Takuya Wakai

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. ncbi request reprint Induction of estrus in pubertal miniature gilts
    Takuya Wakai
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidouri Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Anim Reprod Sci 103:193-8. 2008
  2. doi request reprint Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries
    Takuya Wakai
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Cloning Stem Cells 10:249-62. 2008
  3. doi request reprint Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Cell Reprogram 12:463-74. 2010
  4. ncbi request reprint Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos
    Ken Ichi Yamanaka
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
    J Reprod Dev 53:791-800. 2007
  5. doi request reprint Effect of cytochalasins B and D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigs
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Zygote 16:153-9. 2008
  6. ncbi request reprint Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promoting factor activity during nuclear transfer
    Manabu Kawahara
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Reproduction 130:351-7. 2005
  7. ncbi request reprint Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs
    Ken Ichi Yamanaka
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
    J Reprod Dev 55:299-304. 2009
  8. doi request reprint Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Anim Sci J 81:48-57. 2010
  9. doi request reprint Molecular characteristics of horse phospholipase C zeta (PLCζ)
    Kana Sato
    Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihara 252 5201, Japan
    Anim Sci J 84:359-68. 2013
  10. doi request reprint Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes
    Junya Ito
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Japan
    Anim Sci J 81:34-41. 2010

Collaborators

Detail Information

Publications10

  1. ncbi request reprint Induction of estrus in pubertal miniature gilts
    Takuya Wakai
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidouri Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Anim Reprod Sci 103:193-8. 2008
    ..These results indicate that treatment with a low-dose combination of PMSG and hCG provides one of the simplest methods for induction of estrus and ovulation in pubertal miniature pigs...
  2. doi request reprint Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries
    Takuya Wakai
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Cloning Stem Cells 10:249-62. 2008
    ....
  3. doi request reprint Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Cell Reprogram 12:463-74. 2010
    ..Thus, anomalous oxygen consumption in porcine SCNT embryos at D5BL could be sign of limited hatchability, which may be responsible for the low TE cell number and high apoptosis incidence...
  4. ncbi request reprint Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos
    Ken Ichi Yamanaka
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
    J Reprod Dev 53:791-800. 2007
    ..9 vs. 26.1%). The present results suggest that an activation treatment of ionomycin combined with cycloheximide may avoid physical damage to microfilaments and result in improved subsequent development of miniature pig SCNT embryos...
  5. doi request reprint Effect of cytochalasins B and D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigs
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Zygote 16:153-9. 2008
    ..5 and 7.5 microg/ml CB (p < 0.05). Hence, the present findings suggest that CD is more effective than CB as the cytoskeletal inhibitor for the production of SCNT embryos in miniature pigs...
  6. ncbi request reprint Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promoting factor activity during nuclear transfer
    Manabu Kawahara
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Reproduction 130:351-7. 2005
    ..This action is most likely due to the support/increase of MPF activity throughout the process of NT...
  7. ncbi request reprint Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs
    Ken Ichi Yamanaka
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
    J Reprod Dev 55:299-304. 2009
    ....
  8. doi request reprint Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos
    Satoshi Sugimura
    Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
    Anim Sci J 81:48-57. 2010
    ..The rate of blastocyst formation was highest in the dbcAMP-treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP-treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos...
  9. doi request reprint Molecular characteristics of horse phospholipase C zeta (PLCζ)
    Kana Sato
    Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihara 252 5201, Japan
    Anim Sci J 84:359-68. 2013
    ..Taken together, our findings show for the first time that ePLCζ has highest activity of the mammalian species studied to date. Our findings will be useful for the improvement of reproductive technologies in the horse...
  10. doi request reprint Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes
    Junya Ito
    Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Japan
    Anim Sci J 81:34-41. 2010
    ..Neither inhibitor affected total expression of IP(3)R1. Altogether, our results show that IP(3)R1 undergoes dynamic phosphorylation during maturation and this might underlie the generation of oscillations at fertilization...