S Uchida

Summary

Affiliation: Tokyo Medical and Dental University
Country: Japan

Publications

  1. pmc A young child with pseudohypoaldosteronism type II by a mutation of Cullin 3
    Shoji Tsuji
    Department of Pediatrics, Kansai Medical University, 2 5 1 Shin machi, Hirakata shi, Osaka 573 1010, Japan
    BMC Nephrol 14:166. 2013
  2. pmc Baseline characteristics and prevalence of cardiovascular disease in newly visiting or referred chronic kidney disease patients to nephrology centers in Japan: a prospective cohort study
    Soichiro Iimori
    Department of Nephrology, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo ku, Tokyo 113 8519, Japan
    BMC Nephrol 14:152. 2013
  3. ncbi request reprint Isolation of a novel zinc finger repressor that regulates the kidney-specific CLC-K1 promoter
    S Uchida
    Homeostasis Medicine and Nephrology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Kidney Int 60:416-21. 2001
  4. doi request reprint Pathophysiological roles of WNK kinases in the kidney
    Shinichi Uchida
    Department of Nephrology, Tokyo Medical and Dental University, Yushima, Bunkyo ku, Tokyo, Japan
    Pflugers Arch 460:695-702. 2010
  5. ncbi request reprint Function of chloride channels in the kidney
    Shinichi Uchida
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Annu Rev Physiol 67:759-78. 2005
  6. pmc Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2
    Shinichi Uchida
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Mol Cell Biol 25:7357-63. 2005
  7. pmc Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Krüppel-like factor, a novel zinc finger repressor
    S Uchida
    Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Mol Cell Biol 20:7319-31. 2000
  8. ncbi request reprint Cloning and expression of apical membrane water channel of rat kidney collecting tubule
    K Fushimi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Nature 361:549-52. 1993
  9. pmc Localization and functional characterization of rat kidney-specific chloride channel, ClC-K1
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    J Clin Invest 95:104-13. 1995
  10. ncbi request reprint Cloning, tissue distribution, and intrarenal localization of ClC chloride channels in human kidney
    Y Takeuchi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Kidney Int 48:1497-503. 1995

Collaborators

Detail Information

Publications87

  1. pmc A young child with pseudohypoaldosteronism type II by a mutation of Cullin 3
    Shoji Tsuji
    Department of Pediatrics, Kansai Medical University, 2 5 1 Shin machi, Hirakata shi, Osaka 573 1010, Japan
    BMC Nephrol 14:166. 2013
    ..Here, we firstly report on the Japanese child of PHA II caused by a mutation of CUL 3...
  2. pmc Baseline characteristics and prevalence of cardiovascular disease in newly visiting or referred chronic kidney disease patients to nephrology centers in Japan: a prospective cohort study
    Soichiro Iimori
    Department of Nephrology, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo ku, Tokyo 113 8519, Japan
    BMC Nephrol 14:152. 2013
    ..In this report, we describe the baseline characteristics and risk factors for cardiovascular disease (CVD) prevalence among this cohort...
  3. ncbi request reprint Isolation of a novel zinc finger repressor that regulates the kidney-specific CLC-K1 promoter
    S Uchida
    Homeostasis Medicine and Nephrology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Kidney Int 60:416-21. 2001
    ..These results suggest that a novel set of zinc finger proteins may help regulate the strict tissue and nephron segment-specific expression of CLC-K1 and CLC-K2 channel genes through their GA cis element...
  4. doi request reprint Pathophysiological roles of WNK kinases in the kidney
    Shinichi Uchida
    Department of Nephrology, Tokyo Medical and Dental University, Yushima, Bunkyo ku, Tokyo, Japan
    Pflugers Arch 460:695-702. 2010
    ....
  5. ncbi request reprint Function of chloride channels in the kidney
    Shinichi Uchida
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Annu Rev Physiol 67:759-78. 2005
    ....
  6. pmc Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2
    Shinichi Uchida
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Mol Cell Biol 25:7357-63. 2005
    ..Thus, UT-A2 is important for maintaining a high concentration of urea in the inner medulla when urea supply to the kidney is limited...
  7. pmc Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Krüppel-like factor, a novel zinc finger repressor
    S Uchida
    Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Mol Cell Biol 20:7319-31. 2000
    ..These results suggest that a novel set of zinc finger proteins may help regulate the strict tissue- and nephron segment-specific expression of the CLC-K1 and CLC-K2 channel genes through their GA cis element...
  8. ncbi request reprint Cloning and expression of apical membrane water channel of rat kidney collecting tubule
    K Fushimi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Nature 361:549-52. 1993
    ..The functional expression and the limited localization of WCH-CD to the apical region of the kidney collecting tubule suggest that WCH-CD is the vasopressin-regulated water channel...
  9. pmc Localization and functional characterization of rat kidney-specific chloride channel, ClC-K1
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    J Clin Invest 95:104-13. 1995
    ..The localization and the functional characteristics described here indicate that ClC-K1 is responsible for the transepithelial chloride transport in tAL...
  10. ncbi request reprint Cloning, tissue distribution, and intrarenal localization of ClC chloride channels in human kidney
    Y Takeuchi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Kidney Int 48:1497-503. 1995
    ..Identification of human ClC-K2 clone will be of help in understanding the genetic involvement of chloride channel in disorders of chloride transport such as Bartter's syndrome...
  11. pmc Expression of vascular endothelial growth factor, matrix metalloproteinase-9 and E-cadherin in the process of lymph node metastasis in oesophageal cancer
    F Sato
    Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 80:1366-72. 1999
    ..These data suggest that the expression of VEGF, MMP-9 and E-cadherin each change in the process of lymph node metastasis in oesophageal cancer, and that the patterns of change are different...
  12. ncbi request reprint Identification of a new outwardly rectifying Cl- channel that belongs to a subfamily of the ClC Cl- channels
    H Sakamoto
    Second Department of Internal Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113, Japan
    J Biol Chem 271:10210-6. 1996
    ..The molecular structure and functional properties of ClC-5 will provide an important insight into ORCCs and the pathogenesis of Dent's disease...
  13. pmc Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells
    K Ishibashi
    School of Medicine, Tokyo Medical and Dental University, Japan
    Proc Natl Acad Sci U S A 91:6269-73. 1994
    ..In kidney, it was exclusively immunolocalized at the basolateral membrane of collecting duct cells. AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells...
  14. ncbi request reprint Isolation and characterization of the human CLC-5 chloride channel gene promoter
    A Hayama
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, 1 5 45 Yushima Bunkyo ku, 113 8519, Tokyo, Japan
    Gene 261:355-64. 2000
    ..This study clarified the key element of the human CLCN5 promoter, and the mutation in this region could be the cause of Dent's disease...
  15. ncbi request reprint Tissue expression of mRNA of chloride channel from MDCK cells and its regulation by protein kinases
    K Ishibashi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Biochem Biophys Res Commun 192:561-7. 1993
    ..Both phorbol myristate acetate (PMA) and forskolin down regulated its expression in MDCK cells, suggesting that both PKC and PKA modulated its expression. The physiological role of this chloride channel remains to be clarified...
  16. ncbi request reprint Cellular and subcellular immunolocalization of ClC-5 channel in mouse kidney: colocalization with H+-ATPase
    H Sakamoto
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Am J Physiol 277:F957-65. 1999
    ..These findings suggest that ClC-5 might be involved in the endocytosis and/or the H+ secretion in the proximal tubule cells and the cortical collecting duct type A intercalated cells in mouse kidney...
  17. ncbi request reprint Regulation of aquaporin-2 gene transcription by GATA-3. off
    S Uchida
    Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan
    Biochem Biophys Res Commun 232:65-8. 1997
    ..These results indicated that GATA motifs in the 5'-flanking region of the hAQP-2 gene were functional cis-elements and that GATA-3 in collecting ducts may be one of the important regulators of AQP-2 expression in vivo...
  18. ncbi request reprint Intrarenal and cellular localization of CLC-K2 protein in the mouse kidney
    K Kobayashi
    Department of Pediatrics, Tokyo Medical and Dental University, School of Medicine, 1 5 45 Yushima, Bunkyo ku, Tokyo, 113, Japan
    J Am Soc Nephrol 12:1327-34. 2001
    ..These aspects of CLC-K2 localization suggest that CLC-K2 is important in Cl(-) transport in the distal nephron segments...
  19. ncbi request reprint Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    J Biol Chem 268:3821-4. 1993
    ..The site of expression and the regulation by dehydration suggest that CIC-K1 function may be important in urinary concentrating mechanisms...
  20. ncbi request reprint Developmental expression of CLC-K1 in the postnatal rat kidney
    K Kobayashi
    Department of Pediatrics, Tokyo Medical and Dental University, School of Medicine, 1 5 45 Yushima, Bunkyo ku, Tokyo, 113 8519, Japan
    Histochem Cell Biol 116:49-56. 2001
    ..These results suggest that CLC-K1 expression is one of the major determinants of the urine-concentrating capacity of the developing rat kidney...
  21. ncbi request reprint Two isoforms of a chloride channel predominantly expressed in thick ascending limb of Henle's loop and collecting ducts of rat kidney
    S Adachi
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    J Biol Chem 269:17677-83. 1994
    ..These results suggest that ClC-K2L and -K2S are chloride channels in the thick ascending limb and collecting ducts and may be important routes for transcellular chloride transport like ClC-K1...
  22. ncbi request reprint Impaired solute accumulation in inner medulla of Clcnk1-/- mice kidney
    N Akizuki
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Am J Physiol Renal Physiol 280:F79-87. 2001
    ..We concluded that NDI in Clcnk1-/- mice resulted from an impairment in the generation of inner medullary hypertonicity by a dysfunction of the countercurrent systems...
  23. pmc Three families with autosomal dominant nephrogenic diabetes insipidus caused by aquaporin-2 mutations in the C-terminus
    M Kuwahara
    Department of Homeostasis Medicine and Nephrology, Graduate School, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Am J Hum Genet 69:738-48. 2001
    ..Segregation of the mutations in the C-terminus of AQP2 with dominant-type NDI underlies the importance of this domain in the intracellular trafficking of AQP2...
  24. ncbi request reprint Cloning of rat and mouse aquaporin-2 gene promoters and identification of a negative cis-regulatory element
    T Rai
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    Am J Physiol 273:F264-73. 1997
    ..These results indicate that tissue-specific expression of AQP-2 gene may in part be regulated by this novel negative acting cis-element...
  25. ncbi request reprint Severely impaired urine-concentrating ability in mice lacking the CLC-K1 chloride channel
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Tokyo, Japan
    Exp Nephrol 8:361-5. 2000
    ..These results clearly established that CLC-K1 plays a major role in the urinary-concentrating mechanisms...
  26. ncbi request reprint Isolation of human aquaporin-CD gene
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    J Biol Chem 269:23451-5. 1994
    ....
  27. ncbi request reprint Assignment of the genes encoding the human chloride channels, CLCNKA and CLCNKB, to 1p36 and of CLCN3 to 4q32-q33 by in situ hybridization
    F Saito-Ohara
    Department of Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo, 113, Japan
    Genomics 36:372-4. 1996
  28. ncbi request reprint Two sporadic cases of Liddle's syndrome caused by De novo ENaC mutations
    Y Yamashita
    Homeostasis Medicine and Nephrology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Am J Kidney Dis 37:499-504. 2001
    ..These data indicate that Liddle's syndrome should be considered even in patients without a family history of hypertension...
  29. ncbi request reprint Localization of mouse CLC-6 and CLC-7 mRNA and their functional complementation of yeast CLC gene mutant
    Y Kida
    Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan
    Histochem Cell Biol 115:189-94. 2001
    ..This study identified the cell types that express mCLC-6 and mCLC-7 in the mouse tissues, and the complementation assay suggested that mCLC-6 functions as an intracellular chloride channel...
  30. ncbi request reprint Isolation of human aquaporin 3 gene
    N Inase
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    J Biol Chem 270:17913-6. 1995
    ..These findings about the genomic clone of human AQP3 will contribute to elucidate the molecular mechanism of transcriptional regulation of AQP3...
  31. ncbi request reprint In vivo role of CLC chloride channels in the kidney
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Tokyo 113 8519, Japan
    Am J Physiol Renal Physiol 279:F802-8. 2000
    ..e., their intrarenal and cellular localization and their phenotypes of humans and mice that have their loss-of-function mutations...
  32. pmc Motility-related protein (MRP-1/CD9) and KAI1/CD82 expression inversely correlate with lymph node metastasis in oesophageal squamous cell carcinoma
    S Uchida
    Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 79:1168-73. 1999
    ..Knowing the status of the expression of MRP-1/CD9 appears helpful in predicting the prognosis for each patient...
  33. ncbi request reprint Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1 chloride channel
    Y Matsumura
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    Nat Genet 21:95-8. 1999
    ..These results establish that CLC-K1 has a role in urine concentration, and that the countercurrent system in the inner medulla is involved in the generation and maintenance of hypertonic medullary interstitium...
  34. pmc Disease-causing mutant WNK4 increases paracellular chloride permeability and phosphorylates claudins
    Kozue Yamauchi
    Department of Nephrology, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 101:4690-4. 2004
    ..The increased paracellular "chloride shunt" caused by the mutant WNK4 could be the pathogenic mechanism of PHAII...
  35. ncbi request reprint Defective water and glycerol transport in the proximal tubules of AQP7 knockout mice
    Eisei Sohara
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Am J Physiol Renal Physiol 289:F1195-200. 2005
    ..007 +/- 0.005 mg/ml; cisplatin, 0.063 +/- 0.043 mg/ml; ischemia, 0.076 +/- 0.02 mg/ml), suggesting that urine glycerol could be used as a new biomarker for detecting proximal straight tubule injury...
  36. ncbi request reprint Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells
    M Kawasaki
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    Neuron 12:597-604. 1994
    ..These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C...
  37. ncbi request reprint Tight regulation of transgene expression by tetracycline-dependent activator and repressor in brain
    S Uchida
    Department of Agricultural Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    Genes Brain Behav 5:96-106. 2006
    ..These results indicate that this system allows the tight regulation of transgene expression in a Dox-dependent fashion in the forebrain and will be useful in investigating gene function in the brain...
  38. ncbi request reprint Chloride transport across kidney epithelia through CLC chloride channels
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Nihon Jinzo Gakkai Shi 38:285-9. 1996
    ..The functional properties and intrarenal localization of these chloride channels are summarized, and their involvement in certain tubular dysfunctions and physiological roles are discussed in this report...
  39. pmc In oesophageal squamous cell carcinoma vascular endothelial growth factor is associated with p53 mutation, advanced stage and poor prognosis
    S Uchida
    Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 77:1704-9. 1998
    ..0077). We have assumed that VEGF contributes to aggressive characteristics in oesophageal carcinomas and that VEGF expression might be affected by p53 status...
  40. pmc Prognostic factors of oesophageal squamous cell carcinoma from the perspective of molecular biology
    Y Shimada
    Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 80:1281-8. 1999
    ..18). Our data indicate that combination analysis using pN, sex, cyclin D1, E-cadherin, EGFR and cell regrowth capability may be useful for the prediction of patient survival and recurrence...
  41. ncbi request reprint Molecular cloning of CLC chloride channels in Oreochromis mossambicus and their functional complementation of yeast CLC gene mutant
    H Miyazaki
    Second Department of Internal Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Tokyo, Bunkyo, 113 8519, Japan
    Biochem Biophys Res Commun 255:175-81. 1999
    ..These results clearly indicated that CLC channels closely related to the mammalian CLC-3, -4, and -5 subfamily exist also in tilapia and that OmCLC-3 and OmCLC-5 function as intracellular chloride channels...
  42. ncbi request reprint Physiological role of CLC-K1 chloride channel in the kidney
    S Uchida
    Second Department of Internal Medicine, Tokyo Medical and Dental University, School of Medicine, Japan
    Nephrol Dial Transplant 15:14-5. 2000
    ..Exact clearance studies and measurements of osmolality and solute contents in the inner medulla will provide the answer to this question...
  43. ncbi request reprint Progression of esophageal carcinoma by loss of EGF-STAT1 pathway
    G Watanabe
    Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan
    Cancer J 7:132-9. 2001
    ..This loss appears to cause a significantly more malignant clinical course. These findings may point out a critical step in the progression of esophageal cancer and could lead to the development of useful clinical applications...
  44. pmc Molecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(-)-dependent taurine transporter that is regulated by hypertonicity
    S Uchida
    2nd Department of Internal Medicine, Tokyo Medical and Dental University, Japan
    Proc Natl Acad Sci U S A 89:8230-4. 1992
    ..The abundance of mRNA for the taurine transporter is increased in MDCK cells cultured in hypertonic medium, suggesting that regulation of transport activity by medium hypertonicity occurs at the level of mRNA accumulation...
  45. ncbi request reprint Apical localization of renal K channel was not altered in mutant WNK4 transgenic mice
    Kozue Yamauchi
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Biochem Biophys Res Commun 332:750-5. 2005
    ..This result indicates that the mutant WNK4 does not have a dominant effect on the cellular localization of ROMK in vivo...
  46. doi request reprint FAPP2 is required for aquaporin-2 apical sorting at trans-Golgi network in polarized MDCK cells
    Naofumi Yui
    Dept of Nephrology, Tokyo Medical and Dental University, Japan
    Am J Physiol Cell Physiol 297:C1389-96. 2009
    ..These results suggest that FAPP2 is necessary to generate AQP2-bearing vesicles at trans-Golgi that will undergo phosphorylation by PKA in subapical regions...
  47. ncbi request reprint Postnatal expression of KLF12 in the inner medullary collecting ducts of kidney and its trans-activation of UT-A1 urea transporter promoter
    Shin Suda
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan
    Biochem Biophys Res Commun 344:246-52. 2006
    ..These results suggest that KLF12 is involved in the maturation processes of collecting ducts after birth, and that UT-A1 is a target gene of KLF12...
  48. ncbi request reprint Polarized trafficking of the aquaporin-3 water channel is mediated by an NH2-terminal sorting signal
    Tatemitsu Rai
    Dept of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental Univ, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Am J Physiol Cell Physiol 290:C298-304. 2006
    ..On the basis of these results, we conclude that an NH2-terminal sorting signal mediates the basolateral targeting of AQP3...
  49. ncbi request reprint Physiological roles of AQP7 in the kidney: Lessons from AQP7 knockout mice
    Eisei Sohara
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, Tokyo 113 8519, Japan
    Biochim Biophys Acta 1758:1106-10. 2006
    ..007+/-0.005 mg/ml; cisplatin, 0.063+/-0.043 mg/ml; ischemia, 0.076+/-0.02 mg/ml), suggesting that urine glycerol could be used as a new biomarker for detecting proximal straight tubule injury...
  50. pmc Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct
    S Sasaki
    Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan
    J Clin Invest 93:1250-6. 1994
    ..Chromosomal mapping of the hAQP-CD gene assigned its location to chromosome 12q13. These results could be important for future studies of the pathophysiology of human urinary concentration mechanisms in normal and abnormal states...
  51. ncbi request reprint Overexpression of human WNK1 increases paracellular chloride permeability and phosphorylation of claudin-4 in MDCKII cells
    Akihito Ohta
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Biochem Biophys Res Commun 349:804-8. 2006
    ..Thus, increased chloride shunt may be involved in the pathogenesis of PHAII caused by WNK1 mutations...
  52. ncbi request reprint [Molecular mechanism in substance transport in the kidney: Chloride channel]
    Shinichi Uchida
    Department of Nephrology, Tokyo Medical and Dental University
    Nihon Rinsho 64:129-33. 2006
  53. doi request reprint Targeted disruption of the Wnk4 gene decreases phosphorylation of Na-Cl cotransporter, increases Na excretion and lowers blood pressure
    Akihito Ohta
    Department of Nephrology, Tokyo Medical and Dental University, Japan
    Hum Mol Genet 18:3978-86. 2009
    ..Thus, wild-type WNK4 is a positive regulator for the WNK-OSR1/SPAK-NCC cascade, and WNK4 is a potential target of anti-hypertensive drugs...
  54. ncbi request reprint Calcitonin gene-related peptide produces skeletal muscle vasodilation following antidromic stimulation of unmyelinated afferents in the dorsal root in rats
    A Sato
    Graduate School of Humanities and Sciences, Ochanomizu University, 2 1 1, Otsuka, Bunkyo ku, Tokyo, Japan
    Neurosci Lett 283:137-40. 2000
    ..It is suggested that this CGRP-related antidromic vasodilation may be important in the clinical improvement of skeletal MBF produced by physical therapy, e.g. acupuncture...
  55. pmc Pathogenesis and treatment of autosomal-dominant nephrogenic diabetes insipidus caused by an aquaporin 2 mutation
    Eisei Sohara
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 103:14217-22. 2006
    ..Phosphodiesterase inhibitors may thus be useful drugs for the treatment of AD-NDI. This animal model demonstrates that a mutant monomer gains a dominant-negative effect that reverses the normal polarized sorting of multimers...
  56. ncbi request reprint CLC-3 deficiency leads to phenotypes similar to human neuronal ceroid lipofuscinosis
    Momono Yoshikawa
    Homeostasis Medicine and Nephrology, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo Tokyo 113 8519, Japan
    Genes Cells 7:597-605. 2002
    ..CLC-3 is a member of the CLC chloride channel family and is widely expressed in mammalian tissues. To determine the physiological role of CLC-3, we generated CLC-3-deficient mice (Clcn3-/- ) by targeted gene disruption...
  57. ncbi request reprint Human CLC-KB gene promoter drives the EGFP expression in the specific distal nephron segments and inner ear
    Katsuki Kobayashi
    Departments of Pediatrics, Homeostasis Medicine and Nephrology, and Audio Vestibular Neuroscience, Tokyo Medical and Dental University, Tokyo, Japan
    J Am Soc Nephrol 13:1992-8. 2002
    ....
  58. doi request reprint Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney
    Gulibaha Talati
    Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, Tokyo 113 8519, Japan
    Biochem Biophys Res Commun 393:844-8. 2010
    ..Although angiotensin II is identified as an upstream regulator for the WNK-OSR1/SPAK-NCC cascade in vivo, aldosterone appears to be the major regulator of this signal cascade in the long-term regulation by angiotensin II...
  59. ncbi request reprint Expression of CLC-KB gene promoter in the mouse cochlea
    Hirofumi Maehara
    Department of Otorhinolaryngology, Tokyo Medical and Dental University, Japan
    Neuroreport 14:1571-3. 2003
    ..Moreover, these fibrocytes may recycle Cl- through CLC that accompany Na+ and K+ into the cell via NKCC...
  60. ncbi request reprint Regulation of apical localization of the thiazide-sensitive NaCl cotransporter by WNK4 in polarized epithelial cells
    Sung Sen Yang
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, Tokyo 113 8519, Japan
    Biochem Biophys Res Commun 330:410-4. 2005
    ..The regulation of intracellular localization of NCC by WNK4 might not be involved in the pathogenesis of PHAII...
  61. ncbi request reprint Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase
    T Yamauchi
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Nat Med 8:1288-95. 2002
    ..Our data may provide a novel paradigm that an adipocyte-derived antidiabetic hormone, Ad, activates AMPK, thereby directly regulating glucose metabolism and insulin sensitivity in vitro and in vivo...
  62. ncbi request reprint Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice
    Eisei Sohara
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Fertil Steril 87:671-6. 2007
    ..To investigate the functional and morphologic role of Aquaporin 7 (AQP7) in testis and sperm...
  63. ncbi request reprint Barttin binds to the outer lateral surface of the ClC-K2 chloride channel
    Masato Tajima
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Biochem Biophys Res Commun 362:858-64. 2007
    ..This information regarding the binding sites will be useful for designing a new class of diuretics or anti-hypertensive agents that inhibit the interaction of ClC-K and barttin...
  64. ncbi request reprint Intracellular localization of ClC chloride channels and their ability to form hetero-oligomers
    Tatsunori Suzuki
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo Tokyo, Japan
    J Cell Physiol 206:792-8. 2006
    ..These results suggest that within cells ClC-3, ClC-4, and ClC-5 may have combinatorial functions, whereas ClC-6 and ClC-7 are more likely to function as homo-oligomers...
  65. ncbi request reprint Intracellular mislocalization of mutant podocin and correction by chemical chaperones
    Teiko Ohashi
    Department of Medicine, Kidney Center, Tokyo Women s Medical University, 8 1 Kawada cho Shinjuku, 162 8666, Tokyo, Japan
    Histochem Cell Biol 119:257-64. 2003
    ..Our results reveal defective cellular processing of the mutant podocin, and provide evidence for pharmacological correction of the processing defect...
  66. ncbi request reprint Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells
    Tomohiro Itoh
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, Tokyo 113 8519, Japan
    Biochem Biophys Res Commun 330:832-8. 2005
    ..The selective localization of AQP12 in pancreatic acinar cells and possibly in the intracellular organelles suggests a role of AQP12 in digestive enzyme secretion such as maturation and exocytosis of secretory granules...
  67. ncbi request reprint Molecular mechanisms of Bartter syndrome caused by mutations in the BSND gene
    Atsushi Hayama
    Department of Nephrology, Graduate School, School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima Bunkyo, 113 8519 Tokyo, Japan
    Histochem Cell Biol 119:485-93. 2003
    ..These results clarified that barttin determined cellular localization of CLC-K2 by protein-protein interaction. Thus, the mislocalization of CLC-K2 was identified as the molecular pathogenesis of Bartter syndrome by mutant barttins...
  68. doi request reprint Generation and analyses of R8L barttin knockin mouse
    Naohiro Nomura
    Department of Nephrology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan
    Am J Physiol Renal Physiol 301:F297-307. 2011
    ..These results suggest that the major cause of Bartter syndrome type IV caused by R8L barttin mutation is its aberrant intracellular localization...
  69. ncbi request reprint Molecular pathogenesis of pseudohypoaldosteronism type II: generation and analysis of a Wnk4(D561A/+) knockin mouse model
    Sung Sen Yang
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo, Tokyo 113 8519, Japan
    Cell Metab 5:331-44. 2007
    ..Thus, the pathogenesis of PHAII caused by a missense mutation of WNK4 was identified to be increased function of NCC through activation of the OSR1/SPAK-NCC phosphorylation cascade...
  70. doi request reprint Dietary salt regulates the phosphorylation of OSR1/SPAK kinases and the sodium chloride cotransporter through aldosterone
    Motoko Chiga
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Kidney Int 74:1403-9. 2008
    ..These studies suggest that that the WNK-OSR1/SPAK-NCC cascade may be a novel effector system of aldosterone action in the kidney...
  71. doi request reprint AKAP220 colocalizes with AQP2 in the inner medullary collecting ducts
    Rie Okutsu
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    Kidney Int 74:1429-33. 2008
    ..Forskolin-mediated phosphorylation of AQP2, transiently expressed in COS cells, was increased by AKAP220 co-expression. Our results suggest that AKAP220 may be involved in the phosphorylation of AQP2 by recruiting protein kinase A...
  72. doi request reprint A familial case of mitochondrial disease resembling Alport syndrome
    Hayahiko Fujii
    Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Clin Exp Nephrol 12:159-63. 2008
    ..This syndrome should be recognized by nephrologists as a differential diagnosis of Alport syndrome, diabetic nephropathy, and primary glomerular diseases...
  73. ncbi request reprint [Function of vasopressin in kidney]
    Shin Suda
    Department of Nephrology, Tokyo Medical and Dental University
    Nihon Rinsho 63:479-83. 2005
  74. pmc Kidney-specific chloride channel, OmClC-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney
    Hiroaki Miyazaki
    Ocean Research Institute, University of Tokyo, 1 15 1 Minamidai, Nakano Ku, Japan
    Proc Natl Acad Sci U S A 99:15782-7. 2002
    ..The localization of OmClC-K and its induction in hypoosmotic media suggest that OmClC-K is involved in Cl(-) reabsorption in the distal tubule of freshwater-adapted tilapia...
  75. ncbi request reprint Analysis of NaCl transport in thin ascending limb of Henle's loop in CLC-K1 null mice
    Wen Liu
    Department of Pediatrics, Tohoku University School of Medicine, Sendai 980 8574, Japan
    Am J Physiol Renal Physiol 282:F451-7. 2002
    ..These results clearly indicate that the major component of Cl(-) transport sensitive to NPPB or pH is mediated by CLC-K1 in the tAL...
  76. doi request reprint Intracellular ClC-3 chloride channels promote bone resorption in vitro through organelle acidification in mouse osteoclasts
    Fujio Okamoto
    Dept of Physiological Science and Molecular Biology, Fukuoka Dental College, 2 15 1 Tamura, Sawara ku, Fukuoka 814 0193, Japan
    Am J Physiol Cell Physiol 294:C693-701. 2008
    ..In conclusion, ClC-3 Cl(-) channels are expressed in intracellular organelles of mouse osteoclasts and contribute to osteoclastic bone resorption in vitro through organelle acidification...
  77. ncbi request reprint WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1
    Tetsuo Moriguchi
    Department of Molecular Cell Biology, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, and CREST, JST, Chiyoda, Tokyo 101 0062
    J Biol Chem 280:42685-93. 2005
    ..These results suggested that WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to the transporters and may provide insights into the mechanisms by which WNK1 regulates ion balance...
  78. ncbi request reprint ClC-3-independent, PKC-dependent activity of volume-sensitive Cl channel in mouse ventricular cardiomyocytes
    Weiqin Gong
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Cell Physiol Biochem 14:213-24. 2004
    ..We thus conclude that functional expression of VSOR Cl- channels in the plasma membrane of mouse cardiomyocytes is independent of the molecular expression of ClC-3...
  79. ncbi request reprint Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation
    Mariko Hara-Chikuma
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:15493-6. 2005
    ..Increasing AQP7 expression/function in adipocytes may reduce adipocyte volume and fat mass in obesity...
  80. ncbi request reprint Altered expression profile of transporters in the inner medullary collecting duct of aquaporin-1 knockout mice
    Ryan G Morris
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1603, USA
    Am J Physiol Renal Physiol 289:F194-9. 2005
    ..WT controls. In conclusion, the expression profile of IMCD transporters is markedly altered in AQP1 -/- mice and this manifestation is related to the associated concentrating defect...
  81. ncbi request reprint Hypertonicity regulates the aquaporin-2 promoter independently of arginine vasopressin
    Keizo Kasono
    Department of Medicine, Jichi Medical School, Omiya Medical Center, 1 847 Amanuma, Omiya ku, Saitama, Saitama 330 8503, Japan
    Nephrol Dial Transplant 20:509-15. 2005
    ..The present study was undertaken to determine whether a change in tonicity could directly regulate the AQP-2 gene in an in vitro experiment...
  82. ncbi request reprint ClC-3 chloride channels facilitate endosomal acidification and chloride accumulation
    Mariko Hara-Chikuma
    Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 280:1241-7. 2005
    ..3 versus 5.9. Our results provide direct evidence for involvement of ClC-3 in endosomal acidification by Cl- shunting of the interior-positive membrane potential created by the vacuolar H+ pump...
  83. ncbi request reprint Single-channel properties of volume-sensitive Cl- channel in ClC-3-deficient cardiomyocytes
    Jun Wang
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444 8585, Japan
    Jpn J Physiol 55:379-83. 2005
    ..These results indicate that the single-channel activity of the VSOR Cl(-) channel is independent of the expression of ClC-3 proteins in mouse ventricular myocytes...
  84. ncbi request reprint A case of a novel mutant vasopressin receptor-dependent nephrogenic diabetes insipidus with bilateral non-obstructive hydronephrosis in a middle aged man: differentiation from aquaporin-dependent nephrogenic diabetes insipidus by response of factor VII an
    Masashi Miyakoshi
    The Department of Internal Medicine, Division of Endocrine and Metabolism, Nagaoka Red Cross Hospital, Niigata, Japan
    Endocr J 50:809-14. 2003
    ..It was also found that the patient's mother and his second daughter were heterozygous for this R104C mutation...
  85. ncbi request reprint [Water channels]
    Shinichi Uchida
    Nihon Naika Gakkai Zasshi 95:873-6. 2006
  86. ncbi request reprint ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes
    Nobuyuki Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Cell Physiol Biochem 15:263-70. 2005
    ..Thus, we conclude that in cardiomyocytes, apoptosis is critically dependent on operation not of Cl-/HCO3- exchangers but of Cl- channels which are distinct from ClC-3...
  87. ncbi request reprint Water channel activities of Mimosa pudica plasma membrane intrinsic proteins are regulated by direct interaction and phosphorylation
    Yusuke Temmei
    Department of Chemistry, Faculty of Science and Technology, Sophia University, 7 1 Kioi, Chiyoda ku, Tokyo 102 8554, Japan
    FEBS Lett 579:4417-22. 2005
    ....