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Publications
Iron-regulated haemolysin gene from Edwardsiella tardaI Hirono
Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato ku, Japan
Mol Microbiol 24:851-6. 1997..The ethA and ethB genes were very prevalent in haemolytic E. tarda strains isolated from diseased fish. Transcription of the ethB gene was regulated by iron. The ethA and ethB genes were transcribed independently...- Elevated serum vitellogenin levels and gonadal abnormalities in wild male flounder (Pleuronectes yokohamae) from Tokyo Bay, JapanS Hashimoto
Department of Ocean Sciences, Tokyo University of Fisheries, 4 5 7 Konan, Minato ku, Tokyo 108 8477, Japan
Mar Environ Res 49:37-53. 2000..This study suggests that the abnormally high levels of VTG and the presence of oocytes in the testis of male flounder collected from Tokyo Bay could be the effect of environmental estrogens... - Establishment of a sampling strategy for the use of blue mussels as an indicator of organotin contamination in the coastal environmentK Shindo
Department of Marine Science and Technology, Tokyo University of Fisheries, 4 5 7 Konan, Minato ku, Tokyo 108, Japan
J Environ Monit 1:243-50. 1999..For monitoring sites where natural blue mussels cannot be collected, an alternative method of transplanting blue mussels from a relatively clean area would be required... - Histamine formation by Tetragenococcus muriaticus, a halophilic lactic acid bacterium isolated from fish sauceB Kimura
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Int J Food Microbiol 70:71-7. 2001..A conspicuous amount of histamine accumulated only during the late stationary phase regardless of the growth conditions. Studies of cell suspension experiments confirmed the results obtained from cultured cells... - Properties of proteinaceous toxins in the salivary gland of the marine gastropod (Monoplex echo)Kazuo Shiomi
Department of Food Science and Technology, Tokyo University of Fisheries, Konan 4, Minato ku, 108 8477, Tokyo, Japan
Toxicon 40:563-71. 2002..It is thus concluded that echotoxins injected into mice induce rapid hemolysis by binding to receptors (possibly gangliosides) gangliosides in the erythrocyte membrane, eventually leading to death of mice... - A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatusHiroshi Nagai
Suntory Institute for Bioorganic Research, Mishima, Osaka, Japan
Biosci Biotechnol Biochem 66:97-102. 2002..The deduced amino acid sequence of CqTX-A (462 amino acids) showed 25.2% and 21.6% sequence similarity to Carybdea rastoni toxins (CrTXs) and Carybdea alata toxin-A (CrTX-A), respectively, which are Cubozoan jellyfish toxins... - Fractal analysis of ice crystals in frozen foodTomoaki Hagiwara
Department of Food Science and Technology, Tokyo University of Fisheries, 4 5 7 Konan, Minato ku, Tokyo 108 8477, Japan
J Agric Food Chem 50:3085-9. 2002.... - Novel proteinaceous toxins from the nematocyst venom of the Okinawan sea anemone Phyllodiscus semoni KwietniewskiHiroshi Nagai
Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka 618 8503, Japan
Biochem Biophys Res Commun 294:760-3. 2002..The N-terminal amino acid sequence of PsTX-60B showed homology with that of PsTX-60A. These toxins represent a novel class of cytolytic proteinaceous toxins... - Effective structure of a leader open reading frame for enhancing the expression of GC-rich genesMasami Ishida
Laboratory of Marine Biochemistry, Tokyo University of Fisheries, 4 5 7 Konan, Minato ku, Tokyo 108 8477, Japan
J Biochem 132:63-70. 2002..Using the most effective leader ORF obtained from these results, new expression vectors were constructed... - A tetrodotoxin-binding protein in the hemolymph of shore crab Hemigrapsus sanguineus: purification and propertiesYuji Nagashima
Department of Food Science and Technology, Tokyo University of Fisheries, Konan, Minato, Tokyo 108 8477, Japan
Toxicon 40:753-60. 2002.... - Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosusMasami Ishida
Laboratory of Marine Biochemistry, Tokyo University of Fisheries, Konan 4, Minato ku, Tokyo 108 8477, Japan
FEMS Microbiol Lett 216:179-83. 2002..The expression of the trpA gene was also improved ( approximately 1.5-fold). This approach may be useful for overexpressing various kinds of AT-rich genes... - An epidermal growth factor-like toxin and two sodium channel toxins from the sea anemone Stichodactyla giganteaKazuo Shiomi
Department of Food Science and Technology, Tokyo University of Fisheries, Konan 4, Minato ku, 108 8477, Tokyo, Japan
Toxicon 41:229-36. 2003..Gigantoxin I is the first example of EGF-like toxins of natural origin... - A new polypeptide toxin from the nematocyst venom of an Okinawan sea anemone Phyllodiscus semoni (Japanese name "unbachi-isoginchaku")Hiroshi Nagai
Suntory Institute for Bioorganic Research, 1 1 1 Wakayamadai, Shimamoto, Mishima, Osaka 618 8503, Japan
Biosci Biotechnol Biochem 66:2621-5. 2002..PsTX-20A showed lethal toxicity to the shrimp Palaemon paucidens when administered via intraperitoneal injection (LD50, 50 microg/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED50, 80 ng/ml)... - In vitro accumulation of tetrodotoxin in pufferfish liver tissue slicesYuji Nagashima
Department of Food Science and Technology, Tokyo University of Fisheries, Konan, Minato, Tokyo 108 8477, Japan
Toxicon 41:569-74. 2003..These results suggest that the difference in the accumulation of TTX between pufferfish and filefish livers is ascribable to the difference not in the TTX excreting ability but in the ability to take up TTX... - Purification and characterization of an antibacterial protein in the skin secretion of rockfish Sebastes schlegeliY Nagashima
Department of Food Science and Technology, Tokyo University of Fisheries, Minato, 108 8477, Tokyo, Japan
Comp Biochem Physiol C Toxicol Pharmacol 136:63-71. 2003..The potent sensitivity against virulent pathogens such as A. hydrophila, A. salmonicida and P. damselae may contribute considerably to the innate host defense mechanism to combat microbes on the mucosal surfaces of the rockfish... - Three new chlorine containing antibiotics from a marine-derived fungus Aspergillus ostianus collected in PohnpeiMichio Namikoshi
Department of Ocean Sciences, Tokyo University of Fisheries, Minato ku, Tokyo 108 8477, Japan
J Antibiot (Tokyo) 56:755-61. 2003..Compound 1 inhibited the growth of R. atlantica at 5 microg/disc (inhibition zone: 12.7 mm), while 2 and 3 were active at 25 microg/disc (10.1 and 10.5 mm, respectively)... - Isolation and characterization of halophilic lactic acid bacteria isolated from "terasi" shrimp paste: a traditional fermented seafood product in IndonesiaTakeshi Kobayashi
Department of Food Science and Technology, Tokyo University of Fisheries, Minato ku, Tokyo 108 8477, Japan
J Gen Appl Microbiol 49:279-86. 2003..This study is the first to show that both species of Tetragenococcus are distributed in Indonesian fermented foods... - Heat-induced germ cell deficiency in the teleosts Odontesthes bonariensis and Patagonina hatcheriC A Strüssmann
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan
Comp Biochem Physiol A Mol Integr Physiol 119:637-44. 1998..The potential implications of these findings for physiological, ecological, and environmental studies and the usefulness of heat-induced germ cell-deficient fish in research and animal production are discussed... - Rapid, quantitative PCR monitoring of growth of Clostridium botulinum type E in modified-atmosphere-packaged fishB Kimura
Tokyo University of Fisheries, Department of Food Science and Technology, Tokyo 108 8477, Japan
Appl Environ Microbiol 67:206-16. 2001..These results suggested that quantification of BoNT/E DNA by the rapid, quantitative PCR method was a good method for the sensitive assessment of botulinal risk in the seafood samples tested... - Expressed sequence tags of medaka (Oryzias latipes) liver mRNAI Hirono
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan
Mol Mar Biol Biotechnol 6:345-50. 1997..Sequences of 10 of the 18 clones had not previously been reported in fish. The codon usage of medaka genes is similar to that of Xenopus, mouse, and human genes, but not similar to that of yeast and Escherichia coli genes... - Seven new microcystins possessing two L-glutamic acid units, isolated from Anabaena sp. strain 186M Namikoshi
Department of Ocean Sciences, Tokyo University of Fisheries, Japan
Chem Res Toxicol 11:143-9. 1998..These microcystins are the first examples containing dicarboxylic amino acids at the two variable L-amino acid units in microcystins... - Evaluation of TaqMan PCR assay for detecting Salmonella in raw meat and shrimpB Kimura
Department of Food Science and Technology, Tokyo University of Fisheries, Minato, Japan
J Food Prot 62:329-35. 1999..These results indicate that TaqMan is a reliable and rapid method for Salmonella analysis in the food industry. With this system, food samples can be analyzed for Salmonella in less than 20 h... - Subcellular distribution of tetrodotoxin in puffer fish liverY Nagashima
Department of Food Science and Technology, Tokyo University of Fisheries, Minato, Japan
Toxicon 37:1833-7. 1999..These results reveal that tetrodotoxin is widely distributed in organelles in liver cells, though predominantly in the cytosol fraction... - Isolation and structures of grammistins, peptide toxins from the skin secretion of the soapfish Grammistes sexlineatusK Shiomi
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Toxicon 38:91-103. 2000..Interestingly, grammistin Gs 2 is much more abundant in amphiphilic alpha-helices and much higher in biological activities than melittin and pardaxins as well as grammistin Cs 1... - Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineageG Yoshizaki
Department of Aquatic Biosciences, Tokyo University of Fisheries, Tokyo, Japan
Mol Reprod Dev 55:364-71. 2000..This marker will make it possible to identify the PGCs or presumptive PGCs in early trout embryos whose germ cells can not be distinguished by morphological characteristics... - Strictly anaerobic halophiles isolated from canned Swedish fermented herrings (Surströmming)T Kobayashi
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Int J Food Microbiol 54:81-9. 2000..This is a novel report of halophilic anaerobes isolated from a food product. Such anaerobes may contribute to the intense flavor and the swollen can characteristics of Swedish fermented herring... - Increase of hybrids in yeast electrofusion by mitochondrial mutationsN Urano
Laboratory of Marine Biochemistry, Tokyo University of Fisheries, 5 7 Konan 4, Minato, Tokyo 108 8477, Japan
Biotechnol Bioeng 69:113-6. 2000..Yields of the hybrids in system (2) were over 100-fold higher than those in system (1). Thus, regulation of yeast electrofusion was found to be possible by mitochondrial mutations... - Photobacterium histaminum Okuzumi et al. 1994 is a later subjective synonym of Photobacterium damselae subsp. damselae (Love et al. 1981) Smith et al. 1991B Kimura
Department of Food Science and Technology, Tokyo University of Fisheries, Konan, Minato, Japan
Int J Syst Evol Microbiol 50:1339-42. 2000..Therefore, the name P. histaminum should be considered to be a later subjective synonym of P. damselae subsp. damselae... - Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoterG Yoshizaki
Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan, Japan
Int J Dev Biol 44:323-6. 2000..This transgenic trout, having visualizable PGCs, will make it possible to isolate live PGCs for in vitro studies and to study the ontogeny of PGCs including sex differentiation in live embryos... - Differentiation of Tetragenococcus populations occurring in products and manufacturing processes of puffer fish ovaries fermented with rice-branT Kobayashi
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Int J Food Microbiol 56:211-8. 2000..Physiologically, the major differences between the two groups were found in the ability to grow in medium not supplemented with NaCl and the fermentation of L-arabinose, sucrose and D-mannitol, and several other carbohydrates... - Haloanaerobium fermentans sp. nov., a strictly anaerobic, fermentative halophile isolated from fermented puffer fish ovariesT Kobayashi
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Int J Syst Evol Microbiol 50:1621-7. 2000..Based on these results, the new isolate appears to represent a new Haloanaerobium species, for which the name Haloanaerobium fermentans sp. nov. is proposed. The type strain is R-9T (= JCM 10494T)... - Occurrence of tetrodotoxin-related substances in the nontoxic puffer Takifugu xanthopterusY Nagashima
Department of Food Science and Technology, Tokyo University of Fisheries, Minato, Japan
Toxicon 39:415-8. 2001..Our results suggest that nontoxic puffer fish contains nontoxic tetrodotoxin derivatives as precursors or metabolites of tetrodotoxin... - Isolation and characterization of bioactive metabolites from marine-derived filamentous fungi collected from tropical and sub-tropical coral reefsM Namikoshi
Department of Ocean Sciences, Tokyo University of Fisheries, Japan
Chem Pharm Bull (Tokyo) 48:1452-7. 2000..The utility of marine-derived fungi as a source of bioactive secondary metabolites is discussed... - Identification of viral induced genes in Ig+ leucocytes of Japanese flounder Paralichthys olivaceus, by differential hybridisation with subtracted and un-subtracted cDNA probesT Aoki
Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Japan
Fish Shellfish Immunol 10:623-30. 2000..From leucocytes mRNA for homologues of IFI56, CEF-10, Stat3, SERPIN and inducible poly (A) binding protein expression was shown to increase following HRV infection... - Empirical Bayes procedure for estimating genetic distance between populations and effective population sizeS Kitada
Department of Aquatic Biosciences, Tokyo University of Fisheries, Minato, Tokyo 108 8477, Japan
Genetics 156:2063-79. 2000..The joint marginal-likelihood function also estimates the rate of gene flow into island populations... - Recent topics in NMR imaging and MRITokuko Watanabe
Department of Food Science and Technology, Tokyo University of Fisheries, Japan
Magn Reson Med Sci 1:38-49. 2002....