Shigeo Murata

Summary

Affiliation: Tokyo Metropolitan Institute of Medical Science
Country: Japan

Publications

  1. ncbi Regulation of CD8+ T cell development by thymus-specific proteasomes
    Shigeo Murata
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Science 316:1349-53. 2007
  2. ncbi Multiple chaperone-assisted formation of mammalian 20S proteasomes
    Shigeo Murata
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    IUBMB Life 58:344-8. 2006
  3. ncbi [Immune system and ubiquitin supersystem]
    Shigeo Murata
    Tanpakushitsu Kakusan Koso 47:2152-8. 2002
  4. ncbi CHIP: a quality-control E3 ligase collaborating with molecular chaperones
    Shigeo Murata
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, 113 8613, Tokyo, Japan
    Int J Biochem Cell Biol 35:572-8. 2003
  5. ncbi Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes
    Yuko Hirano
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613
    Mol Cell 24:977-84. 2006
  6. ncbi Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Cell 131:1149-63. 2007
  7. pmc PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis
    Katsuhiro Sasaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Setagayaku, Tokyo, Japan
    Mol Cell Biol 30:3864-74. 2010
  8. pmc Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Mol Cell Biol 27:6629-38. 2007
  9. ncbi Loss of autophagy in the central nervous system causes neurodegeneration in mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nature 441:880-4. 2006
  10. pmc A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    EMBO J 25:4524-36. 2006

Collaborators

Detail Information

Publications47

  1. ncbi Regulation of CD8+ T cell development by thymus-specific proteasomes
    Shigeo Murata
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Science 316:1349-53. 2007
    ..Our results suggest a key role for beta5t in generating the MHC class I-restricted CD8(+) T cell repertoire during thymic selection...
  2. ncbi Multiple chaperone-assisted formation of mammalian 20S proteasomes
    Shigeo Murata
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    IUBMB Life 58:344-8. 2006
    ..This article recounts the observations that disclosed the biogenesis of 20S proteasomes and discusses the difference in the mechanism of assembly between archael, yeast, and mammalian 20S proteasomes...
  3. ncbi [Immune system and ubiquitin supersystem]
    Shigeo Murata
    Tanpakushitsu Kakusan Koso 47:2152-8. 2002
  4. ncbi CHIP: a quality-control E3 ligase collaborating with molecular chaperones
    Shigeo Murata
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, 113 8613, Tokyo, Japan
    Int J Biochem Cell Biol 35:572-8. 2003
    ..Accumulating evidence from in vitro studies indicates that this is apparently the case. Here, we present and discuss several unresolved but critical issues related to the molecular mechanism and in vivo roles of CHIP...
  5. ncbi Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes
    Yuko Hirano
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613
    Mol Cell 24:977-84. 2006
    ..Our results describe a cooperative system of multiple chaperones involved in the correct assembly of mammalian 20S proteasomes...
  6. ncbi Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Cell 131:1149-63. 2007
    ....
  7. pmc PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis
    Katsuhiro Sasaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Setagayaku, Tokyo, Japan
    Mol Cell Biol 30:3864-74. 2010
    ..They also accumulated ubiquitinated proteins and exhibited premature senescence. Our results demonstrate the importance of the PAC1-dependent assembly pathway and of the latent 20S proteasomes for maintaining cellular integrity...
  8. pmc Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Mol Cell Biol 27:6629-38. 2007
    ..Our results demonstrate that Rpn10-mediated degradation of ubiquitinated proteins, catalyzed by UIMs, is indispensable for mammalian life...
  9. ncbi Loss of autophagy in the central nervous system causes neurodegeneration in mice
    Masaaki Komatsu
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nature 441:880-4. 2006
    ..Our results indicate that autophagy is essential for the survival of neural cells, and that impairment of autophagy is implicated in the pathogenesis of neurodegenerative disorders involving ubiquitin-containing inclusion bodies...
  10. pmc A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    EMBO J 25:4524-36. 2006
    ..Knockdown of hRpn13 caused no obvious proteolytic defect but loss of UCH37 proteins and decrease in deubiquitinating activity of 26S proteasomes. Our results indicate that hRpn13 is essential for the activity of UCH37...
  11. doi Assembly pathway of the Mammalian proteasome base subcomplex is mediated by multiple specific chaperones
    Takeumi Kaneko
    Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell 137:914-25. 2009
    ..These chaperones dissociate before completion of 26S proteasome formation. Our results demonstrate that base assembly is facilitated by multiple proteasome-dedicated chaperones, like CP assembly...
  12. pmc Dissecting beta-ring assembly pathway of the mammalian 20S proteasome
    Yuko Hirano
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    EMBO J 27:2204-13. 2008
    ..We propose a model in which beta-ring formation is assisted by both intramolecular and extrinsic chaperones, whose roles are partially different between yeast and mammals...
  13. doi Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes
    Hideki Yashiroda
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Struct Mol Biol 15:228-36. 2008
    ..The structure of the Dmp1-Dmp2-alpha5 complex reveals how this chaperone functions in proteasome assembly and why it dissociates from proteasome precursors before the beta-rings are assembled...
  14. doi An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis
    Yoko Kimura
    Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya, Japan
    Cell 137:549-59. 2009
    ..We propose that free ubiquitin chains function as a ubiquitin reservoir that allows maintenance of monomeric ubiquitins at adequate levels under normal conditions and rapid supply for substrate conjugation under stress conditions...
  15. ncbi [The protein quality control and neurodegeneration]
    Keiji Tanaka
    Department of Molecular Oncology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Nihon Yakurigaku Zasshi 122:30-6. 2003
    ..In this article, we discuss the mechanism of neurodegeneration, based on the protein quality control mediated by the ubiquitin-proteasome system in the cell...
  16. doi Molecular mechanisms of proteasome assembly
    Shigeo Murata
    Laboratory of Protein Metabolism, Department of Integrated Biology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nat Rev Mol Cell Biol 10:104-15. 2009
    ..By contrast, little is known about the assembly of the 19S regulatory particles, but several hints have emerged...
  17. pmc Genetic evidence linking age-dependent attenuation of the 26S proteasome with the aging process
    Ayako Tonoki
    Department of Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Mol Cell Biol 29:1095-106. 2009
    ..Our results suggest that maintaining the 26S proteasome with age could extend the life span and suppress the age-related progression of neurodegenerative diseases...
  18. ncbi [Diversity of proteasomes in mammals and its biological significance]
    Shigeo Murata
    Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 73 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Seikagaku 80:719-32. 2008
  19. doi Using siRNA techniques to dissect proteasome assembly pathways in mammalian cells
    Takeumi Kaneko
    Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Methods Mol Biol 832:433-42. 2012
    ..Knockdown of a proteasome subunit causes arrest of the assembly process before incorporation of the targeted subunit and accumulation of a specific intermediate...
  20. ncbi Purification and assay of the chaperone-dependent ubiquitin ligase of the carboxyl terminus of Hsc70-interacting protein
    Shigeo Murata
    Laboratory of Frontier Science, The Tokyo Metropolitan Institute of Medical Science, Precursory Research for Embryonic Science and Technology PRESTO, Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Methods Enzymol 398:271-9. 2005
    ..This chapter describes methods of analyzing chaperone-dependent ubiquitin ligase activity of CHIP using firefly luciferase as a model substrate...
  21. pmc CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response
    Takeshi Maruyama
    Department of Medical Pharmaceutics, Laboratory of Cell Signaling, Japan Science and Technology Corporation, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 29:2501-14. 2010
    ..These findings show that transient activation of the ERK pathway, which depends not only on MEKK2 activation, but also on CHIP-dependent MEKK2 degradation, is crucial for proper gene expression in hyperosmotic stress response...
  22. ncbi [Mechanism of the assembly of mammalian 20S proteasome]
    Yuko Hirano
    Tanpakushitsu Kakusan Koso 51:1230-5. 2006
  23. ncbi [Mechanism of protein degradation by the proteasomes]
    Jun Hamazaki
    Tanpakushitsu Kakusan Koso 51:1213-8. 2006
  24. ncbi A heterodimeric complex that promotes the assembly of mammalian 20S proteasomes
    Yuko Hirano
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Nature 437:1381-5. 2005
    ..Thus, our results identify a mechanism for the correct assembly of 20S proteasomes...
  25. ncbi [Mechanisms of antigen processing]
    Shigeo Murata
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science
    Nihon Rinsho 63:287-92. 2005
  26. ncbi Large- and small-scale purification of mammalian 26S proteasomes
    Yuko Hirano
    Laboratory of Frontier Science The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Methods Enzymol 399:227-40. 2005
    ..However, the details of the ultimate pathophysiological roles of mammalian proteasomes have remained elusive. This article focuses on methods for assay and purification of 26S proteasomes from mammalian cells and tissues...
  27. pmc Keratin 8 is required for the maintenance of architectural structure in thymus epithelium
    Chikako Odaka
    Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 8:e75101. 2013
    ..In addition, the K8/K18 loss affected the distribution of K5/K14-positive mTECs, but not their differentiation status. Together, the results indicate that K8/K18 IFs constitute key promoters of the thymic epithelium integrity. ..
  28. pmc Characterization of the Testis-specific Proteasome Subunit α4s in Mammals
    Hiroyuki Uechi
    From the Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biol Chem 289:12365-74. 2014
    ..4s-containing CPs have a set of peptidase activities almost identical to those of α4-containing CPs. Our results provide a basis for understanding the role of α4s and male germ cell-specific proteasomes in mammals. ..
  29. pmc Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice
    Masaaki Komatsu
    Department of Molecular Oncology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    J Cell Biol 169:425-34. 2005
    ..Our results indicate the important role of autophagy in starvation response and the quality control of proteins and organelles in quiescent cells...
  30. ncbi Allele-selective effect of PA28 in MHC class I antigen processing
    Taketoshi Yamano
    Laboratory for Immunochaperones, Research Center for Allergy and Immunology, RIKEN Yokohama Institute, Tsurumi, Yokohama, Japan
    J Immunol 181:1655-64. 2008
    ....
  31. ncbi Thymoproteasome: probable role in generating positively selecting peptides
    Shigeo Murata
    Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113 0033, Japan
    Curr Opin Immunol 20:192-6. 2008
    ..These findings suggest that cTECs are quite unique cells capable of presenting a unique set of self-peptides that are not seen in other cells and are required for positive selection of CD8(+) T cells...
  32. doi Critical contribution of immunoproteasomes in the induction of protective immunity against Trypanosoma cruzi in mice vaccinated with a plasmid encoding a CTL epitope fused to green fluorescence protein
    Bin Chou
    Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Microbes Infect 10:241-50. 2008
    ....
  33. pmc Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing
    Taketoshi Yamano
    Department of Molecular Medicine, Division of Immunology, Nagasaki University School of Medicine, Nagasaki 852 8523, Japan
    J Exp Med 196:185-96. 2002
    ..Thus, these two pathways operate either redundantly or specifically, depending on antigen species and cell type...
  34. pmc Ligand-dependent switching of ubiquitin-proteasome pathways for estrogen receptor
    Yukiyo Tateishi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan
    EMBO J 23:4813-23. 2004
    ..One pathway is necessary for the transactivation of the receptor and the other is involved in the quality control of the receptor...
  35. ncbi Sterol regulatory element-binding proteins are negatively regulated through SUMO-1 modification independent of the ubiquitin/26 S proteasome pathway
    Yuko Hirano
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113 8657, Japan
    J Biol Chem 278:16809-19. 2003
    ..Considered together, our results indicate that SUMO-1 modification suppresses the transactivation capacity of nuclear SREBPs in a manner different from the negative regulatory mechanism mediated by proteolysis...
  36. pmc A ubiquitin ligase complex assembles linear polyubiquitin chains
    Takayoshi Kirisako
    Department of Molecular Cell Biology, Graduate School of Medicine, Osaka City University, Osaka, Japan
    EMBO J 25:4877-87. 2006
    ..Moreover, the complex regulates the stability of Ub-GFP (a GFP fusion protein with an N-terminal ubiquitin). The linear polyubiquitin chain generated post-translationally may function as a new modulator of proteins...
  37. ncbi The involvement of immunoproteasomes in induction of MHC class I-restricted immunity targeting Toxoplasma SAG1
    Kazunari Ishii
    Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Microbes Infect 8:1045-53. 2006
    ....
  38. ncbi The ubiquitin-proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD8+ T cells
    Xuefeng Duan
    Department of Microbiology and Immunology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Int Immunol 18:679-87. 2006
    ..Thus, application of the ubiquitin-fusion degradation pathway was useful even in immunization with genes encoding a single CTL epitope for induction of specific and active CD8+ T cells...
  39. ncbi Co-chaperone CHIP associates with expanded polyglutamine protein and promotes their degradation by proteasomes
    Nihar Ranjan Jana
    Cellular and Molecular Neuroscience Laboratory, National Brain Research Centre, Manesar, Gurgaon 122 050, India
    J Biol Chem 280:11635-40. 2005
    ..Finally, we show that overexpression of CHIP suppresses the aggregation and cell death mediated by expanded polyglutamine proteins and the suppressive effect is more prominent when CHIP is overexpressed along with Hsc70...
  40. ncbi Formalin-fixed tumor cells effectively induce antitumor immunity both in prophylactic and therapeutic conditions
    Chikage Obata
    Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, 3 1 1, Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    J Dermatol Sci 34:209-19. 2004
    ....
  41. pmc Ubiquitin-fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent protein
    Manxin Zhang
    Department of Microbiology and Immunology, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Immunology 112:567-74. 2004
    ..It is noteworthy to document that genetic immunization with pGFP plus pTRP-2(181-188) failed to exert the antitumour immunity...
  42. pmc Proteasome activator PA28gamma-dependent nuclear retention and degradation of hepatitis C virus core protein
    Kohji Moriishi
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita Shi, Osaka 565 0871, Japan
    J Virol 77:10237-49. 2003
    ..Overexpression of PA28gamma enhanced the proteolysis of the HCV core protein. Thus, the nuclear retention and stability of the HCV core protein is regulated via a PA28gamma-dependent pathway through which HCV pathogenesis may be exerted...
  43. ncbi [The biological significance of the ubiquitin signaling]
    Shigeo Murata
    Nihon Ronen Igakkai Zasshi 41:254-62. 2004
  44. pmc Critical role of PA28gamma in hepatitis C virus-associated steatogenesis and hepatocarcinogenesis
    Kohji Moriishi
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 104:1661-6. 2007
    ..These findings suggest that PA28gamma plays a crucial role in the development of liver pathology induced by HCV infection...
  45. ncbi In vivo evidence of CHIP up-regulation attenuating tau aggregation
    Naruhiko Sahara
    Laboratory for Alzheimer s Disease, RIKEN Brain Science Institute, Saitama, Japan
    J Neurochem 94:1254-63. 2005
    ..If confirmed, this would indicate that the quality-control machinery in a neuron might play an important role in retarding the pathogenesis of tauopathies...
  46. pmc Involvement of the PA28gamma-dependent pathway in insulin resistance induced by hepatitis C virus core protein
    Hironobu Miyamoto
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Virol 81:1727-35. 2007
    ..These results suggest that the HCV core protein suppresses insulin signaling through a PA28gamma-dependent pathway...
  47. ncbi Dorfin ubiquitylates mutant SOD1 and prevents mutant SOD1-mediated neurotoxicity
    Jun ichi Niwa
    Department of Neurology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya 466 8550, Japan
    J Biol Chem 277:36793-8. 2002
    ..Our results indicate that Dorfin protects neurons by recognizing and then ubiquitylating mutant SOD1 proteins followed by targeting them for proteasomal degradation...