Research Topics
| Toshiyuki SakakiSummaryAffiliation: Toyama Prefectural University Country: Japan Publications
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Detail Information
Publications
Metabolism of vitamin D3 by cytochromes P450Toshiyuki Sakaki
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
Front Biosci 10:119-34. 2005....- A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serumMasaki Kamakura
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
Protein Expr Purif 45:307-14. 2006..It also activated mitogen-activated protein kinase, as well as protein kinase B, which is a key regulator of cell survival. These findings suggest that p56kP-4 has a growth factor-like action on primary-cultured rat hepatocytes... 
Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450Toshiyuki Sakaki
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Biochim Biophys Acta 1814:249-56. 2011..Therefore, we believe that the bioconversion of vitamin D and its analogs is one of the most promising P450 reactions in terms of practical application...- Practical application of cytochrome P450Toshiyuki Sakaki
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Japan
Biol Pharm Bull 35:844-9. 2012..The P450 superfamily has tremendous potential for practical applications in various fields... - Enzyme systems for biodegradation of polychlorinated dibenzo-p-dioxinsToshiyuki Sakaki
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939 0398, Japan
Appl Microbiol Biotechnol 88:23-30. 2010..We hope that recombinant microorganisms harboring genetically engineered dioxin-metabolizing enzymes will be used for bioremediation of soil contaminated with PCDDs and polychlorinated dibenzofurans in the near future... - Comparison of metabolism of sesamin and episesamin by drug-metabolizing enzymes in human liverKaori Yasuda
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
Drug Metab Dispos 40:1917-26. 2012..In this study, we have revealed significantly different metabolism by P450, UDP-glucuronosyltransferase, and catechol-O-methyltransferase for sesamin and episesamin, resulting in different biological effects... - Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete chrysosporiumNoriyuki Kasai
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, Japan
J Biochem 147:117-25. 2010..It is noted that these unique P. chrysosporium P450s have a potential for the production of useful flavonoids... - Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysisKeiko Hayashi
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan
FEBS J 277:3999-4009. 2010..In conclusion, we have demonstrated that R73V/R84A can catalyze hydroxylations at C25, C1 and C26 (C27) positions of vitamin D(3) to produce biologically useful compounds... - Sequential metabolism of sesamin by cytochrome P450 and UDP-glucuronosyltransferase in human liverKaori Yasuda
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Drug Metab Dispos 39:1538-45. 2011..On the basis of these results, we concluded that CYP2C9, UGT2B7, and COMT played essential roles in the metabolism of sesamin in the human liver... - Structure-based design of a highly active vitamin D hydroxylase from Streptomyces griseolus CYP105A1Keiko Hayashi
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Biochemistry 47:11964-72. 2008.... - Human cytochrome P450-dependent differential metabolism among three 2α-substituted-1α,25-dihydroxyvitamin D(3) analogsKaori Yasuda
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
J Steroid Biochem Mol Biol 133:84-92. 2013..These results suggest that modification at C-2α position and C-19 demethylenation markedly change metabolic profiles and biological activities of vitamin D analogs... - Mechanism of the anti-proliferative action of 25-hydroxy-19-nor-vitamin D(3) in human prostate cellsEiji Munetsuna
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
J Mol Endocrinol 47:209-18. 2011.... - Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450Noriyuki Kasai
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama, Japan
Appl Microbiol Biotechnol 86:773-80. 2010..Although PcCYP11a3 showed no detectable activity toward 2,7-dichloro-dibenzop-dioxin and 2,3,7-trichloro-dibenzo-p-dioxin, PcCYP11a3 is promising as a template whose activity would be enhanced by site-directed mutagenesis... - Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporiumNoriyuki Kasai
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, Japan
Biochem Biophys Res Commun 387:103-8. 2009..These results suggest that PcCYP65a2 is practically useful for both bioconversion and bioremediation... - Metabolism of 1alpha,25-dihydroxyvitamin D2 by human CYP24A1Naoko Urushino
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Biochem Biophys Res Commun 384:144-8. 2009..The combination of hydroxylation at multiple sites and C-C bond cleavage could form a large number of metabolites. Our findings appear to be useful to predict the metabolism of vitamin D2 and its analogs in the human body... - How is sesamin metabolised in the human liver to show its biological effects?Kaori Yasuda
Toyama Prefectural University, Department of Biotechnology, Faculty of Engineering, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Expert Opin Drug Metab Toxicol 8:93-102. 2012..Furthermore, further analysis of sesamin and episesamin and their molecular mechanisms are needed to make better use of sesamin supplements... - Changes in hepatic gene expression associated with the hypocholesterolaemic activity of royal jellyMasaki Kamakura
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
J Pharm Pharmacol 58:1683-9. 2006..Thus, the hypocholesterolaemic action of RJ appears to be associated with a decrease of SQLE and an increase of LDLR in mice... - Metabolism of sesamin by cytochrome P450 in human liver microsomesKaori Yasuda
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Drug Metab Dispos 38:2117-23. 2010..Our findings strongly suggest that further studies are needed to reveal the interaction between sesamin and therapeutic drugs mainly metabolized by CYP2C9... - Metabolism of the c-Fos/activator protein-1 inhibitor T-5224 by multiple human UDP-glucuronosyltransferase isoformsShinsuke Uchihashi
Research Laboratories, Toyama Chemical Co, Ltd, Toyama, Japan
Drug Metab Dispos 39:803-13. 2011..In this study, we obtained useful information on the metabolism of T-5224 for its clinical use... - Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and ratsHiromi Hamamoto
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Mol Pharmacol 70:120-8. 2006..Our findings provide useful information for the development of new vitamin D analogs for clinical use... - [Recent studies on vitamin D metabolizing enzymes]Toshiyuki Sakaki
Toyama Prefectural University, Faculty of Engineering, Department of Biotechnology
Clin Calcium 16:1129-35. 2006..Some vitamin D analogs are good substrates for CYP3A1 while CYP24A1 is responsible for metabolism of most of vitamin D analogs... - Increase of AMPA receptor glutamate receptor 1 subunit and B-cell receptor-associated protein 31 gene expression in hippocampus of fatigued miceMasaki Kamakura
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
Neurosci Lett 387:1-4. 2005..These results suggest that a compositional change of AMPA receptor (increase of GluR1) and upregulation of the Bap31 gene may be implicated in fatigue in mice... - Possibility of application of cytochrome P450 to bioremediation of dioxinsToshiyuki Sakaki
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
Biotechnol Appl Biochem 60:65-70. 2013.... - The critical role of amino acid residue at position 117 of mouse UDP-glucuronosyltransfererase 1a6a and 1a6b in resveratrol glucuronidationShinsuke Uchihashi
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
J Biochem 152:331-40. 2012.... - Functional analyses of cytochrome P450 genes responsible for the early steps of brassicicene C biosynthesisMakoto Hashimoto
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939 0398, Japan
Bioorg Med Chem Lett 19:5640-3. 2009..Based on the structures of the compounds produced by the transformants, Orf1 is suggested to be an 8beta-hydroxylation enzyme that yields FD 8beta-ol (4), followed by 16-hydroxylation by Orf7 to produce FD 8beta16-diol (5)... - Monospecific antipeptide antibodies against human hepatic UDP-glucuronosyltransferase 1A subfamily (UGT1A) isoformsShin ichi Ikushiro
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan
Drug Metab Pharmacokinet 21:70-4. 2006..These highly specific antipeptide antibodies provide an important tool to analyze tissue distribution and interindividual expression levels of human UGT1As... - Influence of royal jelly on mouse hepatic gene expression and safety assessment with a DNA microarrayMasaki Kamakura
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
J Nutr Sci Vitaminol (Tokyo) 51:148-55. 2005..We suggest that application of DNA microarray technology to the biochemical evaluation of food safety may be effective for rapid and precise quality control...