Kunihiro Sakuma

Summary

Affiliation: Toyohasi University of Technology
Location: Toyohashi, Japan
URL: http://www.health.tut.ac.jp/sakuma/index.html
Summary:
I graduated from the doctoral program in University of Tsukuba, Japan 1996. From 1996 to 2000, I worked as researcher in Department of Physiology, Institute for Developmental Research of Aichi Human Service Center. At this time, I studied mainly the molecular mechanism of merosin-deficient congenital muscular dystrophy. From 2000 to 2005, I worked as assistant professor, Department of Legal Medicine, Kyoto Prefectural University of Medicine. In this place, my major concern was the functional role of calcineurin on muscle regeneration and hypertrophy. My research interests, now in the Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, focus on molecular mechanism of sarcopenia and this attenuating strategy (nutrient, pharmaceutical, exercise, etc), and particularly on autophagy process in sarcopenia. I am author of about 50 papers published in international journals and invited book chapters. I participated in the member of Editorial Board of several journal (International Journal of Biomedical Science, World Journal of Neurology, etc).
Grants:
2011  Research Grant-in-Aid for Scientific Research C
(No. 23500578)
2008-2010 Research Grant-in-Aid for Scientific Research C
(No. 20500575)
2005-2006 Research Grant-in-Aid for Young Scientists B
(No. 17700500)
2003-2004 Research Grant-in-Aid for Young Scientists B
(No. 15700423)
2001-2002 Research Grant-in-Aid for Encouraging Research
A (No. 13780029)
1999-2000 Research Grant-in-Aid for Encouraging Research
A (No. 11780059)

from the Ministry of Education, Culture, Sports,
Science and Technology of Japan

Publications

  1. ncbi request reprint Molecular mechanisms in aging and current strategies to counteract sarcopenia
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Japan
    Curr Aging Sci 3:90-101. 2010
  2. pmc The recent understanding of the neurotrophin's role in skeletal muscle adaptation
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    J Biomed Biotechnol 2011:201696. 2011
  3. ncbi request reprint Changes in PKB/Akt and calcineurin signaling during recovery in atrophied soleus muscle induced by unloading
    Takao Sugiura
    Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University, Japan
    Am J Physiol Regul Integr Comp Physiol 288:R1273-8. 2005
  4. ncbi request reprint Increase of Cardiotrophin-1 immunoreactivity in regenerating and overloaded but not denervated muscles of rats
    Junji Nishikawa
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, Japan
    Neuropathology 25:54-65. 2005
  5. ncbi request reprint Prolactin receptor knockdown in the rat paraventricular nucleus by a morpholino-antisense oligonucleotide causes hypocalcemia and stress gastric erosion
    Takahiko Fujikawa
    Department of Biochemistry and Proteomics, Institute of Genomics and Regenerative Biology, Mie University Graduate School of Medicine, Tsu, Mie 514 8507, Japan
    Endocrinology 146:3471-80. 2005
  6. ncbi request reprint Cyclosporin A treatment upregulates Id1 and Smad3 expression and delays skeletal muscle regeneration
    Kunihiro Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, 602 8566 Kyoto, Japan
    Acta Neuropathol 110:269-80. 2005
  7. ncbi request reprint Normal distribution of presenilin-1 and nicastrin in skeletal muscle and the differential responses of these proteins after denervation
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1, Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Biochim Biophys Acta 1760:980-7. 2006
  8. ncbi request reprint The expression of IGF-I and myostatin mRNAs in skeletal muscle of hypophysectomized and underfed rats during postnatal growth
    A Yamaguchi
    School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Ishikari Tobetsu, Hokkaido, Japan
    Acta Physiol (Oxf) 186:291-300. 2006
  9. ncbi request reprint Increased expression of neuregulin-1 in differentiating muscle satellite cells and in motoneurons during muscle regeneration
    Miyuki Hirata
    Department of Orthopedics, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, Kyoto, 602 8566, Japan
    Acta Neuropathol 113:451-9. 2007
  10. doi request reprint Cyclosporin A modulates cellular localization of MEF2C protein and blocks fiber hypertrophy in the overloaded soleus muscle of mice
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Acta Neuropathol 115:663-74. 2008

Collaborators

Detail Information

Publications43

  1. ncbi request reprint Molecular mechanisms in aging and current strategies to counteract sarcopenia
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Japan
    Curr Aging Sci 3:90-101. 2010
    ..Resistance training in combination with amino acid-containing nutrition would be the best candidate to attenuate, prevent, or ultimately reverse age-related muscle wasting and weakness...
  2. pmc The recent understanding of the neurotrophin's role in skeletal muscle adaptation
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    J Biomed Biotechnol 2011:201696. 2011
    ..This paper provides a basis for a better understanding of the role of these factors under such pathological conditions and for treatment of human neuromuscular disease...
  3. ncbi request reprint Changes in PKB/Akt and calcineurin signaling during recovery in atrophied soleus muscle induced by unloading
    Takao Sugiura
    Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University, Japan
    Am J Physiol Regul Integr Comp Physiol 288:R1273-8. 2005
    ..Our data support the concept that CaN is involved in muscle remodeling during the later phases of recovery from disuse muscle atrophy...
  4. ncbi request reprint Increase of Cardiotrophin-1 immunoreactivity in regenerating and overloaded but not denervated muscles of rats
    Junji Nishikawa
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, Japan
    Neuropathology 25:54-65. 2005
    ..We therefore believe that CT-1 plays a key role in regeneration and hypertrophy in the skeletal muscle of rats...
  5. ncbi request reprint Prolactin receptor knockdown in the rat paraventricular nucleus by a morpholino-antisense oligonucleotide causes hypocalcemia and stress gastric erosion
    Takahiko Fujikawa
    Department of Biochemistry and Proteomics, Institute of Genomics and Regenerative Biology, Mie University Graduate School of Medicine, Tsu, Mie 514 8507, Japan
    Endocrinology 146:3471-80. 2005
    ..These results suggest that PRL acting on the PRLR-L in the PVN is one of the critical pathways for regulating circulating Ca2+ levels under both acute stress and nonstress conditions...
  6. ncbi request reprint Cyclosporin A treatment upregulates Id1 and Smad3 expression and delays skeletal muscle regeneration
    Kunihiro Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, 602 8566 Kyoto, Japan
    Acta Neuropathol 110:269-80. 2005
    ..Our results demonstrated that CsA treatment upregulates Id1 and Smad3 expression and delays skeletal muscle regeneration in vivo...
  7. ncbi request reprint Normal distribution of presenilin-1 and nicastrin in skeletal muscle and the differential responses of these proteins after denervation
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1, Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Biochim Biophys Acta 1760:980-7. 2006
    ..These data suggest that enhanced presenilin-1 protein may play a role in the degeneration and regeneration of skeletal muscle...
  8. ncbi request reprint The expression of IGF-I and myostatin mRNAs in skeletal muscle of hypophysectomized and underfed rats during postnatal growth
    A Yamaguchi
    School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Ishikari Tobetsu, Hokkaido, Japan
    Acta Physiol (Oxf) 186:291-300. 2006
    ..To determine the roles of myostatin and insulin-like growth factor-I (IGF-I) during postnatal growth, we examined IGF-I and myostatin mRNA expression in the skeletal muscles of hypophysectomized and underfed rats during postnatal growth...
  9. ncbi request reprint Increased expression of neuregulin-1 in differentiating muscle satellite cells and in motoneurons during muscle regeneration
    Miyuki Hirata
    Department of Orthopedics, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, Kyoto, 602 8566, Japan
    Acta Neuropathol 113:451-9. 2007
    ..Thus, NRG-1 may play an important role in the differentiation of satellite cells in muscle regeneration, while increased NRG-1 expression in motoneurons may enhance the remodeling of partially damaged axons...
  10. doi request reprint Cyclosporin A modulates cellular localization of MEF2C protein and blocks fiber hypertrophy in the overloaded soleus muscle of mice
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Acta Neuropathol 115:663-74. 2008
    ..Our results demonstrated that CsA treatment modulates the amount and cellular localization of MEF2C protein. The modulation of MEF2C by CsA treatment may inhibit the hypertrophic process in the soleus muscle after mechanical overloading...
  11. doi request reprint Expression of mRNA for specific fibroblast growth factors associates with that of the myogenic markers MyoD and proliferating cell nuclear antigen in regenerating and overloaded rat plantaris muscle
    Y Tanaka
    Laboratory of Human Performance and Fitness, Graduate School of Education, Hokkaido University, Sapporo, Hokkaido, Japan
    Acta Physiol (Oxf) 194:149-59. 2008
    ....
  12. ncbi request reprint Age-related reductions in expression of serum response factor and myocardin-related transcription factor A in mouse skeletal muscles
    Kunihiro Sakuma
    Health Science Center, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Biochim Biophys Acta 1782:453-61. 2008
    ..Senescent mice possessed significantly higher levels of MyoD protein in the cytosol and nucleus. Decreased SRF and MRTF expression may induce the atrophy of skeletal muscle with aging...
  13. ncbi request reprint The expression pattern of PKCtheta in satellite cells of normal and regenerating muscle in the rat
    Seiji Tokugawa
    Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medcicine, Kamigyo ku, Kyoto
    Neuropathology 29:211-8. 2009
    ..After 4 and 6 days, the localization of PKCtheta coincided with those of Pax7 and TGF-beta. Thus, PKCtheta may play an important role in inhibiting differentiation and maintaining the quiescent satellite cells in muscle regeneration...
  14. doi request reprint The adaptive responses in several mediators linked with hypertrophy and atrophy of skeletal muscle after lower limb unloading in humans
    K Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Japan
    Acta Physiol (Oxf) 197:151-9. 2009
    ....
  15. doi request reprint Expression profile of Notch-1 in mechanically overloaded plantaris muscle of mice
    Mai Akiho
    Research Center for Physical Fitness, Sports and Health, Toyohashi, University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi, 441 8580, Japan
    Life Sci 86:59-65. 2010
    ..We investigated the expression pattern of Notch-1 in normal and hypertrophied plantaris muscle of mice...
  16. pmc The functional role of calcineurin in hypertrophy, regeneration, and disorders of skeletal muscle
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    J Biomed Biotechnol 2010:721219. 2010
    ..Activation of calcineurin produces muscular hypertrophy of the slow-twitch soleus muscle but not fast-twitch muscles...
  17. doi request reprint Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles
    Fumi Hokari
    Dept of Health and Nutrition, Niigata Univ of Health and Welfare, Niigata, 1398 Shimami cho, Niigata City, Niigata 950 3198, Japan
    J Appl Physiol (1985) 109:332-40. 2010
    ....
  18. ncbi request reprint Oxidative stress and skeletal muscle dysfunction with aging
    Wataru Aoi
    Laboratory of Health Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan
    Curr Aging Sci 4:101-9. 2011
    ..This article describes the influence of oxidative stress on the progress of age-related muscle dysfunction and reviews the effect of countermeasures such as exercise and diet...
  19. ncbi request reprint Interleukin-8 expression in the esophageal mucosa of patients with gastroesophageal reflux disease
    N Yoshida
    Molecular Gastroenterology and Hepatology, Department of Legal Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Scand J Gastroenterol 39:816-22. 2004
    ..In this study, we examined the expression of mRNA for interleukin (IL)-8, a potent chemokine for neutrophils, in the esophageal mucosa of patients with GERD and compared the results with their endoscopic findings and symptoms...
  20. ncbi request reprint Oxidative stress and delayed-onset muscle damage after exercise
    Wataru Aoi
    Department of Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, 602 8566, Japan
    Free Radic Biol Med 37:480-7. 2004
    ....
  21. ncbi request reprint Marked reduction of focal adhesion kinase, serum response factor and myocyte enhancer factor 2C, but increase in RhoA and myostatin in the hindlimb dy mouse muscles
    Kunihiro Sakuma
    Department of Legal Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, 602 8566 Kyoto, Japan
    Acta Neuropathol 108:241-9. 2004
    ..Enhancing myostatin protein would result in further atrophy and degeneration of muscle fiber in dy mice...
  22. ncbi request reprint Differential adaptations of insulin-like growth factor-I, basic fibroblast growth factor, and leukemia inhibitory factor in the plantaris muscle of rats by mechanical overloading: an immunohistochemical study
    K Sakuma
    Department of Physiology, Aichi Human Service Center, Japan
    Acta Neuropathol 95:123-30. 1998
    ..Furthermore, bFGF appears not to be related to the compensatory hypertrophy produced by overloading...
  23. ncbi request reprint Pathological changes in levels of three small stress proteins, alphaB crystallin, HSP 27 and p20, in the hindlimb muscles of dy mouse
    K Sakuma
    Department of Physiology, Institute for Developmental Research, Aichi Human Service Center, Kamiya cho, Kasugai, Aichi 480 0392, Japan
    Biochim Biophys Acta 1406:162-8. 1998
    ....
  24. ncbi request reprint The adaptive response of MyoD family proteins in overloaded, regenerating and denervated rat muscles
    K Sakuma
    Department of Physiology, Institute for Developmental Research, Aichi Human Service Center, Kamiya cho, Kasugai, Aichi 480 0392, Japan
    Biochim Biophys Acta 1428:284-92. 1999
    ..These results indicate that the fast-type-specific gene expression in muscle is modulated by MyoD and Myf-5 proteins and suggest that myogenin protein plays an important role in the reconstruction of damaged neuromuscular connections...
  25. ncbi request reprint Astaxanthin limits exercise-induced skeletal and cardiac muscle damage in mice
    Wataru Aoi
    Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, 602 0841, Japan
    Antioxid Redox Signal 5:139-44. 2003
    ..Astaxanthin can attenuate exercise-induced damage in mouse skeletal muscle and heart, including an associated neutrophil infiltration that induces further damage...
  26. ncbi request reprint Serum response factor plays an important role in the mechanically overloaded plantaris muscle of rats
    Kunihiro Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, Japan
    Histochem Cell Biol 119:149-60. 2003
    ..These results suggest that the signaling pathway through RhoA-FAK-SRF is important to the differentiation of satellite cells by interacting MyoD and myogenin in the hypertrophied muscle of rats...
  27. ncbi request reprint Calcineurin is a potent regulator for skeletal muscle regeneration by association with NFATc1 and GATA-2
    Kunihiro Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 456 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, Japan
    Acta Neuropathol 105:271-80. 2003
    ..Thus, calcineurin may have an important role in muscle regeneration in association with NFATc1 and GATA-2...
  28. ncbi request reprint The reciprocal change of neurotrophin-4 and glial cell line-derived neurotrophic factor protein in the muscles, spinal cord and cerebellum of the dy mouse
    Kunihiro Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 456 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, Kyoto 602 8566, Japan
    Acta Neuropathol 104:482-92. 2002
    ..Furthermore, dy mice include neurogenic abnormalities in the cerebellum and spinal cord along with myogenic disorder of muscle fibers...
  29. ncbi request reprint A possible role for BDNF, NT-4 and TrkB in the spinal cord and muscle of rat subjected to mechanical overload, bupivacaine injection and axotomy
    K Sakuma
    Department of Legal Medicine, Kyoto Prefectural University of Medicine, 465 Kajii cho, Kawaramachi Hirokoji, Kamigyo ku, 602 8566, Kyoto, Japan
    Brain Res 907:1-19. 2001
    ..Furthermore, by interacting with BDNF and NT-4, the TrkB in the spinal cord may be important for the survival of motoneurons and outgrowth of injured peripheral axons following muscle damage...
  30. ncbi request reprint Differential adaptation of growth and differentiation factor 8/myostatin, fibroblast growth factor 6 and leukemia inhibitory factor in overloaded, regenerating and denervated rat muscles
    K Sakuma
    Department of Physiology, Institute for Developmental Research, Aichi Human Service Center, Kamiya cho, Kasugai, Aichi, Japan
    Biochim Biophys Acta 1497:77-88. 2000
    ..The marked reduction of FGF6 in the hypertrophied and regenerating muscle would imply that FGF6 regulates muscle differentiation but not proliferation of satellite cells and/or myoblasts...
  31. ncbi request reprint The adaptive response of transforming growth factor-beta 2 and -beta RII in the overloaded, regenerating and denervated muscles of rats
    K Sakuma
    Department of Physiology, Aichi Human Service Center, Japan
    Acta Neuropathol 99:177-85. 2000
    ..These results indicate that TGF-beta RII is preferentially distributed in fast-type muscles. Furthermore, TGF-beta 2 may play an important role in muscle hypertrophy and regeneration by the usage of TGF-beta RII...
  32. ncbi request reprint Postnatal profiles of myogenic regulatory factors and the receptors of TGF-beta 2, LIF and IGF-I in the gastrocnemius and rectus femoris muscles of dy mouse
    K Sakuma
    Department of Physiology, Aichi Human Service Center, Japan
    Acta Neuropathol 99:169-76. 2000
    ..Therefore, unlike the situation in mdx mice, the milieu of regeneration following repetitive damage seems to be degraded in the muscles of dy mice...
  33. pmc Novel intriguing strategies attenuating to sarcopenia
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    J Aging Res 2012:251217. 2012
    ..In contrast, muscle loss with age may not be influenced positively by treatment with a proteasome inhibitor or antioxidant...
  34. pmc Sarcopenia and age-related endocrine function
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Int J Endocrinol 2012:127362. 2012
    ..Ghrelin is an interesting candidate because it is orally active, avoiding the need for injections. A more comprehensive knowledge of vitamin-D-related mechanisms is needed to utilize this nutrient to prevent sarcopenia...
  35. pmc Sarcopenic obesity and endocrinal adaptation with age
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi 441 8580, Japan
    Int J Endocrinol 2013:204164. 2013
    ..In this review, we describe the possible influence of endocrinal changes with age on sarcopenic obesity...
  36. pmc Sarcopenia and cachexia: the adaptations of negative regulators of skeletal muscle mass
    Kunihiro Sakuma
    Research Center for Physical Fitness, Sports and Health, Toyohashi University of Technology, 1 1 Hibarigaoka, Tenpaku cho, Toyohashi, 441 8580, Japan
    J Cachexia Sarcopenia Muscle 3:77-94. 2012
    ..This review provides an overview of the adaptive changes in negative regulators of muscle mass in both sarcopenia and cachexia...