Kensaku Mizuno

Summary

Affiliation: Tohoku University
Country: Japan

Publications

  1. pmc Spatial and temporal regulation of cofilin activity by LIM kinase and Slingshot is critical for directional cell migration
    Michiru Nishita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Cell Biol 171:349-59. 2005
  2. pmc Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells
    Takuji Tsuji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Mol Biol Cell 21:3590-600. 2010
  3. pmc Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers
    Tai Kiuchi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    J Cell Biol 177:465-76. 2007
  4. pmc A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia
    Kyoko Nagata-Ohashi
    Dept of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Cell Biol 165:465-71. 2004
  5. doi request reprint Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation
    Kensaku Mizuno
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Cell Signal 25:457-69. 2013
  6. ncbi request reprint Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin
    Yusaku Ohta
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Genes Cells 8:811-24. 2003
  7. ncbi request reprint Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing
    Go Tomiyoshi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Genes Cells 9:591-600. 2004
  8. ncbi request reprint Cell cycle-associated changes in Slingshot phosphatase activity and roles in cytokinesis in animal cells
    Noriko Kaji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 278:33450-5. 2003
  9. doi request reprint Molecular dissection of the mechanisms of substrate recognition and F-actin-mediated activation of cofilin-phosphatase Slingshot-1
    Souichi Kurita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 283:32542-52. 2008
  10. ncbi request reprint Phosphoinositide 3-kinase-mediated activation of cofilin phosphatase Slingshot and its role for insulin-induced membrane protrusion
    Michiru Nishita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, USA
    J Biol Chem 279:7193-8. 2004

Collaborators

Detail Information

Publications39

  1. pmc Spatial and temporal regulation of cofilin activity by LIM kinase and Slingshot is critical for directional cell migration
    Michiru Nishita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Cell Biol 171:349-59. 2005
    ..We propose that LIMK1- and SSH1L-mediated spatiotemporal regulation of cofilin activity is critical for chemokine-induced polarized lamellipodium formation and directional cell movement...
  2. pmc Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells
    Takuji Tsuji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Mol Biol Cell 21:3590-600. 2010
    ..Our results suggest that p114-RhoGEF and Lfc are critically involved in Wnt-3a- and Dvl-induced RhoA activation and neurite retraction in N1E-115 cells...
  3. pmc Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers
    Tai Kiuchi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    J Cell Biol 177:465-76. 2007
    ..We propose that cofilin contributes to stimulus-induced actin filament assembly and lamellipodium extension by supplying an abundant pool of cytoplasmic actin monomers...
  4. pmc A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia
    Kyoko Nagata-Ohashi
    Dept of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Cell Biol 165:465-71. 2004
    ....
  5. doi request reprint Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation
    Kensaku Mizuno
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Cell Signal 25:457-69. 2013
    ..Perturbations in the normal control of cofilin/ADF activity underlie many pathological conditions, including cancer metastasis and neurological and cardiovascular disorders...
  6. ncbi request reprint Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin
    Yusaku Ohta
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Genes Cells 8:811-24. 2003
    ..Cofilin, a key regulator of actin filament dynamics, is inactivated by phosphorylation at Ser-3 by LIM-kinases and is reactivated by dephosphorylation by a family of protein phosphatases, termed Slingshot (SSH)...
  7. ncbi request reprint Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing
    Go Tomiyoshi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Genes Cells 9:591-600. 2004
    ..These findings suggest that caspase-mediated cleavage and activation of LIMK1 play a role in the membrane bleb formation during apoptosis...
  8. ncbi request reprint Cell cycle-associated changes in Slingshot phosphatase activity and roles in cytokinesis in animal cells
    Noriko Kaji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 278:33450-5. 2003
    ..These results suggest that SSH1 plays a critical role in cytokinesis by dephosphorylating and reactivating cofilin in later stages of mitosis...
  9. doi request reprint Molecular dissection of the mechanisms of substrate recognition and F-actin-mediated activation of cofilin-phosphatase Slingshot-1
    Souichi Kurita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 283:32542-52. 2008
    ..Together, these findings suggest that the conserved SSH-N domain plays critical roles in P-cofilin recognition, F-actin-mediated activation, and subcellular localization of SSH1...
  10. ncbi request reprint Phosphoinositide 3-kinase-mediated activation of cofilin phosphatase Slingshot and its role for insulin-induced membrane protrusion
    Michiru Nishita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, USA
    J Biol Chem 279:7193-8. 2004
    ..These findings suggest that SSH1L is activated downstream of PI3K and plays a critical role in insulin-induced membrane protrusion by dephosphorylating and activating cofilin...
  11. ncbi request reprint LIM kinase and slingshot are critical for neurite extension
    Mitsuharu Endo
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 282:13692-702. 2007
    ..These results suggest that proper regulation of cofilin/ADF activities through control of phosphorylation by LIMKs and SSHs is critical for neurite extension and that LIMKs regulate actin filament assembly at the tip of the growth cone...
  12. ncbi request reprint Control of growth cone motility and morphology by LIM kinase and Slingshot via phosphorylation and dephosphorylation of cofilin
    Mitsuharu Endo
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980 8578, Japan
    J Neurosci 23:2527-37. 2003
    ..Using various mutants of cofilin, we also obtained evidence that the actin-filament-severing activity of cofilin is critical for growth cone motility and neurite extension...
  13. pmc MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration
    Miho Kobayashi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    EMBO J 25:713-26. 2006
    ..These findings suggest that MK2-mediated LIMK1 phosphorylation/activation plays an essential role in VEGF-A-induced actin reorganization, migration, and tubule formation of endothelial cells...
  14. pmc Ca2+/calmodulin-dependent protein kinase IV-mediated LIM kinase activation is critical for calcium signal-induced neurite outgrowth
    Miyohiko Takemura
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 284:28554-62. 2009
    ..Taken together, our results suggest that LIMK1-mediated cofilin phosphorylation is critical for ionomycin-induced neurite outgrowth and that CaMKIV mediates ionomycin-induced LIMK1 activation...
  15. doi request reprint Critical roles of actin-interacting protein 1 in cytokinesis and chemotactic migration of mammalian cells
    Asuka Kato
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem J 414:261-70. 2008
    ..This indicates that Aip1 plays an important role in directional cell migration by restricting the stimulus-induced membrane protrusion to one direction via promoting cofilin activity...
  16. doi request reprint Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM kinase
    Yuji Horita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    J Biol Chem 283:6013-21. 2008
    ....
  17. doi request reprint Coactosin accelerates cell dynamism by promoting actin polymerization
    Xubin Hou
    Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Japan
    Dev Biol 379:53-63. 2013
    ..From these results we have concluded that Coactosin functions downstream of Rac signaling and that it is involved in neurite extension and neural crest cell migration by actively participating in actin polymerization...
  18. ncbi request reprint Calcium signal-induced cofilin dephosphorylation is mediated by Slingshot via calcineurin
    Yan Wang
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 280:12683-9. 2005
    ..Based on these findings, we suggest that Ca2+-induced cofilin dephosphorylation is mediated by calcineurin-dependent activation of SSH1L...
  19. ncbi request reprint Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L
    Masahiro Yamamoto
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    FEBS Lett 580:1789-94. 2006
    ..These sites play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L...
  20. doi request reprint Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments
    Kazumasa Ohashi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Biotechniques 52:45-50. 2012
    ....
  21. pmc Furry protein promotes aurora A-mediated Polo-like kinase 1 activation
    Masanori Ikeda
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 287:27670-81. 2012
    ..Our results suggest that Fry plays a crucial role in the structural integrity of mitotic centrosomes and in the maintenance of spindle bipolarity by promoting Plk1 activity at the spindle poles in early mitosis...
  22. pmc Stromal cell-derived factor 1alpha activates LIM kinase 1 and induces cofilin phosphorylation for T-cell chemotaxis
    Michiru Nishita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980 8578, Japan
    Mol Cell Biol 22:774-83. 2002
    ..These results suggest that the phosphorylation of cofilin by LIMK1 plays a critical role in the SDF-1alpha-induced chemotactic response of T lymphocytes...
  23. pmc CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment
    Go Itoh
    Institute of Development, Aging and Cancer, Tohoku University, Miyagi, Japan
    EMBO J 30:130-44. 2011
    ..These data suggest that CAMP is required for maintaining kinetochore-microtubule attachment during bi-orientation...
  24. pmc LIM kinase has a dual role in regulating lamellipodium extension by decelerating the rate of actin retrograde flow and the rate of actin polymerization
    Kazumasa Ohashi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 286:36340-51. 2011
    ....
  25. ncbi request reprint LIM kinase-mediated cofilin phosphorylation during mitosis is required for precise spindle positioning
    Noriko Kaji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 283:4983-92. 2008
    ..Our results suggest that LIMK1-mediated cofilin phosphorylation is required for accurate spindle orientation by stabilizing cortical actin networks during mitosis...
  26. doi request reprint p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells
    Aya Hayashi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    FEBS Lett 587:698-705. 2013
    ..These results indicate that p63RhoGEF plays a crucial role in serum-induced chemotaxis by limiting lamellipodial protrusion to one direction via RhoA activation...
  27. ncbi request reprint Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-1
    Souichi Kurita
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Genes Cells 12:663-76. 2007
    ..SSH1 thus has the potential to regulate actin filament dynamics and organization in cells via F-actin-stabilizing and -bundling activities, in addition to its ability to dephosphorylate cofilin...
  28. ncbi request reprint Furry promotes acetylation of microtubules in the mitotic spindle by inhibition of SIRT2 tubulin deacetylase
    Tomoaki Nagai
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Cell Sci 126:4369-80. 2013
    ..In summary, these results suggest that Fry plays a crucial role in promoting the level of MT acetylation in the mitotic spindle by inhibiting the tubulin-deacetylase activity of SIRT2...
  29. doi request reprint MST2- and Furry-mediated activation of NDR1 kinase is critical for precise alignment of mitotic chromosomes
    Shuhei Chiba
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan
    Curr Biol 19:675-81. 2009
    ..Our findings suggest that MST2-, Fry-, and MOB2-mediated activation of NDR1 is crucial for the fidelity of mitotic chromosome alignment in mammalian cells...
  30. doi request reprint LIM-kinase is critical for the mesenchymal-to-amoeboid cell morphological transition in 3D matrices
    Toshiaki Mishima
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem Biophys Res Commun 392:577-81. 2010
    ..Furthermore, LIMK1 knockdown suppressed the invasive activity of HT1080 cells in collagen gels with or without PIs, indicating that LIMK1 mediates both the mesenchymal and amoeboid modes of invasion of HT1080 cells...
  31. ncbi request reprint Mitosis-specific activation of LIM motif-containing protein kinase and roles of cofilin phosphorylation and dephosphorylation in mitosis
    Toru Amano
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miagi 980 8578, Japan
    J Biol Chem 277:22093-102. 2002
    ....
  32. doi request reprint Cytochalasin D acts as an inhibitor of the actin-cofilin interaction
    Kazuyasu Shoji
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem Biophys Res Commun 424:52-7. 2012
    ..These results suggest that CytoD inhibits actin filament dynamics in cells via multiple mechanisms, including the well-known barbed-end capping mechanism and as shown in this study, the inhibition of G- and F-actin binding to cofilin...
  33. pmc NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity from phosphatidylserine to Sec15
    Shuhei Chiba
    Laboratory of Molecular Cell Biology, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    EMBO J 32:874-85. 2013
    ....
  34. pmc Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension
    Tai Kiuchi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    J Cell Biol 193:365-80. 2011
    ....
  35. pmc Damnacanthal, an effective inhibitor of LIM-kinase, inhibits cell migration and invasion
    Kazumasa Ohashi
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980 8574, Japan Graduate School of Nanobioscience, Yokohama City University, Seto, Kanazawa Ku, Yokohama 236 0027, Japan
    Mol Biol Cell 25:828-40. 2014
    ..The bimolecular fluorescence complementation assay system used in this study will provide a useful method to screen for inhibitors of various protein kinases. ..
  36. doi request reprint CaMKII╬▓-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis
    Akihiko Saito
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980 8578, Japan
    Genes Cells 18:533-43. 2013
    ..In summary, these results suggest that CaMKII╬▓-mediated activation of LIMK1 plays a crucial role in BDNF-induced enhancement of primary neurite formation...
  37. pmc Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase
    Yoshikazu Tsumura
    Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi 980 8578, Japan
    Biochem J 387:627-37. 2005
    ....
  38. ncbi request reprint A novel pair of split venus fragments to detect protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays
    Kazumasa Ohashi
    Graduate School of Life Sciences, Tohoku University, Aramaki Aza Aoba, Aoba ku, Sendai, Miyagi, 980 8578, Japan
    Methods Mol Biol 1174:247-62. 2014
    ..Moreover, these assays can be applied to screen small-molecule inhibitors of protein-protein interactions. ..
  39. ncbi request reprint Multifaceted roles of Furry proteins in invertebrates and vertebrates
    Tomoaki Nagai
    Laboratory of Molecular Cell Biology, Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Biochem 155:137-46. 2014
    ..Here, we review current knowledge about the diverse cellular functions and regulation of Fry proteins in invertebrates and vertebrates. ..