Research Topics
| H OnakaSummaryAffiliation: Toyama Prefectural University Country: Japan Publications
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Detail Information
Publications
Cloning of the staurosporine biosynthetic gene cluster from Streptomyces sp. TP-A0274 and its heterologous expression in Streptomyces lividansHiroyasu Onaka
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
J Antibiot (Tokyo) 55:1063-71. 2002....
Mycolic acid-containing bacteria induce natural-product biosynthesis in Streptomyces speciesHiroyasu Onaka
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama 939 0398, Japan
Appl Environ Microbiol 77:400-6. 2011..We used these results to develop a new coculture method, called the combined-culture method, which facilitates the screening of natural products...- Characterization of the biosynthetic gene cluster of rebeccamycin from Lechevalieria aerocolonigenes ATCC 39243Hiroyasu Onaka
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
Biosci Biotechnol Biochem 67:127-38. 2003.... - pTOYAMAcos, pTYM18, and pTYM19, actinomycete-Escherichia coli integrating vectors for heterologous gene expressionHiroyasu Onaka
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
J Antibiot (Tokyo) 56:950-6. 2003..L. aerocolonigenes deltarebC strain, which is defective in rebeccamycin production because of a rebC deletion, was restored to rebeccamycin production by complemention by rebC cloned in pTYM 19... - Cloning and characterization of the goadsporin biosynthetic gene cluster from Streptomyces sp. TP-A0584Hiroyasu Onaka
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939 0398, Japan
Microbiology 151:3923-33. 2005..Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of godA, suggesting that this biosynthesis machinery is used for the heterocyclization of peptides... - Biosynthesis of indolocarbazole and goadsporin, two different heterocyclic antibiotics produced by actinomycetesHiroyasu Onaka
Department of Biotechnology, Faculty of Engineering and Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama, Japan
Biosci Biotechnol Biochem 73:2149-55. 2009..On the basis of these knowledges, a wide variety of indolocarbazole and goadsporin analogs through the rational gene recombination and disruption of these biosynthetic genes were successfully produced... - Cytochrome P450 homolog is responsible for C-N bond formation between aglycone and deoxysugar in the staurosporine biosynthesis of Streptomyces sp. TP-A0274Hiroyasu Onaka
Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
Biosci Biotechnol Biochem 69:1753-9. 2005.... - Goadsporin, a chemical substance which promotes secondary metabolism and morphogenesis in streptomycetes. I. Purification and characterizationH Onaka
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan
J Antibiot (Tokyo) 54:1036-44. 2001..These results suggest that goadsporin acts on a common regulation pathway for sporulation and secondary metabolism in streptomycetes and can be a powerful tool to analyze the regulation mechanism... - Cloning and characterization of the A-factor receptor gene from Streptomyces griseusH Onaka
Department of Biotechnology, University of Tokyo, Japan
J Bacteriol 177:6083-92. 1995..The presence of ArpA-like proteins among Streptomyces spp., as revealed by PCR, together with the presence of A-factor-like compounds, suggests that a hormonal control similar to the A-factor system exists in many species of this genus... - Site-directed mutagenesis of the A-factor receptor protein: Val-41 important for DNA-binding and Trp-119 important for ligand-bindingM Sugiyama
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
Gene 222:133-44. 1998..However, neither CprA nor CprB recognized A-factor, probably due to much less similarity in the C-terminal domains... - DNA-binding activity of the A-factor receptor protein and its recognition DNA sequencesH Onaka
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Bunkyo ku, Japan
Mol Microbiol 24:991-1000. 1997..A-factor, produced in a growth-dependent manner, releases ArpA from the DNA, thus switching on the expression of the key gene(s), leading to the onset of secondary metabolism and aerial mycelium formation... - A mutation at proline-115 in the A-factor receptor protein of Streptomyces griseus abolishes DNA-binding ability but not ligand-binding abilityH Onaka
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Japan
J Bacteriol 179:2748-52. 1997..This is in agreement with the idea that A-factor binding to the COOH-terminal domain of ArpA causes a subtle conformational change of the distal NH2-terminal DNA-binding domain, resulting in dissociation of ArpA from DNA... - Goadsporin, a chemical substance which promotes secondary metabolism and Morphogenesis in streptomycetes. II. Structure determinationY Igarashi
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan
J Antibiot (Tokyo) 54:1045-53. 2001..The chiral amino acids were determined by the advanced Marfey's method to have L-configurations... - The A-factor regulatory cascade leading to streptomycin biosynthesis in Streptomyces griseus : identification of a target gene of the A-factor receptorY Ohnishi
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
Mol Microbiol 34:102-11. 1999..On the basis of these findings, we propose here a model to demonstrate how A-factor triggers streptomycin biosynthesis at a late exponential growth stage... - Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of secondary metabolism and morphogenesisH Onaka
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan
Mol Microbiol 28:743-53. 1998..It is thus concluded that both cprA and cprB play regulatory roles in both secondary metabolism and morphogenesis in S. coelicolor A3(2), just as the arpA/A-factor system in Streptomyces griseus... - Absolute configuration of TPU-0043, a pentaene macrolide from Streptomyces spYasuhiro Igarashi
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
J Antibiot (Tokyo) 58:523-5. 2005.... - Revision of the structure assigned to the antibiotic BU-4664L from MicromonoporaYasuhiro Igarashi
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939 0398, Japan
J Antibiot (Tokyo) 58:350-2. 2005..was revised to 5,10-dihydro-4,6,8-trihydroxy-10-(3,7,11-trimethyl-trans-2,trans-6,10-dodecatrienyl)-11H-dibenzo[b,e] [1,4]-diazepin-11-one based on the NMR analysis... - Anicemycin, a new inhibitor of anchorage-independent growth of tumor cells from Streptomyces sp. TP-A0648Yasuhiro Igarashi
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Imizu, Toyama 939 0398, Japan
J Antibiot (Tokyo) 58:322-6. 2005..Anicemycin inhibited the anchorage-independent growth of the human ovary cancer SKOV-3 cells with an IC50 of 0.015 microM about three times more potently than their anchorage-dependent growth... 
Alchivemycin A, a bioactive polycyclic polyketide with an unprecedented skeleton from Streptomyces spYasuhiro Igarashi
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Org Lett 12:3402-5. 2010..The new compound contains an unprecedented heterocyclic ring system, 2H-tetrahydro-4,6-dioxo-1,2-oxazine. Alchivemycin A showed potent antimicrobial activity against Micrococcus luteus and inhibitory effects on tumor cell invasion...- Cloning of the gene cluster responsible for biosynthesis of KS-505a (longestin), a unique tetraterpenoidYutaka Hayashi
Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
Biosci Biotechnol Biochem 71:3072-81. 2007..A gene disruption experiment was also employed to confirm that the cluster indeed participated in KS-505a biosynthesis. This is believed to be the first report detailing the gene cluster of a cyclized tetraterpenoid... - Anti-invasive and anti-angiogenic activities of naturally occurring dibenzodiazepine BU-4664L and its derivativesSatoshi Miyanaga
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Bioorg Med Chem Lett 20:963-5. 2010..Compounds 3 and 4 exhibited potent anti-angiogenic effects on HUVEC, together with remarkable inhibition of cell migration at nanomolar concentrations, and showed much lower cytotoxicity... - A copper-containing oxidase catalyzes C-nitrosation in nitrosobenzamide biosynthesisAkio Noguchi
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo, Japan
Nat Chem Biol 6:641-3. 2010..This finding indicates diverse catalytic functions of tyrosinase-like copper-containing monooxygenases in nature... - Synthesis and evaluation of myxochelin analogues as antimetastatic agentsSatoshi Miyanaga
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
Bioorg Med Chem 17:2724-32. 2009..Compound (S)-6 was also shown to inhibit gelatinase activities of MMP-2 and MMP-9 and in vivo lung metastasis in mice... - Absolute configuration and antitumor activity of myxochelin A produced by Nonomuraea pusilla TP-A0861Satoshi Miyanaga
Department of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan
J Antibiot (Tokyo) 59:698-703. 2006..Both enantiomers of myxochelin A showed remarkable inhibitory effects on the invasion of murine colon 26-L5 carcinoma cells at non-cytotoxic concentrations... - Electron transfer activation of chromopyrrolic acid by cytochrome p450 en route to the formation of an antitumor indolocarbazole derivative: theory supports experimentYong Wang
The Institute of Chemistry and the Lise Meitner Minerva Center for Computational Quantum Chemistry, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
J Am Chem Soc 130:7170-1. 2008..The electron transfer is enabled by His250, the presence of carboxylate groups in CPA, and by the H-bonding network that tunes the energetic of the process. Theory supports experiment but reveals some novel aspects of this unusual P450... - Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeletonMasatomo Makino
Biometal Science Laboratory, RIKEN SPring 8 Center, Harima Institute, Hyogo 679 5148, Japan
Proc Natl Acad Sci U S A 104:11591-6. 2007.... - Crystal structure of VioE, a key player in the construction of the molecular skeleton of violaceinSatoshi Hirano
Biometal Science Laboratory, RIKEN SPring 8 Center, Hyogo 679 5148, Japan
J Biol Chem 283:6459-66. 2008..Based on these results, we propose that VioE traps the highly reactive substrate within the surface pocket to suppress CPA formation and promote protodeoxyviolaceinic acid formation caused by proximity and orientation effects...