Mitsutoshi Tsukimoto

Summary

Affiliation: Tokyo University of Science
Country: Japan

Publications

  1. pmc TGF-β1 Downregulates COX-2 Expression Leading to Decrease of PGE2 Production in Human Lung Cancer A549 Cells, Which Is Involved in Fibrotic Response to TGF-β1
    Erina Takai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda shi, Chiba, Japan
    PLoS ONE 8:e76346. 2013
  2. pmc Involvement of connexin43 hemichannel in ATP release after γ-irradiation
    Yasuhiro Ohshima
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    J Radiat Res 53:551-7. 2012
  3. pmc Autocrine regulation of macrophage activation via exocytosis of ATP and activation of P2Y11 receptor
    Hayato Sakaki
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda shi Chiba, Japan
    PLoS ONE 8:e59778. 2013
  4. ncbi request reprint Low-dose gamma-ray irradiation induces translocation of Nrf2 into nuclear in mouse macrophage RAW264.7 cells
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, Chiba, Japan
    J Radiat Res 51:349-53. 2010
  5. doi request reprint Involvement of purinergic signaling in cellular response to gamma radiation
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan
    Radiat Res 173:298-309. 2010
  6. doi request reprint Blockade of murine T cell activation by antagonists of P2Y6 and P2X7 receptors
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Biochem Biophys Res Commun 384:512-8. 2009
  7. ncbi request reprint Repeated 0.5-Gy gamma irradiation attenuates experimental autoimmune encephalomyelitis with up-regulation of regulatory T cells and suppression of IL17 production
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Radiat Res 170:429-36. 2008
  8. doi request reprint 0.5 Gy gamma radiation suppresses production of TNF-alpha through up-regulation of MKP-1 in mouse macrophage RAW264.7 cells
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Radiat Res 171:219-24. 2009
  9. ncbi request reprint Diesel exhaust (DE) aggravates pathology of delayed-type hypersensitivity (DTH) induced by methyl-bovine serum albumin (mBSA) in mice
    Masanobu Sakai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS Noda shi, Chiba, Japan
    J Toxicol Sci 34:483-92. 2009
  10. doi request reprint Regulation of P2X7-dependent inflammatory functions by P2X4 receptor in mouse macrophages
    Ayumi Kawano
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    Biochem Biophys Res Commun 420:102-7. 2012

Collaborators

  • Shuji Kojima
  • Hiroshi Kitani
  • Takato Takenouchi
  • Ken Takeda
  • Mitsuru Sato
  • Takeshi Kondo
  • Eiji Kobayashi
  • Daisuke Mori
  • Hitoshi Harada
  • Yasuhiro Ohshima
  • Erina Takai
  • Hiroko Nakatsukasa
  • Ayumi Kawano
  • Makiko Ishimaru
  • Hayato Sakaki
  • Naoko Nishimaki
  • Yoshinori Moriyama
  • Akihiro Kitami
  • Akihiro Tokunaga
  • Masanobu Sakai
  • Keichiro Yamashita
  • Fumitoshi Tago
  • Chihiro Nagakura
  • Wakako Kaji
  • Shunta Ide
  • Kanako Masumoto
  • Mami Suzuki
  • Shinya Yano
  • Fumie Hattori
  • Taisei Noguchi
  • Emi Satoh
  • Akina Suzuki
  • Emi Sato
  • Nana Tamaishi
  • Akira Awaya
  • Noriko Shimura
  • Nao Shimoda
  • Shigeru Oshio
  • Naoya Takemoto
  • Negishi Yusuke
  • Yusuke Negishi
  • Satomi Tanaka
  • Satomi Itou
  • Yusuke Machino
  • Takuya Fujiwaki
  • Kaori Fukuchi
  • Junya Kohroki
  • Kazunori Akimoto
  • Ayaka Yamanoi
  • Yasuhiko Masuho
  • Shizuka Seki
  • Noriyuki Hotta
  • Keisuke Sawada
  • Yasuko Mutou
  • Yusuke Shinkai
  • Hiroshi Yajima
  • Katsumi Uchida
  • Ayako Masada

Detail Information

Publications37

  1. pmc TGF-β1 Downregulates COX-2 Expression Leading to Decrease of PGE2 Production in Human Lung Cancer A549 Cells, Which Is Involved in Fibrotic Response to TGF-β1
    Erina Takai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda shi, Chiba, Japan
    PLoS ONE 8:e76346. 2013
    ..These results suggest that PGE2 has an anti-fibrotic effect. We conclude that TGF-β1-induced downregulation of COX-2/PGE2 signaling is involved in facilitation of fibrotic EMT response in A549 cells. ..
  2. pmc Involvement of connexin43 hemichannel in ATP release after γ-irradiation
    Yasuhiro Ohshima
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    J Radiat Res 53:551-7. 2012
    ..Our results suggest a novel mechanism for radiation-induced biological effects mediated by both ATP and Cx43...
  3. pmc Autocrine regulation of macrophage activation via exocytosis of ATP and activation of P2Y11 receptor
    Hayato Sakaki
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda shi Chiba, Japan
    PLoS ONE 8:e59778. 2013
    ..Consequently, P2Y11 receptor antagonists may be drug candidates for treatment of inflammatory diseases such as sepsis...
  4. ncbi request reprint Low-dose gamma-ray irradiation induces translocation of Nrf2 into nuclear in mouse macrophage RAW264.7 cells
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, Chiba, Japan
    J Radiat Res 51:349-53. 2010
    ..Our results indicate that low-dose gamma-irradiation induces activation of Nrf2 through ERK1/2-dependent pathways...
  5. doi request reprint Involvement of purinergic signaling in cellular response to gamma radiation
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan
    Radiat Res 173:298-309. 2010
    ..We conclude that ionizing radiation induces release of nucleotides from cells, leading to activation of P2Y receptors, which in turn would result in a variety of biological effects...
  6. doi request reprint Blockade of murine T cell activation by antagonists of P2Y6 and P2X7 receptors
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Biochem Biophys Res Commun 384:512-8. 2009
    ..The P2X7 and P2Y6 receptors were expressed in murine CD4 T cells. In conclusion, our results indicate that activation of P2Y6 and P2X7 receptors contributes to T cell activation via TCR...
  7. ncbi request reprint Repeated 0.5-Gy gamma irradiation attenuates experimental autoimmune encephalomyelitis with up-regulation of regulatory T cells and suppression of IL17 production
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Radiat Res 170:429-36. 2008
    ..These data suggest that low-dose gamma irradiation attenuates EAE through suppression of pro-inflammatory cytokines, reduction of cytotoxic T cells and induction of regulatory T cells...
  8. doi request reprint 0.5 Gy gamma radiation suppresses production of TNF-alpha through up-regulation of MKP-1 in mouse macrophage RAW264.7 cells
    Mitsutoshi Tsukimoto
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Radiat Res 171:219-24. 2009
    ..These results indicate that 0.5 Gy gamma radiation would cause up-regulation of MKP-1, leading to inactivation of p38 MAPK and suppression of TNF-alpha production, in cells of mouse macrophages cell line...
  9. ncbi request reprint Diesel exhaust (DE) aggravates pathology of delayed-type hypersensitivity (DTH) induced by methyl-bovine serum albumin (mBSA) in mice
    Masanobu Sakai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS Noda shi, Chiba, Japan
    J Toxicol Sci 34:483-92. 2009
    ..Our results indicate that DE inhalation may, in part, exacerbate the pathological symptoms of DTH and induction of pro-inflammatory cytokines such as IFN-gamma, TNF-alpha, IL-6 and IL-17...
  10. doi request reprint Regulation of P2X7-dependent inflammatory functions by P2X4 receptor in mouse macrophages
    Ayumi Kawano
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    Biochem Biophys Res Commun 420:102-7. 2012
    ..We conclude that co-expression of P2X4 receptor with P2X7 receptor enhances P2X7-mediated inflammation through both facilitation of release of cytokines and suppression of autophagy...
  11. doi request reprint Autocrine regulation of γ-irradiation-induced DNA damage response via extracellular nucleotides-mediated activation of P2Y6 and P2Y12 receptors
    Naoko Nishimaki
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba 278 0022, Japan
    DNA Repair (Amst) 11:657-65. 2012
    ..We conclude that autocrine/paracrine signaling through P2X7-dependent ATP release and activation of P2Y6 and P2Y12 receptors serves to amplify the cellular response to DNA damage caused by γ-irradiation...
  12. pmc Involvement of SLC17A9-dependent vesicular exocytosis in the mechanism of ATP release during T cell activation
    Akihiro Tokunaga
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi Chiba, Japan
    J Biol Chem 285:17406-16. 2010
    ..In conclusion, vesicular exocytosis of ATP appears to play a role in T cell activation and immune responses...
  13. ncbi request reprint Induction of extracellular ATP mediates increase in intracellular thioredoxin in RAW264.7 cells exposed to low-dose γ-rays
    Yasuhiro Ohshima
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Free Radic Biol Med 51:1240-8. 2011
    ....
  14. doi request reprint Involvement of P2Y6 receptor in p38 MAPK-mediated COX-2 expression in response to UVB irradiation of human keratinocytes
    Erina Takai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi Chiba, Japan
    Radiat Res 175:358-66. 2011
    ..Thus P2Y6 receptor is a mediator of UVB-radiation-induced inflammatory responses in keratinocytes...
  15. doi request reprint Feasibility study of B16 melanoma therapy using oxidized ATP to target purinergic receptor P2X7
    Fumie Hattori
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi Chiba, Japan
    Eur J Pharmacol 695:20-6. 2012
    ..These data indicate that extracellularly released ATP may serve as an intercellular signaling molecule. We propose that the P2X7 receptor is a promising target for treatment of solid tumors...
  16. doi request reprint Involvement of P2X4 receptor in P2X7 receptor-dependent cell death of mouse macrophages
    Ayumi Kawano
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi Chiba 278 8510, Japan
    Biochem Biophys Res Commun 419:374-80. 2012
    ..In conclusion, our results suggest that P2X4 receptor is involved in P2X7 receptor-mediated cell death, but not pore formation or MAPK signaling...
  17. doi request reprint Autocrine regulation of TGF-β1-induced cell migration by exocytosis of ATP and activation of P2 receptors in human lung cancer cells
    Erina Takai
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi Chiba 278 8510, Japan
    J Cell Sci 125:5051-60. 2012
    ..We conclude that autocrine signaling through exocytosis of ATP and activation of P2 receptors is required for the amplification of TGF-β1-induced migration of lung cancer cells...
  18. doi request reprint P2X4 receptor regulates P2X7 receptor-dependent IL-1β and IL-18 release in mouse bone marrow-derived dendritic cells
    Hayato Sakaki
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    Biochem Biophys Res Commun 432:406-11. 2013
    ..We conclude that co-expression of P2X4 receptor with P2X7 receptor in dendritic cells leads to enhancement of inflammation through facilitation of P2X7-dependent release of pro-inflammatory cytokines...
  19. doi request reprint Role of TRPM2 and TRPV1 cation channels in cellular responses to radiation-induced DNA damage
    Kanako Masumoto
    Department of Radiation Biosciences, Tokyo University of Science, Noda shi, Chiba, Japan
    Biochim Biophys Acta 1830:3382-90. 2013
    ..Here, we investigated the contribution of TRPM2 and TRPV1 channels to γ-irradiation- and UVB-induced DNA damage responses in human lung cancer A549 cells...
  20. doi request reprint gamma-Irradiation induces P2X(7) receptor-dependent ATP release from B16 melanoma cells
    Yasuhiro Ohshima
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    Biochim Biophys Acta 1800:40-6. 2010
    ..However, there is no report of radiation-induced ATP release from cancer cells. Here, we examined gamma-irradiation-induced ATP release and its mechanism in B16 melanoma...
  21. doi request reprint Repeated gamma irradiation attenuates collagen-induced arthritis via up-regulation of regulatory T cells but not by damaging lymphocytes directly
    Hiroko Nakatsukasa
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Radiat Res 174:313-24. 2010
    ..These results suggest that a major mechanism of attenuation of the pathology of collagen-induced arthritis by repeated 0.5-Gy gamma irradiation is up-regulation of Treg cells concomitantly with suppression of IL6 and IL17 production...
  22. ncbi request reprint Effect of heterocyclic pyrimidine compounds on UVB-induced cell damage in human keratinocytes and on melanogenesis in mouse B16 cells
    Nao Shimoda
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Biol Pharm Bull 33:862-8. 2010
    ..In conclusion, MS-818 protected epidermal cells from UVB-induced damage and also suppressed melanogenesis in melanoma cells. It appears to be a good candidate for a new UVB-protective and whitening agent for application in cosmetics...
  23. ncbi request reprint Suppressing effect of low-dose gamma-ray irradiation on collagen-induced arthritis
    Hiroko Nakatsukasa
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    J Radiat Res 49:381-9. 2008
    ..Overall, our data suggest that low-dose gamma-ray irradiation could attenuate CIA through suppression of pro-inflammatory cytokines and autoantibody production, and induction of regulatory T cells...
  24. ncbi request reprint Mechanism of pigmentation by minocycline in murine B16 melanoma cells
    Emi Sato
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan
    Yakugaku Zasshi 131:731-8. 2011
    ..The SB203580 inhibited the mRNA expression of tyrosinase and TRP-1, suggesting the minocycline-induced melanogensis occurred via a p38 signaling pathway...
  25. ncbi request reprint P2Y6 receptors and ADAM17 mediate low-dose gamma-ray-induced focus formation (activation) of EGF receptor
    Nana Tamaishi
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba, Japan
    Radiat Res 175:193-200. 2011
    ..We conclude that activation of both P2Y6 receptors and ADAM17 mediates the low-dose γ-radiation-induced activation of EGFR, as evaluated in terms of focus formation, in A549 cells...
  26. ncbi request reprint Purinergic signaling via P2Y receptors up-mediates IL-6 production by liver macrophages/Kupffer cells
    Makiko Ishimaru
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS
    J Toxicol Sci 39:413-23. 2014
    ..Therefore, we first showed the importance of purinergic signaling via P2Y receptors in the activation of Kupffer cells and liver injury, which is worthwhile in drug development for liver diseases. ..
  27. doi request reprint Involvement of P2Y₁₁ receptor in IFN-γ-induced IL-6 production in human keratinocytes
    Makiko Ishimaru
    Department of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Eur J Pharmacol 703:67-73. 2013
    ....
  28. ncbi request reprint Purinergic signaling via P2X7 receptor mediates IL-1β production in Kupffer cells exposed to silica nanoparticle
    Shuji Kojima
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan Electronic address
    Toxicology 321:13-20. 2014
    ..The ROS causes activation of inflammasomes, leading to caspase-1-dependent processing of IL-1β...
  29. doi request reprint Adenosine A2B receptor antagonist suppresses differentiation to regulatory T cells without suppressing activation of T cells
    Hiroko Nakatsukasa
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Yamazaki, Noda shi, Chiba, Japan
    Biochem Biophys Res Commun 409:114-9. 2011
    ..Since A(2B) antagonist blocked differentiation to Treg without suppressing T cell activation, it is possible that blockade of A(2B) receptor would facilitate tumor immunity...
  30. ncbi request reprint Involvement of P2Y11 receptor in silica nanoparticles 30-induced IL-6 production by human keratinocytes
    Chihiro Nagakura
    Department of Radiation Biosciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Toxicology 322:61-8. 2014
    ....
  31. doi request reprint Involvement of P2Y13 receptor in suppression of neuronal differentiation
    Shinya Yano
    Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 3613 Yamazaki, Noda City, Chiba 278 8510, Japan
    Neurosci Lett 518:5-9. 2012
    ..These results suggest involvement of P2Y13 receptor in suppression of neuronal differentiation. Thus, P2Y13 receptor antagonists might be candidates for treatment of neurodegenerative diseases...
  32. ncbi request reprint Repeated 0.5-Gy gamma irradiation attenuates autoimmune disease in MRL-lpr/lpr mice with suppression of CD3+CD4-CD8-B220+ T-cell proliferation and with up-regulation of CD4+CD25+Foxp3+ regulatory T cells
    Fumitoshi Tago
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki Noda shi Chiba, 278 8510, Japan
    Radiat Res 169:59-66. 2008
    ..We therefore conclude that repeated 0.5-Gy gamma irradiation suppresses the proliferation rate of CD3(+)CD4(-)CD8(-)B220(+) T cells and the production of IL6 and autoantibodies and up-regulates regulatory T cells...
  33. doi request reprint Attenuation of delayed-type hypersensitivity by fullerene treatment
    Keichiro Yamashita
    Faculty of Pharmaceutical Sciences, Tokyo University of Science TUS, 2641 Yamazaki, Noda shi, Chiba 278 8510, Japan
    Toxicology 261:19-24. 2009
    ..Nano-C(60) treatment showed significant immunomodulatory effects in a mouse DTH model: IL-6 and IL-17 production was down-regulated, and the Treg cell ratio was up-regulated, concomitantly with attenuation of the pathology of DTH...
  34. ncbi request reprint Purine receptor P2Y6 mediates cellular response to γ-ray-induced DNA damage
    Shunta Ide
    Faculty of Pharmaceutical Sciences, Tokyo University of Science
    J Toxicol Sci 39:15-23. 2014
    ..We found that it was. Taken together, these findings indicate that purinergic signaling through P2Y6 receptor, followed by ERK1/2 activation, promotes the cellular repair response to γ-ray-induced DNA damage. ..
  35. ncbi request reprint Involvement of P2Y receptors in the protective effect of ATP towards the cell damage in HaCaT cells exposed to H₂O₂
    Emi Satoh
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda shi, Chiba, Japan
    J Toxicol Sci 36:741-50. 2011
    ..In conclusion, it was found that ATP protected the cells from the H(2)O(2)-induced cell damages via the P2Y receptor-mediated induction of intracellular antioxidants...
  36. ncbi request reprint Adenosine A(2B) receptor antagonist PSB603 suppresses tumor growth and metastasis by inhibiting induction of regulatory T cells
    Wakako Kaji
    Faculty of Pharmaceutical Sciences, Tokyo University of Science
    J Toxicol Sci 39:191-8. 2014
    ..Overall, our results suggest that A(2B) receptor antagonist PSB603 enhances anti-tumor immunity by inhibiting differentiation to Treg, resulting in a delay of tumor growth and a suppression of metastasis...
  37. doi request reprint Cleavage of the interchain disulfide bonds in rituximab increases its affinity for FcγRIIIA
    Mami Suzuki
    Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Biochem Biophys Res Commun 436:519-24. 2013
    ..These results provide significant information on the structures within IgG1 that are involved in binding FcγRIIIA, and they may be useful in the development of therapeutic antagonists for FcγRIIIA. ..