Research Topics
Genomes and Genes | Haruki KitazawaSummaryAffiliation: Tohoku University Country: Japan Publications
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Publications
A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilus group to human colonic mucinHideaki Uchida
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Biosci Biotechnol Biochem 68:1004-10. 2004..The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM...
Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammationNaoya Takanashi
Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Aoba ku, 981 8555, Japan
BMC Microbiol 13:54. 2013....- Phosphate group requirement for mitogenic activation of lymphocytes by an extracellular phosphopolysaccharide from Lactobacillus delbrueckii ssp. bulgaricusH Kitazawa
Laboratory of Animal Products Chemistry, Graduate School of Agriculture, Tohoku University, Sendai, Japan
Int J Food Microbiol 40:169-75. 1998..The phosphopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus 1073R-1 is a potent B-cell-dependent mitogen in which the phosphate group acts as a trigger of the mitogenic induction... - AT oligonucleotides inducing B lymphocyte activation exist in probiotic Lactobacillus gasseriH Kitazawa
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Int J Food Microbiol 65:149-62. 2001..gasseri and proposed that they have potential as applicants for the production of new functional foods, "Bio-Defense Foods"... - A novel immunostimulating aspect of Lactobacillus gasseri: induction of "Gasserokine" as chemoattractants for macrophagesHaruki Kitazawa
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Int J Food Microbiol 77:29-38. 2002..Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria... - Immunostimulatory oligonucleotide, CpG-like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAI B6Haruki Kitazawa
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aobaku, 981 8555, Sendai, Japan
Int J Food Microbiol 85:11-21. 2003..This study demonstrated that L. bulgaricus NIAI B6 was a good candidate of a starter culture for the production of new functional foods, "Bio-Defense Foods"... - Enzymatic digestion of the milk protein beta-casein releases potent chemotactic peptide(s) for monocytes and macrophagesHaruki Kitazawa
Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute at Frederick, National Institutes of Health, Frederick, USA
Int Immunopharmacol 7:1150-9. 2007..Our study suggests that biologically active peptides released by actinase-digested milk beta-casein may promote innate host immune responses by inducing macrophage migration and activation... - Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cellsShoichi Hosoya
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Vet Res 42:111. 2011..casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response... 
A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattleEriko Chiba
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Res Vet Sci 93:688-94. 2012....- Strong immunostimulatory activity of AT-oligodeoxynucleotide requires a six-base loop with a self-stabilized 5'-C...G-3' stem structureTakeshi Shimosato
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
Cell Microbiol 8:485-95. 2006..Finally, we found that novel strong immunostimulatory non-CpG AT-ODNs exist in the genome of probiotic lactic acid bacteria... - Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathwayTomoyuki Shimazu
Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Infect Immun 80:276-88. 2012.... - Toll-like receptor 2 is expressed on the intestinal M cells in swineMasanori Tohno
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Biochem Biophys Res Commun 330:547-54. 2005..Furthermore, the potential function of TLR2 as a pattern recognition receptor and its cellular distribution suggest that TLR2 plays an important role in ligand-specific transcytosis and transport in M cells... - Cytokeratin 18 is a specific marker of bovine intestinal M cellTetsuya Hondo
Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku Univ, Sendai, Miyag, Japan
Am J Physiol Gastrointest Liver Physiol 300:G442-53. 2011..These data indicate that CK18 may be a molecular marker for bovine M cells in FAE and that M cells may transdifferentiate to CK20-positive enterocytes and die by apoptosis in the apex of the FAE... - An adhesin-like protein, Lam29, from Lactobacillus mucosae ME-340 binds to histone H3 and blood group antigens in human colonic mucusMasamichi Watanabe
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981 8555, Japan
Biosci Biotechnol Biochem 76:1655-60. 2012..This is the first report to indicate that lactobacilli expressing Lam29 adhere to histone H3 on gastrointestinal mucosa... - Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: finding new anti-inflammatory immunobioticsHitomi Fujie
Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
FEMS Immunol Med Microbiol 63:129-39. 2011..Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation... - Purification and characterization of two phospho-β-galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323(T)Hiroyuki Honda
Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
J Gen Appl Microbiol 58:11-7. 2012..Our data suggest two phospho-β-galactosidase, LacG1 and LacG2, are the primary enzymes for lactose utilization in Lb. gasseri ATCC33323(T). We propose a reclassification of Pbg1 and Pbg2 as phospho-β-glucosidase... - Transcytosis of murine-adapted bovine spongiform encephalopathy agents in an in vitro bovine M cell modelKohtaro Miyazawa
Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai, Japan
J Virol 84:12285-91. 2010.... - Characterization of newly established bovine intestinal epithelial cell lineKohtaro Miyazawa
Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai, Japan
Histochem Cell Biol 133:125-34. 2010..We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells... - Immunobiotic Lactobacillus jensenii modulates the Toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen-presenting cellsJulio Villena
Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Clin Vaccine Immunol 19:1038-53. 2012.... - Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3Yasuhiro Nagai
Laboratory of Functional Morphology, Tohoku University, Sendai, Japan
J Immunol 181:6073-81. 2008..These results suggest that an established BAPC-1 may originate from these progenitor cells, and that the progenitor cells with 4Ig-B7-H3 may play a critical role in the immunoendocrine network... - Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4Wataru Ueda
Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Vet Immunol Immunopathol 143:148-54. 2011..These results should help contribute to the understanding of PGLYRP3- and 4-mediated immune responses via their recognition of intestinal microorganisms in newborn and adult swine... - Molecular cloning and functional characterization of porcine nucleotide-binding oligomerization domain-2 (NOD2)Masanori Tohno
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Mol Immunol 45:194-203. 2008..Our results implicate NOD2 as an important immunoregulator in the swine intestinal immunity... - Lactic acid bacteria (LAB) bind to human B- or H-antigens expressed on intestinal mucosaHideaki Uchida
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
Biosci Biotechnol Biochem 70:3073-6. 2006..We found two strains, L. gasseri OLL2755 and L. gasseri OLL2877 that firmly adhere to human B-antigen, and we found L. gasseri OLL2827 bound to the H-antigen... - Toll-like receptor 2 and 9 are expressed and functional in gut-associated lymphoid tissues of presuckling newborn swineMasanori Tohno
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai, 981-8555, Japan
Vet Res 37:791-812. 2006..These results indicate that TLR2 and TLR9 allow MLN and ileal Pps to respond to a variety of bacterial components immediately after birth, thereby providing newborns with a host defense system... - Molecular cloning of porcine RP105/MD-1 involved in recognition of extracellular phosphopolysaccharides from Lactococcus lactis ssp. cremorisMasanori Tohno
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Mol Immunol 44:2566-77. 2007..These effects were mediated by phosphatidylinositol 3-kinase and Bruton's tyrosine kinase. Thus, we identified extracellular polysaccharide from Lc.cremoris as an active substance that can induce immune activation via RP105 and MD-1... - Augmentation of T(H)-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signalingTakeshi Shimosato
Graduate School of Agricultural Science, Tohoku University, Tsutsumidori Amamiyamachi 1 1, Aoba ku, Sendai shi 981 8555, Japan
Biochem Biophys Res Commun 326:782-7. 2005..Moreover, the swine TLR9 transfectant demonstrated its usefulness for evaluation of immunostimulation by bacterial DNA through the detection of T(H)-1, T(H)-2 type cytokine induction via TLR9 signaling... - Lactobacilli binding human A-antigen expressed in intestinal mucosaHideaki Uchida
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori Amamiyamachi 1 1, Aoba ku, Sendai 981 8555, Miyagi, Japan
Res Microbiol 157:659-65. 2006..brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut... - Utilization of the porcine system to study lymphotoxin-beta regulation in intestinal lymphoid tissueTomoyuki Shimazu
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai, 981 8555, Japan
Biochem Genet 47:126-36. 2009..LT-beta may therefore be useful for studying the development of the intestinal immune system at parturition in both swine and humans... - Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineageTakashi Kanaya
Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku Univ, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, 981 8555 Sendai, Japan
Am J Physiol Gastrointest Liver Physiol 295:G273-84. 2008..We report here that MIE cells have the potential ability to differentiate into M cells and that this differentiation required activated T cells and B cells... - Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigensHideki Kinoshita
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori Amamiyamachi 1 1, Aoba ku, Sendai, Miyagi 981 8555, Japan
Res Microbiol 159:685-91. 2008..The binding of GAPDH to HCM significantly decreased after incubation with NAD+. This suggests that the NAD binding domain on GAPDH may be related to binding to HCM... - Toll-like receptor 9 is expressed on follicle-associated epithelia containing M cells in swine Peyer's patchesTakeshi Shimosato
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Japan
Immunol Lett 98:83-9. 2005..These results suggest that Pps and MLNs provide the host defense with the ability to respond to a variety of bioactive oligonucleotides (ODNs) from bacteria at a conductive site of initial immune responses... - Molecular cloning and functional characterization of porcine MyD88 essential for TLR signalingMasanori Tohno
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Cell Mol Immunol 4:369-76. 2007..These results suggest that an MyD88-dependent signaling pathway is present in newborn as well as in adult swine and that it is involved in the innate immune system of these animals... - Staining patterns for actin and villin distinguish M cells in bovine follicle-associated epitheliumTakashi Kanaya
Laboratory of Functional Morphology, Tohoku University, 1 1 Tsutsumidori, Amamiyamachi, Aoba ku, 981 8555 Sendai, Japan
Res Vet Sci 82:141-9. 2007..As the microvilli of M cells were very sparse, there were several areas of weak immunostaining in calf jejunal FAE. These results suggest that M cells in calf FAE are detectable by the absence of staining for actin and villin... - DNA sequencing and homologous expression of a small peptide conferring immunity to gassericin A, a circular bacteriocin produced by Lactobacillus gasseri LA39Yasushi Kawai
Laboratory of Animal Products Chemistry, Biological Resource Science, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai, Japan
Appl Environ Microbiol 75:1324-30. 2009..Comparison of GaaI to peptides with similar characteristics found in the circular bacteriocin gene loci is discussed... - Immunoregulatory Effect of Bifidobacteria Strains in Porcine Intestinal Epithelial Cells through Modulation of Ubiquitin-Editing Enzyme A20 ExpressionYohsuke Tomosada
Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
PLoS ONE 8:e59259. 2013..We previously showed that evaluation of anti-inflammatory activities of lactic acid bacteria in porcine intestinal epithelial (PIE) cells is useful for selecting potentially immunobiotic strains... - Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell lineMasayuki Moue
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Biochim Biophys Acta 1780:134-44. 2008.... - A swine toll-like receptor 2-expressing transfectant as a potential primary screening system for immunobiotic microorganismsMasanori Tohno
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aobaku, Sendai 981 8555, Japan
FEMS Immunol Med Microbiol 44:283-8. 2005..These findings suggest that the swine TLR2-expressing transfectant can be very useful for the primary screening of immunobiotic microorganisms... - Strong immunostimulation in murine immune cells by Lactobacillus rhamnosus GG DNA containing novel oligodeoxynucleotide patternIliyan D Iliev
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
Cell Microbiol 7:403-14. 2005..In this study we demonstrate for a first time that, DNA from LGG is a factor of immunobiotic activity. Furthermore, ODN ID35 is the first ODN, with such a strong immunostimulatory activity to be found in immunobiotic bacterial DNA... - Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and miceKohtaro Miyazawa
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Sendai 981 8555, Japan
Histochem Cell Biol 127:291-301. 2007..In this study, we demonstrate that there are a number of differences in the localization of PrP(c) between the murine and bovine small intestines... - Molecular cloning and functional characterization of porcine nucleotide-binding oligomerization domain-1 (NOD1) recognizing minimum agonists, meso-diaminopimelic acid and meso-lanthionineMasanori Tohno
Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
Mol Immunol 45:1807-17. 2008..Our results should help clarify how the intestinal immune system is modulated by low-molecular weight peptidoglycan fragments through NOD1... - Proposal of screening method for intestinal mucus adhesive lactobacilli using the enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH)Hideki Kinoshita
Department of Food Management, School of Food, Agricultural and Environmental Sciences, Miyagi University, Miyagi, Japan
Anim Sci J 84:150-8. 2013..01). The new screening method using GAPDH enzymatic activity without an adhesion test may be possible due to the significant positive correlation of GAPDH activity with adhesion of lactobacilli derived from the human intestine... - Cloning and characterization of Swine interleukin-17, preferentially expressed in the intestinesShinichiro Katoh
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aobaku, Sendai 981 8555, Japan
J Interferon Cytokine Res 24:553-9. 2004..The gene sequence and biologically active recombinat protein for PoIL-17 will be useful for elucidation of the role of IL-17 in the regulation of intestinal immune responses... - Apoptotic process of porcine intestinal M cellsKohtaro Miyazawa
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan
Cell Tissue Res 323:425-32. 2006..These data suggest that the committed M cells differentiate to mature M cells by contact with lymphocytes at the FAE periphery, and that they trans-differentiate to enterocytes and are finally excluded near the FAE apex... - Expression of myostatin in neural cells of the olfactory systemShunsuke Iwasaki
Laboratory of Functional Morphology, Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555, Japan
Mol Neurobiol 47:1-8. 2013..These findings suggest that myostatin is localized in specific cells associated with the olfactory system of the brain and may act as a key inhibitor in cell and/or signal development of the olfactory system... - Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary glandYasuhiro Nagai
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, 981 8555, Sendai, Japan
Cell Tissue Res 322:455-62. 2005..These data suggest that IL-18 acts on somatotrophs as an immuno-endocrine mediator through the autocrine pathway... - Identification of five phospho-beta-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose mediumSeiji Nagaoka
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan
Biosci Biotechnol Biochem 72:1954-7. 2008..To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose... - Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39Kiyoshi Nakamura
Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai, Japan
Anim Sci J 84:144-9. 2013..5% (w/w) glycine. This is the first highly practical application of GA to foods as a biopreservative, and the concentration method and the bacteriocin concentrate would contribute to biopreservation of several foods... - Swine Toll-like receptor 9(1) recognizes CpG motifs of human cell stimulantTakeshi Shimosato
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aobaku, 981 8555, Sendai, Japan
Biochim Biophys Acta 1627:56-61. 2003..The strong expression of sTLR9 in Pps and MLNs and its recognizing CpG DNA for human cell stimulant are shown first in this study, which may help in understanding the intestinal immune system mediated by a bacterial DNA through TLR9... - Structural and functional differences in two cyclic bacteriocins with the same sequences produced by lactobacilliYasushi Kawai
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
Appl Environ Microbiol 70:2906-11. 2004..This is the first report that characteristics of native bacteriocins produced by wild lactobacillus strains having the same structural genes are influenced by a difference in D-amino acid contents in the molecules... - Dextran from Leuconostoc mesenteroides augments immunostimulatory effects by the introduction of phosphate groupsToshihiro Sato
NOF Corporation, Yebisu Garden Place Tower, 20-3 Ebisu 4-chome, Shibuya-ku, Tokyo 150-6019, Japan
J Food Prot 67:1719-24. 2004..The levels of mRNA expression of gamma interferon and interleukin-10 on murine splenocytes were also increased by the stimulation. These results demonstrate that dextran exerts immunostimulation by the introduction of phosphate groups... - An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitroNoritoshi Takahashi
Food Research and Development Laboratory, Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, Japan
FEMS Immunol Med Microbiol 46:461-9. 2006..The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536... - Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine modelNoritoshi Takahashi
Food Research and Development Laboratory, Morinaga Milk Industry Co, Ltd, Zama, Japan
Biosci Biotechnol Biochem 70:2013-7. 2006..ODN BL07S increased Th1 cytokine and decreased Th2 cytokine production in splenocytes. These results suggest that immunostimulatory ODNs are potentially associated with the antiallergic effects of probiotics...