Akiko Uehara

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. doi request reprint Gingipains from Porphyromonas gingivalis synergistically induce the production of proinflammatory cytokines through protease-activated receptors with Toll-like receptor and NOD1/2 ligands in human monocytic cells
    A Uehara
    Department of Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai 980 8575, Japan
    Cell Microbiol 10:1181-9. 2008
  2. doi request reprint Dual regulation of interleukin-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalis
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Med Microbiol 57:500-7. 2008
  3. ncbi request reprint Synergism between TLRs and NOD1/2 in oral epithelial cells
    A Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Dent Res 87:682-6. 2008
  4. pmc PR3-ANCA in Wegener's granulomatosis prime human mononuclear cells for enhanced activation via TLRs and NOD1/2
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    Diagn Pathol 4:23. 2009
  5. ncbi request reprint Antibodies to proteinase 3 prime human monocytic cells via protease-activated receptor-2 and NF-kappaB for Toll-like receptor- and NOD-dependent activation
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai 980 8575, Japan
    Mol Immunol 44:3552-62. 2007
  6. ncbi request reprint Arginine-specific gingipains from Porphyromonas gingivalis stimulate production of hepatocyte growth factor (scatter factor) through protease-activated receptors in human gingival fibroblasts in culture
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Immunol 175:6076-84. 2005
  7. ncbi request reprint Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Mol Immunol 44:3100-11. 2007
  8. pmc Antibodies to proteinase 3 prime human oral, lung, and kidney epithelial cells to secrete proinflammatory cytokines upon stimulation with agonists to various Toll-like receptors, NOD1, and NOD2
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai 980 8575, Japan
    Clin Vaccine Immunol 15:1060-6. 2008
  9. ncbi request reprint Neutrophil serine proteinases activate human nonepithelial cells to produce inflammatory cytokines through protease-activated receptor 2
    Akiko Uehara
    Department of Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai, Japan
    J Immunol 170:5690-6. 2003
  10. ncbi request reprint Endogenous IL-15 sustains recruitment of IL-2Rbeta and common gamma and IL-2-mediated chemokine production in normal and inflamed human gingival fibroblasts
    Akiko Ozawa
    Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Aoba ku, Sendai, Japan
    J Immunol 173:5180-8. 2004

Collaborators

Detail Information

Publications73

  1. doi request reprint Gingipains from Porphyromonas gingivalis synergistically induce the production of proinflammatory cytokines through protease-activated receptors with Toll-like receptor and NOD1/2 ligands in human monocytic cells
    A Uehara
    Department of Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai 980 8575, Japan
    Cell Microbiol 10:1181-9. 2008
    ..The host defence system against P. gingivalis may be triggered through the activation of PARs by gingipains and augmented by PAMPs from this pathogen via TLRs or NOD1/2...
  2. doi request reprint Dual regulation of interleukin-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalis
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Med Microbiol 57:500-7. 2008
    ..These results suggest that gingipains preferentially suppress IL-8, resulting in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation...
  3. ncbi request reprint Synergism between TLRs and NOD1/2 in oral epithelial cells
    A Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Dent Res 87:682-6. 2008
    ..These findings indicate that, in innate immune responses to invading microbes, a combination of signaling through TLRs and NODs leads to the synergistic activation of antibacterial responses in the oral epithelium...
  4. pmc PR3-ANCA in Wegener's granulomatosis prime human mononuclear cells for enhanced activation via TLRs and NOD1/2
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    Diagn Pathol 4:23. 2009
    ....
  5. ncbi request reprint Antibodies to proteinase 3 prime human monocytic cells via protease-activated receptor-2 and NF-kappaB for Toll-like receptor- and NOD-dependent activation
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai 980 8575, Japan
    Mol Immunol 44:3552-62. 2007
    ....
  6. ncbi request reprint Arginine-specific gingipains from Porphyromonas gingivalis stimulate production of hepatocyte growth factor (scatter factor) through protease-activated receptors in human gingival fibroblasts in culture
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Immunol 175:6076-84. 2005
    ..These results suggest that Rgps activated human gingival fibroblasts to secrete HGF in the inflamed sites and the mechanism(s) involved may actively participate in both inflammatory and reparative processes in periodontal diseases...
  7. ncbi request reprint Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Mol Immunol 44:3100-11. 2007
    ..These findings indicate that TLR and NOD in various epithelial cells are functional receptors that induce anti-bacterial responses in general without being accompanied by inflammatory responses...
  8. pmc Antibodies to proteinase 3 prime human oral, lung, and kidney epithelial cells to secrete proinflammatory cytokines upon stimulation with agonists to various Toll-like receptors, NOD1, and NOD2
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai 980 8575, Japan
    Clin Vaccine Immunol 15:1060-6. 2008
    ..The results suggest that anti-PR3 Abs (PR3 ANCA) prime human epithelial cells to produce cytokines upon stimulation with various PAMPs, and these mechanisms may be involved in severe chronic inflammation in WG...
  9. ncbi request reprint Neutrophil serine proteinases activate human nonepithelial cells to produce inflammatory cytokines through protease-activated receptor 2
    Akiko Uehara
    Department of Microbiology and Immunology, Graduate School of Dentistry, Tohoku University, Sendai, Japan
    J Immunol 170:5690-6. 2003
    ..These findings suggest that neutrophil serine proteinases have equal ability to activate human nonepithelial cells through PAR-2 to produce inflammatory cytokines and may control a number of inflammatory processes such as periodontitis...
  10. ncbi request reprint Endogenous IL-15 sustains recruitment of IL-2Rbeta and common gamma and IL-2-mediated chemokine production in normal and inflamed human gingival fibroblasts
    Akiko Ozawa
    Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry, Aoba ku, Sendai, Japan
    J Immunol 173:5180-8. 2004
    ..These results suggest that endogenous membrane-bound IL-15 sustains recruitment of IL-2Rbeta and gammac through activation of NF-kappaB in HGF...
  11. ncbi request reprint Expression of IL-2 receptor beta and gamma chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2
    Akiko Ozawa
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Leukoc Biol 74:352-9. 2003
    ....
  12. ncbi request reprint Proinflammatory cytokines induce proteinase 3 as membrane-bound and secretory forms in human oral epithelial cells and antibodies to proteinase 3 activate the cells through protease-activated receptor-2
    Akiko Uehara
    Department of Oral Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Immunol 173:4179-89. 2004
    ....
  13. ncbi request reprint Meso-diaminopimelic acid and meso-lanthionine, amino acids specific to bacterial peptidoglycans, activate human epithelial cells through NOD1
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan
    J Immunol 177:1796-804. 2006
    ....
  14. ncbi request reprint Innate immune responses in oral mucosa
    Shunji Sugawara
    Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai, Japan
    J Endotoxin Res 8:465-8. 2002
    ..In addition, saliva contains abundant bio-active CD14 from salivary glands in a soluble form, although LPS-binding protein was below detectable levels, suggesting that saliva CD14 is important for the maintenance of oral health...
  15. ncbi request reprint Activation of human oral epithelial cells by neutrophil proteinase 3 through protease-activated receptor-2
    Akiko Uehara
    Department of Microbiology and Immunology, School of Dentistry, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Immunol 169:4594-603. 2002
    ..These findings suggest that neutrophil PR3 activates oral epithelial cells through G protein-coupled PAR-2 and actively participates in the process of inflammation such as periodontitis...
  16. ncbi request reprint Molecular cloning and functional characterization of porcine nucleotide-binding oligomerization domain-1 (NOD1) recognizing minimum agonists, meso-diaminopimelic acid and meso-lanthionine
    Masanori Tohno
    Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
    Mol Immunol 45:1807-17. 2008
    ..Our results should help clarify how the intestinal immune system is modulated by low-molecular weight peptidoglycan fragments through NOD1...
  17. ncbi request reprint [Oral bacteria and periodontitis, with special reference to innate immune system in oral mucosa]
    Akiko Uehara
    Tohoku University Graduate School of Dentistry, Department of Microbiology and Immunology
    Clin Calcium 17:173-8. 2007
    ....
  18. pmc Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production
    Hiroyuki Tada
    Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980 8575, Japan
    Infect Immun 70:3304-7. 2002
    ....
  19. doi request reprint A polymer-type water-soluble peptidoglycan exhibited both Toll-like receptor 2- and NOD2-agonistic activities, resulting in synergistic activation of human monocytic cells
    Mizuho Natsuka
    Department of Microbiology and Immunology, Tohoku University School of Dentistry, Aoba ku, Sendai, Japan
    Innate Immun 14:298-308. 2008
    ..These findings strongly suggested that a polymer-type water-soluble PGN fragment, SEPS, exhibits both TLR2-and NOD2-agonistic activities, which induced the synergistic activation of human monocytic cells...
  20. pmc Constitutive expression of a bacterial pattern recognition receptor, CD14, in human salivary glands and secretion as a soluble form in saliva
    Akiko Uehara
    Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980 8575, Japan
    Clin Diagn Lab Immunol 10:286-92. 2003
    ..These results suggested that saliva CD14 is important for the maintenance of oral health and possibly intestinal homeostasis...
  21. pmc Mouse and human cell activation by N-dodecanoyl-DL-homoserine lactone, a Chromobacterium violaceum autoinducer
    Kazunori Gomi
    Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Infect Immun 74:7029-31. 2006
    ....
  22. ncbi request reprint Exploring the Sn binding pockets in gingipains by newly developed inhibitors: structure-based design, chemistry, and activity
    Arkadiusz Białas
    Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    J Med Chem 49:1744-53. 2006
    ..The detailed analysis of Sn binding pockets revealed the molecular basis of inhibitory affinity and provided insight into the structure-activity relationship...
  23. pmc Gingipains from Porphyromonas gingivalis W83 synergistically disrupt endothelial cell adhesion and can induce caspase-independent apoptosis
    Shaun M Sheets
    Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Infect Immun 74:5667-78. 2006
    ..Degradation of integrin beta1 was inhibited only in the presence of z-VAD-FMK. These results further clarify the role P. gingivalis plays in tissue destruction occurring in the periodontal pocket...
  24. ncbi request reprint The C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities
    Maryta Sztukowska
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Mol Microbiol 54:1393-408. 2004
    ..gingivalis outer membrane. Moreover, our results indicate that the immunoglobulin-like subdomain is indispensable for proper folding and expression of the gingipains...
  25. ncbi request reprint Selective inhibition of Porphyromonas gingivalis growth by a factor Xa inhibitor, DX-9065a
    Kenji Matsushita
    Department of Oral Disease Research, National Institute for Longevity Science, Aichi, Japan
    J Periodontal Res 41:171-6. 2006
    ..Porphyromonas gingivalis is a causative bacterium of adult periodontitis. However, there is no drug specific for P. gingivalis and for its virulence factor...
  26. pmc Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-Negative bacteria?
    Ky Anh Nguyen
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Science Bldg, Rm A322, Athens, GA 30602, USA
    J Bacteriol 189:833-43. 2007
    ..This result may have a wider implication in a novel secretory pathway in distinct members of the Cytophaga-Flavobacterium-Bacteroidetes phylum...
  27. ncbi request reprint Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the haem-containing pigment from oxyhaemoglobin
    John W Smalley
    Department of Clinical Dental Sciences, The University of Liverpool, Liverpool, UK
    Arch Biochem Biophys 465:44-9. 2007
    ..W. Smalley, M.F. Thomas, A.J. Birss, R. Withnall, J. Silver, Biochem. J. 379 (2004) 833-840.] of the requirement for a combination of both R- and K-gingipains for pigment production from oxyhaemoglobin by P. gingivalis...
  28. ncbi request reprint Friendly fire against neutrophils: proteolytic enzymes confuse the recognition of apoptotic cells by macrophages
    Krzysztof Guzik
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 90:405-15. 2008
    ....
  29. pmc Hydrolysis of epithelial junctional proteins by Porphyromonas gingivalis gingipains
    Jannet Katz
    Department of Oral Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Infect Immun 70:2512-8. 2002
    ..gingivalis...
  30. doi request reprint Purification and characterization of gingipains
    Jan Potempa
    Jagiellonian University, Krakow, Poland
    Curr Protoc Protein Sci . 2007
    ..Obtained from these procedures, the gingipains are stable and can be stored at -80 degrees C for years without loss of activity...
  31. pmc Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis
    John W Smalley
    Unit of Plaque Related Diseases, School of Dental Science, The University of Liverpool, Liverpool L69 3GN, UK
    Biol Chem 389:1235-8. 2008
    ..Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack...
  32. pmc Binding of complement inhibitor C4b-binding protein contributes to serum resistance of Porphyromonas gingivalis
    Michal Potempa
    Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Lund University, Malmo, Sweden
    J Immunol 181:5537-44. 2008
    ....
  33. ncbi request reprint Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system
    Katarzyna Popadiak
    Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Malmo, Sweden
    J Immunol 178:7242-50. 2007
    ..At later stages of infection the concentration of proteases is high enough to destroy complement factors and thus render the bacteria resistant to the bactericidal activity of complement...
  34. ncbi request reprint Neuropeptide release from dental pulp cells by RgpB via proteinase-activated receptor-2 signaling
    Salunya Tancharoen
    Department of Restorative Dentistry and Endodontology, Laboratory of Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Science, Japan
    J Immunol 174:5796-804. 2005
    ..This new RgpB activity suggests a possible link between periodontitis and pulp inflammation, which may be modulated by neuropeptides released in the lesion...
  35. pmc Gingipains from Porphyromonas gingivalis W83 induce cell adhesion molecule cleavage and apoptosis in endothelial cells
    Shaun M Sheets
    Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Infect Immun 73:1543-52. 2005
    ..Taken together, these results indicate that gingipains from P. gingivalis can alter cell adhesion molecules and induce endothelial cell death, which could have implications for the pathogenicity of this organism...
  36. ncbi request reprint Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Curr Protein Pept Sci 4:397-407. 2003
    ..This hypothetical scenario is a reasonable explanation for the occurrence of many forms of gingipains...
  37. ncbi request reprint Coordinate expression of the Porphyromonas gingivalis lysine-specific gingipain proteinase, Kgp, arginine-specific gingipain proteinase, RgpA, and the heme/hemoglobin receptor, HmuR
    Xinyan Liu
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Biol Chem 385:1049-57. 2004
    ..Collectively, these results indicate that kgp , rgpA and hmuR gene transcription is coordinately regulated and may facilitate greater efficiency of heme utilization in P. gingivalis ...
  38. ncbi request reprint Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes
    Nafisa Ally
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Biochemistry 42:11693-700. 2003
    ....
  39. ncbi request reprint Sequential autolytic processing activates the zymogen of Arg-gingipain
    Jowita Mikolajczyk
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 278:10458-64. 2003
    ..Each step in activation requires the previous step, and an affinity probe reveals that incremental activity enhancements are achieved in a stepwise manner...
  40. ncbi request reprint The biphasic virulence activities of gingipains: activation and inactivation of host proteins
    Takahisa Imamura
    Division of Molecular Pathology, Kumamoto University Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Curr Protein Pept Sci 4:443-50. 2003
    ..gingivalis. Gingipains are potent virulence factors of P. gingivalis, and in many regards their pathogenic activities constitute new mechanisms of bacterial virulence...
  41. pmc Inactivation of membrane tumor necrosis factor alpha by gingipains from Porphyromonas gingivalis
    Renata Mezyk-Kopec
    Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Cracow, Poland
    Infect Immun 73:1506-14. 2005
    ..Thus, gingipains are able not only to cleave soluble TNF-alpha but also to destroy the membrane form of the cytokine, which may additionally dysregulate the cytokine network...
  42. ncbi request reprint Proteolysis of interleukin-6 receptor (IL-6R) by Porphyromonas gingivalis cysteine proteinases (gingipains) inhibits interleukin-6-mediated cell activation
    Aneta Oleksy
    Institute of Molecular Biology, Jagellonian University, Krakow, Poland
    Microb Pathog 32:173-81. 2002
    ..gingivalis the inflammatory reactions dependent on IL-6 could be severely hindered contributing to both tissue damage and periodontopathogen survival...
  43. ncbi request reprint Ribonucleotide reductase class III, an essential enzyme for the anaerobic growth of Staphylococcus aureus, is a virulence determinant in septic arthritis
    Ebru Kirdis
    Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10A, S 413 46 Goteborg, Sweden
    Microb Pathog 43:179-88. 2007
    ..Together these results indicate that RNR class III is an important virulence factor for the establishment of septic arthritis...
  44. pmc Porphyromonas gingivalis HmuY and HmuR: further characterization of a novel mechanism of heme utilization
    Teresa Olczak
    Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
    Arch Microbiol 189:197-210. 2008
    ....
  45. ncbi request reprint A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biochemistry 42:13449-56. 2003
    ....
  46. ncbi request reprint In vivo sortase A and clumping factor A mRNA expression during Staphylococcus aureus infection
    Elisabet Josefsson
    Department of Rheumatology and Inflammation Research, Goteborg University, Guldhedsgatan 10, Goteborg, Sweden
    Microb Pathog 44:103-10. 2008
    ..Possibly enters the majority of bacteria a metabolically dormant steady state at high bacterial loads...
  47. pmc A new autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A
    Noemí Mallorquí-Fernández
    Departament de Biologia Estructural, Institut de Biologia Molecular de Barcelona, Consejo Superior de Investigaciones Cientificas, C Jordi Girona 18 26, Barcelona, Spain
    J Biol Chem 283:2871-82. 2008
    ..These findings can be extrapolated to related potentially pathogenic cysteine proteases such as Streprococcus pyogenes SpeB and Porphyromonas gingivalis periodontain...
  48. pmc A potential new pathway for Staphylococcus aureus dissemination: the silent survival of S. aureus phagocytosed by human monocyte-derived macrophages
    Malgorzata Kubica
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 3:e1409. 2008
    ..S. aureus persists inside macrophages for several days without affecting the viability of these mobile cells which may serve as vehicles for the dissemination of infection...
  49. pmc Staphostatins resemble lipocalins, not cystatins in fold
    Malgorzata Rzychon
    International Institute of Molecular and Cell Biology, 02109 Warsaw, Poland
    Protein Sci 12:2252-6. 2003
    ..Unexpectedly for a cysteine protease inhibitor, staphostatin B is not significantly similar to cystatins...
  50. ncbi request reprint Staphostatins: an expanding new group of proteinase inhibitors with a unique specificity for the regulation of staphopains, Staphylococcus spp. cysteine proteinases
    Malgorzata Rzychon
    Department of Analytical Biochemistry, Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mol Microbiol 49:1051-66. 2003
    ....
  51. ncbi request reprint The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    J Biol Chem 278:40959-66. 2003
    ..Mutations in this residue lead to a loss of affinity of the inhibitor for protease and convert the inhibitor into a substrate...
  52. ncbi request reprint Inhibition of arginine gingipains (RgpB and HRgpA) with benzamidine inhibitors: zinc increases inhibitory potency
    Joel A Krauser
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30093 0400, USA
    Biol Chem 383:1193-8. 2002
    ..In summary, we have discovered a new series of effective inhibitors for the gingipains and found a novel way to increase inhibitor potency with the HRgpA and RgpB gingipains using zinc...
  53. ncbi request reprint Proteinase-mediated release of epithelial cell-associated CD44. Extracellular CD44 complexes with components of cellular matrices
    Joanna Cichy
    Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
    J Biol Chem 277:44440-7. 2002
    ....
  54. ncbi request reprint Poison-antidote systems in bacteria: the co-evolution of functional counterparts
    Jan Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Cell Mol Biol (Noisy-le-grand) 52:18-22. 2006
    ....
  55. ncbi request reprint On the transcriptional regulation of methicillin resistance: MecI repressor in complex with its operator
    Raquel García-Castellanos
    Institut de Biologia Molecular de Barcelona, CID CSIC C Jordi Girona, 18 26, 08034 Barcelona, Spain
    J Biol Chem 279:17888-96. 2004
    ..The structure of this first molecular determinant of methicillin resistance in complex with its target DNA provides insights into its regulatory mechanism and paves the way for new antimicrobial strategies against MRSA...
  56. ncbi request reprint Aza-peptide Michael acceptors: a new class of inhibitors specific for caspases and other clan CD cysteine proteases
    Ozlem Dogan Ekici
    School of Chemistry and Biochemistry and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    J Med Chem 47:1889-92. 2004
    ..Aza-Lys and aza-Orn derivatives were potent inhibitors of gingipain K and clostripain. Aza-peptide Michael acceptors showed no cross reactivity toward papain, cathepsin B, and calpain...
  57. pmc Induction of vascular leakage through release of bradykinin and a novel kinin by cysteine proteinases from Staphylococcus aureus
    Takahisa Imamura
    Division of Molecular Pathology, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    J Exp Med 201:1669-76. 2005
    ..aureus virulence and bacterial shock. Therefore, staphopain-specific inhibitors and kinin-receptor antagonists could be used to treat this disease...
  58. ncbi request reprint Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mol Microbiol 57:605-10. 2005
    ..This apparently represents a novel system that bacteria use to control the intracellular activity of their proteases...
  59. ncbi request reprint Effects of elastase and cathepsin G on the levels of membrane and soluble TNFalpha
    Renata Mezyk-Kopec
    Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 386:801-11. 2005
    ..Taken together, the ability of NE and CG to modulate levels of membrane and soluble forms of TNFalpha may contribute to the proinflammatory activity of neutrophils...
  60. pmc Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotype
    Lindsey N Shaw
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Bldg, Athens, GA 30602, USA
    J Bacteriol 187:1751-62. 2005
    ..Seemingly, some of these proteins may play a role in protein secretion; hence, their proteolytic inactivation by SspB has pleiotropic effects on the SspC-deficient mutant...
  61. ncbi request reprint A comparison of staphostatin B with standard mechanism serine protease inhibitors
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    J Biol Chem 280:14669-74. 2005
    ....
  62. ncbi request reprint Genetic characterization of staphopain genes in Staphylococcus aureus
    Ewa Golonka
    Department of Microbiology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biol Chem 385:1059-67. 2004
    ....
  63. pmc Roles of the host oxidative immune response and bacterial antioxidant rubrerythrin during Porphyromonas gingivalis infection
    Piotr Mydel
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS Pathog 2:e76. 2006
    ..gingivalis by exacerbating local and systemic inflammation, thereby contributing to the morbidity and mortality associated with infection...
  64. ncbi request reprint Interaction of a novel form of Pseudomonas aeruginosa alkaline protease (aeruginolysin) with interleukin-6 and interleukin-8
    Nancy R Matheson
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    Biol Chem 387:911-5. 2006
    ..Interestingly, aeruginolysin bearing two additional residues at the N-terminus (Leu-Lys-aeruginolysin) in the absence of calcium degraded both IL-6 and IL-8-72 far more efficiently than the shorter form of the enzyme...
  65. pmc Inhibition of tumor necrosis factor-alpha-induced RANTES secretion by alkaline protease in A549 cells
    Thomas M Krunkosky
    Department of Anatomy and Radiology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30606, USA
    Am J Respir Cell Mol Biol 33:483-9. 2005
    ..These results demonstrate that AP stimulates shedding of cell-surface TNFR1, resulting in an increase in sTNFR1. Consequently, these events decrease the cells' ability to stimulate RANTES gene expression and secretion through TNFR1...
  66. ncbi request reprint Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    Biochemistry 43:14306-15. 2004
    ..In a modeled complex of prostaphopain B with staphostatin B, clashes occur both inside and outside the active site cleft, but involve mostly poorly ordered regions of the protein that may be mobile...
  67. ncbi request reprint Bacterial peptidases
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, PL 30 387 Krakow, Poland
    Contrib Microbiol 12:132-80. 2005
  68. ncbi request reprint The staphostatin family of cysteine protease inhibitors in the genus Staphylococcus as an example of parallel evolution of protease and inhibitor specificity
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 388:227-35. 2007
    ..The tight coevolution is likely the result of the known deleterious effects of uncontrolled staphopain action...
  69. ncbi request reprint Characterisation of a highly specific, endogenous inhibitor of cysteine protease from Staphylococcus epidermidis, a new member of the staphostatin family
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biol Chem 385:543-6. 2004
    ..The difference in the susceptibility of individual inhibitors to proteolytic cleavage at the reactive site suggests subtle variations in the mechanism of interaction with cysteine proteases...
  70. ncbi request reprint Staphylococcus aureus-derived staphopain B, a potent cysteine protease activator of plasma chemerin
    Paulina Kulig
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Ulica Gronostajowa 7, 30 387 Krakow, Poland
    J Immunol 178:3713-20. 2007
    ..SspB may help direct the recruitment of specialized host cells, including immunoregulatory pDC and/or macrophages, contributing to the ability of S. aureus to elicit and maintain a chronic inflammatory state...
  71. ncbi request reprint Down-regulation of human extracellular cysteine protease inhibitors by the secreted staphylococcal cysteine proteases, staphopain A and B
    Bjarne Vincents
    Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, University Hospital, S 221 85 Lund, Sweden
    Biol Chem 388:437-46. 2007
    ..Results presented for the specificity of staphopains when interacting with cystatins as natural protein substrates could aid in the development of therapeutic agents directed toward these proteolytic virulence factors...
  72. ncbi request reprint Growth phase-dependent production of a cell wall-associated elastinolytic cysteine proteinase by Staphylococcus epidermidis
    Aneta Oleksy
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 385:525-35. 2004
    ..This enzyme, with elastinolytic properties, as well as the ability to cleave alpha1PI, fibrinogen and fibronectin, may possibly contribute to the invasiveness and pathogenic potential of S. epidermidis...
  73. ncbi request reprint Role of rubrerythrin in the oxidative stress response of Porphyromonas gingivalis
    Maryta Sztukowska
    Institute of Molecular Biology, Jagiellonian University, 31 120 Krakow, Poland
    Mol Microbiol 44:479-88. 2002
    ..Porphyromonas gingivalis contains a superoxide dismutase but lacks catalase and haem peroxidases. We therefore suggest that rubrerythrin provides oxidative stress protection via catalytic reduction of intracellular hydrogen peroxide...