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Genomes and Genes | S TadaSummaryAffiliation: Tohoku University Country: Japan Publications
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Publications
Cdt1 and geminin: role during cell cycle progression and DNA damage in higher eukaryotesShusuke Tada
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
Front Biosci 12:1629-41. 2007..Furthermore, Cdt1 is also proteolyzed in G1 phase in response to DNA damage, presumably providing a new checkpoint control...
Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cellsS Tada
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
Nucleic Acids Res 29:3835-40. 2001..The cDNA encoding DNA helicase B from tsFT848 was sequenced and a mutation was found between DNA/RNA helicase motifs IV and V...
Insight into initiator-DNA interactions: a lesson from the archaeal ORCShusuke Tada
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
Bioessays 30:208-11. 2008..Since the archaeal replication system is postulated as a simplified version of the one in eukaryotes, by analogy, these works provide insights into the functions of the eukaryotic initiator proteins...- BLM is an early responder to DNA double-strand breaksParimal Karmakar
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochem Biophys Res Commun 348:62-9. 2006..Thus, HRDC domain in DNA helicases is a common early responder to DNA double-strand breaks, enabling BLM and WRN to be involved in DNA repair... - Functional relation among RecQ family helicases RecQL1, RecQL5, and BLM in cell growth and sister chromatid exchange formationWensheng Wang
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
Mol Cell Biol 23:3527-35. 2003..Surprisingly, RECQL5(-/-)/BLM(-/-) cells showed a higher frequency of SCE than BLM(-/-) cells, indicating that RecQL5 suppresses SCE under the BLM function-impaired condition... - Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalphaMakoto Otsuki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, Japan
Biochim Biophys Acta 1782:75-81. 2008..However, recql5/blm cells showed higher frequency of SCE than blm cells, whereas the RECQL1 gene disruption had no effect on SCE in blm cells and even in recql5/blm cells... - Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stabilityTomoko Hayashi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan
Genes Genet Syst 83:95-100. 2008..This result suggests that WRNIP1 and BLM function independently to repair DNA or induce tolerance to the lesions induced by CPT... - The absence of a functional relationship between ATM and BLM, the components of BASC, in DT40 cellsWensheng Wang
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan
Biochim Biophys Acta 1688:137-44. 2004..Disrupting the function of ATM reduced the targeted integration frequency in BLM(-/-) DT40 cells. However, a defect in ATM only slightly reduced the increased sister chromatid exchanges (SCEs) in BLM(-/-) DT40 cells... - Cloning of two isoforms of mouse DNA helicase Q1/RecQL cDNA; alpha form is expressed ubiquitously and beta form specifically in the testisW S Wang
Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochim Biophys Acta 1443:198-202. 1998.... - WRN functions in a RAD18-dependent damage avoidance pathwayYu Peng Dong
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
Biol Pharm Bull 30:1080-3. 2007..Moreover, the cisplatin sensitivity of RAD18(-/-) cells was slightly suppressed by disruption of WRN. These data suggest that WRN functions in a pathway involving RAD18 under damage-inducing conditions... - Bloom helicase and DNA topoisomerase IIIalpha are involved in the dissolution of sister chromatidsMasayuki Seki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Mol Cell Biol 26:6299-307. 2006..Taken together with the biochemical properties of BLM and Top3alpha, these data indicate that BLM and Top3alpha execute the dissolution of sister chromatids... - Physical and functional interaction between WRNIP1 and RAD18Akari Yoshimura
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
Genes Genet Syst 84:171-8. 2009..In contrast, RAD18 enhances the binding of WRNIP1 to these DNAs, suggesting that WRNIP1 targets DNA bound by RAD18... - Analyses of the interaction of WRNIP1 with Werner syndrome protein (WRN) in vitro and in the cellYoh ichi Kawabe
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Sendai 980 8578, Japan
DNA Repair (Amst) 5:816-28. 2006..Mutations in the Walker A motifs of the two proteins revealed that WRNIP1, but not WRN, must bind ATP before an efficient interaction can occur... - Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extractsTakashi Tsuyama
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980 8578, Japan
Mol Biol Cell 20:937-47. 2009..We propose a novel surveillance mechanism in which Cdt1 blocks nascent chain elongation after detecting illegitimate activation of the licensing system... - RecQ family helicases in genome stability: lessons from gene disruption studies in DT40 cellsMasayuki Seki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
Cell Cycle 7:2472-8. 2008..Here, we review the functions of eukaryotic RecQ helicases, focusing primarily on BLM in the maintenance of genome stability through various pathways of nucleic acid metabolism and with special reference to DNA replication... - Functional interactions between BLM and XRCC3 in the cellMakoto Otsuki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980 8578, Japan
J Cell Biol 179:53-63. 2007..Moreover, disruption of XRCC3 suppresses MMS and UV sensitivity and the MMS- and UV-induced chromosomal aberrations of blm cells, indicating that BLM acts downstream of XRCC3... - Function of recQ family helicase in genome stabilityMasayuki Seki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
Subcell Biochem 40:49-73. 2006..Here, we briefly review general features of RecQ helicases and describe their functions as revealed by analysis of DT40 cells... - Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complexLena R Kundu
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
Nucleic Acids Res 38:5409-18. 2010.... - Focus-formation of replication protein A, activation of checkpoint system and DNA repair synthesis induced by DNA double-strand breaks in Xenopus egg extractTakayuki Kobayashi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8578, Japan
J Cell Sci 115:3159-69. 2002..The biotin-dUTP signal co-localized with replication protein A foci and was not significantly suppressed or stimulated by the addition of caffeine... - A novel Rad18 function involved in protection of the vertebrate genome after exposure to camptothecinAkari Yoshimura
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Sendai 980 8578, Japan
DNA Repair (Amst) 5:1307-16. 2006..These data suggest a previously unanticipated role for Rad18 in dealing with replication forks upon encountering DNA lesions induced by CPT... - Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extractsTakashi Tsuyama
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University Aoba-ku, Sendai, Miyagi 980-8578, Japan
Nucleic Acids Res 33:765-75. 2005..These results provide evidence supporting that Cdc6 and Cdt1 must bind to chromatin in a strict order for DNA licensing to occur... - [DNA helicases and progeroid syndromes]Shusuke Tada
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8578, Japan
Seikagaku 78:221-9. 2006 - Dpb11, the budding yeast homolog of TopBP1, functions with the checkpoint clamp in recombination repairHideaki Ogiwara
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Nucleic Acids Res 34:3389-98. 2006..These results indicate that, besides the involvement in the replication and checkpoint, Dpb11 functions with Ddc1 in the recombination repair process itself... - Ctf18 is required for homologous recombination-mediated double-strand break repairHideaki Ogiwara
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Nucleic Acids Res 35:4989-5000. 2007..These results suggest that Ctf18 is involved in damage-induced homologous recombination... - Functional relationships between Rad18 and WRNIP1 in vertebrate cellsAkari Yoshimura
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
Biol Pharm Bull 29:2192-6. 2006..Unexpectedly, the severe CPT sensitivity of RAD18-/- cells is slightly suppressed by disruption of the WRNIP1 gene... - Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replicationTakashi Ohuchi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
DNA Repair (Amst) 8:690-6. 2009..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed... - Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cellsAyako Ui
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochem Biophys Res Commun 356:1031-7. 2007..Lastly, a putative role of Mms1 in the elevation of uSCR and a possible mechanism by which uSCR is elevated as a result of defective Sgs1 and Rad51 are discussed... - Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaksTakashi Tsuyama
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
Biochem Biophys Res Commun 351:935-9. 2006..These findings suggest that the Smc5/6 complex is regulated during the cell cycle, presumably in anticipation of DNA damage that may arise during replication... - Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2Shunya Takada
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochem Biophys Res Commun 379:233-8. 2009..In contrast to results obtained with mouse and DrosophilaSod2 mutants, we found no indication of an increase in DNA lesions due to depletion of SOD2... - KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formationTakuya Abe
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Cell Signal 20:1978-85. 2008..These results suggest that the proteins involved in the early stage of NHEJ pathway including Artemis but not the downstream factors decide the cell fate by selecting apoptosis or DNA repair according to the degree of DNA damage... - Rad52 sumoylation and its involvement in the efficient induction of homologous recombinationTakashi Ohuchi
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
DNA Repair (Amst) 7:879-89. 2008.... - The INO80 complex is required for damage-induced recombinationSatoshi Kawashima
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochem Biophys Res Commun 355:835-41. 2007..Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologous recombination upon DNA damages... - Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cellsYuji Tomizawa
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan
Genes Genet Syst 82:157-60. 2007..However, UV-induced recombination between homologous chromosomes is intact in both rad50 and smc6-56 mutant cells... - A novel role for Rad17 in homologous recombinationKatsuaki Nishino
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
Genes Genet Syst 83:427-31. 2008..Thus, in addition to its role in the replication checkpoint, Rad17 appears to play a role in homologous recombination... - WRN counteracts the NHEJ pathway upon camptothecin exposureMakoto Otsuki
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
Biochem Biophys Res Commun 355:477-82. 2007..The implications for the function of WRN in the non-homologous end-joining pathway of DNA repair involving Ku70 and DNA-PKcs, which may be the cause of lethality in the presence of CPT, will be discussed... - Structural basis for inhibition of the replication licensing factor Cdt1 by gemininChangwook Lee
National Creative Research Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo ja dong, San31, Pohang, KyungBook, South Korea
Nature 430:913-7. 2004.... - Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line developmentKen ichiro Yanagi
Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Wako, Saitama, Japan
J Biol Chem 280:19689-94. 2005..We conclude that the Cdt1-geminin system is conserved throughout metazoans and that geminin has evolved in these taxa to regulate proliferation and differentiation by directly interacting with Cdt1 and homeobox proteins... - Geminin becomes activated as an inhibitor of Cdt1/RLF-B following nuclear importBen Hodgson
Cancer Research UK Chromosome Replication, Research Group, Wellcome Trust Biocentre, University of Dundee, Dow Street, DD1 5EH, Dundee, United Kingdom
Curr Biol 12:678-83. 2002..Since the initiation of replication at licensed origins depends on nuclear assembly, our results suggest an elegant and novel mechanism for preventing rereplication of DNA in a single cell cycle...