Masayuki Komada

Summary

Affiliation: Tokyo Institute of Technology
Country: Japan

Publications

  1. ncbi request reprint Controlling receptor downregulation by ubiquitination and deubiquitination
    Masayuki Komada
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    Curr Drug Discov Technol 5:78-84. 2008
  2. pmc [Beta]IV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier
    Masayuki Komada
    Program in Developmental Biology and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Biol 156:337-48. 2002
  3. pmc STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif
    Emi Mizuno
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Mol Biol Cell 14:3675-89. 2003
  4. ncbi request reprint The Hrs/STAM complex in the downregulation of receptor tyrosine kinases
    Masayuki Komada
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    J Biochem 137:1-8. 2005
  5. ncbi request reprint A deubiquitinating enzyme UBPY regulates the level of protein ubiquitination on endosomes
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Traffic 7:1017-31. 2006
  6. pmc Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Mol Biol Cell 16:5163-74. 2005
  7. ncbi request reprint A role for Hrs in endosomal sorting of ligand-stimulated and unstimulated epidermal growth factor receptor
    Chitose Morino
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226 8501, Japan
    Exp Cell Res 297:380-91. 2004
  8. ncbi request reprint 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Yokohama, Japan
    Exp Cell Res 313:3624-34. 2007
  9. ncbi request reprint Association with Hrs is required for the early endosomal localization, stability, and function of STAM
    Emi Mizuno
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8501
    J Biochem 135:385-96. 2004
  10. doi request reprint Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis
    Akiko Mukai
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Yokohama 226 8501, Japan
    J Cell Sci 121:1325-33. 2008

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Controlling receptor downregulation by ubiquitination and deubiquitination
    Masayuki Komada
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    Curr Drug Discov Technol 5:78-84. 2008
    ..Therefore, elucidating the entire functions and regulation of the endosomal DUBs potentially provides novel molecular targets for the treatment of cancer accompanied by overexpression or constitutive activation of RTKs...
  2. pmc [Beta]IV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier
    Masayuki Komada
    Program in Developmental Biology and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Biol 156:337-48. 2002
    ..These results indicate that betaIV-spectrin and ankyrin-G mutually stabilize the membrane protein cluster and the linked membrane cytoskeleton at AIS and NR...
  3. pmc STAM proteins bind ubiquitinated proteins on the early endosome via the VHS domain and ubiquitin-interacting motif
    Emi Mizuno
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Mol Biol Cell 14:3675-89. 2003
    ....
  4. ncbi request reprint The Hrs/STAM complex in the downregulation of receptor tyrosine kinases
    Masayuki Komada
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    J Biochem 137:1-8. 2005
    ..Recent studies have shown that the Hrs/STAM complex binds ubiquitin moieties and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes...
  5. ncbi request reprint A deubiquitinating enzyme UBPY regulates the level of protein ubiquitination on endosomes
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Traffic 7:1017-31. 2006
    ..These results suggest that UBPY regulates the level of protein ubiquitination on endosomes, which is required for maintaining the morphology of the organelle...
  6. pmc Regulation of epidermal growth factor receptor down-regulation by UBPY-mediated deubiquitination at endosomes
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Mol Biol Cell 16:5163-74. 2005
    ..We conclude that UBPY negatively regulates the rate of EGFR down-regulation by deubiquitinating EGFR on endosomes...
  7. ncbi request reprint A role for Hrs in endosomal sorting of ligand-stimulated and unstimulated epidermal growth factor receptor
    Chitose Morino
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama 226 8501, Japan
    Exp Cell Res 297:380-91. 2004
    ....
  8. ncbi request reprint 14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase
    Emi Mizuno
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Yokohama, Japan
    Exp Cell Res 313:3624-34. 2007
    ..We conclude that UBPY is catalytically inhibited in a phosphorylation-dependent manner by 14-3-3s during the interphase, and this regulation is cancelled in the M phase...
  9. ncbi request reprint Association with Hrs is required for the early endosomal localization, stability, and function of STAM
    Emi Mizuno
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8501
    J Biochem 135:385-96. 2004
    ....
  10. doi request reprint Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis
    Akiko Mukai
    Department of Biological Sciences, Tokyo Institute of Technology, 4259 B 16 Nagatsuta, Yokohama 226 8501, Japan
    J Cell Sci 121:1325-33. 2008
    ..Our results thus implicate the ubiquitylation/deubiquitylation of proteins including VAMP8 in cytokinesis...
  11. pmc Nucleophosmin/B23 regulates ubiquitin dynamics in nucleoli by recruiting deubiquitylating enzyme USP36
    Akinori Endo
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 284:27918-23. 2009
    ..We conclude that nucleophosmin/B23 recruits USP36 to nucleoli, thereby serving as a platform for the regulation of nucleolar protein functions through ubiquitylation/deubiquitylation...
  12. pmc Ubiquitin-interacting motifs confer full catalytic activity, but not ubiquitin chain substrate specificity, to deubiquitinating enzyme USP37
    Hidetaka Tanno
    From the Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 289:2415-23. 2014
    ..These results suggested that the UIMs in USP37 contribute to the full enzymatic activity, but not ubiquitin chain substrate specificity, of USP37 possibly by holding the ubiquitin chain substrate in the proximity of the catalytic core. ..
  13. doi request reprint Ubiquitin-mediated proteasomal degradation of non-synonymous SNP variants of human ABC transporter ABCG2
    Hiroshi Nakagawa
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Biochem J 411:623-31. 2008
    ..Control of proteasomal degradation of ABCG2 would provide a novel approach in cancer chemotherapy to circumvent multidrug resistance of human cancers...
  14. doi request reprint Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36
    Akinori Endo
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Cell Sci 122:678-86. 2009
    ..We conclude that by deubiquitylating various nucleolar substrate proteins including nucleophosmin/B23 and fibrillarin, USP36 plays a crucial role in regulating the structure and function of nucleoli...
  15. ncbi request reprint Specific role of the truncated betaIV-spectrin Sigma6 in sodium channel clustering at axon initial segments and nodes of ranvier
    Yoko Uemoto
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 282:6548-55. 2007
    ..We conclude that the truncated betaIV-spectrin isoform Sigma6 plays a specific role in clustering voltage-gated sodium channels, whereas it is dispensable for membrane stabilization at axon initial segments and nodes of Ranvier...
  16. ncbi request reprint Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes
    Michihiko Nakamura
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Genes Cells 11:593-606. 2006
    ..In contrast, a mutant of AMSH that lacks the ability to bind STAM localized normally on endosomes. We suggest that AMSH and AMSH-LP are anchored on the early endosomal membrane via interaction with the clathrin coat...
  17. doi request reprint The ubiquitin code and its decoding machinery in the endocytic pathway
    Hidetaka Tanno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biochem 153:497-504. 2013
    ..In this review, we discuss the types of ubiquitination that drive these trafficking processes, and how the ubiquitin (Ub) modifications are recognized by specific Ub-binding proteins...
  18. pmc The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane
    Hidetaka Tanno
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan
    Mol Biol Cell 23:1343-53. 2012
    ..We conclude that by binding to the Lys-63-linked polyubiquitin moiety of EGFR at the plasma membrane, Ankrd 13 proteins regulate the rapid internalization of ligand-activated EGFR...
  19. pmc The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A
    Akinori Yamasaki
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    Mol Biol Cell 17:4876-87. 2006
    ..We conclude that ALG-2 is recruited to ER exit sites via Ca(2+)-dependent interaction with Sec31A and in turn stabilizes the localization of Sec31A at these sites...
  20. ncbi request reprint Intramolecular disulfide bond is a critical check point determining degradative fates of ATP-binding cassette (ABC) transporter ABCG2 protein
    Kanako Wakabayashi
    Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    J Biol Chem 282:27841-6. 2007
    ..Namely, the WT ABCG2 is degraded in lysosomes, and the misfolded ABCG2 lacking intramolecular disulfide bond undergoes ubiquitin-mediated protein degradation in proteasomes...
  21. ncbi request reprint ERK-dependent downregulation of Skp2 reduces Myc activity with HGF, leading to inhibition of cell proliferation through a decrease in Id1 expression
    Xiaoran Li
    Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama, 226 8501, Japan
    Mol Cancer Res 11:1437-47. 2013
    ..Implications: This study elucidates the molecular details of HGF-induced inhibition of cellular proliferation in liver cancer cells...
  22. pmc Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor
    Kimitoshi Denda
    Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226 8501, Japan
    J Biol Chem 286:28802-10. 2011
    ..We thus provide evidence for a novel labor-inducing fetoplacental signal that depends on the X chromosome and possibly arises from the placenta...
  23. ncbi request reprint Functional analysis of the relationship between the neurofibromatosis 2 tumor suppressor and its binding partner, hepatocyte growth factor-regulated tyrosine kinase substrate
    Chun Xiao Sun
    Department of Neurology, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Mol Genet 11:3167-78. 2002
    ..These results suggest that merlin growth suppression requires HRS expression and that the binding of merlin to HRS may facilitate its ability to function as a tumor suppressor...
  24. ncbi request reprint Expression of protein 4.1G in Schwann cells of the peripheral nervous system
    Nobuhiko Ohno
    Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo City, Yamanashi, Japan
    J Neurosci Res 84:568-77. 2006
    ..These data support the concept that 4.1G plays an important role in the membrane expansion and specialization that occurs during formation and maintenance of myelin internodes in the peripheral nervous system...
  25. ncbi request reprint betaIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment
    Kazunari Nishimura
    Laboratory for Neuronal Growth Mechanisms, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Mol Cell Neurosci 34:422-30. 2007
    ..Our results highlight the role of betaIV-spectrin and ankyrinG as critical components of a selective barrier against L1CAM...
  26. doi request reprint Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains
    Yusuke Sato
    Structural Biology Laboratory, Life Science Division, Synchrotron Radiation Research Organization and Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113 0032, Japan
    Nature 455:358-62. 2008
    ..This is the first reported structure of a DUB in complex with an isopeptide-linked ubiquitin chain, which reveals the mechanism for Lys 63-linkage-specific deubiquitination by AMSH family members...