Yutaro Obara

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. ncbi request reprint Neurotrophic factor production in human astrocytoma cells by 2,5,6-tribromogramine via activation of epsilon isoform of protein kinase C
    Masaki Saito
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Eur J Pharm Sci 28:263-71. 2006
  2. ncbi request reprint The requirement of Ras and Rap1 for the activation of ERKs by cAMP, PACAP, and KCl in cerebellar granule cells
    Yutaro Obara
    Vollum Institute, L474, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurochem 101:470-82. 2007
  3. ncbi request reprint A novel cyathane diterpene, cyrneine A, induces neurite outgrowth in a Rac1-dependent mechanism in PC12 cells
    Yutaro Obara
    Department of Cellular Signaling and 21st COE program CRESCENDO, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Sendai, Miyagi, Japan
    Life Sci 80:1669-77. 2007
  4. ncbi request reprint 5,19-cyclo-9beta,10xi-androstane-3,17-dione promotes neurotrophic factor biosynthesis in 1321N1 human astrocytoma cells and improves passive avoidance learning impairment
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Sendai, Japan
    Brain Res 1184:57-64. 2007
  5. doi request reprint Betagamma subunits of G(i/o) suppress EGF-induced ERK5 phosphorylation, whereas ERK1/2 phosphorylation is enhanced
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Cell Signal 20:1275-83. 2008
  6. ncbi request reprint [Development of anti-dementia drugs related to neurotrophic factors]
    Yutaro Obara
    Department of Cellular Signaling and 21st Century COE Program CRESCENDO, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai City, Japan
    Yakugaku Zasshi 126:747-55. 2006
  7. pmc ERK5 activity is required for nerve growth factor-induced neurite outgrowth and stabilization of tyrosine hydroxylase in PC12 cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    J Biol Chem 284:23564-73. 2009
  8. doi request reprint The signaling pathway leading to extracellular signal-regulated kinase 5 (ERK5) activation via G-proteins and ERK5-dependent neurotrophic effects
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Pharmacol 77:10-6. 2010
  9. ncbi request reprint Thromboxane A2 promotes interleukin-6 biosynthesis mediated by an activation of cyclic AMP-response element-binding protein in 1321N1 human astrocytoma cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Pharmacol 68:670-9. 2005
  10. doi request reprint Basic fibroblast growth factor promotes glial cell-derived neurotrophic factor gene expression mediated by activation of ERK5 in rat C6 glioma cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, Japan
    Cell Signal 23:666-72. 2011

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Neurotrophic factor production in human astrocytoma cells by 2,5,6-tribromogramine via activation of epsilon isoform of protein kinase C
    Masaki Saito
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Eur J Pharm Sci 28:263-71. 2006
    ..These results indicate that 2,5,6-tribromogramine promotes the synthesis and secretion of neurotrophic factors including NGF in 1321N1 cells via an activation of PKC-epsilon...
  2. ncbi request reprint The requirement of Ras and Rap1 for the activation of ERKs by cAMP, PACAP, and KCl in cerebellar granule cells
    Yutaro Obara
    Vollum Institute, L474, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurochem 101:470-82. 2007
    ..Furthermore, Rap1, but not Ras, activation by PKA appears to require the action of Src family kinases...
  3. ncbi request reprint A novel cyathane diterpene, cyrneine A, induces neurite outgrowth in a Rac1-dependent mechanism in PC12 cells
    Yutaro Obara
    Department of Cellular Signaling and 21st COE program CRESCENDO, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Sendai, Miyagi, Japan
    Life Sci 80:1669-77. 2007
    ..These results suggest that cyrneine A induces neurite outgrowth in a Rac1-dependent mechanism...
  4. ncbi request reprint 5,19-cyclo-9beta,10xi-androstane-3,17-dione promotes neurotrophic factor biosynthesis in 1321N1 human astrocytoma cells and improves passive avoidance learning impairment
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Sendai, Japan
    Brain Res 1184:57-64. 2007
    ..Taking these results together, CAD has enormous therapeutic potential for serious neuronal diseases...
  5. doi request reprint Betagamma subunits of G(i/o) suppress EGF-induced ERK5 phosphorylation, whereas ERK1/2 phosphorylation is enhanced
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Cell Signal 20:1275-83. 2008
    ..In response to LPA, Gbetagamma subunits interacted with EGF receptor in a time-dependent manner. These results strongly suggest that LPA negatively regulates the EGF-induced ERK5 phosphorylation through Gbetagamma subunits...
  6. ncbi request reprint [Development of anti-dementia drugs related to neurotrophic factors]
    Yutaro Obara
    Department of Cellular Signaling and 21st Century COE Program CRESCENDO, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai City, Japan
    Yakugaku Zasshi 126:747-55. 2006
    ..In addition, we discuss about the signaling pathway of nerve growth factor and cyclic AMP in order to seek the signaling molecules as targets of drug development...
  7. pmc ERK5 activity is required for nerve growth factor-induced neurite outgrowth and stabilization of tyrosine hydroxylase in PC12 cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    J Biol Chem 284:23564-73. 2009
    ..Taking these results together, ERK5 is involved in neurite outgrowth and stabilization of tyrosine hydroxylase in PC12 cells, and ERK5, along with ERK1/2, plays essential roles in the neural differentiation process...
  8. doi request reprint The signaling pathway leading to extracellular signal-regulated kinase 5 (ERK5) activation via G-proteins and ERK5-dependent neurotrophic effects
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Pharmacol 77:10-6. 2010
    ..In this review, the signaling pathway leading to ERK5 activation through heterotrimeric and small G-proteins and the physiological roles of ERK5 in neuronal cells are summarized and discussed...
  9. ncbi request reprint Thromboxane A2 promotes interleukin-6 biosynthesis mediated by an activation of cyclic AMP-response element-binding protein in 1321N1 human astrocytoma cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Mol Pharmacol 68:670-9. 2005
    ..In conclusion, TXA2 enhances the IL-6 biosynthesis via the PKA p38 MAPK/CREB pathway in 1321N1 cells. IL-6 induction depends on Galpha(q) and Galpha13 as well. This is the first report showing TP-mediated IL-6 production in glial cells...
  10. doi request reprint Basic fibroblast growth factor promotes glial cell-derived neurotrophic factor gene expression mediated by activation of ERK5 in rat C6 glioma cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai, Japan
    Cell Signal 23:666-72. 2011
    ..Taking these results together, bFGF promotes GDNF expression accompanied by the activation of ERK5, ERK1/2 and their downstream transcription factors in C6 glioma cells...
  11. pmc Lysophosphatidylinositol causes neurite retraction via GPR55, G13 and RhoA in PC12 cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    PLoS ONE 6:e24284. 2011
    ..These results suggest that LPI, and not cannabinoids, causes neurite retraction in differentiated PC12 cells via a GPR55, G(13) and RhoA signaling pathway...
  12. doi request reprint Inhibitory effect of hericenone B from Hericium erinaceus on collagen-induced platelet aggregation
    Koichiro Mori
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, 6 3 Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Phytomedicine 17:1082-5. 2010
    ..Moreover, we found that collagen-induced aggregation was inhibited by hericenone B in human platelets, similar to in rabbit platelets...
  13. doi request reprint P2Y2 receptor-Gq/11 signaling at lipid rafts is required for UTP-induced cell migration in NG 108-15 cells
    Koji Ando
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
    J Pharmacol Exp Ther 334:809-19. 2010
    ..These results suggest that lipid rafts are critical for P2Y(2) receptor-mediated G(q/11)-PLC-Ca(2+) signaling and this cascade is important for cell migration in NG 108-15 cells...
  14. ncbi request reprint Complanadines C and D, new dimeric alkaloids from Lycopodium complanatum
    Takaaki Kubota
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Bioorg Med Chem 15:413-7. 2007
    ..Complanadine D (2) enhanced mRNA expression for NGF...
  15. ncbi request reprint Nerve growth factor-inducing activity of Hericium erinaceus in 1321N1 human astrocytoma cells
    Koichiro Mori
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba ku, Sendai, Japan
    Biol Pharm Bull 31:1727-32. 2008
    ..In conclusion, H. erinaceus contains active compounds that stimulate NGF synthesis via activation of the JNK pathway; these compounds are not hericenones...
  16. ncbi request reprint Lycopladines B-D and lyconadin B, new alkaloids from Lycopodium complanatum
    Kan ichiro Ishiuchi
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Bioorg Med Chem 14:5995-6000. 2006
    ..Lyconadin B (4) elevated NGF mRNA expression in 1321N1 human astrocytoma cells...
  17. doi request reprint Gα(h)/transglutaminase-2 activity is required for maximal activation of adenylylcyclase 8 in human and rat glioma cells
    Yutaro Obara
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Cell Signal 25:589-97. 2013
    ..Taking these results together, Gα(h)/TG2 promotes cAMP production accompanied by a modification of adenylylcyclase 8 in glioma cells...
  18. doi request reprint Functional interaction between purinergic receptors: effect of ligands for A2A and P2Y12 receptors on P2Y1 receptor function
    Tokiko Suzuki
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba ku, Sendai, Japan
    FEBS Lett 585:3978-84. 2011
    ..We expect that intermolecular signal transduction and specific conformational changes occur among components of hetero-oligomers formed by these three receptors...
  19. doi request reprint A synthetic cannabinoid, CP55940, inhibits lipopolysaccharide-induced cytokine mRNA expression in a cannabinoid receptor-independent mechanism in rat cerebellar granule cells
    Toshiki Chiba
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba ku, Sendai, Japan
    J Pharm Pharmacol 63:636-47. 2011
    ..In this study, we tried to elucidate possible effects of cannabinoids on lipopolysaccharide (LPS)-induced expression of inflammatory cytokine mRNAs in rat cerebellar granule cells...
  20. doi request reprint Involvement of lipid rafts in multiple signal transductions mediated by two isoforms of thromboxane A₂ receptor: dependency on receptor isoforms and downstream signaling types
    Shinji Goto
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6 3, Aramaki, Aoba ku, Sendai 980 8578, Japan
    Eur J Pharmacol 693:15-24. 2012
    ..Moreover, our results provide a deeper understanding of the function of lipid rafts in G protein signaling and the physiological meaning of TP isoforms...
  21. ncbi request reprint Beta-eudesmol induces neurite outgrowth in rat pheochromocytoma cells accompanied by an activation of mitogen-activated protein kinase
    Yutaro Obara
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba ku, Sendai 980 8578, Japan
    J Pharmacol Exp Ther 301:803-11. 2002
    ..Beta-eudesmol, being a small molecule, may therefore be a promising lead compound for potentiating neuronal function. Furthermore, the drug may be useful in helping to clarify the mechanisms underlying neuronal differentiation...
  22. ncbi request reprint Effects of Hericium erinaceus on amyloid β(25-35) peptide-induced learning and memory deficits in mice
    Koichiro Mori
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Japan
    Biomed Res 32:67-72. 2011
    ..erinaceus prevented impairments of spatial short-term and visual recognition memory induced by amyloid β(25-35) peptide. This finding indicates that H. erinaceus may be useful in the prevention of cognitive dysfunction...
  23. doi request reprint Thromboxane A2 receptor-mediated epidermal growth factor receptor transactivation: involvement of PKC-delta and PKC-epsilon in the shedding of epidermal growth factor receptor ligands
    Kotomi Uchiyama
    Department of Cellular Signaling, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Eur J Pharm Sci 38:504-11. 2009
    ....
  24. ncbi request reprint Theonezolide A, a novel marine macrolide, induces drastic shape change in rabbit platelets by reorganization of microtubules
    Minori Mitsui-Saito
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Thromb Res 108:133-8. 2002
    ..These results suggest that theonezolide A induces drastic shape change through reorganization of microtubules in rabbit platelets. Thus, theonezolide A is a useful drug to examine microtubule reorganization in the cells...
  25. ncbi request reprint Daphmanidins C and D, novel pentacyclic alkaloids from Daphniphyllum teijsmanii
    Hiroshi Morita
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Org Lett 7:459-62. 2005
    ..The relative stereochemistry of 1 and 2 was assigned by combination of NOESY correlations and a simulation analysis. Daphmanidin C (1) elevated activity of NGF biosynthesis...
  26. ncbi request reprint Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages
    Syu ichi Kanno
    Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Aoba ku, Sendai 981 8558, Japan
    Life Sci 78:673-81. 2006
    ..These findings suggest that suppression of the LPS-induced mortality and production of NO by NG is due to inhibition of the activation of NF-kappaB...
  27. ncbi request reprint Tricholomalides A-C, new neurotrophic diterpenes from the mushroom Tricholoma sp
    Sachiko Tsukamoto
    Faculty of Pharmaceutical Sciences, Kanazawa University, 13 1 Takara machi, Kanazawa 920 0934, Japan
    J Nat Prod 66:1578-81. 2003
    ..Tricholomalides 1-3 significantly induced neurite outgrowth in rat pheochromocytoma cells (PC-12) at concentrations of 100 microM...
  28. ncbi request reprint PKA phosphorylation of Src mediates Rap1 activation in NGF and cAMP signaling in PC12 cells
    Yutaro Obara
    The Vollum Institute, L474, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    J Cell Sci 117:6085-94. 2004
    ..PKA phosphorylation of Src may therefore be required for Rap1 activation in PC12 cells...
  29. ncbi request reprint Neurite outgrowth activity of cyathane diterpenes from Sarcodon cyrneus, cyrneines A and B
    Maria Carla Marcotullio
    Dipartimento di Chimica e Tecnologia del Farmaco, Sez Chimica Organica, Universita degli Studi, Perugia, Italy
    Planta Med 72:819-23. 2006
    ..Interestingly, cyrneines A and B significantly promoted neurite outgrowth in addition to NGF as a positive control...
  30. ncbi request reprint Cyathane diterpenes from Sarcodon cyrneus and evaluation of their activities of neuritegenesis and nerve growth factor production
    Maria Carla Marcotullio
    Dipartimento di Chimica e Tecnologia del Farmaco Sez Chimica Organica, Universita degli Studi, 06123 Perugia, Italy
    Bioorg Med Chem 15:2878-82. 2007
    ..The structures of the novel diterpenoids were determined by the analysis of spectroscopic data. Effects of the cyrneines and glaucopine C on the NGF gene expression in 1321N1 cells and on neurite outgrowth on PC12 cells were evaluated...