vesiculation and lysosome dispersion in cells lacking cytoplasmic dynein
    A Harada
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, 113, Japan
    J Cell Biol 141:51-9. 1998
    ..Moreover, cDHC is required for cell proliferation and proper distribution of endosomes and lysosomes. However, molecules other than cDHC might mediate attachment of the Golgi complex and endosomes/lysosomes to microtubules...
  35. ncbi Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis
    S Takeda
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1, Hongo, Bunkyo ku, Tokyo 113 0033 Japan
    J Cell Biol 145:825-36. 1999
    ..These results suggest that KIF3A might be involved in mesodermal patterning and in turn neurogenesis...
  36. ncbi Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein
    S Nonaka
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Cell 95:829-37. 1998
    ....
  37. ncbi 15 A resolution model of the monomeric kinesin motor, KIF1A
    M Kikkawa
    Department of Cell Biology, Graduate School of Medicine, University of Tokyo, Japan
    Cell 100:241-52. 2000
    ..The site-specific cross-linking further confirmed this model...
  38. ncbi Abnormal nodal flow precedes situs inversus in iv and inv mice
    Y Okada
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Japan
    Mol Cell 4:459-68. 1999
    ..These results consistently support our hypothesis that the nodal flow produces the gradient of putative morphogen and triggers the first L-R determination event...
  39. ncbi Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport
    M Setou
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Science 288:1796-802. 2000
    ..This interaction, specific for a neurotransmitter receptor critically important for plasticity in the postsynaptic terminal, may be a regulatory point for synaptic plasticity and neuronal morphogenesis...
  40. ncbi Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system
    Y Takei
    Department of Anatomy and Cell Biology, University of Tokyo, Japan
    J Cell Biol 131:1789-800. 1995
    ..These results suggest significant contribution of synapsin I to the formation and maintenance of the presynaptic structure...
  41. ncbi KIF3A is a new microtubule-based anterograde motor in the nerve axon
    S Kondo
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    J Cell Biol 125:1095-107. 1994
    ..These findings strongly suggest that KIF3A is a new motor protein for the anterograde fast axonal transport...
  42. ncbi Switch-based mechanism of kinesin motors
    M Kikkawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-0033, Japan
    Nature 411:439-45. 2001
    ..Docking of the ADP-bound and ATP-like crystallographic models of KIF1A into the corresponding cryo-electron microscopy maps suggests a rationale for the plus-end directional bias associated with the kinesin catalytic core...
  43. ncbi KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport
    H Yamazaki
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    J Cell Biol 130:1387-99. 1995
    ....
  44. ncbi All kinesin superfamily protein, KIF, genes in mouse and human
    H Miki
    Department of Cell Biology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan
    Proc Natl Acad Sci U S A 98:7004-11. 2001
    ..This report will set the foundation of KIF and intracellular transport research...
  45. ncbi The primary structure of rat brain (cytoplasmic) dynein heavy chain, a cytoplasmic motor enzyme
    Z Zhang
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 90:7928-32. 1993
    ....
  46. ncbi A novel member of the dynamin family of GTP-binding proteins is expressed specifically in the testis
    T Nakata
    Department of Anatomy and Cell Biology, University of Tokyo, School of Medicine, Japan
    J Cell Sci 105:1-5. 1993
    ..Our data imply that a number of dynamin family proteins, which are products of distinct genes, may play different roles specific to each cell type in the same rat...
  47. ncbi Identification and molecular evolution of new dynein-like protein sequences in rat brain
    Y Tanaka
    Department of Anatomy and Cell Biology, Faculty of Medicine, University of Tokyo, Japan
    J Cell Sci 108:1883-93. 1995
    ..Such evolutionary topology builds up an inceptive hypothesis that there are at least two non-axonemal dynein heavy chains in mammals...
  48. ncbi Impairment of inhibitory synaptic transmission in mice lacking synapsin I
    S Terada
    Department of Cellular Neurobiology, University of Tokyo, Graduate School of Medicine, Tokyo 113 0033, Japan
    J Cell Biol 145:1039-48. 1999
    ..This may explain, at least in part, the epileptic seizures occurring in the synapsin I mutant mice...
  49. ncbi Organization of cortical cytoskeleton of cultured chromaffin cells and involvement in secretion as revealed by quick-freeze, deep-etching, and double-label immunoelectron microscopy
    T Nakata
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    J Neurosci 12:2186-97. 1992
    ....
  50. ncbi A processive single-headed motor: kinesin superfamily protein KIF1A
    Y Okada
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Science 283:1152-7. 1999
    ..The movement along the microtubules was stochastic and fitted a biased Brownian-movement model...
  51. ncbi Altered microtubule organization in small-calibre axons of mice lacking tau protein
    A Harada
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    Nature 369:488-91. 1994
    ..Our results argue against the suggested role of tau in axonal elongation but confirm that it is crucial in the stabilization and organization of axonal microtubules in a certain type of axon...
  52. ncbi Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B (MAP1B) gene
    Y Takei
    Department of Cell Biology and Anatomy, Faculty of Medicine, University of Tokyo, Tokyo, 113, Japan
    J Cell Biol 137:1615-26. 1997
    ....
  53. ncbi Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head
    Takao Nakata
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7-3-1, Hongo, Tokyo, Japan 113-0033
    J Cell Biol 162:1045-55. 2003
    ..These findings revealed unique features of the microtubule cytoskeletons in the initial segment, and suggested that they provide directional information for polarized axonal transport...
  54. ncbi MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction
    Akihiro Harada
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Japan
    J Cell Biol 158:541-9. 2002
    ..Therefore, MAP2 is an anchoring protein of PKA in dendrites, whose loss leads to reduced amount of dendritic and total PKA and reduced activation of CREB...
  55. ncbi Kinesin family in murine central nervous system
    H Aizawa
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    J Cell Biol 119:1287-96. 1992
    ..All these members of the kinesin family are expressed in the same type of neurons, and thus each one of them may transport its specific organelle in the murine central nervous system...
  56. ncbi Moving on to the cargo problem of microtubule-dependent motors in neurons
    S Terada
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    Curr Opin Neurobiol 10:566-73. 2000
    ..On the basis of new biophysical and cell-biological evidence, the controversy over the movement of cytoplasmic cargoes has entered a new phase...
  57. ncbi Muscle weakness, hyperactivity, and impairment in fear conditioning in tau-deficient mice
    S Ikegami
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Neurosci Lett 279:129-32. 2000
    ..These phenotypes parallel some signs and symptoms of FTDP-17 patients. Our results show that the loss of tau protein may itself lead to some of the neurological characteristics observed in FTDP-17 patients...
  58. ncbi Kinesin superfamily protein 2A (KIF2A) functions in suppression of collateral branch extension
    Noriko Homma
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo Bunkyo ku, Tokyo 113 0033, Japan
    Cell 114:229-39. 2003
    ..Based on these results, we propose that KIF2A regulates microtubule dynamics at the growth cone edge by depolymerizing microtubules and that it plays an important role in the suppression of collateral branch extension...
  59. ncbi Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA
    Y Kanai
    Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan
    J Cell Biol 109:1173-84. 1989
    ..Thus, we suppose that tau proteins promote polymerization of tubulin, form bundles of microtubules in vivo, and play important roles in growing and maintaining nerve cell processes...
  60. ncbi FGF-induced vesicular release of Sonic hedgehog and retinoic acid in leftward nodal flow is critical for left-right determination
    Yosuke Tanaka
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113 0033, Japan
    Nature 435:172-7. 2005
    ..Thus, we propose that NVP flow is a new mode of extracellular transport that forms a left-right gradient of morphogens...
  61. ncbi Kinesin superfamily proteins and their various functions and dynamics
    Nobutaka Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Exp Cell Res 301:50-9. 2004
    ..These multiple approaches in analyzing KIF functions will illuminate many basic mechanisms underlying intracellular events and will be a very promising and fruitful area for future studies...
  62. ncbi The KIF3 motor transports N-cadherin and organizes the developing neuroepithelium
    Junlin Teng
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Cell Biol 7:474-82. 2005
    ..Furthermore, in KAP3-deficient cells, the subcellular localization of N-cadherin was disrupted. Taken together, these results suggest a potential tumour-suppressing activity for this molecular motor...
  63. ncbi Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites
    Mitsutoshi Setou
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 417:83-7. 2002
    ..These results indicate that directly binding proteins can determine the traffic direction of a motor protein...
  64. ncbi A common mechanism for microtubule destabilizers-M type kinesins stabilize curling of the protofilament using the class-specific neck and loops
    Tadayuki Ogawa
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
    Cell 116:591-602. 2004
    ..The open conformation of the switch I loop could be reversed by the shift of the microtubule binding L8 loop, suggesting its role as the sensor to trigger ATP hydrolysis. Mutational analysis supports these structural implications...
  65. ncbi Disruption of KIF17-Mint1 interaction by CaMKII-dependent phosphorylation: a molecular model of kinesin-cargo release
    Laurent Guillaud
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Cell Biol 10:19-29. 2008
    ....
  66. ncbi Association of Ash/Grb-2 with dynamin through the Src homology 3 domain
    H Miki
    Department of Molecular Oncology, University of Tokyo, Japan
    J Biol Chem 269:5489-92. 1994
    ..These data show that Ash SH3 domains bind to the proline-rich region of dynamin. Considering the function of dynamin in membrane trafficking, Ash may regulate endocytosis in addition to Ras activation...
  67. ncbi Gene targeting studies begin to reveal the function of neurofilament proteins
    N Hirokawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, 113-0033 Japan
    J Cell Biol 143:1-4. 1998
  68. ncbi Structural model for strain-dependent microtubule activation of Mg-ADP release from kinesin
    Ryo Nitta
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Nat Struct Mol Biol 15:1067-75. 2008
    ..We further propose that this set of events is linked to a strain-dependent docking of the neck linker to the motor core, which produces a two-step power stroke...
  69. ncbi Mouse models of Charcot-Marie-Tooth disease
    Yosuke Tanaka
    Dept of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, 113-0033, Tokyo, Japan
    Trends Genet 18:S39-44. 2002
    ..Such mice are good disease models for cell biological and therapeutic studies...
  70. ncbi Molecular motor KIF1C is not essential for mouse survival and motor-dependent retrograde Golgi apparatus-to-endoplasmic reticulum transport
    Kazuo Nakajima
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Mol Cell Biol 22:866-73. 2002
    ..This retrograde membrane transport was further confirmed to be normal by time-lapse analysis. Consequently, KIF1C is dispensable for the motor-dependent retrograde transport from the Golgi apparatus to the ER...
  71. ncbi KIF1A alternately uses two loops to bind microtubules
    Ryo Nitta
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Science 305:678-83. 2004
    ..ADP-vanadate displays an intermediate structure in which a conformational change in two switch regions causes both loops to be raised from the microtubule, thus actively detaching kinesin...
  72. ncbi Role of KIFC3 motor protein in Golgi positioning and integration
    Ying Xu
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Cell Biol 158:293-303. 2002
    ..These results collectively demonstrate that KIFC3 plays a complementary role in Golgi positioning and integration with cytoplasmic dynein...
  73. ncbi Mechanism of nodal flow: a conserved symmetry breaking event in left-right axis determination
    Yasushi Okada
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Cell 121:633-44. 2005
    ..Finally, we experimentally and theoretically characterize under which conditions a morphogen gradient can arise from the flow...
  74. ncbi KIF1Bbeta- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through DENN/MADD
    Shinsuke Niwa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Cell Biol 10:1269-79. 2008
    ..GTP-Rab3 was more effectively transported than GDP-Rab3, suggesting that the nucleotide state of Rab3 regulates axonal transport of Rab3-carrying vesicles through preferential interaction with DENN/MADD...
  75. ncbi Kinesin-1/Hsc70-dependent mechanism of slow axonal transport and its relation to fast axonal transport
    Sumio Terada
    Department of Cell Biology and Anatomy, University of Tokyo Graduate School of Medicine, Bunkyo ku, Tokyo, Japan
    EMBO J 29:843-54. 2010
    ..These findings provide a basis for the regulatory mechanism of intracellular transport and its intriguing implication in neuronal dysfunction...
  76. ncbi Neuronal polarity and the kinesin superfamily proteins
    Takao Nakata
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Tokyo, Japan, 113 0033
    Sci STKE 2007:pe6. 2007
    ..In particular, KIF13B transports phosphatidylinositol (3,4,5)-trisphosphate, which, based on current hypotheses, is one of the most upstream molecules in the intracellular signaling cascades involved in axonal formation...
  77. ncbi Processivity of the single-headed kinesin KIF1A through biased binding to tubulin
    Yasushi Okada
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Nature 424:574-7. 2003
    ..We propose a simple physical formulation to explain the movement of KIF1A...
  78. ncbi Analysis of the kinesin superfamily: insights into structure and function
    Harukata Miki
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyoku, Tokyo, Japan
    Trends Cell Biol 15:467-76. 2005
    ..This comprehensive classification builds on the recently proposed standardized nomenclature for kinesins and allows systematic analysis of the structural and functional relationships within the kinesin superfamily...
  79. ncbi KIF4 motor regulates activity-dependent neuronal survival by suppressing PARP-1 enzymatic activity
    Ryosuke Midorikawa
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Tokyo 113 0033, Japan
    Cell 125:371-83. 2006
    ..We suggested that KIF4 controls the activity-dependent survival of postmitotic neurons by regulating PARP-1 activity in brain development...
  80. ncbi Observation of nodal cilia movement and measurement of nodal flow
    Yasushi Okada
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    Methods Cell Biol 91:265-85. 2009
    ..This chapter reviews techniques for the observation of nodal cilia movement and nodal flow in living vertebrate embryos...
  81. ncbi KIF26A is an unconventional kinesin and regulates GDNF-Ret signaling in enteric neuronal development
    Ruyun Zhou
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell 139:802-13. 2009
    ..We therefore propose that the unconventional kinesin KIF26A plays a key role in enteric nervous system development by repressing a cell growth signaling pathway...
  82. ncbi Kinesin superfamily proteins (KIFs) in the mouse transcriptome
    Harukata Miki
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Genome Res 13:1455-65. 2003
    ..This report provides a foundation for future research of the intracellular transport along microtubules and proves the significance of intracellular transport protein transcripts as part of the transcriptome...
  83. ncbi Gem GTPase and tau: morphological changes induced by gem GTPase in cho cells are antagonized by tau
    Fumitaka Oyama
    Department of Neuropathology, Faculty of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 279:27272-7. 2004
    ..Taken together, the present results indicate that the level of Gem GTPase and its cell elongation activity are modulated by tau and suggest that tau may be involved in a Gem GTPase-mediated signal transduction pathway...
  84. ncbi Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice
    Richard Wing Chuen Wong
    Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 99:14500-5. 2002
    ..Our results suggest that the motor protein KIF17 contributes to neuronal events required for learning and memory by trafficking fundamental N-methyl-d-aspartate-type glutamate receptors...
  85. ncbi High-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations
    Masahide Kikkawa
    Department of Cell Biology, Southwestern Medical Center, University of Texas, Dallas, TX, USA
    EMBO J 25:4187-94. 2006
    ..These results suggest a structural model of biased diffusion movement of monomeric kinesin motor...
  86. ncbi Nodal cilia dynamics and the specification of the left/right axis in early vertebrate embryo development
    Javier Buceta
    Centre de Recerca en Química Teòrica CeRQT, Parc Cientific de Barcelona, Campus Diagonal Universitat de Barcelona, Edifici Modular, Barcelona, Spain
    Biophys J 89:2199-209. 2005
    ..In addition, we discuss how the combination of internal and external mechanisms might cause the two-phase beating cilia dynamics...
  87. ncbi Three-dimensional structures of the flagellar dynein-microtubule complex by cryoelectron microscopy
    Toshiyuki Oda
    Department of Cell Biology, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 177:243-52. 2007
    ..7 nm toward the B tubule and inclined 44 degrees inwards. These observations suggest that there is a mechanism that converts head movement into the axonemal sliding motion...
  88. ncbi Dynein and kinesin share an overlapping microtubule-binding site
    Naoko Mizuno
    Department of Cell Biology, Southwestern Medical Center, University of Texas, Dallas, TX 75390 9039, USA
    EMBO J 23:2459-67. 2004
    ..Our results demonstrate that dynein and kinesin share an overlapping microtubule-binding site, and imply that binding at this site has an essential role for these motor proteins...
  89. ncbi Analysis of the mouse transcriptome for genes involved in the function of the nervous system
    Stefano Gustincich
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 13:1395-401. 2003
    ..A list of genes containing CAG stretches in the coding region represents a first step in the potential identification of candidates for hereditary neurological disorders...
  90. ncbi CREM-dependent transcription in male germ cells controlled by a kinesin
    Betina Macho
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, B P 10142, 67404 Illkirch, Strasbourg, France
    Science 298:2388-90. 2002
    ..Thus, a kinesin directly controls the activity of a transcriptional coactivator by a tight regulation of its intracellular localization...
  91. ncbi The functional cooperation of MAP1A heavy chain and light chain 2 in the binding of microtubules
    Chung Liang Chien
    Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, No 1, Section 1, Jen Ai Road, Taipei, 100, Taiwan
    Exp Cell Res 308:446-58. 2005
    ..From our analysis of MAP1A functional domains, we suggest that interactions between MAP1A heavy chain and LC2 are critical for the binding of microtubules...
  92. ncbi A standardized kinesin nomenclature
    Carolyn J Lawrence
    Department of Plant Biology, The University of Georgia, Athens, GA 30602, USA
    J Cell Biol 167:19-22. 2004
    ..The scheme unifies all previous phylogenies and nomenclature proposals, while allowing individual sequence names to remain the same, and for expansion to occur as new sequences are discovered...