E H Chowdhury

Summary

Affiliation: Tokyo Institute of Technology
Country: Japan

Publications

  1. ncbi request reprint High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca-Mg phosphate
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Gene 341:77-82. 2004
  2. ncbi request reprint pH-sensing nano-crystals of carbonate apatite: effects on intracellular delivery and release of DNA for efficient expression into mammalian cells
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Gene 376:87-94. 2006
  3. ncbi request reprint Self-assembly of DNA and cell-adhesive proteins onto pH-sensitive inorganic crystals for precise and efficient transgene delivery
    E H Chowdhury
    Global Edge Institute, Tokyo Institute of Technology, 2 12 1, E3 15, Ookayama, Meguro, Tokyo 152 8550, Japan
    Curr Pharm Des 14:2212-28. 2008
  4. ncbi request reprint Protein kinase C activation enhances transfection efficacy of cell-adhesive protein-anchored carbonate apatite nanocrystals
    K Kutsuzawa
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 371:116-7. 2007
  5. doi request reprint Synergistic effect of PKC activation and actin filament disruption on carbonate apatite-facilitated lymphocyte transfection
    K Fukuda
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan
    Biochem Biophys Res Commun 419:482-4. 2012
  6. doi request reprint Disrupting actin filaments promotes efficient transfection of a leukemia cell line using cell adhesive protein-embedded carbonate apatite particles
    K Kutsuzawa
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 388:164-6. 2009
  7. ncbi request reprint Fibronectin-coated nano-precipitates of calcium-magnesium phosphate for integrin-targeted gene delivery
    E H Chowdhury
    School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta cho, Midori ku, Yokohama, Japan
    J Control Release 116:e68-9. 2006
  8. pmc High performance DNA nano-carriers of carbonate apatite: multiple factors in regulation of particle synthesis and transfection efficiency
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama, Japan
    Int J Nanomedicine 2:101-6. 2007
  9. ncbi request reprint Apatite with less crystallinity is a superior agent for macromolecular drug delivery to mammalian cells
    E H Chowdhury
    School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta cho, Midori ku, Yokohama, Japan
    J Control Release 116:e42-3. 2006
  10. ncbi request reprint Integrin-supported fast rate intracellular delivery of plasmid DNA by extracellular matrix protein embedded calcium phosphate complexes
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Biochemistry 44:12273-8. 2005

Collaborators

Detail Information

Publications19

  1. ncbi request reprint High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca-Mg phosphate
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Gene 341:77-82. 2004
    ....
  2. ncbi request reprint pH-sensing nano-crystals of carbonate apatite: effects on intracellular delivery and release of DNA for efficient expression into mammalian cells
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Gene 376:87-94. 2006
    ..Thus we have developed an innovative technology with significant insights, that would come as a promising tool for both basic research laboratories and clinical settings...
  3. ncbi request reprint Self-assembly of DNA and cell-adhesive proteins onto pH-sensitive inorganic crystals for precise and efficient transgene delivery
    E H Chowdhury
    Global Edge Institute, Tokyo Institute of Technology, 2 12 1, E3 15, Ookayama, Meguro, Tokyo 152 8550, Japan
    Curr Pharm Des 14:2212-28. 2008
    ..Thus, the new establishment of a bio-functional hybrid gene-carrier would promote and facilitate development of stem cell-based therapy in regenerative medicine...
  4. ncbi request reprint Protein kinase C activation enhances transfection efficacy of cell-adhesive protein-anchored carbonate apatite nanocrystals
    K Kutsuzawa
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 371:116-7. 2007
  5. doi request reprint Synergistic effect of PKC activation and actin filament disruption on carbonate apatite-facilitated lymphocyte transfection
    K Fukuda
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan
    Biochem Biophys Res Commun 419:482-4. 2012
    ....
  6. doi request reprint Disrupting actin filaments promotes efficient transfection of a leukemia cell line using cell adhesive protein-embedded carbonate apatite particles
    K Kutsuzawa
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 388:164-6. 2009
    ..Moreover, transgene expression efficiency could be enhanced dramatically with the cell adhesive protein-embedded particles finally up to 150 times by selectively disrupting the actin filaments...
  7. ncbi request reprint Fibronectin-coated nano-precipitates of calcium-magnesium phosphate for integrin-targeted gene delivery
    E H Chowdhury
    School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta cho, Midori ku, Yokohama, Japan
    J Control Release 116:e68-9. 2006
  8. pmc High performance DNA nano-carriers of carbonate apatite: multiple factors in regulation of particle synthesis and transfection efficiency
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama, Japan
    Int J Nanomedicine 2:101-6. 2007
    ....
  9. ncbi request reprint Apatite with less crystallinity is a superior agent for macromolecular drug delivery to mammalian cells
    E H Chowdhury
    School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta cho, Midori ku, Yokohama, Japan
    J Control Release 116:e42-3. 2006
  10. ncbi request reprint Integrin-supported fast rate intracellular delivery of plasmid DNA by extracellular matrix protein embedded calcium phosphate complexes
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Biochemistry 44:12273-8. 2005
    ....
  11. ncbi request reprint Surface functionalization of inorganic nano-crystals with fibronectin and E-cadherin chimera synergistically accelerates trans-gene delivery into embryonic stem cells
    K Kutsuzawa
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Biochem Biophys Res Commun 350:514-20. 2006
    ..Thus, the new establishment of a bio-functional hybrid gene-carrier would promote and facilitate development of stem cell-based therapy in regenerative medicine...
  12. ncbi request reprint Effective delivery with enhanced translational activity synergistically accelerates mRNA-based transfection
    F T Zohra
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Biochem Biophys Res Commun 358:373-8. 2007
    ..Our findings, therefore, unveiled a novel approach that an effective delivery system can be developed by the improvement of the gene expression level in combination with the enhancement of the carrier potency...
  13. ncbi request reprint Efficient transfection of mouse embryonic stem cells with cell-adhesive protein-embedded inorganic nanocarrier
    K Kutsuzawa
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 372:122-4. 2008
  14. ncbi request reprint Drastic effect of nanoapatite particles on liposome-mediated mRNA delivery to mammalian cells
    F T Zohra
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 345:164-6. 2005
  15. ncbi request reprint Fibronectin in collaboration with Mg2+ enhances transgene expression by calcium phosphate coprecipitates
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 335:162-4. 2004
  16. ncbi request reprint Dramatic effect of Mg2+ on transfecting mammalian cells by DNA/calcium phosphate precipitates
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 328:96-7. 2004
  17. ncbi request reprint A bio-recognition device developed onto nano-crystals of carbonate apatite for cell-targeted gene delivery
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Biotechnol Bioeng 90:414-21. 2005
    ....
  18. ncbi request reprint Rapid isolation of high quality, multimeric plasmid DNA using zwitterionic detergent
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    J Biotechnol 119:343-7. 2005
    ..Thus, considering both its remarkable simplicity and efficiency in producing sufficiently pure plasmid DNA, the new strategy would emerge a useful tool in modern recombinant technology and therapeutic applications...
  19. ncbi request reprint Transfecting mammalian cells by DNA/calcium phosphate precipitates: effect of temperature and pH on precipitation
    E H Chowdhury
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Anal Biochem 314:316-8. 2003