Teruo Okano

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. pmc Transscleral sustained vasohibin-1 delivery by a novel device suppressed experimentally-induced choroidal neovascularization
    Hideyuki Onami
    Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine ART, Tohoku University Graduate School of Medicine, Sendai, Japan
    PLoS ONE 8:e58580. 2013
  2. ncbi request reprint Intrascleral transplantation of a collagen sheet with cultured brain-derived neurotrophic factor expressing cells partially rescues the retina from damage due to acute high intraocular pressure
    Toshiaki Abe
    Division of Clinical Cell Therapy, Center for Advanced Medical Research and Development ART, Graduate School of Medicine, Tohoku University, Sendai, Japan
    Adv Exp Med Biol 801:837-43. 2014
  3. ncbi request reprint Correlation of varicella-zoster virus copies and final visual acuities of acute retinal necrosis syndrome
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 236:747-52. 1998
  4. ncbi request reprint Interleukin-1beta and barrier function of retinal pigment epithelial cells (ARPE-19): aberrant expression of junctional complex molecules
    Toshiaki Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Miyagi, Japan
    Invest Ophthalmol Vis Sci 44:4097-104. 2003
  5. ncbi request reprint Transgenic rd mice harboring axokine gene by Rpe65 gene promoter does not rescue photoreceptor degeneration
    Toshiaki Abe
    Department of Opthalmology, Tohoku University, School of Medicine, 1 1 Seiryomachi Aobaku, Sendai Miyagi, 980 8574, Japan
    Adv Exp Med Biol 533:403-6. 2003
  6. doi request reprint Topical doxycycline can induce expression of BDNF in transduced retinal pigment epithelial cells transplanted into the subretinal space
    Toshiaki Abe
    Division of Clinical Cell Therapy, Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Miyagi, Sendai, Japan
    Invest Ophthalmol Vis Sci 49:3631-9. 2008
  7. ncbi request reprint Protection of photoreceptor cells from phototoxicity by transplanted retinal pigment epithelial cells expressing different neurotrophic factors
    Toshiaki Abe
    Division of Clinical Cell Therapy, School of Medicine, Tohoku University, Sendai, Miyagi, Japan
    Cell Transplant 14:799-808. 2005
  8. ncbi request reprint Retinal pigment epithelial cells from thermally responsive polymer-grafted surface reduce apoptosis
    Toshiaki Abe
    Division of Clinical Cell Therapy, Tohoku University, School of Medicine, 1 1 Seiryomachi Aobaku, Sendai Miyagi, 980 8574, Japan
    Adv Exp Med Biol 572:363-6. 2006
  9. ncbi request reprint Ophthalmological findings in patients with spinocerebellar ataxia type 1 are not correlated with neurological anticipation
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 239:722-8. 2001
  10. ncbi request reprint Iris pigment epithelial cell transplantation for degenerative retinal diseases
    Toshiaki Abe
    Division of Clinical Cell Therapy, Center for Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Sendai, Japan
    Prog Retin Eye Res 26:302-21. 2007

Collaborators

Detail Information

Publications96

  1. pmc Transscleral sustained vasohibin-1 delivery by a novel device suppressed experimentally-induced choroidal neovascularization
    Hideyuki Onami
    Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine ART, Tohoku University Graduate School of Medicine, Sendai, Japan
    PLoS ONE 8:e58580. 2013
    ..Choroidal neovascularization can be treated with transscleral sustained protein delivery using our novel device. We offer a safer sustained protein release for treatment of retinal disease using the transscleral approach...
  2. ncbi request reprint Intrascleral transplantation of a collagen sheet with cultured brain-derived neurotrophic factor expressing cells partially rescues the retina from damage due to acute high intraocular pressure
    Toshiaki Abe
    Division of Clinical Cell Therapy, Center for Advanced Medical Research and Development ART, Graduate School of Medicine, Tohoku University, Sendai, Japan
    Adv Exp Med Biol 801:837-43. 2014
    ..Intra-scleral Coll-RPE-BDNF transplantation may partially rescue retinal cells from acute high intraocular pressure. ..
  3. ncbi request reprint Correlation of varicella-zoster virus copies and final visual acuities of acute retinal necrosis syndrome
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 236:747-52. 1998
    ..In spite of aggressive anti-viral therapy, the clinical courses and final visual acuities among these patients have varied. Our purpose was to estimate the amount of virus and compare it to final visual acuity in patients with ARN...
  4. ncbi request reprint Interleukin-1beta and barrier function of retinal pigment epithelial cells (ARPE-19): aberrant expression of junctional complex molecules
    Toshiaki Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Miyagi, Japan
    Invest Ophthalmol Vis Sci 44:4097-104. 2003
    ..To examine the effects of interleukin (IL)-1beta on the resistance and permeability of the tight junctions of cultured retinal pigment epithelial cells...
  5. ncbi request reprint Transgenic rd mice harboring axokine gene by Rpe65 gene promoter does not rescue photoreceptor degeneration
    Toshiaki Abe
    Department of Opthalmology, Tohoku University, School of Medicine, 1 1 Seiryomachi Aobaku, Sendai Miyagi, 980 8574, Japan
    Adv Exp Med Biol 533:403-6. 2003
  6. doi request reprint Topical doxycycline can induce expression of BDNF in transduced retinal pigment epithelial cells transplanted into the subretinal space
    Toshiaki Abe
    Division of Clinical Cell Therapy, Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Miyagi, Sendai, Japan
    Invest Ophthalmol Vis Sci 49:3631-9. 2008
    ..To determine whether topical doxycycline (DOX) induces the expression of brain-derived neurotrophic factor (BDNF) by BDNF-transduced retinal pigment epithelial (RPE) cells transplanted into the subretinal space of rats...
  7. ncbi request reprint Protection of photoreceptor cells from phototoxicity by transplanted retinal pigment epithelial cells expressing different neurotrophic factors
    Toshiaki Abe
    Division of Clinical Cell Therapy, School of Medicine, Tohoku University, Sendai, Miyagi, Japan
    Cell Transplant 14:799-808. 2005
    ..We conclude that the transplantation of BDNF-transduced RPE cells can reduce the photoreceptor damage induced by phototoxicity in the transplanted area and weakly in the nontransplanted area...
  8. ncbi request reprint Retinal pigment epithelial cells from thermally responsive polymer-grafted surface reduce apoptosis
    Toshiaki Abe
    Division of Clinical Cell Therapy, Tohoku University, School of Medicine, 1 1 Seiryomachi Aobaku, Sendai Miyagi, 980 8574, Japan
    Adv Exp Med Biol 572:363-6. 2006
  9. ncbi request reprint Ophthalmological findings in patients with spinocerebellar ataxia type 1 are not correlated with neurological anticipation
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 239:722-8. 2001
    ..The purpose of this study was to determine whether the ophthalmological findings are correlated with the neurological disorders, and whether ophthalmological anticipation is present in patients with SCA1...
  10. ncbi request reprint Iris pigment epithelial cell transplantation for degenerative retinal diseases
    Toshiaki Abe
    Division of Clinical Cell Therapy, Center for Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Sendai, Japan
    Prog Retin Eye Res 26:302-21. 2007
    ..Furthermore, transplantations of IPE cells have been performed to treat central nervous system disorders. In this review, we summarize recent progress on IPE transplantation...
  11. ncbi request reprint Dedifferentiation of the retinal pigment epithelium compared to the proliferative membranes of proliferative vitreoretinopathy
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Miyagi, Japan
    Curr Eye Res 17:1103-9. 1998
    ..To examine the gene expression for melanogenesis of retinal pigment epithelial cells during dedifferentiation and to compare the condition to that of eyes obtaining anatomical success after surgery for proliferative vitreoretinopathy...
  12. ncbi request reprint Characterization of iris pigment epithelial cell for auto cell transplantation
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Miyagi, Japan
    Cell Transplant 8:501-10. 1999
    ..In the present study, we established auto IPE cell culture using auto serum. The cultured IPE cell showed pigment epithelial cell properties until around five passages in both human and monkey...
  13. ncbi request reprint Cytokine gene expression after subretinal transplantation
    T Abe
    Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
    Tohoku J Exp Med 189:179-89. 1999
    ..These cytokines may play an important role for the local reaction after transplantation...
  14. ncbi request reprint Functional analysis after auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Japan
    Tohoku J Exp Med 189:295-305. 1999
    ..From this functional analysis, transplanted autologous IPE may have, in part, an alternative function in regard to the retinal pigment epithelium in the subretinal space...
  15. ncbi request reprint Autologous iris pigment epithelial cell transplantation in monkey subretinal region
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Japan
    Curr Eye Res 20:268-75. 2000
    ..To establish autologous iris pigment epithelial (IPE) cell transplantation in monkey eyes...
  16. ncbi request reprint Auto iris pigment epithelial cell transplantation in patients with age-related macular degeneration: short-term results
    T Abe
    Department of Ophthalmology, Tohoku University, School of Medicine, Sendai, Japan
    Tohoku J Exp Med 191:7-20. 2000
    ..In conclusion, the treatment resulted in no significant improvement in macular function, as compared with the control; however, no rejection or deterioration in visual acuity occurred up to the 13 month follow up...
  17. ncbi request reprint Visual function after removal of subretinal neovascular membranes in patients with age-related macular degeneration
    T Abe
    Department of Ophthalmology, School of Medicine, Sendai, Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 239:927-36. 2001
    ....
  18. ncbi request reprint Interferon gamma expression and clinical features in patients with acute retinal necrosis syndrome
    Toshiaki Abe
    Department of Ophthalmology, School of Medicine, Tohoku University, Sendai, 980 8574 Miyagi, Japan
    Graefes Arch Clin Exp Ophthalmol 241:982-7. 2003
    ..The purpose of this study was to examine the relationship between IFN-gamma expression and the clinical course of patients with acute retinal necrosis syndrome (ARN) associated with the varicella-zoster virus (VZV)...
  19. ncbi request reprint Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface
    Yasutaka Hayashida
    Department of Ophthalmology, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Invest Ophthalmol Vis Sci 46:1632-9. 2005
    ....
  20. doi request reprint A protocol for immunofluorescence staining of floating neurospheres
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, and Global Center of Excellence COE Program, Tokyo Women s Medical University, School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Neurosci Lett 479:126-7. 2010
    ..PBS (400microL) is added to the well for washing the spheres. This procedure is repeated three times. This protocol is easier than a conventional procedure using cryostat sections and can give clear sphere structures...
  21. doi request reprint Histological evaluation of mechanical epithelial separation in epithelial laser in situ keratomileusis
    Takeshi Soma
    Department of Ophthalmology, Osaka University Medical School, Osaka, Japan
    J Cataract Refract Surg 35:1251-9. 2009
    ..To evaluate the effect of mechanical epithelial separation with an epikeratome on the histologic ultrastructure of epithelial flaps and stromal beds from human corneas...
  22. doi request reprint PLGA artificial nerve conduits with dental pulp cells promote facial nerve regeneration
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, Tokyo Women s Medical University, School of Medicine, Tokyo, Japan
    J Tissue Eng Regen Med 5:823-30. 2011
    ..PLGA tubes filled with DPCs promoted nerve regeneration and were readily resorbed in vivo...
  23. ncbi request reprint Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze
    Yasutaka Hayashida
    Department of Ophthalmology, Osaka University Medical School, Osaka, Japan
    Invest Ophthalmol Vis Sci 47:552-7. 2006
    ..To apply tissue-engineered cell sheet transplantation after excimer laser keratectomy as a novel approach for the reduction of postoperative corneal haze...
  24. ncbi request reprint N-Cadherin is expressed by putative stem/progenitor cells and melanocytes in the human limbal epithelial stem cell niche
    Ryuhei Hayashi
    Department of Ophthalmology, Tohoku University School of Medicine, 1 1 Seiryomachi, Aoba ku, Sendai 980 8574, Japan
    Stem Cells 25:289-96. 2007
    ..These results indicate that N-cadherin may be a critical cell-to-cell adhesion molecule between corneal epithelial stem/progenitor cells and their corresponding niche cells in the limbal epithelium...
  25. doi request reprint Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells
    Hiroaki Sugiyama
    Department of Ophthalmology, Osaka University Medical School, Osaka 565 0871, Japan
    J Tissue Eng Regen Med 2:445-9. 2008
    ..Using the mesenchymal stem cells as novel feeder layers, transplantable epithelial cell sheets could be effectively generated ex vivo on temperature-responsive cell-culture surfaces...
  26. ncbi request reprint Analysis of angiogenesis induced by cultured corneal and oral mucosal epithelial cell sheets in vitro
    Shintaro Kanayama
    Department of Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
    Exp Eye Res 85:772-81. 2007
    ..In conclusion, angiogenic potential of COE is greater than that of CCE and FGF2 is a candidate involved in the induction of corneal neovascularization after COE sheet transplantation...
  27. ncbi request reprint Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium
    Kohji Nishida
    Department of Ophthalmology, Osaka University Medical School, Suita, Osaka, Japan
    N Engl J Med 351:1187-96. 2004
    ..We studied the use of autologous oral mucosal epithelial cells as a source of cells for the reconstruction of the corneal surface...
  28. ncbi request reprint Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces
    Taizo Sumide
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
    FASEB J 20:392-4. 2006
    ..Our results support the value of harvested cell sheets for clinical applications in ocular reconstructive surgery in patients with ocular endothelial decompensation...
  29. ncbi request reprint Development of transplantable genetically modified corneal epithelial cell sheets for gene therapy
    Katsuhiko Watanabe
    Department of Ophthalmology, Osaka University Medical School, Room E7, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biomaterials 28:745-9. 2007
    ..Immunofluorescence data showed that p63 was also expressed in the basal layer of the same cell sheet. These results suggest that this technique will likely be applicable to ex vivo gene therapies for various corneal disorders...
  30. ncbi request reprint Enrichment of corneal epithelial stem/progenitor cells using cell surface markers, integrin alpha6 and CD71
    Ryuhei Hayashi
    Department of Ophthalmology, Tohoku University School of Medicine, 1 1 Seiryomachi, Aoba ku, Sendai, Miyagi, Japan
    Biochem Biophys Res Commun 367:256-63. 2008
    ..The cells were localized immunohistochemically in the basal region of the limbal epithelium. These results indicate that the alpha(6)(bri)/CD71(dim) subpopulation enriched corneal epithelial stem cells...
  31. doi request reprint Neurosphere generation from dental pulp of adult rat incisor
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, Tokyo Women s Medical University, Graduate School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Eur J Neurosci 27:538-48. 2008
    ..These data suggest that the CD81 and nestin double-positive cells localized in the odontoblast layer of the apical portion of the dental pulp may have the ability to grow and form neurospheres...
  32. doi request reprint Tubulation with dental pulp cells promotes facial nerve regeneration in rats
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Tokyo Women s Medical University, Tokyo, Japan
    Tissue Eng Part A 14:1141-7. 2008
    ..These results suggest that the transplanted dental pulp cells formed blood vessels and myelinating tissue and contributed to the promotion of normal nerve regeneration...
  33. ncbi request reprint Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG2
    Katsuhiko Watanabe
    Department of Ophthalmology, Osaka University Medical School, Room E7, Yamadaoka 2 2, Suita, Osaka 565 0871, Japan
    FEBS Lett 565:6-10. 2004
    ..Immunofluorescence revealed that a portion of limbal epithelial basal cells expressed ABCG2. Data indicate that ABCG2 positive limbal epithelial cells are putative corneal epithelial stem cells...
  34. doi request reprint Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation
    Yuji Tanaka
    Department of Ophthalmology, Tohoku University Graduate School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai, Miyagi 980 8574, Japan
    Biomaterials 32:6764-72. 2011
    ..e., inflammation, vascularization, and the barrier defect of epithelia) or no optical functional change on optically cleared dermis was observed during its 4-week autogenic transplantation into rabbit corneal stromal pocket...
  35. ncbi request reprint Validation system of tissue-engineered epithelial cell sheets for corneal regenerative medicine
    Ryuhei Hayashi
    Department of Ophthalmology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Tissue Eng Part C Methods 16:553-60. 2010
    ..Further, these cells also lost epithelial barrier function. These results showed the potential effectiveness of the proposed validation system that can evaluate fabricated cell sheets before transplantation...
  36. ncbi request reprint Structural characterization of bioengineered human corneal endothelial cell sheets fabricated on temperature-responsive culture dishes
    Takeshi Ide
    Department of Ophthalmology, Osaka University Medical School, Osaka, 2 2 Yamadaoka, Rm E7, Suita, Osaka 565 0871, Japan
    Biomaterials 27:607-14. 2006
    ..These results suggest that these transplantable corneal endothelial cell sheets can be applied to treat patients with damaged corneas...
  37. doi request reprint Irreversible optical clearing of sclera by dehydration and cross-linking
    Yuji Tanaka
    Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai, Miyagi 980 8574, Japan
    Biomaterials 32:1080-90. 2011
    ..Wet transparent cross-linked sclera can also show a characteristic strong tensile strength. Furthermore, rabbit corneal epithelium has regenerated on the transparent sclera with cross-linking in vitro...
  38. doi request reprint In vivo 3D analysis with micro-computed tomography of rat calvaria bone regeneration using periosteal cell sheets fabricated on temperature-responsive culture dishes
    Hiroto Uchiyama
    Department of Oral and Maxillofacial Surgery, School of Medicine, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    J Tissue Eng Regen Med 5:483-90. 2011
    ..With further possible improvements, scaffold-free periosteal cell sheet fabricated on temperature-responsive culture dishes will be a valuable method for inducing and accelerating bone regeneration...
  39. ncbi request reprint Transplantable retinal pigment epithelial cell sheets for tissue engineering
    Akira Kubota
    Department of Ophthalmology, Osaka University Medical School, Room E7, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Biomaterials 27:3639-44. 2006
    ..Therefore, these methods provide significant insight into the fabrication of transplantable RPE cell sheets that can be applied to RPE regenerative therapies to restore lost vision...
  40. doi request reprint Punch and spindle-shaped biopsies for collecting oral mucosal tissue for the fabrication of transplantable autologous epithelial cell sheets
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery and Global Center of Excellence COE Program, Tokyo Women s Medical University, School of Medicine, Tokyo, Japan
    J Biomed Mater Res A 100:2849-54. 2012
    ....
  41. doi request reprint Transplantation of tissue-engineered retinal pigment epithelial cell sheets in a rabbit model
    Naoko Yaji
    Department of Ophthalmology, Tokyo Women s Medical University, Tokyo, Japan
    Biomaterials 30:797-803. 2009
    ..We conclude that tissue-engineered RPE cell sheets harvested from temperature-responsive culture dishes can be effectively transplanted beneath the neural retina...
  42. doi request reprint Transparent, tough collagen laminates prepared by oriented flow casting, multi-cyclic vitrification and chemical cross-linking
    Yuji Tanaka
    Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Aoba ku, Sendai, Miyagi, Japan
    Biomaterials 32:3358-66. 2011
    ..The tensile strength of laminates is extremely higher than any other engineered materials as well as native cornea, which exhibit an orthogonal laminated collagen structure and a good optical transmission...
  43. ncbi request reprint Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface
    Kohji Nishida
    Department of Ophthalmology, Osaka University Medical School, Suita, Japan
    Transplantation 77:379-85. 2004
    ..We have developed a novel cell-sheet manipulation technology using temperature-responsive culture surfaces to generate functional, cultivated corneal epithelial cell sheet grafts...
  44. doi request reprint Spontaneous fibrosarcoma in an experimental aged Lewis rat
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, and Global Centre of Excellence Program, Tokyo Women s Medical University, School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Lab Anim 46:352-5. 2012
    ..From the results of these analyses, the final pathological diagnosis of this tumour was a fibrosarcoma...
  45. doi request reprint Surgical anatomy of the swine face
    Ryo Sasaki
    Department of Oral and Maxillofacial Surgery, and Global Center of Excellence COE Program, School of Medicine, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Lab Anim 44:359-63. 2010
    ....
  46. ncbi request reprint Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells
    T Ohashi
    Graduate School of Mechanical Engineering, Tohoku University, Sendai, Japan
    Biomed Mater Eng 12:319-27. 2002
    ..This difference can be attributable to the differences in approximation functions to determine the elastic modulus. The elastic modulus would contribute a better understanding of local mechanical properties of the cells...
  47. ncbi request reprint Comparison of spinal cord gray matter and white matter softness: measurement by pipette aspiration method
    H Ozawa
    Department of Orthopaedic Surgery, Tohoku University School of Medicine, Aobaku, Sendai, Japan
    J Neurosurg 95:221-4. 2001
    ..The modulis of elasticity of the gray and white matter were measured in situ by using a pipette aspiration method...
  48. ncbi request reprint Application of scanning acoustic microscopy for assessing stress distribution in atherosclerotic plaque
    Y Saijo
    Department of Medical Engineering and Cardiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
    Ann Biomed Eng 29:1048-53. 2001
    ..SAM provides information on the physical properties, which cannot be obtained by the optical microscope. The results would help in understanding the pathological features of atherosclerosis...
  49. doi request reprint Vascularized versus nonvascularized island median nerve grafts in the facial nerve regeneration and functional recovery of rats for facial nerve reconstruction study
    Hajime Matsumine
    Department of Plastic and Reconstructive Surgery, Tokyo Women s Medical University, School of Medicine, Tokyo, Japan
    J Reconstr Microsurg 30:127-36. 2014
    ..Compound muscle action potential measurement showed that the parameters of vascularized group were similar to those in the intact control group. A vascularized median nerve graft resulted in better facial nerve regeneration outcomes. ..
  50. doi request reprint Induced adipocyte cell-sheet ameliorates cardiac dysfunction in a mouse myocardial infarction model: a novel drug delivery system for heart failure
    Yukiko Imanishi
    Department of Cardiovascular Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
    Circulation 124:S10-7. 2011
    ....
  51. doi request reprint Preparation of thermoresponsive cationic copolymer brush surfaces and application of the surface to separation of biomolecules
    Kenichi Nagase
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawadacho, Shinjuku, Tokyo 162 8666, Japan
    Biomacromolecules 9:1340-7. 2008
    ..These results indicate that cationic copolymer brush surfaces prepared by ATRP can rapidly alter their electrostatic properties by changing aqueous temperature...
  52. doi request reprint Transportation of transplantable cell sheets fabricated with temperature-responsive culture surfaces for regenerative medicine
    Takayuki Nozaki
    Advanced Research Laboratory, Hitachi Ltd, 2520 Akanuma, Hiki gun Hatoyama cho, Saitama 350 0395, Japan
    J Tissue Eng Regen Med 2:190-5. 2008
    ..During the transportation, cell viability and histology were not impaired. This unique transportation device would be useful for cell sheet-based regenerative medicine utilizing temperature-responsive culture surfaces...
  53. ncbi request reprint Cellular control of tissue architectures using a three-dimensional tissue fabrication technique
    Yukiko Tsuda
    Graduate School of Pure and Applied Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki, Japan
    Biomaterials 28:4939-46. 2007
    ..This novel technique holds promise for the study of cell-cell communications and angiogenesis in reconstructed, three-dimensional environments as well as for the fabrication of tissues with complex, multicellular architecture...
  54. ncbi request reprint Effects of graft densities and chain lengths on separation of bioactive compounds by nanolayered thermoresponsive polymer brush surfaces
    Kenichi Nagase
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawadacho, Shinjuku, Tokyo 162 8666, Japan
    Langmuir 24:511-7. 2008
    ....
  55. pmc A thermoresponsive, microtextured substrate for cell sheet engineering with defined structural organization
    Brett C Isenberg
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, MA 02215, USA
    Biomaterials 29:2565-72. 2008
    ..Ultimately, such sheets will be layered to form larger tissues with defined ECM/cell organization that spans multiple length scales...
  56. ncbi request reprint Reconstruction of functional tissues with cell sheet engineering
    Joseph Yang
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Biomaterials 28:5033-43. 2007
    ..Cell sheet engineering therefore provides a novel alternative for regenerative medicine approaches that require the re-creation of functional tissue structures...
  57. ncbi request reprint The use of biotin-avidin binding to facilitate biomodification of thermoresponsive culture surfaces
    Masanori Nishi
    Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1 3 Kagurazaka, Shinjuku, Tokyo, Japan
    Biomaterials 28:5471-6. 2007
    ..Consequently, by optimizing the spacer length, biomolecules can be functionally immobilized onto thermoresponsive surfaces via the affinity binding between avidin and biotin...
  58. doi request reprint Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase
    Hideko Kanazawa
    Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, 1 5 30 Shibakoen, Minato ku, Tokyo 105 8512, Japan
    J Chromatogr A 1191:157-61. 2008
    ..The electrostatic and hydrophobic interactions could be modulated simultaneously with the temperature in an aqueous mobile phase, thus the separation system would have potential applications in the separation of biomolecules...
  59. ncbi request reprint Microfluidic devices for size-dependent separation of liver cells
    Masumi Yamada
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo, 162 8666, Japan
    Biomed Microdevices 9:637-45. 2007
    ..The results showed that the presented microfluidic devices are low-cost and suitable for clinical use, and capable of highly functional separation with relatively high-speed processing...
  60. ncbi request reprint Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment
    Hideyuki Hatakeyama
    Institute of Advanced Biomedical Engineering and Science, Center of Excellence Program for the 21st Century, Tokyo Women s Medical University, Shinjuku, Tokyo, Japan
    Biomaterials 28:3632-43. 2007
    ..Thus, patterned biofunctional designs would be utilized for the creation and harvest of biomimetic-designed vascular networks having sufficient biofunctional activities in facilitated cell sheet engineering and regenerative medicine...
  61. ncbi request reprint Temperature-responsive polymeric carriers incorporating hydrophobic monomers for effective transfection in small doses
    Naoya Takeda
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku, Tokyo 162 8666, Japan
    J Control Release 95:343-55. 2004
    ....
  62. ncbi request reprint Cross-linked thermoresponsive anionic polymer-grafted surfaces to separate bioactive basic peptides
    Jun Kobayashi
    Department of Applied Chemistry, Waseda University, 3 4 1 Ohkubo, Shinjuku, Tokyo 169 8555, Japan
    Anal Chem 75:3244-9. 2003
    ..In conclusion, these anionic thermoresponsive polymer-modified surfaces are good candidates for improved separation of bioactive peptides under exclusively aqueous conditions...
  63. ncbi request reprint Bioengineered cardiac cell sheet grafts have intrinsic angiogenic potential
    Sachiko Sekiya
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Biochem Biophys Res Commun 341:573-82. 2006
    ..In conclusion, myocardial tissue grafts engineered with cell sheet technology have their own inherent potential for the in vivo neovascularization that can be regulated in vitro...
  64. ncbi request reprint Study of temperature-responsibility on the surfaces of a thermo-responsive polymer modified stationary phase
    Eri Ayano
    Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, Minato ku, Tokyo, Japan
    J Chromatogr A 1119:51-7. 2006
    ..This system should be highly useful to control the function and property of the stationary phase for HPLC only by changing the temperature with an aqueous solvent...
  65. ncbi request reprint Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction
    Yoshinori Miyahara
    Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
    Nat Med 12:459-65. 2006
    ..Thus, transplantation of monolayered mesenchymal stem cells may be a new therapeutic strategy for cardiac tissue regeneration...
  66. ncbi request reprint Interfacial property modulation of thermoresponsive polymer brush surfaces and their interaction with biomolecules
    Kenichi Nagase
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, Kawadacho, Shinjuku, Tokyo, Japan
    Langmuir 23:9409-15. 2007
    ....
  67. ncbi request reprint Regulation of protein binding toward a ligand on chromatographic matrixes by masking and forced-releasing effects using thermoresponsive polymer
    Kimihiro Yoshizako
    Japan Chemical Innovation Institute, Tsukuba, Ibaraki
    Anal Chem 74:4160-6. 2002
    ..Selective recovery of human albumin from human sera was demonstrated using this newly developed thermoresponsive affinity column...
  68. ncbi request reprint Expression of Integrin beta3 is correlated to the properties of quiescent hemopoietic stem cells possessing the side population phenotype
    Terumasa Umemoto
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    J Immunol 177:7733-9. 2006
    ....
  69. ncbi request reprint Cell delivery in regenerative medicine: the cell sheet engineering approach
    Joseph Yang
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, Tokyo, Japan
    J Control Release 116:193-203. 2006
    ....
  70. ncbi request reprint Molecular design of biodegradable polymeric micelles for temperature-responsive drug release
    Masamichi Nakayama
    Tokyo Women s Medical University, Institute of Advanced Biomedical Engineering and Science, Kawada cho 8 1, Shinjuku ku, Tokyo 162 8666, Japan
    J Control Release 115:46-56. 2006
    ..One polymeric micelle composition successfully exhibited rapid and thermo-responsive drug release while possessing a biodegradable character...
  71. ncbi request reprint Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture
    Masayuki Yamato
    Institute of Biomedical Engineering, Tokyo Women s Medical University, Japan
    Biomaterials 23:561-7. 2002
    ..Long-term cell pattern fidelity and differentiated cell functions characteristic of each co-planar cell type are observed. This method is simple and has few limitations, compared with other existing co-culture methods...
  72. ncbi request reprint Temperature- and pH-responsive aminopropyl-silica ion-exchange columns grafted with copolymers of N-isopropylacrylamide
    Chikako Sakamoto
    Department of Physical Chemistry, Kyoritsu College of Pharmacy, 1 5 30 Shibakoen, Minato ku, Tokyo 105 8512, Japan
    J Chromatogr A 1030:247-53. 2004
    ..Environmentally-responsive chromatography is expected to be applicable to the separation of pharmaceuticals and biomolecules, such as peptides, proteins and nucleic acids...
  73. ncbi request reprint Two-dimensional cell sheet manipulation of heterotypically co-cultured lung cells utilizing temperature-responsive culture dishes results in long-term maintenance of differentiated epithelial cell functions
    Maya A Nandkumar
    Institute of Biomedical Engineering, Tokyo Women s Medical University, Japan
    Biomaterials 23:1121-30. 2002
    ..The active dome formation also observed subsequent to transfer reaffirms the uniqueness of the culture conditions and 2D CSM in future for developing tissue like architecture in vitro...
  74. ncbi request reprint Temperature-responsive cell culture surfaces enable "on-off" affinity control between cell integrins and RGDS ligands
    Mitsuhiro Ebara
    Department of Applied Chemistry, Waseda University, 3 4 1 Ohkubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biomacromolecules 5:505-10. 2004
    ..Furthermore, the method allows serum-free cell culture and trypsin-free cell harvest, essentially removing mammalian-sourced components from the culture process...
  75. ncbi request reprint Cell micropatterning using photopolymerization with a liquid crystal device commercial projector
    Kazuyoshi Itoga
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Biomaterials 25:2047-53. 2004
    ..Selective cell adhesion control was also achieved on the micropatterned surfaces...
  76. ncbi request reprint Temperature-responsive chromatography using poly(N-isopropylacrylamide) hydrogel-modified silica
    Hideko Kanazawa
    Kyoritsu College of Pharmacy, Minato, Tokyo, Japan
    Anal Sci 18:45-8. 2002
    ..A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields...
  77. ncbi request reprint [Nanosurface modification by grafting temperature-responsive polymers]
    Masayuki Yamato
    Tanpakushitsu Kakusan Koso 48:1602-8. 2003
  78. ncbi request reprint Cell sheet engineering for myocardial tissue reconstruction
    Tatsuya Shimizu
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Biomaterials 24:2309-16. 2003
    ..Cell sheet engineering should have enormous potential for fabricating clinically applicable myocardial tissues and should promote tissue engineering research fields...
  79. ncbi request reprint Process design for efficient and controlled drug incorporation into polymeric micelle carrier systems
    Fukashi Kohori
    Department of Applied Chemistry, Waseda University, 3 4 1, Ohkubo, Shinjuku ku, 169 8555, Tokyo, Japan
    J Control Release 78:155-63. 2002
    ..These results indicate that the efficient incorporation of ADR can be achieved in consideration of the dynamic micelle formation and drug incorporation processes...
  80. ncbi request reprint Copolymerization of 2-carboxyisopropylacrylamide with N-isopropylacrylamide accelerates cell detachment from grafted surfaces by reducing temperature
    Mitsuhiro Ebara
    Department of Applied Chemistry, Waseda University, 3 4 1 Ohkubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biomacromolecules 4:344-9. 2003
    ....
  81. ncbi request reprint Micropatterned surfaces prepared using a liquid crystal projector-modified photopolymerization device and microfluidics
    Kazuyoshi Itoga
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, CREST JST, Kawada cho 8 1, Shinjuku ku, Tokyo 162 8666, Japan
    J Biomed Mater Res A 69:391-7. 2004
    ..This technique should prove useful for inexpensive, rapid prototyping of surface micropatterns from polymer materials...
  82. ncbi request reprint Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and noninvasive cell harvest
    Mitsuhiro Ebara
    Department of Applied Chemistry, Waseda University, Tokyo, Japan
    Tissue Eng 10:1125-35. 2004
    ..These surfaces would be useful for serumfree culture for tissue-engineering applications...
  83. ncbi request reprint Separation of nucleotides with an aqueous mobile phase using pH- and temperature-responsive polymer modified packing materials
    Eri Ayano
    Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, Minato, Tokyo, Japan
    Anal Sci 22:539-43. 2006
    ..A pH- and temperature-responsive chromatography would be greatly useful for biopolymer and nucleotide separation and purification...
  84. ncbi request reprint Heterotypic cell interactions on a dually patterned surface
    Yukiko Tsuda
    Graduate School of Pure and Applied Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba, Ibaraki 305 8571, Japan
    Biochem Biophys Res Commun 348:937-44. 2006
    ..These findings are important for the reconstruction of complex tissues such as liver and pancreas...
  85. ncbi request reprint Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture dishes
    Haruko Obokata
    Graduate School of Science and Engineering, Waseda University, 3 4 1 Ohkubo, Shinjuku ku, Tokyo 169 8555, Japan
    J Biomed Mater Res A 86:1088-96. 2008
    ....
  86. ncbi request reprint The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets
    Daisuke Murakami
    Graduate School of Science and Engineering, Waseda University, Tokyo 169 0051, Japan
    Biomaterials 27:5518-23. 2006
    ..These results suggest that the maintenance of stem/progenitor cells is influenced by the apical/basal supply of nutrients as well as culture supplements...
  87. doi request reprint Preparation of thermoresponsive polymer brush surfaces and their interaction with cells
    Aya Mizutani
    Department of Pharmacy, Kyoritsu University of Pharmacy, 1 5 14 Shibakoen, Minato, Tokyo 105 0011, Japan
    Biomaterials 29:2073-81. 2008
    ..Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications...
  88. ncbi request reprint Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups
    Eri Ayano
    Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, Minato ku, Tokyo, Japan
    J Chromatogr A 1119:58-65. 2006
    ..PH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules...
  89. doi request reprint Millisecond treatment of cells using microfluidic devices via two-step carrier-medium exchange
    Masumi Yamada
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo, 162 8666, Japan
    Lab Chip 8:772-8. 2008
    ..The obtained results demonstrated the ability of the presented system to conduct the rapid stimulation of cells, which would be useful for the analysis of biochemical reactions at the cell surface...
  90. doi request reprint Successful in vivo propagation of factor IX-producing hepatocytes in mice: potential for cell-based therapy in haemophilia B
    Kohei Tatsumi
    Department of Pediatrics, Nara Medical University, Nara, Japan
    Thromb Haemost 99:883-91. 2008
    ..In all, the results from the present study indicated that developments based on this technology could provide sufficient FIX-producing hepatocytes for cell-based therapy for haemophilia B...
  91. ncbi request reprint Cell sheet engineering: recreating tissues without biodegradable scaffolds
    Joseph Yang
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, Tokyo, Japan
    Biomaterials 26:6415-22. 2005
    ..Cell sheet engineering thus presents several significant advantages and can overcome many of the problems that have previously restricted tissue engineering with biodegradable scaffolds...
  92. ncbi request reprint Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line
    Yuji Ishibashi
    Bionic Materials Technology Group, Biomaterials Center, National Institute for Materials Science, Ibaraki, Japan
    Glycoconj J 22:53-62. 2005
    ....
  93. ncbi request reprint Fabrication of a cell array on ultrathin hydrophilic polymer gels utilising electron beam irradiation and UV excimer laser ablation
    Shintaroh Iwanaga
    Department of Applied Chemistry, Waseda University, Tokyo, Japan
    Biomaterials 26:5395-404. 2005
    ..The surface fabrication techniques presented here can be utilised for the preparation of cell-based biosensors as well as tissue engineering constructs...
  94. doi request reprint Influence of graft interface polarity on hydration/dehydration of grafted thermoresponsive polymer brushes and steroid separation using all-aqueous chromatography
    Kenichi Nagase
    Institute of Advanced Biomedical Engineering and Science, Tokyo Women s Medical University, 8 1 Kawadacho, Shinjuku, Tokyo 162 8666, Japan
    Langmuir 24:10981-7. 2008
    ....
  95. ncbi request reprint The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets
    Yukiko Tsuda
    Graduate School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
    Biomaterials 26:1885-93. 2005
    ....
  96. ncbi request reprint Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly (N-isopropylacrylamide) temperature-responsive surfaces
    Terry O Collier
    Department of Macromolecular Science, Case Western Reserve University, 2085 Adelbert Rd, Cleveland, OH 44106, USA
    J Biomed Mater Res 59:136-43. 2002
    ..These novel surfaces allow investigation of the adhesive behavior of adherent inflammatory cells in a temporal manner, and the effects of surface conformation and wettability changes on cell adhesion and detachment...