Motoyoshi Nomizu

Summary

Affiliation: Tokyo University of Pharmacy and Life Science
Country: Japan

Publications

  1. ncbi request reprint [Laminin active peptides as a powerful tool for biomedical materials]
    Mayumi Mochizuki
    Tanpakushitsu Kakusan Koso 50:374-82. 2005
  2. ncbi request reprint Preparation and characterization of laminin-derived peptide AG73-coated liposomes as a selective gene delivery tool
    Yoichi Negishi
    Department of Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Japan
    Biol Pharm Bull 33:1766-9. 2010
  3. ncbi request reprint The LG1-3 tandem of laminin alpha5 harbors the binding sites of Lutheran/basal cell adhesion molecule and alpha3beta1/alpha6beta1 integrins
    Yamato Kikkawa
    Renal Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:14853-60. 2007
  4. doi request reprint The influence of synthetic peptides derived from the laminin alpha1 chain on hepatocyte adhesion and gene expression
    Yamato Kikkawa
    Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Hachioji, Tokyo 192 0392, Japan
    Biomaterials 30:6888-95. 2009
  5. doi request reprint Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound
    Yoichi Negishi
    Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Pharm 7:217-26. 2010
  6. doi request reprint Identification of an active site of EMMPRIN for the augmentation of matrix metalloproteinase-1 and -3 expression in a co-culture of human uterine cervical carcinoma cells and fibroblasts
    Takashi Sato
    Department of Biochemistry and Molecular Biology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Gynecol Oncol 114:337-42. 2009
  7. ncbi request reprint The influence of Tribenoside on expression and deposition of epidermal laminins in HaCaT cells
    Yamato Kikkawa
    Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Biol Pharm Bull 33:307-10. 2010
  8. ncbi request reprint Identification of an active site on the laminin alpha5 chain globular domain that binds to CD44 and inhibits malignancy
    Suguru Hibino
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research NIH, 30 Convent Drive, Bethesda, MD 20892, USA
    Cancer Res 64:4810-6. 2004
  9. ncbi request reprint Localization of the laminin alpha4 chain in the skin and identification of a heparin-dependent cell adhesion site within the laminin alpha4 chain C-terminal LG4 module
    Hiroshi Matsuura
    Department of Dermatology, Graduate School of Medicine, Chiba University, Chiba, Japan
    J Invest Dermatol 122:614-20. 2004
  10. ncbi request reprint Cyclic peptides from the loop region of the laminin alpha 4 chain LG4 module show enhanced biological activity over linear peptides
    Fumiharu Yokoyama
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochemistry 43:13590-7. 2004

Collaborators

Detail Information

Publications44

  1. ncbi request reprint [Laminin active peptides as a powerful tool for biomedical materials]
    Mayumi Mochizuki
    Tanpakushitsu Kakusan Koso 50:374-82. 2005
  2. ncbi request reprint Preparation and characterization of laminin-derived peptide AG73-coated liposomes as a selective gene delivery tool
    Yoichi Negishi
    Department of Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Japan
    Biol Pharm Bull 33:1766-9. 2010
    ..Furthermore, our results suggest that AG73-PEG liposomes can be a useful targeted gene delivery vehicle for syndecan-2 overexpressing cancer cells...
  3. ncbi request reprint The LG1-3 tandem of laminin alpha5 harbors the binding sites of Lutheran/basal cell adhesion molecule and alpha3beta1/alpha6beta1 integrins
    Yamato Kikkawa
    Renal Division, Department of Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:14853-60. 2007
    ..Our results also suggest that the binding site for Lu/B-CAM on laminin alpha5 may overlap with that of integrins alpha3beta1 and alpha6beta1...
  4. doi request reprint The influence of synthetic peptides derived from the laminin alpha1 chain on hepatocyte adhesion and gene expression
    Yamato Kikkawa
    Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Hachioji, Tokyo 192 0392, Japan
    Biomaterials 30:6888-95. 2009
    ..The amino acid sequence of A13 peptide was required for regulating hepatocyte behavior. The hepatocyte adhesive peptides can be utilized in tailoring synthetic biomaterials in order to achieve a specific cellular response...
  5. doi request reprint Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound
    Yoichi Negishi
    Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Pharm 7:217-26. 2010
    ..Thus, the combination of AG73-PEG liposomes with Bubble liposomes and ultrasound exposure may be a promising method to achieve selective and efficient gene delivery for cancer therapy...
  6. doi request reprint Identification of an active site of EMMPRIN for the augmentation of matrix metalloproteinase-1 and -3 expression in a co-culture of human uterine cervical carcinoma cells and fibroblasts
    Takashi Sato
    Department of Biochemistry and Molecular Biology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Gynecol Oncol 114:337-42. 2009
    ..Gynecol Oncol 2004; 92:47-56). The aim of this study was to clarify the active site of EMMPRIN in the augmentation of MMP production in the co-culture of SKG-II cells and HUCF...
  7. ncbi request reprint The influence of Tribenoside on expression and deposition of epidermal laminins in HaCaT cells
    Yamato Kikkawa
    Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Biol Pharm Bull 33:307-10. 2010
    ..These results suggest that tribenoside interacts with epidermal cells and regulates the expression and localization of laminins to help reconstruct BMs in wound healing of hemorrhoids...
  8. ncbi request reprint Identification of an active site on the laminin alpha5 chain globular domain that binds to CD44 and inhibits malignancy
    Suguru Hibino
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research NIH, 30 Convent Drive, Bethesda, MD 20892, USA
    Cancer Res 64:4810-6. 2004
    ..Our results suggest that A5G27 binds to the CD44 receptor of B16-F10 melanoma cells via the GAGs on CD44 and, thus, inhibits tumor cell migration, invasion, and angiogenesis in a dominant-negative manner...
  9. ncbi request reprint Localization of the laminin alpha4 chain in the skin and identification of a heparin-dependent cell adhesion site within the laminin alpha4 chain C-terminal LG4 module
    Hiroshi Matsuura
    Department of Dermatology, Graduate School of Medicine, Chiba University, Chiba, Japan
    J Invest Dermatol 122:614-20. 2004
    ..Our study suggests that the laminin alpha4 LG4 module may play an important role in cell adhesion and/or vessel wall formation in the skin by interacting with syndecan-2 and/or -4...
  10. ncbi request reprint Cyclic peptides from the loop region of the laminin alpha 4 chain LG4 module show enhanced biological activity over linear peptides
    Fumiharu Yokoyama
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochemistry 43:13590-7. 2004
    ..These results indicate that the activity of A4G82 is highly conformation-dependent, suggesting that the E-F loop structure is crucial for its biological activity...
  11. ncbi request reprint Cyclic peptide analysis of the biologically active loop region in the laminin alpha3 chain LG4 module demonstrates the importance of peptide conformation on biological activity
    Kozue Kato-Takagaki
    School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Biochemistry 46:1952-60. 2007
    ..The cyclo-hEF3A peptide may be useful for the development of therapeutic reagents especially for wound healing and nerve regeneration...
  12. ncbi request reprint Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling
    Atsushi Utani
    Department of Dermatology, Graduate School of Medicine, Chiba University, Chiba 260 8670, Japan
    J Biol Chem 278:34483-90. 2003
    ..Our study suggests that the laminin alpha 3 LG4 module may play an important role in tissue remodeling by inducing MMP-1 expression during wound healing...
  13. ncbi request reprint Identification of neurite outgrowth active sites on the laminin alpha4 chain G domain
    Naoki Ichikawa
    Research Institute for Diseases of Old Age, Department of Neurology, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    Biochemistry 44:5755-62. 2005
    ..Taken together, the data suggest that the peptides from the laminin alpha4 chain G domain promote neurite outgrowth activity via a specific conformation...
  14. ncbi request reprint Laminin alpha1 chain LG4 module promotes cell attachment through syndecans and cell spreading through integrin alpha2beta1
    Kentaro Hozumi
    Molecular Biology Section, Craniofacial Developmental Biology and Regeneration Branch, NIDCR, National Institutes of Health, Bethesda, MD 20892 4370, USA
    J Biol Chem 281:32929-40. 2006
    ..Our findings indicate that LG4 has a unique function distinct from laminin-1 and suggest that laminin alpha1 LG4-5 may also be produced by a proteolytic cleavage in certain tissues where it exerts its activity...
  15. ncbi request reprint Functional sites in the laminin alpha chains
    Nobuharu Suzuki
    School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Connect Tissue Res 46:142-52. 2005
    ..Laminins possess multifunctional sequences and are key molecules that determine cell fate...
  16. ncbi request reprint Bifunctional peptides derived from homologous loop regions in the laminin alpha chain LG4 modules interact with both alpha 2 beta 1 integrin and syndecan-2
    Fumiharu Yokoyama
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochemistry 44:9581-9. 2005
    ..We conclude that the approach utilizing chimeric peptides is useful for examining cellular mechanisms in dual-receptor systems...
  17. ncbi request reprint Laminin peptide-conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin
    Shigehiro Ikemoto
    Department of Plastic and Aesthetic Surgery, Kitasato University School of Medicine, Sagamihara, Japan
    J Biomed Mater Res A 79:716-22. 2006
    ..We suggest that the AG73-chitosan membrane is useful as a therapeutic formulation and is applicable as a cell delivery system such as delivering keratinocytes to a wound bed...
  18. ncbi request reprint Integrin-dependent cell behavior on ECM peptide-conjugated chitosan membranes
    Mayumi Mochizuki
    Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
    Biopolymers 88:122-30. 2007
    ..These data demonstrate that peptide-chitosan membranes can regulate specific integrin-mediated cell responses and are useful constructs as ECM mimetics...
  19. ncbi request reprint Identification of cell binding sites in the laminin alpha5-chain G domain
    Masayoshi Makino
    Graduate School of Environmental Earth Science, Hokkaido University, Kita 10 Nishi 5, Kita ku, Sapporo 060 0810, Japan
    Exp Cell Res 277:95-106. 2002
    ..Therefore, these peptides may be useful in the investigation of laminin-receptor interactions and possibly mechanisms of laminin signal transduction...
  20. ncbi request reprint Identification of neurite outgrowth promoting sites on the laminin alpha 3 chain G domain
    Kozue Kato
    Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochemistry 41:10747-53. 2002
    ..These results suggest that the A3G75- and A3G83-conjugated chitosan membranes are applicable as bio-medical materials for neural tissue repair and engineering...
  21. doi request reprint Laminin alpha 5 mediates ectopic adhesion of hepatocellular carcinoma through integrins and/or Lutheran/basal cell adhesion molecule
    Yamato Kikkawa
    Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji 192 0392, Japan
    Exp Cell Res 314:2579-90. 2008
    ..These results suggest that laminins containing alpha 5 serve as functional substrates regulating progression of HCC...
  22. ncbi request reprint Laminin-1 peptide-conjugated chitosan membranes as a novel approach for cell engineering
    Mayumi Mochizuki
    Laboratory of Bio Material Chemistry, Division of Bioscience, Graduate School of Environmental Earth Science Hokkaido University, Kita 10 Nishi 5, Kita ku, Sapporo 060 0810, Japan
    FASEB J 17:875-7. 2003
    ....
  23. ncbi request reprint Biological activities of homologous loop regions in the laminin alpha chain G domains
    Nobuharu Suzuki
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    J Biol Chem 278:45697-705. 2003
    ..The homologous peptides, which promote either integrin- or syndecan-mediated cell attachment, may be useful for understanding the cell type- and chain-specific biological activities of the laminins...
  24. ncbi request reprint Identification of a potent peptide antagonist to an active laminin-1 sequence that blocks angiogenesis and tumor growth
    M Lourdes Ponce
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Res 63:5060-4. 2003
    ..These results indicate that we have identified a more potent antiangiogenesis inhibitor peptide that may be used as a therapeutic to treat cancer...
  25. ncbi request reprint Role for laminin-alpha5 chain LG4 module in epithelial branching morphogenesis
    Yuichi Kadoya
    Department of Anatomy, Kitasato University School of Medicine, Sagamihara 228 8555, Japan
    Dev Biol 263:153-64. 2003
    ..A5G77f also perturbed in vitro development of lung and kidney. These results suggest a crucial role for the LG4 module of laminin-alpha5 in epithelial morphogenesis that is distinct from that of the laminin-alpha1 LG4...
  26. ncbi request reprint Syndecan binding sites in the laminin alpha1 chain G domain
    Nobuharu Suzuki
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochemistry 42:12625-33. 2003
    ..These sites may play a critical role in the diverse biological activities involving heparin and syndecan-4 binding...
  27. ncbi request reprint Preparation and biological activities of a bivalent poly(ethylene glycol) hybrid containing an active site and its synergistic site of fibronectin
    Yuichi Susuki
    Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University, Japan
    Chem Pharm Bull (Tokyo) 50:1229-32. 2002
    ..PEG may serve as a spacer for maintaining the desired spatial array...
  28. ncbi request reprint Angiogenic activity of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT)
    Mayumi Mochizuki
    Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Arch Biochem Biophys 459:249-55. 2007
    ..These results indicate that the AG73 peptide is a potent syndecan-binding angiogenesis stimulator and may be useful for therapeutic application to treat ischemic injuries...
  29. ncbi request reprint Interaction of arginine-rich peptides with membrane-associated proteoglycans is crucial for induction of actin organization and macropinocytosis
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 46:492-501. 2007
    ..These data strongly suggest the possibility that the interaction of the arginine-rich peptides with the membrane-associated proteoglycans quickly activates the intracellular signals and induces actin organization and macropinocytotis...
  30. ncbi request reprint Identification of multiple amyloidogenic sequences in laminin-1
    Shingo Kasai
    Tokyo University of Pharmacy and Life Science, Tokyo 192 0392, Japan
    Biochemistry 46:3966-74. 2007
    ..We conclude that the laminin-derived amyloidogenic peptides have the potential to form amyloid-like fibrils in vivo, possibly when laminin-1 is degraded...
  31. ncbi request reprint Laminin alpha5 chain metastasis- and angiogenesis-inhibiting peptide blocks fibroblast growth factor 2 activity by binding to the heparan sulfate chains of CD44
    Suguru Hibino
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland 20892 4370, USA
    Cancer Res 65:10494-501. 2005
    ..We conclude that peptide A5G27 inhibits metastasis and angiogenesis by blocking FGF2 binding to the heparan sulfate side chains of CD44 variant 3, thus decreasing FGF2 bioactivity...
  32. ncbi request reprint Identification of biologically active sequences in the laminin alpha 4 chain G domain
    Ikuko Okazaki
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    J Biol Chem 277:37070-8. 2002
    ..These active peptides may be useful for defining of the molecular mechanism laminin-receptor interactions and laminin-mediated cellular signaling pathways...
  33. ncbi request reprint Ile-Lys-Val-Ala-Val (IKVAV)-containing laminin alpha1 chain peptides form amyloid-like fibrils
    Masanori Yamada
    Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita 10 Nishi 5, Kita ku, Sapporo 060 0810, Japan
    FEBS Lett 530:48-52. 2002
    ..We also examined amyloid-like fibril formation of LAM-L analogs. The neurite outgrowth activity of the LAM-L analogs was closely related to their amyloid-like fibril formation...
  34. ncbi request reprint An association of IgG anti-laminin-1 autoantibodies with endometriosis in infertile patients
    Junko Inagaki
    Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Hum Reprod 18:544-9. 2003
    ..The present study assessed the clinical significance of anti-laminin-1 Abs with infertility, accompanied with or without endometriosis...
  35. ncbi request reprint Blood compatible aspects of DNA-modified polysulfone membrane-protein adsorption and platelet adhesion
    Changsheng Zhao
    Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, 060 0810, Sapporo, Japan
    Biomaterials 24:3747-55. 2003
    ..This suggested that DNA can be regarded as a biopolymer to modify PSf, and contributes to the hydrophilic and hemocompatible wipers on the surface of the hydrophobic PSf membranes...
  36. ncbi request reprint UV-irradiated DNA matrixes selectively bind endocrine disruptors with a planar structure
    Masanori Yamada
    Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan
    Environ Sci Technol 36:949-54. 2002
    ..DNA-immobilized glass beads and DNA-films prepared by UV irradiation have the potential to serve as useful biomaterials for medical, engineering, and environmental applications...
  37. ncbi request reprint Immunization of naïve mice with mouse laminin-1 affected pregnancy outcome in a mouse model
    Shelly Tartakover Matalon
    Research Center for Autoimmune Diseases, Department of Medicine B Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
    Am J Reprod Immunol 50:159-65. 2003
    ..Laminins have important roles during placental and embryonic development. The aim of our study was to determine if active immunization of mice with laminin-1 could elicit an autoimmune response, and induce features of reproductive failure...
  38. ncbi request reprint Multifunctional peptide fibrils for biomedical materials
    Shingo Kasai
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biopolymers 76:27-33. 2004
    ..We conclude that multifunctional peptide fibrils can be designed by conjugation of active peptides on A208 and that this construct has potential to serve as a bioadhesive for tissue regeneration and engineering...
  39. ncbi request reprint Active sites in the carboxyl-terminal region of the laminin alpha chain in Drosophila neuronal cell spreading
    Yasumitsu Takagi
    Hirohashi Cell Configuration Project, Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Corporation JSTC, Tsukuba Research Consortium, Tsukuba, Japan
    Arch Insect Biochem Physiol 56:162-9. 2004
    ..Furthermore, laminin-dependent BG2-c6 cell spreading was inhibited by heparin. This observation suggests that cell surface glycoproteins participate in the interaction of laminin and BG2-c6 cells...
  40. pmc Characterization of a murine anti-laminin-1 monoclonal antibody (AK8) produced by immunization with mouse-derived laminin-1
    Akane Kondo
    Department of Obstetrics and Gynecology, Center for Specialized Clinical Science, Tokai University, School of Medicine, Ishehara, Japan
    Clin Dev Immunol 12:67-73. 2005
    ..The passive immunization of mice with AK8 mAb may represent a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure...
  41. ncbi request reprint Effect of DNA structure on the formation of collagen-DNA complex
    Michiko Kaya
    Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Int J Biol Macromol 35:39-46. 2005
    ..DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms...
  42. ncbi request reprint Laminin alpha3 LG4 module induces keratinocyte migration: involvement of matrix metalloproteinase-9
    Yutaka Momota
    Department of Dermatology, Graduate School of Medicine, Chiba University, Chiba, Japan
    J Recept Signal Transduct Res 25:1-17. 2005
    ..Taken these together, laminin-5 alpha3 LG4 module may play an important role in re-epithelialization at tissue remodeling...
  43. ncbi request reprint Characterization of a fasciclin I-like protein with cell attachment activity from sea urchin (Strongylocentrotus intermedius) ovaries
    Koji Sato
    Division of Bio Science, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Arch Biochem Biophys 424:1-10. 2004
    ..The protein has a potential to be involved in biological functions in sea urchin as a cell adhesive molecule...
  44. ncbi request reprint Inhibition of hair follicle growth by a laminin-1 G-domain peptide, RKRLQVQLSIRT, in an organ culture of isolated vibrissa rudiment
    Kazuhiro Hayashi
    Department of Plastic and Reconstructive Surgery, Kitasato University School of Medicine, Kitasato, Sagamihara, Japan
    J Invest Dermatol 118:712-8. 2002
    ..We also found a region-specific loss of the laminin-alpha1 chain in the basement membrane at the distal region of the invading hair follicle epithelium, indicating that laminins play a part in hair morphogenesis...