Ryouichi Fukuda

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint [Intracellular membrane fusion and SNARE hypothesis]
    Ryouichi Fukuda
    Tanpakushitsu Kakusan Koso 47:761-9. 2002
  2. ncbi request reprint Metabolism of hydrophobic carbon sources and regulation of it in n-alkane-assimilating yeast Yarrowia lipolytica
    Ryouichi Fukuda
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 77:1149-54. 2013
  3. pmc Yas3p, an Opi1 family transcription factor, regulates cytochrome P450 expression in response to n-alkanes in Yarrowia lipolytica
    Kiyoshi Hirakawa
    Department of Biotechnology, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 284:7126-37. 2009
  4. pmc Basic helix-loop-helix transcription factor heterocomplex of Yas1p and Yas2p regulates cytochrome P450 expression in response to alkanes in the yeast Yarrowia lipolytica
    Setsu Endoh-Yamagami
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo, 113 8657, Japan
    Eukaryot Cell 6:734-43. 2007
  5. doi request reprint Involvement of Golgi-associated retrograde protein complex in the recycling of the putative Dnf aminophospholipid flippases in yeast
    Keiko Takagi
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 417:490-4. 2012
  6. ncbi request reprint Phosphatidic acid and phosphoinositides facilitate liposome association of Yas3p and potentiate derepression of ARE1 (alkane-responsive element one)-mediated transcription control
    Satoshi Kobayashi
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Fungal Genet Biol 61:100-10. 2013
  7. doi request reprint Transcriptional repression by glycerol of genes involved in the assimilation of n-alkanes and fatty acids in yeast Yarrowia lipolytica
    Katsuki Mori
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    FEMS Yeast Res 13:233-40. 2013
  8. doi request reprint Incorporation and remodeling of phosphatidylethanolamine containing short acyl residues in yeast
    Lan Deng
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochim Biophys Acta 1801:635-45. 2010
  9. ncbi request reprint Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae
    Lan Deng
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 72:2362-8. 2008
  10. ncbi request reprint Acyl-chain remodeling of dioctanoyl-phosphatidylcholine in Saccharomyces cerevisiae mutant defective in de novo and salvage phosphatidylcholine synthesis
    Hideyuki Kishino
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 445:289-93. 2014

Collaborators

  • Hiroyuki Horiuchi
  • Masaru Tanokura
  • Koji Nagata
  • Ryo Taguchi
  • Maki Kawai-Yamada
  • Etsuro Yoshimura
  • Shigeaki Kato
  • Akinori Ohta
  • Satoshi Kobayashi
  • Lan Deng
  • Yusuke Ono
  • Kiyoshi Hirakawa
  • Kunihiko Iwamoto
  • Toru Kakihara
  • Keiko Takagi
  • Jun Ohtsuka
  • Setsu Endoh-Yamagami
  • Hideyuki Kishino
  • Siqi Tian
  • Yoshiyuki Sagehashi
  • Katsuki Mori
  • Ryo Iwama
  • Hiroshi Takai
  • Hiroki Eguchi
  • Napapol Poopanitpan
  • Kunihiko Tanaka
  • Shingo Kobayashi
  • Reiko Oshima
  • Haeng Seog Lee
  • Daisuke Morioka
  • Masayuki Kakimoto
  • Setsu Yamagami
  • Shipeng Wang
  • Koichi Narita
  • Takuro Inoue
  • Jumpei Nagasawa
  • Yasuhisa Ishikawa
  • Shinya Nagasawa
  • Yusuke Nakatani
  • Hiroki Nakanishi
  • Hidenori Watanabe
  • Yuri Ihara-Ohori
  • Keiko Yoshinaga
  • Hirofumi Uchimiya
  • Woo Cheol Lee
  • Shin ichi Sasagawa
  • Atsushi Kobayashi
  • Yasuke Ono
  • Toshihide Kobayashi
  • Masato Umeda

Detail Information

Publications26

  1. ncbi request reprint [Intracellular membrane fusion and SNARE hypothesis]
    Ryouichi Fukuda
    Tanpakushitsu Kakusan Koso 47:761-9. 2002
  2. ncbi request reprint Metabolism of hydrophobic carbon sources and regulation of it in n-alkane-assimilating yeast Yarrowia lipolytica
    Ryouichi Fukuda
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 77:1149-54. 2013
    ..Transcription of the genes involved in fatty acid utilization and peroxisome biogenesis is controlled by Ctf1-family Zn2Cys6 type transcription factor Por1p in response to fatty acids in Y. lipolytica. ..
  3. pmc Yas3p, an Opi1 family transcription factor, regulates cytochrome P450 expression in response to n-alkanes in Yarrowia lipolytica
    Kiyoshi Hirakawa
    Department of Biotechnology, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    J Biol Chem 284:7126-37. 2009
    ..In contrast, YAS3 deletion did not affect the mRNA level of an INO1 ortholog in Y. lipolytica, indicating functional diversity of Opi1 family transcription factors...
  4. pmc Basic helix-loop-helix transcription factor heterocomplex of Yas1p and Yas2p regulates cytochrome P450 expression in response to alkanes in the yeast Yarrowia lipolytica
    Setsu Endoh-Yamagami
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo, 113 8657, Japan
    Eukaryot Cell 6:734-43. 2007
    ..These findings allow us to postulate a model in which bHLH transcription factors Yas1p and Yas2p form a heterocomplex and mediate the transcription induction in response to alkanes...
  5. doi request reprint Involvement of Golgi-associated retrograde protein complex in the recycling of the putative Dnf aminophospholipid flippases in yeast
    Keiko Takagi
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 417:490-4. 2012
    ..Our results suggest that the GARP complex is involved in the recycling of Dnf flippases...
  6. ncbi request reprint Phosphatidic acid and phosphoinositides facilitate liposome association of Yas3p and potentiate derepression of ARE1 (alkane-responsive element one)-mediated transcription control
    Satoshi Kobayashi
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Fungal Genet Biol 61:100-10. 2013
    ..Truncation mutation analyses for Yas3p revealed two regions that bound to PA and PIPs. These results suggest that the interaction with acidic phospholipids is important for the n-alkane-induced association of Yas3p with the ER membrane. ..
  7. doi request reprint Transcriptional repression by glycerol of genes involved in the assimilation of n-alkanes and fatty acids in yeast Yarrowia lipolytica
    Katsuki Mori
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    FEMS Yeast Res 13:233-40. 2013
    ..lipolytica. Repression of ALK1 transcription by glycerol was not observed in the deletion mutant of GUT1 encoding glycerol kinase, implying that the phosphorylation of glycerol is required for the glycerol repression...
  8. doi request reprint Incorporation and remodeling of phosphatidylethanolamine containing short acyl residues in yeast
    Lan Deng
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochim Biophys Acta 1801:635-45. 2010
    ..Our results also suggest the presence of a mechanism to maintain the physiological length of PE acyl residues in yeast...
  9. ncbi request reprint Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae
    Lan Deng
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 72:2362-8. 2008
    ..In addition, sporulation was also impaired in cells with a lowered sterol content, raising the possibility that sterol content is also important for spore formation...
  10. ncbi request reprint Acyl-chain remodeling of dioctanoyl-phosphatidylcholine in Saccharomyces cerevisiae mutant defective in de novo and salvage phosphatidylcholine synthesis
    Hideyuki Kishino
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 445:289-93. 2014
    ..These results indicate that PC containing short acyl residues was remodeled to those with acyl chains of physiological length in yeast. ..
  11. ncbi request reprint Isolation and characterization of a mutant defective in utilization of exogenous phosphatidylethanolamine in Saccharomyces cerevisiae
    Lan Deng
    Department of Biotechnology, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    J Gen Appl Microbiol 53:255-8. 2007
  12. ncbi request reprint Disruption of the SCS2 ortholog in the alkane-assimilating yeast Yarrowia lipolytica impairs its growth on n-decane, but does not impair inositol prototrophy
    Satoshi Kobayashi
    Department of Biotechnology, The University of Tokyo, Japan
    Biosci Biotechnol Biochem 72:2219-23. 2008
    ..The same disruption did not cause inositol auxotrophy, implying that Y. lipolytica SCS2 has a different function from its Saccharomyces cerevisiae counterpart...
  13. doi request reprint An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica
    Napapol Poopanitpan
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biochem Biophys Res Commun 402:731-5. 2010
    ..These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica...
  14. doi request reprint Construction and characterization of a Yarrowia lipolytica mutant lacking genes encoding cytochromes P450 subfamily 52
    Hiroshi Takai
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Fungal Genet Biol 49:58-64. 2012
    ..lipolytica...
  15. ncbi request reprint Human CTP:phosphoethanolamine cytidylyltransferase: enzymatic properties and unequal catalytic roles of CTP-binding motifs in two cytidylyltransferase domains
    Siqi Tian
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biochem Biophys Res Commun 449:26-31. 2014
    ..These results suggest that the N-terminal CT domain of hECT contributes to its catalytic reaction, but C-terminal CT domain does not. ..
  16. ncbi request reprint Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae
    Yusuke Ono
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:750-2. 2009
    ..Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains...
  17. ncbi request reprint The Bax lnhibitor-1 needs a functional electron transport chain for cell death suppression
    Reiko Oshima
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 581:4627-32. 2007
    ..ATP levels were markedly decreased in all these mutants, suggesting that BI-1 requires normal electron transport activity to suppress cell death in yeast...
  18. ncbi request reprint Construction of a yeast strain with regulatable phospholipid synthesis for analysis of the uptake and metabolism of phosphatidylethanolamine with short acyl chains
    Lan Deng
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biosci Biotechnol Biochem 71:2313-5. 2007
    ..Our system, which employs TKY12Ga strain and diC10PE, provides a valuable tool to study the transport and metabolism of PE in yeast...
  19. doi request reprint Incorporation and remodeling of extracellular phosphatidylcholine with short acyl residues in Saccharomyces cerevisiae
    Kunihiko Tanaka
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo, 113 8657, Japan
    Biochim Biophys Acta 1781:391-9. 2008
    ..The current study provides a pivotal insight into the acyl chain remodeling of phospholipids in yeast...
  20. pmc Crystallization and preliminary X-ray analysis of CTP:phosphoethanolamine cytidylyltransferase (ECT) from Saccharomyces cerevisiae
    Jun Ohtsuka
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:1003-5. 2006
    ..3, c = 150.8 A. The crystals contain one ECT molecule in the asymmetric unit (V(M) = 2.2 A(3) Da(-1)), with a solvent content of 43%...
  21. ncbi request reprint Genome-wide screening of aluminum tolerance in Saccharomyces cerevisiae
    Masayuki Kakimoto
    Department of Applied Biological Chemistry, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Biometals 18:467-74. 2005
    ..The genes for protein mannosylation are also associated with Al tolerance, demonstrating the importance of cell wall architecture. These genes are involved in cell integrity processes...
  22. ncbi request reprint Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors
    Haeng Seog Lee
    Department of Biotechnology, University of Tokyo, Japan
    Biosci Biotechnol Biochem 70:521-4. 2006
    ....
  23. ncbi request reprint Identification and characterization of a gene encoding an ABC transporter expressed in the dicarboxylic acid-producing yeast Candida maltosa
    Yoshiyuki Sagehashi
    Department of Biotechnology, The University of Tokyo
    Biosci Biotechnol Biochem 77:2502-4. 2013
    ..maltosa in a later phase of culture on n-dodecane, but not in its parental strain. CmCDR1 expression was significantly induced by the longer-chain DCA in this mutant. ..
  24. ncbi request reprint Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity
    Kunihiko Iwamoto
    Department of Biotechnology, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Genes Cells 9:891-903. 2004
    ....
  25. ncbi request reprint A basic helix-loop-helix transcription factor essential for cytochrome p450 induction in response to alkanes in yeast Yarrowia lipolytica
    Setsu Yamagami
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo, 113 8657, Japan
    J Biol Chem 279:22183-9. 2004
    ..Yas1p also binds to its own promoter, and the expression of YAS1 is induced by n-alkanes. These features suggest that Yas1p is a novel transcription factor mediating alkane signaling and that it provides an autoregulatory loop...
  26. ncbi request reprint [Relationship between localized phosphatidylethanolamine exposure and yeast cell polarity]
    Kunihiko Iwamoto
    Tanpakushitsu Kakusan Koso 49:1367-72. 2004