Research Topics
| Keietsu AbeSummaryAffiliation: Tohoku University Country: Japan Publications
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Detail Information
Publications
Novel hydrophobic surface binding protein, HsbA, produced by Aspergillus oryzaeShinsaku Ohtaki
Laboratory of Molecular Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumi-dori, Aobaku, Sendai 981-8555, Japan
Appl Environ Microbiol 72:2407-13. 2006..Since HsbA is not a hydrophobin, A. oryzae may use several types of proteins to recruit lytic enzymes to the surface of hydrophobic solid materials and promote their degradation...- Plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reactionKeietsu Abe
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
J Bacteriol 184:2906-13. 2002.... 
Impact of Aspergillus oryzae genomics on industrial production of metabolitesKeietsu Abe
Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555, Japan
Mycopathologia 162:143-53. 2006..This article describes recent progress of A . oryzae genomics and its impact on industrial production of enzymes, metabolites, and bioprocesses...- Novel reporter gene expression systems for monitoring activation of the Aspergillus nidulans HOG pathwayKentaro Furukawa
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Biosci Biotechnol Biochem 71:1724-30. 2007..These results indicate that the reporter activity is controlled via HogA activation. Furthermore, we present possible applications of the reporter systems in screening new antifungal compounds... - The fungal hydrophobin RolA recruits polyesterase and laterally moves on hydrophobic surfacesToru Takahashi
Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai 985-8555, Japan
Mol Microbiol 57:1780-96. 2005..A fluorescence recovery after photobleaching experiment on PBSA films coated with FITC-labelled RolA suggested that RolA moves laterally on the film. We discuss the novel molecular functions of RolA with regard to plastic degradation... 
Transcriptional profiling for Aspergillusnidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stressDaisuke Hagiwara
New Industry Creation Hatchery Center, Tohoku University, 6 6 10 Aoba, Aramaki, Aoba ku, Sendai 980 8579, Japan
Fungal Genet Biol 46:868-78. 2009..The complexity of the stress response in the His-Asp phosphorelay system followed by the HogA MAPK cascade is discussed...- MpkA-Dependent and -independent cell wall integrity signaling in Aspergillus nidulansTomonori Fujioka
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1 1 Amamiya, Tsutsumi dori, Sendai 981 8555, Japan
Eukaryot Cell 6:1497-510. 2007..The transcriptional regulation of mpkA and of CWGs via CWIS in A. nidulans differs significantly from that in S. cerevisiae... - Topology of AspT, the aspartate:alanine antiporter of Tetragenococcus halophilus, determined by site-directed fluorescence labelingKei Nanatani
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555 Japan
J Bacteriol 189:7089-97. 2007.... - Disordered cell integrity signaling caused by disruption of the kexB gene in Aspergillus oryzaeOsamu Mizutani
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1 1 Amamiya, Tsutsumi dori, Aobaku, Sendai 981 8555, Japan
Eukaryot Cell 3:1036-48. 2004.... - Use of the Aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genesJunichiro Marui
ABE project, New Industry Creation Hatchery Center, Tohoku University, 6 6 10 Aoba, Aramaki, Aoba ku, Sendai, Miyagi 980 8579, Japan
Appl Microbiol Biotechnol 87:1829-40. 2010..Furthermore, the reporter strain was shown to be technically applicable for identifying novel target genes of antifungal drugs triggering perturbation of fungal microtubules or membrane integrity... - A defect of LigD (human Lig4 homolog) for nonhomologous end joining significantly improves efficiency of gene-targeting in Aspergillus oryzaeOsamu Mizutani
Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
Fungal Genet Biol 45:878-89. 2008..oryzae unique MAPK (mpkD). Such results show that the ligD disruptant system is an extremely convenient genetic background for gene-targeting in A. oryzae... - Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzaeHiroshi Maeda
Tohoku Technoarch, 2-1-1 Katahira, Aoba-ku, Sendai, 980-8577, Japan
Appl Microbiol Biotechnol 67:778-88. 2005..Determination of the affinities for different chemicals indicated that the most preferred substrate for the enzyme would consist of butyric acid and n-hexanol... - Pepper beta-galactosidase 1 (PBG1) plays a significant role in fruit ripening in bell pepper (Capsicum annuum)Satoshi Ogasawara
Graduate School of Agricultural Sciences, Tohoku University, Sendai, Japan
Biosci Biotechnol Biochem 71:309-22. 2007..Based on these results, it appears that the hydrolysis of galactose residues in pectic substances is the first event in the ripening process in bell pepper fruit... - Cloning and expression of the exo-beta-D-1,3-glucanase gene (exgS) from Aspergillus saitoiKen Oda
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Biosci Biotechnol Biochem 66:1587-90. 2002..The N-terminal amino acid sequences of the recombinant exo-1,3-beta-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi... - Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tagsHiroshi Maeda
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumi-dori, 981-8555 Sendai, Japan
Appl Microbiol Biotechnol 65:74-83. 2004..The low expression levels of catabolic genes in the wheat-bran culture may release catabolite repression, consequently leading to the rich expression profiles of the hydrolytic enzymes... - Isolation and functional analysis of a gene, tcsB, encoding a transmembrane hybrid-type histidine kinase from Aspergillus nidulansKentaro Furukawa
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumi-dori, Sendai 981-8555, Japan
Appl Environ Microbiol 68:5304-10. 2002..Our results suggest that A. nidulans has more complex and robust osmoregulatory systems than the yeast SLN1-HOG1 MAPK cascade... - Substrate specificity of the aspartate:alanine antiporter (AspT) of Tetragenococcus halophilus in reconstituted liposomesAyako Sasahara
Department of Microbial Biotechnology, Laboratory of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
J Biol Chem 286:29044-52. 2011..Taken together, these kinetic data suggest that the putative binding sites of L-aspartate and L-alanine are independently located in the substrate translocation pathway of AspT... - Functional analysis of C2H2 zinc finger transcription factor CrzA involved in calcium signaling in Aspergillus nidulansDaisuke Hagiwara
ABE project, New Industry Creation Hatchery Center, Tohoku University, 6 6 10 Aoba, Aramaki, Aoba ku, Sendai, Japan
Curr Genet 54:325-38. 2008..nidulans. Moreover, a crzA/hogA double mutant showed hypersensitivity to osmotic stress, indicating the importance of calcium homeostasis for adaptation to osmotic stress, a universal stress in filamentous fungi... - Structural and functional importance of transmembrane domain 3 (TM3) in the aspartate:alanine antiporter AspT: topology and function of the residues of TM3 and oligomerization of AspTKei Nanatani
Department of Molecular and Cell Biology, Tohoku University, Sendai, Japan
J Bacteriol 191:2122-32. 2009..In addition, nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses and a glutaraldehyde cross-linking assay suggest that functional AspT forms homo-oligomers as a functional unit... - Molecular cloning and characterization of a novel gamma-CGTase from alkalophilic Bacillus spKyoko Hirano
Laboratory of Molecular Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Japan
Appl Microbiol Biotechnol 70:193-201. 2006..CGTase825-6 should be a useful tool to produce gamma-CD and to study the differences of producing mechanisms between gamma-CD and beta-CD... - Aspergillus nidulans HOG pathway is activated only by two-component signalling pathway in response to osmotic stressKentaro Furukawa
Graduate School of Agricultural Sciences, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
Mol Microbiol 56:1246-61. 2005..nidulans, although mpkC is dispensable in osmoadaptation. In this study, we discuss the differences between the AnHOG and the yeast HOG pathways... - Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungusAkira Yoshimi
New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan
PLoS ONE 8:e54893. 2013..Taken together, our data demonstrate that the two AGS genes are dispensable in A. nidulans, but that AgsB is required for normal growth characteristics under liquid culture conditions and is the major AGS in this species... - chsZ, a gene for a novel class of chitin synthase from Aspergillus oryzaeYuko Chigira
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
Curr Genet 41:261-7. 2002..oryzae ChsZ, P. brasiliensis Chs4 and U. maydis Chs6. Expression analysis suggested that the regulation of chsZ expression is distinct from that of chsY expression... - Hydrophobic interactions between the secondary structures on the molecular surface reinforce the alkaline stability of serine proteaseYuki Oguchi
Laboratory of Molecular Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
Biotechnol Lett 28:1383-91. 2006..This indicated that different strategies were necessary to improve the alkaline stability and thermostability of the protein... - Membrane topology of the electrogenic aspartate-alanine antiporter AspT of Tetragenococcus halophilusKei Nanatani
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan
Biochem Biophys Res Commun 328:20-6. 2005..These results demonstrated a unique 2D-structure of AspT as the novel AAE family... - Characterization of NikA histidine kinase and two response regulators with special reference to osmotic adaptation and asexual development in Aspergillus nidulansDaisuke Hagiwara
New Industry Creation Hatchery Center, Tohoku University, Aramaki, Sendai, Japan
Biosci Biotechnol Biochem 73:1566-71. 2009..Based on these results, a general insight into the A. nidulans His-Asp phosphorelay network is also discussed... - Cloning and expression of an endo-1,6-beta-D-glucanase gene (neg1) from Neurospora crassaSeiji Oyama
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Biosci Biotechnol Biochem 66:1378-81. 2002..The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-beta-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi... - Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR geneTadashi Takahashi
Research and Development Division, Kikkoman Corporation, 399 Noda, Noda City 278 0037, Japan
Appl Environ Microbiol 68:3737-43. 2002..sojae can be attributed, at least partially, to the premature termination defect in aflRs, which deletes the C-terminal transcription activation domain that is critical for the expression of aflatoxin biosynthetic genes... - A polymerase chain reaction-based method for cloning novel members of a gene family using a combination of degenerate and inhibitory primersSumiko Kunihiro
Institute of Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan
Gene 289:177-84. 2002.... - Genome sequencing and analysis of Aspergillus oryzaeMasayuki Machida
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
Nature 438:1157-61. 2005..Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation... - Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatusWilliam C Nierman
The Institute for Genomic Research, Rockville, Maryland 20850, USA
Nature 438:1151-6. 2005..The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus... - Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivationKoichi Tamano
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
Fungal Genet Biol 45:139-51. 2008..These results strongly suggest that the genes on NSBs play an important role on solid-state fermentation... - Understanding nonaflatoxigenicity of Aspergillus sojae: a windfall of aflatoxin biosynthesis researchPerng Kuang Chang
Food and Feed Safety Research Unit, Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 1100 Robert E Lee Boulevard, New Orleans, LA 70124, USA
Appl Microbiol Biotechnol 76:977-84. 2007..Both are required for gene expression. In addition, a defect in the polyketide synthase gene also contributes to its nonaflatoxigenicity... - The beta-1,3-exoglucanase gene exgA (exg1) of Aspergillus oryzae is required to catabolize extracellular glucan, and is induced in growth on a solid surfaceKoichi Tamano
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan
Biosci Biotechnol Biochem 71:926-34. 2007..These data suggest that ExgA plays a role in beta-glucan utilization, but is not much involved in cell wall beta-glucan remodeling... - The SskA and SrrA response regulators are implicated in oxidative stress responses of hyphae and asexual spores in the phosphorelay signaling network of Aspergillus nidulansDaisuke Hagiwara
Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Biosci Biotechnol Biochem 71:1003-14. 2007..These results together are indicative of the physiological importance of the His-Asp phosphorelay signaling network involving the SskA and SrrA response regulators... - Genomics of Aspergillus oryzaeTetsuo Kobayashi
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Biosci Biotechnol Biochem 71:646-70. 2007..oryzae genome. Besides the various already established merits of A. oryzae for industrial uses, the genome sequence and the abundance of metabolic genes should significantly accelerate the biotechnological use of A. oryzae in industry... - Characterization of the NikA histidine kinase implicated in the phosphorelay signal transduction of Aspergillus nidulans, with special reference to fungicide responsesDaisuke Hagiwara
Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Biosci Biotechnol Biochem 71:844-7. 2007.... - Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditionsTakeshi Akao
National Research Institute of Brewing, 3 7 1 Kagamiyama, Higashi Hiroshima, Hiroshima 739 0046, Japan
DNA Res 14:47-57. 2007..In addition, contig sequences were compared with sequence sets in eukaryotic orthologous groups (KOGs), and classified into the KOG functional categories...