M Ogura

Summary

Affiliation: Tokai University
Country: Japan

Publications

  1. ncbi request reprint Identification of the sequences recognized by the Bacillus subtilis response regulator YrkP
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Orido Shimizu, Shizuoka, Japan
    Biosci Biotechnol Biochem 72:186-96. 2008
  2. doi request reprint SwrA regulates assembly of Bacillus subtilis DegU via its interaction with N-terminal domain of DegU
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Shizuoka, Japan
    J Biochem 151:643-55. 2012
  3. pmc Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB
    Kensuke Tsukahara
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    BMC Microbiol 8:8. 2008
  4. ncbi request reprint Bacillus subtilis rapD, a direct target of transcription repression by RghR, negatively regulates srfA expression
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Shizuoka, Japan
    FEMS Microbiol Lett 268:73-80. 2007
  5. ncbi request reprint The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    Microbiology 153:667-75. 2007
  6. ncbi request reprint Formation of 4-vinyl guaiacol as an intermediate in bioconversion of ferulic acid by Schizophyllum commune
    Sho ichi Tsujiyama
    Biological Function Science Course, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan
    Biosci Biotechnol Biochem 72:212-5. 2008
  7. pmc The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD)
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    J Bacteriol 191:949-58. 2009
  8. ncbi request reprint Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis
    Mitsuo Ogura
    Department of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424 8610, Japan
    Mol Microbiol 49:1685-97. 2003
  9. doi request reprint Autoregulation of the Bacillus subtilis response regulator gene degU is coupled with the proteolysis of DegU-P by ClpCP
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido, Shizuoka 424 8610, Japan
    Mol Microbiol 75:1244-59. 2010
  10. ncbi request reprint Identification of the sequences recognized by the Bacillus subtilis response regulator YclJ
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    Arch Microbiol 192:569-80. 2010

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Identification of the sequences recognized by the Bacillus subtilis response regulator YrkP
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Orido Shimizu, Shizuoka, Japan
    Biosci Biotechnol Biochem 72:186-96. 2008
    ..lacZ fusion analysis of deleted and mutagenized promoter regions of yrkO and yrkPQR divergon confirmed that the three YrkP-binding regions are needed for the YrkP-mediated activation of yrkO and/or yrkPQR...
  2. doi request reprint SwrA regulates assembly of Bacillus subtilis DegU via its interaction with N-terminal domain of DegU
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Shizuoka, Japan
    J Biochem 151:643-55. 2012
    ..Together, these results demonstrate that SwrA can stabilize the binding of DegU to the ycdA promoter with low affinity. Thus, SwrA is a novel type of bacterial transcription factor in this regard...
  3. pmc Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB
    Kensuke Tsukahara
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    BMC Microbiol 8:8. 2008
    ..subtilis. Although phosphorylated DegU (DegU-P) regulates more than one hundred and twenty genes, the targets of unphosphorylated DegU are unknown, except for comK...
  4. ncbi request reprint Bacillus subtilis rapD, a direct target of transcription repression by RghR, negatively regulates srfA expression
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, Shizuoka, Japan
    FEMS Microbiol Lett 268:73-80. 2007
    ..DNA microarray analysis and gel retardation assays revealed that rapD expression is directly repressed by RghR. Thus, the ComA regulon is regulated by rapD in a SigX- and RghR-dependent manner...
  5. ncbi request reprint The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    Microbiology 153:667-75. 2007
    ..Deleted and mutagenized promoter regions of natA were analysed using a lacZ fusion, and it was confirmed that the direct repeat is critical for natA activation by NatR...
  6. ncbi request reprint Formation of 4-vinyl guaiacol as an intermediate in bioconversion of ferulic acid by Schizophyllum commune
    Sho ichi Tsujiyama
    Biological Function Science Course, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan
    Biosci Biotechnol Biochem 72:212-5. 2008
    ..commune converted ferulic acid into 4-vinyl guaiacol by decarboxylation. This was then oxidized to vanillin and vanillic acid. This result indicates that S. commune distinguished ferulic acid from lignins and metabolized it specifically...
  7. pmc The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD)
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    J Bacteriol 191:949-58. 2009
    ..Therefore, yutB and comN regulate comE at the transcription and post-transcription initiation levels, respectively. These results demonstrate that a comE-specific regulatory mechanism is involved in development of genetic competence...
  8. ncbi request reprint Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis
    Mitsuo Ogura
    Department of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424 8610, Japan
    Mol Microbiol 49:1685-97. 2003
    ..Gel shift analysis revealed that RapG served as the inhibitor of DegU binding to the promoter regions of aprE and comK and that this inhibition was counteracted by the PhrG peptide...
  9. doi request reprint Autoregulation of the Bacillus subtilis response regulator gene degU is coupled with the proteolysis of DegU-P by ClpCP
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido, Shizuoka 424 8610, Japan
    Mol Microbiol 75:1244-59. 2010
    ..We confirmed that DegU-P was degraded preferentially using an in vitro ClpCP degradation system. Furthermore, a mutational analysis showed that the N-terminal region of DegU is important for proteolysis...
  10. ncbi request reprint Identification of the sequences recognized by the Bacillus subtilis response regulator YclJ
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    Arch Microbiol 192:569-80. 2010
    ..Furthermore, we identified two more YclJ-regulated genes, yycA and yfjR, using bioinformatic analysis of the B. subtilis genome, and it was shown that YclJ binds to those promoters and controls the expression of those genes...
  11. ncbi request reprint Med, a cell-surface localized protein regulating a competence transcription factor gene, comK, in Bacillus subtilis
    Mitsuo Ogura
    Department of Marine Science and Technology, Tokai University, Shimizu, Shizuoka, Japan
    Biosci Biotechnol Biochem 66:892-6. 2002
    ..The results obtained are consistent with the localization of Med at the cell surface. An implication of the cell-surface localization of Med is discussed in terms of comK regulation...
  12. pmc Bacillus subtilis comZ (yjzA) negatively affects expression of comG but not comK
    M Ogura
    Department of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424, Japan
    J Bacteriol 182:4992-4. 2000
    ..The expression of comK and the other three late competence operons was not affected significantly in the yjzA-deficient mutant...
  13. doi request reprint ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis
    Mitsuo Ogura
    Institute of Oceanic Research and Development, Tokai University, 3 20 1 Orido Shimizu, Shizuoka 424 8610, Japan
    J Biochem 150:615-25. 2011
    ..Taken together, we propose that the both ZnuABC- and ZosA-mediated zinc incorporation is involved in competence development, although the two zinc transporters are differently implicated in this developmental process...
  14. pmc A new Bacillus subtilis gene, med, encodes a positive regulator of comK
    M Ogura
    School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka, Japan
    J Bacteriol 179:6244-53. 1997
    ..An epistatic analysis revealed that overproduction of ComK resulted in alteration of med expression, suggesting a regulatory loop between comK and med. Several possible mechanisms for positive regulation of comK by Med are discussed...
  15. pmc DNA microarray analysis of Bacillus subtilis DegU, ComA and PhoP regulons: an approach to comprehensive analysis of B.subtilis two-component regulatory systems
    M Ogura
    Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka 424-8610, Japan
    Nucleic Acids Res 29:3804-13. 2001
    ..These results suggest the applicability of the strategy to the comprehensive transcription analysis of the B.subtilis two-component systems...
  16. doi request reprint Efficacy and safety of switching from basal insulin to sitagliptin in Japanese type 2 diabetes patients
    S I Harashima
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Horm Metab Res 45:231-8. 2013
    ..19. In conclusion, basal insulin in BOT can be replaced by sitagliptin with a decrease in HbA1c level and frequency of hypoglycemia in cases where insulin secretion capacity was sufficiently preserved...
  17. ncbi request reprint A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis
    T Tanaka
    Department of Marine Science, School of Marine Science and Technology, University of Tokai, Shimizu, Shizuoka, Japan
    FEBS Lett 422:243-6. 1998
    ..These features show that pLS32 is a novel plasmid capable of replication in B. subtilis...
  18. pmc Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis
    M Ogura
    School of Marine Science and Technology, Tokai University, Shizuoka, Japan
    J Bacteriol 176:5673-80. 1994
    ..Possible involvement of DegR in this process is discussed...
  19. pmc Involvement of stringent factor RelA in expression of the alkaline protease gene aprE in Bacillus subtilis
    M Hata
    Department of Marine Science, School of Marine Science and Technology, University of Tokai, Shimizu, Shizuoka 424-8610, Japan
    J Bacteriol 183:4648-51. 2001
    ....
  20. ncbi request reprint Phase I and pharmacokinetic study of oral fludarabine phosphate in relapsed indolent B-cell non-Hodgkin's lymphoma
    Y Ogawa
    Department of Hematology and Oncology, Tokai University School of Medicine, Isehara, and Hematology Division, National Cancer Center Hospital, Tokyo, Japan
    Ann Oncol 17:330-3. 2006
    ..The primary objective of this study was to investigate the tolerability, efficacy and pharmacokinetic profile of oral fludarabine phosphate in relapsed patients with indolent B-cell non-Hodgkin's lymphoma (B-NHL)...
  21. ncbi request reprint Phase III study to evaluate the use of high-dose chemotherapy as consolidation of treatment for high-risk postoperative breast cancer: Japan Clinical Oncology Group study, JCOG 9208
    Yutaka Tokuda
    Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259 1193, Japan
    Cancer Sci 99:145-51. 2008
    ..78). Although the prespecified values of the two arms were not so accurate as to allow detection of the observed difference, no advantage of HDC was observed in terms of RFS or overall survival...
  22. doi request reprint Phase III trial of CHOP-21 versus CHOP-14 for aggressive non-Hodgkin's lymphoma: final results of the Japan Clinical Oncology Group Study, JCOG 9809
    K Ohmachi
    Division of Hematology, Department of Internal Medicine, Tokai University, Isehara, Kanagawa, Japan
    Ann Oncol 22:1382-91. 2011
    ..We conducted a phase III trial to determine whether dose-dense strategy involving interval shortening of CHOP (CHOP-14) is superior to CHOP-21...
  23. doi request reprint Sitagliptin add-on to low dosage sulphonylureas: efficacy and safety of combination therapy on glycaemic control and insulin secretion capacity in type 2 diabetes
    S I Harashima
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Int J Clin Pract 66:465-76. 2012
    ..To assess the efficacy and safety of combination therapy with sitagliptin and low dosage sulphonylureas on glycaemic control and insulin secretion capacity in Japanese type 2 diabetes...
  24. ncbi request reprint Metformin suppresses hepatic gluconeogenesis and lowers fasting blood glucose levels through reactive nitrogen species in mice
    Y Fujita
    Department of Diabetes and Clinical Nutrition, Graduate School of Medicine, Kyoto University, 54 Shogoin, Kawahara cho, Sakyo ku, Kyoto, 606 8507, Japan
    Diabetologia 53:1472-81. 2010
    ..To investigate AMPK activation by metformin in liver, we examined the role of reactive nitrogen species (RNS) in suppression of hepatic gluconeogenesis...
  25. ncbi request reprint Superoxide stress decreases expression of srfA through inhibition of transcription of the comQXP quorum-sensing locus in Bacillus subtilis
    Taku Ohsawa
    Department of Marine Science, School of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424 8610, Japan
    J Biochem 139:203-11. 2006
    ..In support of this conclusion, the effect of added paraquat was significantly alleviated in a comX-independent srfA expression system...
  26. pmc Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK
    Mitsuo Ogura
    School of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424 8610, Japan
    J Bacteriol 184:2344-51. 2002
    ..Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK...
  27. ncbi request reprint Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis
    Hideaki Nanamiya
    Laboratory of Molecular Genetics, College of Science, Rikkyo St Paul s University, Toshima ku, Tokyo 171 8501, Japan
    J Biochem 133:295-302. 2003
    ..These results indicate that ClpX plays an important role in the regulation of ComK at the post-transcriptional level...
  28. ncbi request reprint Inhibition of Bacillus subtilis aprE expression by lincomycin at the posttranscriptional level through inhibition of ppGpp synthesis
    Atsuhiro Arai
    Department of Marine Science, School of Marine Science and Technology, University of Tokai, Shimizu Orido 3 20 1, Shimizu, Shizuoka 424 8610
    J Biochem 134:691-7. 2003
    ..From these results, we conclude that aprE expression is controlled by the stringent control at the posttranscriptional level, and that Lm inhibits this process by inhibiting ppGpp synthesis on the ribosome...
  29. pmc Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE
    Mitsuo Ogura
    Department of Marine Science, School of Marine Science and Technology, Tokai University, Shizuoka 424 8610, Japan
    J Bacteriol 186:3056-64. 2004
    ..From these results we conclude that SalA negatively regulates scoC expression. It was also found that the expression of a salA-lacZ fusion was increased by salA deficiency, suggesting that salA is autoregulated...
  30. doi request reprint Characterization of DegU-dependent expression of bpr in Bacillus subtilis
    Kensuke Tsukahara
    Institute of Oceanic Research and Development, Tokai University, Orido Shimizu, Shizuoka, Japan
    FEMS Microbiol Lett 280:8-13. 2008
    ..Mutational analysis using a bpr-lacZ fusion revealed that the three direct repeats in bpr are needed for DegU-dependent transcription activation...
  31. ncbi request reprint Mutational analysis of the helix-turn-helix region of Bacillus subtilis response regulator DegU, and identification of cis-acting sequences for DegU in the aprE and comK promoters
    Kana Shimane
    Department of Marine Science and Technology, Tokai University, 3 20 1 Shimizu Orido, Shizuoka 424 8610, Japan
    J Biochem 136:387-97. 2004
    ..Based on these results, it was concluded that DegU would function through the inverted repeat in the comK promoter and the tandem repeat in the aprE promoter...
  32. pmc The H2O2 stress-responsive regulator PerR positively regulates srfA expression in Bacillus subtilis
    Kentaro Hayashi
    Department of Marine Science, School of Marine Science and Technology, Tokai University, 3 20 1 Orido, Shimizu, Shizuoka 424 8610, Japan
    J Bacteriol 187:6659-67. 2005
    ..A transcriptional srfA-lacZ fusion lacking both PerR boxes showed diminished and PerR-independent expression, indicating that the PerR boxes we identified function as positive cis elements for srfA transcription...
  33. ncbi request reprint Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon
    Kentaro Hayashi
    Department of Marine Science and Technology, Tokai University, Shizuoka, Japan
    Mol Microbiol 59:1714-29. 2006
    ..Thus, YvaN was renamed RghR (rapG and rapH repressor). As the rapH gene is activated by ComK and RapH inhibits comK indirectly, this constitutes an autoregulatory loop modulated by RghR...
  34. ncbi request reprint Recent progress in Bacillus subtilis two-component regulation
    Mitsuo Ogura
    Department of Marine Science, School of Marine Science and Technology, Tokai University, Orido 3 20 1, Shimizu, Shizuoka 424 8610, Japan
    Front Biosci 7:d1815-24. 2002
    ..The studies described here are based on the data from traditional genetics and biochemistry, and from microarray analysis of 29 two-component systems...