S Tada

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. pmc Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts
    Takashi Tsuyama
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University Aoba ku, Sendai, Miyagi 980 8578, Japan
    Nucleic Acids Res 33:765-75. 2005
  2. doi request reprint Insight into initiator-DNA interactions: a lesson from the archaeal ORC
    Shusuke Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Bioessays 30:208-11. 2008
  3. pmc Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells
    S Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Nucleic Acids Res 29:3835-40. 2001
  4. ncbi request reprint Cdt1 and geminin: role during cell cycle progression and DNA damage in higher eukaryotes
    Shusuke Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Front Biosci 12:1629-41. 2007
  5. ncbi request reprint BLM is an early responder to DNA double-strand breaks
    Parimal Karmakar
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 348:62-9. 2006
  6. pmc Functional relation among RecQ family helicases RecQL1, RecQL5, and BLM in cell growth and sister chromatid exchange formation
    Wensheng Wang
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Cell Biol 23:3527-35. 2003
  7. ncbi request reprint The absence of a functional relationship between ATM and BLM, the components of BASC, in DT40 cells
    Wensheng Wang
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Biochim Biophys Acta 1688:137-44. 2004
  8. ncbi request reprint Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha
    Makoto Otsuki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, Japan
    Biochim Biophys Acta 1782:75-81. 2008
  9. ncbi request reprint Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability
    Tomoko Hayashi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan
    Genes Genet Syst 83:95-100. 2008
  10. ncbi request reprint Cloning of two isoforms of mouse DNA helicase Q1/RecQL cDNA; alpha form is expressed ubiquitously and beta form specifically in the testis
    W S Wang
    Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochim Biophys Acta 1443:198-202. 1998

Collaborators

Detail Information

Publications38

  1. pmc Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts
    Takashi Tsuyama
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University Aoba ku, Sendai, Miyagi 980 8578, Japan
    Nucleic Acids Res 33:765-75. 2005
    ..These results provide evidence supporting that Cdc6 and Cdt1 must bind to chromatin in a strict order for DNA licensing to occur...
  2. doi request reprint Insight into initiator-DNA interactions: a lesson from the archaeal ORC
    Shusuke Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Bioessays 30:208-11. 2008
    ..Since the archaeal replication system is postulated as a simplified version of the one in eukaryotes, by analogy, these works provide insights into the functions of the eukaryotic initiator proteins...
  3. pmc Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells
    S Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Nucleic Acids Res 29:3835-40. 2001
    ..The cDNA encoding DNA helicase B from tsFT848 was sequenced and a mutation was found between DNA/RNA helicase motifs IV and V...
  4. ncbi request reprint Cdt1 and geminin: role during cell cycle progression and DNA damage in higher eukaryotes
    Shusuke Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Front Biosci 12:1629-41. 2007
    ..Furthermore, Cdt1 is also proteolyzed in G1 phase in response to DNA damage, presumably providing a new checkpoint control...
  5. ncbi request reprint BLM is an early responder to DNA double-strand breaks
    Parimal Karmakar
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 348:62-9. 2006
    ..Thus, HRDC domain in DNA helicases is a common early responder to DNA double-strand breaks, enabling BLM and WRN to be involved in DNA repair...
  6. pmc Functional relation among RecQ family helicases RecQL1, RecQL5, and BLM in cell growth and sister chromatid exchange formation
    Wensheng Wang
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Cell Biol 23:3527-35. 2003
    ..Surprisingly, RECQL5(-/-)/BLM(-/-) cells showed a higher frequency of SCE than BLM(-/-) cells, indicating that RecQL5 suppresses SCE under the BLM function-impaired condition...
  7. ncbi request reprint The absence of a functional relationship between ATM and BLM, the components of BASC, in DT40 cells
    Wensheng Wang
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Biochim Biophys Acta 1688:137-44. 2004
    ..Disrupting the function of ATM reduced the targeted integration frequency in BLM(-/-) DT40 cells. However, a defect in ATM only slightly reduced the increased sister chromatid exchanges (SCEs) in BLM(-/-) DT40 cells...
  8. ncbi request reprint Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha
    Makoto Otsuki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, Japan
    Biochim Biophys Acta 1782:75-81. 2008
    ..However, recql5/blm cells showed higher frequency of SCE than blm cells, whereas the RECQL1 gene disruption had no effect on SCE in blm cells and even in recql5/blm cells...
  9. ncbi request reprint Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability
    Tomoko Hayashi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan
    Genes Genet Syst 83:95-100. 2008
    ..This result suggests that WRNIP1 and BLM function independently to repair DNA or induce tolerance to the lesions induced by CPT...
  10. ncbi request reprint Cloning of two isoforms of mouse DNA helicase Q1/RecQL cDNA; alpha form is expressed ubiquitously and beta form specifically in the testis
    W S Wang
    Department of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochim Biophys Acta 1443:198-202. 1998
    ....
  11. ncbi request reprint Analyses of the interaction of WRNIP1 with Werner syndrome protein (WRN) in vitro and in the cell
    Yoh ichi Kawabe
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Sendai 980 8578, Japan
    DNA Repair (Amst) 5:816-28. 2006
    ..Mutations in the Walker A motifs of the two proteins revealed that WRNIP1, but not WRN, must bind ATP before an efficient interaction can occur...
  12. ncbi request reprint RecQ family helicases in genome stability: lessons from gene disruption studies in DT40 cells
    Masayuki Seki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Cell Cycle 7:2472-8. 2008
    ..Here, we review the functions of eukaryotic RecQ helicases, focusing primarily on BLM in the maintenance of genome stability through various pathways of nucleic acid metabolism and with special reference to DNA replication...
  13. pmc Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts
    Takashi Tsuyama
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, 980 8578, Japan
    Mol Biol Cell 20:937-47. 2009
    ..We propose a novel surveillance mechanism in which Cdt1 blocks nascent chain elongation after detecting illegitimate activation of the licensing system...
  14. pmc Bloom helicase and DNA topoisomerase IIIalpha are involved in the dissolution of sister chromatids
    Masayuki Seki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Cell Biol 26:6299-307. 2006
    ..Taken together with the biochemical properties of BLM and Top3alpha, these data indicate that BLM and Top3alpha execute the dissolution of sister chromatids...
  15. ncbi request reprint WRN functions in a RAD18-dependent damage avoidance pathway
    Yu Peng Dong
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Biol Pharm Bull 30:1080-3. 2007
    ..Moreover, the cisplatin sensitivity of RAD18(-/-) cells was slightly suppressed by disruption of WRN. These data suggest that WRN functions in a pathway involving RAD18 under damage-inducing conditions...
  16. ncbi request reprint Physical and functional interaction between WRNIP1 and RAD18
    Akari Yoshimura
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Genes Genet Syst 84:171-8. 2009
    ..In contrast, RAD18 enhances the binding of WRNIP1 to these DNAs, suggesting that WRNIP1 targets DNA bound by RAD18...
  17. pmc Functional interactions between BLM and XRCC3 in the cell
    Makoto Otsuki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Science, Tohoku University, Sendai 980 8578, Japan
    J Cell Biol 179:53-63. 2007
    ..Moreover, disruption of XRCC3 suppresses MMS and UV sensitivity and the MMS- and UV-induced chromosomal aberrations of blm cells, indicating that BLM acts downstream of XRCC3...
  18. ncbi request reprint Function of recQ family helicase in genome stability
    Masayuki Seki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Subcell Biochem 40:49-73. 2006
    ..Here, we briefly review general features of RecQ helicases and describe their functions as revealed by analysis of DT40 cells...
  19. pmc Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex
    Lena R Kundu
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Nucleic Acids Res 38:5409-18. 2010
    ....
  20. ncbi request reprint Focus-formation of replication protein A, activation of checkpoint system and DNA repair synthesis induced by DNA double-strand breaks in Xenopus egg extract
    Takayuki Kobayashi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Cell Sci 115:3159-69. 2002
    ..The biotin-dUTP signal co-localized with replication protein A foci and was not significantly suppressed or stimulated by the addition of caffeine...
  21. ncbi request reprint A novel Rad18 function involved in protection of the vertebrate genome after exposure to camptothecin
    Akari Yoshimura
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Sendai 980 8578, Japan
    DNA Repair (Amst) 5:1307-16. 2006
    ..These data suggest a previously unanticipated role for Rad18 in dealing with replication forks upon encountering DNA lesions induced by CPT...
  22. pmc Dpb11, the budding yeast homolog of TopBP1, functions with the checkpoint clamp in recombination repair
    Hideaki Ogiwara
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Nucleic Acids Res 34:3389-98. 2006
    ..These results indicate that, besides the involvement in the replication and checkpoint, Dpb11 functions with Ddc1 in the recombination repair process itself...
  23. ncbi request reprint [DNA helicases and progeroid syndromes]
    Shusuke Tada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Seikagaku 78:221-9. 2006
  24. pmc Ctf18 is required for homologous recombination-mediated double-strand break repair
    Hideaki Ogiwara
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Nucleic Acids Res 35:4989-5000. 2007
    ..These results suggest that Ctf18 is involved in damage-induced homologous recombination...
  25. ncbi request reprint Functional relationships between Rad18 and WRNIP1 in vertebrate cells
    Akari Yoshimura
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Biol Pharm Bull 29:2192-6. 2006
    ..Unexpectedly, the severe CPT sensitivity of RAD18-/- cells is slightly suppressed by disruption of the WRNIP1 gene...
  26. ncbi request reprint Chromatin loading of Smc5/6 is induced by DNA replication but not by DNA double-strand breaks
    Takashi Tsuyama
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem Biophys Res Commun 351:935-9. 2006
    ..These findings suggest that the Smc5/6 complex is regulated during the cell cycle, presumably in anticipation of DNA damage that may arise during replication...
  27. ncbi request reprint Activation of a novel pathway involving Mms1 and Rad59 in sgs1 cells
    Ayako Ui
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 356:1031-7. 2007
    ..Lastly, a putative role of Mms1 in the elevation of uSCR and a possible mechanism by which uSCR is elevated as a result of defective Sgs1 and Rad51 are discussed...
  28. doi request reprint Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication
    Takashi Ohuchi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 8:690-6. 2009
    ..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed...
  29. doi request reprint KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation
    Takuya Abe
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Cell Signal 20:1978-85. 2008
    ..These results suggest that the proteins involved in the early stage of NHEJ pathway including Artemis but not the downstream factors decide the cell fate by selecting apoptosis or DNA repair according to the degree of DNA damage...
  30. doi request reprint Rad52 sumoylation and its involvement in the efficient induction of homologous recombination
    Takashi Ohuchi
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    DNA Repair (Amst) 7:879-89. 2008
    ....
  31. ncbi request reprint The INO80 complex is required for damage-induced recombination
    Satoshi Kawashima
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 355:835-41. 2007
    ..Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologous recombination upon DNA damages...
  32. ncbi request reprint Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells
    Yuji Tomizawa
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan
    Genes Genet Syst 82:157-60. 2007
    ..However, UV-induced recombination between homologous chromosomes is intact in both rad50 and smc6-56 mutant cells...
  33. doi request reprint Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2
    Shunya Takada
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 379:233-8. 2009
    ..In contrast to results obtained with mouse and DrosophilaSod2 mutants, we found no indication of an increase in DNA lesions due to depletion of SOD2...
  34. ncbi request reprint A novel role for Rad17 in homologous recombination
    Katsuaki Nishino
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    Genes Genet Syst 83:427-31. 2008
    ..Thus, in addition to its role in the replication checkpoint, Rad17 appears to play a role in homologous recombination...
  35. ncbi request reprint WRN counteracts the NHEJ pathway upon camptothecin exposure
    Makoto Otsuki
    Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Biochem Biophys Res Commun 355:477-82. 2007
    ..The implications for the function of WRN in the non-homologous end-joining pathway of DNA repair involving Ku70 and DNA-PKcs, which may be the cause of lethality in the presence of CPT, will be discussed...
  36. ncbi request reprint Structural basis for inhibition of the replication licensing factor Cdt1 by geminin
    Changwook Lee
    National Creative Research Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo ja dong, San31, Pohang, KyungBook, South Korea
    Nature 430:913-7. 2004
    ....
  37. pmc Geminin becomes activated as an inhibitor of Cdt1/RLF-B following nuclear import
    Ben Hodgson
    Cancer Research UK Chromosome Replication, Research Group, Wellcome Trust Biocentre, University of Dundee, Dow Street, DD1 5EH, Dundee, United Kingdom
    Curr Biol 12:678-83. 2002
    ..Since the initiation of replication at licensed origins depends on nuclear assembly, our results suggest an elegant and novel mechanism for preventing rereplication of DNA in a single cell cycle...
  38. ncbi request reprint Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development
    Ken ichiro Yanagi
    Cellular Physiology Laboratory, Discovery Research Institute, RIKEN, Wako, Saitama, Japan
    J Biol Chem 280:19689-94. 2005
    ..We conclude that the Cdt1-geminin system is conserved throughout metazoans and that geminin has evolved in these taxa to regulate proliferation and differentiation by directly interacting with Cdt1 and homeobox proteins...