Tomohiro Kono

Summary

Affiliation: Tokyo University of Agriculture
Country: Japan

Publications

  1. ncbi request reprint Influence of epigenetic changes during oocyte growth on nuclear reprogramming after nuclear transfer
    T Kono
    NODAI Research Institute, Tokyo University of Agriculture, Japan
    Reprod Fertil Dev 10:593-8. 1998
  2. ncbi request reprint Nuclear competence for maturation and pronuclear formation in mouse oocytes
    Siqin Bao
    Department of Animal Science, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    Hum Reprod 17:1311-6. 2002
  3. pmc Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia
    Piero Carninci
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1273-89. 2003
  4. pmc Discovery of imprinted transcripts in the mouse transcriptome using large-scale expression profiling
    Itoshi Nikaido
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1402-9. 2003
  5. ncbi request reprint Oocyte growth-dependent progression of maternal imprinting in mice
    Hitoshi Hiura
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Genes Cells 11:353-61. 2006
  6. pmc Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocysts
    Atsushi Fukuda
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    PLoS ONE 5:e11274. 2010
  7. pmc Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marks
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    PLoS Genet 8:e1002440. 2012
  8. pmc The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal–spatial-specific reprogramming
    Feng Cao
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 145:277-88. 2013
  9. pmc Epigenetic and transcriptional features of the novel human imprinted lncRNA GPR1AS suggest it is a functional ortholog to mouse Zdbf2linc
    Hisato Kobayashi
    Department of BioScience Tokyo University of Agriculture Tokyo, Japan
    Epigenetics 8:635-45. 2013
  10. ncbi request reprint [Prevention of parthenogenesis by genomic imprinting in mammals]
    Tomohiro Kono
    Tanpakushitsu Kakusan Koso 49:2123-30. 2004

Detail Information

Publications66

  1. ncbi request reprint Influence of epigenetic changes during oocyte growth on nuclear reprogramming after nuclear transfer
    T Kono
    NODAI Research Institute, Tokyo University of Agriculture, Japan
    Reprod Fertil Dev 10:593-8. 1998
    ....
  2. ncbi request reprint Nuclear competence for maturation and pronuclear formation in mouse oocytes
    Siqin Bao
    Department of Animal Science, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    Hum Reprod 17:1311-6. 2002
    ..The present study was carried out to clarify when during the growth period an oocyte nucleus acquires the ability to promote pronuclei formation after fertilization...
  3. pmc Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia
    Piero Carninci
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku, Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1273-89. 2003
    ..Clones accounting for about half of the predicted TUs await further sequencing. The continued high-discovery rate suggests that the task of transcriptome discovery is not yet complete...
  4. pmc Discovery of imprinted transcripts in the mouse transcriptome using large-scale expression profiling
    Itoshi Nikaido
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center GSC, RIKEN Yokohama Institute, Suehiro cho, Tsurumi ku Yokohama, Kanagawa 230 0045, Japan
    Genome Res 13:1402-9. 2003
    ..These data provide a major resource for understanding the role of imprinting in mammalian inherited traits...
  5. ncbi request reprint Oocyte growth-dependent progression of maternal imprinting in mice
    Hitoshi Hiura
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Genes Cells 11:353-61. 2006
    ..From these results, we concluded that DNA methylation is established with oocyte size dependent manner, not with aging of mice...
  6. pmc Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocysts
    Atsushi Fukuda
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    PLoS ONE 5:e11274. 2010
    ..Our findings provide a framework to further understand the reprogramming in SECNT embryos...
  7. pmc Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marks
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    PLoS Genet 8:e1002440. 2012
    ..The present data provide entirely new perspectives on the evaluation of epigenetic markers in germline cells...
  8. pmc The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal–spatial-specific reprogramming
    Feng Cao
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 145:277-88. 2013
    ..Thus, the present study revealed that the preimplantation development of SeCNT embryos appears normal; however, the progression of incorrect reprogramming is concealed throughout development...
  9. pmc Epigenetic and transcriptional features of the novel human imprinted lncRNA GPR1AS suggest it is a functional ortholog to mouse Zdbf2linc
    Hisato Kobayashi
    Department of BioScience Tokyo University of Agriculture Tokyo, Japan
    Epigenetics 8:635-45. 2013
    ..Our findings also suggest that lncRNAs with highly conserved epigenetic and transcriptional regulation across species arose by divergent evolution from a common ancestor, if they do not have identical exon structures. ..
  10. ncbi request reprint [Prevention of parthenogenesis by genomic imprinting in mammals]
    Tomohiro Kono
    Tanpakushitsu Kakusan Koso 49:2123-30. 2004
  11. ncbi request reprint Birth of parthenogenetic mice that can develop to adulthood
    Tomohiro Kono
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Nature 428:860-4. 2004
    ..The parthenote developed to adulthood with the ability to reproduce offspring. These results suggest that paternal imprinting prevents parthenogenesis, ensuring that the paternal contribution is obligatory for the descendant...
  12. ncbi request reprint Genetic modification for bimaternal embryo development
    Tomohiro Kono
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Reprod Fertil Dev 21:31-6. 2009
    ..Using bimaternal embryos with two sets of maternal genomes, the present paper illustrates how parental methylation imprints are an obstacle to the progression of parthenogenesis...
  13. ncbi request reprint Mouse parthenogenetic embryos with monoallelic H19 expression can develop to day 17.5 of gestation
    Tomohiro Kono
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Dev Biol 243:294-300. 2002
    ..The present results suggest that the cessation of H19 gene expression from the ng-allele causes extended development of the fetus and that functional defects in the placenta could be fatal for the ontogeny...
  14. ncbi request reprint Genomic imprinting is a barrier to parthenogenesis in mammals
    T Kono
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    Cytogenet Genome Res 113:31-5. 2006
    ..The resulting ng (non-growing)/fg (fully-grown) parthenogenic embryos were developed to term. Here, we discuss how the parthenogenetic embryos survived as normal individuals...
  15. pmc Defining contributions of paternally methylated imprinted genes at the Igf2-H19 and Dlk1-Gtl2 domains to mouse placentation by transcriptomic analysis
    Manabu Kawahara
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    J Biol Chem 284:17751-65. 2009
    ..These results provide a framework to further explore the role of epigenetic modifications in paternal genome during mouse placentation...
  16. doi request reprint Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Genomics 93:461-72. 2009
    ..Thus, our findings defined mouse chromosome 1 and human chromosome 2 as the loci for imprinted genes...
  17. ncbi request reprint Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term
    Manabu Kawahara
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    FEBS Lett 581:5178-84. 2007
    ..Foetuses derived from ng(Deltach12)/fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2+/- ng/fg genomes suggests that bi-allelic H19 expression might be involved in foetal development...
  18. ncbi request reprint Complementary roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse placentation
    Manabu Kawahara
    Department of BioScience and Electron Microscope Centre, Tokyo University of Agriculture, Japan
    Hum Mol Genet 15:2869-79. 2006
    ..Our findings demonstrate that the genes controlled by the two paternally methylated regions, H19-DMR and IG-DMR, complementarily organize placentation...
  19. doi request reprint Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice
    Nozomi Takahashi
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    Hum Mol Genet 18:1879-88. 2009
    ..Our results show that these phenotypes occur due to altered expression of the Dlk1-Dio3 cluster genes including miRNAs and snoRNAs via the cis and trans effects...
  20. ncbi request reprint Regulated expression of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to term
    Qiong Wu
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 131:481-8. 2006
    ....
  21. ncbi request reprint DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline
    Hitoshi Hiura
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Tokyo 156 8502, Japan
    FEBS Lett 581:1255-60. 2007
    ..The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline...
  22. doi request reprint Imprinted DNA methylation reprogramming during early mouse embryogenesis at the Gpr1-Zdbf2 locus is linked to long cis-intergenic transcription
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo, Japan
    FEBS Lett 586:827-33. 2012
    ..Globally, our findings suggest the role of Zdbf2linc in the establishment of secondary epigenetic modifications after implantation...
  23. ncbi request reprint Identification of genes aberrantly expressed in mouse embryonic stem cell-cloned blastocysts
    Yuko Jincho
    Department of Bioscience, Tokyo University of Agriculture, Tokyo 156 8502, Japan
    Biol Reprod 78:568-76. 2008
    ....
  24. ncbi request reprint Synergistic role of Igf2 and Dlk1 in fetal liver development and hematopoiesis in bi-maternal mice
    Qiong Wu
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    J Reprod Dev 54:177-82. 2008
    ..These data suggest that inappropriate expression of Igf2 and Dlk1 is involved in impaired fetal hematopoiesis...
  25. ncbi request reprint Epigenetically immature oocytes lead to loss of imprinting during embryogenesis
    Yayoi Obata
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    J Reprod Dev 57:327-34. 2011
    ..They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes...
  26. ncbi request reprint High-frequency generation of viable mice from engineered bi-maternal embryos
    Manabu Kawahara
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Nat Biotechnol 25:1045-50. 2007
    ..The results provide conclusive evidence that imprinted genes regulated by these two paternally methylated imprinting-control regions are the only paternal barrier that prevents the normal development of bi-maternal mouse fetuses to term...
  27. ncbi request reprint Nuclei of oocytes derived from mouse parthenogenetic embryos are competent to support development to term
    Katsutoshi Niwa
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Biol Reprod 71:1560-7. 2004
    ..One developed normally to a fertile adult. These results revealed that oocytes derived from ng/fg PE can be normally imprinted during oogenesis and acquire competence to participate in development as female genomes...
  28. ncbi request reprint Disruption of parental-specific expression of imprinted genes in uniparental fetuses
    Hidehiko Ogawa
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Tokyo 156 8502, Japan
    FEBS Lett 580:5377-84. 2006
    ..These results suggest that mechanisms acting in trans between paternal and maternal alleles are involved in the appropriate expression of some imprinted genes...
  29. doi request reprint Protocol for the production of viable bimaternal mouse embryos
    Manabu Kawahara
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Nat Protoc 3:197-209. 2008
    ..This oocyte reconstruction system, as described in this protocol, could provide valuable guidelines for exploring the potential endowments of gametes and for conferring novel properties to them...
  30. ncbi request reprint Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses
    Yusuke Sotomaru
    Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243 0034, Japan
    J Biol Chem 277:12474-8. 2002
    ....
  31. pmc Restoration of Dlk1 and Rtl1 is necessary but insufficient to rescue lethality in intergenic differentially methylated region (IG-DMR)-deficient mice
    Nozomi Takahashi
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 0054, Japan
    J Biol Chem 285:26121-5. 2010
    ..Thus, we inferred that paternally expressed genes are necessary for perinatal survivability and that maternally expressed ncRNAs are involved in postnatal lethality...
  32. ncbi request reprint Irreversible barrier to the reprogramming of donor cells in cloning with mouse embryos and embryonic stem cells
    Yukiko Ono
    Department of Bioscience, Tokyo University of Agriculture, Tokyo 156 8502, Japan
    Biol Reprod 75:210-6. 2006
    ....
  33. ncbi request reprint Long-term effects of in vitro growth of mouse oocytes on their maturation and development
    Yayoi Obata
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    J Reprod Dev 53:1183-90. 2007
    ..05). We concluded that long-term culture of oocytes did not affect their nuclear ability to develop to term; however, fetal growth was affected by the culture duration or culture conditions during the initial phase of follicular growth...
  34. doi request reprint Abundant sequence divergence in the native Japanese cattle Mishima-Ushi (Bos taurus) detected using whole-genome sequencing
    Kaoru Tsuda
    Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Genomics 102:372-8. 2013
    ..This study provides a framework for further genetic studies of Mishima-Ushi and research on the function of SNP-containing genes as well as understanding the genetic relationship between the domestic and native Japanese cattle breeds...
  35. pmc High-resolution DNA methylome analysis of primordial germ cells identifies gender-specific reprogramming in mice
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Genome Res 23:616-27. 2013
    ..Our data establish the basis for future studies on the role of epigenetic modifications in germline development and other biological processes...
  36. ncbi request reprint Development of bovine oocytes reconstructed with a nucleus from growing stage oocytes after fertilization in vitro
    Siqin Bao
    Department of Animal Science, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 0054, Japan
    Theriogenology 59:1231-9. 2003
    ..These results show that bovine oocyte genomes are capable of supporting term development before the oocytes grow to their full size, which suggests that growing stage oocytes can be directly used as a source of maternal genomes...
  37. pmc Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation
    Yayoi Obata
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Proc Natl Acad Sci U S A 105:13918-23. 2008
    ..We conclude that the cytoplasm of the XY oocyte is insufficient to support spindle formation at the second metaphase whereas its replacement with the cytoplasmic material from an XX oocyte allows normal development...
  38. ncbi request reprint Disruption of primary imprinting during oocyte growth leads to the modified expression of imprinted genes during embryogenesis
    Y Obata
    NODAI Research Institute, Tokyo University of Agriculture, Tokyo, Japan
    Development 125:1553-60. 1998
    ..Thus, our results show that primary imprinting during oocyte growth exhibits a crucial effect on both the expression and repression of maternal alleles during embryogenesis...
  39. ncbi request reprint Disruption of imprinting in cloned mouse fetuses from embryonic stem cells
    H Ogawa
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 126:549-57. 2003
    ..These results show that inappropriate reprogramming in some imprinted genes affects the development of cloned embryos, and that aberrant P0 Igf2 transcription in particular may cause the overgrowth of cloned fetuses and placentae...
  40. doi request reprint Developmental ability of trophoblast stem cells in uniparental mouse embryos
    H Ogawa
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    Placenta 30:448-56. 2009
    ..These results suggest that PG embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells...
  41. pmc Establishment of a Conditional Transgenic System Using the 2A Peptide in the Female Mouse Germline
    Satoshi Hara
    Department of Bioscience, Tokyo University of Agriculture, Tokyo 156 8502, Japan
    J Reprod Dev 60:250-5. 2014
    ..This novel transgenic mouse line based on the 2A expression system can serve as a useful tool for examining gene function during oogenesis. ..
  42. doi request reprint The transcriptomic architecture of mouse Sertoli cell clone embryos reveals temporal-spatial-specific reprogramming
    Feng Cao
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 145:277-88. 2013
    ..Thus, the present study revealed that the preimplantation development of SeCNT embryos appears normal; however, the progression of incorrect reprogramming is concealed throughout development...
  43. pmc Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting
    Satoshi Hara
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Hum Mol Genet 23:3853-64. 2014
    ....
  44. pmc Whole-genome resequencing shows numerous genes with nonsynonymous SNPs in the Japanese native cattle Kuchinoshima-Ushi
    Ryouka Kawahara-Miki
    Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo, Japan
    BMC Genomics 12:103. 2011
    ....
  45. doi request reprint DNA methylation analysis of germ cells by using bisulfite-based sequencing methods
    Hisato Kobayashi
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    Methods Mol Biol 825:223-35. 2012
    ..Here, we introduce two methods for bisulfite-based DNA methylation analyses using small samples such as germ cells: bisulfite Sanger sequencing at a specific locus and high-throughput bisulfite sequencing at the whole genome level...
  46. pmc Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia
    Yayoi Obata
    Department of Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Reproduction 147:H1-6. 2014
    ....
  47. doi request reprint Next-generation sequencing reveals genomic features in the Japanese quail
    Ryouka Kawahara-Miki
    Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1 1 1, Sakuragaoka, Setagaya Ku, Tokyo 156 8502, Japan
    Genomics 101:345-53. 2013
    ..The Japanese quail genome sequence obtained in the present study could enhance the value of this species as a model animal...
  48. ncbi request reprint Production of cloned mice from embryonic stem cells arrested at metaphase
    Y Ono
    Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atsugi-shi, Kanagawa 243-0034, Japan
    Reproduction 122:731-6. 2001
    ..This study showed that cloning with embryonic stem cells is a viable procedure resulting in the production of transgenic cloned adults...
  49. ncbi request reprint Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses
    Y Sotomaru
    Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi-shi, Kanagawa 243-0034, Japan
    J Biol Chem 276:26694-8. 2001
    ..This suggests that both parental genomes are necessary to establish parental specific expression of the U2afbp-rs gene...
  50. ncbi request reprint Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility
    Megumu Ito
    Department of Perinatal Medicine and Maternal Care, Division of Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan
    Fertil Steril 90:1026-35. 2008
    ..The goal of this study was to identify a reliable biomarker for age-related infertility...
  51. ncbi request reprint The human HYMAI/PLAGL1 differentially methylated region acts as an imprint control region in mice
    Takahiro Arima
    Division of Molecular and Cell Therapeutics, Department of Molecular Genetics, Kyusyu University, Beppu, Oita 874 0838, Japan
    Genomics 88:650-8. 2006
    ..Therefore, we propose that this DMR functions as the IC for the HYMAI/PLAGL1 domain...
  52. ncbi request reprint Gene silencing: maturation of mouse fetal germ cells in vitro
    Yayoi Obata
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Nature 418:497. 2002
    ..To our knowledge, this is the first time that complete oogenesis has been successfully accomplished in vitro...
  53. ncbi request reprint Maternal primary imprinting is established at a specific time for each gene throughout oocyte growth
    Yayoi Obata
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    J Biol Chem 277:5285-9. 2002
    ..The developmental ability of the constructed parthenogenetic embryos was gradually reduced as the nuclear donor oocytes grew. These studies provide the first insight into the process of primary imprinting during oocyte growth...
  54. doi request reprint Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 453:539-43. 2008
    ..Our results reveal a role for endogenous siRNAs in mammalian oocytes and show that organisms lacking RdRP activity can produce functional endogenous siRNAs from naturally occurring dsRNAs...
  55. ncbi request reprint Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray
    Yosuke Mizuno
    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230 0045, Japan
    Biochem Biophys Res Commun 290:1499-505. 2002
    ..The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray...
  56. ncbi request reprint Histological study of the hypertrophic placentas and open eyelids observed in cloned fetuses
    Nobuhiro Shimozawa
    Central Institute for Experimental Animals, Kawasaki, Kanaga, Japan
    J Reprod Dev 49:221-6. 2003
    ....
  57. ncbi request reprint Zygotically activated genes are suppressed in mouse nuclear transferred embryos
    Toru Suzuki
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Cloning Stem Cells 8:295-304. 2006
    ....
  58. pmc Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells
    Arata Honda
    BioResource Center, RIKEN, Tsukuba, Ibaraki 305 0074, Japan
    Proc Natl Acad Sci U S A 104:12389-94. 2007
    ....
  59. ncbi request reprint Phenotypic abnormalities observed in aged cloned mice from embryonic stem cells after long-term maintenance
    Nobuhiro Shimozawa
    Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
    Reproduction 132:435-41. 2006
    ..These results indicate that the postnatal abnormalities observed in aged cloned mice are varied and can be restored through the germ line...
  60. ncbi request reprint Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer
    Takahide Teranishi
    Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    Dev Biol 266:76-86. 2004
    ..These results suggest that rapid replacement of H1c with H1foo may play an important role in nuclear remodeling...
  61. ncbi request reprint Direct production of gene-targeted mice from ES cells by nuclear transfer and gene transmission to their progeny
    Nobuhiro Shimozawa
    Central Institute for Experimental Animals, 1430 Nogawa, Miyamae, Kawasaki, Kanagawa 216 0001, Japan
    Exp Anim 51:375-81. 2002
    ....
  62. pmc Gene therapy for progeny of mito-mice carrying pathogenic mtDNA by nuclear transplantation
    Akitsugu Sato
    Graduate School of Life and Environmental Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Ibaraki 305 8572, Japan
    Proc Natl Acad Sci U S A 102:16765-70. 2005
    ..This procedure should be applicable to patients with mitochondrial diseases for preventing their children from developing the diseases...
  63. ncbi request reprint Pluripotential competence of cells associated with Nanog activity
    Shin Ya Hatano
    Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Mech Dev 122:67-79. 2005
    ..Thus, the activity of Nanog is tightly correlated with an undifferentiated state of cells even in nuclear reprogrammed somatic cells. Nanog may function as a key regulator for sustaining pluripotency in a dose-dependent manner...
  64. ncbi request reprint Abnormalities in cloned mice are not transmitted to the progeny
    Nobuhiro Shimozawa
    Central Institute for Experimental Animals, Kanagawa, Japan
    Genesis 34:203-7. 2002
    ....
  65. ncbi request reprint Imprinting of a small nucleolar RNA gene on mouse chromosome 12
    Masafumi Shimoda
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma, 371 8511, Japan
    Genomics 79:483-6. 2002
    ....
  66. ncbi request reprint [Abnormalities in cloned mice are not transmitted to the progeny]
    Nobuhiro Shimozawa
    Tanpakushitsu Kakusan Koso 47:1810-5. 2002