Hisao Masai

Summary

Affiliation: Tokyo Metropolitan Institute of Medical Science
Country: Japan

Publications

  1. ncbi request reprint Cdc7 kinase complex: a key regulator in the initiation of DNA replication
    Hisao Masai
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo, Japan
    J Cell Physiol 190:287-96. 2002
  2. ncbi request reprint Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin
    Hisao Masai
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 281:39249-61. 2006
  3. ncbi request reprint Control of DNA replication: regulation and activation of eukaryotic replicative helicase, MCM
    Hisao Masai
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    IUBMB Life 57:323-35. 2005
  4. ncbi request reprint A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases
    Naoko Yoshizawa-Sugata
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 280:13062-70. 2005
  5. pmc Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation
    Seiji Matsumoto
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8613, Japan
    J Cell Biol 195:387-401. 2011
  6. ncbi request reprint Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex
    Seiji Matsumoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 280:42536-42. 2005
  7. pmc Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast
    Seiji Matsumoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Cell Cycle 9:4627-37. 2010
  8. pmc Roles of human AND-1 in chromosome transactions in S phase
    Naoko Yoshizawa-Sugata
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 284:20718-28. 2009
  9. pmc Hsk1 kinase is required for induction of meiotic dsDNA breaks without involving checkpoint kinases in fission yeast
    Keiko Ogino
    Genome Dynamics Project and Department of Integrated Life Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Proc Natl Acad Sci U S A 103:8131-6. 2006
  10. ncbi request reprint Functional analyses of mouse ASK, an activation subunit for Cdc7 kinase, using conditional ASK knockout ES cells
    Nobuyuki Yamashita
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Genes Cells 10:551-63. 2005

Collaborators

Detail Information

Publications57

  1. ncbi request reprint Cdc7 kinase complex: a key regulator in the initiation of DNA replication
    Hisao Masai
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo, Japan
    J Cell Physiol 190:287-96. 2002
    ..The interplay between Cdc7 and Cdk, another kinase essential for the S phase, is also discussed...
  2. ncbi request reprint Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin
    Hisao Masai
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 281:39249-61. 2006
    ..These results are consistent with the notion that the N-terminal phosphorylation of MCM2, MCM4, and MCM6 may play functionally redundant but essential roles in initiation of DNA replication...
  3. ncbi request reprint Control of DNA replication: regulation and activation of eukaryotic replicative helicase, MCM
    Hisao Masai
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    IUBMB Life 57:323-35. 2005
    ....
  4. ncbi request reprint A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases
    Naoko Yoshizawa-Sugata
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 280:13062-70. 2005
    ..Furthermore, mitotic progression is retarded in ASKL1 or Cdc7 siRNA-treated cells. Our results suggest that ASKL1 in a complex with Cdc7 may play a role in normal progression of both S and M phases...
  5. pmc Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation
    Seiji Matsumoto
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8613, Japan
    J Cell Biol 195:387-401. 2011
    ....
  6. ncbi request reprint Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex
    Seiji Matsumoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 280:42536-42. 2005
    ..These data suggest that Hsk1-Dfp1/Him1 and Swi1-Swi3 complexes have interrelated roles in stabilization of arrested replication forks...
  7. pmc Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast
    Seiji Matsumoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Cell Cycle 9:4627-37. 2010
    ..The results suggest that Hsk1-mediated loading of Cdc45 onto replication origins may play important roles in replication stress-induced checkpoint...
  8. pmc Roles of human AND-1 in chromosome transactions in S phase
    Naoko Yoshizawa-Sugata
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 284:20718-28. 2009
    ....
  9. pmc Hsk1 kinase is required for induction of meiotic dsDNA breaks without involving checkpoint kinases in fission yeast
    Keiko Ogino
    Genome Dynamics Project and Department of Integrated Life Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    Proc Natl Acad Sci U S A 103:8131-6. 2006
    ..These results indicate unique and essential roles of Hsk1 kinase in the initiation of meiotic recombination and meiosis...
  10. ncbi request reprint Functional analyses of mouse ASK, an activation subunit for Cdc7 kinase, using conditional ASK knockout ES cells
    Nobuyuki Yamashita
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Genes Cells 10:551-63. 2005
    ..These results may suggest that motif-N of ASK may facilitate recruitment of substrates for Cdc7 kinase...
  11. pmc Rif1 regulates the replication timing domains on the human genome
    Satoshi Yamazaki
    Genome Dynamics Project, Department of Genome Medicine, Tokyo, Japan
    EMBO J 31:3667-77. 2012
    ..Our results indicate that Rif1 plays crucial roles in determining the replication timing domain structures in human cells through regulating higher-order chromatin architecture...
  12. pmc Mechanism of cancer cell death induced by depletion of an essential replication regulator
    Sayuri Ito
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo, Japan
    PLoS ONE 7:e36372. 2012
    ..Depletion of Cdc7, a kinase essential for initiation of DNA replication, induces cancer cell death regardless of its p53 status, but the precise pathways of cell death induction have not been characterized...
  13. pmc Rif1 is a global regulator of timing of replication origin firing in fission yeast
    Motoshi Hayano
    Genome Dynamics, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo, Japan
    Genes Dev 26:137-50. 2012
    ..Our data demonstrate that Rif1 is a critical determinant of the origin activation program on the fission yeast chromosomes...
  14. pmc Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast
    Michie Shimmoto
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    Genes Cells 14:669-82. 2009
    ..These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast...
  15. ncbi request reprint Escherichia coli PriA protein, two modes of DNA binding and activation of ATP hydrolysis
    Taku Tanaka
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 282:19917-27. 2007
    ..We propose architecture of PriA bound to various arrested replication fork structures and discuss its implication in helicase activation and ATP hydrolysis...
  16. pmc Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1
    Taku Tanaka
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 285:39609-22. 2010
    ..Our results reveal an aspect of molecular interactions that may play an important role in replication pausing and fork stabilization...
  17. ncbi request reprint Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved noncoding sequence
    Toshiro Hayashida
    Cytokine Project, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, 113 8613 Tokyo, Japan
    J Immunol 176:5446-54. 2006
    ....
  18. pmc Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognition
    Ryo Kitamura
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 286:23031-43. 2011
    ..These results indicate bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase...
  19. ncbi request reprint Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint
    Naoko Yoshizawa-Sugata
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 282:2729-40. 2007
    ..Our results indicate that mammalian Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin in response to replication checkpoint...
  20. pmc Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast
    Motoshi Hayano
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa 2 1 6, Setagaya Ku, Tokyo 156 8506, Japan
    Mol Cell Biol 31:2380-91. 2011
    ..We propose that prefiring binding of Mrc1 is an important marker of early-firing origins which are precociously activated by the absence of this protein...
  21. pmc Cdt1 forms a complex with the minichromosome maintenance protein (MCM) and activates its helicase activity
    Zhiying You
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
    J Biol Chem 283:24469-77. 2008
    ..Thus, a productive interaction between Cdt1 and MCM appears to be essential for efficient loading of MCM onto template DNA, as well as for the efficient unwinding reaction...
  22. ncbi request reprint Stabilization of a stalled replication fork by concerted actions of two helicases
    Taku Tanaka
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 281:3484-93. 2006
    ..Our results present a novel mechanism by which two helicases function in a highly coordinated manner to generate a structure in which an arrested fork is stabilized for further repair and/or replication restart...
  23. doi request reprint Identification of stimulators and inhibitors of Cdc7 kinase in vitro
    Naoko Kakusho
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 283:19211-8. 2008
    ....
  24. ncbi request reprint ATPase/helicase motif mutants of Escherichia coli PriA protein essential for recombination-dependent DNA replication
    Taku Tanaka
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Genes Cells 8:251-61. 2003
    ..However, the roles of ATPase/DNA helicase activities in functions of PriA are not well understood...
  25. ncbi request reprint Cell cycle regulation of chromatin binding and nuclear localization of human Cdc7-ASK kinase complex
    Noriko Sato
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Genes Cells 8:451-63. 2003
    ..In mammals, it is not known at which time point during the cell cycle Cdc7 and Dbf4/ASK proteins are imported into nuclei and loaded on to chromatin...
  26. ncbi request reprint Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex
    Zhiying You
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
    J Biol Chem 277:42471-9. 2002
    ..Komamura, Y., and Ishimi, Y. (1999) Mol. Cell. Biol. 19, 8003-8015), our data indicate distinct roles of Mcm4, Mcm6, and Mcm7 subunits in activation of the DNA helicase activity of the Mcm4/6/7 complex...
  27. ncbi request reprint Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication. Mei4-dependent transcription as a potential target of meiotic checkpoint
    Keiko Ogino
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 281:1338-44. 2006
    ....
  28. ncbi request reprint Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells
    Hiroko Fujii-Yamamoto
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    J Biol Chem 280:12976-87. 2005
    ..Furthermore, they are rapidly down-regulated upon induction of differentiation of ES cells. The significance of these findings is discussed in relation to the unusual proliferative properties of ES cells in an undifferentiated state...
  29. pmc DNA binding and helicase actions of mouse MCM4/6/7 helicase
    Zhiying You
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science 18 22, Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
    Nucleic Acids Res 33:3033-47. 2005
    ..Taken together, these findings reveal important features on activation and substrate preference of the eukaryotic replicative helicase...
  30. pmc Thymine-rich single-stranded DNA activates Mcm4/6/7 helicase on Y-fork and bubble-like substrates
    Zhiying You
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
    EMBO J 22:6148-60. 2003
    ..These findings lead us to propose that selective activation by stretches of thymine sequences of a fraction of Mcm helicases loaded onto chromatin may be the determinant for selection of initiation sites on mammalian genomes...
  31. ncbi request reprint Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and development
    Jung Min Kim
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Mutat Res 532:29-40. 2003
    ..Partial, rather than total, loss of Cdc7 kinase expression results in retarded growth at both cellular and whole body levels, with especially profound impairment of germ cell development...
  32. pmc Inhibition of DNA damage-induced apoptosis through Cdc7-mediated stabilization of Tob
    Toru Suzuki
    Department of Cancer Biology, Division of Oncology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Tokyo 108 8639, Japan
    J Biol Chem 287:40256-65. 2012
    ..Preventing unnecessary cell death is essential for DNA-damaged cells to carry out the DNA repair process...
  33. pmc Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1
    Kenji Moriyama
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    J Biol Chem 287:23977-94. 2012
    ..Thus, EBNA1 can direct localized assembly of Orc in a process that is facilitated by Cdc6. The possibility of similar modes of recruitment of Orc/Cdc6 at the human chromosomal origins will be discussed...
  34. pmc Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis
    Jung Min Kim
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
    EMBO J 22:5260-72. 2003
    ..Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals...
  35. doi request reprint Eukaryotic chromosome DNA replication: where, when, and how?
    Hisao Masai
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156 8506, Japan
    Annu Rev Biochem 79:89-130. 2010
    ..We review here various regulatory mechanisms that control the replication program in eukaryotes and discuss future directions in this dynamic field...
  36. doi request reprint Purification of replication factors using insect and mammalian cell expression systems
    Shuji Uno
    Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Methods 57:214-21. 2012
    ..Availability of efficient methods to overproduce and purify the proteins that have been challenging would facilitate the enzymatic analyses of the processes of DNA replication...
  37. pmc Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1
    Masako Oda
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    PLoS ONE 7:e42375. 2012
    ..It may be determined by Mb-domain structures, termed as "replication domains", and recent findings indicate that replication timing is under developmental and cell type-specific regulation...
  38. ncbi request reprint DNA binding of PriA protein requires cooperation of the N-terminal D-loop/arrested-fork binding and C-terminal helicase domains
    Taku Tanaka
    Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Biol Chem 277:38062-71. 2002
    ....
  39. doi request reprint Stalled replication forks: making ends meet for recognition and stabilization
    Hisao Masai
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Bioessays 32:687-97. 2010
    ..Elucidation of the structural basis for recognition of arrested forks by PriA should provide useful insight into how stalled forks are recognized in eukaryotes...
  40. doi request reprint Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells: DNA-binding activity and novel protein interactions
    Syuzi Uno
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo 156 8506, Japan
    Genes Cells 16:842-56. 2011
    ..We will also discuss the advantage of this system for purification and characterization of those proteins that are large and have been difficult to deal with...
  41. doi request reprint Cdc7 as a potential new target for cancer therapy
    Sayuri Ito
    Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo, Japan
    Drug News Perspect 21:481-8. 2008
    ..Thus, Cdc7 kinase may be a promising novel target for cancer therapy. Indeed, the first classes of Cdc7 inhibitors have been reported and have been shown to be effective in delaying tumor growth in animal models...
  42. ncbi request reprint Genetic dissection of mammalian Cdc7 kinase: cell cycle and developmental roles
    Jung Min Kim
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Cell Cycle 3:300-4. 2004
    ..These results from mammals will be discussed in conjunction with the pleiotropic effects of Cdc7 mutation observed in yeasts...
  43. ncbi request reprint A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase
    Masayuki Yamada
    Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
    J Biol Chem 277:27668-81. 2002
    ..Our results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site...
  44. pmc Inactivation of Cdc7 kinase in mouse ES cells results in S-phase arrest and p53-dependent cell death
    Jung Min Kim
    Department of Molecular and Developmental Biology, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, CREST, Tokyo 108 8639, Japan
    EMBO J 21:2168-79. 2002
    ....
  45. doi request reprint Replication timing regulation of eukaryotic replicons: Rif1 as a global regulator of replication timing
    Satoshi Yamazaki
    Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamkitazawa, Setagaya Ku, Tokyo 156 8506, Japan
    Trends Genet 29:449-60. 2013
    ..This review summarizes recent progress in the effort to elucidate the regulatory mechanisms of replication timing of eukaryotic replicons. ..
  46. pmc The Mini-Chromosome Maintenance (Mcm) Complexes Interact with DNA Polymerase α-Primase and Stimulate Its Ability to Synthesize RNA Primers
    Zhiying You
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    PLoS ONE 8:e72408. 2013
    ....
  47. doi request reprint RecQL4: a helicase linking formation and maintenance of a replication fork
    Hisao Masai
    Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo, Japan
    J Biochem 149:629-31. 2011
    ....
  48. pmc Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination
    Hiroyuki Sasanuma
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
    Genes Dev 22:398-410. 2008
    ..We thus propose that Cdc7, in concert with CDK, regulates Spo11 loading to DSB sites via Mer2 phosphorylation...
  49. ncbi request reprint Human origins of DNA replication selected from a library of nascent DNA
    Vesna Todorovic
    Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology, Padriciano, 99, 34012 Trieste, Italy
    Mol Cell 19:567-75. 2005
    ..This observation reinforces the universal paradigm that initiation of DNA replication takes place at, or in close proximity to, the binding sites of the trans-acting initiator proteins...
  50. ncbi request reprint Identification and characterization of a Xenopus homolog of Dbf4, a regulatory subunit of the Cdc7 protein kinase required for the initiation of DNA replication
    Asako Furukohri
    Research Institute for Microbial Diseases, and Graduate School of Science, Osaka University, Suita, Osaka 565 0871
    J Biochem 134:447-57. 2003
    ..These results suggest that the function of XDbf4-XCdc7 during the early embryonic cell cycle is regulated in a manner distinct from that during the somatic cell cycle...
  51. ncbi request reprint Functional interaction between tumor suppressor menin and activator of S-phase kinase
    Robert W Schnepp
    Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    Cancer Res 64:6791-6. 2004
    ..Together, these findings demonstrate a functional link between menin and ASK in the regulation of cell proliferation...
  52. ncbi request reprint Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest
    Megumi Kurita
    Department of Pharmacology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Biochem Biophys Res Commun 324:554-61. 2004
    ....
  53. pmc Structural basis of the 3'-end recognition of a leading strand in stalled replication forks by PriA
    Kaori Sasaki
    Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 26:2584-93. 2007
    ..This unique feature is prerequisite for the proper positioning of the helicase domain of PriA on the unreplicated double-stranded DNA...
  54. doi request reprint Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
    Asako Sakaue-Sawano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Cell 132:487-98. 2008
    ..These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events...
  55. pmc Nuclear import of Epstein-Barr virus nuclear antigen 1 mediated by NPI-1 (Importin alpha5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essential
    Ryo Kitamura
    AIDS Research Center, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku, Tokyo 162 8640, Japan
    J Virol 80:1979-91. 2006
    ....
  56. ncbi request reprint Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli
    Kaori Sasaki
    Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3 1 1, Fukuoka 812 8582, Japan
    Biochim Biophys Acta 1764:157-60. 2006
    ..We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA...
  57. ncbi request reprint A critical role of the 3' terminus of nascent DNA chains in recognition of stalled replication forks
    Toshimi Mizukoshi
    Department of Structural Biology, Biomolecular Engineering Research Institute, Suita, Osaka 565 0874, Japan
    J Biol Chem 278:42234-9. 2003
    ..The results suggest a mechanism by which stalled replication forks are recognized by a sensor protein for checkpoint responses...