Toshiyuki Sakaki

Summary

Affiliation: Toyama Prefectural University
Country: Japan

Publications

  1. ncbi request reprint Metabolism of vitamin D3 by cytochromes P450
    Toshiyuki Sakaki
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
    Front Biosci 10:119-34. 2005
  2. ncbi request reprint A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum
    Masaki Kamakura
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
    Protein Expr Purif 45:307-14. 2006
  3. doi request reprint Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Biochim Biophys Acta 1814:249-56. 2011
  4. ncbi request reprint CYP24A1 as a potential target for cancer therapy
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University Imizu, Toyama 939 0398, Japan
    Anticancer Agents Med Chem 14:97-108. 2014
  5. ncbi request reprint Possibility of application of cytochrome P450 to bioremediation of dioxins
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
    Biotechnol Appl Biochem 60:65-70. 2013
  6. ncbi request reprint Practical application of cytochrome P450
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Japan
    Biol Pharm Bull 35:844-9. 2012
  7. doi request reprint Enzyme systems for biodegradation of polychlorinated dibenzo-p-dioxins
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939 0398, Japan
    Appl Microbiol Biotechnol 88:23-30. 2010
  8. doi request reprint Comparison of metabolism of sesamin and episesamin by drug-metabolizing enzymes in human liver
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
    Drug Metab Dispos 40:1917-26. 2012
  9. doi request reprint Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysis
    Keiko Hayashi
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan
    FEBS J 277:3999-4009. 2010
  10. ncbi request reprint Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete chrysosporium
    Noriyuki Kasai
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, Japan
    J Biochem 147:117-25. 2010

Collaborators

Detail Information

Publications31

  1. ncbi request reprint Metabolism of vitamin D3 by cytochromes P450
    Toshiyuki Sakaki
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
    Front Biosci 10:119-34. 2005
    ....
  2. ncbi request reprint A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum
    Masaki Kamakura
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
    Protein Expr Purif 45:307-14. 2006
    ..It also activated mitogen-activated protein kinase, as well as protein kinase B, which is a key regulator of cell survival. These findings suggest that p56kP-4 has a growth factor-like action on primary-cultured rat hepatocytes...
  3. doi request reprint Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Biochim Biophys Acta 1814:249-56. 2011
    ..Therefore, we believe that the bioconversion of vitamin D and its analogs is one of the most promising P450 reactions in terms of practical application...
  4. ncbi request reprint CYP24A1 as a potential target for cancer therapy
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University Imizu, Toyama 939 0398, Japan
    Anticancer Agents Med Chem 14:97-108. 2014
    ....
  5. ncbi request reprint Possibility of application of cytochrome P450 to bioremediation of dioxins
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
    Biotechnol Appl Biochem 60:65-70. 2013
    ....
  6. ncbi request reprint Practical application of cytochrome P450
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Japan
    Biol Pharm Bull 35:844-9. 2012
    ..The P450 superfamily has tremendous potential for practical applications in various fields...
  7. doi request reprint Enzyme systems for biodegradation of polychlorinated dibenzo-p-dioxins
    Toshiyuki Sakaki
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939 0398, Japan
    Appl Microbiol Biotechnol 88:23-30. 2010
    ..We hope that recombinant microorganisms harboring genetically engineered dioxin-metabolizing enzymes will be used for bioremediation of soil contaminated with PCDDs and polychlorinated dibenzofurans in the near future...
  8. doi request reprint Comparison of metabolism of sesamin and episesamin by drug-metabolizing enzymes in human liver
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Toyama, Japan
    Drug Metab Dispos 40:1917-26. 2012
    ..In this study, we have revealed significantly different metabolism by P450, UDP-glucuronosyltransferase, and catechol-O-methyltransferase for sesamin and episesamin, resulting in different biological effects...
  9. doi request reprint Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysis
    Keiko Hayashi
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan
    FEBS J 277:3999-4009. 2010
    ..In conclusion, we have demonstrated that R73V/R84A can catalyze hydroxylations at C25, C1 and C26 (C27) positions of vitamin D(3) to produce biologically useful compounds...
  10. ncbi request reprint Atypical kinetics of cytochromes P450 catalysing 3'-hydroxylation of flavone from the white-rot fungus Phanerochaete chrysosporium
    Noriyuki Kasai
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, Japan
    J Biochem 147:117-25. 2010
    ..It is noted that these unique P. chrysosporium P450s have a potential for the production of useful flavonoids...
  11. doi request reprint Sequential metabolism of sesamin by cytochrome P450 and UDP-glucuronosyltransferase in human liver
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Drug Metab Dispos 39:1538-45. 2011
    ..On the basis of these results, we concluded that CYP2C9, UGT2B7, and COMT played essential roles in the metabolism of sesamin in the human liver...
  12. doi request reprint Structure-based design of a highly active vitamin D hydroxylase from Streptomyces griseolus CYP105A1
    Keiko Hayashi
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Biochemistry 47:11964-72. 2008
    ....
  13. ncbi request reprint Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells
    Eiji Munetsuna
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan Department of Biochemistry, Fujita Health University for Medical Science, Toyoake 470 1192, Japan
    Mol Cell Endocrinol 382:960-70. 2014
    ..DNA microarray and real-time RT-PCR analyses suggest that semaphorin 3B, cystatin E/M, and cystatin D may be involved in the antiproliferative effect of 25(OH)D3. ..
  14. doi request reprint Human cytochrome P450-dependent differential metabolism among three 2α-substituted-1α,25-dihydroxyvitamin D(3) analogs
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    J Steroid Biochem Mol Biol 133:84-92. 2013
    ..These results suggest that modification at C-2α position and C-19 demethylenation markedly change metabolic profiles and biological activities of vitamin D analogs...
  15. ncbi request reprint Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450
    Noriyuki Kasai
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama, Japan
    Appl Microbiol Biotechnol 86:773-80. 2010
    ..Although PcCYP11a3 showed no detectable activity toward 2,7-dichloro-dibenzop-dioxin and 2,3,7-trichloro-dibenzo-p-dioxin, PcCYP11a3 is promising as a template whose activity would be enhanced by site-directed mutagenesis...
  16. doi request reprint Mechanism of the anti-proliferative action of 25-hydroxy-19-nor-vitamin D(3) in human prostate cells
    Eiji Munetsuna
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    J Mol Endocrinol 47:209-18. 2011
    ....
  17. ncbi request reprint Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium
    Noriyuki Kasai
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, Japan
    Biochem Biophys Res Commun 387:103-8. 2009
    ..These results suggest that PcCYP65a2 is practically useful for both bioconversion and bioremediation...
  18. ncbi request reprint The critical role of amino acid residue at position 117 of mouse UDP-glucuronosyltransfererase 1a6a and 1a6b in resveratrol glucuronidation
    Shinsuke Uchihashi
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    J Biochem 152:331-40. 2012
    ....
  19. doi request reprint UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Biochem Biophys Res Commun 434:311-5. 2013
    ..This novel method for producing 25-hydroxyvitamin D2 without a substrate could be useful for practical purposes...
  20. doi request reprint Metabolism of 1alpha,25-dihydroxyvitamin D2 by human CYP24A1
    Naoko Urushino
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Biochem Biophys Res Commun 384:144-8. 2009
    ..The combination of hydroxylation at multiple sites and C-C bond cleavage could form a large number of metabolites. Our findings appear to be useful to predict the metabolism of vitamin D2 and its analogs in the human body...
  21. doi request reprint How is sesamin metabolised in the human liver to show its biological effects?
    Kaori Yasuda
    Toyama Prefectural University, Department of Biotechnology, Faculty of Engineering, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Expert Opin Drug Metab Toxicol 8:93-102. 2012
    ..There is currently a need to identify the enzymes responsible for metabolism of sesamin so that scientists will be more able to predict sesamin-drug interactions...
  22. doi request reprint Metabolism of the c-Fos/activator protein-1 inhibitor T-5224 by multiple human UDP-glucuronosyltransferase isoforms
    Shinsuke Uchihashi
    Research Laboratories, Toyama Chemical Co, Ltd, Toyama, Japan
    Drug Metab Dispos 39:803-13. 2011
    ..In this study, we obtained useful information on the metabolism of T-5224 for its clinical use...
  23. ncbi request reprint Metabolism of sesamin by cytochrome P450 in human liver microsomes
    Kaori Yasuda
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Drug Metab Dispos 38:2117-23. 2010
    ..Our findings strongly suggest that further studies are needed to reveal the interaction between sesamin and therapeutic drugs mainly metabolized by CYP2C9...
  24. ncbi request reprint Changes in hepatic gene expression associated with the hypocholesterolaemic activity of royal jelly
    Masaki Kamakura
    Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    J Pharm Pharmacol 58:1683-9. 2006
    ..Thus, the hypocholesterolaemic action of RJ appears to be associated with a decrease of SQLE and an increase of LDLR in mice...
  25. ncbi request reprint Comparison of serotonin glucuronidation activity of UDP-glucuronosyltransferase 1a6a (Ugt1a6a) and Ugt1a6b: evidence for the preferential expression of Ugt1a6a in the mouse brain
    Shinsuke Uchihashi
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Imizu, Japan
    Drug Metab Pharmacokinet 28:260-4. 2013
    ..Our new findings indicate that Ugt1a6a and Ugt1a6b play different roles in mice, driven by differences in expression and kinetic properties for serotonin glucuronidation...
  26. ncbi request reprint Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats
    Hiromi Hamamoto
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    Mol Pharmacol 70:120-8. 2006
    ..Our findings provide useful information for the development of new vitamin D analogs for clinical use...
  27. ncbi request reprint [Recent studies on vitamin D metabolizing enzymes]
    Toshiyuki Sakaki
    Toyama Prefectural University, Faculty of Engineering, Department of Biotechnology
    Clin Calcium 16:1129-35. 2006
    ..Some vitamin D analogs are good substrates for CYP3A1 while CYP24A1 is responsible for metabolism of most of vitamin D analogs...
  28. ncbi request reprint Increase of AMPA receptor glutamate receptor 1 subunit and B-cell receptor-associated protein 31 gene expression in hippocampus of fatigued mice
    Masaki Kamakura
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
    Neurosci Lett 387:1-4. 2005
    ..These results suggest that a compositional change of AMPA receptor (increase of GluR1) and upregulation of the Bap31 gene may be implicated in fatigue in mice...
  29. doi request reprint Functional analyses of cytochrome P450 genes responsible for the early steps of brassicicene C biosynthesis
    Makoto Hashimoto
    Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939 0398, Japan
    Bioorg Med Chem Lett 19:5640-3. 2009
    ..Based on the structures of the compounds produced by the transformants, Orf1 is suggested to be an 8beta-hydroxylation enzyme that yields FD 8beta-ol (4), followed by 16-hydroxylation by Orf7 to produce FD 8beta16-diol (5)...
  30. ncbi request reprint Monospecific antipeptide antibodies against human hepatic UDP-glucuronosyltransferase 1A subfamily (UGT1A) isoforms
    Shin ichi Ikushiro
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan
    Drug Metab Pharmacokinet 21:70-4. 2006
    ..These highly specific antipeptide antibodies provide an important tool to analyze tissue distribution and interindividual expression levels of human UGT1As...
  31. ncbi request reprint Influence of royal jelly on mouse hepatic gene expression and safety assessment with a DNA microarray
    Masaki Kamakura
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
    J Nutr Sci Vitaminol (Tokyo) 51:148-55. 2005
    ..We suggest that application of DNA microarray technology to the biochemical evaluation of food safety may be effective for rapid and precise quality control...