Akihito Ishigami


Affiliation: Tokyo Metropolitan Institute of Gerontology
Country: Japan


  1. Takisawa S, Funakoshi T, Yatsu T, Nagata K, Aigaki T, Machida S, et al. Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance. Sci Rep. 2019;9:4702 pubmed publisher
    ..Thus, longer-term AsA deficiency is associated with muscle wasting, that this can be reversed by restoring AsA levels. ..
  2. Takahashi K, Yanai S, Takisawa S, Kono N, Arai H, Nishida Y, et al. Vitamin C and vitamin E double-deficiency increased neuroinflammation and impaired conditioned fear memory. Arch Biochem Biophys. 2019;663:120-128 pubmed publisher
    ..The findings of this study provide evidence that VC and VE deficiency led to impaired conditioned fear memory possibly caused by neuroinflammation in the brain. ..
  3. Kawashima S, Funakoshi T, Sato Y, Saito N, Ohsawa H, Kurita K, et al. Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage. Sci Rep. 2018;8:16199 pubmed publisher
    ..Moreover, the pre-AA treatment was more effective at preventing UVB-induced skin damage than the post-AA treatment. In summary, pre- and post-AA treatments of the epidermis prevent UVB-induced damage. ..
  4. Takahashi K, Takisawa S, Shimokado K, Kono N, Arai H, Ishigami A. Age-related changes of vitamin E: α-tocopherol levels in plasma and various tissues of mice and hepatic α-tocopherol transfer protein. Eur J Nutr. 2017;56:1317-1327 pubmed publisher
    ..This is the first report to investigate VE in terms of the α-tocopherol levels in plasma and various tissues of mice and hepatic α-TTP protein levels during aging. ..
  5. Koizumi M, Kondo Y, Isaka A, Ishigami A, Suzuki E. Vitamin C impacts anxiety-like behavior and stress-induced anorexia relative to social environment in SMP30/GNL knockout mice. Nutr Res. 2016;36:1379-1391 pubmed publisher
    ..It should be noted that anorexia is extremely serious for the females. We conclude that endogenous VC status is critical for determining vulnerability to anxiety and anorexia in a sex-specific manner. ..
  6. Amano A, Kondo Y, Noda Y, Ohta M, Kawanishi N, Machida S, et al. Abnormal lipid/lipoprotein metabolism and high plasma testosterone levels in male but not female aromatase-knockout mice. Arch Biochem Biophys. 2017;622:47-58 pubmed publisher
    ..These results strongly suggest that Cyp19 gene disruption, which induces a sexually dimorphic response and high plasma testosterone levels in male mice, also induces hepatic steatosis. ..
  7. Takahashi K, Yanai S, Shimokado K, Ishigami A. Coffee consumption in aged mice increases energy production and decreases hepatic mTOR levels. Nutrition. 2017;38:1-8 pubmed publisher
    ..Moreover, coffee consumption by the aged population had a positive effect on behavioral energy and lipid metabolism. ..
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    Ishigami A, Kuramoto M, Yamada M, Watanabe K, Senshu T. Molecular cloning of two novel types of peptidylarginine deiminase cDNAs from retinoic acid-treated culture of a newborn rat keratinocyte cell line. FEBS Lett. 1998;433:113-8 pubmed
    ..Recombinant proteins encoded by both PAD-R11 and PAD-R4 showed the enzyme activities. That of PAD-R11 showed a characteristic feature of the enzyme found in the epidermis. ..
  9. Ishigami A, Fujita T, Handa S, Shirasawa T, Koseki H, Kitamura T, et al. Senescence marker protein-30 knockout mouse liver is highly susceptible to tumor necrosis factor-alpha- and Fas-mediated apoptosis. Am J Pathol. 2002;161:1273-81 pubmed
    ..Collectively, these results demonstrate that SMP30 acts to protect cells from apoptosis. ..

More Information


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    Ishigami A, Ohsawa T, Hiratsuka M, Taguchi H, Kobayashi S, Saito Y, et al. Abnormal accumulation of citrullinated proteins catalyzed by peptidylarginine deiminase in hippocampal extracts from patients with Alzheimer's disease. J Neurosci Res. 2005;80:120-8 pubmed
  2. Takahashi K, Kishimoto Y, Konishi T, Fujita Y, Ito M, Shimokado K, et al. Ascorbic acid deficiency affects genes for oxidation-reduction and lipid metabolism in livers from SMP30/GNL knockout mice. Biochim Biophys Acta. 2014;1840:2289-98 pubmed publisher
    ..Results from using this unique model of AA deficiency, the SMP30/GNL-KO mouse, now provide new information about formerly unknown AA functions that will implement further study of AA in vivo. ..
  3. Sase T, Arito M, Onodera H, Omoteyama K, Kurokawa M, Kagami Y, et al. Hypoxia-induced production of peptidylarginine deiminases and citrullinated proteins in malignant glioma cells. Biochem Biophys Res Commun. 2017;482:50-56 pubmed publisher
    ..Our data may contribute to understanding of pathophysiology of malignant gliomas from the aspects of protein citrullination. ..
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    Ishigami A, Ohsawa T, Asaga H, Akiyama K, Kuramoto M, Maruyama N. Human peptidylarginine deiminase type II: molecular cloning, gene organization, and expression in human skin. Arch Biochem Biophys. 2002;407:25-31 pubmed
    ..In an immunohistochemical study of human skin, the type II enzyme was expressed by all the living epidermal layers, suggesting that PAD type II is functionally important during terminal differentiation of epidermal keratinocytes. ..
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    Ishigami A, Fujita T, Inoue H, Handa S, Kubo S, Kondo Y, et al. Senescence marker protein-30 (SMP30) induces formation of microvilli and bile canaliculi in Hep G2 cells. Cell Tissue Res. 2005;320:243-9 pubmed
  6. Jang B, Ishigami A, Kim Y, Choi E. The Peptidylarginine Deiminase Inhibitor Cl-Amidine Suppresses Inducible Nitric Oxide Synthase Expression in Dendritic Cells. Int J Mol Sci. 2017;18: pubmed publisher
    ..Our findings indicate that Cl-amidine acts as a novel suppressor of iNOS expression, suggesting that Cl-amidine has the potential to ameliorate the effects of excessive iNOS/NO-linked immune responses. ..