Kengo Sakaguchi

Summary

Affiliation: Tokyo University of Science
Country: Japan

Publications

  1. ncbi The multi-replication protein A (RPA) system--a new perspective
    Kengo Sakaguchi
    Department of Applied Biological Science, Tokyo University of Science, Chiba, Japan
    FEBS J 276:943-63. 2009
  2. ncbi Applications of zeolite inorganic composites in biotechnology: current state and perspectives
    Kengo Sakaguchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Appl Microbiol Biotechnol 67:306-11. 2005
  3. ncbi Meiosis and small ubiquitin-related modifier (SUMO)-conjugating enzyme, Ubc9
    Kengo Sakaguchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan
    FEBS J 274:3519-31. 2007
  4. ncbi Characterization of the origin recognition complex (ORC) from a higher plant, rice (Oryza sativa L.)
    Yoko Mori
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    Gene 353:23-30. 2005
  5. ncbi Characterization of four RecQ homologues from rice (Oryza sativa L. cv. Nipponbare)
    Ai Saotome
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken, Japan
    Biochem Biophys Res Commun 345:1283-91. 2006
  6. ncbi Functional characterization of two flap endonuclease-1 homologues in rice
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Chiba, 278-8510, Noda, Japan
    Gene 314:63-71. 2003
  7. ncbi Characterization of Rad6 from a higher plant, rice (Oryza sativa L.) and its interaction with Sgt1, a subunit of the SCF ubiquitin ligase complex
    Taichi Yamamoto
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda shi, 278 8510 Chiba ken, Japan
    Biochem Biophys Res Commun 314:434-9. 2004
  8. ncbi Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster
    Tatsushi Ruike
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan
    FEBS J 273:5062-73. 2006
  9. ncbi DmGEN shows a flap endonuclease activity, cleaving the blocked-flap structure and model replication fork
    Yoshihiro Kanai
    Department of Applied Biological Science, Tokyo University of Science, Chiba, Japan
    FEBS J 274:3914-27. 2007
  10. ncbi Site-directed mutational analysis of structural interactions of low molecule compounds binding to the N-terminal 8 kDa domain of DNA polymerase beta
    Shizuka Murakami
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Biochem Biophys Res Commun 350:7-16. 2006

Collaborators

Detail Information

Publications97

  1. ncbi The multi-replication protein A (RPA) system--a new perspective
    Kengo Sakaguchi
    Department of Applied Biological Science, Tokyo University of Science, Chiba, Japan
    FEBS J 276:943-63. 2009
    ..Convergent evolution seems to be ubiquitous in these processes. Using recent findings, we review the composition and biological functions of RPA complexes...
  2. ncbi Applications of zeolite inorganic composites in biotechnology: current state and perspectives
    Kengo Sakaguchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Appl Microbiol Biotechnol 67:306-11. 2005
    ....
  3. ncbi Meiosis and small ubiquitin-related modifier (SUMO)-conjugating enzyme, Ubc9
    Kengo Sakaguchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan
    FEBS J 274:3519-31. 2007
    ..CcUbc9 may also be involved with CcPCNA in the switch from the replicative polymerase being recruited at zygotene to the repair-type DNA polymerases being recruited at pachytene...
  4. ncbi Characterization of the origin recognition complex (ORC) from a higher plant, rice (Oryza sativa L.)
    Yoko Mori
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    Gene 353:23-30. 2005
    ..However, the level of expression of OsORC2 remained constant. We suggest from these results that expression of OsORC1, 3, 4 and 5 are correlated with cell proliferation, but the expression of OsORC2 is not...
  5. ncbi Characterization of four RecQ homologues from rice (Oryza sativa L. cv. Nipponbare)
    Ai Saotome
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken, Japan
    Biochem Biophys Res Commun 345:1283-91. 2006
    ....
  6. ncbi Functional characterization of two flap endonuclease-1 homologues in rice
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Chiba, 278-8510, Noda, Japan
    Gene 314:63-71. 2003
    ..On the other hand, OsFEN-1b cDNA could not complement the rad27 mutant. The roles of OsFEN-1a and OsFEN-1b in plant DNA replication and repair are discussed...
  7. ncbi Characterization of Rad6 from a higher plant, rice (Oryza sativa L.) and its interaction with Sgt1, a subunit of the SCF ubiquitin ligase complex
    Taichi Yamamoto
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda shi, 278 8510 Chiba ken, Japan
    Biochem Biophys Res Commun 314:434-9. 2004
    ..The Rad6 pathway for repair or the proteasome system may thus require Sgt1 as ubiquitin-conjugating enzyme...
  8. ncbi Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster
    Tatsushi Ruike
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan
    FEBS J 273:5062-73. 2006
    ..Our observations suggest that DmPCNA2 may function as an independent sliding clamp of DmPCNA1 when DNA repair occurs...
  9. ncbi DmGEN shows a flap endonuclease activity, cleaving the blocked-flap structure and model replication fork
    Yoshihiro Kanai
    Department of Applied Biological Science, Tokyo University of Science, Chiba, Japan
    FEBS J 274:3914-27. 2007
    ..Thus, our studies revealed that DmGEN belongs to a new class (class IV) of the RAD2/XPG nuclease family. The biochemical properties of DmGEN and its possible role are also discussed...
  10. ncbi Site-directed mutational analysis of structural interactions of low molecule compounds binding to the N-terminal 8 kDa domain of DNA polymerase beta
    Shizuka Murakami
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Biochem Biophys Res Commun 350:7-16. 2006
    ..These findings confirm our previous biochemical and 3D structural proposals concerning inhibition by NA and LCA...
  11. ncbi Higher plant RecA-like protein is homologous to RadA
    Toyotaka Ishibashi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    DNA Repair (Amst) 5:80-8. 2006
    ..An RNAi mutant of Arabidopsis thaliana RadA (AtRadA) was sensitive to mutagenic agents such as UV and MMC, suggesting that RadA functions in DNA repair...
  12. ncbi Interaction between proliferating cell nuclear antigen and JUN-activation-domain-binding protein 1 in the meristem of rice, Oryza sativa L
    Taichi Yamamoto
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda shi, 278 8510, Chiba ken, Japan
    Planta 217:175-83. 2003
    ..The role of OsPCNA with OsJab1 in plant DNA proliferation is discussed...
  13. ncbi Rice UV-damaged DNA binding protein homologues are most abundant in proliferating tissues
    Toyotaka Ishibashi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan
    Gene 308:79-87. 2003
    ..These results indicated that the level of OsUV-DDB expression is correlated with cell proliferation, and its expression may be required mostly for DNA repair in DNA replication...
  14. ncbi Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus
    Taiki Yamaguchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    Fungal Genet Biol 41:493-500. 2004
    ..Northern hybridization analysis indicated CcFEN-1 to be expressed not only in the pre-meiotic S phase but also in meiotic prophase I. The roles of CcFEN-1 during meiosis are discussed...
  15. ncbi Characterization of T-DNA insertion mutants and RNAi silenced plants of Arabidopsis thaliana UV-damaged DNA binding protein 2 (AtUV-DDB2)
    Asami Koga
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Yamazaki, Noda, Chiba, Japan
    Plant Mol Biol 61:227-40. 2006
    ..Our results lead us to suggest that not only does AtUV-DDB2 function in DNA repair, it also has a direct or indirect influence on cell proliferation in the plant meristem...
  16. ncbi Podophyllotoxin directly binds a hinge domain in E2 of HPV and inhibits an E2/E7 interaction in vitro
    Takeki Saitoh
    Genome and Drug Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8501, Japan
    Bioorg Med Chem 16:5815-25. 2008
    ..And we showed the bindings of PT to other types of HPV. Our results suggest that PT is potential as a tool for clarifying the molecular mechanism of HPV...
  17. ncbi DNA ligase IV from a basidiomycete, Coprinus cinereus, and its expression during meiosis
    Satoshi Namekawa
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510 Japan
    Microbiology 149:2119-28. 2003
    ..Intense signals were observed in leptotene and zygotene. Based on these observations, the possible role(s) of C. cinereus DNA ligase IV during meiosis are discussed...
  18. ncbi Drosophila damaged DNA-binding protein 1 is an essential factor for development
    Kei ichi Takata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Genetics 168:855-65. 2004
    ..Correspondingly, all of the D-DDB1 gene knocked-out flies also died. D-DDB1 therefore appears to be an essential development-associated factor in a multicellular organism...
  19. ncbi A screening of a library of T7 phage-displayed peptide identifies E2F-4 as an etoposide-binding protein
    Mihoko Takami
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Molecules 16:4278-94. 2011
    ..Taken together, our results suggest that etoposide directly binds to E2F-4 and inhibits subsequent gene transcription mediated by heterodimeric E2F-4/DP complexes in the nucleus...
  20. ncbi Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ induced by DNA damage
    Taichi Yamamoto
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan
    J Plant Res 118:91-7. 2005
    ..mRNA of DnaJ was induced by UV and DNA-damaging agents such as H2O2. The expression patterns of OsPCNA were almost the same as OsDnaJ. The relationship between OsPCNA and OsDnaJ is discussed...
  21. ncbi Efficient one-cycle affinity selection of binding proteins or peptides specific for a small-molecule using a T7 phage display pool
    Yoichi Takakusagi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 16:9837-46. 2008
    ..Our findings demonstrate the effectiveness of this method and general applicability for a wide range of small-molecules...
  22. ncbi DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis
    Kazuki Iwabata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Nucleic Acids Res 33:5809-18. 2005
    ..The interaction of CcLim15 and CcTopII may facilitate pairing of homologous chromosomes...
  23. ncbi Two types of replication protein A in seed plants
    Toyotaka Ishibashi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan
    FEBS J 272:3270-81. 2005
    ..Each of the two probable plant RPA complexes may have different roles in DNA metabolism...
  24. ncbi Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1: a molecular link between recombination and chromatin assembly during meiosis
    Satomi Ishii
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan
    FEBS J 275:2032-41. 2008
    ..Taken together, these results suggest a novel role of the CAF-1-PCNA complex in meiotic events. We propose that the CAF-1-PCNA complex modulates chromatin assembly following meiotic recombination...
  25. ncbi Proliferating cell nuclear antigen (PCNA) interacts with a meiosis-specific RecA homologues, Lim15/Dmc1, but does not stimulate its strand transfer activity
    Fumika N Hamada
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Biochem Biophys Res Commun 352:836-42. 2007
    ..We propose that the interaction between Lim15/Dmc1 and PCNA mediates the recombination-associated DNA synthesis during meiosis...
  26. ncbi TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster
    Ryoichi Nakamura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi Chiba ken, 278 8510, Japan
    Mol Cell Biol 28:6620-31. 2008
    ..These results suggest that DmTRF4-1 and DmRrp6 are involved in the polyadenylation-mediated degradation of snRNAs in vivo...
  27. ncbi DmGEN, a novel RAD2 family endo-exonuclease from Drosophila melanogaster
    Gen Ishikawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Nucleic Acids Res 32:6251-9. 2004
    ..Homologs are widely found in mammals and higher plants. The data suggest that DmGEN belongs to a new class of RAD2 nuclease...
  28. ncbi Sumoylation of a meiosis-specific RecA homolog, Lim15/Dmc1, via interaction with the small ubiquitin-related modifier (SUMO)-conjugating enzyme Ubc9
    Akiyo Koshiyama
    Department of Applied Biological Science, Tokyo University of Science, Chiba-ken, Japan
    FEBS J 273:4003-12. 2006
    ..These results suggest that sumoylation is a candidate modulator of meiotic recombination via interaction between Ubc9 and Lim15/Dmc1...
  29. ncbi Characterization of DNA polymerase delta from a higher plant, rice (Oryza sativa L.)
    Yukinobu Uchiyama
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Gene 295:19-26. 2002
    ..These results indicated that the levels of OsPoldelta expression are markedly correlated with cell proliferation, and that some of OsPoldelta might have special roles in the leaves...
  30. ncbi Biochemical properties of a plastidial DNA polymerase of rice
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken, 278 8510, Japan
    Plant Mol Biol 64:601-11. 2007
    ..These results provide insights into the possible role of POLPs in plastidial DNA replication and repair...
  31. ncbi Structural relationship of curcumin derivatives binding to the BRCT domain of human DNA polymerase lambda
    Toshifumi Takeuchi
    Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
    Genes Cells 11:223-35. 2006
    ..Based on these results, the pol lambda-inhibitory mechanism of compound 13 is discussed...
  32. ncbi Degradation of proliferating cell nuclear antigen by 26S proteasome in rice (Oryza sativa L.)
    Taichi Yamamoto
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, 278 Chiba-ken, Japan
    Planta 218:640-6. 2004
    ..The tissue-specific expression pattern of the transcripts of Rpt6 and PCNA suggested that the rice proteasome played important roles in DNA replication involving PCNA. These findings indicate a proteasome-dependent degradation of PCNA...
  33. ncbi Plastid DNA polymerases from higher plants, Arabidopsis thaliana
    Yoko Mori
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Biochem Biophys Res Commun 334:43-50. 2005
    ..These results suggested that AtPolI-like B has a role in the repair of oxidation-induced DNA damage. Our data indicate that higher plants possess two plastid DNA polymerases that are not found in animals and yeasts...
  34. ncbi Chemical properties of fatty acid derivatives as inhibitors of DNA polymerases
    Ryoichi Nakamura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Org Biomol Chem 5:3912-21. 2007
    ..Our results suggest that the inhibitory mode of action of PFOdA and PFOS is different from that mediated by the classic fatty acid inhibitors against DNA polymerase beta...
  35. ncbi Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen
    Yukinobu Uchiyama
    Department of Applied Biological Science, Tokyo University of Science, Chiba, 278 8510, Japan
    Planta 227:1233-41. 2008
    ..Bleomycin also increased the fraction of OsXRCC1 associated with chromatin. These results suggest that OsXRCC1 contributes to DNA repair pathways that differ from the mammalian BER system...
  36. ncbi Damaged DNA binding protein 1 in Drosophila defense reactions
    Kei ichi Takata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem Biophys Res Commun 323:1024-31. 2004
    ..Transgenic flies with D-DDB1 gene silencing were found to accumulate abnormal large blood cells, reminiscent of human leukemia, suggesting that D-DDB1 has functions in hemocyte development...
  37. ncbi Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells
    Kaori Shimanouchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278 8510
    J Biochem 139:51-8. 2006
    ..These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells...
  38. ncbi Screening of a library of T7 phage-displayed peptides identifies alphaC helix in 14-3-3 protein as a CBP501-binding site
    Yuki Matsumoto
    Department of Applied Biological Sciences, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 19:7049-56. 2011
    ..Thus, we conclude that in addition to the direct inhibition of Chk kinase activity, CBP501 directly binds to cellular 14-3-3 proteins through alphaC helix...
  39. ncbi Camptothecin (CPT) directly binds to human heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and inhibits the hnRNP A1/topoisomerase I interaction
    Daisuke Manita
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 19:7690-7. 2011
    ..melanogaster...
  40. ncbi Coprinus cinereus DNA ligase I during meiotic development
    Satoshi Namekawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Chiba 278-0022, Noda, Japan
    Biochim Biophys Acta 1627:47-55. 2003
    ..Based on these observations, relationships and roles during the meiotic cell cycle are discussed...
  41. ncbi Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus
    Aiko Sakamoto
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Chromosoma 116:545-56. 2007
    ..Based on these data, we discuss the possible roles of these polymerases in meiosis...
  42. ncbi DNA polymerase mu interacts with a meiosis-specific RecA homolog Lim15 during meiosis in Coprinus cinereus
    Takuro Yanai
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Biochem Biophys Res Commun 390:32-7. 2009
    ..Moreover, CcLim15 enhances polymerase activity of CcPol mu early in the reaction. These results suggest that CcPol mu might be recruited by CcLim15 and elongate the D-loop structure during homologous recombination in meiosis...
  43. ncbi Identification of small molecule binding molecules by affinity purification using a specific ligand immobilized on PEGA resin
    Kouji Kuramochi
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioconjug Chem 19:2417-26. 2008
    ..Our study suggests that PEGA resin has great potential as a tool not only for the purification and identification of small-molecule binding proteins but also for the selection of peptides that recognize target molecules...
  44. ncbi Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
    Yuka Miyano
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 20:3985-90. 2012
    ..Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells...
  45. ncbi Drosophila DNA polymerase zeta interacts with recombination repair protein 1, the Drosophila homologue of human abasic endonuclease 1
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    J Biol Chem 281:11577-85. 2006
    ..On the basis of the direct interaction between DmREV7 and Rrp1, we suggest that Dmpol zeta may be involved in the repair pathway of AP sites in DNA...
  46. ncbi Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography
    Ryo Takeuchi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Biochem J 382:535-43. 2004
    ..The present study is the first report about characterization of purified pol zeta from multicellular organisms, and the second concerning the characterization of yeast pol zeta...
  47. ncbi Sulfoquinovosylmonoacylglycerol inhibitory mode analysis of rat DNA polymerase beta
    Nobuyuki Kasai
    Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
    FEBS J 272:4349-61. 2005
    ..The models show a SQMG interaction interface that is consistent with the data...
  48. ncbi Plant DNA polymerase lambda, a DNA repair enzyme that functions in plant meristematic and meiotic tissues
    Yukinobu Uchiyama
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan
    Eur J Biochem 271:2799-807. 2004
    ..These properties suggest that plant Pol lambda is a DNA repair enzyme which functions in plant meristematic and meiotic tissues, and that it can substitute for Pol beta and terminal deoxyribonucleotidyl transferase...
  49. ncbi [Plastid DNA replication and repair]
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan
    Seikagaku 79:438-41. 2007
  50. ncbi DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, 2641 Yamazaki, Noda-shi, Chiba, 278-8510, Japan
    Nucleic Acids Res 32:2760-7. 2004
    ..These results suggested that photoreactivation is the major DNA repair pathway for the major UV-induced damage in non-proliferating cells, while both photoreactivation and excision repair are active in proliferating cells...
  51. ncbi The expression of the rice (Oryza sativa L.) homologue of Snm1 is induced by DNA damages
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba 278-8510, Japan
    Biochem Biophys Res Commun 329:668-72. 2005
    ..These results suggest that OsSnm1 is involved not only in the repair of DNA interstrand crosslinks, but also in various other DNA repair pathways...
  52. ncbi Identification of antibiotic clarithromycin binding peptide displayed by T7 phage particles
    Tetsuro Morimura
    Genome and Drug Research Center, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan
    J Antibiot (Tokyo) 59:625-32. 2006
    ....
  53. ncbi Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device
    Yoichi Takakusagi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 15:7590-8. 2007
    ..The efficacy of T7 phage display screening for small molecules on QCM devices, target discovery from primary peptide sequence, and application of this strategy to various drug-like small molecules are discussed...
  54. ncbi Coprinus cinereus Mer3 is required for synaptonemal complex formation during meiosis
    Hiroko Sugawara
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan
    Chromosoma 118:127-39. 2009
    ..Our results demonstrate that CcMer3 is required for synaptonemal complex formation after axial elements align and is thus essential for homologous synapsis...
  55. ncbi Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display technology
    Yoichi Takakusagi
    Genome and Drug Discovery Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Bioorg Med Chem Lett 15:4846-9. 2005
    ..After three rounds of selection using C20-biotinylated CPT (CPT-20-B) as bait, a CPT-20-B-binding sequence, NSSQSARR, was identified...
  56. ncbi Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing
    Satoshi H Namekawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan
    Microbiology 151:3669-78. 2005
    ..cinereus research...
  57. ncbi Selective inhibitors of terminal deoxyribonucleotidyltransferase (TdT): baicalin and genistin
    Yukinobu Uchiyama
    Department of Applied Biological Science and Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Biochim Biophys Acta 1725:298-304. 2005
    ..The compounds may, therefore, bind directly to the primer-binding site of TdT and simultaneously disturb dNTP substrate incorporation into the primer. Genistin and baicalin should prove to be useful agents for studying TdT...
  58. ncbi Characterization of marine X-family DNA polymerases and comparative analysis of base excision repair proteins
    Hirofumi Kodera
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Biochem Biophys Res Commun 415:193-9. 2011
    ..On the basis of our findings and previous reports, we discuss possible evolutional model of Pol β and the other members of the Pol X family...
  59. ncbi Effective form of sulfoquinovosyldiacyglycerol (SQDG) vesicles for DNA polymerase inhibition
    Kenjiro Matsumoto
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278, Japan
    Colloids Surf B Biointerfaces 46:175-81. 2005
    ..beta-SQDG-C(18:0) vesicles, especially those of small size, were effective in DNA polymerase inhibition and are expected to have high applicability in DNA polymerase study...
  60. ncbi An emulsion of sulfoquinovosylacylglycerol with long-chain alkanes increases its permeability to tumor cells
    Satoko Aoki
    Department of Applied of Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    J Membr Biol 213:11-8. 2006
    ..Based on these data, we discuss the relationship between the molecular action of and antitumor activity by alpha-SQMG-C(18:0)...
  61. ncbi Molecular design of cholesterols as inhibitors of DNA polymerase alpha
    Masahiko Oshige
    Frontier Research Center for Genome and Drug Discovery, Department of Applied Biological Science, Faculty of Science and Technology, and Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    J Med Chem 47:4971-4. 2004
    ..alpha activity. With pol.alpha, these compounds acted by competing with the template-primer DNA and noncompetitively with the substrate...
  62. ncbi [Latest frontiers of meiosis research in Coprinus cinereus]
    Satoshi H Namekawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    Seikagaku 76:1450-4. 2004
  63. ncbi Mammalian mitotic centromere-associated kinesin (MCAK): a new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents
    Satoko Aoki
    Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
    FEBS J 272:2132-40. 2005
    ..It should be stressed that inhibitors of MCAK have never been reported previously so that there is a major potential for clinical utility...
  64. ncbi Heterogeneous nucleation of protein crystals on fluorinated layered silicate
    Keita Ino
    Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
    PLoS ONE 6:e22582. 2011
    ..Adsorption experiments of proteins on the saponites confirmed that the density of adsorbed molecules increased according to the substitution rate, thereby explaining the heterogeneous nucleation on the silicate surface...
  65. ncbi Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B
    Kengo Morohashi
    Genome and Drug Research Center, Tokyo University of Science, Noda, Chiba, Japan
    PLoS ONE 6:e18285. 2011
    ..However, the regulation mechanisms of HCV replication governed by CsA target proteins have not been fully understood...
  66. ncbi DNA repair in plants
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba, Japan
    Chem Rev 106:753-66. 2006
  67. ncbi Syntheses and applications of fluorescent and biotinylated epolactaene derivatives: Epolactaene and its derivative induce disulfide formation
    Kouji Kuramochi
    Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Bioorg Med Chem 16:5039-49. 2008
    ..The results obtained from the reaction of epolactaene or its derivative with N-acetylcysteine methyl ester indicate that these compounds induce the disulfide formation and the alpha-position of the epoxylactam core is the reactive site...
  68. ncbi Selective adsorption of bacterial cells onto zeolites
    Munehiro Kubota
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Colloids Surf B Biointerfaces 64:88-97. 2008
    ..Zeolites could adsorb each of the bacterial cell species with high selectivity even from a mixed suspension. Zeolites can therefore be used as effective carrier materials to provide an easy, rapid and accurate method for cell separation...
  69. ncbi Affinity capture of a mammalian DNA polymerase beta by inhibitors immobilized to resins used in solid-phase organic synthesis
    Kouji Kuramochi
    Genome and Drug Research Center, Tokyo University of Science, Noda, Chiba 278-8510, Japan
    Bioconjug Chem 16:97-104. 2005
    ..The pol beta inhibitors LCA, C18-beta-SQDG, and epolactaene were immobilized on the photoaffinity beads by photoreaction. The batch affinity purification of pol beta from a protein mixture could be also achieved with these beads...
  70. ncbi Zeolites as new chromatographic carriers for proteins--easy recovery of proteins adsorbed on zeolites by polyethylene glycol
    Hiroyuki Chiku
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda-shi, Chiba-ken 278-8510, Japan
    Anal Biochem 318:80-5. 2003
    ..Using this method for the adsorption and desorption of proteins at pI, we succeeded in separating various proteins. The application of zeolites to biochemistry and biotechnology is also discussed...
  71. ncbi Use of layer silicate for protein crystallization: effects of Micromica and chlorite powders in hanging drops
    Masahide Takehara
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278 8510, Japan
    Anal Biochem 373:322-9. 2008
    ..The effect of Micromica and chlorite powders and the application of layer silicate powder for protein crystallization are discussed...
  72. ncbi Dissociation of DNA polymerase alpha-primase complex during meiosis in Coprinus cinereus
    Satoshi Namekawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan
    Eur J Biochem 270:2137-46. 2003
    ..These results suggest that the form of the DNA polymerase alpha-primase complex is altered during meiotic development...
  73. ncbi A method for the molecular imprinting of hemoglobin on silica surfaces using silanes
    Toru Shiomi
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
    Biomaterials 26:5564-71. 2005
    ..The results suggested the capacity for selective adsorption of MIPi to be not only based on the isoelectric point (pI) and protein molecular weight, but also the characteristics of protein recognition cavities imprinted on base silica...
  74. ncbi A novel DNA polymerase homologous to Escherichia coli DNA polymerase I from a higher plant, rice (Oryza sativa L.)
    Seisuke Kimura
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Nucleic Acids Res 30:1585-92. 2002
    ..These properties indicated that OsPolI-like is a novel plant DNA polymerase. The function of OsPolI-like is discussed in relation to plastid maturation...
  75. ncbi OsSEND-1: a new RAD2 nuclease family member in higher plants
    Tomoyuki Furukawa
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
    Plant Mol Biol 51:59-70. 2003
    ..The mRNA level of OsSEND-1 was induced by UV and DNA-damaging agent such as MMS or H2O2, indicating that OsSEND-1 has some roles in the repair of many types of damaged DNA. The recombinant peptide showed endonuclease activity...
  76. ncbi Cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica
    Munehiro Kubota
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamzaki, Noda-shi, Chiba-ken 278-8510, Japan
    Appl Environ Microbiol 71:8895-902. 2005
    ..Most of the desorbed cells were alive. Thus, quantitative and selective fractionation of cells is readily achievable by employing chitosan, a known antibacterial material...
  77. ncbi A novel protein refolding method using a zeolite
    Hiroyuki Chiku
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan
    Anal Biochem 348:307-14. 2006
    ..The refolded proteins are biochemically active, and NMR analysis of one of the proteins (replication protein A 8) supports the conclusion that correct refolding does occur. Based on these results, we discuss the refolding mechanism...
  78. ncbi Distribution and roles of X-family DNA polymerases in eukaryotes
    Yukinobu Uchiyama
    Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
    Biochimie 91:165-70. 2009
    ..In this review, we focus on the interesting pattern of distribution of the X-family enzymes across biological kingdoms and speculate on their roles...
  79. ncbi Synthesis of an immunosuppressant SQAG9 and determination of the binding peptide by T7 phage display
    Takayuki Yamazaki
    Genome and Drug Research Center, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan
    Bioorg Med Chem Lett 14:4343-6. 2004
    ..The immobilized synthetic peptide on a sensor chip showed a dissociation constant of K(D)=1.5 x 10(-6) against SQAG9 in a surface plasmon resonance experiment...
  80. ncbi Leucine aminopeptidase during meiotic development
    Takashi Ishizaki
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan
    Eur J Biochem 269:826-32. 2002
    ..Moreover, the strong expression of CoLAP mRNA immediately after treatment with methyl methanesulfonate in mycelium implies that CoLAP has a role in somatic DNA repair...
  81. ncbi Drosophila damage-specific DNA-binding protein 1 (D-DDB1) is controlled by the DRE/DREF system
    Kei ichi Takata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Nucleic Acids Res 30:3795-808. 2002
    ..The results indicate that D-DDB1 not only contributes to the DNA repair system, but also has a role in cell proliferation and development...
  82. ncbi [DNA repair in higher plants]
    Seisuke Kimura
    Department of Applied Biological Sciences, Faculty of Sciences and Technology, Tokyo University of Science, Noda-shi, Chiba, Japan
    Seikagaku 77:113-23. 2005
  83. ncbi Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development
    Kei ichi Takata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda shi, Chiba ken 278 8510, Japan
    Cell Biol Int 27:361-74. 2003
    ..The results suggest that D-mtTFA not only contributes to the mitochondrial DNA transcription and replication system, but also has a role in cell proliferation and development, including spermatogenesis...
  84. ncbi Inhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi ku, Hyogo 651 2180, Japan
    Int J Mol Med 21:281-90. 2008
    ..These results suggested that, at least in vitro, oleic acid-containing cell membranes of the lipid bilayer inhibit Cdt1-geminin complex formation by binding to Cdt1 and thereby liberating Cdt1 from inhibition by geminin...
  85. ncbi The ensemble of hetero-proteins in inorganic nanochannels
    Shun ichi Matsuura
    Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology, Nigatake 4 2 1, Miyagino ku, Sendai 983 8551, Japan
    Bioconjug Chem 19:10-4. 2008
    ..Furthermore, an increase in the amount of sGFP and DsRed adsorbed to the pores of FSM6.2 led to a decrease in the distance between these proteins, resulting in enhancement of FRET efficiency...
  86. ncbi Structure-activity relationship of a glycolipid, sulfoquinovosyl diacylglycerol, with the DNA binding activity of p53
    Hiroshi Iijima
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Hyogo 651 2180, Japan
    Int J Mol Med 19:41-8. 2007
    ....
  87. ncbi Structural relationship of lithocholic acid derivatives binding to the N-terminal 8-kDa domain of DNA polymerase beta
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, and High Technology Research Center, Kobe Gakuin University, Nishi ku, Kobe, Hyogo 651 2180, Japan
    Biochemistry 43:10669-77. 2004
    ..From these results, the structure-function relationship among pol beta and its selective inhibitors was discussed...
  88. ncbi Spatial distribution of the 26S proteasome in meristematic tissues and primordia of rice (Oryza sativa L.)
    Yuki Yanagawa
    Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Japan
    Planta 214:703-7. 2002
    ..Our results suggest that the 26S proteasome is spatially distributed among various tissues and may be involved not only in cell division but also in organ formation in higher plants...
  89. ncbi The inhibitory action of long-chain fatty acids on the DNA binding activity of p53
    Hiroshi Iijima
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University, Nishi-ku, Kobe, Hyogo 651-2180, Japan
    Lipids 41:521-7. 2006
    ....
  90. ncbi Proliferating cell nuclear antigen from a basidiomycete, Coprinus cinereus. Alternative truncation and expression in meiosis
    Fumika Hamada
    Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Japan
    Eur J Biochem 269:164-74. 2002
    ..Western blotting analysis using anti-(CoPCNA-alpha) Ig revealed that at least two CoPCNA mRNAs before and after truncation were translated at the meiotic prophase as CoPCNA-alpha and CoPCNA-beta...
  91. ncbi Plant-specific regulation of replication protein A2 (OsRPA2) from rice during the cell cycle and in response to ultraviolet light exposure
    Tanja Marwedel
    , , Ohnhorststrasse 18, 22609 Hamburg, Germany
    Planta 217:457-65. 2003
    ....
  92. ncbi The inhibitory action of pyrrolidine alkaloid, 1,4-dideoxy-1,4-imino-D-ribitol, on eukaryotic DNA polymerases
    Yoshiyuki Mizushina
    Department of Nutritional Science, Laboratory of Food and Nutritional Sciences, Kobe Gakuin University, Nishi ku, Kobe, 651 2180, Hyogo, Japan
    Biochem Biophys Res Commun 304:78-85. 2003
    ..The relationship between the structure of DRB and the inhibition of eukaryotic DNA polymerases is discussed...
  93. ncbi Coenzyme Q10 as a potent compound that inhibits Cdt1-geminin interaction
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe Gakuin University, Nishi ku, Kobe, Hyogo 651 2180, Japan
    Biochim Biophys Acta 1780:203-13. 2008
    ..The application of inhibitors for the formation of Cdt1-geminin complex is discussed...
  94. ncbi Flavonoid glycoside: a new inhibitor of eukaryotic DNA polymerase alpha and a new carrier for inhibitor-affinity chromatography
    Yoshiyuki Mizushina
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science and High Tecnology Research Center, Kobe Gakuin University, Nishi ku, Kobe, Hyogo 651 2180, Japan
    Biochem Biophys Res Commun 301:480-7. 2003
    ..We then made a KAG-conjugated column such as the epoxy-activated Sepharose 6B. In the column, pol alpha was selectively adsorbed and eluted...
  95. ncbi Characterization of all the subunits of replication factor C from a higher plant, rice (Oryza sativa L.), and their relation to development
    Tomoyuki Furukawa
    National Institute of Agrobiological Sciences, 2-1-2 Tsukuba-shi, Ibaraki-ken 305-8602, Japan
    Plant Mol Biol 53:15-25. 2003
    ..These results suggest the functional differences of each OsRFC subunit in tissues and the plant cell cycle. The roles of these molecules in plant DNA replication and DNA repair are discussed...
  96. ncbi Inhibition of CD62L+ T-cell response in vitro via a novel sulfo-glycolipid, beta-SQAG9 liposome that binds to CD62L molecule on the cell surface
    Yoshiteru Yamamoto
    Division of Plastic Surgery, Sapporo Medical University School of Medicine, South 1 West 17, Chuo-ku, Sapporo 060-8556, Japan
    Cell Immunol 232:105-15. 2004
    ..These findings suggested beta-SQAG9 to be a novel drug with a unique immunosuppressive action...
  97. ncbi Vitamin A-related compounds, all-trans retinal and retinoic acids, selectively inhibit activities of mammalian replicative DNA polymerases
    Chikako Murakami
    Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University, Hyogo, Japan
    Biochim Biophys Acta 1574:85-92. 2002
    ..The cells were halted at G1 phase in the cell cycle by RAL...