Ikuo Hirono


Affiliation: Tokyo University of Marine Science and Technology
Country: Japan


  1. Kawato S, Shitara A, Wang Y, Nozaki R, Kondo H, Hirono I. Crustacean Genome Exploration Reveals the Evolutionary Origin of White Spot Syndrome Virus. J Virol. 2018;: pubmed publisher
    ..Our work redefines the previously poorly-characterized crustacean virus family and reveal the ancient genomic events that preordained the emergence of a devastating shrimp pathogen. ..
  2. Tinwongger S, Thawonsuwan J, Kondo H, Hirono I. Identification of an anti-lipopolysaccharide factor AV-R isoform (LvALF AV-R) related to Vp_PirAB-like toxin resistance in Litopenaeus vannamei. Fish Shellfish Immunol. 2018;84:178-188 pubmed publisher
    ..These results suggest that LvALF AV-R expression was induced in the hepatopancreas of shrimp in response to the presence of Vp_PirAB-like toxin, although other factors might also be involved in the resistance mechanism. ..
  3. Pedrosa Gerasmio I, Tanaka T, Sumi A, Kondo H, Hirono I. Effects of 5-Aminolevulinic Acid on Gene Expression, Immunity, and ATP Levels in Pacific White Shrimp, Litopenaeus vannamei. Mar Biotechnol (NY). 2018;20:829-843 pubmed publisher
    ..vannamei to V. parahaemolyticus, upregulated immune- and defense-related genes, and enhanced aerobic energy metabolism, respectively. Further studies are needed to elucidate the extent of 5-ALA use in shrimp culture. ..
  4. Kawato S, Nozaki R, Kondo H, Hirono I. Draft Genome Sequence of Vibrio penaeicida Strain TUMSAT-NU1, Isolated from Diseased Shrimp in Japan. Genome Announc. 2018;6: pubmed publisher
    ..penaeicida strain TUMSAT-NU1 consists of 100 scaffolds with a total of 6.41 Mbp. We identified possible virulence factors, and we found that V. penaeicida and Vibrio nigripulchritudo are closely related. ..
  5. Tandel G, Hipolito S, Kondo H, Hirono I. Comparative sequence analysis of crustin isoform MjCRS7 and MjWFDC-like gene from kuruma shrimp Marsupenaeus japonicus shows variant of the WFDC domain. Infect Genet Evol. 2018;64:139-148 pubmed publisher
    ..Although crustins are mainly antimicrobial peptides, the present results suggest that MjCRS7 may have other roles in M. japonicus. ..
  6. Zhang K, Koiwai K, Kondo H, Hirono I. White spot syndrome virus (WSSV) suppresses penaeidin expression in Marsupenaeus japonicus hemocytes. Fish Shellfish Immunol. 2018;78:233-237 pubmed publisher
    ..In addition, MjPen-II was observed in the cytoplasm of granule-containing hemocytes. These results suggest that WSSV suppresses MjPen-II expression in hemocytes. ..
  7. Tandel G, Kondo H, Hirono I. Gills specific type 2 crustin isoforms: Its molecular cloning and characterization from kuruma shrimp Marsupenaeus japonicus. Dev Comp Immunol. 2018;85:25-30 pubmed publisher
    ..Experimental infection was conducted against Vibrio parahaemolyticus and white spot syndrome virus (WSSV) by immersion test. However, no significant upregulation was observed. ..
  8. Kato G, Yamashita K, Kondo H, Hirono I. Protective efficacy and immune responses induced by a DNA vaccine encoding codon-optimized PPA1 against Photobacterium damselae subsp. piscicida in Japanese flounder. Vaccine. 2015;33:1040-5 pubmed publisher
    ..These data show that codon-optimized DNA vaccine pPPA1(opt) had a strong immunogenicity and conferred protective efficacy against Pdp infection in Japanese flounder. ..
  9. Zhang K, Koiwai K, Kondo H, Hirono I. A novel white spot syndrome virus-induced gene (MjVIG1) from Marsupenaeus japonicus hemocytes. Fish Shellfish Immunol. 2018;77:46-52 pubmed publisher
    ..In contrast, MjVIG1 transcript level did not change after YHV and V. parahaemolyticus infection. These results indicated that MjVIG1 might be a WSSV-specific induced gene in M. japonicus hemocytes. ..

More Information


  1. Zhao B, Katagiri T, Kondo H, Hirono I. Comparative analysis of two types of CXCL8 from Japanese flounder (Paralichthys olivaceus). Dev Comp Immunol. 2015;52:37-47 pubmed publisher
    ..The transcripts of IgM only significantly increased on day 7 post-injection of pCI-CXCL8_L1b. ..
  2. Koiwai K, Kondo H, Hirono I. The immune functions of sessile hemocytes in three organs of kuruma shrimp Marsupenaeus japonicus differ from those of circulating hemocytes. Fish Shellfish Immunol. 2018;78:109-113 pubmed publisher
  3. Koiwai K, Kondo H, Hirono I. RNA-seq identifies integrin alpha of kuruma shrimp Marsupenaeus japonicus as a candidate molecular marker for phagocytic hemocytes. Dev Comp Immunol. 2018;81:271-278 pubmed publisher
    ..This study presents Mj-Intgα as a candidate molecular marker for future functional characterization of hemocytes. ..
  4. Matsumoto T, Feroudj H, Kikuchi R, Kawana Y, Kondo H, Hirono I, et al. DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin. Microarrays (Basel). 2014;3:226-44 pubmed publisher
    ..In conclusion, our results demonstrate that the intramuscular administration of TTX changes the expression of hepatic genes involved in various signaling pathways. ..
  5. Koiwai K, Alenton R, Kondo H, Hirono I. Extracellular trap formation in kuruma shrimp (Marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme. Fish Shellfish Immunol. 2016;52:206-9 pubmed publisher
    ..Fluorescence imaging revealed that c-type lysozyme proteins were released around the ET complex after E. coli stimulation, suggesting the presence of a coupled antimicrobial immune response involving ET formation and AMP release. ..
  6. Elbahnaswy S, Koiwai K, Zaki V, Shaheen A, Kondo H, Hirono I. A novel viral responsive protein (MjVRP) from Marsupenaeus japonicus haemocytes is involved in white spot syndrome virus infection. Fish Shellfish Immunol. 2017;70:638-647 pubmed publisher
    ..These results suggest that MjVRP+ haemocytes have a supporting role in the pathogenesis of WSSV. ..
  7. Koiwai K, Alenton R, Shiomi R, Nozaki R, Kondo H, Hirono I. Two hemocyte sub-populations of kuruma shrimp Marsupenaeus japonicus. Mol Immunol. 2017;85:1-8 pubmed publisher
  8. Kondo H, Van P, Dang L, Hirono I. Draft Genome Sequence of Non-Vibrio parahaemolyticus Acute Hepatopancreatic Necrosis Disease Strain KC13.17.5, Isolated from Diseased Shrimp in Vietnam. Genome Announc. 2015;3: pubmed publisher
    ..Normally, AHPND is caused by Vibrio parahaemolyticus, but the genomic sequence of the strain indicated that it belonged to Vibrio harveyi. The sequence data included plasmid-like sequences and putative virulence genes. ..