Research Topics
Genomes and GenesSpecies | Takuya WakaiSummaryAffiliation: Tohoku University Country: Japan Publications
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Detail Information
Publications
Induction of estrus in pubertal miniature giltsTakuya Wakai
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidouri Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
Anim Reprod Sci 103:193-8. 2008..These results indicate that treatment with a low-dose combination of PMSG and hCG provides one of the simplest methods for induction of estrus and ovulation in pubertal miniature pigs...
Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovariesTakuya Wakai
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Cloning Stem Cells 10:249-62. 2008....- Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryosKen Ichi Yamanaka
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
J Reprod Dev 53:791-800. 2007..9 vs. 26.1%). The present results suggest that an activation treatment of ionomycin combined with cycloheximide may avoid physical damage to microfilaments and result in improved subsequent development of miniature pig SCNT embryos... - Effect of cytochalasins B and D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigsSatoshi Sugimura
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
Zygote 16:153-9. 2008..5 and 7.5 microg/ml CB (p < 0.05). Hence, the present findings suggest that CD is more effective than CB as the cytoskeletal inhibitor for the production of SCNT embryos in miniature pigs... - Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryosSatoshi Sugimura
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Cell Reprogram 12:463-74. 2010..Thus, anomalous oxygen consumption in porcine SCNT embryos at D5BL could be sign of limited hatchability, which may be responsible for the low TE cell number and high apoptosis incidence... - Caffeine promotes premature chromosome condensation formation and in vitro development in porcine reconstructed embryos via a high level of maturation promoting factor activity during nuclear transferManabu Kawahara
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Reproduction 130:351-7. 2005..This action is most likely due to the support/increase of MPF activity throughout the process of NT... - Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigsKen Ichi Yamanaka
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University
J Reprod Dev 55:299-304. 2009.... - Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryosSatoshi Sugimura
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Anim Sci J 81:48-57. 2010..The rate of blastocyst formation was highest in the dbcAMP-treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP-treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos... - Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytesJunya Ito
Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Japan
Anim Sci J 81:34-41. 2010..Neither inhibitor affected total expression of IP(3)R1. Altogether, our results show that IP(3)R1 undergoes dynamic phosphorylation during maturation and this might underlie the generation of oscillations at fertilization...