Norihiko Ohbayashi

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. Kuchitsu Y, Homma Y, Fujita N, Fukuda M. Rab7 knockout unveils regulated autolysosome maturation induced by glutamine starvation. J Cell Sci. 2018;131: pubmed publisher
    ..Such multistep regulatory mechanisms would enable efficient autophagic flux during starvation. ..
  2. Yatsu A, Shimada H, Ohbayashi N, Fukuda M. Rab40C is a novel Varp-binding protein that promotes proteasomal degradation of Varp in melanocytes. Biol Open. 2015;4:267-75 pubmed publisher
    ..These findings indicated that Rab40C is a previously unexpected regulator of Tyrp1 trafficking in melanocytes through controlling the proteasomal degradation of Varp. ..
  3. Ohishi Y, Kinoshita R, Marubashi S, Ishida M, Fukuda M. The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J Biol Chem. 2019;294:6912-6922 pubmed publisher
    ..These results indicate that activation of Rab32/38 by HPS4 (or BLOC-3) is essential for melanogenesis of cultured melanocytes and that Rab9 likely regulates melanogenesis independently of HPS4. ..
  4. Etoh K, Fukuda M. Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J Cell Sci. 2019;132: pubmed publisher
    ..Our findings provide insight into the mechanism by which the Rab10-KIF13A (or KIF13B) complex regulates tubular endosome formation. This article has an associated First Person interview with the first author of the paper. ..
  5. Kuchitsu Y, Fukuda M. Revisiting Rab7 Functions in Mammalian Autophagy: Rab7 Knockout Studies. Cells. 2018;7: pubmed publisher
    ..In this review article, we describe novel roles of Rab7 in mammalian autophagy and discuss its functional diversification during evolution. ..
  6. Matsui T, Fukuda M. Methods of analysis of the membrane trafficking pathway from recycling endosomes to lysosomes. Methods Enzymol. 2014;534:195-206 pubmed publisher
    ..This chapter summarizes the methods that the authors used to analyze the membrane trafficking pathway from recycling endosomes to lysosomes that is specifically regulated by Rab12. ..
  7. Yasuda T, Homma Y, Fukuda M. Slp2-a inactivates ezrin by recruiting protein phosphatase 1 to the plasma membrane. Biochem Biophys Res Commun. 2015;460:896-902 pubmed publisher
    ..These results indicate that Slp2-a inactivates ezrin by recruiting PP1 to the plasma membrane. ..
  8. Homma Y, Fukuda M. Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol Biol Cell. 2016;27:2107-18 pubmed publisher
    ..These localizations and functions of Rabin8 are Rab11 dependent. Thus Rabin8 regulates neurite outgrowth both by coordinating with Rab8, Rab10, and Rab11 and by a GEF activity-independent mechanism. ..
  9. Aizawa M, Fukuda M. Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J Biol Chem. 2015;290:22250-61 pubmed publisher
    ..Our findings suggest that the compacted Golgi morphology of mammalian cells is finely tuned by multiple sets of Rab (or Rab-effector complexes) that for the most part function independently. ..

More Information

Publications20

  1. Oguchi M, Etoh K, Fukuda M. Rab20, a novel Rab small GTPase that negatively regulates neurite outgrowth of PC12 cells. Neurosci Lett. 2018;662:324-330 pubmed publisher
  2. Mrozowska P, Fukuda M. Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J Cell Biol. 2016;213:355-69 pubmed publisher
    ..Our results give substantial insight into regulation of the transcytosis of this apical marker and highlight differences between trafficking mechanisms in 2D and 3D cell cultures. ..
  3. Marubashi S, Ohbayashi N, Fukuda M. A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J Invest Dermatol. 2016;136:1672-1680 pubmed publisher
    ..These findings indicated that RACK1 competes with Rab40C for binding to the ANKR2 domain of Varp and regulates dendrite outgrowth through stabilization of Varp in mouse melanocytes. ..
  4. Fukuda M. TBC proteins: GAPs for mammalian small GTPase Rab?. Biosci Rep. 2011;31:159-68 pubmed publisher
    ..g. leanness in mice and insulin resistance in humans). The present study reviews the structure and function of mammalian TBC proteins, especially in relation to Rab small GTPases. ..
  5. Etoh K, Fukuda M. Structure-function analyses of the small GTPase Rab35 and its effector protein centaurin-β2/ACAP2 during neurite outgrowth of PC12 cells. J Biol Chem. 2015;290:9064-74 pubmed publisher
  6. Kobayashi H, Etoh K, Ohbayashi N, Fukuda M. Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth. Biol Open. 2014;3:803-14 pubmed publisher
    ..Our findings suggest the existence of a novel mechanism that recruits multiple Rab proteins at the Arf6-positive compartment by MICAL-L1. ..
  7. Matsui T, Noguchi K, Fukuda M. Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts. J Biol Chem. 2014;289:13986-95 pubmed publisher
    ..These findings indicated that Dennd3 not only functions as a Rab12-GEF but also modulates Akt signaling in mouse embryonic fibroblast cells. ..
  8. Ohbayashi N, Maruta Y, Ishida M, Fukuda M. Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J Cell Sci. 2012;125:1508-18 pubmed publisher
    ..These findings reveal a new mechanism by which the dynein-dynactin motor complex recognizes Mreg on mature melanosomes through interaction with RILP and is involved in the centripetal movement of melanosomes. ..
  9. Fukuda M, Kobayashi H, Ishibashi K, Ohbayashi N. Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct Funct. 2011;36:155-70 pubmed
    ..Recombinant RBD35 was found to trap GTP-Rab35 specifically both in vitro and in PC12 cells, and overexpression of fluorescently tagged RBD35 in PC12 cells strongly inhibited nerve growth factor-dependent neurite outgrowth. ..
  10. Marubashi S, Shimada H, Fukuda M, Ohbayashi N. RUTBC1 Functions as a GTPase-activating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes. J Biol Chem. 2016;291:1427-40 pubmed publisher
    ..These results collectively indicate that proper spatiotemporal regulation of Rab32/38 is essential for the trafficking of all three melanogenic enzymes in mouse melanocytes. ..
  11. Fukuda M. Rab27 effectors, pleiotropic regulators in secretory pathways. Traffic. 2013;14:949-63 pubmed publisher
    ..g. exosome secretion and mast cell secretion) even within the same cell type. In this review article, I describe the current state of our understanding of the functions of Rab27 effectors in secretory pathways. ..