Shin Ichi Hisanaga

Summary

Affiliation: Tokyo Metropolitan University
Country: Japan

Publications

  1. pmc Actin interaction and regulation of cyclin-dependent kinase 5/p35 complex activity
    Jiqing Xu
    Center for Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska, USA
    J Neurochem 116:192-204. 2011
  2. ncbi request reprint Brain-derived neurotrophic factor-induced phosphorylation of neurofilament-H subunit in primary cultures of embryo rat cortical neurons
    H Tokuoka
    Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    J Cell Sci 113:1059-68. 2000
  3. ncbi request reprint The regulation of cyclin-dependent kinase 5 activity through the metabolism of p35 or p39 Cdk5 activator
    Shin Ichi Hisanaga
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Tokyo, Japan
    Neurosignals 12:221-9. 2003
  4. pmc Isomerase Pin1 stimulates dephosphorylation of tau protein at cyclin-dependent kinase (Cdk5)-dependent Alzheimer phosphorylation sites
    Taeko Kimura
    Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    J Biol Chem 288:7968-77. 2013
  5. doi request reprint Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons
    Takahiro Fuchigami
    Department of Biological Sciences, Tokyo Metropolitan University, Minami Osawa, Hachioji, Tokyo, 192 0397, Japan
    Genes Cells 18:410-24. 2013
  6. ncbi request reprint Enhanced activation of Ca2+/calmodulin-dependent protein kinase II upon downregulation of cyclin-dependent kinase 5-p35
    Tomohisa Hosokawa
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Tokyo, Japan
    J Neurosci Res 84:747-54. 2006
  7. ncbi request reprint In vivo and in vitro phosphorylation at Ser-493 in the glutamate (E)-segment of neurofilament-H subunit by glycogen synthase kinase 3beta
    Takahiro Sasaki
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Ohsawa, Hachiohji, Tokyo 192 0397, Japan
    J Biol Chem 277:36032-9. 2002
  8. ncbi request reprint Developmental regulation of the proteolysis of the p35 cyclin-dependent kinase 5 activator by phosphorylation
    Taro Saito
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Osawa, Hachiohji, Tokyo 192 0397, Japan
    J Neurosci 23:1189-97. 2003
  9. doi request reprint LMTK1/AATYK1 is a novel regulator of axonal outgrowth that acts via Rab11 in a Cdk5-dependent manner
    Tetsuya Takano
    Department of Biological Sciences, Tokyo Metropolitan University, Tokyo 192 0397, Japan
    J Neurosci 32:6587-99. 2012
  10. ncbi request reprint Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation
    Hirotsugu Kamei
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1 1 Minami Osawa, Hachiohji, Tokyo 192 0397, Japan
    J Biol Chem 282:1687-94. 2007

Detail Information

Publications65

  1. pmc Actin interaction and regulation of cyclin-dependent kinase 5/p35 complex activity
    Jiqing Xu
    Center for Neurodegenerative Disorders, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska, USA
    J Neurochem 116:192-204. 2011
    ..Together, these results suggest a novel mechanism of actin cytoskeletal regulation of Cdk5/p35 activity...
  2. ncbi request reprint Brain-derived neurotrophic factor-induced phosphorylation of neurofilament-H subunit in primary cultures of embryo rat cortical neurons
    H Tokuoka
    Laboratory of Cell and Developmental Biology, Faculty of Biosciences, Tokyo Institute of Technology, Nagatsuta, Midori ku, Yokohama 226 8501, Japan
    J Cell Sci 113:1059-68. 2000
    ..These results suggest a possibility that synapse formation induces NF-H phosphorylation, at least in part, through activation of CDK5 by BDNF...
  3. ncbi request reprint The regulation of cyclin-dependent kinase 5 activity through the metabolism of p35 or p39 Cdk5 activator
    Shin Ichi Hisanaga
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Tokyo, Japan
    Neurosignals 12:221-9. 2003
    ..p39 is also a short-lived protein and cleaved to the N-terminal truncation form of p29 by calpain. How the metabolism of p39 is regulated, however, is a future problem to be investigated...
  4. pmc Isomerase Pin1 stimulates dephosphorylation of tau protein at cyclin-dependent kinase (Cdk5)-dependent Alzheimer phosphorylation sites
    Taeko Kimura
    Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    J Biol Chem 288:7968-77. 2013
    ..Together, these results indicate that Pin1 is generally involved in the regulation of Tau hyperphosphorylation and hence the etiology of tauopathies...
  5. doi request reprint Dab1-mediated colocalization of multi-adaptor protein CIN85 with Reelin receptors, ApoER2 and VLDLR, in neurons
    Takahiro Fuchigami
    Department of Biological Sciences, Tokyo Metropolitan University, Minami Osawa, Hachioji, Tokyo, 192 0397, Japan
    Genes Cells 18:410-24. 2013
    ..In addition, Tyr phosphorylation of Dab1 strengthened the binding to CIN85. These results suggest that CIN85 participates in Reelin signaling through the binding to Dab1...
  6. ncbi request reprint Enhanced activation of Ca2+/calmodulin-dependent protein kinase II upon downregulation of cyclin-dependent kinase 5-p35
    Tomohisa Hosokawa
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Tokyo, Japan
    J Neurosci Res 84:747-54. 2006
    ..These results suggest that Cdk5 activity suppresses CaMKII activation, and that the downregulation of Cdk5 activity after treatment withNMDA facilitates CaMKII activation, leading to the easier induction of long-term potentiation...
  7. ncbi request reprint In vivo and in vitro phosphorylation at Ser-493 in the glutamate (E)-segment of neurofilament-H subunit by glycogen synthase kinase 3beta
    Takahiro Sasaki
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Ohsawa, Hachiohji, Tokyo 192 0397, Japan
    J Biol Chem 277:36032-9. 2002
    ..GSK3beta in the spinal cord extract was associated with NF cytoskeletons. Taken together, we concluded that Ser-493 in the E-segment of NF-H is phosphorylated by GSK3beta in rat spinal cords...
  8. ncbi request reprint Developmental regulation of the proteolysis of the p35 cyclin-dependent kinase 5 activator by phosphorylation
    Taro Saito
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Osawa, Hachiohji, Tokyo 192 0397, Japan
    J Neurosci 23:1189-97. 2003
    ..These results suggest that the phosphorylation of p35 serves as a protective mechanism that suppresses the generation of p25 in developing brains...
  9. doi request reprint LMTK1/AATYK1 is a novel regulator of axonal outgrowth that acts via Rab11 in a Cdk5-dependent manner
    Tetsuya Takano
    Department of Biological Sciences, Tokyo Metropolitan University, Tokyo 192 0397, Japan
    J Neurosci 32:6587-99. 2012
    ..Thus, LMTK1 can negatively control axonal outgrowth by regulating Rab11A activity in a Cdk5-dependent manner, and Cdk5-LMTK1-Rab11 is a novel signaling pathway involved in axonal outgrowth...
  10. ncbi request reprint Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation
    Hirotsugu Kamei
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1 1 Minami Osawa, Hachiohji, Tokyo 192 0397, Japan
    J Biol Chem 282:1687-94. 2007
    ....
  11. pmc Quantitative measurement of in vivo phosphorylation states of Cdk5 activator p35 by Phos-tag SDS-PAGE
    Tomohisa Hosokawa
    Department of Biological Sciences, Graduate School of Science and Technology, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    Mol Cell Proteomics 9:1133-43. 2010
    ..These are the first quantitative and site-specific measurements of phosphorylation of p35, demonstrating the usefulness of Phos-tag SDS-PAGE for analysis of phosphorylation states of in vivo proteins...
  12. ncbi request reprint Phosphorylation of FTDP-17 mutant tau by cyclin-dependent kinase 5 complexed with p35, p25, or p39
    Fumika Sakaue
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Tokyo 192 039, Japan
    J Biol Chem 280:31522-9. 2005
    ....
  13. doi request reprint Regulation of mitochondrial transport and inter-microtubule spacing by tau phosphorylation at the sites hyperphosphorylated in Alzheimer's disease
    Kourosh Shahpasand
    Laboratory of Molecular Neuroscience, Tokyo Metropolitan University, Minami Osawa, Hachioji, Tokyo 192 0397, Japan
    J Neurosci 32:2430-41. 2012
    ..Hyperphosphorylation of the AT8 sites may contribute to axonal degeneration by disrupting mitochondrial transport in Alzheimer's disease...
  14. pmc Phosphorylation of AATYK1 by Cdk5 suppresses its tyrosine phosphorylation
    Koji Tsutsumi
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    PLoS ONE 5:e10260. 2010
    ..These results suggest a possibility that AATYK1A plays a role in early to recycling endosomes and its function is regulated by phosphorylation with Cdk5 or Src-family kinases...
  15. doi request reprint Membrane association facilitates degradation and cleavage of the cyclin-dependent kinase 5 activators p35 and p39
    Seiji Minegishi
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Osawa 1 1, Hachioji, Tokyo 192 0397, Japan
    Biochemistry 49:5482-93. 2010
    ..However, p35 and p39 show differences in degradation and cleavage rates, which may in fact underlie the distinct physiological and pathological functions of these two Cdk5 activators...
  16. ncbi request reprint Phosphorylation of p35 and p39 by Cdk5 determines the subcellular location of the holokinase in a phosphorylation-site-specific manner
    Akiko Asada
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    J Cell Sci 125:3421-9. 2012
    ..These results suggest that the subcellular localization of the Cdk5-activator complexes is determined by its kinase activity, and also implicate a role for p39-Cdk5 in the nucleus...
  17. ncbi request reprint Cdk5--p39 is a labile complex with the similar substrate specificity to Cdk5--p35
    Mari Yamada
    Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Minami Osawa, Hachiohji, Tokyo, Japan
    J Neurochem 102:1477-87. 2007
    ..The different stability between Cdk5--p35 and Cdk5--p39 suggests their distinct roles under different regulation mechanisms in neurons...
  18. ncbi request reprint Phosphorylation of adult type Sept5 (CDCrel-1) by cyclin-dependent kinase 5 inhibits interaction with syntaxin-1
    Makoto Taniguchi
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    J Biol Chem 282:7869-76. 2007
    ..These results indicate that the interaction of Sept5 with syntaxin-1 is regulated by the phosphorylation of Sept5_v1 at Ser17 by Cdk5-p35...
  19. doi request reprint Neuronal expression of two isoforms of mouse Septin 5
    Akiko Asada
    Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan
    J Neurosci Res 88:1309-16. 2010
    ..Thus, these different expression and phosphorylation patterns suggest isoform-specific functions for Sept5 and that a phosphorylation-specific antibody will be useful to study this idea...
  20. doi request reprint Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25
    Ko Sato
    Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    J Neurochem 117:504-15. 2011
    ..Therefore, impairment of CAST expression and its associated mechanisms may contribute to the pathogenesis of neurodegenerative disorders...
  21. doi request reprint Suppression of mutant Huntingtin aggregate formation by Cdk5/p35 through the effect on microtubule stability
    Sayuko Kaminosono
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    J Neurosci 28:8747-55. 2008
    ..This Cdk5 inhibition of htt aggregates is a novel mechanism different from htt phosphorylation and interaction with Cdk5 reported previously (Luo et al., 2005; Anne et al., 2007)...
  22. ncbi request reprint Activation of latent cyclin-dependent kinase 5 (Cdk5)-p35 complexes by membrane dissociation
    Ying Shan Zhu
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1 1 Minami Osawa, Hachiohji, Tokyo, Japan
    J Neurochem 94:1535-45. 2005
    ..Solubilization with detergent or high-salt solution activates Cdk5-p35 several fold, and this activation is reversible. Therefore, membrane interactions represent a novel mechanism for the regulation of Cdk5-p35 kinase activity...
  23. ncbi request reprint p25/cyclin-dependent kinase 5 promotes the progression of cell death in nucleus of endoplasmic reticulum-stressed neurons
    Taro Saito
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Tokyo, Japan
    J Neurochem 102:133-40. 2007
    ..Cdk5 inhibitors or dominant-negative Cdk5 suppressed ER stress-induced neuronal cell death. These findings indicate that p25/Cdk5 is a proapoptotic factor that promotes ER stress-induced neuronal cell death in nuclei...
  24. ncbi request reprint Different protofilament-dependence of the microtubule binding between MAP2 and MAP4
    Aya Kawachi
    Division of Biological Sciences, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Biochem Biophys Res Commun 305:72-8. 2003
    ..Weakened binding on Zinc-sheets was also observed in the projection domain-deletion mutants of MAP4, so that the difference in the protofilament-dependence would lie in the relatively conserved microtubule-binding domain...
  25. ncbi request reprint Control of cyclin-dependent kinase 5 (Cdk5) activity by glutamatergic regulation of p35 stability
    Fan Yan Wei
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Osawa, Hachiohji, Tokyo, Japan
    J Neurochem 93:502-12. 2005
    ..p35(-/-) mice exhibited a lower threshold for induction of long-term potentiation. Thus excitatory glutamatergic neurotransmission regulates Cdk5 activity through p35 degradation, and this pathway may contribute to plasticity...
  26. ncbi request reprint Apoptosis-associated tyrosine kinase is a Cdk5 activator p35 binding protein
    Naoyuki Honma
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Tokyo 192 0397, Japan
    Biochem Biophys Res Commun 310:398-404. 2003
    ..Both hAATYKs and KIAA0641 bound to and were phosphorylated by Cdk5/p35. These results suggest that both isoforms of hAATYK are novel Cdk5/p35-binding and substrate proteins...
  27. ncbi request reprint The projection domain of MAP4 suppresses the microtubule-bundling activity of the microtubule-binding domain
    Junko Iida
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1 1 Minami Ohsawa, Hachiohji, Tokyo 192 0397, Japan
    J Mol Biol 320:97-106. 2002
    ..The suppressive activity of the PJ domain was correlated with the length, but not the amino acid sequence, of the PJ...
  28. ncbi request reprint Regulation of the interaction of Disabled-1 with CIN85 by phosphorylation with Cyclin-dependent kinase 5
    Yutaka Sato
    Department of Biological Science, Tokyo Metropolitan University, Minami Osawa, Hachioji, Tokyo 192 0397, Japan
    Genes Cells 12:1315-27. 2007
    ..Together with previous results that CIN85 regulates actin assembly, present results raise the possibility that Cdk5 modulates actin dynamics through regulation of CIN85-Dab1 interaction by the Dab1 phosphorylation...
  29. doi request reprint AATYK1A phosphorylation by Cdk5 regulates the recycling endosome pathway
    Tetsuya Takano
    Department of Biological Sciences, Tokyo Metropolitan University, Minami Osawa, Hachioji, Japan
    Genes Cells 15:783-97. 2010
    ..They furthermore indicate that Cdk5 can disrupt ERC formation via Ser34 phosphorylation of AATYK1A. Finally, our data suggest a mechanism by which AATYK1A signaling couples Cdk5 to Rab11A activity...
  30. doi request reprint Myristoylation of p39 and p35 is a determinant of cytoplasmic or nuclear localization of active cyclin-dependent kinase 5 complexes
    Akiko Asada
    Department of Biological Sciences, Tokyo Metropolitan University, Hachiohji, Tokyo, Japan
    J Neurochem 106:1325-36. 2008
    ..The differential nuclear accumulation of p39 and p35 suggests their segregated functions, p35-Cdk5 in the cytoplasm and p39-Cdk5 in the nucleus...
  31. ncbi request reprint Regulation of membrane association and kinase activity of Cdk5-p35 by phosphorylation of p35
    Ko Sato
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Tokyo, Japan
    J Neurosci Res 85:3071-8. 2007
    ....
  32. pmc Phosphorylation of drebrin by cyclin-dependent kinase 5 and its role in neuronal migration
    Kazuya Tanabe
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    PLoS ONE 9:e92291. 2014
    ..These results suggest that Cdk5-p35 regulates neuronal migration through phosphorylation of drebrin in growth cone processes. ..
  33. ncbi request reprint 14-3-3 Mediates phosphorylation-dependent inhibition of the interaction between the ubiquitin E3 ligase Nedd4-2 and epithelial Na+ channels
    Kazunori Nagaki
    Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Tokyo 192 0397, Japan
    Biochemistry 45:6733-40. 2006
    ..et al. (2006) EMBO J. 25, 211-221], suggest that 14-3-3 suppresses ubiquitin E3 ligase activities by inhibiting the formation of the enzyme/substrate complex...
  34. pmc LMTK1 regulates dendritic formation by regulating movement of Rab11A-positive endosomes
    Tetsuya Takano
    Department of Biological Sciences, Tokyo Metropolitan University, Tokyo 192 0397, Japan
    Mol Biol Cell 25:1755-68. 2014
    ....
  35. doi request reprint Cyclin-dependent kinase 5 phosphorylates and induces the degradation of ataxin-2
    Akiko Asada
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan Electronic address
    Neurosci Lett 563:112-7. 2014
    ..These results suggest that Cdk5 controls the abundance of both normal and polyQ-expanded ataxin-2 protein in neurons, which implies that Cdk5 activity is a therapeutic approach for SCA2. ..
  36. ncbi request reprint Cysteine misincorporation in bacterially expressed human alpha-synuclein
    Masami Masuda
    Department of Molecular Neurobiology, Tokyo Institute of Psychiatry, 2 1 8 Kamikitazawa, Setagaya ku 156 8585, Tokyo, Japan
    FEBS Lett 580:1775-9. 2006
    ..To avoid potential artefacts, we recommend use of the Y136-TAT construct for the expression of human alpha-syn...
  37. ncbi request reprint Translocation of lysosomal cathepsin D caused by oxidative stress or proteasome inhibition in primary cultured neurons and astrocytes
    Yuri Miura
    Research Team for Functional Genomics, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan
    Biol Pharm Bull 33:22-8. 2010
    ..The possible mechanism of age-related accumulation of cathepsin D in the cytosol of the normal rat brain will be discussed...
  38. doi request reprint Regulation and role of cyclin-dependent kinase activity in neuronal survival and death
    Shin Ichi Hisanaga
    Molecular Neuroscience, Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    J Neurochem 115:1309-21. 2010
    ..Appropriate activity, localization, and regulation of Cdk5 may be critical for long-term survival of neurons, which is more than 80 years in the case of humans...
  39. pmc Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    PLoS ONE 5:e15553. 2010
    ..Therefore, to determine the role of CDKAL1 in insulin release from β cells, we studied insulin release profiles in CDKAL1 gene knockout (CDKAL1 KO) mice...
  40. pmc Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development
    Jun Hamazaki
    Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    Mol Cell Biol 27:6629-38. 2007
    ..Our results demonstrate that Rpn10-mediated degradation of ubiquitinated proteins, catalyzed by UIMs, is indispensable for mammalian life...
  41. doi request reprint Small molecule inhibitor of type I transforming growth factor-β receptor kinase ameliorates the inhibitory milieu in injured brain and promotes regeneration of nigrostriatal dopaminergic axons
    Nozomu Yoshioka
    Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
    J Neurosci Res 89:381-93. 2011
    ..These results indicate that inhibition of TGF-β signaling suppresses formation of the fibrotic scar and creates a permissive environment for axonal regeneration...
  42. pmc Effect of Pin1 or microtubule binding on dephosphorylation of FTDP-17 mutant Tau
    Kensuke Yotsumoto
    Department of Biological Sciences, Faculty of Science and Engineering, Tokyo Metropolitan University, 1 1 Minami Osawa, Hachioji, Tokyo 192 0397, Japan
    J Biol Chem 284:16840-7. 2009
    ..These results demonstrate that mutation of Tau and its association with microtubules may change the conformation of Tau, thereby suppressing dephosphorylation and potentially contributing to the etiology of tauopathies...
  43. doi request reprint Novel axonal distribution of neurofilament-H phosphorylated at the glycogen synthase kinase 3beta-phosphorylation site in its E-segment
    Takahiro Sasaki
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami Ohsawa, Hachiohji, Tokyo, Japan
    J Neurosci Res 87:3088-97. 2009
    ..These results indicate that Ser493 in the NF-H E-segment is a novel site that is phosphorylated in both the myelinated and the unmyelinated regions of axons...
  44. pmc Commitment of 1-methyl-4-phenylpyrinidinium ion-induced neuronal cell death by proteasome-mediated degradation of p35 cyclin-dependent kinase 5 activator
    Ryo Endo
    Laboratory of Molecular Neuroscience, Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    J Biol Chem 284:26029-39. 2009
    ..We propose a role for Cdk5-p35 as a survival factor in countering MPP+-induced neuronal cell death...
  45. ncbi request reprint Valproic acid downregulates Cdk5 activity via the transcription of the p35 mRNA
    Miyuki Takahashi
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan
    Biochem Biophys Res Commun 447:678-82. 2014
    ..The chronic administration of VPA also downregulated p35 in mouse brains. These results indicate that VPA regulates Cdk5 activity in neurons via p35 transcription mediated by HDAC inhibition. ..
  46. ncbi request reprint Truncation of the projection domain of MAP4 (microtubule-associated protein 4) leads to attenuation of microtubule dynamic instability
    Sofy Permana
    Division of Biological Sciences, Graduate School of Science, Nagoya University, Japan
    Cell Struct Funct 29:147-57. 2005
    ..Perhaps, this unusual profile could be due to the uneven distribution of PJ2 along the MT lattice. These results indicate that the PJ domain of MAP4 participates in the regulation of the dynamic instability...
  47. ncbi request reprint Morphological and biochemical changes of neurofilaments in aged rat sciatic nerve axons
    Atsuko Uchida
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachiohji, Japan
    J Neurochem 88:735-45. 2004
    ..Although the relationship between NF packing and reduced NF-M is not clear at present, these changes in NFs may be associated with age-dependent axonal degeneration diseases...
  48. doi request reprint Palmitoylation-dependent endosomal localization of AATYK1A and its interaction with Src
    Koji Tsutsumi
    Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan
    Genes Cells 13:949-64. 2008
    ..These results indicate that palmitoylation is a critical factor not only for the subcellular localization of AATYK1A but also for its interaction with Src...
  49. pmc Neuropathological similarities and differences between schizophrenia and bipolar disorder: a flow cytometric postmortem brain study
    Yoshitaka Hayashi
    Affective Disorders Research Team, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
    PLoS ONE 7:e33019. 2012
    ....
  50. ncbi request reprint Cdk5-induced neuronal cell death: The activation of the conventional Rb-E2F G 1 pathway in post-mitotic neurons
    Shin Ichi Hisanaga
    Tokyo Metropolitan University, Hachioji, Japan
    Cell Cycle 11:2049. 2012
    ..Comment on: Futatsugi A, et al. Cell Cycle 2012; 1603-10...
  51. ncbi request reprint Impairment of hippocampal long-term depression and defective spatial learning and memory in p35 mice
    Toshio Ohshima
    Laboratory for Developmental Neurobiology, Brain Science Institute, Hirosawa, Wako City, Saitama, Japan
    J Neurochem 94:917-25. 2005
    ..These results demonstrate that p35-dependent Cdk5 activity is important to learning and synaptic plasticity. Deletion of p35 may shift the substrate specificity of Cdk5 due to compensatory expression of p39...
  52. ncbi request reprint Identifying novel substrates for mouse Cdk5 kinase using the yeast Saccharomyces cerevisiae
    Youko Horiuchi
    Department of Microbiology and Immunology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    Genes Cells 11:1393-404. 2006
    ..Further screening with these systems will enable us to identify more novel substrates and regulators of Cdk5/p35, which will lead to the exploration of Cdk5 function in diverse cellular systems...
  53. ncbi request reprint [Structure and function of microtubule-associated proteins]
    Susumu Kotani
    Tanpakushitsu Kakusan Koso 51:535-42. 2006
  54. ncbi request reprint Tau phosphorylation by cyclin-dependent kinase 5/p39 during brain development reduces its affinity for microtubules
    Satoru Takahashi
    Functional Genomics Unit, NIDCR, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:10506-15. 2003
    ..Our data suggest that tau phosphorylation by Cdk5 may provide the neuronal microtubules with dynamic properties in a region-specific and developmentally regulated manner...
  55. pmc Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles
    Kazuhito Tomizawa
    Department of Physiology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    J Cell Biol 163:813-24. 2003
    ..Moreover, the phosphorylation of both proteins completely disrupted the copolymerization into a ring formation. Finally, phosphorylation of both proteins was undetectable in p35-deficient mice...
  56. ncbi request reprint Aggregate formation and phosphorylation of neurofilament-L Pro22 Charcot-Marie-Tooth disease mutants
    Takahiro Sasaki
    Nathan Kline Institute, New York University School of Medicine, Orangeburg, NY 10962, USA
    Hum Mol Genet 15:943-52. 2006
    ....
  57. ncbi request reprint Intragenic modifiers of hereditary spastic paraplegia due to spastin gene mutations
    Ingrid K Svenson
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Neurogenetics 5:157-64. 2004
    ..Our identification of S44L and P45Q as modifiers of the HSP phenotype suggests a role for spastin phosphorylation by Cdks in the neurodegeneration of the most-common form of HSP...
  58. ncbi request reprint Truncation of CDK5 activator p35 induces intensive phosphorylation of Ser202/Thr205 of human tau
    Mitsuko Hashiguchi
    Department of Physiology, Tokyo Medical University, 6 1 1 Shinjuku, Shinjuku, Japan
    J Biol Chem 277:44525-30. 2002
    ..Considering the fact that phosphorylation of Ser(202)/Thr(205) antagonizes the tau-mediated nucleation of tubulin, p25-CDK5 may play a pivotal role in neuronal cell death in Alzheimer's disease...
  59. ncbi request reprint Differential regulation of the Cdk5-dependent phosphorylation sites of inhibitor-1 and DARPP-32 by depolarization
    Chan Nguyen
    Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    J Neurochem 103:1582-93. 2007
    ..Thus, in the striatum the regulation of phosphorylation of Cdk5-dependent sites by NMDA occurs through multiple distinct pathways...
  60. ncbi request reprint Ultra-high field NMR studies of antibody binding and site-specific phosphorylation of alpha-synuclein
    Hiroaki Sasakawa
    Department of Structural Biology and Biomolecular Engineering, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3 1 Tanabe dori, Mizuho ku, Nagoya 467 8603, Japan
    Biochem Biophys Res Commun 363:795-9. 2007
    ....
  61. doi request reprint Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS
    Yuki Inukai
    Department of Molecular Neurobiology, Tokyo Institute of Psychiatry, Tokyo Metropolitan Organization for Medical Research, 2 1 8 Kamikitazawa, Setagaya Ku, Tokyo 156 8585, Japan
    FEBS Lett 582:2899-904. 2008
    ..Analysis of postmortem changes of TDP-43 revealed that the amounts of Sarkosyl-insoluble, urea-soluble full-length TDP-43 and a 35kDa N-terminal fragment increased time-dependently...
  62. ncbi request reprint Cdk5 regulates differentiation of oligodendrocyte precursor cells through the direct phosphorylation of paxillin
    Yuki Miyamoto
    Department of Pharmacology, National Research Institute for Child Health and Development, Okura, Setagaya, Tokyo 157 8535, Japan
    J Cell Sci 120:4355-66. 2007
    ..Taken together, these results suggest that phosphorylation of paxillin by Cdk5 is a key mechanism in OL differentiation and may ultimately regulate myelination...
  63. ncbi request reprint Small molecule inhibitors of alpha-synuclein filament assembly
    Masami Masuda
    Department of Molecular Neurobiology, Tokyo Institute of Psychiatry, 2 1 8 Kamikitazawa, Setagaya Ku, Tokyo 156 8585, Japan
    Biochemistry 45:6085-94. 2006
    ....
  64. ncbi request reprint Distinct migratory behavior of early- and late-born neurons derived from the cortical ventricular zone
    Yumiko Hatanaka
    Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Aichi 444 8585, Japan
    J Comp Neurol 479:1-14. 2004
    ..Thus, there appear to be at least two distinct migratory phases of cortical neurons: one common to the early- and late-born neurons, and the other specific to late-born neurons and Cdk5-dependent...
  65. ncbi request reprint Inhibition of heparin-induced tau filament formation by phenothiazines, polyphenols, and porphyrins
    Sayuri Taniguchi
    Department of Molecular Neurobiology, Tokyo Institute of Psychiatry, Tokyo Metropolitan Organization for Medical Research, 2 1 8 Kamikitazawa, Setagaya Ku, Tokyo 156 8585, Japan
    J Biol Chem 280:7614-23. 2005
    ..Identification of small molecule inhibitors of heparin-induced assembly of tau will form a starting point for the development of mechanism-based therapies for the tauopathies...