Research Topics
Genomes and Genes
| Tomohiro KonoSummaryAffiliation: Tokyo University of Agriculture Country: Japan Publications
| Collaborators
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Detail Information
Publications
Influence of epigenetic changes during oocyte growth on nuclear reprogramming after nuclear transferT Kono
NODAI Research Institute, Tokyo University of Agriculture, Japan
Reprod Fertil Dev 10:593-8. 1998....- Nuclear competence for maturation and pronuclear formation in mouse oocytesSiqin Bao
Department of Animal Science, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan
Hum Reprod 17:1311-6. 2002..CONCLUSION: The factors required for pronuclear formation are derived from fully grown GV oocytes, and the transformation from decondensed sperm chromatin to a recondensed male pronucleus is governed by GV-derived factors... 
Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopediaPiero Carninci
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
Genome Res 13:1273-89. 2003..Clones accounting for about half of the predicted TUs await further sequencing. The continued high-discovery rate suggests that the task of transcriptome discovery is not yet complete...- Discovery of imprinted transcripts in the mouse transcriptome using large-scale expression profilingItoshi Nikaido
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku Yokohama, Kanagawa 230-0045, Japan
Genome Res 13:1402-9. 2003..These data provide a major resource for understanding the role of imprinting in mammalian inherited traits... - Oocyte growth-dependent progression of maternal imprinting in miceHitoshi Hiura
Department of Bioscience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
Genes Cells 11:353-61. 2006..From these results, we concluded that DNA methylation is established with oocyte size dependent manner, not with aging of mice... - Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestationTomohiro Kono
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
Dev Biol 243:294-300. 2002..The present results suggest that the cessation of H19 gene expression from the ng-allele causes extended development of the fetus and that functional defects in the placenta could be fatal for the ontogeny... - Birth of parthenogenetic mice that can develop to adulthoodTomohiro Kono
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
Nature 428:860-4. 2004..The parthenote developed to adulthood with the ability to reproduce offspring. These results suggest that paternal imprinting prevents parthenogenesis, ensuring that the paternal contribution is obligatory for the descendant... - [Prevention of parthenogenesis by genomic imprinting in mammals]Tomohiro Kono
Tanpakushitsu Kakusan Koso 49:2123-30. 2004 - Genomic imprinting is a barrier to parthenogenesis in mammalsT Kono
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
Cytogenet Genome Res 113:31-5. 2006..The resulting ng (non-growing)/fg (fully-grown) parthenogenic embryos were developed to term. Here, we discuss how the parthenogenetic embryos survived as normal individuals... - Genetic modification for bimaternal embryo developmentTomohiro Kono
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
Reprod Fertil Dev 21:31-6. 2009..Using bimaternal embryos with two sets of maternal genomes, the present paper illustrates how parental methylation imprints are an obstacle to the progression of parthenogenesis... - Defining contributions of paternally methylated imprinted genes at the Igf2-H19 and Dlk1-Gtl2 domains to mouse placentation by transcriptomic analysisManabu Kawahara
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
J Biol Chem 284:17751-65. 2009..These results provide a framework to further explore the role of epigenetic modifications in paternal genome during mouse placentation... - Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to termManabu Kawahara
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
FEBS Lett 581:5178-84. 2007..Foetuses derived from ng(Deltach12)/fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2+/- ng/fg genomes suggests that bi-allelic H19 expression might be involved in foetal development... 
Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in miceNozomi Takahashi
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
Hum Mol Genet 18:1879-88. 2009..Our results show that these phenotypes occur due to altered expression of the Dlk1-Dio3 cluster genes including miRNAs and snoRNAs via the cis and trans effects...- Regulated expression of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to termQiong Wu
Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Reproduction 131:481-8. 2006.... - Imprinted DNA methylation reprogramming during early mouse embryogenesis at the Gpr1-Zdbf2 locus is linked to long cis-intergenic transcriptionHisato Kobayashi
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
FEBS Lett 586:827-33. 2012..Globally, our findings suggest the role of Zdbf2linc in the establishment of secondary epigenetic modifications after implantation... - Complementary roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse placentationManabu Kawahara
Department of BioScience and Electron Microscope Centre, Tokyo University of Agriculture, Japan
Hum Mol Genet 15:2869-79. 2006..Our findings demonstrate that the genes controlled by the two paternally methylated regions, H19-DMR and IG-DMR, complementarily organize placentation... - Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marksHisato Kobayashi
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
PLoS Genet 8:e1002440. 2012..The present data provide entirely new perspectives on the evaluation of epigenetic markers in germline cells... - Synergistic role of Igf2 and Dlk1 in fetal liver development and hematopoiesis in bi-maternal miceQiong Wu
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
J Reprod Dev 54:177-82. 2008..These data suggest that inappropriate expression of Igf2 and Dlk1 is involved in impaired fetal hematopoiesis... - DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germlineHitoshi Hiura
Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Tokyo 156 8502, Japan
FEBS Lett 581:1255-60. 2007..The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline... - Epigenetically immature oocytes lead to loss of imprinting during embryogenesisYayoi Obata
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
J Reprod Dev 57:327-34. 2011..They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes... - Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2Hisato Kobayashi
Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
Genomics 93:461-72. 2009..Thus, our findings defined mouse chromosome 1 and human chromosome 2 as the loci for imprinted genes... - Disruption of parental-specific expression of imprinted genes in uniparental fetusesHidehiko Ogawa
Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan
FEBS Lett 580:5377-84. 2006..These results suggest that mechanisms acting in trans between paternal and maternal alleles are involved in the appropriate expression of some imprinted genes... - Nuclei of oocytes derived from mouse parthenogenetic embryos are competent to support development to termKatsutoshi Niwa
Department of Bioscience, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
Biol Reprod 71:1560-7. 2004..One developed normally to a fertile adult. These results revealed that oocytes derived from ng/fg PE can be normally imprinted during oogenesis and acquire competence to participate in development as female genomes... - Identification of genes aberrantly expressed in mouse embryonic stem cell-cloned blastocystsYuko Jincho
Department of Bioscience, Tokyo University of Agriculture, Tokyo 156 8502, Japan
Biol Reprod 78:568-76. 2008.... - Restoration of Dlk1 and Rtl1 is necessary but insufficient to rescue lethality in intergenic differentially methylated region (IG-DMR)-deficient miceNozomi Takahashi
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 0054, Japan
J Biol Chem 285:26121-5. 2010..Thus, we inferred that paternally expressed genes are necessary for perinatal survivability and that maternally expressed ncRNAs are involved in postnatal lethality... - Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocystsAtsushi Fukuda
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
PLoS ONE 5:e11274. 2010..Our findings provide a framework to further understand the reprogramming in SECNT embryos... - Long-term effects of in vitro growth of mouse oocytes on their maturation and developmentYayoi Obata
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
J Reprod Dev 53:1183-90. 2007..05). We concluded that long-term culture of oocytes did not affect their nuclear ability to develop to term; however, fetal growth was affected by the culture duration or culture conditions during the initial phase of follicular growth... - Protocol for the production of viable bimaternal mouse embryosManabu Kawahara
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
Nat Protoc 3:197-209. 2008..This oocyte reconstruction system, as described in this protocol, could provide valuable guidelines for exploring the potential endowments of gametes and for conferring novel properties to them... - Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetusesYusuke Sotomaru
Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243 0034, Japan
J Biol Chem 277:12474-8. 2002.... - High-frequency generation of viable mice from engineered bi-maternal embryosManabu Kawahara
Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
Nat Biotechnol 25:1045-50. 2007..The results provide conclusive evidence that imprinted genes regulated by these two paternally methylated imprinting-control regions are the only paternal barrier that prevents the normal development of bi-maternal mouse fetuses to term... - Irreversible barrier to the reprogramming of donor cells in cloning with mouse embryos and embryonic stem cellsYukiko Ono
Department of Bioscience, Tokyo University of Agriculture, Tokyo 156-8502, Japan
Biol Reprod 75:210-6. 2006.... - Development of bovine oocytes reconstructed with a nucleus from growing stage oocytes after fertilization in vitroSiqin Bao
Department of Animal Science, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-0054, Japan
Theriogenology 59:1231-9. 2003..These results show that bovine oocyte genomes are capable of supporting term development before the oocytes grow to their full size, which suggests that growing stage oocytes can be directly used as a source of maternal genomes... - Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturationYayoi Obata
Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
Proc Natl Acad Sci U S A 105:13918-23. 2008..We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development... - Disruption of primary imprinting during oocyte growth leads to the modified expression of imprinted genes during embryogenesisY Obata
NODAI Research Institute, Tokyo University of Agriculture, Tokyo, Japan
Development 125:1553-60. 1998..Thus, our results show that primary imprinting during oocyte growth exhibits a crucial effect on both the expression and repression of maternal alleles during embryogenesis... - Developmental ability of trophoblast stem cells in uniparental mouse embryosH Ogawa
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
Placenta 30:448-56. 2009..These results suggest that PG embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells... - Disruption of imprinting in cloned mouse fetuses from embryonic stem cellsH Ogawa
Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
Reproduction 126:549-57. 2003..These results show that inappropriate reprogramming in some imprinted genes affects the development of cloned embryos, and that aberrant P0 Igf2 transcription in particular may cause the overgrowth of cloned fetuses and placentae... - DNA methylation analysis of germ cells by using bisulfite-based sequencing methodsHisato Kobayashi
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
Methods Mol Biol 825:223-35. 2012..Here, we introduce two methods for bisulfite-based DNA methylation analyses using small samples such as germ cells: bisulfite Sanger sequencing at a specific locus and high-throughput bisulfite sequencing at the whole genome level... - Whole-genome resequencing shows numerous genes with nonsynonymous SNPs in the Japanese native cattle Kuchinoshima-UshiRyouka Kawahara-Miki
Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo, Japan
BMC Genomics 12:103. 2011.... - Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetusesY Sotomaru
Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi-shi, Kanagawa 243-0034, Japan
J Biol Chem 276:26694-8. 2001..This suggests that both parental genomes are necessary to establish parental specific expression of the U2afbp-rs gene... - Production of cloned mice from embryonic stem cells arrested at metaphaseY Ono
Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atsugi-shi, Kanagawa 243-0034, Japan
Reproduction 122:731-6. 2001..This study showed that cloning with embryonic stem cells is a viable procedure resulting in the production of transgenic cloned adults... - Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertilityMegumu Ito
Department of Perinatal Medicine and Maternal Care, Division of Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan
Fertil Steril 90:1026-35. 2008..The goal of this study was to identify a reliable biomarker for age-related infertility... - Gene silencing: maturation of mouse fetal germ cells in vitroYayoi Obata
Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan
Nature 418:497. 2002..To our knowledge, this is the first time that complete oogenesis has been successfully accomplished in vitro... - The human HYMAI/PLAGL1 differentially methylated region acts as an imprint control region in miceTakahiro Arima
Division of Molecular and Cell Therapeutics, Department of Molecular Genetics, Kyusyu University, Beppu, Oita 874 0838, Japan
Genomics 88:650-8. 2006..Therefore, we propose that this DMR functions as the IC for the HYMAI/PLAGL1 domain... - Histological study of the hypertrophic placentas and open eyelids observed in cloned fetusesNobuhiro Shimozawa
Central Institute for Experimental Animals, Kawasaki, Kanaga, Japan
J Reprod Dev 49:221-6. 2003.... - Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytesToshiaki Watanabe
Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
Nature 453:539-43. 2008..Our results reveal a role for endogenous siRNAs in mammalian oocytes and show that organisms lacking RdRP activity can produce functional endogenous siRNAs from naturally occurring dsRNAs... - Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarrayYosuke Mizuno
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230 0045, Japan
Biochem Biophys Res Commun 290:1499-505. 2002..The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray... - Zygotically activated genes are suppressed in mouse nuclear transferred embryosToru Suzuki
Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Cloning Stem Cells 8:295-304. 2006.... - Maternal primary imprinting is established at a specific time for each gene throughout oocyte growthYayoi Obata
Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan
J Biol Chem 277:5285-9. 2002..The developmental ability of the constructed parthenogenetic embryos was gradually reduced as the nuclear donor oocytes grew. These studies provide the first insight into the process of primary imprinting during oocyte growth... - Imprinting of a small nucleolar RNA gene on mouse chromosome 12Masafumi Shimoda
Gene Research Center, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan
Genomics 79:483-6. 2002.... - Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progenyNobuhiro Shimozawa
Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
Exp Anim 51:375-81. 2002.... - [Abnormalities in cloned mice are not transmitted to the progeny]Nobuhiro Shimozawa
Tanpakushitsu Kakusan Koso 47:1810-5. 2002 - Abnormalities in cloned mice are not transmitted to the progenyNobuhiro Shimozawa
Central Institute for Experimental Animals, Kanagawa, Japan
Genesis 34:203-7. 2002.... - Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cellsArata Honda
BioResource Center, RIKEN, Tsukuba, Ibaraki 305 0074, Japan
Proc Natl Acad Sci U S A 104:12389-94. 2007.... - Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transferTakahide Teranishi
Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
Dev Biol 266:76-86. 2004..These results suggest that rapid replacement of H1c with H1foo may play an important role in nuclear remodeling... - Phenotypic abnormalities observed in aged cloned mice from embryonic stem cells after long-term maintenanceNobuhiro Shimozawa
Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216-0001, Japan
Reproduction 132:435-41. 2006..These results indicate that the postnatal abnormalities observed in aged cloned mice are varied and can be restored through the germ line... - Pluripotential competence of cells associated with Nanog activityShin Ya Hatano
Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Mech Dev 122:67-79. 2005..Thus, the activity of Nanog is tightly correlated with an undifferentiated state of cells even in nuclear reprogrammed somatic cells. Nanog may function as a key regulator for sustaining pluripotency in a dose-dependent manner... - Gene therapy for progeny of mito-mice carrying pathogenic mtDNA by nuclear transplantationAkitsugu Sato
Graduate School of Life and Environmental Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Ibaraki 305-8572, Japan
Proc Natl Acad Sci U S A 102:16765-70. 2005..This procedure should be applicable to patients with mitochondrial diseases for preventing their children from developing the diseases...