N Itoh

Summary

Affiliation: Toyama Prefectural University
Country: Japan

Publications

  1. ncbi request reprint Chiral alcohol production by NADH-dependent phenylacetaldehyde reductase coupled with in situ regeneration of NADH
    Nobuya Itoh
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Eur J Biochem 269:2394-402. 2002
  2. ncbi request reprint Characterization of pNI10 plasmid in Pseudomonas, and the construction of an improved Escherichia and Pseudomonas shuttle vector, pNUK73
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, 939 0398, Kosugi, Toyama, Japan
    Appl Microbiol Biotechnol 61:240-6. 2003
  3. ncbi request reprint Continuous production of chiral 1,3-butanediol using immobilized biocatalysts in a packed bed reactor: promising biocatalysis method with an asymmetric hydrogen-transfer bioreduction
    Nobuya Itoh
    Department of Biotechnology, Faculty of Engineering Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Imizu, Toyama 939 0398, Japan
    Appl Microbiol Biotechnol 75:1249-56. 2007
  4. ncbi request reprint Cloning and biochemical characterization of Co(2+)-activated bromoperoxidase-esterase (perhydrolase) from Pseudomonas putida IF-3 strain
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama, Japan
    Biochim Biophys Acta 1545:53-66. 2001
  5. ncbi request reprint Purification and characterization of NADPH-dependent aldo-keto reductase specific for beta-keto esters from Penicillium citrinum, and production of methyl (S)-4-bromo-3-hydroxybutyrate
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa Kosugi, 939 0398 Toyama, Japan
    Appl Microbiol Biotechnol 66:53-62. 2004
  6. ncbi request reprint Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Appl Microbiol Biotechnol 51:193-200. 1999
  7. ncbi request reprint Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli
    J Q Liu
    Laboratory of Biocatalytic Chemistry, Biotechnology Research Centre, Toyama Prefectural University, Japan
    Eur J Biochem 255:220-6. 1998
  8. ncbi request reprint A novel metal-activated pyridoxal enzyme with a unique primary structure, low specificity D-threonine aldolase from Arthrobacter sp. Strain DK-38. Molecular cloning and cofactor characterization
    J Q Liu
    Laboratory of Biocatalytic Chemistry, Biotechnology Research Center, Toyama Prefectural University, Kosugi Machi, Toyama 939 0398, Japan
    J Biol Chem 273:16678-85. 1998
  9. pmc Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin
    T Dairi
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    J Bacteriol 183:6085-94. 2001
  10. ncbi request reprint A novel enzyme, D-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: purification, characterization, and gene cloning
    J Q Liu
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, 939 0398 Kosugi, Toyama, Japan
    Appl Microbiol Biotechnol 62:53-60. 2003

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Chiral alcohol production by NADH-dependent phenylacetaldehyde reductase coupled with in situ regeneration of NADH
    Nobuya Itoh
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Eur J Biochem 269:2394-402. 2002
    ..The high yields were due to the fact that PAR could concomitantly reproduce NADH in the presence of 3-7% (v/v) 2-propanol in the reaction mixture. This biocatalytic process provided one of the best asymmetric reductions ever reported...
  2. ncbi request reprint Characterization of pNI10 plasmid in Pseudomonas, and the construction of an improved Escherichia and Pseudomonas shuttle vector, pNUK73
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, 939 0398, Kosugi, Toyama, Japan
    Appl Microbiol Biotechnol 61:240-6. 2003
    ..68 kb). We show that expression in pseudomonads of the bromoperoxidase gene ( bpo) of Pseudomonas putida, inserted downstream of the lac promoter in pNUK73, resulted in about 30% (13.6 U/l culture) of the enzyme level obtained in E. coli...
  3. ncbi request reprint Continuous production of chiral 1,3-butanediol using immobilized biocatalysts in a packed bed reactor: promising biocatalysis method with an asymmetric hydrogen-transfer bioreduction
    Nobuya Itoh
    Department of Biotechnology, Faculty of Engineering Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Imizu, Toyama 939 0398, Japan
    Appl Microbiol Biotechnol 75:1249-56. 2007
    ..e.) with an STY of 60.3 mg h(-1) ml(-1) catalyst, respectively. The immobilized cells in a packed bed reactor continuously produced (R)-1,3-butanediol with a yield of 99% (about 49.5 g/l) from 5% (w/v) 4-hydroxy-2-butanoate over 500 h...
  4. ncbi request reprint Cloning and biochemical characterization of Co(2+)-activated bromoperoxidase-esterase (perhydrolase) from Pseudomonas putida IF-3 strain
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama, Japan
    Biochim Biophys Acta 1545:53-66. 2001
    ..The results indicated that the enzyme was a novel Co(2+)-activated organic acid-dependent BPO (perhydrolase)-EST, belonging to the non-metal haloperoxidase-hydrolase family...
  5. ncbi request reprint Purification and characterization of NADPH-dependent aldo-keto reductase specific for beta-keto esters from Penicillium citrinum, and production of methyl (S)-4-bromo-3-hydroxybutyrate
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa Kosugi, 939 0398 Toyama, Japan
    Appl Microbiol Biotechnol 66:53-62. 2004
    ..9% enantiomer excess). E. coli cells expressing KER and glucose dehydrogenase in the water/butyl acetate two-phase system achieved a high productivity of (S)-4-bromo-3-hydroxybutyrate (277 mM, 54 mg/ml) in the organic solvent layer...
  6. ncbi request reprint Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production
    N Itoh
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Appl Microbiol Biotechnol 51:193-200. 1999
    ..The dak1 gene was expressed at a high level in Escherichia coli, and the recombinant enzyme was purified to homogeneity and characterized. The acetone powder of recombinant E. coli cells was used to produce dihydroxyacetone phosphate...
  7. ncbi request reprint Gene cloning, biochemical characterization and physiological role of a thermostable low-specificity L-threonine aldolase from Escherichia coli
    J Q Liu
    Laboratory of Biocatalytic Chemistry, Biotechnology Research Centre, Toyama Prefectural University, Japan
    Eur J Biochem 255:220-6. 1998
    ..coli but catalyzes an alternative pathway for cellular glycine when serine hydroxymethyltransferase is inert...
  8. ncbi request reprint A novel metal-activated pyridoxal enzyme with a unique primary structure, low specificity D-threonine aldolase from Arthrobacter sp. Strain DK-38. Molecular cloning and cofactor characterization
    J Q Liu
    Laboratory of Biocatalytic Chemistry, Biotechnology Research Center, Toyama Prefectural University, Kosugi Machi, Toyama 939 0398, Japan
    J Biol Chem 273:16678-85. 1998
    ..Taken together, low specificity D-threonine aldolase from Arthrobacter sp. strain DK-38, with a unique primary structure, is a novel metal-activated pyridoxal enzyme...
  9. pmc Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin
    T Dairi
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    J Bacteriol 183:6085-94. 2001
    ..The two enzymes, each of which was overproduced in Escherichia coli and purified to homogeneity, converted GGDP into terpentetriene. To the best of our knowledge, this is the first report of a eubacterial DC...
  10. ncbi request reprint A novel enzyme, D-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: purification, characterization, and gene cloning
    J Q Liu
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, 939 0398 Kosugi, Toyama, Japan
    Appl Microbiol Biotechnol 62:53-60. 2003
    ..DK-38 and Alcaligenes xylosoxidan IFO 12669, respectively. This is the first paper reporting both a purified enzyme with D-3-hydroxyaspartate aldolase activity and also its gene cloning...
  11. pmc Gene cloning, nucleotide sequencing, and purification and characterization of the low-specificity L-threonine aldolase from Pseudomonas sp. strain NCIMB 10558
    J Q Liu
    Laboratory of Biocatalytic Chemistry, Toyama Prefectural University, Japan
    Appl Environ Microbiol 64:549-54. 1998
    ..Thus, Lys207 of the L-TA probably functions as an essential catalytic residue, forming an internal Schiff base with the pyridoxal 5'-phosphate of the enzyme to catalyze the reversible aldol reaction...
  12. ncbi request reprint Gene cloning and expression of Leifsonia alcohol dehydrogenase (LSADH) involved in asymmetric hydrogen-transfer bioreduction to produce (R)-form chiral alcohols
    Kousuke Inoue
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Biosci Biotechnol Biochem 70:418-26. 2006
    ..The recombinant E. coli cells which overexpressed lsadh produced (R)-form chiral alcohols from ketones using 2-propanol as a hydrogen donor with the highest level of productivity ever reported and enantiomeric excess (e.e.)...
  13. ncbi request reprint Cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway from a terpenoid-antibiotic-producing Streptomyces strain
    Y Hamano
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Biosci Biotechnol Biochem 65:1627-35. 2001
    ..coli. Among them, the recombinant GGDPS, MK, and IPP isomerase were confirmed to have the expected activities. This is the first report, to the best of our knowledge, about eubacterial MK with direct evidence...
  14. pmc Purification and characterization of a novel alcohol dehydrogenase from Leifsonia sp. strain S749: a promising biocatalyst for an asymmetric hydrogen transfer bioreduction
    Kousuke Inoue
    Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
    Appl Environ Microbiol 71:3633-41. 2005
    ..e.), acetophenone to (R)-1-phenylethanol (99% e.e.), and 2-heptanone to (R)-2-heptanol (>99% e.e.) in the presence of 2-propanol without an additional NADH regeneration system. Therefore, it would be a useful biocatalyst...
  15. ncbi request reprint Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis
    N Ohsawa
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Biosci Biotechnol Biochem 65:2397-404. 2001
    ..The enzyme reaction strictly depended on SAM as a methyl donor, and the enzyme catalyzed methylation of the I-, Br-, and Cl- to corresponding monohalomethanes and of bisulfide to methyl mercaptan...
  16. pmc Engineering of phenylacetaldehyde reductase for efficient substrate conversion in concentrated 2-propanol
    Yoshihide Makino
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939 0398, Japan
    Appl Environ Microbiol 71:4713-20. 2005
    ..This method will be widely applicable for the engineering of enzymes potentially valuable for industry...
  17. pmc Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)
    Nobuya Itoh
    Department of Biotechnology, Faculty of Engineering Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama, Japan
    BMC Plant Biol 9:116. 2009
    ....
  18. ncbi request reprint Engineering the phenylacetaldehyde reductase mutant for improved substrate conversion in the presence of concentrated 2-propanol
    Yoshihide Makino
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu shi, Toyama, 939 0398, Japan
    Appl Microbiol Biotechnol 77:833-43. 2007
    ..This mutant is a promising novel biocatalyst for efficient chiral alcohol production...
  19. pmc A knowledge-based structure-discriminating function that requires only main-chain atom coordinates
    Yoshihide Makino
    Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Imizu shi, Toyama 939 0398, Japan
    BMC Struct Biol 8:46. 2008
    ....
  20. ncbi request reprint Brain-specific expression of a novel human UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T9)
    S Toba
    Department of Biotechnology, Faculty of Engineering, Kyto Sangyo University, Kamigamo Motoyama, Kita ku, Kyoto 603 8555, Japan
    Biochim Biophys Acta 1493:264-8. 2000
    ..Northern blot analysis revealed the mRNA expression of the enzyme to be confined to the brain. The brain-specific expression of GalNAc-T9 suggested that this isozyme catalyzes O-glycosylation in the brain...
  21. ncbi request reprint Physiological function of bromoperoxidase in the red marine alga, Corallina pilulifera: production of bromoform as an allelochemical and the simultaneous elimination of hydrogen peroxide
    N Ohsawa
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, 939-0398, Toyama, Japan
    Phytochemistry 58:683-92. 2001
    ..The phenomenon implies that BPO could be a potential substitute for catalase, because the enzyme catalyzes an efficient Br(-)-dependent catalase reaction...
  22. doi request reprint An alternative menaquinone biosynthetic pathway operating in microorganisms
    Tomoshige Hiratsuka
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    Science 321:1670-3. 2008
    ..As humans and commensal intestinal bacteria, including lactobacilli, lack this pathway, it represents an attractive target for the development of chemotherapeutics...
  23. doi request reprint Gene cloning and characterization of dihydrolipoamide dehydrogenase from Microbacterium luteolum: A useful enzymatic regeneration system of NAD+ from NADH
    Junji Kurokawa
    Department of Biotechnology, Faculty of Engineering Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939 0398, Japan
    J Biosci Bioeng 109:218-23. 2010
    ..1 microM). An NAD(+)-regenerating system with MluLPD and laccase using 2,5-dimethoxy-1,4-benzoquinone as a hydrogen acceptor was demonstrated...
  24. ncbi request reprint Growth-phase dependent expression of the mevalonate pathway in a terpenoid antibiotic-producing Streptomyces strain
    Yoshimitsu Hamano
    Biotechnology Research Center, Toyama Prefectural University, Japan
    Biosci Biotechnol Biochem 66:808-19. 2002
    ....
  25. ncbi request reprint Presence of copalyl diphosphate synthase gene in an actinomycete possessing the mevalonate pathway
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Antibiot (Tokyo) 57:739-47. 2004
    ..These results again showed that actinomycetes possessing the mevalonate pathway usually produce an isoprenoid and that its biosynthetic gene cluster exists in adjacent the mevalonate pathway gene cluster...
  26. doi request reprint Cloning of the gene cluster responsible for the biosynthesis of brasilicardin A, a unique diterpenoid
    Yutaka Hayashi
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    J Antibiot (Tokyo) 61:164-74. 2008
    ..We did not, however, detect any genes related to L-rhamnose and N-acetylglucosamine biosyntheses in the flanking regions. A gene disruption experiment did indeed show that this gene cluster was responsible for BCA biosynthesis...
  27. ncbi request reprint Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398
    J Biochem 133:83-91. 2003
    ..These results showed that the amino acid residue at the 57th (58th) position after the FARM also played an important role in determination of the product specificity...
  28. ncbi request reprint A relationship between the mevalonate pathway and isoprenoid production in actinomycetes
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Antibiot (Tokyo) 56:957-66. 2003
    ..This result suggested that the presence of the mevalonate pathway might be a good landmark to detect the production of isoprenoid compounds by actinomycetes...
  29. ncbi request reprint Enzymatic properties of futalosine hydrolase, an enzyme essential to a newly identified menaquinone biosynthetic pathway
    Tomoshige Hiratsuka
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    Biosci Biotechnol Biochem 73:1137-41. 2009
    ..5 and 80 degrees C. The Km value was calculated to be 154.0+/-5.3 microM and the kcat value was 1.02/s. Recombinant TTHA0556 was slightly inhibited by hypoxanthine, with a Ki value of 1.1 mM...
  30. ncbi request reprint Functional analysis of eubacterial ent-copalyl diphosphate synthase and pimara-9(11),15-diene synthase with unique primary sequences
    Chiho Ikeda
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Biochem 141:37-45. 2007
    ..ORF3 is likely to be a monomer and also requires a divalent cation. The optimum pH and temperature were 7.0 and 30 degrees C. The Km value for ent-CDP was estimated to be 2.6 +/- 0.2 microM and the kcat value was 1.4 x 10(-3)/sec...
  31. ncbi request reprint Reaction mechanism of the Co2+-activated multifunctional bromoperoxidase-esterase from Pseudomonas putida IF-3
    Takafumi Kawanami
    Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama, 939 0398, Japan
    Arch Biochem Biophys 398:94-100. 2002
    ..We propose the possible reaction mechanism of this multifunctional enzyme based on these findings...
  32. ncbi request reprint Cloning of the gene cluster responsible for biosynthesis of KS-505a (longestin), a unique tetraterpenoid
    Yutaka Hayashi
    Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
    Biosci Biotechnol Biochem 71:3072-81. 2007
    ..A gene disruption experiment was also employed to confirm that the cluster indeed participated in KS-505a biosynthesis. This is believed to be the first report detailing the gene cluster of a cyclized tetraterpenoid...
  33. ncbi request reprint Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin
    Yoshimitsu Hamano
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Biol Chem 277:37098-104. 2002
    ..These results suggested that an actual reaction catalyzed by the ORF12 was the conversion of TDP into TTE in vivo...
  34. doi request reprint Engineering of NADPH-dependent aldo-keto reductase from Penicillium citrinum by directed evolution to improve thermostability and enantioselectivity
    Hiroyuki Asako
    Organic Synthesis Research Laboratory, Sumitomo Chemical Co, Ltd, Konohana ku, Osaka, Japan
    Appl Microbiol Biotechnol 80:805-12. 2008
    ..An Escherichia coli cell biocatalyst that overproduced the L54Q mutant of KER and glucose dehydrogenase as a cofactor regeneration enzyme showed the highest level of BAM reduction in a water/butyl acetate two-phase system...
  35. pmc Purification and cDNA cloning of NADPH-dependent aldoketoreductase, involved in asymmetric reduction of methyl 4-bromo-3-oxobutyrate, from Penicillium citrinum IFO4631
    Hiroyuki Asako
    Organic Synthesis Research Laboratory, Sumitomo Chemical Co, Ltd, Osaka, Japan
    Appl Environ Microbiol 71:1101-4. 2005
    ..From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system...
  36. ncbi request reprint A vanadium-dependent bromoperoxidase in the marine red alga Kappaphycus alvarezii (Doty) Doty displays clear substrate specificity
    Zornitsa Kamenarska
    Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria
    Phytochemistry 68:1358-66. 2007
    ..The K(m) values for Br- and H2O2 were 2.5x10(-3) M and 8.5x10(-5) M, respectively. The halogenated product, dibromoacetaldehyde, that accumulated in K. alvarezii was additionally determined...
  37. pmc Biosynthesis of a natural polyketide-isoprenoid hybrid compound, furaquinocin A: identification and heterologous expression of the gene cluster
    Takashi Kawasaki
    Biotechnology Research Center, Toyama Prefectural University, Toyama 939 0398, Japan
    J Bacteriol 188:1236-44. 2006
    ....
  38. doi request reprint Studies on a new biosynthetic pathway for menaquinone
    Haruo Seto
    Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Am Chem Soc 130:5614-5. 2008
    ..Menaquinone is biosynthesized from chorismate via a new pathway involving 1,4-dihydroxy-6-naphthoate in Streptomyces and presumably in pathogenic bacteria Helicobacter and Campylobacter...