Research Topics
Species | Takaji WakitaSummaryAffiliation: Tokyo Metropolitan Institute for Neuroscience Country: Japan Publications
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Detail Information
Publications
HCV research and anti-HCV drug discovery: toward the next generationTakaji Wakita
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo 162 8640, Japan
Adv Drug Deliv Rev 59:1196-9. 2007..Such progress will propel HCV research and anti-HCV drug discovery toward the next generation...
Detection of anti-hepatitis C virus effects of interferon and ribavirin by a sensitive replicon systemTakanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan
J Clin Microbiol 43:5679-84. 2005..This system will provide a powerful tool for screening new antiviral compounds against HCV...
[Hepatitis C virus vaccine]Takaji Wakita
Department of Virology II, National Institute of Infectious Diseases
Nihon Rinsho 66:1961-4. 2008..A neutralizing antibody induction and a vaccine development are necessary for prophylaxis of HCV infection. Here we summarize the recent progress in basic and clinical research toward the vaccine development for HCV...
Replication of a hepatitis C virus replicon clone in mouse cellsSusan L Uprichard
The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, CA 92037 USA
Virol J 3:89. 2006..In effort to develop experimental HCV mouse models, we screened a panel of HCV replicons to identify clones capable of replicating in mouse hepatocytes...
Possible role of cytotoxic T cells in acute liver injury in hepatitis C virus cDNA transgenic mice mediated by Cre/loxP systemT Wakita
Department of Microbiology, The Tokyo Metropolitan Institute of Medical Science, Japan
J Med Virol 62:308-17. 2000..HCV-specific CTLs may be to enhance hepatitis. Thus, the present HCV transgenic mouse model provides a useful model of liver injury due to HCV, and the host immune response may play a pivotal role(s) in the pathogenesis of HCV...
Production of infectious hepatitis C virus in tissue culture from a cloned viral genomeTakaji Wakita
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183 8526, Japan
Nat Med 11:791-6. 2005..The cell culture-generated HCV is infectious for chimpanzee. This system provides a powerful tool for studying the viral life cycle and developing antiviral strategies...
Genotype 2a hepatitis C virus subgenomic replicon can replicate in HepG2 and IMY-N9 cellsTomoko Date
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183-8526, Japan
J Biol Chem 279:22371-6. 2004..This system with the JFH-1 replicon and three cell lines is useful not only for estimating the cellular factors affecting viral activity but also for clarifying the common gene response of the host...
Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA cloneZijiang Zhao
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan
J Gen Virol 86:2209-20. 2005..This study demonstrates clearly that a single Glu-to-Lys mutation at aa 138 of the envelope protein affects multiple steps of the viral life cycle. These multiple changes may induce substantial attenuation of JEV...
Comparison between subgenomic replicons of hepatitis C virus genotypes 2a (JFH-1) and 1b (Con1 NK5.1)Michiko Miyamoto
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu-shi, Tokyo 183-8526, Japan
Intervirology 49:37-43. 2006..Therefore, further investigation may be needed on more HCV isolates of diverse genotypes...
Nonhepatic cell lines HeLa and 293 support efficient replication of the hepatitis C virus genotype 2a subgenomic repliconTakanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
J Virol 79:592-6. 2005..Sequencing analysis revealed that mutations in HCV-derived regions were not essential for replication in these cells, as some clones displayed no mutations...
The roles of CD81 and glycosaminoglycans in the adsorption and uptake of infectious HCV particlesKenichi Morikawa
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
J Med Virol 79:714-23. 2007..HSPG may play a role in the initial cell surface binding of infectious HCV particles and CD81 is conceivably correlated with HCV entry after viral attachment...
An infectious and selectable full-length replicon system with hepatitis C virus JFH-1 strainTomoko Date
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
Hepatol Res 37:433-43. 2007..Conclusions: Selectable infection was established using HCV replicon containing full-length genotype 2a JFH-1 cDNA. This system might be useful for HCV research...
Efficient replication of the genotype 2a hepatitis C virus subgenomic repliconTakanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Japan
Gastroenterology 125:1808-17. 2003..This system provides a powerful new tool for researching HCV...
[Production of infectious hepatitis C virus in cell culture]Takanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo
Uirusu 55:287-95. 2005..Furthermore, secreted virus displayed infectivity for naive Huh7 cells. This system provides a powerful tool for studying the viral life cycle and constructing anti-viral strategies...
Repeated hepatocyte injury promotes hepatic tumorigenesis in hepatitis C virus transgenic miceTakanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Fucyu, Tokyo 183-8526, Japan
Cancer Sci 94:679-85. 2003..Furthermore, this experimental mouse model provides a valuable method with which to investigate hepatocarcinogenesis...
Processing of hepatitis C virus core protein is regulated by its C-terminal sequenceTakanobu Kato
Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
J Med Virol 69:357-66. 2003..The data suggest that core protein processing is regulated by C-terminus mutations...
Inoculation of plasmids encoding Japanese encephalitis virus PrM-E proteins with colloidal gold elicits a protective immune response in BALB/c miceZijiang Zhao
Department of Microbiology and Immunology, Tokyo Metropolitan Institute for Neuroscience, Fuchu-shi, Japan
J Virol 77:4248-60. 2003..DNA immunization with colloidal gold elicited encoded protein expression in splenocytes and might enhance immune responses in intravenously inoculated mice. This approach could be exploited to develop a novel DNA vaccine...
DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replicationYasuo Ariumi
Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
J Virol 81:13922-6. 2007..Thus, DDX3 is required for HCV RNA replication...
Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferonNobuhiko Hiraga
Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
FEBS Lett 581:1983-7. 2007..Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs...
Establishment of an infectious genotype 1b hepatitis C virus clone in human hepatocyte chimeric miceTakashi Kimura
Department of Medicine and Molecular Science, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
J Gen Virol 89:2108-13. 2008..This mouse model will be useful for the study of HCV virology, particularly the mechanism underlying the variable resistance of HCV genotypes to interferon therapy...
Development of plaque assays for hepatitis C virus-JFH1 strain and isolation of mutants with enhanced cytopathogenicity and replication capacityYuko Sekine-Osajima
Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Tokyo, Japan
Virology 371:71-85. 2008..Taken together, the cytopathic effect of HCV infection involves ER-stress-induced apoptotic cell death. Certain HCV genomic structures may determine the viral replication capacity and cytopathogenicity...
Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81Mirjam B Zeisel
Institut National de la Sante et de la Recherche Medicale INSERM, U748, Strasbourg, France
Hepatology 46:1722-31. 2007..Conclusion: Our data suggest that SR-BI (i) represents a key host factor for HCV entry, (ii) is implicated in the same HCV entry pathway as CD81, and (iii) targets an entry step closely linked to HCV-CD81 interaction...
Hepatitis C virus non-structural proteins responsible for suppression of the RIG-I/Cardif-induced interferon responseMegumi Tasaka
Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, Tokyo, Japan
J Gen Virol 88:3323-33. 2007..These mechanisms may contribute to the clinical persistence of HCV infection and could constitute a novel antiviral therapeutic target...
Critical role of virion-associated cholesterol and sphingolipid in hepatitis C virus infectionHideki Aizaki
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Virol 82:5715-24. 2008....
Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringeninYaakov Nahmias
Center for Engineering in Medicine, Shriners Burns Hospital, Boston, MA, USA
Hepatology 47:1437-45. 2008..CONCLUSION: These results suggest a novel therapeutic approach for the treatment of HCV infection...
Trans-encapsidation of hepatitis C virus subgenomic replicon RNA with viral structure proteinsKoji Ishii
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
Biochem Biophys Res Commun 371:446-50. 2008....
Intragenotypic JFH1 based recombinant hepatitis C virus produces high levels of infectious particles but causes increased cell deathGuaniri Mateu
Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30329, USA
Virology 376:397-407. 2008..These studies begin to unravel requirements for robust virus replication and the relationship between increased virion production and host cell viability...
Hepatitis C virus JFH-1 strain infection in chimpanzees is associated with low pathogenicity and emergence of an adaptive mutationTakanobu Kato
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Hepatology 48:732-40. 2008..CONCLUSION: Our study shows that the HCV JFH-1 strain causes attenuated infection and low pathogenicity in chimpanzees and is capable of adapting in vivo with a unique mutation conferring an enhanced replicative phenotype...
Induction of incomplete autophagic response by hepatitis C virus via the unfolded protein responseDonna Sir
Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA
Hepatology 48:1054-61. 2008..These unexpected effects of HCV on the host cell likely play an important role in HCV pathogenesis...
Cell culture and infection system for hepatitis C virusTakanobu Kato
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Protoc 1:2334-9. 2006..Viral particles can be obtained in 12 days with this protocol...
Robust hepatitis C virus infection in vitroJin Zhong
Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 102:9294-9. 2005..This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen...
Robust production of infectious hepatitis C virus (HCV) from stably HCV cDNA-transfected human hepatoma cellsZhaohui Cai
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, 800 Rose Street, Lexington, KY 40536-0298, USA
J Virol 79:13963-73. 2005..Collectively, these results demonstrate the establishment of a stable HCV culture system that robustly produces infectious virus, which will allow the study of each aspect of the entire HCV life cycle...
Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cellsMinkyung Yi
Center for Hepatitis Research, Institute for Human Infections and Immunity, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019, USA
Proc Natl Acad Sci U S A 103:2310-5. 2006..The ability of this genotype 1a virus to infect cultured cells will substantially benefit antiviral and vaccine discovery programs...
Diverse effects of cyclosporine on hepatitis C virus strain replicationNaoto Ishii
Laboratory of Human Tumor Viruses, Department of Viral Oncology, Institute for Virus Research, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
J Virol 80:4510-20. 2006..These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents...
Generation of infectious hepatitis C virus in immortalized human hepatocytesTatsuo Kanda
Department of Pathology, Saint Louis University, 3635 Vista Avenue, St. Louis, Missouri 63110, USA
J Virol 80:4633-9. 2006..A similar level of virus growth was observed upon transfection of RNA from HCV genotype 2a (JFH1) into IHH. Taken together, our results suggest that IHH support HCV genome replication and virus assembly...
Hepatitis C virus entry depends on clathrin-mediated endocytosisEmmanuelle Blanchard
, CNRS-UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
J Virol 80:6964-72. 2006..These data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment...
Production of infectious hepatitis C virus of various genotypes in cell culturesTakanobu Kato
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 10 Center Drive, Rm 9B16, Bethesda, MD 20892, USA
J Virol 81:4405-11. 2007..The development of culture systems for production of various HCV genotypes provides a valuable tool not only to study the replication and pathogenesis of HCV but also to screen for antivirals...
The lipid droplet is an important organelle for hepatitis C virus productionYusuke Miyanari
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
Nat Cell Biol 9:1089-97. 2007..This study reveals a novel function of LDs in the assembly of infectious HCV and provides a new perspective on how viruses usurp cellular functions...
Induction of hepatic injury by hepatitis C virus-specific CD8+ murine cytotoxic T lymphocytes in transgenic mice expressing the viral structural genesShun Takaku
Department of Microbiology and Immunology, Nippon Medical, School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
Biochem Biophys Res Commun 301:330-7. 2003..To our knowledge, this is the first demonstration to show that HCV-specific CD8(+) CTLs specifically attack hepatocytes expressing the HCV structural proteins both in vitro and in vivo...
Regulation of hepatitis C virus replication by interferon regulatory factor 1Nobuhiko Kanazawa
Department of Gastroenterology and Hepatology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
J Virol 78:9713-20. 2004..Taken together, IRF-1 is one of the key host factors that regulate intracellular HCV replication through modulation of interferon-stimulated-gene-mediated antiviral responses...
The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replicationYasuo Ariumi
Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1, Shikata cho, Okayama 700 8558, Japan
J Virol 82:9639-46. 2008..Taken together, these results suggest that the ATM signaling pathway is critical for HCV RNA replication and may represent a novel target for the clinical treatment of patients with chronic hepatitis C...
Virological characterization of the hepatitis C virus JFH-1 strain in lymphocytic cell linesKyoko Murakami
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Gen Virol 89:1587-92. 2008..Our results suggest that lymphocytic cells can support HCV JFH-1 translation and polyprotein processing, but may lack some host factors essential for HCV JFH-1 infection and replication...
CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infectionDaisuke Akazawa
Pharmaceutical Research Laboratories, Toray Industries, Inc, Kanagawa, Japan
J Virol 81:5036-45. 2007..In conclusion, CD81 expression is an important determinant of HCV permissiveness of Huh7 cell clones harboring different characteristics...
The NS3 helicase and NS5B-to-3'X regions are important for efficient hepatitis C virus strain JFH-1 replication in Huh7 cellsAsako Murayama
Department of Virology II, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku, Tokyo 162 8640, Japan
J Virol 81:8030-40. 2007..In conclusion, the JFH-1 NS3 helicase and NS5B-to-3'X regions are important for efficient replication and virus particle formation of HCV genotype 2a strains...
Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virusYves Rouille
CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
J Virol 80:2832-41. 2006..In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle...
Monitoring the antiviral effect of alpha interferon on individual cellsChon Saeng Kim
PBC, Department of Life Science, Pohang University of Science and Technology, San31, Hyoja Dong, Pohang 790 784, Republic of Korea
J Virol 81:8814-20. 2007..Responses of individual cells containing HCV to alpha interferon (IFN-alpha) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-alpha were observed among HCV-containing cells...
Molecular biology of hepatitis C virusTetsuro Suzuki
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Tokyo, Japan
J Gastroenterol 42:411-23. 2007..This review summarizes the current knowledge of cell culture systems for HCV research and recent advances in the investigation of the molecular virology of HCV...
Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteinsDavid Delgrange
CNRS UMR 8161, IBL, Université de Lille I et Lille II, Institut Pasteur de Lille, 59021 Lille Cedex, France
J Gen Virol 88:2495-503. 2007..Altogether, our data indicate that a more robust production of HCVcc particles can be obtained by introducing a few specific mutations in JFH-1 structural proteins...
Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infectionJessica Wagoner
Department of Laboratory Medicine, University of Washington, Virology 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
J Virol 81:309-18. 2007....
E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis C virus core proteinMasayuki Shirakura
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
J Virol 81:1174-85. 2007..We propose that the E6AP-mediated ubiquitin-proteasome pathway may affect the production of HCV particles through controlling the amounts of viral nucleocapsid protein...
Upregulation of indoleamine 2,3-dioxygenase in hepatitis C virus infectionEsther Larrea
Division of Gene Therapy and Hepatology, Center for Applied Medical Research, CIMA, Avenida Pio XII 55, 31008 Pamplona, Spain
J Virol 81:3662-6. 2007..For both patients and chimpanzees, hepatic expression of IDO and CTLA-4 correlated directly. Induction of IDO may dampen T-cell reactivity to viral antigens in chronic HCV infection...
Hepatitis C viral life cycleTetsuro Suzuki
Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
Adv Drug Deliv Rev 59:1200-12. 2007..This review summarizes our current understanding of genomic organization of HCV, features of the viral protein characteristics, and the viral life cycle...
Hepatitis C virus-infected hepatocytes extrinsically modulate dendritic cell maturation to activate T cells and natural killer cellsTakashi Ebihara
Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
Hepatology 48:48-58. 2008....
