Tadashi Matsunag

Summary

Affiliation: Tokyo University of Agriculture and Technology
Country: Japan

Publications

  1. ncbi request reprint Screening of marine microalgae for bioremediation of cadmium-polluted seawater
    T Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan
    J Biotechnol 70:33-8. 1999
  2. ncbi request reprint Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology Koganei, Tokyo, Japan
    DNA Res 12:157-66. 2005
  3. ncbi request reprint Genes and proteins involved in bacterial magnetic particle formation
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Trends Microbiol 11:536-41. 2003
  4. ncbi request reprint Marine microalgae
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184 8588 Tokyo, Japan
    Adv Biochem Eng Biotechnol 96:165-88. 2005
  5. ncbi request reprint Magnetic separation of CD14+ cells using antibody binding with protein A expressed on bacterial magnetic particles for generating dendritic cells
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biochem Biophys Res Commun 350:1019-25. 2006
  6. ncbi request reprint High-throughput SNP detection using nano-scale engineered biomagnetite
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 22:2315-21. 2007
  7. ncbi request reprint Molecular analysis of magnetotactic bacteria and development of functional bacterial magnetic particles for nano-biotechnology
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Trends Biotechnol 25:182-8. 2007
  8. ncbi request reprint Fully automated immunoassay for detection of prostate-specific antigen using nano-magnetic beads and micro-polystyrene bead composites, 'Beads on Beads'
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Anal Chim Acta 597:331-9. 2007
  9. doi request reprint High-efficiency single-cell entrapment and fluorescence in situ hybridization analysis using a poly(dimethylsiloxane) microfluidic device integrated with a black poly(ethylene terephthalate) micromesh
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Anal Chem 80:5139-45. 2008
  10. ncbi request reprint Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 77:614-8. 2002

Collaborators

Detail Information

Publications83

  1. ncbi request reprint Screening of marine microalgae for bioremediation of cadmium-polluted seawater
    T Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan
    J Biotechnol 70:33-8. 1999
    ..The Cd removal by freeze-dried NKG16014 cells was also determined. Cd was more quickly adsorbed by dried cells than that by living cells, with a qmax of 91.0 mg Cd (g dry cells)-1...
  2. ncbi request reprint Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology Koganei, Tokyo, Japan
    DNA Res 12:157-66. 2005
    ....
  3. ncbi request reprint Genes and proteins involved in bacterial magnetic particle formation
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Trends Microbiol 11:536-41. 2003
    ....
  4. ncbi request reprint Marine microalgae
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184 8588 Tokyo, Japan
    Adv Biochem Eng Biotechnol 96:165-88. 2005
    ..The diversified marine environment harbors a large variety of microalgae. In this paper, the biotechnological aspects and fundamental characteristics of marine microalgae are reviewed...
  5. ncbi request reprint Magnetic separation of CD14+ cells using antibody binding with protein A expressed on bacterial magnetic particles for generating dendritic cells
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biochem Biophys Res Commun 350:1019-25. 2006
    ..Furthermore, in the evaluation of the influence of protein A-BacMPs on the separated cells, the CD14(+) cells separated using protein A-BacMPs and were successfully differentiated into dendritic cells...
  6. ncbi request reprint High-throughput SNP detection using nano-scale engineered biomagnetite
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 22:2315-21. 2007
    ..High reliability for the semi-automated BacMP-based SNP detection system was confirmed following comparison to traditional sequencing-based methods...
  7. ncbi request reprint Molecular analysis of magnetotactic bacteria and development of functional bacterial magnetic particles for nano-biotechnology
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Trends Biotechnol 25:182-8. 2007
    ..This review describes the recent advances in the fundamental analysis of bacterial magnetic particles and the development of surface-protein-modified magnetic particles for biotechnological applications...
  8. ncbi request reprint Fully automated immunoassay for detection of prostate-specific antigen using nano-magnetic beads and micro-polystyrene bead composites, 'Beads on Beads'
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Anal Chim Acta 597:331-9. 2007
    ..48 ng mL(-1). From this preliminary assay, it can be seen that 'Beads on Beads' could be a powerful tool in the development of high-throughput, fully automated multiplexed bioassays...
  9. doi request reprint High-efficiency single-cell entrapment and fluorescence in situ hybridization analysis using a poly(dimethylsiloxane) microfluidic device integrated with a black poly(ethylene terephthalate) micromesh
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Anal Chem 80:5139-45. 2008
    ..Differences in the levels of beta-actin mRNA expression were observed in serum-supplied or serum-starved cell populations...
  10. ncbi request reprint Preparation of luciferase-bacterial magnetic particle complex by artificial integration of MagA-luciferase fusion protein into the bacterial magnetic particle membrane
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 77:614-8. 2002
    ..Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques...
  11. ncbi request reprint Fluorescent detection of cyanobacterial DNA using bacterial magnetic particles on a MAG-microarray
    T Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 73:400-5. 2001
    ..The entire process of hybridization and detection was automatically performed using a magnetic-separation robot and all five cyanobacterial genera were successfully discriminated...
  12. ncbi request reprint Microaerobic hydrogen production by photosynthetic bacteria in a double-phase photobioreactor
    T Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 68:647-51. 2000
    ..Hydrogen production using a double-phase photobioreactor was demonstrated continuously at the same rate for 150 h...
  13. ncbi request reprint Cloning and characterization of a gene, mpsA, encoding a protein associated with intracellular magnetic particles from Magnetospirillum sp. strain AMB-1
    T Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Koganei, Tokyo, 184 8588, Japan
    Biochem Biophys Res Commun 268:932-7. 2000
    ..Although the function of MpsA remains unknown, homology searches suggest similarity with the alpha subunit of acetyl-CoA carboxylase and the CoA-binding motif...
  14. doi request reprint Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production
    Tadashi Matsunaga
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Lett 31:1367-72. 2009
    ..The strain JPCC GA0024, S. rubescens, will become a promising resource that can grow as a dominant species in the seawater for the production of both liquid and solid biofuels...
  15. ncbi request reprint Bioengineering of bacterial magnetic particles and their applications in biotechnology
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo, 184 8588, Japan
    Recent Pat Biotechnol 4:214-25. 2010
    ..The elucidation of the mechanism of magnetic particle synthesis has provided a roadmap for the design of novel biomaterials that can play useful roles in multiple disciplinary fields...
  16. pmc Efficient and stable display of functional proteins on bacterial magnetic particles using mms13 as a novel anchor molecule
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 72:465-71. 2006
    ..The results of this study demonstrated the utility of Mms13 as a molecular anchor, which will facilitate the assembly of other functional proteins onto BMPs in the near feature...
  17. doi request reprint Magnetic cell separation using nano-sized bacterial magnetic particles with reconstructed magnetosome membrane
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 101:470-7. 2008
    ..Magnetic separation of monocytes and B-lymphocytes from the peripheral blood was achieved with high purity using magnetosome membrane-reconstructed protein A-BacMPs...
  18. ncbi request reprint Single nucleotide polymorphism genotyping of aldehyde dehydrogenase 2 gene using a single bacterial magnetic particle
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, 184 8588, Tokyo, Japan
    Biosens Bioelectron 18:661-6. 2003
    ..SNP genotyping of ALDH2 has been successfully achieved at a single particle level using BMP...
  19. doi request reprint Single-cell detection using a thin film transistor photosensor with micro-partitions
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Lab Chip 10:3348-54. 2010
    ..This system will allow high-throughput and real-time analysis of more than 10(4) cells with minimum optical system requirements...
  20. ncbi request reprint Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy
    Yosuke Amemiya
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho Koganei, Tokyo 184 8588, Japan
    J Biotechnol 120:308-14. 2005
    ..This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities...
  21. pmc Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1
    Takeyuki Suzuki
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo, Japan 184 8588
    J Bacteriol 188:2275-9. 2006
    ..The concomitant rapid iron uptake and magnetite formation suggest that these uptake systems serve as iron supply lines for magnetosome synthesis...
  22. ncbi request reprint Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle
    Hiroyuki Ota
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biomol Eng 20:305-9. 2003
    ..Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs...
  23. doi request reprint Novel method for evaluation of chemicals based on ligand-dependent recruitment of GFP labeled coactivator to estrogen receptor displayed on bacterial magnetic particles
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Anal Chim Acta 626:71-7. 2008
    ..Finally, this method is amenable adaptation into a high throughput format by using automated magnetic separation...
  24. pmc Inducible expression of transmembrane proteins on bacterial magnetic particles in Magnetospirillum magneticum AMB-1
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:1152-7. 2010
    ..These results suggest that the tetracycline-inducible expression system described here will be a useful tool for the expression and display of transmembrane proteins in the membranes of BacMPs...
  25. ncbi request reprint [Protein display onto nano-sized bacterial magnetic particles for receptor analysis]
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan
    Yakugaku Zasshi 129:1319-25. 2009
    ..The elucidation of the mechanism of BacMP synthesis has provided a roadmap for the design of novel nano-biomaterials that would play a useful role in multidisciplinary fields...
  26. ncbi request reprint Single nucleotide polymorphism detection in aldehyde dehydrogenase 2 (ALDH2) gene using bacterial magnetic particles based on dissociation curve analysis
    Kohei Maruyama
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, 184 8588, Japan
    Biotechnol Bioeng 87:687-94. 2004
    ..In the heterozygote target DNA (ALDH2*1/*2), each fluorescence signals was at a similar level. Therefore, three genotypes of SNP in ALDH2 gene were detected using the automated detection system with BMPs...
  27. ncbi request reprint Spontaneous integration of transmembrane peptides into a bacterial magnetic particle membrane and its application to display of useful proteins
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo, Japan
    Anal Chem 76:3764-9. 2004
    ..The present study shows that temporin L is a stable molecular anchor on BMPs by the binding of soluble protein to the N-terminus...
  28. ncbi request reprint Single-nucleotide polymorphism analysis using fluorescence resonance energy transfer between DNA-labeling fluorophore, fluorescein isothiocyanate, and DNA intercalator, POPO-3, on bacterial magnetic particles
    Hideki Nakayama
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 84:96-102. 2003
    ..The assay described herein allows for a simple and rapid SNP analysis using a fully automated system...
  29. ncbi request reprint SNP detection in transforming growth factor-beta1 gene using bacterial magnetic particles
    Hiroyuki Ota
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, 184 8588, Tokyo, Japan
    Biosens Bioelectron 18:683-7. 2003
    ..Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique...
  30. pmc Preparation of genomic DNA from a single species of uncultured magnetotactic bacterium by multiple-displacement amplification
    Atsushi Arakaki
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:1480-5. 2010
    ..The combined use of magnetic separation, flow cytometry, and MDA provides a new strategy to access individual genetic information from magnetotactic bacteria in environmental samples...
  31. doi request reprint Identification and functional characterization of liposome tubulation protein from magnetotactic bacteria
    Masayoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Mol Microbiol 76:480-8. 2010
    ....
  32. pmc Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 74:7600-6. 2008
    ..This BacMP-based method could be a promising approach for a high-throughput screening of antimicrobial peptides targeting a wide range of species...
  33. ncbi request reprint Development of efficient expression system for protein display on bacterial magnetic particles
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biochem Biophys Res Commun 338:1678-81. 2005
    ..This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles...
  34. pmc Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mms16 as an anchor molecule
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 70:2880-5. 2004
    ..After washing, the complexes were ready to use for analysis. This system conveniently refines the native conformation of GPCRs without the need for detergent solubilization, purification, and reconstitution after cell disruption...
  35. ncbi request reprint Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms
    Takahito Nakagawa
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 94:862-8. 2006
    ..DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated...
  36. pmc In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide
    Yoshiaki Maeda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 76:5785-90. 2010
    ..coli biotin ligase. This study describes not only a simple method to produce biotinylated nanomagnetic particles but also a possible expansion of in vivo biotinylation technology for bacterial investigation...
  37. ncbi request reprint Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1
    Takeyuki Suzuki
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    FEBS Lett 581:3443-8. 2007
    ..This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1...
  38. ncbi request reprint Origin of magnetosome membrane: proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane
    Masayoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan
    Proteomics 6:5234-47. 2006
    ..Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM...
  39. ncbi request reprint Saccharification of marine microalgae using marine bacteria for ethanol production
    Mitsufumi Matsumoto
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 26, Naka cho, Koganei, Tokyo, Japan
    Appl Biochem Biotechnol 105:247-54. 2003
    ..Terrestrial amylase and glucoamylase were inactive in saline suspension. Therefore, marine amylase is necessary in saline conditions for successful saccharification of marine microalgae...
  40. doi request reprint Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood
    Keiichi Hatakeyama
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, 184 8588 Tokyo, Japan
    Lab Chip 9:1052-8. 2009
    ..The assay was completed in less than one hour. Our technology will be a promising approach to developing a miniaturized, disposable DNA chip with high sensitivity...
  41. doi request reprint Direct magnetic separation of immune cells from whole blood using bacterial magnetic particles displaying protein G
    Masayuki Takahashi
    Dept of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Prog 25:219-26. 2009
    ..The results of this study demonstrate the utility of protein G-BacMP and this particle may become a powerful tool for various therapeutic or diagnosis applications...
  42. pmc Noncovalent immobilization of streptavidin on in vitro- and in vivo-biotinylated bacterial magnetic particles
    Yoshiaki Maeda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 74:5139-45. 2008
    ..By combining the site-selective biotinylation technology and the protein display technology, more innovative and attractive magnetic nanomaterials can be constructed...
  43. ncbi request reprint DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound
    Brandon Yoza
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Koganei, Tokyo 184 8588, Japan
    J Biotechnol 94:217-24. 2002
    ..Ten replicate samples of E. coli cells were extracted using an automated magnetic robot and 9.5 microg of DNA was extracted using 100 microg of modified bacterial magnetite and possessed a 1.94 absorbance ratio (260:280 nm)...
  44. pmc Development of a cell surface display system in a magnetotactic bacterium, "Magnetospirillum magneticum" AMB-1
    Masayoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Appl Environ Microbiol 74:3342-8. 2008
    ..The established AMB-1 transformant showed high immunofluorescence response, high Cd(2+) binding, and high recovery efficiency in comparison to those of the negative control when manipulated by magnetic force...
  45. ncbi request reprint Controlled formation of magnetite crystal by partial oxidation of ferrous hydroxide in the presence of recombinant magnetotactic bacterial protein Mms6
    Yosuke Amemiya
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho Koganei, Tokyo 184 8588, Japan
    Biomaterials 28:5381-9. 2007
    ..The method introduced in this study presents an alternative route for controlling the size and shape of magnetite crystals without the use of organic solvent and high temperatures...
  46. ncbi request reprint Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1
    Aris Tri Wahyudi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biochem Biophys Res Commun 303:223-9. 2003
    ..These results indicate that AOR may contribute to ferric iron reduction during BMP synthesis in M. magneticum AMB-1 under microaerobic respiration...
  47. doi request reprint A stable human progesterone receptor expressing HeLa reporter cell line as a tool in chemical evaluation at the different cell-cycle phases
    Tetsushi Mori
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Toxicol Lett 186:123-9. 2009
    ....
  48. ncbi request reprint DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer
    Brandon Yoza
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Koganei, Japan
    J Biotechnol 101:219-28. 2003
    ....
  49. ncbi request reprint Electrochemical probe for on-chip type flow immunoassay: immunoglobulin G labeled with ferrocenecarboaldehyde
    Mina Okochi
    Department of Biotechnology and Life Sciences, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 90:14-9. 2005
    ..Ag/AgCl. The minimum detectable concentration of IgG labeled with Fc-CHO was 0.06 microM. IgG labeled with Fc-CHO demonstrate biochemical and electrochemical properties that are useful for electrochemical immunosensors...
  50. ncbi request reprint Siderophore production of a periplasmic transport binding protein kinase gene defective mutant of Magnetospirillum magneticum AMB-1
    Ronie J Calugay
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biochem Biophys Res Commun 323:852-7. 2004
    ..These data suggest that the interrupted periplasmic transport binding protein kinase gene homologue is required for siderophore transport into M. magneticum AMB-1...
  51. doi request reprint Surface modification of magnetic nanoparticles using asparagines-serine polypeptide designed to control interactions with cell surfaces
    Masayuki Takahashi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biomaterials 31:4952-7. 2010
    ..This technology, incorporating a functional polypeptide, may represent a completely new strategy for surface modification of nanoparticles for use in a variety of cell-associated applications...
  52. ncbi request reprint A novel protein tightly bound to bacterial magnetic particles in Magnetospirillum magneticum strain AMB-1
    Atsushi Arakaki
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan
    J Biol Chem 278:8745-50. 2003
    ..These proteins may be directly involved in biological magnetite crystal formation in magnetic bacteria...
  53. ncbi request reprint Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 19:325-30. 2003
    ..This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs...
  54. ncbi request reprint Dynamic analysis of a genomic island in Magnetospirillum sp. strain AMB-1 reveals how magnetosome synthesis developed
    Yorikane Fukuda
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Koganei, Tokyo 184 8588, Japan
    FEBS Lett 580:801-12. 2006
    ....
  55. ncbi request reprint Desulfovibrio magneticus sp. nov., a novel sulfate-reducing bacterium that produces intracellular single-domain-sized magnetite particles
    Toshifumi Sakaguchi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan
    Int J Syst Evol Microbiol 52:215-21. 2002
    ..A novel species, Desulfovibrio magneticus sp. nov., is proposed for RS-1T (= ATCC 700980T = DSM 13731T), a sulfate-reducing magnetotactic bacterium...
  56. pmc Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum
    Yoshiko Okamura
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Koganei, Tokyo
    Appl Environ Microbiol 69:4274-7. 2003
    ..An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed...
  57. ncbi request reprint Oligonucleotide-arrayed TFT photosensor applicable for DNA chip technology
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 95:22-8. 2006
    ..Use of the TFT photosensor will allow the development of a disposable photodetecting device for DNA chip systems...
  58. pmc Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria
    Hidekazu Nakazawa
    NITE Genome Analysis Center, Department of Biotechnology, National Institute of Technology and Evaluation, Shibuya ku, Tokyo, Japan
    Genome Res 19:1801-8. 2009
    ..Our findings suggest the presence of core genetic components for magnetosome biosynthesis; these genes may have been acquired into the magnetotactic bacterial genomes by multiple gene-transfer events during proteobacterial evolution...
  59. pmc Formation of magnetite by bacteria and its application
    Atsushi Arakaki
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo 184 8588, Japan
    J R Soc Interface 5:977-99. 2008
    ..The nano-sized fine magnetic particles offer vast potential in new nano-techniques...
  60. doi request reprint Magnetic separation of melanoma-specific cytotoxic T lymphocytes from a vaccinated melanoma patient's blood using MHC/peptide complex-conjugated bacterial magnetic particles
    Masayuki Takahashi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Bioconjug Chem 20:304-9. 2009
    ..The potential of MHC/peptide complex-conjugated BacMPs was indicated for efficient separation of antigen-specific CTLs in cancer immunotherapy and fundamental research...
  61. doi request reprint Nano-sized bacterial magnetic particles displaying pyruvate phosphate dikinase for pyrosequencing
    Tomoko Yoshino
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 103:130-7. 2009
    ..The recycling of enzymes was also shown to be promising and applicable for the development of an inexpensive DNA sequencing at a low running cost...
  62. ncbi request reprint Miniaturized amperometric flow immunoassay system using a glass fiber membrane modified with anion
    Tae Kyu Lim
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 77:758-63. 2002
    ..This miniaturized amperometric flow immunoassay requires only minute quantities of serum and generates highly reproducible results...
  63. doi request reprint Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Naka cho, Koganei, Tokyo 184 8588, Japan
    Langmuir 25:2956-61. 2009
    ....
  64. ncbi request reprint Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1
    Ronie J Calugay
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo, Japan
    FEMS Microbiol Lett 218:371-5. 2003
    ..magneticum AMB-1 for the assimilation of large amounts of iron accounts for the rapid depletion of iron in the medium, thereby triggering siderophore excretion. M. magneticum AMB-1 produces both hydroxamate and catechol siderophores...
  65. ncbi request reprint Catechol siderophore excretion by magnetotactic bacterium Magnetospirillum magneticum AMB-1
    Ronie J Calugay
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan
    J Biosci Bioeng 101:445-7. 2006
    ..The structure of the purified compound was 3,4-dihydroxybenzoic acid as determined by nuclear magnetic resonance (NMR) and electro-spray ionization mass spectroscopy (ESI-MS)...
  66. ncbi request reprint Whole-metagenome amplification of a microbial community associated with scleractinian coral by multiple displacement amplification using phi29 polymerase
    Hiroko Yokouchi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Environ Microbiol 8:1155-63. 2006
    ..This study demonstrates that MDA techniques are efficient for genome wide investigation to understand the actual microbial diversity in limited bacterial samples...
  67. ncbi request reprint Microfabricated on-chip-type electrochemical flow immunoassay system for the detection of histamine released in whole blood samples
    Tae Kyu Lim
    Department of Biotechnology and Life Sciences, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Anal Chem 75:3316-21. 2003
    ..This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results...
  68. ncbi request reprint Determination of microsatellite repeats in the human thyroid peroxidase (TPOX) gene using an automated gene analysis system with nanoscale engineered biomagnetite
    Takahito Nakagawa
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16, Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 22:2276-81. 2007
    ..Furthermore, rapid determination of repeat numbers was possible by measuring fluorescence intensities obtained by probe dissociation at 56 and 66 degrees C, and 40, 60 and 80 degrees C for signal normalization...
  69. ncbi request reprint Magnetic cell separation using antibody binding with protein a expressed on bacterial magnetic particles
    Motoki Kuhara
    Technical and Development, Medical and Biological Laboratories Company Ltd, 1063 103, Ohara, Terasawaoka, Ina, Nagano, 396 0002, Japan
    Anal Chem 76:6207-13. 2004
    ....
  70. ncbi request reprint Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles
    Atsushi Arakaki
    Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3 4 1, Shinjuku ku, Tokyo 169 8555, Japan
    Biotechnol Bioeng 88:543-6. 2004
    ..The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses...
  71. ncbi request reprint Immuno-capture of Cryptosporidium parvum using micro-well array
    Tomoyuki Taguchi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 20:2276-82. 2005
    ..parvum oocysts were stably captured onto the chip after repeated washing procedures. These data show that the newly designed micro-well array technique described here is useful for antibody-mediated C. parvum capture...
  72. ncbi request reprint Synthesis of magnetic nanoparticles and their application to bioassays
    Tetsuya Osaka
    Department of Applied Chemistry, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo, 169 8555, Japan
    Anal Bioanal Chem 384:593-600. 2006
    ....
  73. ncbi request reprint Shc family expression in neuroblastoma: high expression of shcC is associated with a poor prognosis in advanced neuroblastoma
    Elena Terui
    Department of Pediatric Surgery E6, Graduate School of Medicine, Chiba University, Chiba, Japan
    Clin Cancer Res 11:3280-7. 2005
    ..Our results suggest that the expressions of shcB and shcC are important biological factors in neuroblastoma and are useful prognostic indicators...
  74. ncbi request reprint Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles
    Hiroyuki Ota
    Malcom Co, Ltd, 4 15 10 Honmachi Shibuya ku, Tokyo 151 0071, Japan
    J Biotechnol 125:361-8. 2006
    ....
  75. ncbi request reprint Development of an electrochemical antifouling system for seawater cooling pipelines of power plants using titanium
    Hitoshi Wake
    Central Research Laboratory, Pentel Co Ltd, Soka, Saitama, Japan
    Biotechnol Bioeng 95:468-73. 2006
    ..Using titanium as the electrode material, chlorine and hypochlorite are not generated. The developed electrochemical antifouling system provided an effective, environmentally friendly, and feasible techniques for remote operations...
  76. ncbi request reprint Recent developments in laboratory automation using magnetic particles for genome analysis
    Kimimichi Obata
    Precision System Science Co Ltd, 88 Kamihongo, Matsudo, Chiba, 271 0064, Japan
    Pharmacogenomics 3:697-708. 2002
    ..Methods using magnetic particles, which have been used for the automation of nucleic acid purification and immunoassay, appear to be the most promising way to automate processes used in biological research...
  77. ncbi request reprint Astaxanthin formation in the marine photosynthetic bacterium Rhodovulum sulfidophilum expressing crtI, crtY, crtW and crtZ
    Daikichi Mukoyama
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan
    FEMS Microbiol Lett 265:69-75. 2006
    ..longus crtI and crtY genes. A transconjugant, which can synthesize astaxanthin, was successfully generated (2.0 microg g(-1) DCW)...
  78. ncbi request reprint Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite
    Kohei Maruyama
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biosens Bioelectron 22:2282-8. 2007
    ..5h using this system, suggesting that this system could be used in clinical tests of EGFR gene mutations in lung cancer, and potentially other cancer, patients. Additionally, a very low mutation rate could be detected in these samples...
  79. ncbi request reprint Reciprocal expression of CCAAT/enhancer binding proteins alpha and beta in hepatoblastomas and its prognostic significance
    Minoru Tomizawa
    Department of Medicine and Clinical Oncology, Chiba University Graduate School of Medicine, Chiba 260 8670, Japan
    Oncol Rep 17:341-4. 2007
    ..Thus, expression of C/EBP proteins may play an important role in the genesis and clinical behavior of hepatoblastoma probably by inducing different stages of arrest of differentiation...
  80. ncbi request reprint In situ identification of symbiotic dinoflagellates, the genus Symbiodinium with fluorescence-labeled rRNA-targeted oligonucleotide probes
    Hiroko Yokouchi
    Department of Biotechnology, Tokyo University of Agriculture and Technology 2 24 16, Naka cho, Koganei, Japan
    J Microbiol Methods 53:327-34. 2003
    ..Symbiodinium isolated from jellyfish Cassiopea sp. and sea anemone Aiptasia sp. were classified as belonging to clades A and B using the FISH procedure established in this study...
  81. ncbi request reprint Preparation of a colored conductive paint electrode for electrochemical inactivation of bacteria
    Tae Kyu Lim
    Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184 8588, Japan
    Biotechnol Bioeng 81:299-304. 2003
    ..Chlorine was not detected below an applied potential of 1.5 V. A change in pH was not observed in the range of 0 to 1.5 V. This method might be effective for preventing bacterial cell accumulation and the formation of biofilms...
  82. ncbi request reprint Direct counting of Cryptosporidium parvum oocysts using fluorescence in situ hybridization on a membrane filter
    Tomoyuki Taguchi
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    J Microbiol Methods 67:373-80. 2006
    ....
  83. ncbi request reprint Rapid and sensitive detection of 17beta-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles
    Tsuyoshi Tanaka
    Department of Biotechnology, Tokyo University of Agriculture and Technology, 2 24 16 Naka cho, Koganei, Tokyo 184 8588, Japan
    J Biotechnol 108:153-9. 2004
    ..This system will enable us to determine low E2 concentrations without sample condensation...